ORF » Species Summary » Caenorhabditis elegans » hecd-1 cDNA ORF clone

hecd-1 cDNA ORF clone, Caenorhabditis elegans

  • Gene

  • Clones

  • gRNAs

The following hecd-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecd-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa342642 NM_001306759.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342643 NM_001306760.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342644 NM_001306761.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342645 NM_001306762.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342646 NM_001306763.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342647 NM_001306764.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342648 NM_001306765.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OCa342649 NM_001306766.2
Latest version!		 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
View Old Accession Versions >>
OCa342643 NM_001306760.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342645 NM_001306762.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342647 NM_001306764.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342642 NM_001306759.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342644 NM_001306761.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342646 NM_001306763.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342648 NM_001306765.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCa342649 NM_001306766.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    hecd-1 ( NM_001306759.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293688.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342642
    Clone ID Related Accession (Same CDS sequence) NM_001306759.1 , NM_001306759.2
    Accession Version NM_001306759.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT 
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA 
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG 
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA 
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC 
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC 
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT 
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC 
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT 
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT 
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC 
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA 
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT 
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG 
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT 
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT 
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA 
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA 
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT 
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT 
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA 
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT 
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG 
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG 
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA 
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA 
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA 
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG 
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC 
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA 
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT 
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA 
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT 
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT 
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC 
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA 
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG 
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG 
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA 
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG 
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT 
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA 
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA 
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT 
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG 
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC 
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC 
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC 
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC 
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT 
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA 
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA 
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA 
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT 
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA 
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC 
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC 
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT 
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT 
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT 
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT 
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT 
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA 
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA 
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC 
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA 
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC 
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG 
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG 
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC 
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC 
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT 
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC 
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC 
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC 
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC 
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA 
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA 
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG 
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG 
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT 
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT 
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA 
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC 
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT 
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT 
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC 
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293688.1
    CDS1..7947
    Translation

    Target ORF information:

    RefSeq Version NM_001306759.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT 
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA 
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG 
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA 
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC 
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC 
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT 
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC 
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT 
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT 
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC 
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA 
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT 
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG 
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT 
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT 
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA 
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA 
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT 
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT 
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA 
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT 
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG 
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG 
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA 
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA 
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA 
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG 
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC 
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA 
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT 
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA 
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT 
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT 
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC 
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA 
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG 
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG 
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA 
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG 
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT 
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA 
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA 
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT 
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG 
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC 
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC 
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC 
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC 
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT 
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA 
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA 
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA 
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT 
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA 
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC 
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC 
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT 
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT 
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT 
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT 
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT 
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA 
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA 
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC 
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA 
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC 
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG 
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG 
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC 
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC 
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT 
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC 
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC 
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC 
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC 
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA 
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA 
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG 
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG 
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT 
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT 
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA 
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC 
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT 
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT 
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC 
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306759.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293688.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342642
    Clone ID Related Accession (Same CDS sequence) NM_001306759.1 , NM_001306759.2
    Accession Version NM_001306759.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306759.1. 

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    481
    541
    601
    661
    721
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1981
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    6421
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    7381
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    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT 
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA 
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG 
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA 
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC 
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC 
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT 
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC 
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT 
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT 
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC 
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA 
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT 
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG 
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT 
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT 
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA 
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA 
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT 
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT 
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA 
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT 
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG 
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG 
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA 
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA 
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA 
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG 
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC 
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA 
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT 
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA 
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT 
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT 
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC 
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA 
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG 
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG 
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA 
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG 
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT 
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA 
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA 
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT 
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG 
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC 
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC 
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC 
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC 
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT 
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA 
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA 
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA 
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT 
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA 
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC 
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC 
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT 
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT 
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT 
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT 
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT 
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA 
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA 
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC 
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA 
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC 
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG 
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG 
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC 
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC 
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT 
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC 
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC 
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC 
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC 
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA 
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA 
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG 
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG 
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT 
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT 
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA 
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC 
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT 
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT 
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC 
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293688.1
    CDS107..8053
    Translation

    Target ORF information:

