mca-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	mca-1
Entrez Gene ID	177795
Full Name	Calcium-transporting ATPase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001028255.2	NP_001023426.1	Calcium-transporting ATPase
NM_001268480.1	NP_001255409.1	Calcium-transporting ATPase
NM_001028256.4	NP_001023427.1	Calcium-transporting ATPase
NM_069308.5	NP_501709.2	Calcium-transporting ATPase
NM_069308.6	NP_501709.2	Calcium-transporting ATPase
NM_001028255.3	NP_001023426.1	Calcium-transporting ATPase
NM_001028256.5	NP_001023427.1	Calcium-transporting ATPase
NM_001268480.2	NP_001255409.1	Calcium-transporting ATPase

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	mca-1	NP_001023427.1
Arabidopsis thaliana (thale cress)	ACA1	NP_849716.1
Oryza sativa (rice)	Os12g0586600	NP_001067159.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mca-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mca-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342197	NM_069308.6 Latest version!	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa343223	NM_001028255.3 Latest version!	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa343609	NM_001268480.2 Latest version!	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa344389	NM_001028256.5 Latest version!	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa342197	NM_069308.5	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa343223	NM_001028255.2	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa344389	NM_001028256.4	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa343609	NM_001268480.1	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mca-1 ( NM_069308.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501709.2 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Price & Availability↑

CloneID	OCa342197
Clone ID Related Accession (Same CDS sequence)	NM_069308.5 , NM_069308.6
Accession Version	NM_069308.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069308.4. COMPLETENESS: incomplete on both ends.

ATGCAAAAGA GCCAAAATGT GACCGCAGTA ACGGAAACAA ATGGTGTGGC GGCAGCACTG 
GGCGGCCACC ACACCTCTCC TGATACTAAC GCAGGAAAGA CCAAGGATGC AAAAGAATTT 
GGCTGCAGTC TTGGCGACCT TCGCGGACTC ATGGAGGCTC GCGGAGCTGA GGCGATAGTT 
CGGCTGTCCA CAGAACACGA AGGCGTCGAG GGATTGTGCA AGAAATTGAA GACTGACTCG 
TTGGTTGGTC TCAATGGTGA ACAAGCAGAT CTCGATCGAC GACGACATGT CTACGGAGCC 
AATACTATTC CACCAGCCAA AAGTAAAGGA TTCGTGCGGC TCGTTTTGGA TGCATGCAAG 
GACCCGACTC TTGTTATCCT GGTACTTTCC GGCTTCATCA ATTTAGCTCT ATCATTCTAC 
GAACCGACAT CGGCGGCCGA AGACGCAACT CAACACCTTG TCAACGCGAC GACGGCGGCA 
ATTCTTGCCA ATGGAACCTT TATGTCAACC ACCGAAGCAC CATCTGAAGG TCATGGAACT 
GCATGGATTG AAGGAGTTGC TATTCTTCTC TGTGTTATTG TCGTCGTGCT GGTCACCGCT 
GTCAACGATT ATTCGAAAGA ACGACAGTTT AGAAGTTTGC AGGAAAAGAT TGAAACTGGC 
CAAAAGTTCT CTGTTATTCG TAATGGGGAG GCTATTGATG TCCCCGTCTC TGATCTGGTA 
GTCGGTGACA TTGCACGTGT CAAATATGGT GATCTTCTTC CTGCCGATGG ATTTTTGATT 
CAGAGTAATG ATTTGAAGAT CGATGAATCA TCCCTCACTG GAGAATCAGA TCACATCAAA 
AAGAGCATTG AATCCGATCC AGTTCTCCTT TCGGGAACAT ATGCTATGGA AGGTTCCGGA 
AAAATGCTCA TCACTGCCGT AGGAGTCAAC TCACAAACTG GTATTATCAT GACTCTTCTC 
GGGGCTGGAA AAGCTGGAAT CGGAGATGAT GACTCTACAT CAACATCTTC AAGTTCTTCT 
TCATCATCTA GTTCATCCGG AAGTTCGTCT AACGGAAGCT CTGACAGCTC AAAATCTGGC 
GATGACGACT TGACAGCCAA ATCAGTTCTG CAAGCTAAAC TTTCCAAGTT GGCCCTTCAA 
ATCATCTATT GTGGAACTAC AATTGCCATC ATTGCACTTA TCGTCTTGGT CACTCGATTC 
TGTCTTGACC ACTATGTTTT TGAAAAGAAC GAGTTCTCAC TGGTCGATAT TCAAATGTTC 
GTCAAGTTCT TCATTATTGC TGTTACCATC TTGGTCATTT CCATACCTGA AGGTCTTCCA 
TTAGCTATTG CTCTTGCTCT TACTTACTCT GTCAGAAAAA TGATGCACGA TAACAACTTG 
GTCCGTCACT TGGATGCATG TGAGACTATG GGAAATGCTA CTTCAATTTG CTCTGACAAA 
ACTGGAACTC TCACTACCAA CCGAATGACT GTTGTTCAAT CATACATCAA CGGAAACCAT 
TACACCAGTC AAGAAGCTCA ACCACATGGA GCCAACCTTC CAGGAAGCAC TGGCCCAATT 
CTTATGGAGG CTATCTCAGT CAACTGTGCC TATAACTCAA TGATTGTGGA GCCAACAAAA 
GCTGGAGAGC AAATTCAGCA ACTTGGAAAC AAGACCGAAT GTGGTCTTCT AGGATTTGTA 
AACCGTCTTG GAGGTGACTA TGCTGCCATC AGAAAGAAGT TCCCAGAACA TGACCTCACC 
AAGGTCTACA CTTTCAACTC TTCTCGTAAA TGCATGATGA CTGTTGTGCC ATATGCCGAG 
AATGGCCAGA ATATCGGATA CCGTGTCTAT TGCAAAGGAG CCTCTGAGAT TGTGCTCGGC 
AGATGTACAT ACTTGATCGG TTCTGACGGA AAGCCACATC AGCTTACTGG TGATCGATTG 
AAGGAGATTA CATCTACTAT CATTCATGAG ATGGCTAACA GTGGTCTGAG AACTATCTGT 
GTTGCTTATA AGACAATCAT CAAGAAGGGA ACAAGAGATG TGGAGAAGAC TGAGATCGAA 
TTCGCCGAAG ACTCTGATAT CGATTGGGAT GACGAGGATG CCATGTACCA AAACTTCACC 
GGAATTGCTA TTTGCGGAAT TCAAGATCCA GTTCGACCAG AGGTACCAGT CGCAATTTCA 
AAATGCAAAA AAGCTGGAAT CACTGTAAGA ATGGTTACTG GTGATAACAT CATGACCGCC 
AGAGCAATTG CAATGTCCTG TAAAATCTTG GAACCCGGAG AGGACTTTCT GGCTCTTGAA 
GGAAAGGAAT TCAATGAGAG AATCCGCGAC GAAAATGGAA AAGTCTCTCA GGCAAAACTT 
GATGAAATCT GGCCACGTCT TCGTGTGTTG GCTCGTGCTC AACCTGCTGA TAAGTACACT 
CTTGTTAAGG GAATTATCGA CTCAAAAGCT ACTCCACAAA GAGAAATTGT TGCTGTAACT 
GGAGATGGTA CAAATGATGG TCCAGCTTTG AAGAAGGCTG ATGTTGGATT CGCTATGGGA 
ATTGCTGGAA CTGACGTTGC AAAGGAAGCT TCTGATATTA TTTTGACTGA TGATAATTTC 
ACTTCAATTG TTAAAGCAGT TATGTGGGGA AGAAATGTGT ACGATTCCAT CTCCAAGTTC 
TTGCAATTCC AACTCACTGT CAATGTCGTC GCTGTCATCA CTGCCTTCGT TGGAGCTGTC 
ACAGTGTCTG ATTCACCATT GAAGGCAGTT CACATGCTTT GGATTAATCT TATCATGGAC 
ACTCTTGCTT CATTGGCCCT CGCAACTGAA CAGCCAACTG ATGAACTACT CGAAAGAAAA 
CCATACGGTC GCAAGAAGTC GCTCATCTCA AGAACAATGG TCAAGAATAT TCTTTGTCAT 
GCTCTTTACC AACTTATCAT TATCTTTGTC ATCTTCTTCT ACGGAGACAC CATCTTTGGA 
ATCAAGACAG GACTCTATGC ACCACTCTTC GCTCCACCAT CACAACATTT CACTTTGGTT 
TTCAATGCAT TCGTTATGAT GACTGTCTTC AATGAGATCA ATGCTCGTAA GGTTCACGGA 
GAGCGTAACG TGTTTAAAGG ATTGGCATCT AACCGAGTTT TCTGTGTCAT TTGGGTTACA 
ACATTCATTG CTCAAATCAT TATTGTTCAA TTCGGAGGTG CCTGGTTCTC AACTGCTCCA 
CTTACGCTTC AACAATGGAT TGTATGCCTC GTTCTTGGAT TCTCAACTCT TATCTGGGGA 
CAAATTGTGG CAACTATCCC ATCAAAGAAG CTACCAAAGG CCTGGAAAGT TGGAAAGGGA 
GAAGTTCAAC CAGCCAATCT TCACATCAAT GGAGACTACA ATGTCCGTGC TCGTTCCCGT 
GCGGTTACTC TTCGCAGATC TGGAAAGTCA CTCTGGGTTA GAGGAATGTT CATTATTGGA 
AACCATCTCC GCGTTCTTCG TGCTTTCGGA ATGGAAAAGA GTGAAAAGGC TGCCTTCGGA 
CGTACTGCTC CAGCAATGAC GGCTGAAGCT GCTGAACGCT GGCGTGCCAG TTACCGCAAG 
TACCGTCACC AGAAACATCA AGAGAAGAAA GCCACCGCGG AAACTGCTGA ATCTGTGAAA 
TCTGCCGACT GGGCCAAGGA ACAGAAGGAA AAGAAGAAGA CGTTCAAACA GATCAAACAG 
GTTGCACGTG GAAAGAGTCT TGACAAGGAT TCTAAGAAGC ATCACAAGAA GAGGAAGGAT 
CAGACAAATG TCGATATGGA AGACATTGAA CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501709.2
CDS	1..3759
Translation	MQKSQNVTAVTETNGVAAALGGHHTSPDTNAGKTKDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_069308.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069308.5

ORF Insert Sequence:

ATGCAAAAGA GCCAAAATGT GACCGCAGTA ACGGAAACAA ATGGTGTGGC GGCAGCACTG 
GGCGGCCACC ACACCTCTCC TGATACTAAC GCAGGAAAGA CCAAGGATGC AAAAGAATTT 
GGCTGCAGTC TTGGCGACCT TCGCGGACTC ATGGAGGCTC GCGGAGCTGA GGCGATAGTT 
CGGCTGTCCA CAGAACACGA AGGCGTCGAG GGATTGTGCA AGAAATTGAA GACTGACTCG 
TTGGTTGGTC TCAATGGTGA ACAAGCAGAT CTCGATCGAC GACGACATGT CTACGGAGCC 
AATACTATTC CACCAGCCAA AAGTAAAGGA TTCGTGCGGC TCGTTTTGGA TGCATGCAAG 
GACCCGACTC TTGTTATCCT GGTACTTTCC GGCTTCATCA ATTTAGCTCT ATCATTCTAC 
GAACCGACAT CGGCGGCCGA AGACGCAACT CAACACCTTG TCAACGCGAC GACGGCGGCA 
ATTCTTGCCA ATGGAACCTT TATGTCAACC ACCGAAGCAC CATCTGAAGG TCATGGAACT 
GCATGGATTG AAGGAGTTGC TATTCTTCTC TGTGTTATTG TCGTCGTGCT GGTCACCGCT 
GTCAACGATT ATTCGAAAGA ACGACAGTTT AGAAGTTTGC AGGAAAAGAT TGAAACTGGC 
CAAAAGTTCT CTGTTATTCG TAATGGGGAG GCTATTGATG TCCCCGTCTC TGATCTGGTA 
GTCGGTGACA TTGCACGTGT CAAATATGGT GATCTTCTTC CTGCCGATGG ATTTTTGATT 
CAGAGTAATG ATTTGAAGAT CGATGAATCA TCCCTCACTG GAGAATCAGA TCACATCAAA 
AAGAGCATTG AATCCGATCC AGTTCTCCTT TCGGGAACAT ATGCTATGGA AGGTTCCGGA 
AAAATGCTCA TCACTGCCGT AGGAGTCAAC TCACAAACTG GTATTATCAT GACTCTTCTC 
GGGGCTGGAA AAGCTGGAAT CGGAGATGAT GACTCTACAT CAACATCTTC AAGTTCTTCT 
TCATCATCTA GTTCATCCGG AAGTTCGTCT AACGGAAGCT CTGACAGCTC AAAATCTGGC 
GATGACGACT TGACAGCCAA ATCAGTTCTG CAAGCTAAAC TTTCCAAGTT GGCCCTTCAA 
ATCATCTATT GTGGAACTAC AATTGCCATC ATTGCACTTA TCGTCTTGGT CACTCGATTC 
TGTCTTGACC ACTATGTTTT TGAAAAGAAC GAGTTCTCAC TGGTCGATAT TCAAATGTTC 
GTCAAGTTCT TCATTATTGC TGTTACCATC TTGGTCATTT CCATACCTGA AGGTCTTCCA 
TTAGCTATTG CTCTTGCTCT TACTTACTCT GTCAGAAAAA TGATGCACGA TAACAACTTG 
GTCCGTCACT TGGATGCATG TGAGACTATG GGAAATGCTA CTTCAATTTG CTCTGACAAA 
ACTGGAACTC TCACTACCAA CCGAATGACT GTTGTTCAAT CATACATCAA CGGAAACCAT 
TACACCAGTC AAGAAGCTCA ACCACATGGA GCCAACCTTC CAGGAAGCAC TGGCCCAATT 
CTTATGGAGG CTATCTCAGT CAACTGTGCC TATAACTCAA TGATTGTGGA GCCAACAAAA 
GCTGGAGAGC AAATTCAGCA ACTTGGAAAC AAGACCGAAT GTGGTCTTCT AGGATTTGTA 
AACCGTCTTG GAGGTGACTA TGCTGCCATC AGAAAGAAGT TCCCAGAACA TGACCTCACC 
AAGGTCTACA CTTTCAACTC TTCTCGTAAA TGCATGATGA CTGTTGTGCC ATATGCCGAG 
AATGGCCAGA ATATCGGATA CCGTGTCTAT TGCAAAGGAG CCTCTGAGAT TGTGCTCGGC 
AGATGTACAT ACTTGATCGG TTCTGACGGA AAGCCACATC AGCTTACTGG TGATCGATTG 
AAGGAGATTA CATCTACTAT CATTCATGAG ATGGCTAACA GTGGTCTGAG AACTATCTGT 
GTTGCTTATA AGACAATCAT CAAGAAGGGA ACAAGAGATG TGGAGAAGAC TGAGATCGAA 
TTCGCCGAAG ACTCTGATAT CGATTGGGAT GACGAGGATG CCATGTACCA AAACTTCACC 
GGAATTGCTA TTTGCGGAAT TCAAGATCCA GTTCGACCAG AGGTACCAGT CGCAATTTCA 
AAATGCAAAA AAGCTGGAAT CACTGTAAGA ATGGTTACTG GTGATAACAT CATGACCGCC 
AGAGCAATTG CAATGTCCTG TAAAATCTTG GAACCCGGAG AGGACTTTCT GGCTCTTGAA 
GGAAAGGAAT TCAATGAGAG AATCCGCGAC GAAAATGGAA AAGTCTCTCA GGCAAAACTT 
GATGAAATCT GGCCACGTCT TCGTGTGTTG GCTCGTGCTC AACCTGCTGA TAAGTACACT 
CTTGTTAAGG GAATTATCGA CTCAAAAGCT ACTCCACAAA GAGAAATTGT TGCTGTAACT 
GGAGATGGTA CAAATGATGG TCCAGCTTTG AAGAAGGCTG ATGTTGGATT CGCTATGGGA 
ATTGCTGGAA CTGACGTTGC AAAGGAAGCT TCTGATATTA TTTTGACTGA TGATAATTTC 
ACTTCAATTG TTAAAGCAGT TATGTGGGGA AGAAATGTGT ACGATTCCAT CTCCAAGTTC 
TTGCAATTCC AACTCACTGT CAATGTCGTC GCTGTCATCA CTGCCTTCGT TGGAGCTGTC 
ACAGTGTCTG ATTCACCATT GAAGGCAGTT CACATGCTTT GGATTAATCT TATCATGGAC 
ACTCTTGCTT CATTGGCCCT CGCAACTGAA CAGCCAACTG ATGAACTACT CGAAAGAAAA 
CCATACGGTC GCAAGAAGTC GCTCATCTCA AGAACAATGG TCAAGAATAT TCTTTGTCAT 
GCTCTTTACC AACTTATCAT TATCTTTGTC ATCTTCTTCT ACGGAGACAC CATCTTTGGA 
ATCAAGACAG GACTCTATGC ACCACTCTTC GCTCCACCAT CACAACATTT CACTTTGGTT 
TTCAATGCAT TCGTTATGAT GACTGTCTTC AATGAGATCA ATGCTCGTAA GGTTCACGGA 
GAGCGTAACG TGTTTAAAGG ATTGGCATCT AACCGAGTTT TCTGTGTCAT TTGGGTTACA 
ACATTCATTG CTCAAATCAT TATTGTTCAA TTCGGAGGTG CCTGGTTCTC AACTGCTCCA 
CTTACGCTTC AACAATGGAT TGTATGCCTC GTTCTTGGAT TCTCAACTCT TATCTGGGGA 
CAAATTGTGG CAACTATCCC ATCAAAGAAG CTACCAAAGG CCTGGAAAGT TGGAAAGGGA 
GAAGTTCAAC CAGCCAATCT TCACATCAAT GGAGACTACA ATGTCCGTGC TCGTTCCCGT 
GCGGTTACTC TTCGCAGATC TGGAAAGTCA CTCTGGGTTA GAGGAATGTT CATTATTGGA 
AACCATCTCC GCGTTCTTCG TGCTTTCGGA ATGGAAAAGA GTGAAAAGGC TGCCTTCGGA 
CGTACTGCTC CAGCAATGAC GGCTGAAGCT GCTGAACGCT GGCGTGCCAG TTACCGCAAG 
TACCGTCACC AGAAACATCA AGAGAAGAAA GCCACCGCGG AAACTGCTGA ATCTGTGAAA 
TCTGCCGACT GGGCCAAGGA ACAGAAGGAA AAGAAGAAGA CGTTCAAACA GATCAAACAG 
GTTGCACGTG GAAAGAGTCT TGACAAGGAT TCTAAGAAGC ATCACAAGAA GAGGAAGGAT 
CAGACAAATG TCGATATGGA AGACATTGAA CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_069308.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501709.2 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

Price & Availability↑

CloneID	OCa342197
Clone ID Related Accession (Same CDS sequence)	NM_069308.5 , NM_069308.6
Accession Version	NM_069308.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069308.5.

