pxf-1 cDNA ORF clone, Caenorhabditis elegans

The following pxf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pxf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa343606 NM_001268538.2
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OCa342237 NM_001028218.5
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa342941 NM_001268537.2
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa342942 NM_001372914.1
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa342942 NM_001268539.1
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342943 NM_001268540.1
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa342943 NM_001372915.1
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa342944 NM_001268536.2
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa343098 NM_001028219.3
Latest version!
Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OCa343098 NM_001028219.2 Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342237 NM_001028218.4 Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCa343606 NM_001268538.1 Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OCa342944 NM_001268536.1 Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCa342941 NM_001268537.1 Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa343606
    Clone ID Related Accession (Same CDS sequence) NM_001268538.1 , NM_001268538.2
    Accession Version NM_001268538.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT 
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255467.1
    CDS1..258
    Translation

    Target ORF information:

    RefSeq Version NM_001268538.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT 
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343606
    Clone ID Related Accession (Same CDS sequence) NM_001268538.1 , NM_001268538.2
    Accession Version NM_001268538.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268538.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT 
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255467.1
    CDS1..258
    Translation

    Target ORF information:

    RefSeq Version NM_001268538.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268538.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT 
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342237
    Clone ID Related Accession (Same CDS sequence) NM_001028218.4 , NM_001028218.5
    Accession Version NM_001028218.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001028218.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGATAATGGG CGCCGGCAAC GATCTGGATC CGAAGGAAGA
    TTTGATAATA TTCCGCCATC AACCTTCTAT CTAACATCCG ACGGTCTTAC TGTATCACCC
    AGACAATCGT TGTCTGTTGT GATTCCAACA CATCCTCATG GCCACTCTCC AACATCGCCA
    CGGTGCCGCT CGCGATCACC GGCATCTTCC GGGTGTTCTT CCTTCTCCAC CATTGCCTCA
    ATCGCCGCTA CCTCGATGGC AGCCGCACCT TCGGCATTTG TCTCCAATCC GTACCAACAC
    CACCAAACCG TCCGTGGACA TGTCATAGGA CATCGTCCAA TGCCAATCGT CACTAGTGGT
    TCAGCCACAC TCCCGAATCA TGTTTCTCCA CGAGGTTTAC CACCGAAATC TCGTCCTACA
    ATCCTACCTG GTTCACACAC CAATTCTTCA TCAAGAATGG GTACTATCAA AGAAGCTACA
    TTCCTGACAT CTGAACAAGT ATCGCGAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001023389.1
    CDS1..4413
    Translation

    Target ORF information:

    RefSeq Version NM_001028218.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001028218.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGATAATGGG CGCCGGCAAC GATCTGGATC CGAAGGAAGA
    TTTGATAATA TTCCGCCATC AACCTTCTAT CTAACATCCG ACGGTCTTAC TGTATCACCC
    AGACAATCGT TGTCTGTTGT GATTCCAACA CATCCTCATG GCCACTCTCC AACATCGCCA
    CGGTGCCGCT CGCGATCACC GGCATCTTCC GGGTGTTCTT CCTTCTCCAC CATTGCCTCA
    ATCGCCGCTA CCTCGATGGC AGCCGCACCT TCGGCATTTG TCTCCAATCC GTACCAACAC
    CACCAAACCG TCCGTGGACA TGTCATAGGA CATCGTCCAA TGCCAATCGT CACTAGTGGT
    TCAGCCACAC TCCCGAATCA TGTTTCTCCA CGAGGTTTAC CACCGAAATC TCGTCCTACA
    ATCCTACCTG GTTCACACAC CAATTCTTCA TCAAGAATGG GTACTATCAA AGAAGCTACA
    TTCCTGACAT CTGAACAAGT ATCGCGAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342237
    Clone ID Related Accession (Same CDS sequence) NM_001028218.4 , NM_001028218.5
    Accession Version NM_001028218.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001028218.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGATAATGGG CGCCGGCAAC GATCTGGATC CGAAGGAAGA
    TTTGATAATA TTCCGCCATC AACCTTCTAT CTAACATCCG ACGGTCTTAC TGTATCACCC
    AGACAATCGT TGTCTGTTGT GATTCCAACA CATCCTCATG GCCACTCTCC AACATCGCCA
    CGGTGCCGCT CGCGATCACC GGCATCTTCC GGGTGTTCTT CCTTCTCCAC CATTGCCTCA
    ATCGCCGCTA CCTCGATGGC AGCCGCACCT TCGGCATTTG TCTCCAATCC GTACCAACAC
    CACCAAACCG TCCGTGGACA TGTCATAGGA CATCGTCCAA TGCCAATCGT CACTAGTGGT
    TCAGCCACAC TCCCGAATCA TGTTTCTCCA CGAGGTTTAC CACCGAAATC TCGTCCTACA
    ATCCTACCTG GTTCACACAC CAATTCTTCA TCAAGAATGG GTACTATCAA AGAAGCTACA
    TTCCTGACAT CTGAACAAGT ATCGCGAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001023389.1
    CDS12..4424
    Translation

    Target ORF information:

