wnk-1 cDNA ORF clone, Caenorhabditis elegans

The following wnk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa343069 NM_001307110.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OCa343070 NM_001307111.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OCa342172 NM_069202.6
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa342173 NM_182275.5
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343062 NM_001307103.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343063 NM_001307104.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343064 NM_001307105.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343065 NM_001307106.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343066 NM_001307107.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343067 NM_001307108.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343068 NM_001307109.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343980 NM_001268438.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343981 NM_001268443.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343982 NM_001268448.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343983 NM_001268446.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343984 NM_001268440.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343985 NM_001268447.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343986 NM_001268445.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343987 NM_001268439.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343988 NM_001268441.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343989 NM_001268442.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OCa343990 NM_001268444.2
Latest version!
Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OCa343986 NM_001268445.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343980 NM_001268438.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa342172 NM_069202.5 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343989 NM_001268442.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343982 NM_001268448.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343070 NM_001307111.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa342173 NM_182275.4 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343990 NM_001268444.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343068 NM_001307109.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343984 NM_001268440.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343062 NM_001307103.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343064 NM_001307105.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343985 NM_001268447.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343987 NM_001268439.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343066 NM_001307107.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343067 NM_001307108.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343069 NM_001307110.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OCa343988 NM_001268441.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343981 NM_001268443.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343063 NM_001307104.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343065 NM_001307106.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa343983 NM_001268446.1 Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa343069
    Clone ID Related Accession (Same CDS sequence) NM_001307110.2 , NM_001307110.1
    Accession Version NM_001307110.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307110.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGACAACGAA GGTCAACACC ACTGTAATTC CTTCCGATGT ATTGGCGACC
    CGAATGACAA TGTCTCAATC GTCAACCAAA TCAAGCAACG TCTCGGTATC ATCCCGTCAT
    CGCGACAATC AGTCCGCTCC GCCACGTCAT CATCACCATC AACCCCACCC TCCTCATCAT
    CCGCACCTCC AAAATCACTA TCATCCCCCA CAAAATCATA CGTCAGCCAC TGCTCCTTGT
    CCATCGGCTA TGGTTCAACT GCAAGCAGTG AGCAACAACA ACGTGAACCC TCTCCATCAG
    CCACCACATC CAGTTTCCTC TCAGATCCCG CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294039.1
    CDS7..588
    Translation

    Target ORF information:

    RefSeq Version NM_001307110.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307110.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGACAACGAA GGTCAACACC ACTGTAATTC CTTCCGATGT ATTGGCGACC
    CGAATGACAA TGTCTCAATC GTCAACCAAA TCAAGCAACG TCTCGGTATC ATCCCGTCAT
    CGCGACAATC AGTCCGCTCC GCCACGTCAT CATCACCATC AACCCCACCC TCCTCATCAT
    CCGCACCTCC AAAATCACTA TCATCCCCCA CAAAATCATA CGTCAGCCAC TGCTCCTTGT
    CCATCGGCTA TGGTTCAACT GCAAGCAGTG AGCAACAACA ACGTGAACCC TCTCCATCAG
    CCACCACATC CAGTTTCCTC TCAGATCCCG CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343069
    Clone ID Related Accession (Same CDS sequence) NM_001307110.2 , NM_001307110.1
    Accession Version NM_001307110.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGACAACGAA GGTCAACACC ACTGTAATTC CTTCCGATGT ATTGGCGACC
    CGAATGACAA TGTCTCAATC GTCAACCAAA TCAAGCAACG TCTCGGTATC ATCCCGTCAT
    CGCGACAATC AGTCCGCTCC GCCACGTCAT CATCACCATC AACCCCACCC TCCTCATCAT
    CCGCACCTCC AAAATCACTA TCATCCCCCA CAAAATCATA CGTCAGCCAC TGCTCCTTGT
    CCATCGGCTA TGGTTCAACT GCAAGCAGTG AGCAACAACA ACGTGAACCC TCTCCATCAG
    CCACCACATC CAGTTTCCTC TCAGATCCCG CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294039.1
    CDS1..582
    Translation

    Target ORF information:

    RefSeq Version NM_001307110.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGACAACGAA GGTCAACACC ACTGTAATTC CTTCCGATGT ATTGGCGACC
    CGAATGACAA TGTCTCAATC GTCAACCAAA TCAAGCAACG TCTCGGTATC ATCCCGTCAT
    CGCGACAATC AGTCCGCTCC GCCACGTCAT CATCACCATC AACCCCACCC TCCTCATCAT
    CCGCACCTCC AAAATCACTA TCATCCCCCA CAAAATCATA CGTCAGCCAC TGCTCCTTGT
    CCATCGGCTA TGGTTCAACT GCAAGCAGTG AGCAACAACA ACGTGAACCC TCTCCATCAG
    CCACCACATC CAGTTTCCTC TCAGATCCCG CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343070
    Clone ID Related Accession (Same CDS sequence) NM_001307111.2 , NM_001307111.1
    Accession Version NM_001307111.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307111.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGATTCCAGA CAACGAAGGT CAACACCACT GTAATTCCTT CCGATGTATT
    GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA AGCAACGTCT CGGTATCATC
    CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT CACCATCAAC CCCACCCTCC
    TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA AATCATACGT CAGCCACTGC
    TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC AACAACAACG TGAACCCTCT
    CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA CAGGCGTAAT AGAAAATGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294040.1
    CDS1..603
    Translation

    Target ORF information:

    RefSeq Version NM_001307111.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307111.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGATTCCAGA CAACGAAGGT CAACACCACT GTAATTCCTT CCGATGTATT
    GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA AGCAACGTCT CGGTATCATC
    CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT CACCATCAAC CCCACCCTCC
    TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA AATCATACGT CAGCCACTGC
    TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC AACAACAACG TGAACCCTCT
    CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA CAGGCGTAAT AGAAAATGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343070
    Clone ID Related Accession (Same CDS sequence) NM_001307111.2 , NM_001307111.1
    Accession Version NM_001307111.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGATTCCAGA CAACGAAGGT CAACACCACT GTAATTCCTT CCGATGTATT
    GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA AGCAACGTCT CGGTATCATC
    CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT CACCATCAAC CCCACCCTCC
    TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA AATCATACGT CAGCCACTGC
    TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC AACAACAACG TGAACCCTCT
    CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA CAGGCGTAAT AGAAAATGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294040.1
    CDS1..603
    Translation

    Target ORF information:

    RefSeq Version NM_001307111.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307111.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATTTGG AAAGCGAAGA TCCTGAGGTG ATCCAGATGA TTGTTCGTCA TCGAATGGAA 
    CAACACAAAC TTCTCGAAAA GCAACGTGTC GAGATTGAGC GTCTTCGTTC GAAAATTCGA
    GTTCCACGTG CAACTTCTGT GAATCCCGAA ATGATTGGAG ATGATGAAGC TGATACGACG
    CTTACTGCTC TTCAAAGTGC ATTAGGCAAT GCTTCATTGT CATTGCCAGC CAGTCCGCCA
    CCTAATACTG AGATTCCAGA CAACGAAGGT CAACACCACT GTAATTCCTT CCGATGTATT
    GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA AGCAACGTCT CGGTATCATC
    CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT CACCATCAAC CCCACCCTCC
    TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA AATCATACGT CAGCCACTGC
    TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC AACAACAACG TGAACCCTCT
    CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA CAGGCGTAAT AGAAAATGTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342172
    Clone ID Related Accession (Same CDS sequence) NM_069202.5 , NM_069202.6
    Accession Version NM_069202.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069202.4. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGACA ACGAAGGTCA ACACCACTGT AATTCCTTCC
    GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG CAACGTCTCG
    GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA CCATCAACCC
    CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA TCATACGTCA
    GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA CAACAACGTG
    AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_501603.3
    CDS1..5517
    Translation

    Target ORF information:

    RefSeq Version NM_069202.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_069202.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGACA ACGAAGGTCA ACACCACTGT AATTCCTTCC
    GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG CAACGTCTCG
    GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA CCATCAACCC
    CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA TCATACGTCA
    GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA CAACAACGTG
    AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342172
    Clone ID Related Accession (Same CDS sequence) NM_069202.5 , NM_069202.6
    Accession Version NM_069202.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069202.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGACA ACGAAGGTCA ACACCACTGT AATTCCTTCC
    GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG CAACGTCTCG
    GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA CCATCAACCC
    CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA TCATACGTCA
    GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA CAACAACGTG
    AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_501603.3
    CDS316..5832
    Translation

    Target ORF information:

    RefSeq Version NM_069202.6
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_069202.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGACA ACGAAGGTCA ACACCACTGT AATTCCTTCC
    GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG CAACGTCTCG
    GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA CCATCAACCC
    CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA TCATACGTCA
    GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA CAACAACGTG
    AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342173
    Clone ID Related Accession (Same CDS sequence) NM_182275.4 , NM_182275.5
    Accession Version NM_182275.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_182275.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGACAACG AAGGTCAACA CCACTGTAAT TCCTTCCGAT
    GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA CGTCTCGGTA
    TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA TCAACCCCAC
    CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA TACGTCAGCC
    ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA CAACGTGAAC
    CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872075.2
    CDS1..5034
    Translation

    Target ORF information:

    RefSeq Version NM_182275.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182275.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGACAACG AAGGTCAACA CCACTGTAAT TCCTTCCGAT
    GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA CGTCTCGGTA
    TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA TCAACCCCAC
    CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA TACGTCAGCC
    ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA CAACGTGAAC
    CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342173
    Clone ID Related Accession (Same CDS sequence) NM_182275.4 , NM_182275.5
    Accession Version NM_182275.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_182275.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGACAACG AAGGTCAACA CCACTGTAAT TCCTTCCGAT
    GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA CGTCTCGGTA
    TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA TCAACCCCAC
    CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA TACGTCAGCC
    ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA CAACGTGAAC
    CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872075.2
    CDS316..5349
    Translation

    Target ORF information:

    RefSeq Version NM_182275.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182275.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGACAACG AAGGTCAACA CCACTGTAAT TCCTTCCGAT
    GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA CGTCTCGGTA
    TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA TCAACCCCAC
    CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA TACGTCAGCC
    ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA CAACGTGAAC
    CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343062
    Clone ID Related Accession (Same CDS sequence) NM_001307103.1 , NM_001307103.2
    Accession Version NM_001307103.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGATTCCAGA CAACGAAGGT
    CAACACCACT GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC
    AACCAAATCA AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC
    ACGTCATCAT CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA
    TCCCCCACAA AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA
    AGCAGTGAGC AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA
    GATCCCGCCA CAGGCGTAAT AGAAAATGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294032.1
    CDS1..5553
    Translation

    Target ORF information:

    RefSeq Version NM_001307103.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307103.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGATTCCAGA CAACGAAGGT
    CAACACCACT GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC
    AACCAAATCA AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC
    ACGTCATCAT CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA
    TCCCCCACAA AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA
    AGCAGTGAGC AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA
    GATCCCGCCA CAGGCGTAAT AGAAAATGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343062
    Clone ID Related Accession (Same CDS sequence) NM_001307103.1 , NM_001307103.2
    Accession Version NM_001307103.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307103.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGATTCCAGA CAACGAAGGT
    CAACACCACT GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC
    AACCAAATCA AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC
    ACGTCATCAT CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA
    TCCCCCACAA AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA
    AGCAGTGAGC AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA
    GATCCCGCCA CAGGCGTAAT AGAAAATGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294032.1
    CDS316..5868
    Translation

    Target ORF information:

    RefSeq Version NM_001307103.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307103.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGATTCCAGA CAACGAAGGT
    CAACACCACT GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC
    AACCAAATCA AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC
    ACGTCATCAT CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA
    TCCCCCACAA AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA
    AGCAGTGAGC AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA
    GATCCCGCCA CAGGCGTAAT AGAAAATGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343063
    Clone ID Related Accession (Same CDS sequence) NM_001307104.1 , NM_001307104.2
    Accession Version NM_001307104.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA TTCCAGACAA CGAAGGTCAA
    CACCACTGTA ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC
    CAAATCAAGC AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG
    TCATCATCAC CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC
    CCCACAAAAT CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC
    AGTGAGCAAC AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT
    CCCGCCACAG GCGTAATAGA AAATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294033.1
    CDS1..5070
    Translation

    Target ORF information:

    RefSeq Version NM_001307104.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307104.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA TTCCAGACAA CGAAGGTCAA
    CACCACTGTA ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC
    CAAATCAAGC AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG
    TCATCATCAC CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC
    CCCACAAAAT CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC
    AGTGAGCAAC AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT
    CCCGCCACAG GCGTAATAGA AAATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343063
    Clone ID Related Accession (Same CDS sequence) NM_001307104.1 , NM_001307104.2
    Accession Version NM_001307104.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307104.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA TTCCAGACAA CGAAGGTCAA
    CACCACTGTA ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC
    CAAATCAAGC AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG
    TCATCATCAC CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC
    CCCACAAAAT CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC
    AGTGAGCAAC AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT
    CCCGCCACAG GCGTAATAGA AAATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294033.1
    CDS316..5385
    Translation

    Target ORF information:

    RefSeq Version NM_001307104.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307104.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA TTCCAGACAA CGAAGGTCAA
    CACCACTGTA ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC
    CAAATCAAGC AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG
    TCATCATCAC CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC
    CCCACAAAAT CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC
    AGTGAGCAAC AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT
    CCCGCCACAG GCGTAATAGA AAATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343064
    Clone ID Related Accession (Same CDS sequence) NM_001307105.1 , NM_001307105.2
    Accession Version NM_001307105.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294034.1
    CDS1..5544
    Translation

    Target ORF information:

    RefSeq Version NM_001307105.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343064
    Clone ID Related Accession (Same CDS sequence) NM_001307105.1 , NM_001307105.2
    Accession Version NM_001307105.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307105.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294034.1
    CDS316..5859
    Translation

    Target ORF information:

    RefSeq Version NM_001307105.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307105.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343065
    Clone ID Related Accession (Same CDS sequence) NM_001307106.1 , NM_001307106.2
    Accession Version NM_001307106.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ATTCCAGACA ACGAAGGTCA ACACCACTGT
    AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG
    CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA
    CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA
    TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA
    CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA
    GGCGTAATAG AAAATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294035.1
    CDS1..5061
    Translation

    Target ORF information:

    RefSeq Version NM_001307106.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ATTCCAGACA ACGAAGGTCA ACACCACTGT
    AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG
    CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA
    CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA
    TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA
    CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA
    GGCGTAATAG AAAATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343065
    Clone ID Related Accession (Same CDS sequence) NM_001307106.1 , NM_001307106.2
    Accession Version NM_001307106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307106.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ATTCCAGACA ACGAAGGTCA ACACCACTGT
    AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG
    CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA
    CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA
    TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA
    CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA
    GGCGTAATAG AAAATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294035.1
    CDS316..5376
    Translation

    Target ORF information:

    RefSeq Version NM_001307106.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ATTCCAGACA ACGAAGGTCA ACACCACTGT
    AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT CAATCGTCAA CCAAATCAAG
    CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC GCTCCGCCAC GTCATCATCA
    CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT CACTATCATC CCCCACAAAA
    TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT CAACTGCAAG CAGTGAGCAA
    CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT TCCTCTCAGA TCCCGCCACA
    GGCGTAATAG AAAATGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343066
    Clone ID Related Accession (Same CDS sequence) NM_001307107.2 , NM_001307107.1
    Accession Version NM_001307107.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307107.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGATTC CAGACAACGA AGGTCAACAC
    CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA
    ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA
    TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC
    ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT
    GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC
    GCCACAGGCG TAATAGAAAA TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294036.1
    CDS316..5862
    Translation

    Target ORF information:

    RefSeq Version NM_001307107.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGATTC CAGACAACGA AGGTCAACAC
    CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA
    ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA
    TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC
    ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT
    GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC
    GCCACAGGCG TAATAGAAAA TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343066
    Clone ID Related Accession (Same CDS sequence) NM_001307107.2 , NM_001307107.1
    Accession Version NM_001307107.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGATTC CAGACAACGA AGGTCAACAC
    CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA
    ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA
    TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC
    ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT
    GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC
    GCCACAGGCG TAATAGAAAA TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294036.1
    CDS1..5547
    Translation

    Target ORF information:

    RefSeq Version NM_001307107.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGATTC CAGACAACGA AGGTCAACAC
    CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA
    ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA
    TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC
    ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT
    GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC
    GCCACAGGCG TAATAGAAAA TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343067
    Clone ID Related Accession (Same CDS sequence) NM_001307108.2 , NM_001307108.1
    Accession Version NM_001307108.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307108.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294037.1
    CDS316..5379
    Translation