ATGCAAAAGA GCCAAAATGT GACCGCAGTA ACGGAAACAA ATGGTGTGGC GGCAGCACTG 
GGCGGCCACC ACACCTCTCC TGATACTAAC GCAGGAAAGA CCAAGGATGC AAAAGAATTT 
GGCTGCAGTC TTGGCGACCT TCGCGGACTC ATGGAGGCTC GCGGAGCTGA GGCGATAGTT 
CGGCTGTCCA CAGAACACGA AGGCGTCGAG GGATTGTGCA AGAAATTGAA GACTGACTCG 
TTGGTTGGTC TCAATGGTGA ACAAGCAGAT CTCGATCGAC GACGACATGT CTACGGAGCC 
AATACTATTC CACCAGCCAA AAGTAAAGGA TTCGTGCGGC TCGTTTTGGA TGCATGCAAG 
GACCCGACTC TTGTTATCCT GGTACTTTCC GGCTTCATCA ATTTAGCTCT ATCATTCTAC 
GAACCGACAT CGGCGGCCGA AGACGCAACT CAACACCTTG TCAACGCGAC GACGGCGGCA 
ATTCTTGCCA ATGGAACCTT TATGTCAACC ACCGAAGCAC CATCTGAAGG TCATGGAACT 
GCATGGATTG AAGGAGTTGC TATTCTTCTC TGTGTTATTG TCGTCGTGCT GGTCACCGCT 
GTCAACGATT ATTCGAAAGA ACGACAGTTT AGAAGTTTGC AGGAAAAGAT TGAAACTGGC 
CAAAAGTTCT CTGTTATTCG TAATGGGGAG GCTATTGATG TCCCCGTCTC TGATCTGGTA 
GTCGGTGACA TTGCACGTGT CAAATATGGT GATCTTCTTC CTGCCGATGG ATTTTTGATT 
CAGAGTAATG ATTTGAAGAT CGATGAATCA TCCCTCACTG GAGAATCAGA TCACATCAAA 
AAGAGCATTG AATCCGATCC AGTTCTCCTT TCGGGAACAT ATGCTATGGA AGGTTCCGGA 
AAAATGCTCA TCACTGCCGT AGGAGTCAAC TCACAAACTG GTATTATCAT GACTCTTCTC 
GGGGCTGGAA AAGCTGGAAT CGGAGATGAT GACTCTACAT CAACATCTTC AAGTTCTTCT 
TCATCATCTA GTTCATCCGG AAGTTCGTCT AACGGAAGCT CTGACAGCTC AAAATCTGGC 
GATGACGACT TGACAGCCAA ATCAGTTCTG CAAGCTAAAC TTTCCAAGTT GGCCCTTCAA 
ATCATCTATT GTGGAACTAC AATTGCCATC ATTGCACTTA TCGTCTTGGT CACTCGATTC 
TGTCTTGACC ACTATGTTTT TGAAAAGAAC GAGTTCTCAC TGGTCGATAT TCAAATGTTC 
GTCAAGTTCT TCATTATTGC TGTTACCATC TTGGTCATTT CCATACCTGA AGGTCTTCCA 
TTAGCTATTG CTCTTGCTCT TACTTACTCT GTCAGAAAAA TGATGCACGA TAACAACTTG 
GTCCGTCACT TGGATGCATG TGAGACTATG GGAAATGCTA CTTCAATTTG CTCTGACAAA 
ACTGGAACTC TCACTACCAA CCGAATGACT GTTGTTCAAT CATACATCAA CGGAAACCAT 
TACACCAGTC AAGAAGCTCA ACCACATGGA GCCAACCTTC CAGGAAGCAC TGGCCCAATT 
CTTATGGAGG CTATCTCAGT CAACTGTGCC TATAACTCAA TGATTGTGGA GCCAACAAAA 
GCTGGAGAGC AAATTCAGCA ACTTGGAAAC AAGACCGAAT GTGGTCTTCT AGGATTTGTA 
AACCGTCTTG GAGGTGACTA TGCTGCCATC AGAAAGAAGT TCCCAGAACA TGACCTCACC 
AAGGTCTACA CTTTCAACTC TTCTCGTAAA TGCATGATGA CTGTTGTGCC ATATGCCGAG 
AATGGCCAGA ATATCGGATA CCGTGTCTAT TGCAAAGGAG CCTCTGAGAT TGTGCTCGGC 
AGATGTACAT ACTTGATCGG TTCTGACGGA AAGCCACATC AGCTTACTGG TGATCGATTG 
AAGGAGATTA CATCTACTAT CATTCATGAG ATGGCTAACA GTGGTCTGAG AACTATCTGT 
GTTGCTTATA AGACAATCAT CAAGAAGGGA ACAAGAGATG TGGAGAAGAC TGAGATCGAA 
TTCGCCGAAG ACTCTGATAT CGATTGGGAT GACGAGGATG CCATGTACCA AAACTTCACC 
GGAATTGCTA TTTGCGGAAT TCAAGATCCA GTTCGACCAG AGGTACCAGT CGCAATTTCA 
AAATGCAAAA AAGCTGGAAT CACTGTAAGA ATGGTTACTG GTGATAACAT CATGACCGCC 
AGAGCAATTG CAATGTCCTG TAAAATCTTG GAACCCGGAG AGGACTTTCT GGCTCTTGAA 
GGAAAGGAAT TCAATGAGAG AATCCGCGAC GAAAATGGAA AAGTCTCTCA GGCAAAACTT 
GATGAAATCT GGCCACGTCT TCGTGTGTTG GCTCGTGCTC AACCTGCTGA TAAGTACACT 
CTTGTTAAGG GAATTATCGA CTCAAAAGCT ACTCCACAAA GAGAAATTGT TGCTGTAACT 
GGAGATGGTA CAAATGATGG TCCAGCTTTG AAGAAGGCTG ATGTTGGATT CGCTATGGGA 
ATTGCTGGAA CTGACGTTGC AAAGGAAGCT TCTGATATTA TTTTGACTGA TGATAATTTC 
ACTTCAATTG TTAAAGCAGT TATGTGGGGA AGAAATGTGT ACGATTCCAT CTCCAAGTTC 
TTGCAATTCC AACTCACTGT CAATGTCGTC GCTGTCATCA CTGCCTTCGT TGGAGCTGTC 
ACAGTGTCTG ATTCACCATT GAAGGCAGTT CACATGCTTT GGATTAATCT TATCATGGAC 
ACTCTTGCTT CATTGGCCCT CGCAACTGAA CAGCCAACTG ATGAACTACT CGAAAGAAAA 
CCATACGGTC GCAAGAAGTC GCTCATCTCA AGAACAATGG TCAAGAATAT TCTTTGTCAT 
GCTCTTTACC AACTTATCAT TATCTTTGTC ATCTTCTTCT ACGGAGACAC CATCTTTGGA 
ATCAAGACAG GACTCTATGC ACCACTCTTC GCTCCACCAT CACAACATTT CACTTTGGTT 
TTCAATGCAT TCGTTATGAT GACTGTCTTC AATGAGATCA ATGCTCGTAA GGTTCACGGA 
GAGCGTAACG TGTTTAAAGG ATTGGCATCT AACCGAGTTT TCTGTGTCAT TTGGGTTACA 
ACATTCATTG CTCAAATCAT TATTGTTCAA TTCGGAGGTG CCTGGTTCTC AACTGCTCCA 
CTTACGCTTC AACAATGGAT TGTATGCCTC GTTCTTGGAT TCTCAACTCT TATCTGGGGA 
CAAATTGTGG CAACTATCCC ATCAAAGAAG CTACCAAAGG CCTGGAAAGT TGGAAAGGGA 
GAAGTTCAAC CAGCCAATCT TCACATCAAT GGAGACTACA ATGTCCGTGC TCGTTCCCGT 
GCGGTTACTC TTCGCAGATC TGGAAAGTCA CTCTGGGTTA GAGGAATGTT CATTATTGGA 
AACCATCTCC GCGTTCTTCG TGCTTTCGGA ATGGAAAAGA GTGAAAAGGC TGCCTTCGGA 
CGTACTGCTC CAGCAATGAC GGCTGAAGCT GCTGAACGCT GGCGTGCCAG TTACCGCAAG 
TACCGTCACC AGAAACATCA AGAGAAGAAA GCCACCGCGG AAACTGCTGA ATCTGTGAAA 
TCTGCCGACT GGGCCAAGGA ACAGAAGGAA AAGAAGAAGA CGTTCAAACA GATCAAACAG 
GTTGCACGTG GAAAGAGTCT TGACAAGGAT TCTAAGAAGC ATCACAAGAA GAGGAAGGAT 
CAGACAAATG TCGATATGGA AGACATTGAA CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501709.2
CDS	80..3838
Translation	MQKSQNVTAVTETNGVAAALGGHHTSPDTNAGKTKDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_069308.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069308.6

ORF Insert Sequence:

ATGCAAAAGA GCCAAAATGT GACCGCAGTA ACGGAAACAA ATGGTGTGGC GGCAGCACTG 
GGCGGCCACC ACACCTCTCC TGATACTAAC GCAGGAAAGA CCAAGGATGC AAAAGAATTT 
GGCTGCAGTC TTGGCGACCT TCGCGGACTC ATGGAGGCTC GCGGAGCTGA GGCGATAGTT 
CGGCTGTCCA CAGAACACGA AGGCGTCGAG GGATTGTGCA AGAAATTGAA GACTGACTCG 
TTGGTTGGTC TCAATGGTGA ACAAGCAGAT CTCGATCGAC GACGACATGT CTACGGAGCC 
AATACTATTC CACCAGCCAA AAGTAAAGGA TTCGTGCGGC TCGTTTTGGA TGCATGCAAG 
GACCCGACTC TTGTTATCCT GGTACTTTCC GGCTTCATCA ATTTAGCTCT ATCATTCTAC 
GAACCGACAT CGGCGGCCGA AGACGCAACT CAACACCTTG TCAACGCGAC GACGGCGGCA 
ATTCTTGCCA ATGGAACCTT TATGTCAACC ACCGAAGCAC CATCTGAAGG TCATGGAACT 
GCATGGATTG AAGGAGTTGC TATTCTTCTC TGTGTTATTG TCGTCGTGCT GGTCACCGCT 
GTCAACGATT ATTCGAAAGA ACGACAGTTT AGAAGTTTGC AGGAAAAGAT TGAAACTGGC 
CAAAAGTTCT CTGTTATTCG TAATGGGGAG GCTATTGATG TCCCCGTCTC TGATCTGGTA 
GTCGGTGACA TTGCACGTGT CAAATATGGT GATCTTCTTC CTGCCGATGG ATTTTTGATT 
CAGAGTAATG ATTTGAAGAT CGATGAATCA TCCCTCACTG GAGAATCAGA TCACATCAAA 
AAGAGCATTG AATCCGATCC AGTTCTCCTT TCGGGAACAT ATGCTATGGA AGGTTCCGGA 
AAAATGCTCA TCACTGCCGT AGGAGTCAAC TCACAAACTG GTATTATCAT GACTCTTCTC 
GGGGCTGGAA AAGCTGGAAT CGGAGATGAT GACTCTACAT CAACATCTTC AAGTTCTTCT 
TCATCATCTA GTTCATCCGG AAGTTCGTCT AACGGAAGCT CTGACAGCTC AAAATCTGGC 
GATGACGACT TGACAGCCAA ATCAGTTCTG CAAGCTAAAC TTTCCAAGTT GGCCCTTCAA 
ATCATCTATT GTGGAACTAC AATTGCCATC ATTGCACTTA TCGTCTTGGT CACTCGATTC 
TGTCTTGACC ACTATGTTTT TGAAAAGAAC GAGTTCTCAC TGGTCGATAT TCAAATGTTC 
GTCAAGTTCT TCATTATTGC TGTTACCATC TTGGTCATTT CCATACCTGA AGGTCTTCCA 
TTAGCTATTG CTCTTGCTCT TACTTACTCT GTCAGAAAAA TGATGCACGA TAACAACTTG 
GTCCGTCACT TGGATGCATG TGAGACTATG GGAAATGCTA CTTCAATTTG CTCTGACAAA 
ACTGGAACTC TCACTACCAA CCGAATGACT GTTGTTCAAT CATACATCAA CGGAAACCAT 
TACACCAGTC AAGAAGCTCA ACCACATGGA GCCAACCTTC CAGGAAGCAC TGGCCCAATT 
CTTATGGAGG CTATCTCAGT CAACTGTGCC TATAACTCAA TGATTGTGGA GCCAACAAAA 
GCTGGAGAGC AAATTCAGCA ACTTGGAAAC AAGACCGAAT GTGGTCTTCT AGGATTTGTA 
AACCGTCTTG GAGGTGACTA TGCTGCCATC AGAAAGAAGT TCCCAGAACA TGACCTCACC 
AAGGTCTACA CTTTCAACTC TTCTCGTAAA TGCATGATGA CTGTTGTGCC ATATGCCGAG 
AATGGCCAGA ATATCGGATA CCGTGTCTAT TGCAAAGGAG CCTCTGAGAT TGTGCTCGGC 
AGATGTACAT ACTTGATCGG TTCTGACGGA AAGCCACATC AGCTTACTGG TGATCGATTG 
AAGGAGATTA CATCTACTAT CATTCATGAG ATGGCTAACA GTGGTCTGAG AACTATCTGT 
GTTGCTTATA AGACAATCAT CAAGAAGGGA ACAAGAGATG TGGAGAAGAC TGAGATCGAA 
TTCGCCGAAG ACTCTGATAT CGATTGGGAT GACGAGGATG CCATGTACCA AAACTTCACC 
GGAATTGCTA TTTGCGGAAT TCAAGATCCA GTTCGACCAG AGGTACCAGT CGCAATTTCA 
AAATGCAAAA AAGCTGGAAT CACTGTAAGA ATGGTTACTG GTGATAACAT CATGACCGCC 
AGAGCAATTG CAATGTCCTG TAAAATCTTG GAACCCGGAG AGGACTTTCT GGCTCTTGAA 
GGAAAGGAAT TCAATGAGAG AATCCGCGAC GAAAATGGAA AAGTCTCTCA GGCAAAACTT 
GATGAAATCT GGCCACGTCT TCGTGTGTTG GCTCGTGCTC AACCTGCTGA TAAGTACACT 
CTTGTTAAGG GAATTATCGA CTCAAAAGCT ACTCCACAAA GAGAAATTGT TGCTGTAACT 
GGAGATGGTA CAAATGATGG TCCAGCTTTG AAGAAGGCTG ATGTTGGATT CGCTATGGGA 
ATTGCTGGAA CTGACGTTGC AAAGGAAGCT TCTGATATTA TTTTGACTGA TGATAATTTC 
ACTTCAATTG TTAAAGCAGT TATGTGGGGA AGAAATGTGT ACGATTCCAT CTCCAAGTTC 
TTGCAATTCC AACTCACTGT CAATGTCGTC GCTGTCATCA CTGCCTTCGT TGGAGCTGTC 
ACAGTGTCTG ATTCACCATT GAAGGCAGTT CACATGCTTT GGATTAATCT TATCATGGAC 
ACTCTTGCTT CATTGGCCCT CGCAACTGAA CAGCCAACTG ATGAACTACT CGAAAGAAAA 
CCATACGGTC GCAAGAAGTC GCTCATCTCA AGAACAATGG TCAAGAATAT TCTTTGTCAT 
GCTCTTTACC AACTTATCAT TATCTTTGTC ATCTTCTTCT ACGGAGACAC CATCTTTGGA 
ATCAAGACAG GACTCTATGC ACCACTCTTC GCTCCACCAT CACAACATTT CACTTTGGTT 
TTCAATGCAT TCGTTATGAT GACTGTCTTC AATGAGATCA ATGCTCGTAA GGTTCACGGA 
GAGCGTAACG TGTTTAAAGG ATTGGCATCT AACCGAGTTT TCTGTGTCAT TTGGGTTACA 
ACATTCATTG CTCAAATCAT TATTGTTCAA TTCGGAGGTG CCTGGTTCTC AACTGCTCCA 
CTTACGCTTC AACAATGGAT TGTATGCCTC GTTCTTGGAT TCTCAACTCT TATCTGGGGA 
CAAATTGTGG CAACTATCCC ATCAAAGAAG CTACCAAAGG CCTGGAAAGT TGGAAAGGGA 
GAAGTTCAAC CAGCCAATCT TCACATCAAT GGAGACTACA ATGTCCGTGC TCGTTCCCGT 
GCGGTTACTC TTCGCAGATC TGGAAAGTCA CTCTGGGTTA GAGGAATGTT CATTATTGGA 
AACCATCTCC GCGTTCTTCG TGCTTTCGGA ATGGAAAAGA GTGAAAAGGC TGCCTTCGGA 
CGTACTGCTC CAGCAATGAC GGCTGAAGCT GCTGAACGCT GGCGTGCCAG TTACCGCAAG 
TACCGTCACC AGAAACATCA AGAGAAGAAA GCCACCGCGG AAACTGCTGA ATCTGTGAAA 
TCTGCCGACT GGGCCAAGGA ACAGAAGGAA AAGAAGAAGA CGTTCAAACA GATCAAACAG 
GTTGCACGTG GAAAGAGTCT TGACAAGGAT TCTAAGAAGC ATCACAAGAA GAGGAAGGAT 
CAGACAAATG TCGATATGGA AGACATTGAA CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001028255.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023426.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

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CloneID	OCa343223
Clone ID Related Accession (Same CDS sequence)	NM_001028255.2 , NM_001028255.3
Accession Version	NM_001028255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001028255.1. COMPLETENESS: incomplete on both ends.

