isp-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	isp-1
Entrez Gene ID	177609
Full Name	Cytochrome b-c1 complex subunit Rieske, mitochondrial
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_068960.3	NP_501361.1	Cytochrome b-c1 complex subunit Rieske, mitochondrial
NM_068960.4	NP_501361.1	Cytochrome b-c1 complex subunit Rieske, mitochondrial

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100334857	XP_003199036.1
Drosophila melanogaster (fruit fly)	RFeSP	NP_722715.1
Kluyveromyces lactis	KLLA0E22573g	XP_454973.1
Schizosaccharomyces pombe (fission yeast)	rip1	NP_595941.1
Neurospora crassa	NCU06606	XP_962348.1
Oryza sativa (rice)	Os04g0398500	NP_001052672.1
Bos taurus (cattle)	UQCRFS1	NP_777238.1
Mus musculus (house mouse)	Uqcrfs1	NP_079986.1
	UQCRFS1	XP_001101289.1
Canis lupus familiaris (dog)	UQCRFS1	XP_533711.1
Rattus norvegicus (Norway rat)	Uqcrfs1	NP_001008888.1
Gallus gallus (chicken)	UQCRFS1	NP_001005843.1
Danio rerio (zebrafish)	uqcrfs1	NP_001096664.1
Arabidopsis thaliana (thale cress)	AT5G13440	NP_196848.1
Arabidopsis thaliana (thale cress)	AT5G13430	NP_568288.1
Xenopus tropicalis (tropical clawed frog)	uqcrfs1	NP_988911.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008955	XP_319708.4
Caenorhabditis elegans (roundworm)	isp-1	NP_501361.1
Eremothecium gossypii	AGOS_AEL067W	NP_984794.1
Magnaporthe oryzae (rice blast fungus)	MGG_16866	XP_003712616.1
Homo sapiens (human)	UQCRFS1	NP_005994.2
Pan troglodytes (chimpanzee)	UQCRFS1	XP_001148728.1
Saccharomyces cerevisiae (baker's yeast)	RIP1	NP_010890.3
Oryza sativa (rice)	Os02g0520800	NP_001046975.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gating and control processes influence the function of ISP-1 in mediating pleiotropic phenotypes including developmental rate, movement, sensitivity to stress, and longevity.
Title: Tether mutations that restore function and suppress pleiotropic phenotypes of the C. elegans isp-1(qm150) Rieske iron-sulfur protein.

stress response, and/or fecundity of isp-1(qm150) Mit mutants relative to wild-type animalsstress response, and/or fecundity of isp-1(qm150) Mit mutants relative to wild-type animals
Title: TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants.

ROS production by mitochondrial mutants and found that isp-1 and nuo-6 mutants have increased generation of the superoxide anion but not increased levels of other ROS and that this increase is necessary and sufficient for longevity.
Title: A mitochondrial superoxide signal triggers increased longevity in Caenorhabditis elegans.

found that the lifespan increase induced by nuo-6(RNAi) is fully additive to that induced by isp-1(qm150), and the increase induced by isp-1(RNAi) is fully additive to that induced by nuo-6(qm200)
Title: Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans.

The following isp-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the isp-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa343295	NM_068960.4 Latest version!	Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OCa343295	NM_068960.3	Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

isp-1 ( NM_068960.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501361.1 Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), partial mRNA.

Price & Availability↑

CloneID	OCa343295
Clone ID Related Accession (Same CDS sequence)	NM_068960.3 , NM_068960.4
Accession Version	NM_068960.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Cytochrome b-c1 complex subunit Rieske, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_068960.2. COMPLETENESS: incomplete on both ends.