    RefSeq Version NM_001028218.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001028218.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGATAATGGG CGCCGGCAAC GATCTGGATC CGAAGGAAGA
    TTTGATAATA TTCCGCCATC AACCTTCTAT CTAACATCCG ACGGTCTTAC TGTATCACCC
    AGACAATCGT TGTCTGTTGT GATTCCAACA CATCCTCATG GCCACTCTCC AACATCGCCA
    CGGTGCCGCT CGCGATCACC GGCATCTTCC GGGTGTTCTT CCTTCTCCAC CATTGCCTCA
    ATCGCCGCTA CCTCGATGGC AGCCGCACCT TCGGCATTTG TCTCCAATCC GTACCAACAC
    CACCAAACCG TCCGTGGACA TGTCATAGGA CATCGTCCAA TGCCAATCGT CACTAGTGGT
    TCAGCCACAC TCCCGAATCA TGTTTCTCCA CGAGGTTTAC CACCGAAATC TCGTCCTACA
    ATCCTACCTG GTTCACACAC CAATTCTTCA TCAAGAATGG GTACTATCAA AGAAGCTACA
    TTCCTGACAT CTGAACAAGT ATCGCGAGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342941
    Clone ID Related Accession (Same CDS sequence) NM_001268537.1 , NM_001268537.2
    Accession Version NM_001268537.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGATA ATGGGCGCCG GCAACGATCT GGATCCGAAG GAAGATTTGA TAATATTCCG
    CCATCAACCT TCTATCTAAC ATCCGACGGT CTTACTGTAT CACCCAGACA ATCGTTGTCT
    GTTGTGATTC CAACACATCC TCATGGCCAC TCTCCAACAT CGCCACGGTG CCGCTCGCGA
    TCACCGGCAT CTTCCGGGTG TTCTTCCTTC TCCACCATTG CCTCAATCGC CGCTACCTCG
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255466.1
    CDS1..3918
    Translation

    Target ORF information:

    RefSeq Version NM_001268537.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGATA ATGGGCGCCG GCAACGATCT GGATCCGAAG GAAGATTTGA TAATATTCCG
    CCATCAACCT TCTATCTAAC ATCCGACGGT CTTACTGTAT CACCCAGACA ATCGTTGTCT
    GTTGTGATTC CAACACATCC TCATGGCCAC TCTCCAACAT CGCCACGGTG CCGCTCGCGA
    TCACCGGCAT CTTCCGGGTG TTCTTCCTTC TCCACCATTG CCTCAATCGC CGCTACCTCG
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342941
    Clone ID Related Accession (Same CDS sequence) NM_001268537.1 , NM_001268537.2
    Accession Version NM_001268537.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268537.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGATA ATGGGCGCCG GCAACGATCT GGATCCGAAG GAAGATTTGA TAATATTCCG
    CCATCAACCT TCTATCTAAC ATCCGACGGT CTTACTGTAT CACCCAGACA ATCGTTGTCT
    GTTGTGATTC CAACACATCC TCATGGCCAC TCTCCAACAT CGCCACGGTG CCGCTCGCGA
    TCACCGGCAT CTTCCGGGTG TTCTTCCTTC TCCACCATTG CCTCAATCGC CGCTACCTCG
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255466.1
    CDS1..3918
    Translation

    Target ORF information:

    RefSeq Version NM_001268537.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268537.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGATA ATGGGCGCCG GCAACGATCT GGATCCGAAG GAAGATTTGA TAATATTCCG
    CCATCAACCT TCTATCTAAC ATCCGACGGT CTTACTGTAT CACCCAGACA ATCGTTGTCT
    GTTGTGATTC CAACACATCC TCATGGCCAC TCTCCAACAT CGCCACGGTG CCGCTCGCGA
    TCACCGGCAT CTTCCGGGTG TTCTTCCTTC TCCACCATTG CCTCAATCGC CGCTACCTCG
    ATGGCAGCCG CACCTTCGGC ATTTGTCTCC AATCCGTACC AACACCACCA AACCGTCCGT
    GGACATGTCA TAGGACATCG TCCAATGCCA ATCGTCACTA GTGGTTCAGC CACACTCCCG
    AATCATGTTT CTCCACGAGG TTTACCACCG AAATCTCGTC CTACAATCCT ACCTGGTTCA
    CACACCAATT CTTCATCAAG AATGGGTACT ATCAAAGAAG CTACATTCCT GACATCTGAA
    CAAGTATCGC GAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342942
    Clone ID Related Accession (Same CDS sequence) NM_001268539.1 , NM_001372914.1
    Accession Version NM_001268539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGACGTGCT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255468.1
    CDS1..3567
    Translation

    Target ORF information:

    RefSeq Version NM_001268539.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGACGTGCT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342942
    Clone ID Related Accession (Same CDS sequence) NM_001268539.1 , NM_001372914.1
    Accession Version NM_001372914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGACGTGCT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359774.1
    CDS1..3567
    Translation

    Target ORF information:

    RefSeq Version NM_001372914.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGACGTGCT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342943
    Clone ID Related Accession (Same CDS sequence) NM_001372915.1 , NM_001268540.1
    Accession Version NM_001372915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGACG TGCTCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359775.1
    CDS1..3441
    Translation

    Target ORF information:

    RefSeq Version NM_001372915.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGACG TGCTCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342943
    Clone ID Related Accession (Same CDS sequence) NM_001372915.1 , NM_001268540.1
    Accession Version NM_001268540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGACG TGCTCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255469.1
    CDS1..3441
    Translation