ATGAGATTGA GAAGAGGTCA TACCTACGAT GAGGATGCAA AAGAATTTGG CTGCAGTCTT 
GGCGACCTTC GCGGACTCAT GGAGGCTCGC GGAGCTGAGG CGATAGTTCG GCTGTCCACA 
GAACACGAAG GCGTCGAGGG ATTGTGCAAG AAATTGAAGA CTGACTCGTT GGTTGGTCTC 
AATGGTGAAC AAGCAGATCT CGATCGACGA CGACATGTCT ACGGAGCCAA TACTATTCCA 
CCAGCCAAAA GTAAAGGATT CGTGCGGCTC GTTTTGGATG CATGCAAGGA CCCGACTCTT 
GTTATCCTGG TACTTTCCGG CTTCATCAAT TTAGCTCTAT CATTCTACGA ACCGACATCG 
GCGGCCGAAG ACGCAACTCA ACACCTTGTC AACGCGACGA CGGCGGCAAT TCTTGCCAAT 
GGAACCTTTA TGTCAACCAC CGAAGCACCA TCTGAAGGTC ATGGAACTGC ATGGATTGAA 
GGAGTTGCTA TTCTTCTCTG TGTTATTGTC GTCGTGCTGG TCACCGCTGT CAACGATTAT 
TCGAAAGAAC GACAGTTTAG AAGTTTGCAG GAAAAGATTG AAACTGGCCA AAAGTTCTCT 
GTTATTCGTA ATGGGGAGGC TATTGATGTC CCCGTCTCTG ATCTGGTAGT CGGTGACATT 
GCACGTGTCA AATATGGTGA TCTTCTTCCT GCCGATGGAT TTTTGATTCA GAGTAATGAT 
TTGAAGATCG ATGAATCATC CCTCACTGGA GAATCAGATC ACATCAAAAA GAGCATTGAA 
TCCGATCCAG TTCTCCTTTC GGGAACATAT GCTATGGAAG GTTCCGGAAA AATGCTCATC 
ACTGCCGTAG GAGTCAACTC ACAAACTGGT ATTATCATGA CTCTTCTCGG GGCTGGAAAA 
GCTGGAATCG GAGATGATGA CTCTACATCA ACATCTTCAA GTTCTTCTTC ATCATCTAGT 
TCATCCGGAA GTTCGTCTAA CGGAAGCTCT GACAGCTCAA AATCTGGCGA TGACGACTTG 
ACAGCCAAAT CAGTTCTGCA AGCTAAACTT TCCAAGTTGG CCCTTCAAAT CATCTATTGT 
GGAACTACAA TTGCCATCAT TGCACTTATC GTCTTGGTCA CTCGATTCTG TCTTGACCAC 
TATGTTTTTG AAAAGAACGA GTTCTCACTG GTCGATATTC AAATGTTCGT CAAGTTCTTC 
ATTATTGCTG TTACCATCTT GGTCATTTCC ATACCTGAAG GTCTTCCATT AGCTATTGCT 
CTTGCTCTTA CTTACTCTGT CAGAAAAATG ATGCACGATA ACAACTTGGT CCGTCACTTG 
GATGCATGTG AGACTATGGG AAATGCTACT TCAATTTGCT CTGACAAAAC TGGAACTCTC 
ACTACCAACC GAATGACTGT TGTTCAATCA TACATCAACG GAAACCATTA CACCAGTCAA 
GAAGCTCAAC CACATGGAGC CAACCTTCCA GGAAGCACTG GCCCAATTCT TATGGAGGCT 
ATCTCAGTCA ACTGTGCCTA TAACTCAATG ATTGTGGAGC CAACAAAAGC TGGAGAGCAA 
ATTCAGCAAC TTGGAAACAA GACCGAATGT GGTCTTCTAG GATTTGTAAA CCGTCTTGGA 
GGTGACTATG CTGCCATCAG AAAGAAGTTC CCAGAACATG ACCTCACCAA GGTCTACACT 
TTCAACTCTT CTCGTAAATG CATGATGACT GTTGTGCCAT ATGCCGAGAA TGGCCAGAAT 
ATCGGATACC GTGTCTATTG CAAAGGAGCC TCTGAGATTG TGCTCGGCAG ATGTACATAC 
TTGATCGGTT CTGACGGAAA GCCACATCAG CTTACTGGTG ATCGATTGAA GGAGATTACA 
TCTACTATCA TTCATGAGAT GGCTAACAGT GGTCTGAGAA CTATCTGTGT TGCTTATAAG 
ACAATCATCA AGAAGGGAAC AAGAGATGTG GAGAAGACTG AGATCGAATT CGCCGAAGAC 
TCTGATATCG ATTGGGATGA CGAGGATGCC ATGTACCAAA ACTTCACCGG AATTGCTATT 
TGCGGAATTC AAGATCCAGT TCGACCAGAG GTACCAGTCG CAATTTCAAA ATGCAAAAAA 
GCTGGAATCA CTGTAAGAAT GGTTACTGGT GATAACATCA TGACCGCCAG AGCAATTGCA 
ATGTCCTGTA AAATCTTGGA ACCCGGAGAG GACTTTCTGG CTCTTGAAGG AAAGGAATTC 
AATGAGAGAA TCCGCGACGA AAATGGAAAA GTCTCTCAGG CAAAACTTGA TGAAATCTGG 
CCACGTCTTC GTGTGTTGGC TCGTGCTCAA CCTGCTGATA AGTACACTCT TGTTAAGGGA 
ATTATCGACT CAAAAGCTAC TCCACAAAGA GAAATTGTTG CTGTAACTGG AGATGGTACA 
AATGATGGTC CAGCTTTGAA GAAGGCTGAT GTTGGATTCG CTATGGGAAT TGCTGGAACT 
GACGTTGCAA AGGAAGCTTC TGATATTATT TTGACTGATG ATAATTTCAC TTCAATTGTT 
AAAGCAGTTA TGTGGGGAAG AAATGTGTAC GATTCCATCT CCAAGTTCTT GCAATTCCAA 
CTCACTGTCA ATGTCGTCGC TGTCATCACT GCCTTCGTTG GAGCTGTCAC AGTGTCTGAT 
TCACCATTGA AGGCAGTTCA CATGCTTTGG ATTAATCTTA TCATGGACAC TCTTGCTTCA 
TTGGCCCTCG CAACTGAACA GCCAACTGAT GAACTACTCG AAAGAAAACC ATACGGTCGC 
AAGAAGTCGC TCATCTCAAG AACAATGGTC AAGAATATTC TTTGTCATGC TCTTTACCAA 
CTTATCATTA TCTTTGTCAT CTTCTTCTAC GGAGACACCA TCTTTGGAAT CAAGACAGGA 
CTCTATGCAC CACTCTTCGC TCCACCATCA CAACATTTCA CTTTGGTTTT CAATGCATTC 
GTTATGATGA CTGTCTTCAA TGAGATCAAT GCTCGTAAGG TTCACGGAGA GCGTAACGTG 
TTTAAAGGAT TGGCATCTAA CCGAGTTTTC TGTGTCATTT GGGTTACAAC ATTCATTGCT 
CAAATCATTA TTGTTCAATT CGGAGGTGCC TGGTTCTCAA CTGCTCCACT TACGCTTCAA 
CAATGGATTG TATGCCTCGT TCTTGGATTC TCAACTCTTA TCTGGGGACA AATTGTGGCA 
ACTATCCCAT CAAAGAAGCT ACCAAAGGCC TGGAAAGTTG GAAAGGGAGA AGTTCAACCA 
GCCAATCTTC ACATCAATGG AGACTACAAT GTCCGTGCTC GTTCCCGTGC GGTTACTCTT 
CGCAGATCTG GAAAGTCACT CTGGGTTAGA GGAATGTTCA TTATTGGAAA CCATCTCCGC 
GTTCTTCGTG CTTTCGGAAT GGAAAAGAGT GAAAAGGCTG CCTTCGGACG TACTGCTCCA 
GCAATGACGG CTGAAGCTGC TGAACGCTGG CGTGCCAGTT ACCGCAAGTA CCGTCACCAG 
AAACATCAAG AGAAGAAAGC CACCGCGGAA ACTGCTGAAT CTGTGAAATC TGCCGACTGG 
GCCAAGGAAC AGAAGGAAAA GAAGAAGACG TTCAAACAGA TCAAACAGGT TGCACGTGGA 
AAGAGTCTTG ACAAGGATTC TAAGAAGCAT CACAAGAAGA GGAAGGATCA GACAAATGTC 
GATATGGAAG ACATTGAACT CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023426.1
CDS	1..3687
Translation	MRLRRGHTYDEDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001028255.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028255.2

ORF Insert Sequence:

ATGAGATTGA GAAGAGGTCA TACCTACGAT GAGGATGCAA AAGAATTTGG CTGCAGTCTT 
GGCGACCTTC GCGGACTCAT GGAGGCTCGC GGAGCTGAGG CGATAGTTCG GCTGTCCACA 
GAACACGAAG GCGTCGAGGG ATTGTGCAAG AAATTGAAGA CTGACTCGTT GGTTGGTCTC 
AATGGTGAAC AAGCAGATCT CGATCGACGA CGACATGTCT ACGGAGCCAA TACTATTCCA 
CCAGCCAAAA GTAAAGGATT CGTGCGGCTC GTTTTGGATG CATGCAAGGA CCCGACTCTT 
GTTATCCTGG TACTTTCCGG CTTCATCAAT TTAGCTCTAT CATTCTACGA ACCGACATCG 
GCGGCCGAAG ACGCAACTCA ACACCTTGTC AACGCGACGA CGGCGGCAAT TCTTGCCAAT 
GGAACCTTTA TGTCAACCAC CGAAGCACCA TCTGAAGGTC ATGGAACTGC ATGGATTGAA 
GGAGTTGCTA TTCTTCTCTG TGTTATTGTC GTCGTGCTGG TCACCGCTGT CAACGATTAT 
TCGAAAGAAC GACAGTTTAG AAGTTTGCAG GAAAAGATTG AAACTGGCCA AAAGTTCTCT 
GTTATTCGTA ATGGGGAGGC TATTGATGTC CCCGTCTCTG ATCTGGTAGT CGGTGACATT 
GCACGTGTCA AATATGGTGA TCTTCTTCCT GCCGATGGAT TTTTGATTCA GAGTAATGAT 
TTGAAGATCG ATGAATCATC CCTCACTGGA GAATCAGATC ACATCAAAAA GAGCATTGAA 
TCCGATCCAG TTCTCCTTTC GGGAACATAT GCTATGGAAG GTTCCGGAAA AATGCTCATC 
ACTGCCGTAG GAGTCAACTC ACAAACTGGT ATTATCATGA CTCTTCTCGG GGCTGGAAAA 
GCTGGAATCG GAGATGATGA CTCTACATCA ACATCTTCAA GTTCTTCTTC ATCATCTAGT 
TCATCCGGAA GTTCGTCTAA CGGAAGCTCT GACAGCTCAA AATCTGGCGA TGACGACTTG 
ACAGCCAAAT CAGTTCTGCA AGCTAAACTT TCCAAGTTGG CCCTTCAAAT CATCTATTGT 
GGAACTACAA TTGCCATCAT TGCACTTATC GTCTTGGTCA CTCGATTCTG TCTTGACCAC 
TATGTTTTTG AAAAGAACGA GTTCTCACTG GTCGATATTC AAATGTTCGT CAAGTTCTTC 
ATTATTGCTG TTACCATCTT GGTCATTTCC ATACCTGAAG GTCTTCCATT AGCTATTGCT 
CTTGCTCTTA CTTACTCTGT CAGAAAAATG ATGCACGATA ACAACTTGGT CCGTCACTTG 
GATGCATGTG AGACTATGGG AAATGCTACT TCAATTTGCT CTGACAAAAC TGGAACTCTC 
ACTACCAACC GAATGACTGT TGTTCAATCA TACATCAACG GAAACCATTA CACCAGTCAA 
GAAGCTCAAC CACATGGAGC CAACCTTCCA GGAAGCACTG GCCCAATTCT TATGGAGGCT 
ATCTCAGTCA ACTGTGCCTA TAACTCAATG ATTGTGGAGC CAACAAAAGC TGGAGAGCAA 
ATTCAGCAAC TTGGAAACAA GACCGAATGT GGTCTTCTAG GATTTGTAAA CCGTCTTGGA 
GGTGACTATG CTGCCATCAG AAAGAAGTTC CCAGAACATG ACCTCACCAA GGTCTACACT 
TTCAACTCTT CTCGTAAATG CATGATGACT GTTGTGCCAT ATGCCGAGAA TGGCCAGAAT 
ATCGGATACC GTGTCTATTG CAAAGGAGCC TCTGAGATTG TGCTCGGCAG ATGTACATAC 
TTGATCGGTT CTGACGGAAA GCCACATCAG CTTACTGGTG ATCGATTGAA GGAGATTACA 
TCTACTATCA TTCATGAGAT GGCTAACAGT GGTCTGAGAA CTATCTGTGT TGCTTATAAG 
ACAATCATCA AGAAGGGAAC AAGAGATGTG GAGAAGACTG AGATCGAATT CGCCGAAGAC 
TCTGATATCG ATTGGGATGA CGAGGATGCC ATGTACCAAA ACTTCACCGG AATTGCTATT 
TGCGGAATTC AAGATCCAGT TCGACCAGAG GTACCAGTCG CAATTTCAAA ATGCAAAAAA 
GCTGGAATCA CTGTAAGAAT GGTTACTGGT GATAACATCA TGACCGCCAG AGCAATTGCA 
ATGTCCTGTA AAATCTTGGA ACCCGGAGAG GACTTTCTGG CTCTTGAAGG AAAGGAATTC 
AATGAGAGAA TCCGCGACGA AAATGGAAAA GTCTCTCAGG CAAAACTTGA TGAAATCTGG 
CCACGTCTTC GTGTGTTGGC TCGTGCTCAA CCTGCTGATA AGTACACTCT TGTTAAGGGA 
ATTATCGACT CAAAAGCTAC TCCACAAAGA GAAATTGTTG CTGTAACTGG AGATGGTACA 
AATGATGGTC CAGCTTTGAA GAAGGCTGAT GTTGGATTCG CTATGGGAAT TGCTGGAACT 
GACGTTGCAA AGGAAGCTTC TGATATTATT TTGACTGATG ATAATTTCAC TTCAATTGTT 
AAAGCAGTTA TGTGGGGAAG AAATGTGTAC GATTCCATCT CCAAGTTCTT GCAATTCCAA 
CTCACTGTCA ATGTCGTCGC TGTCATCACT GCCTTCGTTG GAGCTGTCAC AGTGTCTGAT 
TCACCATTGA AGGCAGTTCA CATGCTTTGG ATTAATCTTA TCATGGACAC TCTTGCTTCA 
TTGGCCCTCG CAACTGAACA GCCAACTGAT GAACTACTCG AAAGAAAACC ATACGGTCGC 
AAGAAGTCGC TCATCTCAAG AACAATGGTC AAGAATATTC TTTGTCATGC TCTTTACCAA 
CTTATCATTA TCTTTGTCAT CTTCTTCTAC GGAGACACCA TCTTTGGAAT CAAGACAGGA 
CTCTATGCAC CACTCTTCGC TCCACCATCA CAACATTTCA CTTTGGTTTT CAATGCATTC 
GTTATGATGA CTGTCTTCAA TGAGATCAAT GCTCGTAAGG TTCACGGAGA GCGTAACGTG 
TTTAAAGGAT TGGCATCTAA CCGAGTTTTC TGTGTCATTT GGGTTACAAC ATTCATTGCT 
CAAATCATTA TTGTTCAATT CGGAGGTGCC TGGTTCTCAA CTGCTCCACT TACGCTTCAA 
CAATGGATTG TATGCCTCGT TCTTGGATTC TCAACTCTTA TCTGGGGACA AATTGTGGCA 
ACTATCCCAT CAAAGAAGCT ACCAAAGGCC TGGAAAGTTG GAAAGGGAGA AGTTCAACCA 
GCCAATCTTC ACATCAATGG AGACTACAAT GTCCGTGCTC GTTCCCGTGC GGTTACTCTT 
CGCAGATCTG GAAAGTCACT CTGGGTTAGA GGAATGTTCA TTATTGGAAA CCATCTCCGC 
GTTCTTCGTG CTTTCGGAAT GGAAAAGAGT GAAAAGGCTG CCTTCGGACG TACTGCTCCA 
GCAATGACGG CTGAAGCTGC TGAACGCTGG CGTGCCAGTT ACCGCAAGTA CCGTCACCAG 
AAACATCAAG AGAAGAAAGC CACCGCGGAA ACTGCTGAAT CTGTGAAATC TGCCGACTGG 
GCCAAGGAAC AGAAGGAAAA GAAGAAGACG TTCAAACAGA TCAAACAGGT TGCACGTGGA 
AAGAGTCTTG ACAAGGATTC TAAGAAGCAT CACAAGAAGA GGAAGGATCA GACAAATGTC 
GATATGGAAG ACATTGAACT CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001028255.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023426.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

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CloneID	OCa343223
Clone ID Related Accession (Same CDS sequence)	NM_001028255.2 , NM_001028255.3
Accession Version	NM_001028255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001028255.2.