    Target ORF information:

    RefSeq Version NM_001307108.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307108.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343067
    Clone ID Related Accession (Same CDS sequence) NM_001307108.2 , NM_001307108.1
    Accession Version NM_001307108.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294037.1
    CDS1..5064
    Translation

    Target ORF information:

    RefSeq Version NM_001307108.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGATTCCAG ACAACGAAGG TCAACACCAC
    TGTAATTCCT TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC
    AAGCAACGTC TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA
    TCACCATCAA CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA
    AAATCATACG TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG
    CAACAACAAC GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC
    ACAGGCGTAA TAGAAAATGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343068
    Clone ID Related Accession (Same CDS sequence) NM_001307109.2 , NM_001307109.1
    Accession Version NM_001307109.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307109.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGATTCCA GACAACGAAG GTCAACACCA CTGTAATTCC
    TTCCGATGTA TTGGCGACCC GAATGACAAT GTCTCAATCG TCAACCAAAT CAAGCAACGT
    CTCGGTATCA TCCCGTCATC GCGACAATCA GTCCGCTCCG CCACGTCATC ATCACCATCA
    ACCCCACCCT CCTCATCATC CGCACCTCCA AAATCACTAT CATCCCCCAC AAAATCATAC
    GTCAGCCACT GCTCCTTGTC CATCGGCTAT GGTTCAACTG CAAGCAGTGA GCAACAACAA
    CGTGAACCCT CTCCATCAGC CACCACATCC AGTTTCCTCT CAGATCCCGC CACAGGCGTA
    ATAGAAAATG TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294038.1
    CDS316..5370
    Translation

    Target ORF information:

    RefSeq Version NM_001307109.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307109.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGATTCCA GACAACGAAG GTCAACACCA CTGTAATTCC
    TTCCGATGTA TTGGCGACCC GAATGACAAT GTCTCAATCG TCAACCAAAT CAAGCAACGT
    CTCGGTATCA TCCCGTCATC GCGACAATCA GTCCGCTCCG CCACGTCATC ATCACCATCA
    ACCCCACCCT CCTCATCATC CGCACCTCCA AAATCACTAT CATCCCCCAC AAAATCATAC
    GTCAGCCACT GCTCCTTGTC CATCGGCTAT GGTTCAACTG CAAGCAGTGA GCAACAACAA
    CGTGAACCCT CTCCATCAGC CACCACATCC AGTTTCCTCT CAGATCCCGC CACAGGCGTA
    ATAGAAAATG TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343068
    Clone ID Related Accession (Same CDS sequence) NM_001307109.2 , NM_001307109.1
    Accession Version NM_001307109.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGATTCCA GACAACGAAG GTCAACACCA CTGTAATTCC
    TTCCGATGTA TTGGCGACCC GAATGACAAT GTCTCAATCG TCAACCAAAT CAAGCAACGT
    CTCGGTATCA TCCCGTCATC GCGACAATCA GTCCGCTCCG CCACGTCATC ATCACCATCA
    ACCCCACCCT CCTCATCATC CGCACCTCCA AAATCACTAT CATCCCCCAC AAAATCATAC
    GTCAGCCACT GCTCCTTGTC CATCGGCTAT GGTTCAACTG CAAGCAGTGA GCAACAACAA
    CGTGAACCCT CTCCATCAGC CACCACATCC AGTTTCCTCT CAGATCCCGC CACAGGCGTA
    ATAGAAAATG TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294038.1
    CDS1..5055
    Translation

    Target ORF information:

    RefSeq Version NM_001307109.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001307109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGGGTC AAATGGATTT GGAAAGCGAA GATCCTGAGG TGATCCAGAT GATTGTTCGT
    CATCGAATGG AACAACACAA ACTTCTCGAA AAGCAACGTG TCGAGATTGA GCGTCTTCGT
    TCGAAAATTC GAGTTCCACG TGCAACTTCT GTGAATCCCG AAATGATTGG AGATGATGAA
    GCTGATACGA CGCTTACTGC TCTTCAAAGT GCATTAGGCA ATGCTTCATT GTCATTGCCA
    GCCAGTCCGC CACCTAATAC TGAGATTCCA GACAACGAAG GTCAACACCA CTGTAATTCC
    TTCCGATGTA TTGGCGACCC GAATGACAAT GTCTCAATCG TCAACCAAAT CAAGCAACGT
    CTCGGTATCA TCCCGTCATC GCGACAATCA GTCCGCTCCG CCACGTCATC ATCACCATCA
    ACCCCACCCT CCTCATCATC CGCACCTCCA AAATCACTAT CATCCCCCAC AAAATCATAC
    GTCAGCCACT GCTCCTTGTC CATCGGCTAT GGTTCAACTG CAAGCAGTGA GCAACAACAA
    CGTGAACCCT CTCCATCAGC CACCACATCC AGTTTCCTCT CAGATCCCGC CACAGGCGTA
    ATAGAAAATG TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343980
    Clone ID Related Accession (Same CDS sequence) NM_001268438.2 , NM_001268438.1
    Accession Version NM_001268438.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268438.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGATT CCAGACAACG AAGGTCAACA CCACTGTAAT
    TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA
    CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA
    TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA
    TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA
    CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC
    GTAATAGAAA ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255367.1
    CDS316..5853
    Translation

    Target ORF information:

    RefSeq Version NM_001268438.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268438.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGATT CCAGACAACG AAGGTCAACA CCACTGTAAT
    TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA
    CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA
    TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA
    TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA
    CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC
    GTAATAGAAA ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343980
    Clone ID Related Accession (Same CDS sequence) NM_001268438.2 , NM_001268438.1
    Accession Version NM_001268438.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGATT CCAGACAACG AAGGTCAACA CCACTGTAAT
    TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA
    CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA
    TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA
    TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA
    CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC
    GTAATAGAAA ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255367.1
    CDS1..5538
    Translation

    Target ORF information:

    RefSeq Version NM_001268438.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGGTCAGT
    GGAGTATCAC ACTCGGCTTC ACCTTCAACG GTAGTCGAGT CGCTCACCTC AATGACTCCA
    CAAACAATTC CATTGGCTTG TCAAACTGTG CCCGCATCGA TCGGTCAAGC TCCAGCAGTC
    ATCGCAGCTG CACACGCGGC GTCATTAATT CCAAATGCAA GTGTTCCACA GTCACCTAGT
    CGTCTTGATG CCGAAACTGG ACTTGCCGGT CTTCACGAGA AGCTTGAAGC GCTGAAAATG
    GAACAGGATC GACGAGAAGA TATGGGAGAT GATGCGATTG GCACAACAAC CACAGACGGA
    AAGGACGAGA TACCGATTGA TACGTTAAAA GGGCTCGCGG AAGCATTAGG AAAAGTTATT
    CATGCGGATG GTAGAGAAAC AACACCAATG CCACCGGATC ATCCGGATTT AACAGATGCT
    TCAACGCAAC AACTCATCTC ACCATCTAAC CCTGATGTTT TGACAACGAT GTCGTCGGCT
    GTCGAAGGAT CAGCATCATC CACAATGATA GAAGACATTG ATGCTTCAAC ATCAGCGGTA
    GACGCGTCAA TGATGAATTC GATGCCGCCA GGAGCTCAGA ACTCTACAGA TCAGATACCA
    GCAGCGATGA CACTGAGTAT GGATCAAGAA TGTGCTCAAT CGATGACGTC ATCGATAACA
    AGAAACACGA CAGGCACCAA ATTGGCGACT TTTGAGAATC TTGAAACAGC TCTATCTTCC
    ACACTCGGAA CACACATTCG TCAACCGAAT GCACCGTCTT CTCGAGATGA GACGACGGCT
    CCGATGACAC CAAGTTTCAC AAATGAAAGA ATTGGTGGTG GTGGAGGTGG TGGAGCAACA
    TCATTTAGTA TAGGAACACC GCCCAGCCAT AGTCCGTTCC CTGTTTCGGA GTGTGATTAT
    GATTTGAAGG GTCAAATGGA TTTGGAAAGC GAAGATCCTG AGGTGATCCA GATGATTGTT
    CGTCATCGAA TGGAACAACA CAAACTTCTC GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT
    CGTTCGAAAA TTCGAGTTCC ACGTGCAACT TCTGTGAATC CCGAAATGAT TGGAGATGAT
    GAAGCTGATA CGACGCTTAC TGCTCTTCAA AGTGCATTAG GCAATGCTTC ATTGTCATTG
    CCAGCCAGTC CGCCACCTAA TACTGAGATT CCAGACAACG AAGGTCAACA CCACTGTAAT
    TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA TCGTCAACCA AATCAAGCAA
    CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT CCGCCACGTC ATCATCACCA
    TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC TATCATCCCC CACAAAATCA
    TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA CTGCAAGCAG TGAGCAACAA
    CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC TCTCAGATCC CGCCACAGGC
    GTAATAGAAA ATGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343981
    Clone ID Related Accession (Same CDS sequence) NM_001268443.1 , NM_001268443.2
    Accession Version NM_001268443.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGACAA CGAAGGTCAA CACCACTGTA
    ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC CAAATCAAGC
    AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG TCATCATCAC
    CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC CCCACAAAAT
    CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC AGTGAGCAAC
    AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT CCCGCCACAG
    GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255372.1
    CDS1..5526
    Translation

    Target ORF information:

    RefSeq Version NM_001268443.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGACAA CGAAGGTCAA CACCACTGTA
    ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC CAAATCAAGC
    AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG TCATCATCAC
    CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC CCCACAAAAT
    CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC AGTGAGCAAC
    AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT CCCGCCACAG
    GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343981
    Clone ID Related Accession (Same CDS sequence) NM_001268443.1 , NM_001268443.2
    Accession Version NM_001268443.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268443.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGACAA CGAAGGTCAA CACCACTGTA
    ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC CAAATCAAGC
    AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG TCATCATCAC
    CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC CCCACAAAAT
    CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC AGTGAGCAAC
    AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT CCCGCCACAG
    GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255372.1
    CDS316..5841
    Translation

    Target ORF information:

    RefSeq Version NM_001268443.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGCAACA ACTCATCTCA CCATCTAACC CTGATGTTTT GACAACGATG
    TCGTCGGCTG TCGAAGGATC AGCATCATCC ACAATGATAG AAGACATTGA TGCTTCAACA
    TCAGCGGTAG ACGCGTCAAT GATGAATTCG ATGCCGCCAG GAGCTCAGAA CTCTACAGAT
    CAGATACCAG CAGCGATGAC ACTGAGTATG GATCAAGAAT GTGCTCAATC GATGACGTCA
    TCGATAACAA GAAACACGAC AGGCACCAAA TTGGCGACTT TTGAGAATCT TGAAACAGCT
    CTATCTTCCA CACTCGGAAC ACACATTCGT CAACCGAATG CACCGTCTTC TCGAGATGAG
    ACGACGGCTC CGATGACACC AAGTTTCACA AATGAAAGAA TTGGTGGTGG TGGAGGTGGT
    GGAGCAACAT CATTTAGTAT AGGAACACCG CCCAGCCATA GTCCGTTCCC TGTTTCGGAG
    TGTGATTATG ATTTGAAGGG TCAAATGGAT TTGGAAAGCG AAGATCCTGA GGTGATCCAG
    ATGATTGTTC GTCATCGAAT GGAACAACAC AAACTTCTCG AAAAGCAACG TGTCGAGATT
    GAGCGTCTTC GTTCGAAAAT TCGAGTTCCA CGTGCAACTT CTGTGAATCC CGAAATGATT
    GGAGATGATG AAGCTGATAC GACGCTTACT GCTCTTCAAA GTGCATTAGG CAATGCTTCA
    TTGTCATTGC CAGCCAGTCC GCCACCTAAT ACTGAGACAA CGAAGGTCAA CACCACTGTA
    ATTCCTTCCG ATGTATTGGC GACCCGAATG ACAATGTCTC AATCGTCAAC CAAATCAAGC
    AACGTCTCGG TATCATCCCG TCATCGCGAC AATCAGTCCG CTCCGCCACG TCATCATCAC
    CATCAACCCC ACCCTCCTCA TCATCCGCAC CTCCAAAATC ACTATCATCC CCCACAAAAT
    CATACGTCAG CCACTGCTCC TTGTCCATCG GCTATGGTTC AACTGCAAGC AGTGAGCAAC
    AACAACGTGA ACCCTCTCCA TCAGCCACCA CATCCAGTTT CCTCTCAGAT CCCGCCACAG
    GCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343982
    Clone ID Related Accession (Same CDS sequence) NM_001268448.2 , NM_001268448.1
    Accession Version NM_001268448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268448.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGGTCAG TGGAGTATCA CACTCGGCTT CACCTTCAAC GGTAGTCGAG
    TCGCTCACCT CAATGACTCC ACAAACAATT CCATTGGCTT GTCAAACTGT GCCCGCATCG
    ATCGGTCAAG CTCCAGCAGT CATCGCAGCT GCACACGCGG CGTCATTAAT TCCAAATGCA
    AGTGTTCCAC AGTCACCTAG TCGTCTTGAT GCCGAAACTG GACTTGCCGG TCTTCACGAG
    AAGCTTGAAG CGCTGAAAAT GGAACAGGAT CGACGAGAAG ATATGGGAGA TGATGCGATT
    GGCACAACAA CCACAGACGG AAAGGACGAG ATACCGATTG ATACGTTAAA AGGGCTCGCG
    GAAGCATTAG GAAAAGTTAT TCATGCGGAT GGTAGAGAAA CAACACCAAT GCCACCGGAT
    CATCCGGATT TAACAGATGC TTCAACGCAA CAACTCATCT CACCATCTAA CCCTGATGTT
    TTGACAACGA TGTCGTCGGC TGTCGAAGGA TCAGCATCAT CCACAATGAT AGAAGACATT
    GATGCTTCAA CATCAGCGGT AGACGCGTCA ATGATGAATT CGATGCCGCC AGGAGCTCAG
    AACTCTACAG ATCAGATACC AGCAGCGATG ACACTGAGTA TGGATCAAGA ATGTGCTCAA
    TCGATGACGT CATCGATAAC AAGAAACACG ACAGGCACCA AATTGGCGAC TTTTGAGAAT
    CTTGAAACAG CTCTATCTTC CACACTCGGA ACACACATTC GTCAACCGAA TGCACCGTCT
    TCTCGAGATG AGACGACGGC TCCGATGACA CCAAGTTTCA CAAATGAAAG AATTGGTGGT
    GGTGGAGGTG GTGGAGCAAC ATCATTTAGT ATAGGAACAC CGCCCAGCCA TAGTCCGTTC
    CCTGTTTCGG AGTGTGATTA TGATTTGAAG GGTCAAATGG ATTTGGAAAG CGAAGATCCT
    GAGGTGATCC AGATGATTGT TCGTCATCGA ATGGAACAAC ACAAACTTCT CGAAAAGCAA
    CGTGTCGAGA TTGAGCGTCT TCGTTCGAAA ATTCGAGTTC CACGTGCAAC TTCTGTGAAT
    CCCGAAATGA TTGGAGATGA TGAAGCTGAT ACGACGCTTA CTGCTCTTCA AAGTGCATTA
    GGCAATGCTT CATTGTCATT GCCAGCCAGT CCGCCACCTA ATACTGAGAC AACGAAGGTC
    AACACCACTG TAATTCCTTC CGATGTATTG GCGACCCGAA TGACAATGTC TCAATCGTCA
    ACCAAATCAA GCAACGTCTC GGTATCATCC CGTCATCGCG ACAATCAGTC CGCTCCGCCA
    CGTCATCATC ACCATCAACC CCACCCTCCT CATCATCCGC ACCTCCAAAA TCACTATCAT
    CCCCCACAAA ATCATACGTC AGCCACTGCT CCTTGTCCAT CGGCTATGGT TCAACTGCAA
    GCAGTGAGCA ACAACAACGT GAACCCTCTC CATCAGCCAC CACATCCAGT TTCCTCTCAG
    ATCCCGCCAC AGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255377.1
    CDS1..5358
    Translation

    Target ORF information:

    RefSeq Version NM_001268448.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGGTCAG TGGAGTATCA CACTCGGCTT CACCTTCAAC GGTAGTCGAG
    TCGCTCACCT CAATGACTCC ACAAACAATT CCATTGGCTT GTCAAACTGT GCCCGCATCG
    ATCGGTCAAG CTCCAGCAGT CATCGCAGCT GCACACGCGG CGTCATTAAT TCCAAATGCA
    AGTGTTCCAC AGTCACCTAG TCGTCTTGAT GCCGAAACTG GACTTGCCGG TCTTCACGAG
    AAGCTTGAAG CGCTGAAAAT GGAACAGGAT CGACGAGAAG ATATGGGAGA TGATGCGATT
    GGCACAACAA CCACAGACGG AAAGGACGAG ATACCGATTG ATACGTTAAA AGGGCTCGCG
    GAAGCATTAG GAAAAGTTAT TCATGCGGAT GGTAGAGAAA CAACACCAAT GCCACCGGAT
    CATCCGGATT TAACAGATGC TTCAACGCAA CAACTCATCT CACCATCTAA CCCTGATGTT
    TTGACAACGA TGTCGTCGGC TGTCGAAGGA TCAGCATCAT CCACAATGAT AGAAGACATT
    GATGCTTCAA CATCAGCGGT AGACGCGTCA ATGATGAATT CGATGCCGCC AGGAGCTCAG
    AACTCTACAG ATCAGATACC AGCAGCGATG ACACTGAGTA TGGATCAAGA ATGTGCTCAA
    TCGATGACGT CATCGATAAC AAGAAACACG ACAGGCACCA AATTGGCGAC TTTTGAGAAT
    CTTGAAACAG CTCTATCTTC CACACTCGGA ACACACATTC GTCAACCGAA TGCACCGTCT
    TCTCGAGATG AGACGACGGC TCCGATGACA CCAAGTTTCA CAAATGAAAG AATTGGTGGT
    GGTGGAGGTG GTGGAGCAAC ATCATTTAGT ATAGGAACAC CGCCCAGCCA TAGTCCGTTC
    CCTGTTTCGG AGTGTGATTA TGATTTGAAG GGTCAAATGG ATTTGGAAAG CGAAGATCCT
    GAGGTGATCC AGATGATTGT TCGTCATCGA ATGGAACAAC ACAAACTTCT CGAAAAGCAA
    CGTGTCGAGA TTGAGCGTCT TCGTTCGAAA ATTCGAGTTC CACGTGCAAC TTCTGTGAAT
    CCCGAAATGA TTGGAGATGA TGAAGCTGAT ACGACGCTTA CTGCTCTTCA AAGTGCATTA
    GGCAATGCTT CATTGTCATT GCCAGCCAGT CCGCCACCTA ATACTGAGAC AACGAAGGTC
    AACACCACTG TAATTCCTTC CGATGTATTG GCGACCCGAA TGACAATGTC TCAATCGTCA
    ACCAAATCAA GCAACGTCTC GGTATCATCC CGTCATCGCG ACAATCAGTC CGCTCCGCCA
    CGTCATCATC ACCATCAACC CCACCCTCCT CATCATCCGC ACCTCCAAAA TCACTATCAT
    CCCCCACAAA ATCATACGTC AGCCACTGCT CCTTGTCCAT CGGCTATGGT TCAACTGCAA
    GCAGTGAGCA ACAACAACGT GAACCCTCTC CATCAGCCAC CACATCCAGT TTCCTCTCAG
    ATCCCGCCAC AGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343982
    Clone ID Related Accession (Same CDS sequence) NM_001268448.2 , NM_001268448.1
    Accession Version NM_001268448.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGGTCAG TGGAGTATCA CACTCGGCTT CACCTTCAAC GGTAGTCGAG
    TCGCTCACCT CAATGACTCC ACAAACAATT CCATTGGCTT GTCAAACTGT GCCCGCATCG
    ATCGGTCAAG CTCCAGCAGT CATCGCAGCT GCACACGCGG CGTCATTAAT TCCAAATGCA
    AGTGTTCCAC AGTCACCTAG TCGTCTTGAT GCCGAAACTG GACTTGCCGG TCTTCACGAG
    AAGCTTGAAG CGCTGAAAAT GGAACAGGAT CGACGAGAAG ATATGGGAGA TGATGCGATT
    GGCACAACAA CCACAGACGG AAAGGACGAG ATACCGATTG ATACGTTAAA AGGGCTCGCG
    GAAGCATTAG GAAAAGTTAT TCATGCGGAT GGTAGAGAAA CAACACCAAT GCCACCGGAT
    CATCCGGATT TAACAGATGC TTCAACGCAA CAACTCATCT CACCATCTAA CCCTGATGTT
    TTGACAACGA TGTCGTCGGC TGTCGAAGGA TCAGCATCAT CCACAATGAT AGAAGACATT
    GATGCTTCAA CATCAGCGGT AGACGCGTCA ATGATGAATT CGATGCCGCC AGGAGCTCAG
    AACTCTACAG ATCAGATACC AGCAGCGATG ACACTGAGTA TGGATCAAGA ATGTGCTCAA
    TCGATGACGT CATCGATAAC AAGAAACACG ACAGGCACCA AATTGGCGAC TTTTGAGAAT
    CTTGAAACAG CTCTATCTTC CACACTCGGA ACACACATTC GTCAACCGAA TGCACCGTCT
    TCTCGAGATG AGACGACGGC TCCGATGACA CCAAGTTTCA CAAATGAAAG AATTGGTGGT
    GGTGGAGGTG GTGGAGCAAC ATCATTTAGT ATAGGAACAC CGCCCAGCCA TAGTCCGTTC
    CCTGTTTCGG AGTGTGATTA TGATTTGAAG GGTCAAATGG ATTTGGAAAG CGAAGATCCT
    GAGGTGATCC AGATGATTGT TCGTCATCGA ATGGAACAAC ACAAACTTCT CGAAAAGCAA
    CGTGTCGAGA TTGAGCGTCT TCGTTCGAAA ATTCGAGTTC CACGTGCAAC TTCTGTGAAT
    CCCGAAATGA TTGGAGATGA TGAAGCTGAT ACGACGCTTA CTGCTCTTCA AAGTGCATTA
    GGCAATGCTT CATTGTCATT GCCAGCCAGT CCGCCACCTA ATACTGAGAC AACGAAGGTC
    AACACCACTG TAATTCCTTC CGATGTATTG GCGACCCGAA TGACAATGTC TCAATCGTCA
    ACCAAATCAA GCAACGTCTC GGTATCATCC CGTCATCGCG ACAATCAGTC CGCTCCGCCA
    CGTCATCATC ACCATCAACC CCACCCTCCT CATCATCCGC ACCTCCAAAA TCACTATCAT
    CCCCCACAAA ATCATACGTC AGCCACTGCT CCTTGTCCAT CGGCTATGGT TCAACTGCAA
    GCAGTGAGCA ACAACAACGT GAACCCTCTC CATCAGCCAC CACATCCAGT TTCCTCTCAG
    ATCCCGCCAC AGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255377.1
    CDS1..5358
    Translation

    Target ORF information:

    RefSeq Version NM_001268448.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGGTCAG TGGAGTATCA CACTCGGCTT CACCTTCAAC GGTAGTCGAG
    TCGCTCACCT CAATGACTCC ACAAACAATT CCATTGGCTT GTCAAACTGT GCCCGCATCG
    ATCGGTCAAG CTCCAGCAGT CATCGCAGCT GCACACGCGG CGTCATTAAT TCCAAATGCA
    AGTGTTCCAC AGTCACCTAG TCGTCTTGAT GCCGAAACTG GACTTGCCGG TCTTCACGAG
    AAGCTTGAAG CGCTGAAAAT GGAACAGGAT CGACGAGAAG ATATGGGAGA TGATGCGATT
    GGCACAACAA CCACAGACGG AAAGGACGAG ATACCGATTG ATACGTTAAA AGGGCTCGCG
    GAAGCATTAG GAAAAGTTAT TCATGCGGAT GGTAGAGAAA CAACACCAAT GCCACCGGAT
    CATCCGGATT TAACAGATGC TTCAACGCAA CAACTCATCT CACCATCTAA CCCTGATGTT
    TTGACAACGA TGTCGTCGGC TGTCGAAGGA TCAGCATCAT CCACAATGAT AGAAGACATT
    GATGCTTCAA CATCAGCGGT AGACGCGTCA ATGATGAATT CGATGCCGCC AGGAGCTCAG
    AACTCTACAG ATCAGATACC AGCAGCGATG ACACTGAGTA TGGATCAAGA ATGTGCTCAA
    TCGATGACGT CATCGATAAC AAGAAACACG ACAGGCACCA AATTGGCGAC TTTTGAGAAT
    CTTGAAACAG CTCTATCTTC CACACTCGGA ACACACATTC GTCAACCGAA TGCACCGTCT
    TCTCGAGATG AGACGACGGC TCCGATGACA CCAAGTTTCA CAAATGAAAG AATTGGTGGT
    GGTGGAGGTG GTGGAGCAAC ATCATTTAGT ATAGGAACAC CGCCCAGCCA TAGTCCGTTC
    CCTGTTTCGG AGTGTGATTA TGATTTGAAG GGTCAAATGG ATTTGGAAAG CGAAGATCCT
    GAGGTGATCC AGATGATTGT TCGTCATCGA ATGGAACAAC ACAAACTTCT CGAAAAGCAA
    CGTGTCGAGA TTGAGCGTCT TCGTTCGAAA ATTCGAGTTC CACGTGCAAC TTCTGTGAAT
    CCCGAAATGA TTGGAGATGA TGAAGCTGAT ACGACGCTTA CTGCTCTTCA AAGTGCATTA
    GGCAATGCTT CATTGTCATT GCCAGCCAGT CCGCCACCTA ATACTGAGAC AACGAAGGTC
    AACACCACTG TAATTCCTTC CGATGTATTG GCGACCCGAA TGACAATGTC TCAATCGTCA
    ACCAAATCAA GCAACGTCTC GGTATCATCC CGTCATCGCG ACAATCAGTC CGCTCCGCCA
    CGTCATCATC ACCATCAACC CCACCCTCCT CATCATCCGC ACCTCCAAAA TCACTATCAT
    CCCCCACAAA ATCATACGTC AGCCACTGCT CCTTGTCCAT CGGCTATGGT TCAACTGCAA
    GCAGTGAGCA ACAACAACGT GAACCCTCTC CATCAGCCAC CACATCCAGT TTCCTCTCAG
    ATCCCGCCAC AGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343983
    Clone ID Related Accession (Same CDS sequence) NM_001268446.1 , NM_001268446.2
    Accession Version NM_001268446.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GGTCAGTGGA GTATCACACT CGGCTTCACC TTCAACGGTA
    GTCGAGTCGC TCACCTCAAT GACTCCACAA ACAATTCCAT TGGCTTGTCA AACTGTGCCC
    GCATCGATCG GTCAAGCTCC AGCAGTCATC GCAGCTGCAC ACGCGGCGTC ATTAATTCCA
    AATGCAAGTG TTCCACAGTC ACCTAGTCGT CTTGATGCCG AAACTGGACT TGCCGGTCTT
    CACGAGAAGC TTGAAGCGCT GAAAATGGAA CAGGATCGAC GAGAAGATAT GGGAGATGAT
    GCGATTGGCA CAACAACCAC AGACGGAAAG GACGAGATAC CGATTGATAC GTTAAAAGGG
    CTCGCGGAAG CATTAGGAAA AGTTATTCAT GCGGATGGTA GAGAAACAAC ACCAATGCCA
    CCGGATCATC CGGATTTAAC AGATGCTTCA ACGCAACAAC TCATCTCACC ATCTAACCCT
    GATGTTTTGA CAACGATGTC GTCGGCTGTC GAAGGATCAG CATCATCCAC AATGATAGAA
    GACATTGATG CTTCAACATC AGCGGTAGAC GCGTCAATGA TGAATTCGAT GCCGCCAGGA
    GCTCAGAACT CTACAGATCA GATACCAGCA GCGATGACAC TGAGTATGGA TCAAGAATGT
    GCTCAATCGA TGACGTCATC GATAACAAGA AACACGACAG GCACCAAATT GGCGACTTTT
    GAGAATCTTG AAACAGCTCT ATCTTCCACA CTCGGAACAC ACATTCGTCA ACCGAATGCA
    CCGTCTTCTC GAGATGAGAC GACGGCTCCG ATGACACCAA GTTTCACAAA TGAAAGAATT
    GGTGGTGGTG GAGGTGGTGG AGCAACATCA TTTAGTATAG GAACACCGCC CAGCCATAGT
    CCGTTCCCTG TTTCGGAGTG TGATTATGAT TTGAAGGGTC AAATGGATTT GGAAAGCGAA
    GATCCTGAGG TGATCCAGAT GATTGTTCGT CATCGAATGG AACAACACAA ACTTCTCGAA
    AAGCAACGTG TCGAGATTGA GCGTCTTCGT TCGAAAATTC GAGTTCCACG TGCAACTTCT
    GTGAATCCCG AAATGATTGG AGATGATGAA GCTGATACGA CGCTTACTGC TCTTCAAAGT
    GCATTAGGCA ATGCTTCATT GTCATTGCCA GCCAGTCCGC CACCTAATAC TGAGACAACG
    AAGGTCAACA CCACTGTAAT TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA
    TCGTCAACCA AATCAAGCAA CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT
    CCGCCACGTC ATCATCACCA TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC
    TATCATCCCC CACAAAATCA TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA
    CTGCAAGCAG TGAGCAACAA CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC
    TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255375.1
    CDS1..5364
    Translation

    Target ORF information:

    RefSeq Version NM_001268446.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GGTCAGTGGA GTATCACACT CGGCTTCACC TTCAACGGTA
    GTCGAGTCGC TCACCTCAAT GACTCCACAA ACAATTCCAT TGGCTTGTCA AACTGTGCCC
    GCATCGATCG GTCAAGCTCC AGCAGTCATC GCAGCTGCAC ACGCGGCGTC ATTAATTCCA
    AATGCAAGTG TTCCACAGTC ACCTAGTCGT CTTGATGCCG AAACTGGACT TGCCGGTCTT
    CACGAGAAGC TTGAAGCGCT GAAAATGGAA CAGGATCGAC GAGAAGATAT GGGAGATGAT
    GCGATTGGCA CAACAACCAC AGACGGAAAG GACGAGATAC CGATTGATAC GTTAAAAGGG
    CTCGCGGAAG CATTAGGAAA AGTTATTCAT GCGGATGGTA GAGAAACAAC ACCAATGCCA
    CCGGATCATC CGGATTTAAC AGATGCTTCA ACGCAACAAC TCATCTCACC ATCTAACCCT
    GATGTTTTGA CAACGATGTC GTCGGCTGTC GAAGGATCAG CATCATCCAC AATGATAGAA
    GACATTGATG CTTCAACATC AGCGGTAGAC GCGTCAATGA TGAATTCGAT GCCGCCAGGA
    GCTCAGAACT CTACAGATCA GATACCAGCA GCGATGACAC TGAGTATGGA TCAAGAATGT
    GCTCAATCGA TGACGTCATC GATAACAAGA AACACGACAG GCACCAAATT GGCGACTTTT
    GAGAATCTTG AAACAGCTCT ATCTTCCACA CTCGGAACAC ACATTCGTCA ACCGAATGCA
    CCGTCTTCTC GAGATGAGAC GACGGCTCCG ATGACACCAA GTTTCACAAA TGAAAGAATT
    GGTGGTGGTG GAGGTGGTGG AGCAACATCA TTTAGTATAG GAACACCGCC CAGCCATAGT
    CCGTTCCCTG TTTCGGAGTG TGATTATGAT TTGAAGGGTC AAATGGATTT GGAAAGCGAA
    GATCCTGAGG TGATCCAGAT GATTGTTCGT CATCGAATGG AACAACACAA ACTTCTCGAA
    AAGCAACGTG TCGAGATTGA GCGTCTTCGT TCGAAAATTC GAGTTCCACG TGCAACTTCT
    GTGAATCCCG AAATGATTGG AGATGATGAA GCTGATACGA CGCTTACTGC TCTTCAAAGT
    GCATTAGGCA ATGCTTCATT GTCATTGCCA GCCAGTCCGC CACCTAATAC TGAGACAACG
    AAGGTCAACA CCACTGTAAT TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA
    TCGTCAACCA AATCAAGCAA CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT
    CCGCCACGTC ATCATCACCA TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC
    TATCATCCCC CACAAAATCA TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA
    CTGCAAGCAG TGAGCAACAA CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC
    TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343983
    Clone ID Related Accession (Same CDS sequence) NM_001268446.1 , NM_001268446.2
    Accession Version NM_001268446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268446.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GGTCAGTGGA GTATCACACT CGGCTTCACC TTCAACGGTA
    GTCGAGTCGC TCACCTCAAT GACTCCACAA ACAATTCCAT TGGCTTGTCA AACTGTGCCC
    GCATCGATCG GTCAAGCTCC AGCAGTCATC GCAGCTGCAC ACGCGGCGTC ATTAATTCCA
    AATGCAAGTG TTCCACAGTC ACCTAGTCGT CTTGATGCCG AAACTGGACT TGCCGGTCTT
    CACGAGAAGC TTGAAGCGCT GAAAATGGAA CAGGATCGAC GAGAAGATAT GGGAGATGAT
    GCGATTGGCA CAACAACCAC AGACGGAAAG GACGAGATAC CGATTGATAC GTTAAAAGGG
    CTCGCGGAAG CATTAGGAAA AGTTATTCAT GCGGATGGTA GAGAAACAAC ACCAATGCCA
    CCGGATCATC CGGATTTAAC AGATGCTTCA ACGCAACAAC TCATCTCACC ATCTAACCCT
    GATGTTTTGA CAACGATGTC GTCGGCTGTC GAAGGATCAG CATCATCCAC AATGATAGAA
    GACATTGATG CTTCAACATC AGCGGTAGAC GCGTCAATGA TGAATTCGAT GCCGCCAGGA
    GCTCAGAACT CTACAGATCA GATACCAGCA GCGATGACAC TGAGTATGGA TCAAGAATGT
    GCTCAATCGA TGACGTCATC GATAACAAGA AACACGACAG GCACCAAATT GGCGACTTTT
    GAGAATCTTG AAACAGCTCT ATCTTCCACA CTCGGAACAC ACATTCGTCA ACCGAATGCA
    CCGTCTTCTC GAGATGAGAC GACGGCTCCG ATGACACCAA GTTTCACAAA TGAAAGAATT
    GGTGGTGGTG GAGGTGGTGG AGCAACATCA TTTAGTATAG GAACACCGCC CAGCCATAGT
    CCGTTCCCTG TTTCGGAGTG TGATTATGAT TTGAAGGGTC AAATGGATTT GGAAAGCGAA
    GATCCTGAGG TGATCCAGAT GATTGTTCGT CATCGAATGG AACAACACAA ACTTCTCGAA
    AAGCAACGTG TCGAGATTGA GCGTCTTCGT TCGAAAATTC GAGTTCCACG TGCAACTTCT
    GTGAATCCCG AAATGATTGG AGATGATGAA GCTGATACGA CGCTTACTGC TCTTCAAAGT
    GCATTAGGCA ATGCTTCATT GTCATTGCCA GCCAGTCCGC CACCTAATAC TGAGACAACG
    AAGGTCAACA CCACTGTAAT TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA
    TCGTCAACCA AATCAAGCAA CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT
    CCGCCACGTC ATCATCACCA TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC
    TATCATCCCC CACAAAATCA TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA
    CTGCAAGCAG TGAGCAACAA CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC
    TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255375.1
    CDS1..5364
    Translation