    Target ORF information:

    RefSeq Version NM_001268540.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCAGTTG ATTCAATTGC AATGCCGCCA CCTCCAGTTC CACCTCGTCC TCTACGTCTT 
    CCACAAACTG CAGCTAAAGG CCCAGCTCCT CTGCCGCCAC GTGGTTTACC TAGAACATAT
    CCATTGGATT TTCCTGTAGA TATTCCAACA ACGTCATCAT CATCATCAAA CACTTCATAT
    AATGATCAAC ATCGTAGTCA AGTATATTTG AATGGATTAT CCGCTGATGA AGATACTCTT
    GTTCGAGTGA AGCATAGAAG AGAGAAAAGC AATTCAGTTG GAGGACAAGC CCAGAATGGA
    ATTTCAACTG CTCGGAGGCT TCGAGGAAGA AGCACAGCAT CATCTACAAC TACTGAAGGT
    GAAACAGCTT CCAACGAAGG AGCAGATTCA GACGAAGATG AAGGGAGTAT GCCTTCACAA
    GAATCAAGTA GTGGAGGATT CATGGATCTG CGAGATTCTG TCCGAGAATG TCTTGAAAAG
    GAACCTTCAG AACGAAATTC AGAAGATCTT GCAGTTTTAC TCGATTTCAT GCAACATATG
    TCCGCATTTG CCGCTTTGCC GATGTCAATA AAACGTCAGT TATGCTTGAA AATGGTTTTT
    GCGGTAGTAA ACGATGCGGG TACGGTAGTT CTTGCTCACA ACGAGAAACT CGATTCATGG
    TCTGTAATAG TGAATGGATG TGTAGAAGTT GTAAAACCAT CTGGTGAGCG TGTTGAATAC
    AAGCTCGGAG ATTCATTTGG TGCAGAACCA ACACCTGCAA CACAAATTCA TATCGGTGAA
    ATGAGAACAA TGGTGGATGA TTGTGAATTC GTACTTGTTG AACATCGAGA TTTTTGTTCA
    ATTATGTCGA CAATTGGTGA TCATATTGAA AAAGATCGAG ACGGTTTGAC AGGAGAAGTT
    GTGTCAGAAG TTGAACGAAG GACTGTTGGA ACTCATTGTG GACAAGTGCT TATCAAAGGA
    AAACCAGACA AATTAATACA CCATTTAGTG GATGAACGAG ATCATAATGT TGATCCACAC
    TATGTCGACG ATTTTCTATT GACCTATCGA GTTTTCATAA GAGATCCAAC TACGATTTTC
    GAGAAATTGA TGCTCTGGTT TGCCGATTCT ATATATCGAG ATAAAGTTGC AAGATTGGTT
    CTTCTATGGG TTAATAATCA TTTTAATGAT TTCGAGACGA ACGATGAGAT GTGGAATCTA
    TTAGAACGTT TTGAAGGAGC ATTAGAAAGA GATGGAATGC ATTCTCAACT ATCTTTATTG
    AATATTGCAT GTTCTGTAAA AGCAAAACCT CGACAAGTGA TATTAACAAG GAGAAAAGAT
    GACAAAATGA TGATGAGATT AGTTGGAGGT CAAGAATCAG GCAACTCTGT ATACGTGGCC
    GAAGTCTTCC CAGATACGTC AGCAGCGAGA GAAGGTGTGA AGAGAGCTGA TGAAATGTTA
    GAAGTGAATC AACAATCAGC TAAATATTTA TCAGCCAAAA AAGCGGAAGA TCTACTAACG
    GGGTCTCTGT CATTAACTTT GATGCTCAAA AATAATGTTC TTGGGTACAA GGAGACAATT
    GGAAAAATTG AACATAATAA GCCTAAAAAT GGAACATCAA GATCTGGTGC CGGAATTCCA
    ATGGTAATTC CAGTTCACAA AACTTCAATA ACTGGAAAGA AATCATCGAC GACAAGTAGC
    AAATCGGGTA TGATGGAAAA ATTGATGACA ATATTGAAAT CATCAAAAGA AGATTCAATG
    GATTTCACTG ATGAAGCTAA GATATCATCA GCCGATCTTC GTCCAAGTCG ATCCAATCCT
    GATATCACTA GCATATCACA GTACTACGGA CCAGTACGAA GCGAATGTCC TGAACACGTT
    CTGAAGATTT ATCGAAATGA TCAGACATTC AAATATCTGC CAGTTTACAA AGAAACGTCT
    GCTCAGAATG TTGTACAACT GGCATTGCAA GAGTTTAATA TGACAGCAGA AGGAAGTCCA
    GAATGGTCAC TTTGTGAATG TACTGTTACG ATTGATGGAG TTATAAAACA GAGAAGGTTA
    CCACCACAAA TGGAAAATTT GGCTGAAAGA ATAGCTTTGA ATTCGAGATA TTATCTGAAA
    AACAATAGTC GATCTGAACC TCTTGTACCT GATGAATTGG CTCCCGAACT TTTGAAAGAG
    GCCCAAACTC AACTGCTCAG TTTAAATGCT CAAGTCGTTG CTGCTCAACT AACTTTGCAA
    GACTTTTCAG TTTTTTCAGC AATTGAACCA ACAGAATTTC TAGATAACTT ATTCAAGCTG
    GATTCCAAGT ACGGCTCCCC AAAACTTGAA GAATTCGAAC AGTTATTCAA TCGTGAAATG
    TGGTGGGTGG CGACAGAGAT TTGCACAGAA AGACATGTTC AAAAACGTGC AAAACTGATT
    AAAAAGTTTA TAAAAGTGGC GAGATATTGT CGGGATCTTC GAAATTTCAA TTCAATGTTT
    GCTATTATGA GTGGACTTGA TAAGCCAGCA GTTCGTAGGT TGCATTCCAG TTGGGAACGG
    GTTTCGAGTA AATACATTCG TATGCTTGAT GAAATCCATC AACTTGTCGA TCCTTCTCGT
    AATATGTCAA AGTATCGTCA ACATCTTGCT GAAGTCGCCC AAGAACCGCC TGTTGTCCCA
    ATTTATCCTG TAATCAAAAA AGATCTCACA TTTGCTCACG ATGGTAATGC AACATATTCT
    GAAAAACTAA TCAATTTCGA GAAGCTTCGA CTTATTGCAA AATCAATTCG AGGTGTAATG
    AAATTGAGTA GTGCACCTTA TGAAATTGCT AGTATGGCAG AGAGGAGTGG TGGAGTTGTA
    ATGGACGCTC TTCTTCATAT GAATTCATTC GAAAATTCAA ATGTAGCCAC AATGAGGAAG
    GGAATGAGTG GAAAACAGAA TCAGCCACGA AAAAAAGTTT ATGAACAAGC TCTTATGGTA
    CGGAAAGTGA AATCATATCT CGAAGGATTA CATGTTGTTG ATAATGAAAT GGAATTGGAT
    TCAATGAGTT ATGACATTGA ACCACAAGTT CAGACAGCTC ACCGTGGTGC TAATTCATCA
    TCGACTGCAA ATATTCGTCG TGTTCCATCT CCAACTCCAT CGTCACTTTC CTCTCAATCA
    GCCGGGTCAG CCGATCAATC ATCTCGGCAT CGTCTTCTTT TCAATGGAAC CGGCTCAATT
    TCGTCAGCTG GTGGTGGTTC TAAATTTGGA GTCGAATCTC CACAAGCTGT ACAAAAAATG
    CTGTCACTTG TTCAGAATTC CAAAGTTAAA GGTGCTCCAC CTCAAATCAC ATCTCCATCC
    ACATCAGCAA GATCCTCTCT TCAACGGAAT ATGCCACGTG TAACTGGTCG ACAAGCGACA
    TCCTCGGCTC AAGGGCCAGT TCAACTGAAT GAAGAGACGT CTACGGTTAC GACATATTAT
    CAATCAGACG TGCTCATCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342944
    Clone ID Related Accession (Same CDS sequence) NM_001268536.1 , NM_001268536.2
    Accession Version NM_001268536.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGATAATGG GCGCCGGCAA CGATCTGGAT CCGAAGGAAG ATTTGATAAT
    ATTCCGCCAT CAACCTTCTA TCTAACATCC GACGGTCTTA CTGTATCACC CAGACAATCG
    TTGTCTGTTG TGATTCCAAC ACATCCTCAT GGCCACTCTC CAACATCGCC ACGGTGCCGC
    TCGCGATCAC CGGCATCTTC CGGGTGTTCT TCCTTCTCCA CCATTGCCTC AATCGCCGCT
    ACCTCGATGG CAGCCGCACC TTCGGCATTT GTCTCCAATC CGTACCAACA CCACCAAACC
    GTCCGTGGAC ATGTCATAGG ACATCGTCCA ATGCCAATCG TCACTAGTGG TTCAGCCACA
    CTCCCGAATC ATGTTTCTCC ACGAGGTTTA CCACCGAAAT CTCGTCCTAC AATCCTACCT
    GGTTCACACA CCAATTCTTC ATCAAGAATG GGTACTATCA AAGAAGCTAC ATTCCTGACA
    TCTGAACAAG TATCGCGAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255465.1
    CDS1..4044
    Translation