ATGAGATTGA GAAGAGGTCA TACCTACGAT GAGGATGCAA AAGAATTTGG CTGCAGTCTT 
GGCGACCTTC GCGGACTCAT GGAGGCTCGC GGAGCTGAGG CGATAGTTCG GCTGTCCACA 
GAACACGAAG GCGTCGAGGG ATTGTGCAAG AAATTGAAGA CTGACTCGTT GGTTGGTCTC 
AATGGTGAAC AAGCAGATCT CGATCGACGA CGACATGTCT ACGGAGCCAA TACTATTCCA 
CCAGCCAAAA GTAAAGGATT CGTGCGGCTC GTTTTGGATG CATGCAAGGA CCCGACTCTT 
GTTATCCTGG TACTTTCCGG CTTCATCAAT TTAGCTCTAT CATTCTACGA ACCGACATCG 
GCGGCCGAAG ACGCAACTCA ACACCTTGTC AACGCGACGA CGGCGGCAAT TCTTGCCAAT 
GGAACCTTTA TGTCAACCAC CGAAGCACCA TCTGAAGGTC ATGGAACTGC ATGGATTGAA 
GGAGTTGCTA TTCTTCTCTG TGTTATTGTC GTCGTGCTGG TCACCGCTGT CAACGATTAT 
TCGAAAGAAC GACAGTTTAG AAGTTTGCAG GAAAAGATTG AAACTGGCCA AAAGTTCTCT 
GTTATTCGTA ATGGGGAGGC TATTGATGTC CCCGTCTCTG ATCTGGTAGT CGGTGACATT 
GCACGTGTCA AATATGGTGA TCTTCTTCCT GCCGATGGAT TTTTGATTCA GAGTAATGAT 
TTGAAGATCG ATGAATCATC CCTCACTGGA GAATCAGATC ACATCAAAAA GAGCATTGAA 
TCCGATCCAG TTCTCCTTTC GGGAACATAT GCTATGGAAG GTTCCGGAAA AATGCTCATC 
ACTGCCGTAG GAGTCAACTC ACAAACTGGT ATTATCATGA CTCTTCTCGG GGCTGGAAAA 
GCTGGAATCG GAGATGATGA CTCTACATCA ACATCTTCAA GTTCTTCTTC ATCATCTAGT 
TCATCCGGAA GTTCGTCTAA CGGAAGCTCT GACAGCTCAA AATCTGGCGA TGACGACTTG 
ACAGCCAAAT CAGTTCTGCA AGCTAAACTT TCCAAGTTGG CCCTTCAAAT CATCTATTGT 
GGAACTACAA TTGCCATCAT TGCACTTATC GTCTTGGTCA CTCGATTCTG TCTTGACCAC 
TATGTTTTTG AAAAGAACGA GTTCTCACTG GTCGATATTC AAATGTTCGT CAAGTTCTTC 
ATTATTGCTG TTACCATCTT GGTCATTTCC ATACCTGAAG GTCTTCCATT AGCTATTGCT 
CTTGCTCTTA CTTACTCTGT CAGAAAAATG ATGCACGATA ACAACTTGGT CCGTCACTTG 
GATGCATGTG AGACTATGGG AAATGCTACT TCAATTTGCT CTGACAAAAC TGGAACTCTC 
ACTACCAACC GAATGACTGT TGTTCAATCA TACATCAACG GAAACCATTA CACCAGTCAA 
GAAGCTCAAC CACATGGAGC CAACCTTCCA GGAAGCACTG GCCCAATTCT TATGGAGGCT 
ATCTCAGTCA ACTGTGCCTA TAACTCAATG ATTGTGGAGC CAACAAAAGC TGGAGAGCAA 
ATTCAGCAAC TTGGAAACAA GACCGAATGT GGTCTTCTAG GATTTGTAAA CCGTCTTGGA 
GGTGACTATG CTGCCATCAG AAAGAAGTTC CCAGAACATG ACCTCACCAA GGTCTACACT 
TTCAACTCTT CTCGTAAATG CATGATGACT GTTGTGCCAT ATGCCGAGAA TGGCCAGAAT 
ATCGGATACC GTGTCTATTG CAAAGGAGCC TCTGAGATTG TGCTCGGCAG ATGTACATAC 
TTGATCGGTT CTGACGGAAA GCCACATCAG CTTACTGGTG ATCGATTGAA GGAGATTACA 
TCTACTATCA TTCATGAGAT GGCTAACAGT GGTCTGAGAA CTATCTGTGT TGCTTATAAG 
ACAATCATCA AGAAGGGAAC AAGAGATGTG GAGAAGACTG AGATCGAATT CGCCGAAGAC 
TCTGATATCG ATTGGGATGA CGAGGATGCC ATGTACCAAA ACTTCACCGG AATTGCTATT 
TGCGGAATTC AAGATCCAGT TCGACCAGAG GTACCAGTCG CAATTTCAAA ATGCAAAAAA 
GCTGGAATCA CTGTAAGAAT GGTTACTGGT GATAACATCA TGACCGCCAG AGCAATTGCA 
ATGTCCTGTA AAATCTTGGA ACCCGGAGAG GACTTTCTGG CTCTTGAAGG AAAGGAATTC 
AATGAGAGAA TCCGCGACGA AAATGGAAAA GTCTCTCAGG CAAAACTTGA TGAAATCTGG 
CCACGTCTTC GTGTGTTGGC TCGTGCTCAA CCTGCTGATA AGTACACTCT TGTTAAGGGA 
ATTATCGACT CAAAAGCTAC TCCACAAAGA GAAATTGTTG CTGTAACTGG AGATGGTACA 
AATGATGGTC CAGCTTTGAA GAAGGCTGAT GTTGGATTCG CTATGGGAAT TGCTGGAACT 
GACGTTGCAA AGGAAGCTTC TGATATTATT TTGACTGATG ATAATTTCAC TTCAATTGTT 
AAAGCAGTTA TGTGGGGAAG AAATGTGTAC GATTCCATCT CCAAGTTCTT GCAATTCCAA 
CTCACTGTCA ATGTCGTCGC TGTCATCACT GCCTTCGTTG GAGCTGTCAC AGTGTCTGAT 
TCACCATTGA AGGCAGTTCA CATGCTTTGG ATTAATCTTA TCATGGACAC TCTTGCTTCA 
TTGGCCCTCG CAACTGAACA GCCAACTGAT GAACTACTCG AAAGAAAACC ATACGGTCGC 
AAGAAGTCGC TCATCTCAAG AACAATGGTC AAGAATATTC TTTGTCATGC TCTTTACCAA 
CTTATCATTA TCTTTGTCAT CTTCTTCTAC GGAGACACCA TCTTTGGAAT CAAGACAGGA 
CTCTATGCAC CACTCTTCGC TCCACCATCA CAACATTTCA CTTTGGTTTT CAATGCATTC 
GTTATGATGA CTGTCTTCAA TGAGATCAAT GCTCGTAAGG TTCACGGAGA GCGTAACGTG 
TTTAAAGGAT TGGCATCTAA CCGAGTTTTC TGTGTCATTT GGGTTACAAC ATTCATTGCT 
CAAATCATTA TTGTTCAATT CGGAGGTGCC TGGTTCTCAA CTGCTCCACT TACGCTTCAA 
CAATGGATTG TATGCCTCGT TCTTGGATTC TCAACTCTTA TCTGGGGACA AATTGTGGCA 
ACTATCCCAT CAAAGAAGCT ACCAAAGGCC TGGAAAGTTG GAAAGGGAGA AGTTCAACCA 
GCCAATCTTC ACATCAATGG AGACTACAAT GTCCGTGCTC GTTCCCGTGC GGTTACTCTT 
CGCAGATCTG GAAAGTCACT CTGGGTTAGA GGAATGTTCA TTATTGGAAA CCATCTCCGC 
GTTCTTCGTG CTTTCGGAAT GGAAAAGAGT GAAAAGGCTG CCTTCGGACG TACTGCTCCA 
GCAATGACGG CTGAAGCTGC TGAACGCTGG CGTGCCAGTT ACCGCAAGTA CCGTCACCAG 
AAACATCAAG AGAAGAAAGC CACCGCGGAA ACTGCTGAAT CTGTGAAATC TGCCGACTGG 
GCCAAGGAAC AGAAGGAAAA GAAGAAGACG TTCAAACAGA TCAAACAGGT TGCACGTGGA 
AAGAGTCTTG ACAAGGATTC TAAGAAGCAT CACAAGAAGA GGAAGGATCA GACAAATGTC 
GATATGGAAG ACATTGAACT CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023426.1
CDS	62..3748
Translation	MRLRRGHTYDEDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001028255.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028255.3

ORF Insert Sequence:

ATGAGATTGA GAAGAGGTCA TACCTACGAT GAGGATGCAA AAGAATTTGG CTGCAGTCTT 
GGCGACCTTC GCGGACTCAT GGAGGCTCGC GGAGCTGAGG CGATAGTTCG GCTGTCCACA 
GAACACGAAG GCGTCGAGGG ATTGTGCAAG AAATTGAAGA CTGACTCGTT GGTTGGTCTC 
AATGGTGAAC AAGCAGATCT CGATCGACGA CGACATGTCT ACGGAGCCAA TACTATTCCA 
CCAGCCAAAA GTAAAGGATT CGTGCGGCTC GTTTTGGATG CATGCAAGGA CCCGACTCTT 
GTTATCCTGG TACTTTCCGG CTTCATCAAT TTAGCTCTAT CATTCTACGA ACCGACATCG 
GCGGCCGAAG ACGCAACTCA ACACCTTGTC AACGCGACGA CGGCGGCAAT TCTTGCCAAT 
GGAACCTTTA TGTCAACCAC CGAAGCACCA TCTGAAGGTC ATGGAACTGC ATGGATTGAA 
GGAGTTGCTA TTCTTCTCTG TGTTATTGTC GTCGTGCTGG TCACCGCTGT CAACGATTAT 
TCGAAAGAAC GACAGTTTAG AAGTTTGCAG GAAAAGATTG AAACTGGCCA AAAGTTCTCT 
GTTATTCGTA ATGGGGAGGC TATTGATGTC CCCGTCTCTG ATCTGGTAGT CGGTGACATT 
GCACGTGTCA AATATGGTGA TCTTCTTCCT GCCGATGGAT TTTTGATTCA GAGTAATGAT 
TTGAAGATCG ATGAATCATC CCTCACTGGA GAATCAGATC ACATCAAAAA GAGCATTGAA 
TCCGATCCAG TTCTCCTTTC GGGAACATAT GCTATGGAAG GTTCCGGAAA AATGCTCATC 
ACTGCCGTAG GAGTCAACTC ACAAACTGGT ATTATCATGA CTCTTCTCGG GGCTGGAAAA 
GCTGGAATCG GAGATGATGA CTCTACATCA ACATCTTCAA GTTCTTCTTC ATCATCTAGT 
TCATCCGGAA GTTCGTCTAA CGGAAGCTCT GACAGCTCAA AATCTGGCGA TGACGACTTG 
ACAGCCAAAT CAGTTCTGCA AGCTAAACTT TCCAAGTTGG CCCTTCAAAT CATCTATTGT 
GGAACTACAA TTGCCATCAT TGCACTTATC GTCTTGGTCA CTCGATTCTG TCTTGACCAC 
TATGTTTTTG AAAAGAACGA GTTCTCACTG GTCGATATTC AAATGTTCGT CAAGTTCTTC 
ATTATTGCTG TTACCATCTT GGTCATTTCC ATACCTGAAG GTCTTCCATT AGCTATTGCT 
CTTGCTCTTA CTTACTCTGT CAGAAAAATG ATGCACGATA ACAACTTGGT CCGTCACTTG 
GATGCATGTG AGACTATGGG AAATGCTACT TCAATTTGCT CTGACAAAAC TGGAACTCTC 
ACTACCAACC GAATGACTGT TGTTCAATCA TACATCAACG GAAACCATTA CACCAGTCAA 
GAAGCTCAAC CACATGGAGC CAACCTTCCA GGAAGCACTG GCCCAATTCT TATGGAGGCT 
ATCTCAGTCA ACTGTGCCTA TAACTCAATG ATTGTGGAGC CAACAAAAGC TGGAGAGCAA 
ATTCAGCAAC TTGGAAACAA GACCGAATGT GGTCTTCTAG GATTTGTAAA CCGTCTTGGA 
GGTGACTATG CTGCCATCAG AAAGAAGTTC CCAGAACATG ACCTCACCAA GGTCTACACT 
TTCAACTCTT CTCGTAAATG CATGATGACT GTTGTGCCAT ATGCCGAGAA TGGCCAGAAT 
ATCGGATACC GTGTCTATTG CAAAGGAGCC TCTGAGATTG TGCTCGGCAG ATGTACATAC 
TTGATCGGTT CTGACGGAAA GCCACATCAG CTTACTGGTG ATCGATTGAA GGAGATTACA 
TCTACTATCA TTCATGAGAT GGCTAACAGT GGTCTGAGAA CTATCTGTGT TGCTTATAAG 
ACAATCATCA AGAAGGGAAC AAGAGATGTG GAGAAGACTG AGATCGAATT CGCCGAAGAC 
TCTGATATCG ATTGGGATGA CGAGGATGCC ATGTACCAAA ACTTCACCGG AATTGCTATT 
TGCGGAATTC AAGATCCAGT TCGACCAGAG GTACCAGTCG CAATTTCAAA ATGCAAAAAA 
GCTGGAATCA CTGTAAGAAT GGTTACTGGT GATAACATCA TGACCGCCAG AGCAATTGCA 
ATGTCCTGTA AAATCTTGGA ACCCGGAGAG GACTTTCTGG CTCTTGAAGG AAAGGAATTC 
AATGAGAGAA TCCGCGACGA AAATGGAAAA GTCTCTCAGG CAAAACTTGA TGAAATCTGG 
CCACGTCTTC GTGTGTTGGC TCGTGCTCAA CCTGCTGATA AGTACACTCT TGTTAAGGGA 
ATTATCGACT CAAAAGCTAC TCCACAAAGA GAAATTGTTG CTGTAACTGG AGATGGTACA 
AATGATGGTC CAGCTTTGAA GAAGGCTGAT GTTGGATTCG CTATGGGAAT TGCTGGAACT 
GACGTTGCAA AGGAAGCTTC TGATATTATT TTGACTGATG ATAATTTCAC TTCAATTGTT 
AAAGCAGTTA TGTGGGGAAG AAATGTGTAC GATTCCATCT CCAAGTTCTT GCAATTCCAA 
CTCACTGTCA ATGTCGTCGC TGTCATCACT GCCTTCGTTG GAGCTGTCAC AGTGTCTGAT 
TCACCATTGA AGGCAGTTCA CATGCTTTGG ATTAATCTTA TCATGGACAC TCTTGCTTCA 
TTGGCCCTCG CAACTGAACA GCCAACTGAT GAACTACTCG AAAGAAAACC ATACGGTCGC 
AAGAAGTCGC TCATCTCAAG AACAATGGTC AAGAATATTC TTTGTCATGC TCTTTACCAA 
CTTATCATTA TCTTTGTCAT CTTCTTCTAC GGAGACACCA TCTTTGGAAT CAAGACAGGA 
CTCTATGCAC CACTCTTCGC TCCACCATCA CAACATTTCA CTTTGGTTTT CAATGCATTC 
GTTATGATGA CTGTCTTCAA TGAGATCAAT GCTCGTAAGG TTCACGGAGA GCGTAACGTG 
TTTAAAGGAT TGGCATCTAA CCGAGTTTTC TGTGTCATTT GGGTTACAAC ATTCATTGCT 
CAAATCATTA TTGTTCAATT CGGAGGTGCC TGGTTCTCAA CTGCTCCACT TACGCTTCAA 
CAATGGATTG TATGCCTCGT TCTTGGATTC TCAACTCTTA TCTGGGGACA AATTGTGGCA 
ACTATCCCAT CAAAGAAGCT ACCAAAGGCC TGGAAAGTTG GAAAGGGAGA AGTTCAACCA 
GCCAATCTTC ACATCAATGG AGACTACAAT GTCCGTGCTC GTTCCCGTGC GGTTACTCTT 
CGCAGATCTG GAAAGTCACT CTGGGTTAGA GGAATGTTCA TTATTGGAAA CCATCTCCGC 
GTTCTTCGTG CTTTCGGAAT GGAAAAGAGT GAAAAGGCTG CCTTCGGACG TACTGCTCCA 
GCAATGACGG CTGAAGCTGC TGAACGCTGG CGTGCCAGTT ACCGCAAGTA CCGTCACCAG 
AAACATCAAG AGAAGAAAGC CACCGCGGAA ACTGCTGAAT CTGTGAAATC TGCCGACTGG 
GCCAAGGAAC AGAAGGAAAA GAAGAAGACG TTCAAACAGA TCAAACAGGT TGCACGTGGA 
AAGAGTCTTG ACAAGGATTC TAAGAAGCAT CACAAGAAGA GGAAGGATCA GACAAATGTC 
GATATGGAAG ACATTGAACT CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001268480.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255409.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