ATGGCTTCTC TTGCTAGATC TGGAGGTTTT GCAGTCAAAG TGATCAGCGG ATCCCAGCAA 
CATGTAAACT TTGTTGCCGC AACACCAGCC GCCGATAATG TCGCTGCTTT CCGCGATGTT 
CCAGCTGAGT TCTCCACGGC TGAAGCTCGT CAAGCCGCTG TTAGTCACAG CACCGGAGGT 
GTCTTCACCA ATGGACTTGC TGTCAAGGGA ATCAATGTCA CCTCTCGCCG TTTGGCTCAC 
ACTGATGTGA CATTCCCCGA TATGTCAAAC TATCGTCGTG ATTCTACAAA GAACACTCAA 
GTGCCAGCCC GTGACACCGA GGATCAACGT CGTGCTCTTC CAACTGCTCT GTATTATGGA 
GCTGGAGGAG TTCTATCTTT GTGGGCCGGA AAGGAAGTTG TACAGACTCT TGTTTCTTAC 
AAAGCTATGG CAGCTGATCA ACGTGCCTTG GCTTCGATCG AAATCAACAT GGCTGATATT 
CCAGAAGGAA AAACAAAGAC ATTCGAATGG CGTGGAAAGC CAGTCTTCGT CAAACATCGT 
ACCAAGGCTG AGATTGCCAA GGAAAAGGCC GTCCCTGTCG CCGATCTCCG TCATCCACAA 
CATGACGACG AACGTGTGCA GAAAGATGAA TGGTCCGTTG TCATTGGAGT GTGCACCCAT 
CTTGGATGTG TCCCAATTGC CGATGCTGGA GATTACGGAG GTTATTACTG CCCTTGTCAC 
GGATCTCACT ACGACGCTTC TGGACGTATT AGAAAGGGAC CAGCTCCATT GAATCTTCAC 
GTACCAGCAT ACTCGTTCAA GGATGCCACC ATCGTCATTG GATCGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501361.1
CDS	1..831
Translation	MASLARSGGFAVKVISGSQQHVNFVAATPAADNVAAFRDVPAEFSTAEARQAAVSHSTGGVFTNGLAVKGINVTSRRLAHTDVTFPDMSNYRRDSTKNTQVPARDTEDQRRALPTALYYGAGGVLSLWAGKEVVQTLVSYKAMAADQRALASIEINMADIPEGKTKTFEWRGKPVFVKHRTKAEIAKEKAVPVADLRHPQHDDERVQKDEWSVVIGVCTHLGCVPIADAGDYGGYYCPCHGSHYDASGRIRKGPAPLNLHVPAYSFKDATIVIGSS

Target ORF information:

RefSeq Version	NM_068960.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068960.3

ORF Insert Sequence:

ATGGCTTCTC TTGCTAGATC TGGAGGTTTT GCAGTCAAAG TGATCAGCGG ATCCCAGCAA 
CATGTAAACT TTGTTGCCGC AACACCAGCC GCCGATAATG TCGCTGCTTT CCGCGATGTT 
CCAGCTGAGT TCTCCACGGC TGAAGCTCGT CAAGCCGCTG TTAGTCACAG CACCGGAGGT 
GTCTTCACCA ATGGACTTGC TGTCAAGGGA ATCAATGTCA CCTCTCGCCG TTTGGCTCAC 
ACTGATGTGA CATTCCCCGA TATGTCAAAC TATCGTCGTG ATTCTACAAA GAACACTCAA 
GTGCCAGCCC GTGACACCGA GGATCAACGT CGTGCTCTTC CAACTGCTCT GTATTATGGA 
GCTGGAGGAG TTCTATCTTT GTGGGCCGGA AAGGAAGTTG TACAGACTCT TGTTTCTTAC 
AAAGCTATGG CAGCTGATCA ACGTGCCTTG GCTTCGATCG AAATCAACAT GGCTGATATT 
CCAGAAGGAA AAACAAAGAC ATTCGAATGG CGTGGAAAGC CAGTCTTCGT CAAACATCGT 
ACCAAGGCTG AGATTGCCAA GGAAAAGGCC GTCCCTGTCG CCGATCTCCG TCATCCACAA 
CATGACGACG AACGTGTGCA GAAAGATGAA TGGTCCGTTG TCATTGGAGT GTGCACCCAT 
CTTGGATGTG TCCCAATTGC CGATGCTGGA GATTACGGAG GTTATTACTG CCCTTGTCAC 
GGATCTCACT ACGACGCTTC TGGACGTATT AGAAAGGGAC CAGCTCCATT GAATCTTCAC 
GTACCAGCAT ACTCGTTCAA GGATGCCACC ATCGTCATTG GATCGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

isp-1 ( NM_068960.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501361.1 Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), mRNA.

Price & Availability↑

CloneID	OCa343295
Clone ID Related Accession (Same CDS sequence)	NM_068960.3 , NM_068960.4
Accession Version	NM_068960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Cytochrome b-c1 complex subunit Rieske, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_068960.3.