    Target ORF information:

    RefSeq Version NM_001268536.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGATAATGG GCGCCGGCAA CGATCTGGAT CCGAAGGAAG ATTTGATAAT
    ATTCCGCCAT CAACCTTCTA TCTAACATCC GACGGTCTTA CTGTATCACC CAGACAATCG
    TTGTCTGTTG TGATTCCAAC ACATCCTCAT GGCCACTCTC CAACATCGCC ACGGTGCCGC
    TCGCGATCAC CGGCATCTTC CGGGTGTTCT TCCTTCTCCA CCATTGCCTC AATCGCCGCT
    ACCTCGATGG CAGCCGCACC TTCGGCATTT GTCTCCAATC CGTACCAACA CCACCAAACC
    GTCCGTGGAC ATGTCATAGG ACATCGTCCA ATGCCAATCG TCACTAGTGG TTCAGCCACA
    CTCCCGAATC ATGTTTCTCC ACGAGGTTTA CCACCGAAAT CTCGTCCTAC AATCCTACCT
    GGTTCACACA CCAATTCTTC ATCAAGAATG GGTACTATCA AAGAAGCTAC ATTCCTGACA
    TCTGAACAAG TATCGCGAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342944
    Clone ID Related Accession (Same CDS sequence) NM_001268536.1 , NM_001268536.2
    Accession Version NM_001268536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268536.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGATAATGG GCGCCGGCAA CGATCTGGAT CCGAAGGAAG ATTTGATAAT
    ATTCCGCCAT CAACCTTCTA TCTAACATCC GACGGTCTTA CTGTATCACC CAGACAATCG
    TTGTCTGTTG TGATTCCAAC ACATCCTCAT GGCCACTCTC CAACATCGCC ACGGTGCCGC
    TCGCGATCAC CGGCATCTTC CGGGTGTTCT TCCTTCTCCA CCATTGCCTC AATCGCCGCT
    ACCTCGATGG CAGCCGCACC TTCGGCATTT GTCTCCAATC CGTACCAACA CCACCAAACC
    GTCCGTGGAC ATGTCATAGG ACATCGTCCA ATGCCAATCG TCACTAGTGG TTCAGCCACA
    CTCCCGAATC ATGTTTCTCC ACGAGGTTTA CCACCGAAAT CTCGTCCTAC AATCCTACCT
    GGTTCACACA CCAATTCTTC ATCAAGAATG GGTACTATCA AAGAAGCTAC ATTCCTGACA
    TCTGAACAAG TATCGCGAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255465.1
    CDS1..4044
    Translation

    Target ORF information:

    RefSeq Version NM_001268536.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGATTGTGA TTGACTATCC AACGGAACCC CAATCGAATG GAATGTCACC ACGAACACCT 
    CCTCGAGGAA TTCATCATTC AGGAGAACCA GTTCATCAGA AAACTCCTAG AAAATCGGCA
    CCAAATATGT CAGTTGATTC AATTGCAATG CCGCCACCTC CAGTTCCACC TCGTCCTCTA
    CGTCTTCCAC AAACTGCAGC TAAAGGCCCA GCTCCTCTGC CGCCACGTGG TTTACCTAGA
    ACATATCCAT TGGATTTTCC TGTAGATATT CCAACAACGT CATCATCATC ATCAAACACT
    TCATATAATG ATCAACATCG TAGTCAAGTA TATTTGAATG GATTATCCGC TGATGAAGAT
    ACTCTTGTTC GAGTGAAGCA TAGAAGAGAG AAAAGCAATT CAGTTGGAGG ACAAGCCCAG
    AATGGAATTT CAACTGCTCG GAGGCTTCGA GGAAGAAGCA CAGCATCATC TACAACTACT
    GAAGGTGAAA CAGCTTCCAA CGAAGGAGCA GATTCAGACG AAGATGAAGG GAGTATGCCT
    TCACAAGAAT CAAGTAGTGG AGGATTCATG GATCTGCGAG ATTCTGTCCG AGAATGTCTT
    GAAAAGGAAC CTTCAGAACG AAATTCAGAA GATCTTGCAG TTTTACTCGA TTTCATGCAA
    CATATGTCCG CATTTGCCGC TTTGCCGATG TCAATAAAAC GTCAGTTATG CTTGAAAATG
    GTTTTTGCGG TAGTAAACGA TGCGGGTACG GTAGTTCTTG CTCACAACGA GAAACTCGAT
    TCATGGTCTG TAATAGTGAA TGGATGTGTA GAAGTTGTAA AACCATCTGG TGAGCGTGTT
    GAATACAAGC TCGGAGATTC ATTTGGTGCA GAACCAACAC CTGCAACACA AATTCATATC
    GGTGAAATGA GAACAATGGT GGATGATTGT GAATTCGTAC TTGTTGAACA TCGAGATTTT
    TGTTCAATTA TGTCGACAAT TGGTGATCAT ATTGAAAAAG ATCGAGACGG TTTGACAGGA
    GAAGTTGTGT CAGAAGTTGA ACGAAGGACT GTTGGAACTC ATTGTGGACA AGTGCTTATC
    AAAGGAAAAC CAGACAAATT AATACACCAT TTAGTGGATG AACGAGATCA TAATGTTGAT
    CCACACTATG TCGACGATTT TCTATTGACC TATCGAGTTT TCATAAGAGA TCCAACTACG
    ATTTTCGAGA AATTGATGCT CTGGTTTGCC GATTCTATAT ATCGAGATAA AGTTGCAAGA
    TTGGTTCTTC TATGGGTTAA TAATCATTTT AATGATTTCG AGACGAACGA TGAGATGTGG
    AATCTATTAG AACGTTTTGA AGGAGCATTA GAAAGAGATG GAATGCATTC TCAACTATCT
    TTATTGAATA TTGCATGTTC TGTAAAAGCA AAACCTCGAC AAGTGATATT AACAAGGAGA
    AAAGATGACA AAATGATGAT GAGATTAGTT GGAGGTCAAG AATCAGGCAA CTCTGTATAC
    GTGGCCGAAG TCTTCCCAGA TACGTCAGCA GCGAGAGAAG GTGTGAAGAG AGCTGATGAA
    ATGTTAGAAG TGAATCAACA ATCAGCTAAA TATTTATCAG CCAAAAAAGC GGAAGATCTA
    CTAACGGGGT CTCTGTCATT AACTTTGATG CTCAAAAATA ATGTTCTTGG GTACAAGGAG
    ACAATTGGAA AAATTGAACA TAATAAGCCT AAAAATGGAA CATCAAGATC TGGTGCCGGA
    ATTCCAATGG TAATTCCAGT TCACAAAACT TCAATAACTG GAAAGAAATC ATCGACGACA
    AGTAGCAAAT CGGGTATGAT GGAAAAATTG ATGACAATAT TGAAATCATC AAAAGAAGAT
    TCAATGGATT TCACTGATGA AGCTAAGATA TCATCAGCCG ATCTTCGTCC AAGTCGATCC
    AATCCTGATA TCACTAGCAT ATCACAGTAC TACGGACCAG TACGAAGCGA ATGTCCTGAA
    CACGTTCTGA AGATTTATCG AAATGATCAG ACATTCAAAT ATCTGCCAGT TTACAAAGAA
    ACGTCTGCTC AGAATGTTGT ACAACTGGCA TTGCAAGAGT TTAATATGAC AGCAGAAGGA
    AGTCCAGAAT GGTCACTTTG TGAATGTACT GTTACGATTG ATGGAGTTAT AAAACAGAGA