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CloneID	OCa343609
Clone ID Related Accession (Same CDS sequence)	NM_001268480.1 , NM_001268480.2
Accession Version	NM_001268480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTCAACCA CCGAAGCACC ATCTGAAGGT CATGGAACTG CATGGATTGA AGGAGTTGCT 
ATTCTTCTCT GTGTTATTGT CGTCGTGCTG GTCACCGCTG TCAACGATTA TTCGAAAGAA 
CGACAGTTTA GAAGTTTGCA GGAAAAGATT GAAACTGGCC AAAAGTTCTC TGTTATTCGT 
AATGGGGAGG CTATTGATGT CCCCGTCTCT GATCTGGTAG TCGGTGACAT TGCACGTGTC 
AAATATGGTG ATCTTCTTCC TGCCGATGGA TTTTTGATTC AGAGTAATGA TTTGAAGATC 
GATGAATCAT CCCTCACTGG AGAATCAGAT CACATCAAAA AGAGCATTGA ATCCGATCCA 
GTTCTCCTTT CGGGAACATA TGCTATGGAA GGTTCCGGAA AAATGCTCAT CACTGCCGTA 
GGAGTCAACT CACAAACTGG TATTATCATG ACTCTTCTCG GGGCTGGAAA AGCTGGAATC 
GGAGATGATG ACTCTACATC AACATCTTCA AGTTCTTCTT CATCATCTAG TTCATCCGGA 
AGTTCGTCTA ACGGAAGCTC TGACAGCTCA AAATCTGGCG ATGACGACTT GACAGCCAAA 
TCAGTTCTGC AAGCTAAACT TTCCAAGTTG GCCCTTCAAA TCATCTATTG TGGAACTACA 
ATTGCCATCA TTGCACTTAT CGTCTTGGTC ACTCGATTCT GTCTTGACCA CTATGTTTTT 
GAAAAGAACG AGTTCTCACT GGTCGATATT CAAATGTTCG TCAAGTTCTT CATTATTGCT 
GTTACCATCT TGGTCATTTC CATACCTGAA GGTCTTCCAT TAGCTATTGC TCTTGCTCTT 
ACTTACTCTG TCAGAAAAAT GATGCACGAT AACAACTTGG TCCGTCACTT GGATGCATGT 
GAGACTATGG GAAATGCTAC TTCAATTTGC TCTGACAAAA CTGGAACTCT CACTACCAAC 
CGAATGACTG TTGTTCAATC ATACATCAAC GGAAACCATT ACACCAGTCA AGAAGCTCAA 
CCACATGGAG CCAACCTTCC AGGAAGCACT GGCCCAATTC TTATGGAGGC TATCTCAGTC 
AACTGTGCCT ATAACTCAAT GATTGTGGAG CCAACAAAAG CTGGAGAGCA AATTCAGCAA 
CTTGGAAACA AGACCGAATG TGGTCTTCTA GGATTTGTAA ACCGTCTTGG AGGTGACTAT 
GCTGCCATCA GAAAGAAGTT CCCAGAACAT GACCTCACCA AGGTCTACAC TTTCAACTCT 
TCTCGTAAAT GCATGATGAC TGTTGTGCCA TATGCCGAGA ATGGCCAGAA TATCGGATAC 
CGTGTCTATT GCAAAGGAGC CTCTGAGATT GTGCTCGGCA GATGTACATA CTTGATCGGT 
TCTGACGGAA AGCCACATCA GCTTACTGGT GATCGATTGA AGGAGATTAC ATCTACTATC 
ATTCATGAGA TGGCTAACAG TGGTCTGAGA ACTATCTGTG TTGCTTATAA GACAATCATC 
AAGAAGGGAA CAAGAGATGT GGAGAAGACT GAGATCGAAT TCGCCGAAGA CTCTGATATC 
GATTGGGATG ACGAGGATGC CATGTACCAA AACTTCACCG GAATTGCTAT TTGCGGAATT 
CAAGATCCAG TTCGACCAGA GGTACCAGTC GCAATTTCAA AATGCAAAAA AGCTGGAATC 
ACTGTAAGAA TGGTTACTGG TGATAACATC ATGACCGCCA GAGCAATTGC AATGTCCTGT 
AAAATCTTGG AACCCGGAGA GGACTTTCTG GCTCTTGAAG GAAAGGAATT CAATGAGAGA 
ATCCGCGACG AAAATGGAAA AGTCTCTCAG GCAAAACTTG ATGAAATCTG GCCACGTCTT 
CGTGTGTTGG CTCGTGCTCA ACCTGCTGAT AAGTACACTC TTGTTAAGGG AATTATCGAC 
TCAAAAGCTA CTCCACAAAG AGAAATTGTT GCTGTAACTG GAGATGGTAC AAATGATGGT 
CCAGCTTTGA AGAAGGCTGA TGTTGGATTC GCTATGGGAA TTGCTGGAAC TGACGTTGCA 
AAGGAAGCTT CTGATATTAT TTTGACTGAT GATAATTTCA CTTCAATTGT TAAAGCAGTT 
ATGTGGGGAA GAAATGTGTA CGATTCCATC TCCAAGTTCT TGCAATTCCA ACTCACTGTC 
AATGTCGTCG CTGTCATCAC TGCCTTCGTT GGAGCTGTCA CAGTGTCTGA TTCACCATTG 
AAGGCAGTTC ACATGCTTTG GATTAATCTT ATCATGGACA CTCTTGCTTC ATTGGCCCTC 
GCAACTGAAC AGCCAACTGA TGAACTACTC GAAAGAAAAC CATACGGTCG CAAGAAGTCG 
CTCATCTCAA GAACAATGGT CAAGAATATT CTTTGTCATG CTCTTTACCA ACTTATCATT 
ATCTTTGTCA TCTTCTTCTA CGGAGACACC ATCTTTGGAA TCAAGACAGG ACTCTATGCA 
CCACTCTTCG CTCCACCATC ACAACATTTC ACTTTGGTTT TCAATGCATT CGTTATGATG 
ACTGTCTTCA ATGAGATCAA TGCTCGTAAG GTTCACGGAG AGCGTAACGT GTTTAAAGGA 
TTGGCATCTA ACCGAGTTTT CTGTGTCATT TGGGTTACAA CATTCATTGC TCAAATCATT 
ATTGTTCAAT TCGGAGGTGC CTGGTTCTCA ACTGCTCCAC TTACGCTTCA ACAATGGATT 
GTATGCCTCG TTCTTGGATT CTCAACTCTT ATCTGGGGAC AAATTGTGGC AACTATCCCA 
TCAAAGAAGC TACCAAAGGC CTGGAAAGTT GGAAAGGGAG AAGTTCAACC AGCCAATCTT 
CACATCAATG GAGACTACAA TGTCCGTGCT CGTTCCCGTG CGGTTACTCT TCGCAGATCT 
GGAAAGTCAC TCTGGGTTAG AGGAATGTTC ATTATTGGAA ACCATCTCCG CGTTCTTCGT 
GCTTTCGGAA TGGAAAAGAG TGAAAAGGCT GCCTTCGGAC GTACTGCTCC AGCAATGACG 
GCTGAAGCTG CTGAACGCTG GCGTGCCAGT TACCGCAAGT ACCGTCACCA GAAACATCAA 
GAGAAGAAAG CCACCGCGGA AACTGCTGAA TCTGTGAAAT CTGCCGACTG GGCCAAGGAA 
CAGAAGGAAA AGAAGAAGAC GTTCAAACAG ATCAAACAGG TTGCACGTGG AAAGAGTCTT 
GACAAGGATT CTAAGAAGCA TCACAAGAAG AGGAAGGATC AGACAAATGT CGATATGGAA 
GACATTGAAC TCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255409.1
CDS	1..3258
Translation	MSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001268480.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268480.1

ORF Insert Sequence:

ATGTCAACCA CCGAAGCACC ATCTGAAGGT CATGGAACTG CATGGATTGA AGGAGTTGCT 
ATTCTTCTCT GTGTTATTGT CGTCGTGCTG GTCACCGCTG TCAACGATTA TTCGAAAGAA 
CGACAGTTTA GAAGTTTGCA GGAAAAGATT GAAACTGGCC AAAAGTTCTC TGTTATTCGT 
AATGGGGAGG CTATTGATGT CCCCGTCTCT GATCTGGTAG TCGGTGACAT TGCACGTGTC 
AAATATGGTG ATCTTCTTCC TGCCGATGGA TTTTTGATTC AGAGTAATGA TTTGAAGATC 
GATGAATCAT CCCTCACTGG AGAATCAGAT CACATCAAAA AGAGCATTGA ATCCGATCCA 
GTTCTCCTTT CGGGAACATA TGCTATGGAA GGTTCCGGAA AAATGCTCAT CACTGCCGTA 
GGAGTCAACT CACAAACTGG TATTATCATG ACTCTTCTCG GGGCTGGAAA AGCTGGAATC 
GGAGATGATG ACTCTACATC AACATCTTCA AGTTCTTCTT CATCATCTAG TTCATCCGGA 
AGTTCGTCTA ACGGAAGCTC TGACAGCTCA AAATCTGGCG ATGACGACTT GACAGCCAAA 
TCAGTTCTGC AAGCTAAACT TTCCAAGTTG GCCCTTCAAA TCATCTATTG TGGAACTACA 
ATTGCCATCA TTGCACTTAT CGTCTTGGTC ACTCGATTCT GTCTTGACCA CTATGTTTTT 
GAAAAGAACG AGTTCTCACT GGTCGATATT CAAATGTTCG TCAAGTTCTT CATTATTGCT 
GTTACCATCT TGGTCATTTC CATACCTGAA GGTCTTCCAT TAGCTATTGC TCTTGCTCTT 
ACTTACTCTG TCAGAAAAAT GATGCACGAT AACAACTTGG TCCGTCACTT GGATGCATGT 
GAGACTATGG GAAATGCTAC TTCAATTTGC TCTGACAAAA CTGGAACTCT CACTACCAAC 
CGAATGACTG TTGTTCAATC ATACATCAAC GGAAACCATT ACACCAGTCA AGAAGCTCAA 
CCACATGGAG CCAACCTTCC AGGAAGCACT GGCCCAATTC TTATGGAGGC TATCTCAGTC 
AACTGTGCCT ATAACTCAAT GATTGTGGAG CCAACAAAAG CTGGAGAGCA AATTCAGCAA 
CTTGGAAACA AGACCGAATG TGGTCTTCTA GGATTTGTAA ACCGTCTTGG AGGTGACTAT 
GCTGCCATCA GAAAGAAGTT CCCAGAACAT GACCTCACCA AGGTCTACAC TTTCAACTCT 
TCTCGTAAAT GCATGATGAC TGTTGTGCCA TATGCCGAGA ATGGCCAGAA TATCGGATAC 
CGTGTCTATT GCAAAGGAGC CTCTGAGATT GTGCTCGGCA GATGTACATA CTTGATCGGT 
TCTGACGGAA AGCCACATCA GCTTACTGGT GATCGATTGA AGGAGATTAC ATCTACTATC 
ATTCATGAGA TGGCTAACAG TGGTCTGAGA ACTATCTGTG TTGCTTATAA GACAATCATC 
AAGAAGGGAA CAAGAGATGT GGAGAAGACT GAGATCGAAT TCGCCGAAGA CTCTGATATC 
GATTGGGATG ACGAGGATGC CATGTACCAA AACTTCACCG GAATTGCTAT TTGCGGAATT 
CAAGATCCAG TTCGACCAGA GGTACCAGTC GCAATTTCAA AATGCAAAAA AGCTGGAATC 
ACTGTAAGAA TGGTTACTGG TGATAACATC ATGACCGCCA GAGCAATTGC AATGTCCTGT 
AAAATCTTGG AACCCGGAGA GGACTTTCTG GCTCTTGAAG GAAAGGAATT CAATGAGAGA 
ATCCGCGACG AAAATGGAAA AGTCTCTCAG GCAAAACTTG ATGAAATCTG GCCACGTCTT 
CGTGTGTTGG CTCGTGCTCA ACCTGCTGAT AAGTACACTC TTGTTAAGGG AATTATCGAC 
TCAAAAGCTA CTCCACAAAG AGAAATTGTT GCTGTAACTG GAGATGGTAC AAATGATGGT 
CCAGCTTTGA AGAAGGCTGA TGTTGGATTC GCTATGGGAA TTGCTGGAAC TGACGTTGCA 
AAGGAAGCTT CTGATATTAT TTTGACTGAT GATAATTTCA CTTCAATTGT TAAAGCAGTT 
ATGTGGGGAA GAAATGTGTA CGATTCCATC TCCAAGTTCT TGCAATTCCA ACTCACTGTC 
AATGTCGTCG CTGTCATCAC TGCCTTCGTT GGAGCTGTCA CAGTGTCTGA TTCACCATTG 
AAGGCAGTTC ACATGCTTTG GATTAATCTT ATCATGGACA CTCTTGCTTC ATTGGCCCTC 
GCAACTGAAC AGCCAACTGA TGAACTACTC GAAAGAAAAC CATACGGTCG CAAGAAGTCG 
CTCATCTCAA GAACAATGGT CAAGAATATT CTTTGTCATG CTCTTTACCA ACTTATCATT 
ATCTTTGTCA TCTTCTTCTA CGGAGACACC ATCTTTGGAA TCAAGACAGG ACTCTATGCA 
CCACTCTTCG CTCCACCATC ACAACATTTC ACTTTGGTTT TCAATGCATT CGTTATGATG 
ACTGTCTTCA ATGAGATCAA TGCTCGTAAG GTTCACGGAG AGCGTAACGT GTTTAAAGGA 
TTGGCATCTA ACCGAGTTTT CTGTGTCATT TGGGTTACAA CATTCATTGC TCAAATCATT 
ATTGTTCAAT TCGGAGGTGC CTGGTTCTCA ACTGCTCCAC TTACGCTTCA ACAATGGATT 
GTATGCCTCG TTCTTGGATT CTCAACTCTT ATCTGGGGAC AAATTGTGGC AACTATCCCA 
TCAAAGAAGC TACCAAAGGC CTGGAAAGTT GGAAAGGGAG AAGTTCAACC AGCCAATCTT 
CACATCAATG GAGACTACAA TGTCCGTGCT CGTTCCCGTG CGGTTACTCT TCGCAGATCT 
GGAAAGTCAC TCTGGGTTAG AGGAATGTTC ATTATTGGAA ACCATCTCCG CGTTCTTCGT 
GCTTTCGGAA TGGAAAAGAG TGAAAAGGCT GCCTTCGGAC GTACTGCTCC AGCAATGACG 
GCTGAAGCTG CTGAACGCTG GCGTGCCAGT TACCGCAAGT ACCGTCACCA GAAACATCAA 
GAGAAGAAAG CCACCGCGGA AACTGCTGAA TCTGTGAAAT CTGCCGACTG GGCCAAGGAA 
CAGAAGGAAA AGAAGAAGAC GTTCAAACAG ATCAAACAGG TTGCACGTGG AAAGAGTCTT 
GACAAGGATT CTAAGAAGCA TCACAAGAAG AGGAAGGATC AGACAAATGT CGATATGGAA 
GACATTGAAC TCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001268480.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255409.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

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CloneID	OCa343609
Clone ID Related Accession (Same CDS sequence)	NM_001268480.1 , NM_001268480.2
Accession Version	NM_001268480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268480.1. COMPLETENESS: incomplete on the 5' end.