    Target ORF information:

    RefSeq Version NM_001268446.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GGTCAGTGGA GTATCACACT CGGCTTCACC TTCAACGGTA
    GTCGAGTCGC TCACCTCAAT GACTCCACAA ACAATTCCAT TGGCTTGTCA AACTGTGCCC
    GCATCGATCG GTCAAGCTCC AGCAGTCATC GCAGCTGCAC ACGCGGCGTC ATTAATTCCA
    AATGCAAGTG TTCCACAGTC ACCTAGTCGT CTTGATGCCG AAACTGGACT TGCCGGTCTT
    CACGAGAAGC TTGAAGCGCT GAAAATGGAA CAGGATCGAC GAGAAGATAT GGGAGATGAT
    GCGATTGGCA CAACAACCAC AGACGGAAAG GACGAGATAC CGATTGATAC GTTAAAAGGG
    CTCGCGGAAG CATTAGGAAA AGTTATTCAT GCGGATGGTA GAGAAACAAC ACCAATGCCA
    CCGGATCATC CGGATTTAAC AGATGCTTCA ACGCAACAAC TCATCTCACC ATCTAACCCT
    GATGTTTTGA CAACGATGTC GTCGGCTGTC GAAGGATCAG CATCATCCAC AATGATAGAA
    GACATTGATG CTTCAACATC AGCGGTAGAC GCGTCAATGA TGAATTCGAT GCCGCCAGGA
    GCTCAGAACT CTACAGATCA GATACCAGCA GCGATGACAC TGAGTATGGA TCAAGAATGT
    GCTCAATCGA TGACGTCATC GATAACAAGA AACACGACAG GCACCAAATT GGCGACTTTT
    GAGAATCTTG AAACAGCTCT ATCTTCCACA CTCGGAACAC ACATTCGTCA ACCGAATGCA
    CCGTCTTCTC GAGATGAGAC GACGGCTCCG ATGACACCAA GTTTCACAAA TGAAAGAATT
    GGTGGTGGTG GAGGTGGTGG AGCAACATCA TTTAGTATAG GAACACCGCC CAGCCATAGT
    CCGTTCCCTG TTTCGGAGTG TGATTATGAT TTGAAGGGTC AAATGGATTT GGAAAGCGAA
    GATCCTGAGG TGATCCAGAT GATTGTTCGT CATCGAATGG AACAACACAA ACTTCTCGAA
    AAGCAACGTG TCGAGATTGA GCGTCTTCGT TCGAAAATTC GAGTTCCACG TGCAACTTCT
    GTGAATCCCG AAATGATTGG AGATGATGAA GCTGATACGA CGCTTACTGC TCTTCAAAGT
    GCATTAGGCA ATGCTTCATT GTCATTGCCA GCCAGTCCGC CACCTAATAC TGAGACAACG
    AAGGTCAACA CCACTGTAAT TCCTTCCGAT GTATTGGCGA CCCGAATGAC AATGTCTCAA
    TCGTCAACCA AATCAAGCAA CGTCTCGGTA TCATCCCGTC ATCGCGACAA TCAGTCCGCT
    CCGCCACGTC ATCATCACCA TCAACCCCAC CCTCCTCATC ATCCGCACCT CCAAAATCAC
    TATCATCCCC CACAAAATCA TACGTCAGCC ACTGCTCCTT GTCCATCGGC TATGGTTCAA
    CTGCAAGCAG TGAGCAACAA CAACGTGAAC CCTCTCCATC AGCCACCACA TCCAGTTTCC
    TCTCAGATCC CGCCACAGGC GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343984
    Clone ID Related Accession (Same CDS sequence) NM_001268440.1 , NM_001268440.2
    Accession Version NM_001268440.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA CAACGAAGGT CAACACCACT
    GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA
    AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT
    CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA
    AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC
    AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA
    CAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255369.1
    CDS1..5049
    Translation

    Target ORF information:

    RefSeq Version NM_001268440.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA CAACGAAGGT CAACACCACT
    GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA
    AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT
    CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA
    AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC
    AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA
    CAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343984
    Clone ID Related Accession (Same CDS sequence) NM_001268440.1 , NM_001268440.2
    Accession Version NM_001268440.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268440.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA CAACGAAGGT CAACACCACT
    GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA
    AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT
    CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA
    AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC
    AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA
    CAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255369.1
    CDS316..5364
    Translation

    Target ORF information:

    RefSeq Version NM_001268440.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268440.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GGGTCAAATG GATTTGGAAA GCGAAGATCC TGAGGTGATC
    CAGATGATTG TTCGTCATCG AATGGAACAA CACAAACTTC TCGAAAAGCA ACGTGTCGAG
    ATTGAGCGTC TTCGTTCGAA AATTCGAGTT CCACGTGCAA CTTCTGTGAA TCCCGAAATG
    ATTGGAGATG ATGAAGCTGA TACGACGCTT ACTGCTCTTC AAAGTGCATT AGGCAATGCT
    TCATTGTCAT TGCCAGCCAG TCCGCCACCT AATACTGAGA CAACGAAGGT CAACACCACT
    GTAATTCCTT CCGATGTATT GGCGACCCGA ATGACAATGT CTCAATCGTC AACCAAATCA
    AGCAACGTCT CGGTATCATC CCGTCATCGC GACAATCAGT CCGCTCCGCC ACGTCATCAT
    CACCATCAAC CCCACCCTCC TCATCATCCG CACCTCCAAA ATCACTATCA TCCCCCACAA
    AATCATACGT CAGCCACTGC TCCTTGTCCA TCGGCTATGG TTCAACTGCA AGCAGTGAGC
    AACAACAACG TGAACCCTCT CCATCAGCCA CCACATCCAG TTTCCTCTCA GATCCCGCCA
    CAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343985
    Clone ID Related Accession (Same CDS sequence) NM_001268447.1 , NM_001268447.2
    Accession Version NM_001268447.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGTGTTT TCAGGTCAGT GGAGTATCAC ACTCGGCTTC ACCTTCAACG
    GTAGTCGAGT CGCTCACCTC AATGACTCCA CAAACAATTC CATTGGCTTG TCAAACTGTG
    CCCGCATCGA TCGGTCAAGC TCCAGCAGTC ATCGCAGCTG CACACGCGGC GTCATTAATT
    CCAAATGCAA GTGTTCCACA GTCACCTAGT CGTCTTGATG CCGAAACTGG ACTTGCCGGT
    CTTCACGAGA AGCTTGAAGC GCTGAAAATG GAACAGGATC GACGAGAAGA TATGGGAGAT
    GATGCGATTG GCACAACAAC CACAGACGGA AAGGACGAGA TACCGATTGA TACGTTAAAA
    GGGCTCGCGG AAGCATTAGG AAAAGTTATT CATGCGGATG GTAGAGAAAC AACACCAATG
    CCACCGGATC ATCCGGATTT AACAGATGCT TCAACGCAAC AACTCATCTC ACCATCTAAC
    CCTGATGTTT TGACAACGAT GTCGTCGGCT GTCGAAGGAT CAGCATCATC CACAATGATA
    GAAGACATTG ATGCTTCAAC ATCAGCGGTA GACGCGTCAA TGATGAATTC GATGCCGCCA
    GGAGCTCAGA ACTCTACAGA TCAGATACCA GCAGCGATGA CACTGAGTAT GGATCAAGAA
    TGTGCTCAAT CGATGACGTC ATCGATAACA AGAAACACGA CAGGCACCAA ATTGGCGACT
    TTTGAGAATC TTGAAACAGC TCTATCTTCC ACACTCGGAA CACACATTCG TCAACCGAAT
    GCACCGTCTT CTCGAGATGA GACGACGGCT CCGATGACAC CAAGTTTCAC AAATGAAAGA
    ATTGGTGGTG GTGGAGGTGG TGGAGCAACA TCATTTAGTA TAGGAACACC GCCCAGCCAT
    AGTCCGTTCC CTGTTTCGGA GTGTGATTAT GATTTGAAGG GTCAAATGGA TTTGGAAAGC
    GAAGATCCTG AGGTGATCCA GATGATTGTT CGTCATCGAA TGGAACAACA CAAACTTCTC
    GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT CGTTCGAAAA TTCGAGTTCC ACGTGCAACT
    TCTGTGAATC CCGAAATGAT TGGAGATGAT GAAGCTGATA CGACGCTTAC TGCTCTTCAA
    AGTGCATTAG GCAATGCTTC ATTGTCATTG CCAGCCAGTC CGCCACCTAA TACTGAGACA
    ACGAAGGTCA ACACCACTGT AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT
    CAATCGTCAA CCAAATCAAG CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC
    GCTCCGCCAC GTCATCATCA CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT
    CACTATCATC CCCCACAAAA TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT
    CAACTGCAAG CAGTGAGCAA CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT
    TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255376.1
    CDS1..5367
    Translation

    Target ORF information:

    RefSeq Version NM_001268447.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGTGTTT TCAGGTCAGT GGAGTATCAC ACTCGGCTTC ACCTTCAACG
    GTAGTCGAGT CGCTCACCTC AATGACTCCA CAAACAATTC CATTGGCTTG TCAAACTGTG
    CCCGCATCGA TCGGTCAAGC TCCAGCAGTC ATCGCAGCTG CACACGCGGC GTCATTAATT
    CCAAATGCAA GTGTTCCACA GTCACCTAGT CGTCTTGATG CCGAAACTGG ACTTGCCGGT
    CTTCACGAGA AGCTTGAAGC GCTGAAAATG GAACAGGATC GACGAGAAGA TATGGGAGAT
    GATGCGATTG GCACAACAAC CACAGACGGA AAGGACGAGA TACCGATTGA TACGTTAAAA
    GGGCTCGCGG AAGCATTAGG AAAAGTTATT CATGCGGATG GTAGAGAAAC AACACCAATG
    CCACCGGATC ATCCGGATTT AACAGATGCT TCAACGCAAC AACTCATCTC ACCATCTAAC
    CCTGATGTTT TGACAACGAT GTCGTCGGCT GTCGAAGGAT CAGCATCATC CACAATGATA
    GAAGACATTG ATGCTTCAAC ATCAGCGGTA GACGCGTCAA TGATGAATTC GATGCCGCCA
    GGAGCTCAGA ACTCTACAGA TCAGATACCA GCAGCGATGA CACTGAGTAT GGATCAAGAA
    TGTGCTCAAT CGATGACGTC ATCGATAACA AGAAACACGA CAGGCACCAA ATTGGCGACT
    TTTGAGAATC TTGAAACAGC TCTATCTTCC ACACTCGGAA CACACATTCG TCAACCGAAT
    GCACCGTCTT CTCGAGATGA GACGACGGCT CCGATGACAC CAAGTTTCAC AAATGAAAGA
    ATTGGTGGTG GTGGAGGTGG TGGAGCAACA TCATTTAGTA TAGGAACACC GCCCAGCCAT
    AGTCCGTTCC CTGTTTCGGA GTGTGATTAT GATTTGAAGG GTCAAATGGA TTTGGAAAGC
    GAAGATCCTG AGGTGATCCA GATGATTGTT CGTCATCGAA TGGAACAACA CAAACTTCTC
    GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT CGTTCGAAAA TTCGAGTTCC ACGTGCAACT
    TCTGTGAATC CCGAAATGAT TGGAGATGAT GAAGCTGATA CGACGCTTAC TGCTCTTCAA
    AGTGCATTAG GCAATGCTTC ATTGTCATTG CCAGCCAGTC CGCCACCTAA TACTGAGACA
    ACGAAGGTCA ACACCACTGT AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT
    CAATCGTCAA CCAAATCAAG CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC
    GCTCCGCCAC GTCATCATCA CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT
    CACTATCATC CCCCACAAAA TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT
    CAACTGCAAG CAGTGAGCAA CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT
    TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343985
    Clone ID Related Accession (Same CDS sequence) NM_001268447.1 , NM_001268447.2
    Accession Version NM_001268447.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268447.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGTGTTT TCAGGTCAGT GGAGTATCAC ACTCGGCTTC ACCTTCAACG
    GTAGTCGAGT CGCTCACCTC AATGACTCCA CAAACAATTC CATTGGCTTG TCAAACTGTG
    CCCGCATCGA TCGGTCAAGC TCCAGCAGTC ATCGCAGCTG CACACGCGGC GTCATTAATT
    CCAAATGCAA GTGTTCCACA GTCACCTAGT CGTCTTGATG CCGAAACTGG ACTTGCCGGT
    CTTCACGAGA AGCTTGAAGC GCTGAAAATG GAACAGGATC GACGAGAAGA TATGGGAGAT
    GATGCGATTG GCACAACAAC CACAGACGGA AAGGACGAGA TACCGATTGA TACGTTAAAA
    GGGCTCGCGG AAGCATTAGG AAAAGTTATT CATGCGGATG GTAGAGAAAC AACACCAATG
    CCACCGGATC ATCCGGATTT AACAGATGCT TCAACGCAAC AACTCATCTC ACCATCTAAC
    CCTGATGTTT TGACAACGAT GTCGTCGGCT GTCGAAGGAT CAGCATCATC CACAATGATA
    GAAGACATTG ATGCTTCAAC ATCAGCGGTA GACGCGTCAA TGATGAATTC GATGCCGCCA
    GGAGCTCAGA ACTCTACAGA TCAGATACCA GCAGCGATGA CACTGAGTAT GGATCAAGAA
    TGTGCTCAAT CGATGACGTC ATCGATAACA AGAAACACGA CAGGCACCAA ATTGGCGACT
    TTTGAGAATC TTGAAACAGC TCTATCTTCC ACACTCGGAA CACACATTCG TCAACCGAAT
    GCACCGTCTT CTCGAGATGA GACGACGGCT CCGATGACAC CAAGTTTCAC AAATGAAAGA
    ATTGGTGGTG GTGGAGGTGG TGGAGCAACA TCATTTAGTA TAGGAACACC GCCCAGCCAT
    AGTCCGTTCC CTGTTTCGGA GTGTGATTAT GATTTGAAGG GTCAAATGGA TTTGGAAAGC
    GAAGATCCTG AGGTGATCCA GATGATTGTT CGTCATCGAA TGGAACAACA CAAACTTCTC
    GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT CGTTCGAAAA TTCGAGTTCC ACGTGCAACT
    TCTGTGAATC CCGAAATGAT TGGAGATGAT GAAGCTGATA CGACGCTTAC TGCTCTTCAA
    AGTGCATTAG GCAATGCTTC ATTGTCATTG CCAGCCAGTC CGCCACCTAA TACTGAGACA
    ACGAAGGTCA ACACCACTGT AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT
    CAATCGTCAA CCAAATCAAG CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC
    GCTCCGCCAC GTCATCATCA CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT
    CACTATCATC CCCCACAAAA TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT
    CAACTGCAAG CAGTGAGCAA CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT
    TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255376.1
    CDS1..5367
    Translation