    AGGTTACCAC CACAAATGGA AAATTTGGCT GAAAGAATAG CTTTGAATTC GAGATATTAT
    CTGAAAAACA ATAGTCGATC TGAACCTCTT GTACCTGATG AATTGGCTCC CGAACTTTTG
    AAAGAGGCCC AAACTCAACT GCTCAGTTTA AATGCTCAAG TCGTTGCTGC TCAACTAACT
    TTGCAAGACT TTTCAGTTTT TTCAGCAATT GAACCAACAG AATTTCTAGA TAACTTATTC
    AAGCTGGATT CCAAGTACGG CTCCCCAAAA CTTGAAGAAT TCGAACAGTT ATTCAATCGT
    GAAATGTGGT GGGTGGCGAC AGAGATTTGC ACAGAAAGAC ATGTTCAAAA ACGTGCAAAA
    CTGATTAAAA AGTTTATAAA AGTGGCGAGA TATTGTCGGG ATCTTCGAAA TTTCAATTCA
    ATGTTTGCTA TTATGAGTGG ACTTGATAAG CCAGCAGTTC GTAGGTTGCA TTCCAGTTGG
    GAACGGGTTT CGAGTAAATA CATTCGTATG CTTGATGAAA TCCATCAACT TGTCGATCCT
    TCTCGTAATA TGTCAAAGTA TCGTCAACAT CTTGCTGAAG TCGCCCAAGA ACCGCCTGTT
    GTCCCAATTT ATCCTGTAAT CAAAAAAGAT CTCACATTTG CTCACGATGG TAATGCAACA
    TATTCTGAAA AACTAATCAA TTTCGAGAAG CTTCGACTTA TTGCAAAATC AATTCGAGGT
    GTAATGAAAT TGAGTAGTGC ACCTTATGAA ATTGCTAGTA TGGCAGAGAG GAGTGGTGGA
    GTTGTAATGG ACGCTCTTCT TCATATGAAT TCATTCGAAA ATTCAAATGT AGCCACAATG
    AGGAAGGGAA TGAGTGGAAA ACAGAATCAG CCACGAAAAA AAGTTTATGA ACAAGCTCTT
    ATGGTACGGA AAGTGAAATC ATATCTCGAA GGATTACATG TTGTTGATAA TGAAATGGAA
    TTGGATTCAA TGAGTTATGA CATTGAACCA CAAGTTCAGA CAGCTCACCG TGGTGCTAAT
    TCATCATCGA CTGCAAATAT TCGTCGTGTT CCATCTCCAA CTCCATCGTC ACTTTCCTCT
    CAATCAGCCG GGTCAGCCGA TCAATCATCT CGGCATCGTC TTCTTTTCAA TGGAACCGGC
    TCAATTTCGT CAGCTGGTGG TGGTTCTAAA TTTGGAGTCG AATCTCCACA AGCTGTACAA
    AAAATGCTGT CACTTGTTCA GAATTCCAAA GTTAAAGGTG CTCCACCTCA AATCACATCT
    CCATCCACAT CAGCAAGATC CTCTCTTCAA CGGAATATGC CACGTGTAAC TGGTCGACAA
    GCGACATCCT CGGCTCAAGG GCCAGTTCAA CTGAATGAAG AGACGTCTAC GGTTACGACA
    TATTATCAAT CAGATAATGG GCGCCGGCAA CGATCTGGAT CCGAAGGAAG ATTTGATAAT
    ATTCCGCCAT CAACCTTCTA TCTAACATCC GACGGTCTTA CTGTATCACC CAGACAATCG
    TTGTCTGTTG TGATTCCAAC ACATCCTCAT GGCCACTCTC CAACATCGCC ACGGTGCCGC
    TCGCGATCAC CGGCATCTTC CGGGTGTTCT TCCTTCTCCA CCATTGCCTC AATCGCCGCT
    ACCTCGATGG CAGCCGCACC TTCGGCATTT GTCTCCAATC CGTACCAACA CCACCAAACC
    GTCCGTGGAC ATGTCATAGG ACATCGTCCA ATGCCAATCG TCACTAGTGG TTCAGCCACA
    CTCCCGAATC ATGTTTCTCC ACGAGGTTTA CCACCGAAAT CTCGTCCTAC AATCCTACCT
    GGTTCACACA CCAATTCTTC ATCAAGAATG GGTACTATCA AAGAAGCTAC ATTCCTGACA
    TCTGAACAAG TATCGCGAGT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343098
    Clone ID Related Accession (Same CDS sequence) NM_001028219.2 , NM_001028219.3
    Accession Version NM_001028219.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001028219.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGACGTGCTC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001023390.1
    CDS1..3936
    Translation