    RefSeq Version NM_001306759.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAGACG ATGCTGGTGG TTTCCTTTTC GAAACTGGAA CTGGACGAAA AACGAATGTT 
    ATGCCAGAGC ATGCATTACC ATCGGATTTC CATACTGGAT GGAGTTCACA CGGAGTATCA 
    ACCCGAAAAA TGAAGTTCCG TCAAGATATT CAGAAGCGTA AAGTTCAAGA ATTGGCCTGG 
    AAATTGTGGA ACGATCATCT GAAAGAGGCC CATGCGAAGC CGAGAGAGGC ACTTGTTAGA 
    TTGGAGAATG CAGCACGTAC CATAGAGAGC ACTATTCGAC ACGTTAAAGC TCAGAGCAAC 
    TTCAAACATC GGAATGTGAA GCAACCAAGA ATTGAAAGAG TACAAGAATA TTGTGCTGCC 
    ATCAGTACAC TTCATGAGTC AATCGTTGAT GATCGTCGTT TGTCTACATT CGAATTTTCT 
    GTTTCGGGAA TTGTGCCTGC GCTTTTTGGC CTTTTATCAA TGATGGAAAA GTTTCCAGAC 
    AGTTTCCCAT CAAGGATCTT CAAGGAACAA TTCTCAAAAG GAGAAGCACT TTCATATCTT 
    GCACTGAAAA TAGTTGCTGT TTTGGAAGCG AATGAAAAGT TCCCACAGCA TTTATACGAT 
    AGTCCTGGAG GTTCTTCCTT CGGCCTTCAA CTTCTGTCTC GTCGTGTGCG CACAAAACTC 
    GAAATGCTTC CACGTGCTGA TGGAAAGGAG AATAACGACG AAAATCTTGT GAACAAGACA 
    GGAAAGATAG TAAAGTGCGA ACCTCTTGCA TCTGTGGGAG CCATCAGAGC GTATCTTCAT 
    AGAATGGTTA CTCGACAGTG GCATGATCGT GAACGAGCCA ATTATAGATA TGTCAAAGAG 
    ATTCAAGAGT TGAAGACAAA AGGAAAATCT ATTGAATTGA GACATGTTTC TGACTTTGAT 
    GAGAATGGTG TTATTTATTG GATCGGAACA AATGGAAGAG CTGCACCACT ATGGACAAAT 
    CCAGCAACCG TAAAAGCAGT TAAAATCACT TGTTCGGATA CTCGCCAACC ATTCGGTAAA 
    CCTGAAGATC TTCTGTCACG TGATCAAAAT CCTATCAATT GTCATACTTC CGATGATAAA 
    AATGCTCATT TCACAATTGA TTTGGGTCTG TTCGTTGTTC CAACTTCTTA TTCTCTTCGT 
    CACTCGCGTG GATATGGAAG ATCTGCTCTG CGTAATTGGA TGCTTCAAGG ATCTGTTGAT 
    GCAAAGCGAT GGGAAAATGT GATTGTTCAC ACTGACGATA AAGGACTCGG AGAACCAGGA 
    TCTACTGCTA CTTGGCATGT TGGTGAAAAG GGAACAACCG CATTCAGATT CTTCAGAATT 
    GCTCAAAACG GAAAGAACTC TTCGGGTCAA ACACATTACT TGTCTTGTTC CGGATTCGAG 
    ATTTACGGAG ACATCGTTGA TGTGGTAACA GAAGCCATTT GCGAAGACCC ACCGAAAAAG 
    GATTCACCAG CCGGTACGTC GTCAACTCCT GGATCCTCAT CATCAGCCGC ATTACCTCCA 
    CTCACAAAAG AACAAGTGCT TGAAATGCTT CCAGCTCGTG AGAACAACAA TCGTCTTAAA 
    TCAGGACTAT CGCTGGAGAC TGTCACTTCA ATGCTGCAGA GGTCCCGTCA TCGTTCAAGA 
    GGATCGTACA AAATAAGCGA GTCGAAGTCA AAAGTTGTAC GTGGAAAAGA CTGGAGATGG 
    GAAGATCAGG ATGGCGGAGA AGGAAAATTC GGTCGTATCA CTAGTCCGCC AGAAAGTGGC 
    TGGGTTGATG TGACATGGGA CAATGGAAAC GCCAATTCAT ATCGATTCGG AGCCAATGGA 
    AACTTCGACA TTGAGCGCGT TACCAGCACT GGACATCGTT ATTCAACACC ATCGCTAGCT 
    TCACATGTTC CAACTTCGGT TATGGAGGCA GTTCGACGAA ATCGTGCTTT CTACACTCCA 
    AAAACAACTG GCGGTCCACC ATCATCATCC GTTTTTGGCA CTTCATCATC AGCTGGATCT 
    TCTCGTGGAG CTGCTTCTGC TCTCTCGCGA TTTGCATCAG TGAAAAACAC TACTCCAGCT 
    GGAACTCCAT CATCGGGAGG ATCGTCTGGA GGTGCAATCG GAAAGAAATC AATGTCAACC 
    ACAAATCTCG TCGATGAGCG ACAGAAGACA TCTGGACCAT CCGTTGCTTC AACAGGTCAA 
    GCAGCTTCTG CTGAATCTCT TCAACATCAA ACTCCTTCTC TGGAGAATCT TCTTGCAAGG 
    GCCATGCCAC ACGCATTTGG ACGTATCGCA GAGAATCAAG AACCCGAAGA TGAACCAATG 
    GGTGGAGAAG AGAGTGATTC GGCTGCAAGC ATGAGATCAG CAGCATCGTC AAATTCACAA 
    ATGTCTATGG GATCATCTTC TCAACAACAA CAACAACAAG ATTCAGACAT GACTCCAAGG 
    GACTCTGCGG GAACTCCTTC TACTCCTCGT GATGATAAGA ACCAAACACT CTCCGTTTCT 
    GCACCTGATC TTGCTGCAGC ACGGCAACGA CAAGCTTCAG CCGAAACAGA CGGAGACGCA 
    GATGCGGATG AGACAAACTC TGAAGATAAA ACTGTTGGTG CTGATGATGC AATGGAAGAA 
    GACGACGAAG AGGAGGAAAC AATGGAGGAC GAGGAAGATG ATGATGACGA CGATGATGAT 
    GAAAGCTCCA ACGAAAACCA AGAAAAGCTA GTCGAATTGC TAGGTGGAGA ACGTGGCTTG 
    TTTGATAAAC TTAAAGAAGT CATCACCGGA GAATCATTAT CGGACGCTTC GTCGTCTGCC 
    AAAGACGCTA CCACCAATGA AGCTCAGAAG AAGGGAGGCA AAAAACCTAA AAAGTGGTTC 
    AAAAAGATGT CCAGCTACAC GGATGTTCTT AAAGGTTTAA TGCAAAATCG TTATCCTGTC 
    GCTCTTCTCG ATCCAGCTGC TGCCGGTATT GAAATGGATG AAATGATGGA TGATGATGAC 
    TACTATGATT TCTCGGAAGA TGGTCCAGAT GATGGTGATT CAGTTGAAGA TGAAGTTGCT 
    GCTCACCTTG GAATGCCTGT TGACTCATTT GCATCAATGG TTGCTGCCAG AACCCCAATA 
    ACATGGCGTC AATTTTCTGA ATTAATGAGT GGTTCTAATC GCGAACGTGC TGCTATGGCA 
    AGAGCTGTAG CATCGAGTCG AGGATCCCCA TGGGAAGATG ATGCAACTGT CAAGTGTACA 
    TTCGAAGCCC TTATTCCAGC ATTCGATCCA CGCCCAGGTC GTTCAAATGT GAATCAAACT 
    TTAGAAGTCG AACTTCCAAC CGTCGTAAAA GATTTCGGTT CTACCAAGGC TTCTTCTTCA 
    AAGATTGACA AAGATGATCA AATGCGCTTC TTCCTTCGTG GACCAAACAT GTCAGGTGTC 
    GATAATGTGA CCATCGAGAT GAATGATGAT TCAGCATCCC TGTTCCGATA CATGCAAATC 
    ATTAACAACA GTGTCAATTG GGCTACAAAA AGTGATCGAT CTCGCCGGAT CTGGGAGCCT 
    ACGTACTCAA TCTGTTACTG CTCTGCCGAT CAAACAAATG TCGAAGTCTC CAAAATTCCT 
    GATGAGGAAA GTAGTACACC ATGTCAAGTA AATCAATGTC TTGAAACCAT CGGATTGCTT 
    TCAAGAATTC AACAGGCAAT GCCAGAAGCA GAGATAACTC CAAATGTGTT TATCAGTGAT 
    AAACTGACGT TGAAAGTAAC TCAAGTTCTC TCTGATGCTC TCGTTGTCGC AGCACGTTCT 
    CTTCCTGAAT GGTGCTCTCG TTTAGTCTAT AAATATCCAT GTCTGTTCAC TGTGGAGACA 
    AGAAACATGT ACATGCAGGC TACTGCATTC GGCGTGTCTC GTACAATCGT GTGGCTCCAA 
    CAACGTCGTG ATGCTGCTGT CGAACGTGCC CGCGGAAGTG CTCAAGCTGG AAACAGTAGC 
    GCTGCCCGGC AACATGATCG TTATCACGAA TATCGGGTAG GGCGTCTTCG CCATGAACGA 
    GTTAAAGTGA CTCGAGCTGA AGAGACGCTT CTCGATCAAG CGATCCGATT AATGAAGTTC 
    CATGCTGATC GTAAGGCGGT GCTAGAGATC GAATACACCA ATGAAGAAGG AACAGGATTG 
    GGACCAACTC TTGAGTTCTA TGCATTGGTT GCTGCAGAGT TGCAACGAAA GTCATTGGCG 
    TTGTGGGTTT GTGATGATGA TGATACACAT GCATCAAAGA GTGGAGAAGA AAGAGAAGTC 
    GATCTCGGTG AAGGAAAGAA GCCAATTGGA TACTACGTTC GTCGCGTTGG TGGTCTCTTC 
    CCAGCACCAC TTCCACCAGG AACCGATGAA ACAAAGAGAG CAGCAGATAT GTTCCGTGTT 
    CTCGGAGTAT TTCTTGCTAA AGTTCTTCTC GATGGTCGTC TTGTTGATCT ACCACTATCC 
    CGTCCATTCC TCAAGTTACT CGTCCATCCT CAGATTGGAG ATGATGCTCG TGGACCAAAC 
    TTGCACAAGA TTCTCAGCCT AGACGATTTC GAAGAAGTGA ATCCAGTAAA AGGAAGTTTC 
    CTTAAAGAGT TGCGAGCTCT TGCTCAGCGT AAGCGACTGA TCGAGAACGA CACGAGCATC 
    GATTCGAATT CCAAGAGAAG AAAAATTGCG GAGCTCAAGT TACACATTAA GGGATCCACA 
    TGCAGAGTTG AAGATCTTGC ACTTAATTTC ACTGTCAATC CGCCATCAAA AGTGTTCCAA 
    TATGCCGAAA TGGAGTTGGT TGACGGTGGA TCCGACATCG ATGTTACAAT CGACAACGTG 
    GAGCAATATG TGGAGAAATG TGAAGAATTC TATTTGAACA CAGGAATAGC CTACCAGATG 
    CGTGCTTTCC GTGATGGATT CGATCGCGTT TTTCCACTTA GAACTCTCAG AGCATACTCT 
    CCAGAAGAAG TTCAGCGTCT TTTGTCCGGT GAACAGTGCC CAGAATGGTC TCGGGACGAT 
    ATTCTAAATT ATACGGAGCC AAAACTTGGA TACACTCGAG AATCTCCTGG TTTCTTGAGA 
    TTTGTCGATG TAATGGAAGC ATTGACAGCT CAAGAACGAA AGAATTTCCT TCAATTCGCC 
    ACAGGATGTT CGAGCTTGCC ACCAGGTGGT CTTGCTAATC TTCATCCAAG GTTGACAATT 
    GTGCGAAAAG TGGAAAGTGG AGACGGCAGC TATCCATCGG TTAATACATG TGTGCATTAT 
    CTCAAACTCC CGGAGTACTC CTCGTCTGCA ATCCTCCGCG AACGGCTTCT CACGGCAATC 
    AACGAGAAAG GATTCCATTT GAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306760.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293689.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342643
    Clone ID Related Accession (Same CDS sequence) NM_001306760.1 , NM_001306760.2
    Accession Version NM_001306760.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA 
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC 
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA 
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA 
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT 
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT 
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC 
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG 
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT 
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT 
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC 
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG 
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG 
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT 
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT 
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA 
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA 
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC 
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT 
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA 
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA 
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC 
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC 
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA 
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA 
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT 
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT 
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC 
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA 
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA 
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA 
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC 
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA 
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT 
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA 
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA 
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT 
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT 
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA 
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG 
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC 
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC 
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA 
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC 
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA 
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG 
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA 
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT 
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT 
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT 
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA 
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT 
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC 
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG 
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT 
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG 
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC 
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC 
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC 
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC 
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT 
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA 
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT 
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG 
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA 
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC 
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA 
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA 
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG 
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA 
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT 
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG 
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA 
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT 
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA 
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC 
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG 
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA 
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC 
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC 
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA 
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT 
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT 
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT 
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG 
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT 
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC 
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293689.1
    CDS1..7836
    Translation