ATGTCAACCA CCGAAGCACC ATCTGAAGGT CATGGAACTG CATGGATTGA AGGAGTTGCT 
ATTCTTCTCT GTGTTATTGT CGTCGTGCTG GTCACCGCTG TCAACGATTA TTCGAAAGAA 
CGACAGTTTA GAAGTTTGCA GGAAAAGATT GAAACTGGCC AAAAGTTCTC TGTTATTCGT 
AATGGGGAGG CTATTGATGT CCCCGTCTCT GATCTGGTAG TCGGTGACAT TGCACGTGTC 
AAATATGGTG ATCTTCTTCC TGCCGATGGA TTTTTGATTC AGAGTAATGA TTTGAAGATC 
GATGAATCAT CCCTCACTGG AGAATCAGAT CACATCAAAA AGAGCATTGA ATCCGATCCA 
GTTCTCCTTT CGGGAACATA TGCTATGGAA GGTTCCGGAA AAATGCTCAT CACTGCCGTA 
GGAGTCAACT CACAAACTGG TATTATCATG ACTCTTCTCG GGGCTGGAAA AGCTGGAATC 
GGAGATGATG ACTCTACATC AACATCTTCA AGTTCTTCTT CATCATCTAG TTCATCCGGA 
AGTTCGTCTA ACGGAAGCTC TGACAGCTCA AAATCTGGCG ATGACGACTT GACAGCCAAA 
TCAGTTCTGC AAGCTAAACT TTCCAAGTTG GCCCTTCAAA TCATCTATTG TGGAACTACA 
ATTGCCATCA TTGCACTTAT CGTCTTGGTC ACTCGATTCT GTCTTGACCA CTATGTTTTT 
GAAAAGAACG AGTTCTCACT GGTCGATATT CAAATGTTCG TCAAGTTCTT CATTATTGCT 
GTTACCATCT TGGTCATTTC CATACCTGAA GGTCTTCCAT TAGCTATTGC TCTTGCTCTT 
ACTTACTCTG TCAGAAAAAT GATGCACGAT AACAACTTGG TCCGTCACTT GGATGCATGT 
GAGACTATGG GAAATGCTAC TTCAATTTGC TCTGACAAAA CTGGAACTCT CACTACCAAC 
CGAATGACTG TTGTTCAATC ATACATCAAC GGAAACCATT ACACCAGTCA AGAAGCTCAA 
CCACATGGAG CCAACCTTCC AGGAAGCACT GGCCCAATTC TTATGGAGGC TATCTCAGTC 
AACTGTGCCT ATAACTCAAT GATTGTGGAG CCAACAAAAG CTGGAGAGCA AATTCAGCAA 
CTTGGAAACA AGACCGAATG TGGTCTTCTA GGATTTGTAA ACCGTCTTGG AGGTGACTAT 
GCTGCCATCA GAAAGAAGTT CCCAGAACAT GACCTCACCA AGGTCTACAC TTTCAACTCT 
TCTCGTAAAT GCATGATGAC TGTTGTGCCA TATGCCGAGA ATGGCCAGAA TATCGGATAC 
CGTGTCTATT GCAAAGGAGC CTCTGAGATT GTGCTCGGCA GATGTACATA CTTGATCGGT 
TCTGACGGAA AGCCACATCA GCTTACTGGT GATCGATTGA AGGAGATTAC ATCTACTATC 
ATTCATGAGA TGGCTAACAG TGGTCTGAGA ACTATCTGTG TTGCTTATAA GACAATCATC 
AAGAAGGGAA CAAGAGATGT GGAGAAGACT GAGATCGAAT TCGCCGAAGA CTCTGATATC 
GATTGGGATG ACGAGGATGC CATGTACCAA AACTTCACCG GAATTGCTAT TTGCGGAATT 
CAAGATCCAG TTCGACCAGA GGTACCAGTC GCAATTTCAA AATGCAAAAA AGCTGGAATC 
ACTGTAAGAA TGGTTACTGG TGATAACATC ATGACCGCCA GAGCAATTGC AATGTCCTGT 
AAAATCTTGG AACCCGGAGA GGACTTTCTG GCTCTTGAAG GAAAGGAATT CAATGAGAGA 
ATCCGCGACG AAAATGGAAA AGTCTCTCAG GCAAAACTTG ATGAAATCTG GCCACGTCTT 
CGTGTGTTGG CTCGTGCTCA ACCTGCTGAT AAGTACACTC TTGTTAAGGG AATTATCGAC 
TCAAAAGCTA CTCCACAAAG AGAAATTGTT GCTGTAACTG GAGATGGTAC AAATGATGGT 
CCAGCTTTGA AGAAGGCTGA TGTTGGATTC GCTATGGGAA TTGCTGGAAC TGACGTTGCA 
AAGGAAGCTT CTGATATTAT TTTGACTGAT GATAATTTCA CTTCAATTGT TAAAGCAGTT 
ATGTGGGGAA GAAATGTGTA CGATTCCATC TCCAAGTTCT TGCAATTCCA ACTCACTGTC 
AATGTCGTCG CTGTCATCAC TGCCTTCGTT GGAGCTGTCA CAGTGTCTGA TTCACCATTG 
AAGGCAGTTC ACATGCTTTG GATTAATCTT ATCATGGACA CTCTTGCTTC ATTGGCCCTC 
GCAACTGAAC AGCCAACTGA TGAACTACTC GAAAGAAAAC CATACGGTCG CAAGAAGTCG 
CTCATCTCAA GAACAATGGT CAAGAATATT CTTTGTCATG CTCTTTACCA ACTTATCATT 
ATCTTTGTCA TCTTCTTCTA CGGAGACACC ATCTTTGGAA TCAAGACAGG ACTCTATGCA 
CCACTCTTCG CTCCACCATC ACAACATTTC ACTTTGGTTT TCAATGCATT CGTTATGATG 
ACTGTCTTCA ATGAGATCAA TGCTCGTAAG GTTCACGGAG AGCGTAACGT GTTTAAAGGA 
TTGGCATCTA ACCGAGTTTT CTGTGTCATT TGGGTTACAA CATTCATTGC TCAAATCATT 
ATTGTTCAAT TCGGAGGTGC CTGGTTCTCA ACTGCTCCAC TTACGCTTCA ACAATGGATT 
GTATGCCTCG TTCTTGGATT CTCAACTCTT ATCTGGGGAC AAATTGTGGC AACTATCCCA 
TCAAAGAAGC TACCAAAGGC CTGGAAAGTT GGAAAGGGAG AAGTTCAACC AGCCAATCTT 
CACATCAATG GAGACTACAA TGTCCGTGCT CGTTCCCGTG CGGTTACTCT TCGCAGATCT 
GGAAAGTCAC TCTGGGTTAG AGGAATGTTC ATTATTGGAA ACCATCTCCG CGTTCTTCGT 
GCTTTCGGAA TGGAAAAGAG TGAAAAGGCT GCCTTCGGAC GTACTGCTCC AGCAATGACG 
GCTGAAGCTG CTGAACGCTG GCGTGCCAGT TACCGCAAGT ACCGTCACCA GAAACATCAA 
GAGAAGAAAG CCACCGCGGA AACTGCTGAA TCTGTGAAAT CTGCCGACTG GGCCAAGGAA 
CAGAAGGAAA AGAAGAAGAC GTTCAAACAG ATCAAACAGG TTGCACGTGG AAAGAGTCTT 
GACAAGGATT CTAAGAAGCA TCACAAGAAG AGGAAGGATC AGACAAATGT CGATATGGAA 
GACATTGAAC TCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255409.1
CDS	1..3258
Translation	MSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001268480.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268480.2

ORF Insert Sequence:

ATGTCAACCA CCGAAGCACC ATCTGAAGGT CATGGAACTG CATGGATTGA AGGAGTTGCT 
ATTCTTCTCT GTGTTATTGT CGTCGTGCTG GTCACCGCTG TCAACGATTA TTCGAAAGAA 
CGACAGTTTA GAAGTTTGCA GGAAAAGATT GAAACTGGCC AAAAGTTCTC TGTTATTCGT 
AATGGGGAGG CTATTGATGT CCCCGTCTCT GATCTGGTAG TCGGTGACAT TGCACGTGTC 
AAATATGGTG ATCTTCTTCC TGCCGATGGA TTTTTGATTC AGAGTAATGA TTTGAAGATC 
GATGAATCAT CCCTCACTGG AGAATCAGAT CACATCAAAA AGAGCATTGA ATCCGATCCA 
GTTCTCCTTT CGGGAACATA TGCTATGGAA GGTTCCGGAA AAATGCTCAT CACTGCCGTA 
GGAGTCAACT CACAAACTGG TATTATCATG ACTCTTCTCG GGGCTGGAAA AGCTGGAATC 
GGAGATGATG ACTCTACATC AACATCTTCA AGTTCTTCTT CATCATCTAG TTCATCCGGA 
AGTTCGTCTA ACGGAAGCTC TGACAGCTCA AAATCTGGCG ATGACGACTT GACAGCCAAA 
TCAGTTCTGC AAGCTAAACT TTCCAAGTTG GCCCTTCAAA TCATCTATTG TGGAACTACA 
ATTGCCATCA TTGCACTTAT CGTCTTGGTC ACTCGATTCT GTCTTGACCA CTATGTTTTT 
GAAAAGAACG AGTTCTCACT GGTCGATATT CAAATGTTCG TCAAGTTCTT CATTATTGCT 
GTTACCATCT TGGTCATTTC CATACCTGAA GGTCTTCCAT TAGCTATTGC TCTTGCTCTT 
ACTTACTCTG TCAGAAAAAT GATGCACGAT AACAACTTGG TCCGTCACTT GGATGCATGT 
GAGACTATGG GAAATGCTAC TTCAATTTGC TCTGACAAAA CTGGAACTCT CACTACCAAC 
CGAATGACTG TTGTTCAATC ATACATCAAC GGAAACCATT ACACCAGTCA AGAAGCTCAA 
CCACATGGAG CCAACCTTCC AGGAAGCACT GGCCCAATTC TTATGGAGGC TATCTCAGTC 
AACTGTGCCT ATAACTCAAT GATTGTGGAG CCAACAAAAG CTGGAGAGCA AATTCAGCAA 
CTTGGAAACA AGACCGAATG TGGTCTTCTA GGATTTGTAA ACCGTCTTGG AGGTGACTAT 
GCTGCCATCA GAAAGAAGTT CCCAGAACAT GACCTCACCA AGGTCTACAC TTTCAACTCT 
TCTCGTAAAT GCATGATGAC TGTTGTGCCA TATGCCGAGA ATGGCCAGAA TATCGGATAC 
CGTGTCTATT GCAAAGGAGC CTCTGAGATT GTGCTCGGCA GATGTACATA CTTGATCGGT 
TCTGACGGAA AGCCACATCA GCTTACTGGT GATCGATTGA AGGAGATTAC ATCTACTATC 
ATTCATGAGA TGGCTAACAG TGGTCTGAGA ACTATCTGTG TTGCTTATAA GACAATCATC 
AAGAAGGGAA CAAGAGATGT GGAGAAGACT GAGATCGAAT TCGCCGAAGA CTCTGATATC 
GATTGGGATG ACGAGGATGC CATGTACCAA AACTTCACCG GAATTGCTAT TTGCGGAATT 
CAAGATCCAG TTCGACCAGA GGTACCAGTC GCAATTTCAA AATGCAAAAA AGCTGGAATC 
ACTGTAAGAA TGGTTACTGG TGATAACATC ATGACCGCCA GAGCAATTGC AATGTCCTGT 
AAAATCTTGG AACCCGGAGA GGACTTTCTG GCTCTTGAAG GAAAGGAATT CAATGAGAGA 
ATCCGCGACG AAAATGGAAA AGTCTCTCAG GCAAAACTTG ATGAAATCTG GCCACGTCTT 
CGTGTGTTGG CTCGTGCTCA ACCTGCTGAT AAGTACACTC TTGTTAAGGG AATTATCGAC 
TCAAAAGCTA CTCCACAAAG AGAAATTGTT GCTGTAACTG GAGATGGTAC AAATGATGGT 
CCAGCTTTGA AGAAGGCTGA TGTTGGATTC GCTATGGGAA TTGCTGGAAC TGACGTTGCA 
AAGGAAGCTT CTGATATTAT TTTGACTGAT GATAATTTCA CTTCAATTGT TAAAGCAGTT 
ATGTGGGGAA GAAATGTGTA CGATTCCATC TCCAAGTTCT TGCAATTCCA ACTCACTGTC 
AATGTCGTCG CTGTCATCAC TGCCTTCGTT GGAGCTGTCA CAGTGTCTGA TTCACCATTG 
AAGGCAGTTC ACATGCTTTG GATTAATCTT ATCATGGACA CTCTTGCTTC ATTGGCCCTC 
GCAACTGAAC AGCCAACTGA TGAACTACTC GAAAGAAAAC CATACGGTCG CAAGAAGTCG 
CTCATCTCAA GAACAATGGT CAAGAATATT CTTTGTCATG CTCTTTACCA ACTTATCATT 
ATCTTTGTCA TCTTCTTCTA CGGAGACACC ATCTTTGGAA TCAAGACAGG ACTCTATGCA 
CCACTCTTCG CTCCACCATC ACAACATTTC ACTTTGGTTT TCAATGCATT CGTTATGATG 
ACTGTCTTCA ATGAGATCAA TGCTCGTAAG GTTCACGGAG AGCGTAACGT GTTTAAAGGA 
TTGGCATCTA ACCGAGTTTT CTGTGTCATT TGGGTTACAA CATTCATTGC TCAAATCATT 
ATTGTTCAAT TCGGAGGTGC CTGGTTCTCA ACTGCTCCAC TTACGCTTCA ACAATGGATT 
GTATGCCTCG TTCTTGGATT CTCAACTCTT ATCTGGGGAC AAATTGTGGC AACTATCCCA 
TCAAAGAAGC TACCAAAGGC CTGGAAAGTT GGAAAGGGAG AAGTTCAACC AGCCAATCTT 
CACATCAATG GAGACTACAA TGTCCGTGCT CGTTCCCGTG CGGTTACTCT TCGCAGATCT 
GGAAAGTCAC TCTGGGTTAG AGGAATGTTC ATTATTGGAA ACCATCTCCG CGTTCTTCGT 
GCTTTCGGAA TGGAAAAGAG TGAAAAGGCT GCCTTCGGAC GTACTGCTCC AGCAATGACG 
GCTGAAGCTG CTGAACGCTG GCGTGCCAGT TACCGCAAGT ACCGTCACCA GAAACATCAA 
GAGAAGAAAG CCACCGCGGA AACTGCTGAA TCTGTGAAAT CTGCCGACTG GGCCAAGGAA 
CAGAAGGAAA AGAAGAAGAC GTTCAAACAG ATCAAACAGG TTGCACGTGG AAAGAGTCTT 
GACAAGGATT CTAAGAAGCA TCACAAGAAG AGGAAGGATC AGACAAATGT CGATATGGAA 
GACATTGAAC TCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001028256.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023427.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Price & Availability↑

CloneID	OCa344389
Clone ID Related Accession (Same CDS sequence)	NM_001028256.4 , NM_001028256.5
Accession Version	NM_001028256.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001028256.3. COMPLETENESS: incomplete on both ends.