    Target ORF information:

    RefSeq Version NM_001268447.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATGGACT TCAAAAAAGA GACCAGATGA TCCAACGACA
    TCATCTCCAT CGACAGTTTC AATTAGTAAT GCATTGGAAA ATTCAACACC TTCATTAAAC
    AATGTTTCTT CGATCACAAA CTCTTCATCA CCATTTTCTT TATCGTCAGC TGCAACATCG
    ACAGCTTCTG CCATAATCCC ATTTACCTCA AATGTAGCTA CAAATCATCC ACATCTTAAT
    CACCACGTGT CGAGAATACC ACAGGCGATT GTAACAGGAG GGACAAATGG TTCATTACCT
    CCATTATTAA TATCACCGAC GTCAGCAGCA GCCGCAACGC CGTTGATTTC AGGAAAAGCG
    GGTCCAATGT CACCATCAAC GGGAAGTCCA ATTAATGTGG CTGCTACAGT ATTACAGAAT
    GCAGTTTCAA GTCCACAACA TAGTATTTTT GATAGATCTA GGTTAAATAA AATTCCACCA
    AACACATCAT TAGCATCGTC TTCGTCTCCG AGTGATGCTG CTAACAATGA TAAACCTATA
    CAACAACGTC ATAGTATTTT ATCGAATGTT CGAACTCTCA CTCAAGCAAT GGTAAACGAT
    GGACCACGAA CGTTGACTGG AGATGATATG GACAAGATGG TCAGTGAAGA GGAAAGAGCA
    CGAAAAGAGC AAGAGAAGCG AGAAGAAGAA GAGAAAGCAG CACGGAGAAT TGATGTTGAA
    GATGATTTTG ATGCGCAAGA AAAACCCATT GATAAGAGTA AAAATGGTCG ATTCTTAAAG
    TTCGACGAAG AACTTGGTCG CGGATCGTTC AAGACAGTGT TTCGAGGGTT GGACACTGAA
    ACCGGAGTGG CCGTTGCGTG GTGTGAGCTT CAGGAGAGTA AACTGAATAA GACTGAAAGG
    CAACGTTTTC GAGAAGAAGC TGAAATGCTC AAGGATCTGC AACATCCGAA CATCGTACGA
    TTCTACGATT ATTGGGAGAG TGCGGATTTG TGTGGAAAAC GGAAATATAT TGTGCTTGTA
    ACCGAATTGA TGACTTCAGG AACATTAAAA ATGTATTTGA AGCGATTTAA ACGGATAAAC
    ATAAAGGTAT TGAAATCTTG GTGCAGACAA ATTCTGAAAG GGCTTTCATT TCTTCACACA
    CGTAATCCAC CTGTAATTCA TCGTGATCTC AAGTGTGATA ATATCTTTAT CACTGGTACA
    ACTGGTAGTG TAAAGATTGG AGATCTTGGA TTGGCAACTC TTAAAAATAA ATCATTCGCG
    AAATCTGTCA TTGGAACTCC AGAATTCATG GCACCGGAAA TGTACGAAGA GATGTATGAT
    GAGAGTGTAG ATGTCTACGC GTTTGGAATG TGTCTTCTTG AAATGGTTAC CGGAGAATAT
    CCATATTCTG AGTGTATGAA TCCAGCGACA ATCTACAGAA AAGTGATATC AGGTGTCAAA
    CCAGAATGTT TCTCAAGAAT TCCTGCACAA TATCCTGAAA TACGTGAGAT AATCGATCGG
    TGTATTCGTG TGAGACGAGA AGAGAGAAGT ACCGTGAAGC AATTGCTCGT TGACGATTTC
    TTCACTCCAG AAGATCTCAT CGGAATTCGT GTTGAAATTA AGAATCGGGA TGCTGATCTC
    AATGATCTTA ACGTGGAAAT TCAAATGCAA CTCCGAGTTT ACGATGAGAA AAAGAGAAAA
    CAATATCGGT TCAAGGAGAA CGAAGGACTT CAATTTGCAT TTGATATTGA AAATGACAGC
    CCAGATGAAG TTGTTCAACA GATGATCGAG CAACAACATA TTCCAGATGA GGATACTCGA
    ATGATAACAA AGTTGATAAA AGACAAAGTG GATGCGTTCA GAAGAGATCG AGATCATCGT
    TTATTGGAGA TTAAAAGAGC AAAAGAGGAG GAAGAAAGGA TACGAGAAGA GGCTGAGATT
    AAAGAGGAAT TGAGGTTGAG AGCTGAAGCT AAAGAAAAGG AAAAGGAGAG GTTGGAGAAA
    GAACGATTAG AAAAAAAAGC TGCAGCTGCG GCCGCAGCCA ATCCGAATCC TACACCAATT
    CCACCAACTC CAGCAACACC ACACTCTTCT GCCCAACAAC AGCCTATTCC ACCACCACTA
    TCAACTCAAA CTTCGGCTGA AATACAGCAG TCTGCTCAAC AGCCATCTGT ACCTGTAACT
    ATGATCGCAA ATATCCCAGC TATGTCGCCA ACATCAGCTC AACCACAACC TGTTTTATCA
    CCAACAAGTG CAGCGGTTCC AGTTCCAACG ACAATGATTC ATGTTCCAAA ACCTAGCGAG
    ATTCCAGTGC AGAATGTAGC GACGACTGCA GCCCCGGTTG CGGCTAACAA TGTACCACCA
    TCACCAGCTC CATTCAAAAC AGAAGATATC CAAACTCCCA CACTTGCCCA GAATACGGTT
    CCACGAACAA TCTCCACTGA TGCTTCTGGA CTTGTCATCA ATACTCCTGC ATCAATAGCA
    TCACCATCAC CTGCTCCTTC GGCTACTGAT GTTGCTTCAA CAACGGCTCC AGTCACCCCT
    GCTCCAACTC CAACAACAAC AACGGACGGT GGTGCAGCAG CAGCTTCAAC AACAACTGAA
    AACAAGGAAG AAAAGCGAAA AAGCAACAAA AGAAAAGTGG TAATGGAGAT TTTGGGATGT
    GATGAATCGA GGAATTTTGC ATTGGTCTCG TGTCGACTTG ACACTTCTCA TAAATCTGTT
    ACCTTCCAAT TTGCACCAGG AACTGACAAA CCATGCACAA TTGCAACGAA ACTTTTGGCT
    GAAGATTGTC TTTTAAAAGT TCATGTTCAT ATTGTTGAAG CACAATTGGG AGAAGTTATT
    CAATTGATTA ATTCTGATGG AAAGAAAGGT GTTGGTACTA AATTAGCGAC GGTATTGGAT
    CCAAATAGTA CTGAACCACC AACTATCACT GCTGTCATGC CAAAGGATTC CTCTGCCGCA
    ACTGCTTCTA ATACTAAGCC TAAGATTGAA ATCGAGAAAA CGCCACCAAC GAGAGATGCT
    TCCCAAGAGC CGAATAATGT TCAGGTTACG AACGTACGCA AAGTTTCACA AGAATCAAAT
    GCCGAAAGTG TTCAATCAAT TCCTCGTCCA GGTGGAATCA TTGTGATGTC CCCAACAAAT
    CAAACAGATT CGGCTCCGCC CCCAACTGGA GCTGCAGCAA AACCATCACG ATTCCAAGTA
    ACAAAGTCAG CGGATCCTAT AGCTACACCG ATATCTTCGT CAATATCCAC AGCAACAGTT
    ATCCCAATTG TTGCAGCAAC ACCTACGAAT ATCACATCCG AACCGGTCAT TGTCCAACCA
    ATCACTGCAC AAGTTATAAC CCATTTGGCT ACACCATCGC CAGTTTCTCA TTCGTTATCA
    TCAAATTCTT CTCCAAGTGC TACAACTCAT TCGAATATGT CATCAATTCA ATCAACGACT
    AGCGTTCCTG GAAGACGATT CACAGTTCAG CCCGTCTCTC AAGCAGAATC CGGAATATCT
    TCGTCAATTT CTACGCCTCA TCCGGAACCG ACGCCAGCAA TCACTTCGTG TCCTCCACCA
    GTTCCAAGTG TCCCACCAGT GGTGTCCAAT GGGACATTGA ACTTAGAAGT TGCTCCTAAA
    CAGACTCCAT CAGCAACTAA CCAAAATGTC GATACACAAC ATTCATCGTC AACAGCATCA
    ACAGCTACAT TAGTTTCGGA GACACCTGCT ACAGTACATG TTACTCCGAT CTCAGTGCCA
    GCTCCTGTTC AAGAACCATT AGTCATCGAT CATCATTCTG ATGTATTGAC ACAATTGGAT
    AGTGAGTTGA GAAAGTGTTT TCAGGTCAGT GGAGTATCAC ACTCGGCTTC ACCTTCAACG
    GTAGTCGAGT CGCTCACCTC AATGACTCCA CAAACAATTC CATTGGCTTG TCAAACTGTG
    CCCGCATCGA TCGGTCAAGC TCCAGCAGTC ATCGCAGCTG CACACGCGGC GTCATTAATT
    CCAAATGCAA GTGTTCCACA GTCACCTAGT CGTCTTGATG CCGAAACTGG ACTTGCCGGT
    CTTCACGAGA AGCTTGAAGC GCTGAAAATG GAACAGGATC GACGAGAAGA TATGGGAGAT
    GATGCGATTG GCACAACAAC CACAGACGGA AAGGACGAGA TACCGATTGA TACGTTAAAA
    GGGCTCGCGG AAGCATTAGG AAAAGTTATT CATGCGGATG GTAGAGAAAC AACACCAATG
    CCACCGGATC ATCCGGATTT AACAGATGCT TCAACGCAAC AACTCATCTC ACCATCTAAC
    CCTGATGTTT TGACAACGAT GTCGTCGGCT GTCGAAGGAT CAGCATCATC CACAATGATA
    GAAGACATTG ATGCTTCAAC ATCAGCGGTA GACGCGTCAA TGATGAATTC GATGCCGCCA
    GGAGCTCAGA ACTCTACAGA TCAGATACCA GCAGCGATGA CACTGAGTAT GGATCAAGAA
    TGTGCTCAAT CGATGACGTC ATCGATAACA AGAAACACGA CAGGCACCAA ATTGGCGACT
    TTTGAGAATC TTGAAACAGC TCTATCTTCC ACACTCGGAA CACACATTCG TCAACCGAAT
    GCACCGTCTT CTCGAGATGA GACGACGGCT CCGATGACAC CAAGTTTCAC AAATGAAAGA
    ATTGGTGGTG GTGGAGGTGG TGGAGCAACA TCATTTAGTA TAGGAACACC GCCCAGCCAT
    AGTCCGTTCC CTGTTTCGGA GTGTGATTAT GATTTGAAGG GTCAAATGGA TTTGGAAAGC
    GAAGATCCTG AGGTGATCCA GATGATTGTT CGTCATCGAA TGGAACAACA CAAACTTCTC
    GAAAAGCAAC GTGTCGAGAT TGAGCGTCTT CGTTCGAAAA TTCGAGTTCC ACGTGCAACT
    TCTGTGAATC CCGAAATGAT TGGAGATGAT GAAGCTGATA CGACGCTTAC TGCTCTTCAA
    AGTGCATTAG GCAATGCTTC ATTGTCATTG CCAGCCAGTC CGCCACCTAA TACTGAGACA
    ACGAAGGTCA ACACCACTGT AATTCCTTCC GATGTATTGG CGACCCGAAT GACAATGTCT
    CAATCGTCAA CCAAATCAAG CAACGTCTCG GTATCATCCC GTCATCGCGA CAATCAGTCC
    GCTCCGCCAC GTCATCATCA CCATCAACCC CACCCTCCTC ATCATCCGCA CCTCCAAAAT
    CACTATCATC CCCCACAAAA TCATACGTCA GCCACTGCTC CTTGTCCATC GGCTATGGTT
    CAACTGCAAG CAGTGAGCAA CAACAACGTG AACCCTCTCC ATCAGCCACC ACATCCAGTT
    TCCTCTCAGA TCCCGCCACA GGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343986
    Clone ID Related Accession (Same CDS sequence) NM_001268445.1 , NM_001268445.2
    Accession Version NM_001268445.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GTGTTTTCAG GTCAGTGGAG TATCACACTC GGCTTCACCT
    TCAACGGTAG TCGAGTCGCT CACCTCAATG ACTCCACAAA CAATTCCATT GGCTTGTCAA
    ACTGTGCCCG CATCGATCGG TCAAGCTCCA GCAGTCATCG CAGCTGCACA CGCGGCGTCA
    TTAATTCCAA ATGCAAGTGT TCCACAGTCA CCTAGTCGTC TTGATGCCGA AACTGGACTT
    GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG AAAATGGAAC AGGATCGACG AGAAGATATG
    GGAGATGATG CGATTGGCAC AACAACCACA GACGGAAAGG ACGAGATACC GATTGATACG
    TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA GTTATTCATG CGGATGGTAG AGAAACAACA
    CCAATGCCAC CGGATCATCC GGATTTAACA GATGCTTCAA CGCAACAACT CATCTCACCA
    TCTAACCCTG ATGTTTTGAC AACGATGTCG TCGGCTGTCG AAGGATCAGC ATCATCCACA
    ATGATAGAAG ACATTGATGC TTCAACATCA GCGGTAGACG CGTCAATGAT GAATTCGATG
    CCGCCAGGAG CTCAGAACTC TACAGATCAG ATACCAGCAG CGATGACACT GAGTATGGAT
    CAAGAATGTG CTCAATCGAT GACGTCATCG ATAACAAGAA ACACGACAGG CACCAAATTG
    GCGACTTTTG AGAATCTTGA AACAGCTCTA TCTTCCACAC TCGGAACACA CATTCGTCAA
    CCGAATGCAC CGTCTTCTCG AGATGAGACG ACGGCTCCGA TGACACCAAG TTTCACAAAT
    GAAAGAATTG GTGGTGGTGG AGGTGGTGGA GCAACATCAT TTAGTATAGG AACACCGCCC
    AGCCATAGTC CGTTCCCTGT TTCGGAGTGT GATTATGATT TGAAGGGTCA AATGGATTTG
    GAAAGCGAAG ATCCTGAGGT GATCCAGATG ATTGTTCGTC ATCGAATGGA ACAACACAAA
    CTTCTCGAAA AGCAACGTGT CGAGATTGAG CGTCTTCGTT CGAAAATTCG AGTTCCACGT
    GCAACTTCTG TGAATCCCGA AATGATTGGA GATGATGAAG CTGATACGAC GCTTACTGCT
    CTTCAAAGTG CATTAGGCAA TGCTTCATTG TCATTGCCAG CCAGTCCGCC ACCTAATACT
    GAGACAACGA AGGTCAACAC CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA
    ATGTCTCAAT CGTCAACCAA ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT
    CAGTCCGCTC CGCCACGTCA TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC
    CAAAATCACT ATCATCCCCC ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT
    ATGGTTCAAC TGCAAGCAGT GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT
    CCAGTTTCCT CTCAGATCCC GCCACAGGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255374.1
    CDS1..5373
    Translation

    Target ORF information:

    RefSeq Version NM_001268445.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GTGTTTTCAG GTCAGTGGAG TATCACACTC GGCTTCACCT
    TCAACGGTAG TCGAGTCGCT CACCTCAATG ACTCCACAAA CAATTCCATT GGCTTGTCAA
    ACTGTGCCCG CATCGATCGG TCAAGCTCCA GCAGTCATCG CAGCTGCACA CGCGGCGTCA
    TTAATTCCAA ATGCAAGTGT TCCACAGTCA CCTAGTCGTC TTGATGCCGA AACTGGACTT
    GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG AAAATGGAAC AGGATCGACG AGAAGATATG
    GGAGATGATG CGATTGGCAC AACAACCACA GACGGAAAGG ACGAGATACC GATTGATACG
    TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA GTTATTCATG CGGATGGTAG AGAAACAACA
    CCAATGCCAC CGGATCATCC GGATTTAACA GATGCTTCAA CGCAACAACT CATCTCACCA
    TCTAACCCTG ATGTTTTGAC AACGATGTCG TCGGCTGTCG AAGGATCAGC ATCATCCACA
    ATGATAGAAG ACATTGATGC TTCAACATCA GCGGTAGACG CGTCAATGAT GAATTCGATG
    CCGCCAGGAG CTCAGAACTC TACAGATCAG ATACCAGCAG CGATGACACT GAGTATGGAT
    CAAGAATGTG CTCAATCGAT GACGTCATCG ATAACAAGAA ACACGACAGG CACCAAATTG
    GCGACTTTTG AGAATCTTGA AACAGCTCTA TCTTCCACAC TCGGAACACA CATTCGTCAA
    CCGAATGCAC CGTCTTCTCG AGATGAGACG ACGGCTCCGA TGACACCAAG TTTCACAAAT
    GAAAGAATTG GTGGTGGTGG AGGTGGTGGA GCAACATCAT TTAGTATAGG AACACCGCCC
    AGCCATAGTC CGTTCCCTGT TTCGGAGTGT GATTATGATT TGAAGGGTCA AATGGATTTG
    GAAAGCGAAG ATCCTGAGGT GATCCAGATG ATTGTTCGTC ATCGAATGGA ACAACACAAA
    CTTCTCGAAA AGCAACGTGT CGAGATTGAG CGTCTTCGTT CGAAAATTCG AGTTCCACGT
    GCAACTTCTG TGAATCCCGA AATGATTGGA GATGATGAAG CTGATACGAC GCTTACTGCT
    CTTCAAAGTG CATTAGGCAA TGCTTCATTG TCATTGCCAG CCAGTCCGCC ACCTAATACT
    GAGACAACGA AGGTCAACAC CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA
    ATGTCTCAAT CGTCAACCAA ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT
    CAGTCCGCTC CGCCACGTCA TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC
    CAAAATCACT ATCATCCCCC ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT
    ATGGTTCAAC TGCAAGCAGT GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT
    CCAGTTTCCT CTCAGATCCC GCCACAGGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343986
    Clone ID Related Accession (Same CDS sequence) NM_001268445.1 , NM_001268445.2
    Accession Version NM_001268445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268445.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GTGTTTTCAG GTCAGTGGAG TATCACACTC GGCTTCACCT
    TCAACGGTAG TCGAGTCGCT CACCTCAATG ACTCCACAAA CAATTCCATT GGCTTGTCAA
    ACTGTGCCCG CATCGATCGG TCAAGCTCCA GCAGTCATCG CAGCTGCACA CGCGGCGTCA
    TTAATTCCAA ATGCAAGTGT TCCACAGTCA CCTAGTCGTC TTGATGCCGA AACTGGACTT
    GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG AAAATGGAAC AGGATCGACG AGAAGATATG
    GGAGATGATG CGATTGGCAC AACAACCACA GACGGAAAGG ACGAGATACC GATTGATACG
    TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA GTTATTCATG CGGATGGTAG AGAAACAACA
    CCAATGCCAC CGGATCATCC GGATTTAACA GATGCTTCAA CGCAACAACT CATCTCACCA
    TCTAACCCTG ATGTTTTGAC AACGATGTCG TCGGCTGTCG AAGGATCAGC ATCATCCACA
    ATGATAGAAG ACATTGATGC TTCAACATCA GCGGTAGACG CGTCAATGAT GAATTCGATG
    CCGCCAGGAG CTCAGAACTC TACAGATCAG ATACCAGCAG CGATGACACT GAGTATGGAT
    CAAGAATGTG CTCAATCGAT GACGTCATCG ATAACAAGAA ACACGACAGG CACCAAATTG
    GCGACTTTTG AGAATCTTGA AACAGCTCTA TCTTCCACAC TCGGAACACA CATTCGTCAA
    CCGAATGCAC CGTCTTCTCG AGATGAGACG ACGGCTCCGA TGACACCAAG TTTCACAAAT
    GAAAGAATTG GTGGTGGTGG AGGTGGTGGA GCAACATCAT TTAGTATAGG AACACCGCCC
    AGCCATAGTC CGTTCCCTGT TTCGGAGTGT GATTATGATT TGAAGGGTCA AATGGATTTG
    GAAAGCGAAG ATCCTGAGGT GATCCAGATG ATTGTTCGTC ATCGAATGGA ACAACACAAA
    CTTCTCGAAA AGCAACGTGT CGAGATTGAG CGTCTTCGTT CGAAAATTCG AGTTCCACGT
    GCAACTTCTG TGAATCCCGA AATGATTGGA GATGATGAAG CTGATACGAC GCTTACTGCT
    CTTCAAAGTG CATTAGGCAA TGCTTCATTG TCATTGCCAG CCAGTCCGCC ACCTAATACT
    GAGACAACGA AGGTCAACAC CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA
    ATGTCTCAAT CGTCAACCAA ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT
    CAGTCCGCTC CGCCACGTCA TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC
    CAAAATCACT ATCATCCCCC ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT
    ATGGTTCAAC TGCAAGCAGT GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT
    CCAGTTTCCT CTCAGATCCC GCCACAGGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255374.1
    CDS1..5373
    Translation

    Target ORF information:

    RefSeq Version NM_001268445.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGGATCAC ACTGGTTGTC CGAGCAAGAA AGATCACGAC TTGAAGCTGT TCAACAGGAT 
    TGGCGAAGAA CTCGACCAAT GAAATTTCAG TGGACTTCAA AAAAGAGACC AGATGATCCA
    ACGACATCAT CTCCATCGAC AGTTTCAATT AGTAATGCAT TGGAAAATTC AACACCTTCA
    TTAAACAATG TTTCTTCGAT CACAAACTCT TCATCACCAT TTTCTTTATC GTCAGCTGCA
    ACATCGACAG CTTCTGCCAT AATCCCATTT ACCTCAAATG TAGCTACAAA TCATCCACAT
    CTTAATCACC ACGTGTCGAG AATACCACAG GCGATTGTAA CAGGAGGGAC AAATGGTTCA
    TTACCTCCAT TATTAATATC ACCGACGTCA GCAGCAGCCG CAACGCCGTT GATTTCAGGA
    AAAGCGGGTC CAATGTCACC ATCAACGGGA AGTCCAATTA ATGTGGCTGC TACAGTATTA
    CAGAATGCAG TTTCAAGTCC ACAACATAGT ATTTTTGATA GATCTAGGTT AAATAAAATT
    CCACCAAACA CATCATTAGC ATCGTCTTCG TCTCCGAGTG ATGCTGCTAA CAATGATAAA
    CCTATACAAC AACGTCATAG TATTTTATCG AATGTTCGAA CTCTCACTCA AGCAATGGTA
    AACGATGGAC CACGAACGTT GACTGGAGAT GATATGGACA AGATGGTCAG TGAAGAGGAA
    AGAGCACGAA AAGAGCAAGA GAAGCGAGAA GAAGAAGAGA AAGCAGCACG GAGAATTGAT
    GTTGAAGATG ATTTTGATGC GCAAGAAAAA CCCATTGATA AGAGTAAAAA TGGTCGATTC
    TTAAAGTTCG ACGAAGAACT TGGTCGCGGA TCGTTCAAGA CAGTGTTTCG AGGGTTGGAC
    ACTGAAACCG GAGTGGCCGT TGCGTGGTGT GAGCTTCAGG AGAGTAAACT GAATAAGACT
    GAAAGGCAAC GTTTTCGAGA AGAAGCTGAA ATGCTCAAGG ATCTGCAACA TCCGAACATC
    GTACGATTCT ACGATTATTG GGAGAGTGCG GATTTGTGTG GAAAACGGAA ATATATTGTG
    CTTGTAACCG AATTGATGAC TTCAGGAACA TTAAAAATGT ATTTGAAGCG ATTTAAACGG
    ATAAACATAA AGGTATTGAA ATCTTGGTGC AGACAAATTC TGAAAGGGCT TTCATTTCTT
    CACACACGTA ATCCACCTGT AATTCATCGT GATCTCAAGT GTGATAATAT CTTTATCACT
    GGTACAACTG GTAGTGTAAA GATTGGAGAT CTTGGATTGG CAACTCTTAA AAATAAATCA
    TTCGCGAAAT CTGTCATTGG AACTCCAGAA TTCATGGCAC CGGAAATGTA CGAAGAGATG
    TATGATGAGA GTGTAGATGT CTACGCGTTT GGAATGTGTC TTCTTGAAAT GGTTACCGGA
    GAATATCCAT ATTCTGAGTG TATGAATCCA GCGACAATCT ACAGAAAAGT GATATCAGGT
    GTCAAACCAG AATGTTTCTC AAGAATTCCT GCACAATATC CTGAAATACG TGAGATAATC
    GATCGGTGTA TTCGTGTGAG ACGAGAAGAG AGAAGTACCG TGAAGCAATT GCTCGTTGAC
    GATTTCTTCA CTCCAGAAGA TCTCATCGGA ATTCGTGTTG AAATTAAGAA TCGGGATGCT
    GATCTCAATG ATCTTAACGT GGAAATTCAA ATGCAACTCC GAGTTTACGA TGAGAAAAAG
    AGAAAACAAT ATCGGTTCAA GGAGAACGAA GGACTTCAAT TTGCATTTGA TATTGAAAAT
    GACAGCCCAG ATGAAGTTGT TCAACAGATG ATCGAGCAAC AACATATTCC AGATGAGGAT
    ACTCGAATGA TAACAAAGTT GATAAAAGAC AAAGTGGATG CGTTCAGAAG AGATCGAGAT
    CATCGTTTAT TGGAGATTAA AAGAGCAAAA GAGGAGGAAG AAAGGATACG AGAAGAGGCT
    GAGATTAAAG AGGAATTGAG GTTGAGAGCT GAAGCTAAAG AAAAGGAAAA GGAGAGGTTG
    GAGAAAGAAC GATTAGAAAA AAAAGCTGCA GCTGCGGCCG CAGCCAATCC GAATCCTACA
    CCAATTCCAC CAACTCCAGC AACACCACAC TCTTCTGCCC AACAACAGCC TATTCCACCA
    CCACTATCAA CTCAAACTTC GGCTGAAATA CAGCAGTCTG CTCAACAGCC ATCTGTACCT
    GTAACTATGA TCGCAAATAT CCCAGCTATG TCGCCAACAT CAGCTCAACC ACAACCTGTT
    TTATCACCAA CAAGTGCAGC GGTTCCAGTT CCAACGACAA TGATTCATGT TCCAAAACCT
    AGCGAGATTC CAGTGCAGAA TGTAGCGACG ACTGCAGCCC CGGTTGCGGC TAACAATGTA
    CCACCATCAC CAGCTCCATT CAAAACAGAA GATATCCAAA CTCCCACACT TGCCCAGAAT
    ACGGTTCCAC GAACAATCTC CACTGATGCT TCTGGACTTG TCATCAATAC TCCTGCATCA
    ATAGCATCAC CATCACCTGC TCCTTCGGCT ACTGATGTTG CTTCAACAAC GGCTCCAGTC
    ACCCCTGCTC CAACTCCAAC AACAACAACG GACGGTGGTG CAGCAGCAGC TTCAACAACA
    ACTGAAAACA AGGAAGAAAA GCGAAAAAGC AACAAAAGAA AAGTGGTAAT GGAGATTTTG
    GGATGTGATG AATCGAGGAA TTTTGCATTG GTCTCGTGTC GACTTGACAC TTCTCATAAA
    TCTGTTACCT TCCAATTTGC ACCAGGAACT GACAAACCAT GCACAATTGC AACGAAACTT
    TTGGCTGAAG ATTGTCTTTT AAAAGTTCAT GTTCATATTG TTGAAGCACA ATTGGGAGAA
    GTTATTCAAT TGATTAATTC TGATGGAAAG AAAGGTGTTG GTACTAAATT AGCGACGGTA
    TTGGATCCAA ATAGTACTGA ACCACCAACT ATCACTGCTG TCATGCCAAA GGATTCCTCT
    GCCGCAACTG CTTCTAATAC TAAGCCTAAG ATTGAAATCG AGAAAACGCC ACCAACGAGA
    GATGCTTCCC AAGAGCCGAA TAATGTTCAG GTTACGAACG TACGCAAAGT TTCACAAGAA
    TCAAATGCCG AAAGTGTTCA ATCAATTCCT CGTCCAGGTG GAATCATTGT GATGTCCCCA
    ACAAATCAAA CAGATTCGGC TCCGCCCCCA ACTGGAGCTG CAGCAAAACC ATCACGATTC
    CAAGTAACAA AGTCAGCGGA TCCTATAGCT ACACCGATAT CTTCGTCAAT ATCCACAGCA
    ACAGTTATCC CAATTGTTGC AGCAACACCT ACGAATATCA CATCCGAACC GGTCATTGTC
    CAACCAATCA CTGCACAAGT TATAACCCAT TTGGCTACAC CATCGCCAGT TTCTCATTCG
    TTATCATCAA ATTCTTCTCC AAGTGCTACA ACTCATTCGA ATATGTCATC AATTCAATCA
    ACGACTAGCG TTCCTGGAAG ACGATTCACA GTTCAGCCCG TCTCTCAAGC AGAATCCGGA
    ATATCTTCGT CAATTTCTAC GCCTCATCCG GAACCGACGC CAGCAATCAC TTCGTGTCCT
    CCACCAGTTC CAAGTGTCCC ACCAGTGGTG TCCAATGGGA CATTGAACTT AGAAGTTGCT
    CCTAAACAGA CTCCATCAGC AACTAACCAA AATGTCGATA CACAACATTC ATCGTCAACA
    GCATCAACAG CTACATTAGT TTCGGAGACA CCTGCTACAG TACATGTTAC TCCGATCTCA
    GTGCCAGCTC CTGTTCAAGA ACCATTAGTC ATCGATCATC ATTCTGATGT ATTGACACAA
    TTGGATAGTG AGTTGAGAAA GTGTTTTCAG GTCAGTGGAG TATCACACTC GGCTTCACCT
    TCAACGGTAG TCGAGTCGCT CACCTCAATG ACTCCACAAA CAATTCCATT GGCTTGTCAA
    ACTGTGCCCG CATCGATCGG TCAAGCTCCA GCAGTCATCG CAGCTGCACA CGCGGCGTCA
    TTAATTCCAA ATGCAAGTGT TCCACAGTCA CCTAGTCGTC TTGATGCCGA AACTGGACTT
    GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG AAAATGGAAC AGGATCGACG AGAAGATATG
    GGAGATGATG CGATTGGCAC AACAACCACA GACGGAAAGG ACGAGATACC GATTGATACG
    TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA GTTATTCATG CGGATGGTAG AGAAACAACA
    CCAATGCCAC CGGATCATCC GGATTTAACA GATGCTTCAA CGCAACAACT CATCTCACCA
    TCTAACCCTG ATGTTTTGAC AACGATGTCG TCGGCTGTCG AAGGATCAGC ATCATCCACA
    ATGATAGAAG ACATTGATGC TTCAACATCA GCGGTAGACG CGTCAATGAT GAATTCGATG
    CCGCCAGGAG CTCAGAACTC TACAGATCAG ATACCAGCAG CGATGACACT GAGTATGGAT
    CAAGAATGTG CTCAATCGAT GACGTCATCG ATAACAAGAA ACACGACAGG CACCAAATTG
    GCGACTTTTG AGAATCTTGA AACAGCTCTA TCTTCCACAC TCGGAACACA CATTCGTCAA
    CCGAATGCAC CGTCTTCTCG AGATGAGACG ACGGCTCCGA TGACACCAAG TTTCACAAAT
    GAAAGAATTG GTGGTGGTGG AGGTGGTGGA GCAACATCAT TTAGTATAGG AACACCGCCC
    AGCCATAGTC CGTTCCCTGT TTCGGAGTGT GATTATGATT TGAAGGGTCA AATGGATTTG
    GAAAGCGAAG ATCCTGAGGT GATCCAGATG ATTGTTCGTC ATCGAATGGA ACAACACAAA
    CTTCTCGAAA AGCAACGTGT CGAGATTGAG CGTCTTCGTT CGAAAATTCG AGTTCCACGT
    GCAACTTCTG TGAATCCCGA AATGATTGGA GATGATGAAG CTGATACGAC GCTTACTGCT
    CTTCAAAGTG CATTAGGCAA TGCTTCATTG TCATTGCCAG CCAGTCCGCC ACCTAATACT
    GAGACAACGA AGGTCAACAC CACTGTAATT CCTTCCGATG TATTGGCGAC CCGAATGACA
    ATGTCTCAAT CGTCAACCAA ATCAAGCAAC GTCTCGGTAT CATCCCGTCA TCGCGACAAT
    CAGTCCGCTC CGCCACGTCA TCATCACCAT CAACCCCACC CTCCTCATCA TCCGCACCTC
    CAAAATCACT ATCATCCCCC ACAAAATCAT ACGTCAGCCA CTGCTCCTTG TCCATCGGCT
    ATGGTTCAAC TGCAAGCAGT GAGCAACAAC AACGTGAACC CTCTCCATCA GCCACCACAT
    CCAGTTTCCT CTCAGATCCC GCCACAGGCG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343987
    Clone ID Related Accession (Same CDS sequence) NM_001268439.2 , NM_001268439.1
    Accession Version NM_001268439.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268439.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGACAACGAA GGTCAACACC
    ACTGTAATTC CTTCCGATGT ATTGGCGACC CGAATGACAA TGTCTCAATC GTCAACCAAA
    TCAAGCAACG TCTCGGTATC ATCCCGTCAT CGCGACAATC AGTCCGCTCC GCCACGTCAT
    CATCACCATC AACCCCACCC TCCTCATCAT CCGCACCTCC AAAATCACTA TCATCCCCCA
    CAAAATCATA CGTCAGCCAC TGCTCCTTGT CCATCGGCTA TGGTTCAACT GCAAGCAGTG
    AGCAACAACA ACGTGAACCC TCTCCATCAG CCACCACATC CAGTTTCCTC TCAGATCCCG
    CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255368.1
    CDS316..5847
    Translation