    Target ORF information:

    RefSeq Version NM_001306760.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA 
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC 
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA 
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA 
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT 
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT 
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC 
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG 
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT 
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT 
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC 
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG 
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG 
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT 
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT 
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA 
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA 
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC 
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT 
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA 
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA 
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC 
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC 
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA 
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA 
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT 
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT 
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC 
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA 
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA 
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA 
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC 
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA 
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT 
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA 
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA 
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT 
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT 
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA 
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG 
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC 
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC 
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA 
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC 
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA 
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG 
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA 
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT 
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT 
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT 
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA 
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT 
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC 
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG 
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT 
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG 
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC 
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC 
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC 
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC 
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT 
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA 
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT 
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG 
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA 
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC 
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA 
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA 
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG 
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA 
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT 
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG 
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA 
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT 
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA 
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC 
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG 
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA 
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC 
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC 
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA 
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT 
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT 
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT 
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG 
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT 
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC 
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306760.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293689.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342643
    Clone ID Related Accession (Same CDS sequence) NM_001306760.1 , NM_001306760.2
    Accession Version NM_001306760.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306760.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA 
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC 
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA 
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA 
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT 
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT 
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC 
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG 
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT 
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT 
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC 
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG 
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG 
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT 
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT 
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA 
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA 
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC 
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT 
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA 
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA 
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC 
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC 
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA 
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA 
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT 
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT 
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC 
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA 
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA 
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA 
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC 
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA 
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT 
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA 
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA 
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT 
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT 
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA 
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG 
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC 
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC 
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA 
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC 
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA 
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG 
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA 
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT 
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT 
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT 
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA 
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT 
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC 
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG 
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT 
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG 
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC 
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC 
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC 
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC 
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT 
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA 
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT 
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG 
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA 
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC 
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA 
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA 
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG 
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA 
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT 
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG 
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA 
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT 
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA 
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC 
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG 
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA 
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC 
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC 
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA 
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT 
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT 
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT 
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG 
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT 
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC 
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293689.1
    CDS107..7942
    Translation

    Target ORF information:

    RefSeq Version NM_001306760.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306760.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAGACGA TGCTGGTGGT TTCCTTTTCG AAACTGGAAC TGGACGAAAA 
    ACGAATGTTA TGCCAGAGCA TGCATTACCA TCGGATTTCC ATACTGGATG GAGTTCACAC 
    GGAGTATCAA CCCGAAAAAT GAAGTTCCGT CAAGATATTC AGAAGCGTAA AGTTCAAGAA 
    TTGGCCTGGA AATTGTGGAA CGATCATCTG AAAGAGGCCC ATGCGAAGCC GAGAGAGGCA 
    CTTGTTAGAT TGGAGAATGC AGCACGTACC ATAGAGAGCA CTATTCGACA CGTTAAAGCT 
    CAGAGCAACT TCAAACATCG GAATGTGAAG CAACCAAGAA TTGAAAGAGT ACAAGAATAT 
    TGTGCTGCCA TCAGTACACT TCATGAGTCA ATCGTTGATG ATCGTCGTTT GTCTACATTC 
    GAATTTTCTG TTTCGGGAAT TGTGCCTGCG CTTTTTGGCC TTTTATCAAT GATGGAAAAG 
    TTTCCAGACA GTTTCCCATC AAGGATCTTC AAGGAACAAT TCTCAAAAGG AGAAGCACTT 
    TCATATCTTG CACTGAAAAT AGTTGCTGTT TTGGAAGCGA ATGAAAAGTT CCCACAGCAT 
    TTATACGATA GTCCTGGAGG TTCTTCCTTC GGCCTTCAAC TTCTGTCTCG TCGTGTGCGC 
    ACAAAACTCG AAATGCTTCC ACGTGCTGAT GGAAAGGAGA ATAACGACGA AAATCTTGTG 
    AACAAGACAG GAAAGATAGT AAAGTGCGAA CCTCTTGCAT CTGTGGGAGC CATCAGAGCG 
    TATCTTCATA GAATGGTTAC TCGACAGTGG CATGATCGTG AACGAGCCAA TTATAGATAT 
    GTCAAAGAGA TTCAAGAGTT GAAGACAAAA GGAAAATCTA TTGAATTGAG ACATGTTTCT 
    GACTTTGATG AGAATGGTGT TATTTATTGG ATCGGAACAA ATGGAAGAGC TGCACCACTA 
    TGGACAAATC CAGCAACCGT AAAAGCAGTT AAAATCACTT GTTCGGATAC TCGCCAACCA 
    TTCGGTAAAC CTGAAGATCT TCTGTCACGT GATCAAAATC CTATCAATTG TCATACTTCC 
    GATGATAAAA ATGCTCATTT CACAATTGAT TTGGGTCTGT TCGTTGTTCC AACTTCTTAT 
    TCTCTTCGTC ACTCGCGTGG ATATGGAAGA TCTGCTCTGC GTAATTGGAT GCTTCAAGGA 
    TCTGTTGATG CAAAGCGATG GGAAAATGTG ATTGTTCACA CTGACGATAA AGGACTCGGA 
    GAACCAGGAT CTACTGCTAC TTGGCATGTT GGTGAAAAGG GAACAACCGC ATTCAGATTC 
    TTCAGAATTG CTCAAAACGG AAAGAACTCT TCGGGTCAAA CACATTACTT GTCTTGTTCC 
    GGATTCGAGA TTTACGGAGA CATCGTTGAT GTGGTAACAG AAGCCATTTG CGAAGACCCA 
    CCGAAAAAGG ATTCACCAGC CGGTACGTCG TCAACTCCTG GATCCTCATC ATCAGCCGCA 
    TTACCTCCAC TCACAAAAGA ACAAGTGCTT GAAATGCTTC CAGCTCGTGA GAACAACAAT 
    CGTCTTAAAT CAGGACTATC GCTGGAGACT GTCACTTCAA TGCTGCAGAG GTCCCGTCAT 
    CGTTCAAGAG GATCGTACAA AATAAGCGAG TCGAAGTCAA AAGTTGTACG TGGAAAAGAC 
    TGGAGATGGG AAGATCAGGA TGGCGGAGAA GGAAAATTCG GTCGTATCAC TAGTCCGCCA 
    GAAAGTGGCT GGGTTGATGT GACATGGGAC AATGGAAACG CCAATTCATA TCGATTCGGA 
    GCCAATGGAA ACTTCGACAT TGAGCGCGTT ACCAGCACTG GACATCGTTA TTCAACACCA 
    TCGCTAGCTT CACATGTTCC AACTTCGGTT ATGGAGGCAG TTCGACGAAA TCGTGCTTTC 
    TACACTCCAA AAACAACTGG CGGTCCACCA TCATCATCCG TTTTTGGCAC TTCATCATCA 
    GCTGGATCTT CTCGTGGAGC TGCTTCTGCT CTCTCGCGAT TTGCATCAGT GAAAAACACT 
    ACTCCAGCTG GAACTCCATC ATCGGGAGGA TCGTCTGGAG GTGCAATCGG AAAGAAATCA 
    ATGTCAACCA CAAATCTCGT CGATGAGCGA CAGAAGACAT CTGGACCATC CGTTGCTTCA 
    ACAGGTCAAG CAGCTTCTGC TGAATCTCTT CAACATCAAA CTCCTTCTCT GGAGAATCTT 
    CTTGCAAGGG CCATGCCACA CGCATTTGGA CGTATCGCAG AGAATCAAGA ACCCGAAGAT 
    GAACCAATGG GTGGAGAAGA GAGTGATTCG GCTGCAAGCA TGAGATCAGC AGCATCGTCA 
    AATTCACAAA TGTCTATGGG ATCATCTTCT CAACAACAAC AACAACAAGA TTCAGACATG 
    ACTCCAAGGG ACTCTGCGGG AACTCCTTCT ACTCCTCGTG ATGATAAGAA CCAAACACTC 
    TCCGTTTCTG CACCTGATCT TGCTGCAGCA CGGCAACGAC AAGCTTCAGC CGAAACAGAC 
    GGAGACGCAG ATGCGGATGA GACAAACTCT GAAGATAAAA CTGTTGGTGC TGATGATGCA 
    ATGGAAGAAG ACGACGAAGA GGAGGAAACA ATGGAGGACG AGGAAGATGA TGATGACGAC 
    GATGATGATG AAAGCTCCAA CGAAAACCAA GAAAAGCTAG TCGAATTGCT AGGTGGAGAA 
    CGTGGCTTGT TTGATAAACT TAAAGAAGTC ATCACCGGAG AATCATTATC GGACGCTTCG 
    TCGTCTGCCA AAGACGCTAC CACCAATGAA GCTCAGAAGA AGGGAGGCAA AAAACCTAAA 
    AAGTGGTTCA AAAAGATGTC CAGCTACACG GATGTTCTTA AAGGTTTAAT GCAAAATCGT 
    TATCCTGTCG CTCTTCTCGA TCCAGCTGCT GCCGGTATTG AAATGGATGA AATGATGGAT 
    GATGATGACT ACTATGATTT CTCGGAAGAT GGTCCAGATG ATGGTGATTC AGTTGAAGAT 
    GAAGTTGCTG CTCACCTTGG AATGCCTGTT GACTCATTTG CATCAATGGT TGCTGCCAGA 
    ACCCCAATAA CATGGCGTCA ATTTTCTGAA TTAATGAGTG GTTCTAATCG CGAACGTGCT 
    GCTATGGCAA GAGCTGTAGC ATCGAGTCGA GGATCCCCAT GGGAAGATGA TGCAACTGTC 
    AAGTGTACAT TCGAAGCCCT TATTCCAGCA TTCGATCCAC GCCCAGGTCG TTCAAATGTG 
    AATCAAACTT TAGAAGTCGA ACTTCCAACC GTCGTAAAAG ATTTCGGTTC TACCAAGGCT 
    TCTTCTTCAA AGATTGACAA AGATGATCAA ATGCGCTTCT TCCTTCGTGG ACCAAACATG 
    TCAGGTGTCG ATAATGTGAC CATCGAGATG AATGATGATT CAGCATCCCT GTTCCGATAC 
    ATGCAAATCA TTAACAACAG TGTCAATTGG GCTACAAAAA GTGATCGATC TCGCCGGATC 
    TGGGAGCCTA CGTACTCAAT CTGTTACTGC TCTGCCGATC AAACAAATGT CGAAGTCTCC 
    AAAATTCCTG ATGAGGAAAG TAGTACACCA TGTCAAGTAA ATCAATGTCT TGAAACCATC 
    GGATTGCTTT CAAGAATTCA ACAGGCAATG CCAGAAGCAG AGATAACTCC AAATGTGTTT 
    ATCAGTGATA AACTGACGTT GAAAGTAACT CAAGTTCTCT CTGATGCTCT CGTTGTCGCA 
    GCACGTTCTC TTCCTGAATG GTGCTCTCGT TTAGTCTATA AATATCCATG TCTGTTCACT 
    GTGGAGACAA GAAACATGTA CATGCAGGCT ACTGCATTCG GCGTGTCTCG TACAATCGTG 
    TGGCTCCAAC AACGTCGTGA TGCTGCTGTC GAACGTGCCC GCGGAAGTGC TCAAGCTGGA 
    AACAGTAGCG CTGCCCGGCA ACATGATCGT TATCACGAAT ATCGGGTAGG GCGTCTTCGC 
    CATGAACGAG TTAAAGTGAC TCGAGCTGAA GAGACGCTTC TCGATCAAGC GATCCGATTA 
    ATGAAGTTCC ATGCTGATCG TAAGGCGGTG CTAGAGATCG AATACACCAA TGAAGAAGGA 
    ACAGGATTGG GACCAACTCT TGAGTTCTAT GCATTGGTTG CTGCAGAGTT GCAACGAAAG 
    TCATTGGCGT TGTGGGTTTG TGATGATGAT GATACACATG CATCAAAGAG TGGAGAAGAA 
    AGAGAAGTCG ATCTCGGTGA AGGAAAGAAG CCAATTGGAT ACTACGTTCG TCGCGTTGGT 
    GGTCTCTTCC CAGCACCACT TCCACCAGGA ACCGATGAAA CAAAGAGAGC AGCAGATATG 
    TTCCGTGTTC TCGGAGTATT TCTTGCTAAA GTTCTTCTCG ATGGTCGTCT TGTTGATCTA 
    CCACTATCCC GTCCATTCCT CAAGTTACTC GTCCATCCTC AGATTGGAGA TGATGCTCGT 
    GGACCAAACT TGCACAAGAT TCTCAGCCTA GACGATTTCG AAGAAGTGAA TCCAGTAAAA 
    GGAAGTTTCC TTAAAGAGTT GCGAGCTCTT GCTCAGCGTA AGCGACTGAT CGAGAACGAC 
    ACGAGCATCG ATTCGAATTC CAAGAGAAGA AAAATTGCGG AGCTCAAGTT ACACATTAAG 
    GGATCCACAT GCAGAGTTGA AGATCTTGCA CTTAATTTCA CTGTCAATCC GCCATCAAAA 
    GTGTTCCAAT ATGCCGAAAT GGAGTTGGTT GACGGTGGAT CCGACATCGA TGTTACAATC 
    GACAACGTGG AGCAATATGT GGAGAAATGT GAAGAATTCT ATTTGAACAC AGGAATAGCC 
    TACCAGATGC GTGCTTTCCG TGATGGATTC GATCGCGTTT TTCCACTTAG AACTCTCAGA 
    GCATACTCTC CAGAAGAAGT TCAGCGTCTT TTGTCCGGTG AACAGTGCCC AGAATGGTCT 
    CGGGACGATA TTCTAAATTA TACGGAGCCA AAACTTGGAT ACACTCGAGA ATCTCCTGGT 
    TTCTTGAGAT TTGTCGATGT AATGGAAGCA TTGACAGCTC AAGAACGAAA GAATTTCCTT 
    CAATTCGCCA CAGGATGTTC GAGCTTGCCA CCAGGTGGTC TTGCTAATCT TCATCCAAGG 
    TTGACAATTG TGCGAAAAGT GGAAAGTGGA GACGGCAGCT ATCCATCGGT TAATACATGT 
    GTGCATTATC TCAAACTCCC GGAGTACTCC TCGTCTGCAA TCCTCCGCGA ACGGCTTCTC 
    ACGGCAATCA ACGAGAAAGG ATTCCATTTG AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306761.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293690.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342644
    Clone ID Related Accession (Same CDS sequence) NM_001306761.1 , NM_001306761.2
    Accession Version NM_001306761.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG 
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC 
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA 
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG 
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC 
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC 
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT 
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT 
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA 
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT 
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA 
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA 
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA 
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT 
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG 
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA 
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT 
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT 
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC 
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA 
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT 
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT 
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT 
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT 
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC 
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA 
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA 
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA 
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG 
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC 
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA 
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA 
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT 
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA 
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA 
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA 
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC 
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT 
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG 
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC 
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA 
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT 
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC 
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA 
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT 
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA 
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA 
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT 
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC 
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT 
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA 
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA 
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC 
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA 
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG 
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT 
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT 
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG 
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT 
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA 
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA 
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT 
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA 
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA 
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT 
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT 
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT 
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA 
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG 
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT 
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA 
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC 
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT 
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG 
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT 
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT 
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC 
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT 
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG 
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT 
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA 
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT 
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT 
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA 
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG 
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC 
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC 
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293690.1
    CDS1..7824
    Translation