ATGCCAATTA TTGCTATAAT TCTAGTTGTT TGTTTTATTG GACGTCTTAT TGTCTACTTT 
TGGAAGCGTG AGCCGAAAGG CGAAAATGGC AAAAAGGATG CAAAAGAATT TGGCTGCAGT 
CTTGGCGACC TTCGCGGACT CATGGAGGCT CGCGGAGCTG AGGCGATAGT TCGGCTGTCC 
ACAGAACACG AAGGCGTCGA GGGATTGTGC AAGAAATTGA AGACTGACTC GTTGGTTGGT 
CTCAATGGTG AACAAGCAGA TCTCGATCGA CGACGACATG TCTACGGAGC CAATACTATT 
CCACCAGCCA AAAGTAAAGG ATTCGTGCGG CTCGTTTTGG ATGCATGCAA GGACCCGACT 
CTTGTTATCC TGGTACTTTC CGGCTTCATC AATTTAGCTC TATCATTCTA CGAACCGACA 
TCGGCGGCCG AAGACGCAAC TCAACACCTT GTCAACGCGA CGACGGCGGC AATTCTTGCC 
AATGGAACCT TTATGTCAAC CACCGAAGCA CCATCTGAAG GTCATGGAAC TGCATGGATT 
GAAGGAGTTG CTATTCTTCT CTGTGTTATT GTCGTCGTGC TGGTCACCGC TGTCAACGAT 
TATTCGAAAG AACGACAGTT TAGAAGTTTG CAGGAAAAGA TTGAAACTGG CCAAAAGTTC 
TCTGTTATTC GTAATGGGGA GGCTATTGAT GTCCCCGTCT CTGATCTGGT AGTCGGTGAC 
ATTGCACGTG TCAAATATGG TGATCTTCTT CCTGCCGATG GATTTTTGAT TCAGAGTAAT 
GATTTGAAGA TCGATGAATC ATCCCTCACT GGAGAATCAG ATCACATCAA AAAGAGCATT 
GAATCCGATC CAGTTCTCCT TTCGGGAACA TATGCTATGG AAGGTTCCGG AAAAATGCTC 
ATCACTGCCG TAGGAGTCAA CTCACAAACT GGTATTATCA TGACTCTTCT CGGGGCTGGA 
AAAGCTGGAA TCGGAGATGA TGACTCTACA TCAACATCTT CAAGTTCTTC TTCATCATCT 
AGTTCATCCG GAAGTTCGTC TAACGGAAGC TCTGACAGCT CAAAATCTGG CGATGACGAC 
TTGACAGCCA AATCAGTTCT GCAAGCTAAA CTTTCCAAGT TGGCCCTTCA AATCATCTAT 
TGTGGAACTA CAATTGCCAT CATTGCACTT ATCGTCTTGG TCACTCGATT CTGTCTTGAC 
CACTATGTTT TTGAAAAGAA CGAGTTCTCA CTGGTCGATA TTCAAATGTT CGTCAAGTTC 
TTCATTATTG CTGTTACCAT CTTGGTCATT TCCATACCTG AAGGTCTTCC ATTAGCTATT 
GCTCTTGCTC TTACTTACTC TGTCAGAAAA ATGATGCACG ATAACAACTT GGTCCGTCAC 
TTGGATGCAT GTGAGACTAT GGGAAATGCT ACTTCAATTT GCTCTGACAA AACTGGAACT 
CTCACTACCA ACCGAATGAC TGTTGTTCAA TCATACATCA ACGGAAACCA TTACACCAGT 
CAAGAAGCTC AACCACATGG AGCCAACCTT CCAGGAAGCA CTGGCCCAAT TCTTATGGAG 
GCTATCTCAG TCAACTGTGC CTATAACTCA ATGATTGTGG AGCCAACAAA AGCTGGAGAG 
CAAATTCAGC AACTTGGAAA CAAGACCGAA TGTGGTCTTC TAGGATTTGT AAACCGTCTT 
GGAGGTGACT ATGCTGCCAT CAGAAAGAAG TTCCCAGAAC ATGACCTCAC CAAGGTCTAC 
ACTTTCAACT CTTCTCGTAA ATGCATGATG ACTGTTGTGC CATATGCCGA GAATGGCCAG 
AATATCGGAT ACCGTGTCTA TTGCAAAGGA GCCTCTGAGA TTGTGCTCGG CAGATGTACA 
TACTTGATCG GTTCTGACGG AAAGCCACAT CAGCTTACTG GTGATCGATT GAAGGAGATT 
ACATCTACTA TCATTCATGA GATGGCTAAC AGTGGTCTGA GAACTATCTG TGTTGCTTAT 
AAGACAATCA TCAAGAAGGG AACAAGAGAT GTGGAGAAGA CTGAGATCGA ATTCGCCGAA 
GACTCTGATA TCGATTGGGA TGACGAGGAT GCCATGTACC AAAACTTCAC CGGAATTGCT 
ATTTGCGGAA TTCAAGATCC AGTTCGACCA GAGGTACCAG TCGCAATTTC AAAATGCAAA 
AAAGCTGGAA TCACTGTAAG AATGGTTACT GGTGATAACA TCATGACCGC CAGAGCAATT 
GCAATGTCCT GTAAAATCTT GGAACCCGGA GAGGACTTTC TGGCTCTTGA AGGAAAGGAA 
TTCAATGAGA GAATCCGCGA CGAAAATGGA AAAGTCTCTC AGGCAAAACT TGATGAAATC 
TGGCCACGTC TTCGTGTGTT GGCTCGTGCT CAACCTGCTG ATAAGTACAC TCTTGTTAAG 
GGAATTATCG ACTCAAAAGC TACTCCACAA AGAGAAATTG TTGCTGTAAC TGGAGATGGT 
ACAAATGATG GTCCAGCTTT GAAGAAGGCT GATGTTGGAT TCGCTATGGG AATTGCTGGA 
ACTGACGTTG CAAAGGAAGC TTCTGATATT ATTTTGACTG ATGATAATTT CACTTCAATT 
GTTAAAGCAG TTATGTGGGG AAGAAATGTG TACGATTCCA TCTCCAAGTT CTTGCAATTC 
CAACTCACTG TCAATGTCGT CGCTGTCATC ACTGCCTTCG TTGGAGCTGT CACAGTGTCT 
GATTCACCAT TGAAGGCAGT TCACATGCTT TGGATTAATC TTATCATGGA CACTCTTGCT 
TCATTGGCCC TCGCAACTGA ACAGCCAACT GATGAACTAC TCGAAAGAAA ACCATACGGT 
CGCAAGAAGT CGCTCATCTC AAGAACAATG GTCAAGAATA TTCTTTGTCA TGCTCTTTAC 
CAACTTATCA TTATCTTTGT CATCTTCTTC TACGGAGACA CCATCTTTGG AATCAAGACA 
GGACTCTATG CACCACTCTT CGCTCCACCA TCACAACATT TCACTTTGGT TTTCAATGCA 
TTCGTTATGA TGACTGTCTT CAATGAGATC AATGCTCGTA AGGTTCACGG AGAGCGTAAC 
GTGTTTAAAG GATTGGCATC TAACCGAGTT TTCTGTGTCA TTTGGGTTAC AACATTCATT 
GCTCAAATCA TTATTGTTCA ATTCGGAGGT GCCTGGTTCT CAACTGCTCC ACTTACGCTT 
CAACAATGGA TTGTATGCCT CGTTCTTGGA TTCTCAACTC TTATCTGGGG ACAAATTGTG 
GCAACTATCC CATCAAAGAA GCTACCAAAG GCCTGGAAAG TTGGAAAGGG AGAAGTTCAA 
CCAGCCAATC TTCACATCAA TGGAGACTAC AATGTCCGTG CTCGTTCCCG TGCGGTTACT 
CTTCGCAGAT CTGGAAAGTC ACTCTGGGTT AGAGGAATGT TCATTATTGG AAACCATCTC 
CGCGTTCTTC GTGCTTTCGG AATGGAAAAG AGTGAAAAGG CTGCCTTCGG ACGTACTGCT 
CCAGCAATGA CGGCTGAAGC TGCTGAACGC TGGCGTGCCA GTTACCGCAA GTACCGTCAC 
CAGAAACATC AAGAGAAGAA AGCCACCGCG GAAACTGCTG AATCTGTGAA ATCTGCCGAC 
TGGGCCAAGG AACAGAAGGA AAAGAAGAAG ACGTTCAAAC AGATCAAACA GGTTGCACGT 
GGAAAGAGTC TTGACAAGGA TTCTAAGAAG CATCACAAGA AGAGGAAGGA TCAGACAAAT 
GTCGATATGG AAGACATTGA ACTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023427.1
CDS	1..3750
Translation	MPIIAIILVVCFIGRLIVYFWKREPKGENGKKDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001028256.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028256.4

ORF Insert Sequence:

ATGCCAATTA TTGCTATAAT TCTAGTTGTT TGTTTTATTG GACGTCTTAT TGTCTACTTT 
TGGAAGCGTG AGCCGAAAGG CGAAAATGGC AAAAAGGATG CAAAAGAATT TGGCTGCAGT 
CTTGGCGACC TTCGCGGACT CATGGAGGCT CGCGGAGCTG AGGCGATAGT TCGGCTGTCC 
ACAGAACACG AAGGCGTCGA GGGATTGTGC AAGAAATTGA AGACTGACTC GTTGGTTGGT 
CTCAATGGTG AACAAGCAGA TCTCGATCGA CGACGACATG TCTACGGAGC CAATACTATT 
CCACCAGCCA AAAGTAAAGG ATTCGTGCGG CTCGTTTTGG ATGCATGCAA GGACCCGACT 
CTTGTTATCC TGGTACTTTC CGGCTTCATC AATTTAGCTC TATCATTCTA CGAACCGACA 
TCGGCGGCCG AAGACGCAAC TCAACACCTT GTCAACGCGA CGACGGCGGC AATTCTTGCC 
AATGGAACCT TTATGTCAAC CACCGAAGCA CCATCTGAAG GTCATGGAAC TGCATGGATT 
GAAGGAGTTG CTATTCTTCT CTGTGTTATT GTCGTCGTGC TGGTCACCGC TGTCAACGAT 
TATTCGAAAG AACGACAGTT TAGAAGTTTG CAGGAAAAGA TTGAAACTGG CCAAAAGTTC 
TCTGTTATTC GTAATGGGGA GGCTATTGAT GTCCCCGTCT CTGATCTGGT AGTCGGTGAC 
ATTGCACGTG TCAAATATGG TGATCTTCTT CCTGCCGATG GATTTTTGAT TCAGAGTAAT 
GATTTGAAGA TCGATGAATC ATCCCTCACT GGAGAATCAG ATCACATCAA AAAGAGCATT 
GAATCCGATC CAGTTCTCCT TTCGGGAACA TATGCTATGG AAGGTTCCGG AAAAATGCTC 
ATCACTGCCG TAGGAGTCAA CTCACAAACT GGTATTATCA TGACTCTTCT CGGGGCTGGA 
AAAGCTGGAA TCGGAGATGA TGACTCTACA TCAACATCTT CAAGTTCTTC TTCATCATCT 
AGTTCATCCG GAAGTTCGTC TAACGGAAGC TCTGACAGCT CAAAATCTGG CGATGACGAC 
TTGACAGCCA AATCAGTTCT GCAAGCTAAA CTTTCCAAGT TGGCCCTTCA AATCATCTAT 
TGTGGAACTA CAATTGCCAT CATTGCACTT ATCGTCTTGG TCACTCGATT CTGTCTTGAC 
CACTATGTTT TTGAAAAGAA CGAGTTCTCA CTGGTCGATA TTCAAATGTT CGTCAAGTTC 
TTCATTATTG CTGTTACCAT CTTGGTCATT TCCATACCTG AAGGTCTTCC ATTAGCTATT 
GCTCTTGCTC TTACTTACTC TGTCAGAAAA ATGATGCACG ATAACAACTT GGTCCGTCAC 
TTGGATGCAT GTGAGACTAT GGGAAATGCT ACTTCAATTT GCTCTGACAA AACTGGAACT 
CTCACTACCA ACCGAATGAC TGTTGTTCAA TCATACATCA ACGGAAACCA TTACACCAGT 
CAAGAAGCTC AACCACATGG AGCCAACCTT CCAGGAAGCA CTGGCCCAAT TCTTATGGAG 
GCTATCTCAG TCAACTGTGC CTATAACTCA ATGATTGTGG AGCCAACAAA AGCTGGAGAG 
CAAATTCAGC AACTTGGAAA CAAGACCGAA TGTGGTCTTC TAGGATTTGT AAACCGTCTT 
GGAGGTGACT ATGCTGCCAT CAGAAAGAAG TTCCCAGAAC ATGACCTCAC CAAGGTCTAC 
ACTTTCAACT CTTCTCGTAA ATGCATGATG ACTGTTGTGC CATATGCCGA GAATGGCCAG 
AATATCGGAT ACCGTGTCTA TTGCAAAGGA GCCTCTGAGA TTGTGCTCGG CAGATGTACA 
TACTTGATCG GTTCTGACGG AAAGCCACAT CAGCTTACTG GTGATCGATT GAAGGAGATT 
ACATCTACTA TCATTCATGA GATGGCTAAC AGTGGTCTGA GAACTATCTG TGTTGCTTAT 
AAGACAATCA TCAAGAAGGG AACAAGAGAT GTGGAGAAGA CTGAGATCGA ATTCGCCGAA 
GACTCTGATA TCGATTGGGA TGACGAGGAT GCCATGTACC AAAACTTCAC CGGAATTGCT 
ATTTGCGGAA TTCAAGATCC AGTTCGACCA GAGGTACCAG TCGCAATTTC AAAATGCAAA 
AAAGCTGGAA TCACTGTAAG AATGGTTACT GGTGATAACA TCATGACCGC CAGAGCAATT 
GCAATGTCCT GTAAAATCTT GGAACCCGGA GAGGACTTTC TGGCTCTTGA AGGAAAGGAA 
TTCAATGAGA GAATCCGCGA CGAAAATGGA AAAGTCTCTC AGGCAAAACT TGATGAAATC 
TGGCCACGTC TTCGTGTGTT GGCTCGTGCT CAACCTGCTG ATAAGTACAC TCTTGTTAAG 
GGAATTATCG ACTCAAAAGC TACTCCACAA AGAGAAATTG TTGCTGTAAC TGGAGATGGT 
ACAAATGATG GTCCAGCTTT GAAGAAGGCT GATGTTGGAT TCGCTATGGG AATTGCTGGA 
ACTGACGTTG CAAAGGAAGC TTCTGATATT ATTTTGACTG ATGATAATTT CACTTCAATT 
GTTAAAGCAG TTATGTGGGG AAGAAATGTG TACGATTCCA TCTCCAAGTT CTTGCAATTC 
CAACTCACTG TCAATGTCGT CGCTGTCATC ACTGCCTTCG TTGGAGCTGT CACAGTGTCT 
GATTCACCAT TGAAGGCAGT TCACATGCTT TGGATTAATC TTATCATGGA CACTCTTGCT 
TCATTGGCCC TCGCAACTGA ACAGCCAACT GATGAACTAC TCGAAAGAAA ACCATACGGT 
CGCAAGAAGT CGCTCATCTC AAGAACAATG GTCAAGAATA TTCTTTGTCA TGCTCTTTAC 
CAACTTATCA TTATCTTTGT CATCTTCTTC TACGGAGACA CCATCTTTGG AATCAAGACA 
GGACTCTATG CACCACTCTT CGCTCCACCA TCACAACATT TCACTTTGGT TTTCAATGCA 
TTCGTTATGA TGACTGTCTT CAATGAGATC AATGCTCGTA AGGTTCACGG AGAGCGTAAC 
GTGTTTAAAG GATTGGCATC TAACCGAGTT TTCTGTGTCA TTTGGGTTAC AACATTCATT 
GCTCAAATCA TTATTGTTCA ATTCGGAGGT GCCTGGTTCT CAACTGCTCC ACTTACGCTT 
CAACAATGGA TTGTATGCCT CGTTCTTGGA TTCTCAACTC TTATCTGGGG ACAAATTGTG 
GCAACTATCC CATCAAAGAA GCTACCAAAG GCCTGGAAAG TTGGAAAGGG AGAAGTTCAA 
CCAGCCAATC TTCACATCAA TGGAGACTAC AATGTCCGTG CTCGTTCCCG TGCGGTTACT 
CTTCGCAGAT CTGGAAAGTC ACTCTGGGTT AGAGGAATGT TCATTATTGG AAACCATCTC 
CGCGTTCTTC GTGCTTTCGG AATGGAAAAG AGTGAAAAGG CTGCCTTCGG ACGTACTGCT 
CCAGCAATGA CGGCTGAAGC TGCTGAACGC TGGCGTGCCA GTTACCGCAA GTACCGTCAC 
CAGAAACATC AAGAGAAGAA AGCCACCGCG GAAACTGCTG AATCTGTGAA ATCTGCCGAC 
TGGGCCAAGG AACAGAAGGA AAAGAAGAAG ACGTTCAAAC AGATCAAACA GGTTGCACGT 
GGAAAGAGTC TTGACAAGGA TTCTAAGAAG CATCACAAGA AGAGGAAGGA TCAGACAAAT 
GTCGATATGG AAGACATTGA ACTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mca-1 ( NM_001028256.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023427.1 Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

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CloneID	OCa344389
Clone ID Related Accession (Same CDS sequence)	NM_001028256.4 , NM_001028256.5
Accession Version	NM_001028256.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-transporting ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001028256.4.