    Target ORF information:

    RefSeq Version NM_001268439.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268439.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGACAACGAA GGTCAACACC
    ACTGTAATTC CTTCCGATGT ATTGGCGACC CGAATGACAA TGTCTCAATC GTCAACCAAA
    TCAAGCAACG TCTCGGTATC ATCCCGTCAT CGCGACAATC AGTCCGCTCC GCCACGTCAT
    CATCACCATC AACCCCACCC TCCTCATCAT CCGCACCTCC AAAATCACTA TCATCCCCCA
    CAAAATCATA CGTCAGCCAC TGCTCCTTGT CCATCGGCTA TGGTTCAACT GCAAGCAGTG
    AGCAACAACA ACGTGAACCC TCTCCATCAG CCACCACATC CAGTTTCCTC TCAGATCCCG
    CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343987
    Clone ID Related Accession (Same CDS sequence) NM_001268439.2 , NM_001268439.1
    Accession Version NM_001268439.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGACAACGAA GGTCAACACC
    ACTGTAATTC CTTCCGATGT ATTGGCGACC CGAATGACAA TGTCTCAATC GTCAACCAAA
    TCAAGCAACG TCTCGGTATC ATCCCGTCAT CGCGACAATC AGTCCGCTCC GCCACGTCAT
    CATCACCATC AACCCCACCC TCCTCATCAT CCGCACCTCC AAAATCACTA TCATCCCCCA
    CAAAATCATA CGTCAGCCAC TGCTCCTTGT CCATCGGCTA TGGTTCAACT GCAAGCAGTG
    AGCAACAACA ACGTGAACCC TCTCCATCAG CCACCACATC CAGTTTCCTC TCAGATCCCG
    CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255368.1
    CDS1..5532
    Translation

    Target ORF information:

    RefSeq Version NM_001268439.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    TGTTTTCAGG TCAGTGGAGT ATCACACTCG GCTTCACCTT CAACGGTAGT CGAGTCGCTC
    ACCTCAATGA CTCCACAAAC AATTCCATTG GCTTGTCAAA CTGTGCCCGC ATCGATCGGT
    CAAGCTCCAG CAGTCATCGC AGCTGCACAC GCGGCGTCAT TAATTCCAAA TGCAAGTGTT
    CCACAGTCAC CTAGTCGTCT TGATGCCGAA ACTGGACTTG CCGGTCTTCA CGAGAAGCTT
    GAAGCGCTGA AAATGGAACA GGATCGACGA GAAGATATGG GAGATGATGC GATTGGCACA
    ACAACCACAG ACGGAAAGGA CGAGATACCG ATTGATACGT TAAAAGGGCT CGCGGAAGCA
    TTAGGAAAAG TTATTCATGC GGATGGTAGA GAAACAACAC CAATGCCACC GGATCATCCG
    GATTTAACAG ATGCTTCAAC GCAACAACTC ATCTCACCAT CTAACCCTGA TGTTTTGACA
    ACGATGTCGT CGGCTGTCGA AGGATCAGCA TCATCCACAA TGATAGAAGA CATTGATGCT
    TCAACATCAG CGGTAGACGC GTCAATGATG AATTCGATGC CGCCAGGAGC TCAGAACTCT
    ACAGATCAGA TACCAGCAGC GATGACACTG AGTATGGATC AAGAATGTGC TCAATCGATG
    ACGTCATCGA TAACAAGAAA CACGACAGGC ACCAAATTGG CGACTTTTGA GAATCTTGAA
    ACAGCTCTAT CTTCCACACT CGGAACACAC ATTCGTCAAC CGAATGCACC GTCTTCTCGA
    GATGAGACGA CGGCTCCGAT GACACCAAGT TTCACAAATG AAAGAATTGG TGGTGGTGGA
    GGTGGTGGAG CAACATCATT TAGTATAGGA ACACCGCCCA GCCATAGTCC GTTCCCTGTT
    TCGGAGTGTG ATTATGATTT GAAGGGTCAA ATGGATTTGG AAAGCGAAGA TCCTGAGGTG
    ATCCAGATGA TTGTTCGTCA TCGAATGGAA CAACACAAAC TTCTCGAAAA GCAACGTGTC
    GAGATTGAGC GTCTTCGTTC GAAAATTCGA GTTCCACGTG CAACTTCTGT GAATCCCGAA
    ATGATTGGAG ATGATGAAGC TGATACGACG CTTACTGCTC TTCAAAGTGC ATTAGGCAAT
    GCTTCATTGT CATTGCCAGC CAGTCCGCCA CCTAATACTG AGACAACGAA GGTCAACACC
    ACTGTAATTC CTTCCGATGT ATTGGCGACC CGAATGACAA TGTCTCAATC GTCAACCAAA
    TCAAGCAACG TCTCGGTATC ATCCCGTCAT CGCGACAATC AGTCCGCTCC GCCACGTCAT
    CATCACCATC AACCCCACCC TCCTCATCAT CCGCACCTCC AAAATCACTA TCATCCCCCA
    CAAAATCATA CGTCAGCCAC TGCTCCTTGT CCATCGGCTA TGGTTCAACT GCAAGCAGTG
    AGCAACAACA ACGTGAACCC TCTCCATCAG CCACCACATC CAGTTTCCTC TCAGATCCCG
    CCACAGGCGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343988
    Clone ID Related Accession (Same CDS sequence) NM_001268441.1 , NM_001268441.2
    Accession Version NM_001268441.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255370.1
    CDS1..5523
    Translation

    Target ORF information:

    RefSeq Version NM_001268441.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343988
    Clone ID Related Accession (Same CDS sequence) NM_001268441.1 , NM_001268441.2
    Accession Version NM_001268441.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268441.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255370.1
    CDS316..5838
    Translation

    Target ORF information:

    RefSeq Version NM_001268441.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGCAACAACT CATCTCACCA TCTAACCCTG ATGTTTTGAC AACGATGTCG
    TCGGCTGTCG AAGGATCAGC ATCATCCACA ATGATAGAAG ACATTGATGC TTCAACATCA
    GCGGTAGACG CGTCAATGAT GAATTCGATG CCGCCAGGAG CTCAGAACTC TACAGATCAG
    ATACCAGCAG CGATGACACT GAGTATGGAT CAAGAATGTG CTCAATCGAT GACGTCATCG
    ATAACAAGAA ACACGACAGG CACCAAATTG GCGACTTTTG AGAATCTTGA AACAGCTCTA
    TCTTCCACAC TCGGAACACA CATTCGTCAA CCGAATGCAC CGTCTTCTCG AGATGAGACG
    ACGGCTCCGA TGACACCAAG TTTCACAAAT GAAAGAATTG GTGGTGGTGG AGGTGGTGGA
    GCAACATCAT TTAGTATAGG AACACCGCCC AGCCATAGTC CGTTCCCTGT TTCGGAGTGT
    GATTATGATT TGAAGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343989
    Clone ID Related Accession (Same CDS sequence) NM_001268442.1 , NM_001268442.2
    Accession Version NM_001268442.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ACAACGAAGG TCAACACCAC TGTAATTCCT
    TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC AAGCAACGTC
    TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA TCACCATCAA
    CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA AAATCATACG
    TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG CAACAACAAC
    GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC ACAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255371.1
    CDS1..5040
    Translation

    Target ORF information:

    RefSeq Version NM_001268442.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ACAACGAAGG TCAACACCAC TGTAATTCCT
    TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC AAGCAACGTC
    TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA TCACCATCAA
    CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA AAATCATACG
    TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG CAACAACAAC
    GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC ACAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343989
    Clone ID Related Accession (Same CDS sequence) NM_001268442.1 , NM_001268442.2
    Accession Version NM_001268442.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268442.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ACAACGAAGG TCAACACCAC TGTAATTCCT
    TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC AAGCAACGTC
    TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA TCACCATCAA
    CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA AAATCATACG
    TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG CAACAACAAC
    GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC ACAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255371.1
    CDS316..5355
    Translation

    Target ORF information:

    RefSeq Version NM_001268442.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATTTCAGT GGACTTCAAA AAAGAGACCA GATGATCCAA CGACATCATC TCCATCGACA
    GTTTCAATTA GTAATGCATT GGAAAATTCA ACACCTTCAT TAAACAATGT TTCTTCGATC
    ACAAACTCTT CATCACCATT TTCTTTATCG TCAGCTGCAA CATCGACAGC TTCTGCCATA
    ATCCCATTTA CCTCAAATGT AGCTACAAAT CATCCACATC TTAATCACCA CGTGTCGAGA
    ATACCACAGG CGATTGTAAC AGGAGGGACA AATGGTTCAT TACCTCCATT ATTAATATCA
    CCGACGTCAG CAGCAGCCGC AACGCCGTTG ATTTCAGGAA AAGCGGGTCC AATGTCACCA
    TCAACGGGAA GTCCAATTAA TGTGGCTGCT ACAGTATTAC AGAATGCAGT TTCAAGTCCA
    CAACATAGTA TTTTTGATAG ATCTAGGTTA AATAAAATTC CACCAAACAC ATCATTAGCA
    TCGTCTTCGT CTCCGAGTGA TGCTGCTAAC AATGATAAAC CTATACAACA ACGTCATAGT
    ATTTTATCGA ATGTTCGAAC TCTCACTCAA GCAATGGTAA ACGATGGACC ACGAACGTTG
    ACTGGAGATG ATATGGACAA GATGGTCAGT GAAGAGGAAA GAGCACGAAA AGAGCAAGAG
    AAGCGAGAAG AAGAAGAGAA AGCAGCACGG AGAATTGATG TTGAAGATGA TTTTGATGCG
    CAAGAAAAAC CCATTGATAA GAGTAAAAAT GGTCGATTCT TAAAGTTCGA CGAAGAACTT
    GGTCGCGGAT CGTTCAAGAC AGTGTTTCGA GGGTTGGACA CTGAAACCGG AGTGGCCGTT
    GCGTGGTGTG AGCTTCAGGA GAGTAAACTG AATAAGACTG AAAGGCAACG TTTTCGAGAA
    GAAGCTGAAA TGCTCAAGGA TCTGCAACAT CCGAACATCG TACGATTCTA CGATTATTGG
    GAGAGTGCGG ATTTGTGTGG AAAACGGAAA TATATTGTGC TTGTAACCGA ATTGATGACT
    TCAGGAACAT TAAAAATGTA TTTGAAGCGA TTTAAACGGA TAAACATAAA GGTATTGAAA
    TCTTGGTGCA GACAAATTCT GAAAGGGCTT TCATTTCTTC ACACACGTAA TCCACCTGTA
    ATTCATCGTG ATCTCAAGTG TGATAATATC TTTATCACTG GTACAACTGG TAGTGTAAAG
    ATTGGAGATC TTGGATTGGC AACTCTTAAA AATAAATCAT TCGCGAAATC TGTCATTGGA
    ACTCCAGAAT TCATGGCACC GGAAATGTAC GAAGAGATGT ATGATGAGAG TGTAGATGTC
    TACGCGTTTG GAATGTGTCT TCTTGAAATG GTTACCGGAG AATATCCATA TTCTGAGTGT
    ATGAATCCAG CGACAATCTA CAGAAAAGTG ATATCAGGTG TCAAACCAGA ATGTTTCTCA
    AGAATTCCTG CACAATATCC TGAAATACGT GAGATAATCG ATCGGTGTAT TCGTGTGAGA
    CGAGAAGAGA GAAGTACCGT GAAGCAATTG CTCGTTGACG ATTTCTTCAC TCCAGAAGAT
    CTCATCGGAA TTCGTGTTGA AATTAAGAAT CGGGATGCTG ATCTCAATGA TCTTAACGTG
    GAAATTCAAA TGCAACTCCG AGTTTACGAT GAGAAAAAGA GAAAACAATA TCGGTTCAAG
    GAGAACGAAG GACTTCAATT TGCATTTGAT ATTGAAAATG ACAGCCCAGA TGAAGTTGTT
    CAACAGATGA TCGAGCAACA ACATATTCCA GATGAGGATA CTCGAATGAT AACAAAGTTG
    ATAAAAGACA AAGTGGATGC GTTCAGAAGA GATCGAGATC ATCGTTTATT GGAGATTAAA
    AGAGCAAAAG AGGAGGAAGA AAGGATACGA GAAGAGGCTG AGATTAAAGA GGAATTGAGG
    TTGAGAGCTG AAGCTAAAGA AAAGGAAAAG GAGAGGTTGG AGAAAGAACG ATTAGAAAAA
    AAAGCTGCAG CTGCGGCCGC AGCCAATCCG AATCCTACAC CAATTCCACC AACTCCAGCA
    ACACCACACT CTTCTGCCCA ACAACAGCCT ATTCCACCAC CACTATCAAC TCAAACTTCG
    GCTGAAATAC AGCAGTCTGC TCAACAGCCA TCTGTACCTG TAACTATGAT CGCAAATATC
    CCAGCTATGT CGCCAACATC AGCTCAACCA CAACCTGTTT TATCACCAAC AAGTGCAGCG
    GTTCCAGTTC CAACGACAAT GATTCATGTT CCAAAACCTA GCGAGATTCC AGTGCAGAAT
    GTAGCGACGA CTGCAGCCCC GGTTGCGGCT AACAATGTAC CACCATCACC AGCTCCATTC
    AAAACAGAAG ATATCCAAAC TCCCACACTT GCCCAGAATA CGGTTCCACG AACAATCTCC
    ACTGATGCTT CTGGACTTGT CATCAATACT CCTGCATCAA TAGCATCACC ATCACCTGCT
    CCTTCGGCTA CTGATGTTGC TTCAACAACG GCTCCAGTCA CCCCTGCTCC AACTCCAACA
    ACAACAACGG ACGGTGGTGC AGCAGCAGCT TCAACAACAA CTGAAAACAA GGAAGAAAAG
    CGAAAAAGCA ACAAAAGAAA AGTGGTAATG GAGATTTTGG GATGTGATGA ATCGAGGAAT
    TTTGCATTGG TCTCGTGTCG ACTTGACACT TCTCATAAAT CTGTTACCTT CCAATTTGCA
    CCAGGAACTG ACAAACCATG CACAATTGCA ACGAAACTTT TGGCTGAAGA TTGTCTTTTA
    AAAGTTCATG TTCATATTGT TGAAGCACAA TTGGGAGAAG TTATTCAATT GATTAATTCT
    GATGGAAAGA AAGGTGTTGG TACTAAATTA GCGACGGTAT TGGATCCAAA TAGTACTGAA
    CCACCAACTA TCACTGCTGT CATGCCAAAG GATTCCTCTG CCGCAACTGC TTCTAATACT
    AAGCCTAAGA TTGAAATCGA GAAAACGCCA CCAACGAGAG ATGCTTCCCA AGAGCCGAAT
    AATGTTCAGG TTACGAACGT ACGCAAAGTT TCACAAGAAT CAAATGCCGA AAGTGTTCAA
    TCAATTCCTC GTCCAGGTGG AATCATTGTG ATGTCCCCAA CAAATCAAAC AGATTCGGCT
    CCGCCCCCAA CTGGAGCTGC AGCAAAACCA TCACGATTCC AAGTAACAAA GTCAGCGGAT
    CCTATAGCTA CACCGATATC TTCGTCAATA TCCACAGCAA CAGTTATCCC AATTGTTGCA
    GCAACACCTA CGAATATCAC ATCCGAACCG GTCATTGTCC AACCAATCAC TGCACAAGTT
    ATAACCCATT TGGCTACACC ATCGCCAGTT TCTCATTCGT TATCATCAAA TTCTTCTCCA
    AGTGCTACAA CTCATTCGAA TATGTCATCA ATTCAATCAA CGACTAGCGT TCCTGGAAGA
    CGATTCACAG TTCAGCCCGT CTCTCAAGCA GAATCCGGAA TATCTTCGTC AATTTCTACG
    CCTCATCCGG AACCGACGCC AGCAATCACT TCGTGTCCTC CACCAGTTCC AAGTGTCCCA
    CCAGTGGTGT CCAATGGGAC ATTGAACTTA GAAGTTGCTC CTAAACAGAC TCCATCAGCA
    ACTAACCAAA ATGTCGATAC ACAACATTCA TCGTCAACAG CATCAACAGC TACATTAGTT
    TCGGAGACAC CTGCTACAGT ACATGTTACT CCGATCTCAG TGCCAGCTCC TGTTCAAGAA
    CCATTAGTCA TCGATCATCA TTCTGATGTA TTGACACAAT TGGATAGTGA GTTGAGAAAG
    GTCAGTGGAG TATCACACTC GGCTTCACCT TCAACGGTAG TCGAGTCGCT CACCTCAATG
    ACTCCACAAA CAATTCCATT GGCTTGTCAA ACTGTGCCCG CATCGATCGG TCAAGCTCCA
    GCAGTCATCG CAGCTGCACA CGCGGCGTCA TTAATTCCAA ATGCAAGTGT TCCACAGTCA
    CCTAGTCGTC TTGATGCCGA AACTGGACTT GCCGGTCTTC ACGAGAAGCT TGAAGCGCTG
    AAAATGGAAC AGGATCGACG AGAAGATATG GGAGATGATG CGATTGGCAC AACAACCACA
    GACGGAAAGG ACGAGATACC GATTGATACG TTAAAAGGGC TCGCGGAAGC ATTAGGAAAA
    GTTATTCATG CGGATGGTAG AGAAACAACA CCAATGCCAC CGGATCATCC GGATTTAACA
    GATGCTTCAA CGGGTCAAAT GGATTTGGAA AGCGAAGATC CTGAGGTGAT CCAGATGATT
    GTTCGTCATC GAATGGAACA ACACAAACTT CTCGAAAAGC AACGTGTCGA GATTGAGCGT
    CTTCGTTCGA AAATTCGAGT TCCACGTGCA ACTTCTGTGA ATCCCGAAAT GATTGGAGAT
    GATGAAGCTG ATACGACGCT TACTGCTCTT CAAAGTGCAT TAGGCAATGC TTCATTGTCA
    TTGCCAGCCA GTCCGCCACC TAATACTGAG ACAACGAAGG TCAACACCAC TGTAATTCCT
    TCCGATGTAT TGGCGACCCG AATGACAATG TCTCAATCGT CAACCAAATC AAGCAACGTC
    TCGGTATCAT CCCGTCATCG CGACAATCAG TCCGCTCCGC CACGTCATCA TCACCATCAA
    CCCCACCCTC CTCATCATCC GCACCTCCAA AATCACTATC ATCCCCCACA AAATCATACG
    TCAGCCACTG CTCCTTGTCC ATCGGCTATG GTTCAACTGC AAGCAGTGAG CAACAACAAC
    GTGAACCCTC TCCATCAGCC ACCACATCCA GTTTCCTCTC AGATCCCGCC ACAGGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343990
    Clone ID Related Accession (Same CDS sequence) NM_001268444.2 , NM_001268444.1
    Accession Version NM_001268444.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268444.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255373.1
    CDS316..5358
    Translation