    Target ORF information:

    RefSeq Version NM_001306761.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG 
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC 
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA 
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG 
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC 
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC 
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT 
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT 
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA 
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT 
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA 
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA 
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA 
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT 
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG 
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA 
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT 
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT 
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC 
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA 
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT 
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT 
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT 
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT 
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC 
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA 
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA 
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA 
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG 
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC 
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA 
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA 
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT 
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA 
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA 
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA 
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC 
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT 
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG 
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC 
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA 
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT 
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC 
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA 
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT 
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA 
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA 
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT 
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC 
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT 
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA 
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA 
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC 
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA 
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG 
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT 
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT 
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG 
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT 
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA 
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA 
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT 
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA 
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA 
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT 
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT 
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT 
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA 
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG 
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT 
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA 
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC 
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT 
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG 
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT 
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT 
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC 
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT 
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG 
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT 
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA 
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT 
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT 
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA 
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG 
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC 
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC 
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306761.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293690.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342644
    Clone ID Related Accession (Same CDS sequence) NM_001306761.1 , NM_001306761.2
    Accession Version NM_001306761.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306761.1. 

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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG 
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC 
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA 
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG 
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC 
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC 
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT 
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT 
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA 
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT 
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA 
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA 
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA 
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT 
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG 
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA 
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT 
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT 
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC 
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA 
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT 
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT 
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT 
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT 
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC 
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA 
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA 
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA 
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG 
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC 
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA 
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA 
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT 
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA 
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA 
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA 
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC 
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT 
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG 
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC 
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA 
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT 
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC 
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA 
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT 
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA 
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA 
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT 
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC 
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT 
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA 
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA 
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC 
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA 
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG 
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT 
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT 
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG 
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT 
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA 
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA 
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT 
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA 
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA 
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT 
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT 
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT 
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA 
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG 
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT 
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA 
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC 
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT 
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG 
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT 
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT 
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC 
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT 
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG 
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT 
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA 
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT 
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT 
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA 
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG 
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC 
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC 
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293690.1
    CDS242..8065
    Translation

    Target ORF information:

    RefSeq Version NM_001306761.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306761.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAGACGATG CTGGTGGTTT CCTTTTCGAA ACTGGAACTG GACGAAAAAC GAATGTTATG 
    CCAGAGCATG CATTACCATC GGATTTCCAT ACTGGATGGA GTTCACACGG AGTATCAACC 
    CGAAAAATGA AGTTCCGTCA AGATATTCAG AAGCGTAAAG TTCAAGAATT GGCCTGGAAA 
    TTGTGGAACG ATCATCTGAA AGAGGCCCAT GCGAAGCCGA GAGAGGCACT TGTTAGATTG 
    GAGAATGCAG CACGTACCAT AGAGAGCACT ATTCGACACG TTAAAGCTCA GAGCAACTTC 
    AAACATCGGA ATGTGAAGCA ACCAAGAATT GAAAGAGTAC AAGAATATTG TGCTGCCATC 
    AGTACACTTC ATGAGTCAAT CGTTGATGAT CGTCGTTTGT CTACATTCGA ATTTTCTGTT 
    TCGGGAATTG TGCCTGCGCT TTTTGGCCTT TTATCAATGA TGGAAAAGTT TCCAGACAGT 
    TTCCCATCAA GGATCTTCAA GGAACAATTC TCAAAAGGAG AAGCACTTTC ATATCTTGCA 
    CTGAAAATAG TTGCTGTTTT GGAAGCGAAT GAAAAGTTCC CACAGCATTT ATACGATAGT 
    CCTGGAGGTT CTTCCTTCGG CCTTCAACTT CTGTCTCGTC GTGTGCGCAC AAAACTCGAA 
    ATGCTTCCAC GTGCTGATGG AAAGGAGAAT AACGACGAAA ATCTTGTGAA CAAGACAGGA 
    AAGATAGTAA AGTGCGAACC TCTTGCATCT GTGGGAGCCA TCAGAGCGTA TCTTCATAGA 
    ATGGTTACTC GACAGTGGCA TGATCGTGAA CGAGCCAATT ATAGATATGT CAAAGAGATT 
    CAAGAGTTGA AGACAAAAGG AAAATCTATT GAATTGAGAC ATGTTTCTGA CTTTGATGAG 
    AATGGTGTTA TTTATTGGAT CGGAACAAAT GGAAGAGCTG CACCACTATG GACAAATCCA 
    GCAACCGTAA AAGCAGTTAA AATCACTTGT TCGGATACTC GCCAACCATT CGGTAAACCT 
    GAAGATCTTC TGTCACGTGA TCAAAATCCT ATCAATTGTC ATACTTCCGA TGATAAAAAT 
    GCTCATTTCA CAATTGATTT GGGTCTGTTC GTTGTTCCAA CTTCTTATTC TCTTCGTCAC 
    TCGCGTGGAT ATGGAAGATC TGCTCTGCGT AATTGGATGC TTCAAGGATC TGTTGATGCA 
    AAGCGATGGG AAAATGTGAT TGTTCACACT GACGATAAAG GACTCGGAGA ACCAGGATCT 
    ACTGCTACTT GGCATGTTGG TGAAAAGGGA ACAACCGCAT TCAGATTCTT CAGAATTGCT 
    CAAAACGGAA AGAACTCTTC GGGTCAAACA CATTACTTGT CTTGTTCCGG ATTCGAGATT 
    TACGGAGACA TCGTTGATGT GGTAACAGAA GCCATTTGCG AAGACCCACC GAAAAAGGAT 
    TCACCAGCCG GTACGTCGTC AACTCCTGGA TCCTCATCAT CAGCCGCATT ACCTCCACTC 
    ACAAAAGAAC AAGTGCTTGA AATGCTTCCA GCTCGTGAGA ACAACAATCG TCTTAAATCA 
    GGACTATCGC TGGAGACTGT CACTTCAATG CTGCAGAGGT CCCGTCATCG TTCAAGAGGA 
    TCGTACAAAA TAAGCGAGTC GAAGTCAAAA GTTGTACGTG GAAAAGACTG GAGATGGGAA 
    GATCAGGATG GCGGAGAAGG AAAATTCGGT CGTATCACTA GTCCGCCAGA AAGTGGCTGG 
    GTTGATGTGA CATGGGACAA TGGAAACGCC AATTCATATC GATTCGGAGC CAATGGAAAC 
    TTCGACATTG AGCGCGTTAC CAGCACTGGA CATCGTTATT CAACACCATC GCTAGCTTCA 
    CATGTTCCAA CTTCGGTTAT GGAGGCAGTT CGACGAAATC GTGCTTTCTA CACTCCAAAA 
    ACAACTGGCG GTCCACCATC ATCATCCGTT TTTGGCACTT CATCATCAGC TGGATCTTCT 
    CGTGGAGCTG CTTCTGCTCT CTCGCGATTT GCATCAGTGA AAAACACTAC TCCAGCTGGA 
    ACTCCATCAT CGGGAGGATC GTCTGGAGGT GCAATCGGAA AGAAATCAAT GTCAACCACA 
    AATCTCGTCG ATGAGCGACA GAAGACATCT GGACCATCCG TTGCTTCAAC AGGTCAAGCA 
    GCTTCTGCTG AATCTCTTCA ACATCAAACT CCTTCTCTGG AGAATCTTCT TGCAAGGGCC 
    ATGCCACACG CATTTGGACG TATCGCAGAG AATCAAGAAC CCGAAGATGA ACCAATGGGT 
    GGAGAAGAGA GTGATTCGGC TGCAAGCATG AGATCAGCAG CATCGTCAAA TTCACAAATG 
    TCTATGGGAT CATCTTCTCA ACAACAACAA CAACAAGATT CAGACATGAC TCCAAGGGAC 
    TCTGCGGGAA CTCCTTCTAC TCCTCGTGAT GATAAGAACC AAACACTCTC CGTTTCTGCA 
    CCTGATCTTG CTGCAGCACG GCAACGACAA GCTTCAGCCG AAACAGACGG AGACGCAGAT 
    GCGGATGAGA CAAACTCTGA AGATAAAACT GTTGGTGCTG ATGATGCAAT GGAAGAAGAC 
    GACGAAGAGG AGGAAACAAT GGAGGACGAG GAAGATGATG ATGACGACGA TGATGATGAA 
    AGCTCCAACG AAAACCAAGA AAAGCTAGTC GAATTGCTAG GTGGAGAACG TGGCTTGTTT 
    GATAAACTTA AAGAAGTCAT CACCGGAGAA TCATTATCGG ACGCTTCGTC GTCTGCCAAA 
    GACGCTACCA CCAATGAAGC TCAGAAGAAG GGAGGCAAAA AACCTAAAAA GTGGTTCAAA 
    AAGATGTCCA GCTACACGGA TGTTCTTAAA GGTTTAATGC AAAATCGTTA TCCTGTCGCT 
    CTTCTCGATC CAGCTGCTGC CGGTATTGAA ATGGATGAAA TGATGGATGA TGATGACTAC 
    TATGATTTCT CGGAAGATGG TCCAGATGAT GGTGATTCAG TTGAAGATGA AGTTGCTGCT 
    CACCTTGGAA TGCCTGTTGA CTCATTTGCA TCAATGGTTG CTGCCAGAAC CCCAATAACA 
    TGGCGTCAAT TTTCTGAATT AATGAGTGGT TCTAATCGCG AACGTGCTGC TATGGCAAGA 
    GCTGTAGCAT CGAGTCGAGG ATCCCCATGG GAAGATGATG CAACTGTCAA GTGTACATTC 
    GAAGCCCTTA TTCCAGCATT CGATCCACGC CCAGGTCGTT CAAATGTGAA TCAAACTTTA 
    GAAGTCGAAC TTCCAACCGT CGTAAAAGAT TTCGGTTCTA CCAAGGCTTC TTCTTCAAAG 
    ATTGACAAAG ATGATCAAAT GCGCTTCTTC CTTCGTGGAC CAAACATGTC AGGTGTCGAT 
    AATGTGACCA TCGAGATGAA TGATGATTCA GCATCCCTGT TCCGATACAT GCAAATCATT 
    AACAACAGTG TCAATTGGGC TACAAAAAGT GATCGATCTC GCCGGATCTG GGAGCCTACG 
    TACTCAATCT GTTACTGCTC TGCCGATCAA ACAAATGTCG AAGTCTCCAA AATTCCTGAT 
    GAGGAAAGTA GTACACCATG TCAAGTAAAT CAATGTCTTG AAACCATCGG ATTGCTTTCA 
    AGAATTCAAC AGGCAATGCC AGAAGCAGAG ATAACTCCAA ATGTGTTTAT CAGTGATAAA 
    CTGACGTTGA AAGTAACTCA AGTTCTCTCT GATGCTCTCG TTGTCGCAGC ACGTTCTCTT 
    CCTGAATGGT GCTCTCGTTT AGTCTATAAA TATCCATGTC TGTTCACTGT GGAGACAAGA 
    AACATGTACA TGCAGGCTAC TGCATTCGGC GTGTCTCGTA CAATCGTGTG GCTCCAACAA 
    CGTCGTGATG CTGCTGTCGA ACGTGCCCGC GGAAGTGCTC AAGCTGGAAA CAGTAGCGCT 
    GCCCGGCAAC ATGATCGTTA TCACGAATAT CGGGTAGGGC GTCTTCGCCA TGAACGAGTT 
    AAAGTGACTC GAGCTGAAGA GACGCTTCTC GATCAAGCGA TCCGATTAAT GAAGTTCCAT 
    GCTGATCGTA AGGCGGTGCT AGAGATCGAA TACACCAATG AAGAAGGAAC AGGATTGGGA 
    CCAACTCTTG AGTTCTATGC ATTGGTTGCT GCAGAGTTGC AACGAAAGTC ATTGGCGTTG 
    TGGGTTTGTG ATGATGATGA TACACATGCA TCAAAGAGTG GAGAAGAAAG AGAAGTCGAT 
    CTCGGTGAAG GAAAGAAGCC AATTGGATAC TACGTTCGTC GCGTTGGTGG TCTCTTCCCA 
    GCACCACTTC CACCAGGAAC CGATGAAACA AAGAGAGCAG CAGATATGTT CCGTGTTCTC 
    GGAGTATTTC TTGCTAAAGT TCTTCTCGAT GGTCGTCTTG TTGATCTACC ACTATCCCGT 
    CCATTCCTCA AGTTACTCGT CCATCCTCAG ATTGGAGATG ATGCTCGTGG ACCAAACTTG 
    CACAAGATTC TCAGCCTAGA CGATTTCGAA GAAGTGAATC CAGTAAAAGG AAGTTTCCTT 
    AAAGAGTTGC GAGCTCTTGC TCAGCGTAAG CGACTGATCG AGAACGACAC GAGCATCGAT 
    TCGAATTCCA AGAGAAGAAA AATTGCGGAG CTCAAGTTAC ACATTAAGGG ATCCACATGC 
    AGAGTTGAAG ATCTTGCACT TAATTTCACT GTCAATCCGC CATCAAAAGT GTTCCAATAT 
    GCCGAAATGG AGTTGGTTGA CGGTGGATCC GACATCGATG TTACAATCGA CAACGTGGAG 
    CAATATGTGG AGAAATGTGA AGAATTCTAT TTGAACACAG GAATAGCCTA CCAGATGCGT 
    GCTTTCCGTG ATGGATTCGA TCGCGTTTTT CCACTTAGAA CTCTCAGAGC ATACTCTCCA 