    Target ORF information:

    RefSeq Version NM_001028219.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001028219.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGACGTGCTC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343098
    Clone ID Related Accession (Same CDS sequence) NM_001028219.2 , NM_001028219.3
    Accession Version NM_001028219.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Rap guanine nucleotide exchange factor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001028219.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGACGTGCTC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001023390.1
    CDS12..3947
    Translation

    Target ORF information:

    RefSeq Version NM_001028219.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Rap guanine nucleotide exchange factor (pxf-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001028219.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATCCTC GGAAACCTCG ACAGGATCCA GTCAATGATG CTCGTTTTTA CGAATCCCTC 
    ATTAAACCAC CGCATTTAAG GACACCAGAT GATATTCGAA ATGTATACGA GCAATTGCGG
    CAGCTCGACA CCTTCAGTAA TTTGTTTATA GGTCCATTGA AAGCTCTCTG TAAAACTGCT
    CGATATGAAA GGCATCCAGC ACAATATATT CTTTTTCGGG ATGGAGATGT CGCACGAAGC
    TGGTACATTC TACTATCAGG ATCAGTTTTT ATTGAAAATC AAATCTACAT GCCTTACGGG
    TGCTTTGGCA AGCGAACAGG ACAAAATCAT CGAAGGACTC ATAATTGTCT GCTGTTACAA
    GAATCTGAAA TGATTGTGAT TGACTATCCA ACGGAACCCC AATCGAATGG AATGTCACCA
    CGAACACCTC CTCGAGGAAT TCATCATTCA GGAGAACCAG TTCATCAGAA AACTCCTAGA
    AAATCGGCAC CAAATATGTC AGTTGATTCA ATTGCAATGC CGCCACCTCC AGTTCCACCT
    CGTCCTCTAC GTCTTCCACA AACTGCAGCT AAAGGCCCAG CTCCTCTGCC GCCACGTGGT
    TTACCTAGAA CATATCCATT GGATTTTCCT GTAGATATTC CAACAACGTC ATCATCATCA
    TCAAACACTT CATATAATGA TCAACATCGT AGTCAAGTAT ATTTGAATGG ATTATCCGCT
    GATGAAGATA CTCTTGTTCG AGTGAAGCAT AGAAGAGAGA AAAGCAATTC AGTTGGAGGA
    CAAGCCCAGA ATGGAATTTC AACTGCTCGG AGGCTTCGAG GAAGAAGCAC AGCATCATCT
    ACAACTACTG AAGGTGAAAC AGCTTCCAAC GAAGGAGCAG ATTCAGACGA AGATGAAGGG
    AGTATGCCTT CACAAGAATC AAGTAGTGGA GGATTCATGG ATCTGCGAGA TTCTGTCCGA
    GAATGTCTTG AAAAGGAACC TTCAGAACGA AATTCAGAAG ATCTTGCAGT TTTACTCGAT
    TTCATGCAAC ATATGTCCGC ATTTGCCGCT TTGCCGATGT CAATAAAACG TCAGTTATGC
    TTGAAAATGG TTTTTGCGGT AGTAAACGAT GCGGGTACGG TAGTTCTTGC TCACAACGAG
    AAACTCGATT CATGGTCTGT AATAGTGAAT GGATGTGTAG AAGTTGTAAA ACCATCTGGT
    GAGCGTGTTG AATACAAGCT CGGAGATTCA TTTGGTGCAG AACCAACACC TGCAACACAA
    ATTCATATCG GTGAAATGAG AACAATGGTG GATGATTGTG AATTCGTACT TGTTGAACAT
    CGAGATTTTT GTTCAATTAT GTCGACAATT GGTGATCATA TTGAAAAAGA TCGAGACGGT
    TTGACAGGAG AAGTTGTGTC AGAAGTTGAA CGAAGGACTG TTGGAACTCA TTGTGGACAA
    GTGCTTATCA AAGGAAAACC AGACAAATTA ATACACCATT TAGTGGATGA ACGAGATCAT
    AATGTTGATC CACACTATGT CGACGATTTT CTATTGACCT ATCGAGTTTT CATAAGAGAT