ATGCCAATTA TTGCTATAAT TCTAGTTGTT TGTTTTATTG GACGTCTTAT TGTCTACTTT 
TGGAAGCGTG AGCCGAAAGG CGAAAATGGC AAAAAGGATG CAAAAGAATT TGGCTGCAGT 
CTTGGCGACC TTCGCGGACT CATGGAGGCT CGCGGAGCTG AGGCGATAGT TCGGCTGTCC 
ACAGAACACG AAGGCGTCGA GGGATTGTGC AAGAAATTGA AGACTGACTC GTTGGTTGGT 
CTCAATGGTG AACAAGCAGA TCTCGATCGA CGACGACATG TCTACGGAGC CAATACTATT 
CCACCAGCCA AAAGTAAAGG ATTCGTGCGG CTCGTTTTGG ATGCATGCAA GGACCCGACT 
CTTGTTATCC TGGTACTTTC CGGCTTCATC AATTTAGCTC TATCATTCTA CGAACCGACA 
TCGGCGGCCG AAGACGCAAC TCAACACCTT GTCAACGCGA CGACGGCGGC AATTCTTGCC 
AATGGAACCT TTATGTCAAC CACCGAAGCA CCATCTGAAG GTCATGGAAC TGCATGGATT 
GAAGGAGTTG CTATTCTTCT CTGTGTTATT GTCGTCGTGC TGGTCACCGC TGTCAACGAT 
TATTCGAAAG AACGACAGTT TAGAAGTTTG CAGGAAAAGA TTGAAACTGG CCAAAAGTTC 
TCTGTTATTC GTAATGGGGA GGCTATTGAT GTCCCCGTCT CTGATCTGGT AGTCGGTGAC 
ATTGCACGTG TCAAATATGG TGATCTTCTT CCTGCCGATG GATTTTTGAT TCAGAGTAAT 
GATTTGAAGA TCGATGAATC ATCCCTCACT GGAGAATCAG ATCACATCAA AAAGAGCATT 
GAATCCGATC CAGTTCTCCT TTCGGGAACA TATGCTATGG AAGGTTCCGG AAAAATGCTC 
ATCACTGCCG TAGGAGTCAA CTCACAAACT GGTATTATCA TGACTCTTCT CGGGGCTGGA 
AAAGCTGGAA TCGGAGATGA TGACTCTACA TCAACATCTT CAAGTTCTTC TTCATCATCT 
AGTTCATCCG GAAGTTCGTC TAACGGAAGC TCTGACAGCT CAAAATCTGG CGATGACGAC 
TTGACAGCCA AATCAGTTCT GCAAGCTAAA CTTTCCAAGT TGGCCCTTCA AATCATCTAT 
TGTGGAACTA CAATTGCCAT CATTGCACTT ATCGTCTTGG TCACTCGATT CTGTCTTGAC 
CACTATGTTT TTGAAAAGAA CGAGTTCTCA CTGGTCGATA TTCAAATGTT CGTCAAGTTC 
TTCATTATTG CTGTTACCAT CTTGGTCATT TCCATACCTG AAGGTCTTCC ATTAGCTATT 
GCTCTTGCTC TTACTTACTC TGTCAGAAAA ATGATGCACG ATAACAACTT GGTCCGTCAC 
TTGGATGCAT GTGAGACTAT GGGAAATGCT ACTTCAATTT GCTCTGACAA AACTGGAACT 
CTCACTACCA ACCGAATGAC TGTTGTTCAA TCATACATCA ACGGAAACCA TTACACCAGT 
CAAGAAGCTC AACCACATGG AGCCAACCTT CCAGGAAGCA CTGGCCCAAT TCTTATGGAG 
GCTATCTCAG TCAACTGTGC CTATAACTCA ATGATTGTGG AGCCAACAAA AGCTGGAGAG 
CAAATTCAGC AACTTGGAAA CAAGACCGAA TGTGGTCTTC TAGGATTTGT AAACCGTCTT 
GGAGGTGACT ATGCTGCCAT CAGAAAGAAG TTCCCAGAAC ATGACCTCAC CAAGGTCTAC 
ACTTTCAACT CTTCTCGTAA ATGCATGATG ACTGTTGTGC CATATGCCGA GAATGGCCAG 
AATATCGGAT ACCGTGTCTA TTGCAAAGGA GCCTCTGAGA TTGTGCTCGG CAGATGTACA 
TACTTGATCG GTTCTGACGG AAAGCCACAT CAGCTTACTG GTGATCGATT GAAGGAGATT 
ACATCTACTA TCATTCATGA GATGGCTAAC AGTGGTCTGA GAACTATCTG TGTTGCTTAT 
AAGACAATCA TCAAGAAGGG AACAAGAGAT GTGGAGAAGA CTGAGATCGA ATTCGCCGAA 
GACTCTGATA TCGATTGGGA TGACGAGGAT GCCATGTACC AAAACTTCAC CGGAATTGCT 
ATTTGCGGAA TTCAAGATCC AGTTCGACCA GAGGTACCAG TCGCAATTTC AAAATGCAAA 
AAAGCTGGAA TCACTGTAAG AATGGTTACT GGTGATAACA TCATGACCGC CAGAGCAATT 
GCAATGTCCT GTAAAATCTT GGAACCCGGA GAGGACTTTC TGGCTCTTGA AGGAAAGGAA 
TTCAATGAGA GAATCCGCGA CGAAAATGGA AAAGTCTCTC AGGCAAAACT TGATGAAATC 
TGGCCACGTC TTCGTGTGTT GGCTCGTGCT CAACCTGCTG ATAAGTACAC TCTTGTTAAG 
GGAATTATCG ACTCAAAAGC TACTCCACAA AGAGAAATTG TTGCTGTAAC TGGAGATGGT 
ACAAATGATG GTCCAGCTTT GAAGAAGGCT GATGTTGGAT TCGCTATGGG AATTGCTGGA 
ACTGACGTTG CAAAGGAAGC TTCTGATATT ATTTTGACTG ATGATAATTT CACTTCAATT 
GTTAAAGCAG TTATGTGGGG AAGAAATGTG TACGATTCCA TCTCCAAGTT CTTGCAATTC 
CAACTCACTG TCAATGTCGT CGCTGTCATC ACTGCCTTCG TTGGAGCTGT CACAGTGTCT 
GATTCACCAT TGAAGGCAGT TCACATGCTT TGGATTAATC TTATCATGGA CACTCTTGCT 
TCATTGGCCC TCGCAACTGA ACAGCCAACT GATGAACTAC TCGAAAGAAA ACCATACGGT 
CGCAAGAAGT CGCTCATCTC AAGAACAATG GTCAAGAATA TTCTTTGTCA TGCTCTTTAC 
CAACTTATCA TTATCTTTGT CATCTTCTTC TACGGAGACA CCATCTTTGG AATCAAGACA 
GGACTCTATG CACCACTCTT CGCTCCACCA TCACAACATT TCACTTTGGT TTTCAATGCA 
TTCGTTATGA TGACTGTCTT CAATGAGATC AATGCTCGTA AGGTTCACGG AGAGCGTAAC 
GTGTTTAAAG GATTGGCATC TAACCGAGTT TTCTGTGTCA TTTGGGTTAC AACATTCATT 
GCTCAAATCA TTATTGTTCA ATTCGGAGGT GCCTGGTTCT CAACTGCTCC ACTTACGCTT 
CAACAATGGA TTGTATGCCT CGTTCTTGGA TTCTCAACTC TTATCTGGGG ACAAATTGTG 
GCAACTATCC CATCAAAGAA GCTACCAAAG GCCTGGAAAG TTGGAAAGGG AGAAGTTCAA 
CCAGCCAATC TTCACATCAA TGGAGACTAC AATGTCCGTG CTCGTTCCCG TGCGGTTACT 
CTTCGCAGAT CTGGAAAGTC ACTCTGGGTT AGAGGAATGT TCATTATTGG AAACCATCTC 
CGCGTTCTTC GTGCTTTCGG AATGGAAAAG AGTGAAAAGG CTGCCTTCGG ACGTACTGCT 
CCAGCAATGA CGGCTGAAGC TGCTGAACGC TGGCGTGCCA GTTACCGCAA GTACCGTCAC 
CAGAAACATC AAGAGAAGAA AGCCACCGCG GAAACTGCTG AATCTGTGAA ATCTGCCGAC 
TGGGCCAAGG AACAGAAGGA AAAGAAGAAG ACGTTCAAAC AGATCAAACA GGTTGCACGT 
GGAAAGAGTC TTGACAAGGA TTCTAAGAAG CATCACAAGA AGAGGAAGGA TCAGACAAAT 
GTCGATATGG AAGACATTGA ACTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023427.1
CDS	86..3835
Translation	MPIIAIILVVCFIGRLIVYFWKREPKGENGKKDAKEFGCSLGDLRGLMEARGAEAIVRLSTEHEGVEGLCKKLKTDSLVGLNGEQADLDRRRHVYGANTIPPAKSKGFVRLVLDACKDPTLVILVLSGFINLALSFYEPTSAAEDATQHLVNATTAAILANGTFMSTTEAPSEGHGTAWIEGVAILLCVIVVVLVTAVNDYSKERQFRSLQEKIETGQKFSVIRNGEAIDVPVSDLVVGDIARVKYGDLLPADGFLIQSNDLKIDESSLTGESDHIKKSIESDPVLLSGTYAMEGSGKMLITAVGVNSQTGIIMTLLGAGKAGIGDDDSTSTSSSSSSSSSSSGSSSNGSSDSSKSGDDDLTAKSVLQAKLSKLALQIIYCGTTIAIIALIVLVTRFCLDHYVFEKNEFSLVDIQMFVKFFIIAVTILVISIPEGLPLAIALALTYSVRKMMHDNNLVRHLDACETMGNATSICSDKTGTLTTNRMTVVQSYINGNHYTSQEAQPHGANLPGSTGPILMEAISVNCAYNSMIVEPTKAGEQIQQLGNKTECGLLGFVNRLGGDYAAIRKKFPEHDLTKVYTFNSSRKCMMTVVPYAENGQNIGYRVYCKGASEIVLGRCTYLIGSDGKPHQLTGDRLKEITSTIIHEMANSGLRTICVAYKTIIKKGTRDVEKTEIEFAEDSDIDWDDEDAMYQNFTGIAICGIQDPVRPEVPVAISKCKKAGITVRMVTGDNIMTARAIAMSCKILEPGEDFLALEGKEFNERIRDENGKVSQAKLDEIWPRLRVLARAQPADKYTLVKGIIDSKATPQREIVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVITAFVGAVTVSDSPLKAVHMLWINLIMDTLASLALATEQPTDELLERKPYGRKKSLISRTMVKNILCHALYQLIIIFVIFFYGDTIFGIKTGLYAPLFAPPSQHFTLVFNAFVMMTVFNEINARKVHGERNVFKGLASNRVFCVIWVTTFIAQIIIVQFGGAWFSTAPLTLQQWIVCLVLGFSTLIWGQIVATIPSKKLPKAWKVGKGEVQPANLHINGDYNVRARSRAVTLRRSGKSLWVRGMFIIGNHLRVLRAFGMEKSEKAAFGRTAPAMTAEAAERWRASYRKYRHQKHQEKKATAETAESVKSADWAKEQKEKKKTFKQIKQVARGKSLDKDSKKHHKKRKDQTNVDMEDIELN

Target ORF information:

RefSeq Version	NM_001028256.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-transporting ATPase (mca-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028256.5

ORF Insert Sequence:

ATGCCAATTA TTGCTATAAT TCTAGTTGTT TGTTTTATTG GACGTCTTAT TGTCTACTTT 
TGGAAGCGTG AGCCGAAAGG CGAAAATGGC AAAAAGGATG CAAAAGAATT TGGCTGCAGT 
CTTGGCGACC TTCGCGGACT CATGGAGGCT CGCGGAGCTG AGGCGATAGT TCGGCTGTCC 
ACAGAACACG AAGGCGTCGA GGGATTGTGC AAGAAATTGA AGACTGACTC GTTGGTTGGT 
CTCAATGGTG AACAAGCAGA TCTCGATCGA CGACGACATG TCTACGGAGC CAATACTATT 
CCACCAGCCA AAAGTAAAGG ATTCGTGCGG CTCGTTTTGG ATGCATGCAA GGACCCGACT 
CTTGTTATCC TGGTACTTTC CGGCTTCATC AATTTAGCTC TATCATTCTA CGAACCGACA 
TCGGCGGCCG AAGACGCAAC TCAACACCTT GTCAACGCGA CGACGGCGGC AATTCTTGCC 
AATGGAACCT TTATGTCAAC CACCGAAGCA CCATCTGAAG GTCATGGAAC TGCATGGATT 
GAAGGAGTTG CTATTCTTCT CTGTGTTATT GTCGTCGTGC TGGTCACCGC TGTCAACGAT 
TATTCGAAAG AACGACAGTT TAGAAGTTTG CAGGAAAAGA TTGAAACTGG CCAAAAGTTC 
TCTGTTATTC GTAATGGGGA GGCTATTGAT GTCCCCGTCT CTGATCTGGT AGTCGGTGAC 
ATTGCACGTG TCAAATATGG TGATCTTCTT CCTGCCGATG GATTTTTGAT TCAGAGTAAT 
GATTTGAAGA TCGATGAATC ATCCCTCACT GGAGAATCAG ATCACATCAA AAAGAGCATT 
GAATCCGATC CAGTTCTCCT TTCGGGAACA TATGCTATGG AAGGTTCCGG AAAAATGCTC 
ATCACTGCCG TAGGAGTCAA CTCACAAACT GGTATTATCA TGACTCTTCT CGGGGCTGGA 
AAAGCTGGAA TCGGAGATGA TGACTCTACA TCAACATCTT CAAGTTCTTC TTCATCATCT 
AGTTCATCCG GAAGTTCGTC TAACGGAAGC TCTGACAGCT CAAAATCTGG CGATGACGAC 
TTGACAGCCA AATCAGTTCT GCAAGCTAAA CTTTCCAAGT TGGCCCTTCA AATCATCTAT 
TGTGGAACTA CAATTGCCAT CATTGCACTT ATCGTCTTGG TCACTCGATT CTGTCTTGAC 
CACTATGTTT TTGAAAAGAA CGAGTTCTCA CTGGTCGATA TTCAAATGTT CGTCAAGTTC 
TTCATTATTG CTGTTACCAT CTTGGTCATT TCCATACCTG AAGGTCTTCC ATTAGCTATT 
GCTCTTGCTC TTACTTACTC TGTCAGAAAA ATGATGCACG ATAACAACTT GGTCCGTCAC 
TTGGATGCAT GTGAGACTAT GGGAAATGCT ACTTCAATTT GCTCTGACAA AACTGGAACT 
CTCACTACCA ACCGAATGAC TGTTGTTCAA TCATACATCA ACGGAAACCA TTACACCAGT 
CAAGAAGCTC AACCACATGG AGCCAACCTT CCAGGAAGCA CTGGCCCAAT TCTTATGGAG 
GCTATCTCAG TCAACTGTGC CTATAACTCA ATGATTGTGG AGCCAACAAA AGCTGGAGAG 
CAAATTCAGC AACTTGGAAA CAAGACCGAA TGTGGTCTTC TAGGATTTGT AAACCGTCTT 
GGAGGTGACT ATGCTGCCAT CAGAAAGAAG TTCCCAGAAC ATGACCTCAC CAAGGTCTAC 
ACTTTCAACT CTTCTCGTAA ATGCATGATG ACTGTTGTGC CATATGCCGA GAATGGCCAG 
AATATCGGAT ACCGTGTCTA TTGCAAAGGA GCCTCTGAGA TTGTGCTCGG CAGATGTACA 
TACTTGATCG GTTCTGACGG AAAGCCACAT CAGCTTACTG GTGATCGATT GAAGGAGATT 
ACATCTACTA TCATTCATGA GATGGCTAAC AGTGGTCTGA GAACTATCTG TGTTGCTTAT 
AAGACAATCA TCAAGAAGGG AACAAGAGAT GTGGAGAAGA CTGAGATCGA ATTCGCCGAA 
GACTCTGATA TCGATTGGGA TGACGAGGAT GCCATGTACC AAAACTTCAC CGGAATTGCT 
ATTTGCGGAA TTCAAGATCC AGTTCGACCA GAGGTACCAG TCGCAATTTC AAAATGCAAA 
AAAGCTGGAA TCACTGTAAG AATGGTTACT GGTGATAACA TCATGACCGC CAGAGCAATT 
GCAATGTCCT GTAAAATCTT GGAACCCGGA GAGGACTTTC TGGCTCTTGA AGGAAAGGAA 
TTCAATGAGA GAATCCGCGA CGAAAATGGA AAAGTCTCTC AGGCAAAACT TGATGAAATC 
TGGCCACGTC TTCGTGTGTT GGCTCGTGCT CAACCTGCTG ATAAGTACAC TCTTGTTAAG 
GGAATTATCG ACTCAAAAGC TACTCCACAA AGAGAAATTG TTGCTGTAAC TGGAGATGGT 
ACAAATGATG GTCCAGCTTT GAAGAAGGCT GATGTTGGAT TCGCTATGGG AATTGCTGGA 
ACTGACGTTG CAAAGGAAGC TTCTGATATT ATTTTGACTG ATGATAATTT CACTTCAATT 
GTTAAAGCAG TTATGTGGGG AAGAAATGTG TACGATTCCA TCTCCAAGTT CTTGCAATTC 
CAACTCACTG TCAATGTCGT CGCTGTCATC ACTGCCTTCG TTGGAGCTGT CACAGTGTCT 
GATTCACCAT TGAAGGCAGT TCACATGCTT TGGATTAATC TTATCATGGA CACTCTTGCT 
TCATTGGCCC TCGCAACTGA ACAGCCAACT GATGAACTAC TCGAAAGAAA ACCATACGGT 
CGCAAGAAGT CGCTCATCTC AAGAACAATG GTCAAGAATA TTCTTTGTCA TGCTCTTTAC 
CAACTTATCA TTATCTTTGT CATCTTCTTC TACGGAGACA CCATCTTTGG AATCAAGACA 
GGACTCTATG CACCACTCTT CGCTCCACCA TCACAACATT TCACTTTGGT TTTCAATGCA 
TTCGTTATGA TGACTGTCTT CAATGAGATC AATGCTCGTA AGGTTCACGG AGAGCGTAAC 
GTGTTTAAAG GATTGGCATC TAACCGAGTT TTCTGTGTCA TTTGGGTTAC AACATTCATT 
GCTCAAATCA TTATTGTTCA ATTCGGAGGT GCCTGGTTCT CAACTGCTCC ACTTACGCTT 
CAACAATGGA TTGTATGCCT CGTTCTTGGA TTCTCAACTC TTATCTGGGG ACAAATTGTG 
GCAACTATCC CATCAAAGAA GCTACCAAAG GCCTGGAAAG TTGGAAAGGG AGAAGTTCAA 
CCAGCCAATC TTCACATCAA TGGAGACTAC AATGTCCGTG CTCGTTCCCG TGCGGTTACT 
CTTCGCAGAT CTGGAAAGTC ACTCTGGGTT AGAGGAATGT TCATTATTGG AAACCATCTC 
CGCGTTCTTC GTGCTTTCGG AATGGAAAAG AGTGAAAAGG CTGCCTTCGG ACGTACTGCT 
CCAGCAATGA CGGCTGAAGC TGCTGAACGC TGGCGTGCCA GTTACCGCAA GTACCGTCAC 
CAGAAACATC AAGAGAAGAA AGCCACCGCG GAAACTGCTG AATCTGTGAA ATCTGCCGAC 
TGGGCCAAGG AACAGAAGGA AAAGAAGAAG ACGTTCAAAC AGATCAAACA GGTTGCACGT 
GGAAAGAGTC TTGACAAGGA TTCTAAGAAG CATCACAAGA AGAGGAAGGA TCAGACAAAT 
GTCGATATGG AAGACATTGA ACTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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mca-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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