    GAAGAAGTTC AGCGTCTTTT GTCCGGTGAA CAGTGCCCAG AATGGTCTCG GGACGATATT 
    CTAAATTATA CGGAGCCAAA ACTTGGATAC ACTCGAGAAT CTCCTGGTTT CTTGAGATTT 
    GTCGATGTAA TGGAAGCATT GACAGCTCAA GAACGAAAGA ATTTCCTTCA ATTCGCCACA 
    GGATGTTCGA GCTTGCCACC AGGTGGTCTT GCTAATCTTC ATCCAAGGTT GACAATTGTG 
    CGAAAAGTGG AAAGTGGAGA CGGCAGCTAT CCATCGGTTA ATACATGTGT GCATTATCTC 
    AAACTCCCGG AGTACTCCTC GTCTGCAATC CTCCGCGAAC GGCTTCTCAC GGCAATCAAC 
    GAGAAAGGAT TCCATTTGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306762.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293691.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342645
    Clone ID Related Accession (Same CDS sequence) NM_001306762.2 , NM_001306762.1
    Accession Version NM_001306762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306762.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT 
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT 
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG 
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC 
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA 
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG 
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT 
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT 
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA 
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA 
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT 
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA 
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA 
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT 
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG 
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT 
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA 
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT 
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC 
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA 
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG 
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT 
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA 
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC 
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC 
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA 
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG 
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA 
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT 
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG 
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT 
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA 
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC 
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT 
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA 
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC 
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT 
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC 
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG 
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA 
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA 
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT 
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG 
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG 
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC 
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT 
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC 
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC 
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT 
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC 
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA 
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA 
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA 
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT 
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA 
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA 
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC 
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT 
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC 
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT 
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA 
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG 
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG 
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC 
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT 
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA 
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT 
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT 
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT 
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT 
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA 
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT 
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT 
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC 
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT 
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG 
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC 
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA 
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG 
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG 
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT 
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT 
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT 
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA 
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG 
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG 
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG 
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA 
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293691.1
    CDS107..8041
    Translation