    CCAACTACGA TTTTCGAGAA ATTGATGCTC TGGTTTGCCG ATTCTATATA TCGAGATAAA
    GTTGCAAGAT TGGTTCTTCT ATGGGTTAAT AATCATTTTA ATGATTTCGA GACGAACGAT
    GAGATGTGGA ATCTATTAGA ACGTTTTGAA GGAGCATTAG AAAGAGATGG AATGCATTCT
    CAACTATCTT TATTGAATAT TGCATGTTCT GTAAAAGCAA AACCTCGACA AGTGATATTA
    ACAAGGAGAA AAGATGACAA AATGATGATG AGATTAGTTG GAGGTCAAGA ATCAGGCAAC
    TCTGTATACG TGGCCGAAGT CTTCCCAGAT ACGTCAGCAG CGAGAGAAGG TGTGAAGAGA
    GCTGATGAAA TGTTAGAAGT GAATCAACAA TCAGCTAAAT ATTTATCAGC CAAAAAAGCG
    GAAGATCTAC TAACGGGGTC TCTGTCATTA ACTTTGATGC TCAAAAATAA TGTTCTTGGG
    TACAAGGAGA CAATTGGAAA AATTGAACAT AATAAGCCTA AAAATGGAAC ATCAAGATCT
    GGTGCCGGAA TTCCAATGGT AATTCCAGTT CACAAAACTT CAATAACTGG AAAGAAATCA
    TCGACGACAA GTAGCAAATC GGGTATGATG GAAAAATTGA TGACAATATT GAAATCATCA
    AAAGAAGATT CAATGGATTT CACTGATGAA GCTAAGATAT CATCAGCCGA TCTTCGTCCA
    AGTCGATCCA ATCCTGATAT CACTAGCATA TCACAGTACT ACGGACCAGT ACGAAGCGAA
    TGTCCTGAAC ACGTTCTGAA GATTTATCGA AATGATCAGA CATTCAAATA TCTGCCAGTT
    TACAAAGAAA CGTCTGCTCA GAATGTTGTA CAACTGGCAT TGCAAGAGTT TAATATGACA
    GCAGAAGGAA GTCCAGAATG GTCACTTTGT GAATGTACTG TTACGATTGA TGGAGTTATA
    AAACAGAGAA GGTTACCACC ACAAATGGAA AATTTGGCTG AAAGAATAGC TTTGAATTCG
    AGATATTATC TGAAAAACAA TAGTCGATCT GAACCTCTTG TACCTGATGA ATTGGCTCCC
    GAACTTTTGA AAGAGGCCCA AACTCAACTG CTCAGTTTAA ATGCTCAAGT CGTTGCTGCT
    CAACTAACTT TGCAAGACTT TTCAGTTTTT TCAGCAATTG AACCAACAGA ATTTCTAGAT
    AACTTATTCA AGCTGGATTC CAAGTACGGC TCCCCAAAAC TTGAAGAATT CGAACAGTTA
    TTCAATCGTG AAATGTGGTG GGTGGCGACA GAGATTTGCA CAGAAAGACA TGTTCAAAAA
    CGTGCAAAAC TGATTAAAAA GTTTATAAAA GTGGCGAGAT ATTGTCGGGA TCTTCGAAAT
    TTCAATTCAA TGTTTGCTAT TATGAGTGGA CTTGATAAGC CAGCAGTTCG TAGGTTGCAT
    TCCAGTTGGG AACGGGTTTC GAGTAAATAC ATTCGTATGC TTGATGAAAT CCATCAACTT
    GTCGATCCTT CTCGTAATAT GTCAAAGTAT CGTCAACATC TTGCTGAAGT CGCCCAAGAA
    CCGCCTGTTG TCCCAATTTA TCCTGTAATC AAAAAAGATC TCACATTTGC TCACGATGGT
    AATGCAACAT ATTCTGAAAA ACTAATCAAT TTCGAGAAGC TTCGACTTAT TGCAAAATCA
    ATTCGAGGTG TAATGAAATT GAGTAGTGCA CCTTATGAAA TTGCTAGTAT GGCAGAGAGG
    AGTGGTGGAG TTGTAATGGA CGCTCTTCTT CATATGAATT CATTCGAAAA TTCAAATGTA
    GCCACAATGA GGAAGGGAAT GAGTGGAAAA CAGAATCAGC CACGAAAAAA AGTTTATGAA
    CAAGCTCTTA TGGTACGGAA AGTGAAATCA TATCTCGAAG GATTACATGT TGTTGATAAT
    GAAATGGAAT TGGATTCAAT GAGTTATGAC ATTGAACCAC AAGTTCAGAC AGCTCACCGT
    GGTGCTAATT CATCATCGAC TGCAAATATT CGTCGTGTTC CATCTCCAAC TCCATCGTCA
    CTTTCCTCTC AATCAGCCGG GTCAGCCGAT CAATCATCTC GGCATCGTCT TCTTTTCAAT
    GGAACCGGCT CAATTTCGTC AGCTGGTGGT GGTTCTAAAT TTGGAGTCGA ATCTCCACAA
    GCTGTACAAA AAATGCTGTC ACTTGTTCAG AATTCCAAAG TTAAAGGTGC TCCACCTCAA
    ATCACATCTC CATCCACATC AGCAAGATCC TCTCTTCAAC GGAATATGCC ACGTGTAACT
    GGTCGACAAG CGACATCCTC GGCTCAAGGG CCAGTTCAAC TGAATGAAGA GACGTCTACG
    GTTACGACAT ATTATCAATC AGACGTGCTC ATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.