ATGGCTTCTC TTGCTAGATC TGGAGGTTTT GCAGTCAAAG TGATCAGCGG ATCCCAGCAA 
CATGTAAACT TTGTTGCCGC AACACCAGCC GCCGATAATG TCGCTGCTTT CCGCGATGTT 
CCAGCTGAGT TCTCCACGGC TGAAGCTCGT CAAGCCGCTG TTAGTCACAG CACCGGAGGT 
GTCTTCACCA ATGGACTTGC TGTCAAGGGA ATCAATGTCA CCTCTCGCCG TTTGGCTCAC 
ACTGATGTGA CATTCCCCGA TATGTCAAAC TATCGTCGTG ATTCTACAAA GAACACTCAA 
GTGCCAGCCC GTGACACCGA GGATCAACGT CGTGCTCTTC CAACTGCTCT GTATTATGGA 
GCTGGAGGAG TTCTATCTTT GTGGGCCGGA AAGGAAGTTG TACAGACTCT TGTTTCTTAC 
AAAGCTATGG CAGCTGATCA ACGTGCCTTG GCTTCGATCG AAATCAACAT GGCTGATATT 
CCAGAAGGAA AAACAAAGAC ATTCGAATGG CGTGGAAAGC CAGTCTTCGT CAAACATCGT 
ACCAAGGCTG AGATTGCCAA GGAAAAGGCC GTCCCTGTCG CCGATCTCCG TCATCCACAA 
CATGACGACG AACGTGTGCA GAAAGATGAA TGGTCCGTTG TCATTGGAGT GTGCACCCAT 
CTTGGATGTG TCCCAATTGC CGATGCTGGA GATTACGGAG GTTATTACTG CCCTTGTCAC 
GGATCTCACT ACGACGCTTC TGGACGTATT AGAAAGGGAC CAGCTCCATT GAATCTTCAC 
GTACCAGCAT ACTCGTTCAA GGATGCCACC ATCGTCATTG GATCGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501361.1
CDS	16..846
Translation	MASLARSGGFAVKVISGSQQHVNFVAATPAADNVAAFRDVPAEFSTAEARQAAVSHSTGGVFTNGLAVKGINVTSRRLAHTDVTFPDMSNYRRDSTKNTQVPARDTEDQRRALPTALYYGAGGVLSLWAGKEVVQTLVSYKAMAADQRALASIEINMADIPEGKTKTFEWRGKPVFVKHRTKAEIAKEKAVPVADLRHPQHDDERVQKDEWSVVIGVCTHLGCVPIADAGDYGGYYCPCHGSHYDASGRIRKGPAPLNLHVPAYSFKDATIVIGSS

Target ORF information:

RefSeq Version	NM_068960.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Cytochrome b-c1 complex subunit Rieske, mitochondrial (isp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068960.4

ORF Insert Sequence:

ATGGCTTCTC TTGCTAGATC TGGAGGTTTT GCAGTCAAAG TGATCAGCGG ATCCCAGCAA 
CATGTAAACT TTGTTGCCGC AACACCAGCC GCCGATAATG TCGCTGCTTT CCGCGATGTT 
CCAGCTGAGT TCTCCACGGC TGAAGCTCGT CAAGCCGCTG TTAGTCACAG CACCGGAGGT 
GTCTTCACCA ATGGACTTGC TGTCAAGGGA ATCAATGTCA CCTCTCGCCG TTTGGCTCAC 
ACTGATGTGA CATTCCCCGA TATGTCAAAC TATCGTCGTG ATTCTACAAA GAACACTCAA 
GTGCCAGCCC GTGACACCGA GGATCAACGT CGTGCTCTTC CAACTGCTCT GTATTATGGA 
GCTGGAGGAG TTCTATCTTT GTGGGCCGGA AAGGAAGTTG TACAGACTCT TGTTTCTTAC 
AAAGCTATGG CAGCTGATCA ACGTGCCTTG GCTTCGATCG AAATCAACAT GGCTGATATT 
CCAGAAGGAA AAACAAAGAC ATTCGAATGG CGTGGAAAGC CAGTCTTCGT CAAACATCGT 
ACCAAGGCTG AGATTGCCAA GGAAAAGGCC GTCCCTGTCG CCGATCTCCG TCATCCACAA 
CATGACGACG AACGTGTGCA GAAAGATGAA TGGTCCGTTG TCATTGGAGT GTGCACCCAT 
CTTGGATGTG TCCCAATTGC CGATGCTGGA GATTACGGAG GTTATTACTG CCCTTGTCAC 
GGATCTCACT ACGACGCTTC TGGACGTATT AGAAAGGGAC CAGCTCCATT GAATCTTCAC 
GTACCAGCAT ACTCGTTCAA GGATGCCACC ATCGTCATTG GATCGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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isp-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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