    Target ORF information:

    RefSeq Version NM_001268444.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343990
    Clone ID Related Accession (Same CDS sequence) NM_001268444.2 , NM_001268444.1
    Accession Version NM_001268444.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Serine/threonine-protein kinase WNK
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255373.1
    CDS1..5043
    Translation

    Target ORF information:

    RefSeq Version NM_001268444.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Serine/threonine-protein kinase WNK (wnk-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCCTGATT CTATAACAAA CGGTGGACGA CCACCAGCAC CTCCAAGCTC TGTGAGCTCA 
    ACAACAGCAT CGACAACTGG TAATTTTGGT ACTCGACGTC GTTTAGTGAA TCGTATCAAG
    AAAGTCGATG AACTACATCC AGCACAAGAG AATCCCACAA TGGGATCACA CTGGTTGTCC
    GAGCAAGAAA GATCACGACT TGAAGCTGTT CAACAGGATT GGCGAAGAAC TCGACCAATG
    AAATGGACTT CAAAAAAGAG ACCAGATGAT CCAACGACAT CATCTCCATC GACAGTTTCA
    ATTAGTAATG CATTGGAAAA TTCAACACCT TCATTAAACA ATGTTTCTTC GATCACAAAC
    TCTTCATCAC CATTTTCTTT ATCGTCAGCT GCAACATCGA CAGCTTCTGC CATAATCCCA
    TTTACCTCAA ATGTAGCTAC AAATCATCCA CATCTTAATC ACCACGTGTC GAGAATACCA
    CAGGCGATTG TAACAGGAGG GACAAATGGT TCATTACCTC CATTATTAAT ATCACCGACG
    TCAGCAGCAG CCGCAACGCC GTTGATTTCA GGAAAAGCGG GTCCAATGTC ACCATCAACG
    GGAAGTCCAA TTAATGTGGC TGCTACAGTA TTACAGAATG CAGTTTCAAG TCCACAACAT
    AGTATTTTTG ATAGATCTAG GTTAAATAAA ATTCCACCAA ACACATCATT AGCATCGTCT
    TCGTCTCCGA GTGATGCTGC TAACAATGAT AAACCTATAC AACAACGTCA TAGTATTTTA
    TCGAATGTTC GAACTCTCAC TCAAGCAATG GTAAACGATG GACCACGAAC GTTGACTGGA
    GATGATATGG ACAAGATGGT CAGTGAAGAG GAAAGAGCAC GAAAAGAGCA AGAGAAGCGA
    GAAGAAGAAG AGAAAGCAGC ACGGAGAATT GATGTTGAAG ATGATTTTGA TGCGCAAGAA
    AAACCCATTG ATAAGAGTAA AAATGGTCGA TTCTTAAAGT TCGACGAAGA ACTTGGTCGC
    GGATCGTTCA AGACAGTGTT TCGAGGGTTG GACACTGAAA CCGGAGTGGC CGTTGCGTGG
    TGTGAGCTTC AGGAGAGTAA ACTGAATAAG ACTGAAAGGC AACGTTTTCG AGAAGAAGCT
    GAAATGCTCA AGGATCTGCA ACATCCGAAC ATCGTACGAT TCTACGATTA TTGGGAGAGT
    GCGGATTTGT GTGGAAAACG GAAATATATT GTGCTTGTAA CCGAATTGAT GACTTCAGGA
    ACATTAAAAA TGTATTTGAA GCGATTTAAA CGGATAAACA TAAAGGTATT GAAATCTTGG
    TGCAGACAAA TTCTGAAAGG GCTTTCATTT CTTCACACAC GTAATCCACC TGTAATTCAT
    CGTGATCTCA AGTGTGATAA TATCTTTATC ACTGGTACAA CTGGTAGTGT AAAGATTGGA
    GATCTTGGAT TGGCAACTCT TAAAAATAAA TCATTCGCGA AATCTGTCAT TGGAACTCCA
    GAATTCATGG CACCGGAAAT GTACGAAGAG ATGTATGATG AGAGTGTAGA TGTCTACGCG
    TTTGGAATGT GTCTTCTTGA AATGGTTACC GGAGAATATC CATATTCTGA GTGTATGAAT
    CCAGCGACAA TCTACAGAAA AGTGATATCA GGTGTCAAAC CAGAATGTTT CTCAAGAATT
    CCTGCACAAT ATCCTGAAAT ACGTGAGATA ATCGATCGGT GTATTCGTGT GAGACGAGAA
    GAGAGAAGTA CCGTGAAGCA ATTGCTCGTT GACGATTTCT TCACTCCAGA AGATCTCATC
    GGAATTCGTG TTGAAATTAA GAATCGGGAT GCTGATCTCA ATGATCTTAA CGTGGAAATT
    CAAATGCAAC TCCGAGTTTA CGATGAGAAA AAGAGAAAAC AATATCGGTT CAAGGAGAAC
    GAAGGACTTC AATTTGCATT TGATATTGAA AATGACAGCC CAGATGAAGT TGTTCAACAG
    ATGATCGAGC AACAACATAT TCCAGATGAG GATACTCGAA TGATAACAAA GTTGATAAAA
    GACAAAGTGG ATGCGTTCAG AAGAGATCGA GATCATCGTT TATTGGAGAT TAAAAGAGCA
    AAAGAGGAGG AAGAAAGGAT ACGAGAAGAG GCTGAGATTA AAGAGGAATT GAGGTTGAGA
    GCTGAAGCTA AAGAAAAGGA AAAGGAGAGG TTGGAGAAAG AACGATTAGA AAAAAAAGCT
    GCAGCTGCGG CCGCAGCCAA TCCGAATCCT ACACCAATTC CACCAACTCC AGCAACACCA
    CACTCTTCTG CCCAACAACA GCCTATTCCA CCACCACTAT CAACTCAAAC TTCGGCTGAA
    ATACAGCAGT CTGCTCAACA GCCATCTGTA CCTGTAACTA TGATCGCAAA TATCCCAGCT
    ATGTCGCCAA CATCAGCTCA ACCACAACCT GTTTTATCAC CAACAAGTGC AGCGGTTCCA
    GTTCCAACGA CAATGATTCA TGTTCCAAAA CCTAGCGAGA TTCCAGTGCA GAATGTAGCG
    ACGACTGCAG CCCCGGTTGC GGCTAACAAT GTACCACCAT CACCAGCTCC ATTCAAAACA
    GAAGATATCC AAACTCCCAC ACTTGCCCAG AATACGGTTC CACGAACAAT CTCCACTGAT
    GCTTCTGGAC TTGTCATCAA TACTCCTGCA TCAATAGCAT CACCATCACC TGCTCCTTCG
    GCTACTGATG TTGCTTCAAC AACGGCTCCA GTCACCCCTG CTCCAACTCC AACAACAACA
    ACGGACGGTG GTGCAGCAGC AGCTTCAACA ACAACTGAAA ACAAGGAAGA AAAGCGAAAA
    AGCAACAAAA GAAAAGTGGT AATGGAGATT TTGGGATGTG ATGAATCGAG GAATTTTGCA
    TTGGTCTCGT GTCGACTTGA CACTTCTCAT AAATCTGTTA CCTTCCAATT TGCACCAGGA
    ACTGACAAAC CATGCACAAT TGCAACGAAA CTTTTGGCTG AAGATTGTCT TTTAAAAGTT
    CATGTTCATA TTGTTGAAGC ACAATTGGGA GAAGTTATTC AATTGATTAA TTCTGATGGA
    AAGAAAGGTG TTGGTACTAA ATTAGCGACG GTATTGGATC CAAATAGTAC TGAACCACCA
    ACTATCACTG CTGTCATGCC AAAGGATTCC TCTGCCGCAA CTGCTTCTAA TACTAAGCCT
    AAGATTGAAA TCGAGAAAAC GCCACCAACG AGAGATGCTT CCCAAGAGCC GAATAATGTT
    CAGGTTACGA ACGTACGCAA AGTTTCACAA GAATCAAATG CCGAAAGTGT TCAATCAATT
    CCTCGTCCAG GTGGAATCAT TGTGATGTCC CCAACAAATC AAACAGATTC GGCTCCGCCC
    CCAACTGGAG CTGCAGCAAA ACCATCACGA TTCCAAGTAA CAAAGTCAGC GGATCCTATA
    GCTACACCGA TATCTTCGTC AATATCCACA GCAACAGTTA TCCCAATTGT TGCAGCAACA
    CCTACGAATA TCACATCCGA ACCGGTCATT GTCCAACCAA TCACTGCACA AGTTATAACC
    CATTTGGCTA CACCATCGCC AGTTTCTCAT TCGTTATCAT CAAATTCTTC TCCAAGTGCT
    ACAACTCATT CGAATATGTC ATCAATTCAA TCAACGACTA GCGTTCCTGG AAGACGATTC
    ACAGTTCAGC CCGTCTCTCA AGCAGAATCC GGAATATCTT CGTCAATTTC TACGCCTCAT
    CCGGAACCGA CGCCAGCAAT CACTTCGTGT CCTCCACCAG TTCCAAGTGT CCCACCAGTG
    GTGTCCAATG GGACATTGAA CTTAGAAGTT GCTCCTAAAC AGACTCCATC AGCAACTAAC
    CAAAATGTCG ATACACAACA TTCATCGTCA ACAGCATCAA CAGCTACATT AGTTTCGGAG
    ACACCTGCTA CAGTACATGT TACTCCGATC TCAGTGCCAG CTCCTGTTCA AGAACCATTA
    GTCATCGATC ATCATTCTGA TGTATTGACA CAATTGGATA GTGAGTTGAG AAAGTGTTTT
    CAGGTCAGTG GAGTATCACA CTCGGCTTCA CCTTCAACGG TAGTCGAGTC GCTCACCTCA
    ATGACTCCAC AAACAATTCC ATTGGCTTGT CAAACTGTGC CCGCATCGAT CGGTCAAGCT
    CCAGCAGTCA TCGCAGCTGC ACACGCGGCG TCATTAATTC CAAATGCAAG TGTTCCACAG
    TCACCTAGTC GTCTTGATGC CGAAACTGGA CTTGCCGGTC TTCACGAGAA GCTTGAAGCG
    CTGAAAATGG AACAGGATCG ACGAGAAGAT ATGGGAGATG ATGCGATTGG CACAACAACC
    ACAGACGGAA AGGACGAGAT ACCGATTGAT ACGTTAAAAG GGCTCGCGGA AGCATTAGGA
    AAAGTTATTC ATGCGGATGG TAGAGAAACA ACACCAATGC CACCGGATCA TCCGGATTTA
    ACAGATGCTT CAACGGGTCA AATGGATTTG GAAAGCGAAG ATCCTGAGGT GATCCAGATG
    ATTGTTCGTC ATCGAATGGA ACAACACAAA CTTCTCGAAA AGCAACGTGT CGAGATTGAG
    CGTCTTCGTT CGAAAATTCG AGTTCCACGT GCAACTTCTG TGAATCCCGA AATGATTGGA
    GATGATGAAG CTGATACGAC GCTTACTGCT CTTCAAAGTG CATTAGGCAA TGCTTCATTG
    TCATTGCCAG CCAGTCCGCC ACCTAATACT GAGACAACGA AGGTCAACAC CACTGTAATT
    CCTTCCGATG TATTGGCGAC CCGAATGACA ATGTCTCAAT CGTCAACCAA ATCAAGCAAC
    GTCTCGGTAT CATCCCGTCA TCGCGACAAT CAGTCCGCTC CGCCACGTCA TCATCACCAT
    CAACCCCACC CTCCTCATCA TCCGCACCTC CAAAATCACT ATCATCCCCC ACAAAATCAT
    ACGTCAGCCA CTGCTCCTTG TCCATCGGCT ATGGTTCAAC TGCAAGCAGT GAGCAACAAC
    AACGTGAACC CTCTCCATCA GCCACCACAT CCAGTTTCCT CTCAGATCCC GCCACAGGCG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.