    Target ORF information:

    RefSeq Version NM_001306762.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT 
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT 
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG 
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC 
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA 
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG 
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT 
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT 
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA 
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA 
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT 
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA 
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA 
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT 
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG 
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT 
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA 
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT 
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC 
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA 
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG 
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT 
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA 
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC 
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC 
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA 
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG 
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA 
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT 
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG 
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT 
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA 
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC 
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT 
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA 
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC 
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT 
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC 
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG 
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA 
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA 
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT 
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG 
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG 
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC 
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT 
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC 
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC 
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT 
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC 
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA 
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA 
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA 
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT 
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA 
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA 
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC 
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT 
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC 
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT 
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA 
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG 
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG 
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC 
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT 
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA 
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT 
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT 
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT 
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT 
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA 
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT 
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT 
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC 
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT 
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG 
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC 
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA 
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG 
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG 
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT 
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT 
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT 
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA 
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG 
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG 
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG 
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA 
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306762.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293691.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342645
    Clone ID Related Accession (Same CDS sequence) NM_001306762.2 , NM_001306762.1
    Accession Version NM_001306762.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT 
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT 
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG 
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC 
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA 
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG 
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT 
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT 
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA 
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA 
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT 
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA 
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA 
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT 
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG 
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT 
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA 
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT 
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC 
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA 
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG 
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT 
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA 
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC 
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC 
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA 
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG 
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA 
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT 
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG 
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT 
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA 
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC 
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT 
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA 
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC 
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT 
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC 
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG 
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA 
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA 
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT 
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG 
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG 
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC 
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT 
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC 
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC 
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT 
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC 
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA 
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA 
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA 
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT 
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA 
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA 
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC 
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT 
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC 
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT 
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA 
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG 
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG 
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC 
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT 
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA 
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT 
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT 
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT 
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT 
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA 
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT 
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT 
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC 
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT 
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG 
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC 
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA 
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG 
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG 
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT 
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT 
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT 
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA 
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG 
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG 
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG 
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA 
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293691.1
    CDS1..7935
    Translation

    Target ORF information:

    RefSeq Version NM_001306762.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAGACGAT 
    GCTGGTGGTT TCCTTTTCGA AACTGGAACT GGACGAAAAA CGAATGTTAT GCCAGAGCAT 
    GCATTACCAT CGGATTTCCA TACTGGATGG AGTTCACACG GAGTATCAAC CCGAAAAATG 
    AAGTTCCGTC AAGATATTCA GAAGCGTAAA GTTCAAGAAT TGGCCTGGAA ATTGTGGAAC 
    GATCATCTGA AAGAGGCCCA TGCGAAGCCG AGAGAGGCAC TTGTTAGATT GGAGAATGCA 
    GCACGTACCA TAGAGAGCAC TATTCGACAC GTTAAAGCTC AGAGCAACTT CAAACATCGG 
    AATGTGAAGC AACCAAGAAT TGAAAGAGTA CAAGAATATT GTGCTGCCAT CAGTACACTT 
    CATGAGTCAA TCGTTGATGA TCGTCGTTTG TCTACATTCG AATTTTCTGT TTCGGGAATT 
    GTGCCTGCGC TTTTTGGCCT TTTATCAATG ATGGAAAAGT TTCCAGACAG TTTCCCATCA 
    AGGATCTTCA AGGAACAATT CTCAAAAGGA GAAGCACTTT CATATCTTGC ACTGAAAATA 
    GTTGCTGTTT TGGAAGCGAA TGAAAAGTTC CCACAGCATT TATACGATAG TCCTGGAGGT 
    TCTTCCTTCG GCCTTCAACT TCTGTCTCGT CGTGTGCGCA CAAAACTCGA AATGCTTCCA 
    CGTGCTGATG GAAAGGAGAA TAACGACGAA AATCTTGTGA ACAAGACAGG AAAGATAGTA 
    AAGTGCGAAC CTCTTGCATC TGTGGGAGCC ATCAGAGCGT ATCTTCATAG AATGGTTACT 
    CGACAGTGGC ATGATCGTGA ACGAGCCAAT TATAGATATG TCAAAGAGAT TCAAGAGTTG 
    AAGACAAAAG GAAAATCTAT TGAATTGAGA CATGTTTCTG ACTTTGATGA GAATGGTGTT 
    ATTTATTGGA TCGGAACAAA TGGAAGAGCT GCACCACTAT GGACAAATCC AGCAACCGTA 
    AAAGCAGTTA AAATCACTTG TTCGGATACT CGCCAACCAT TCGGTAAACC TGAAGATCTT 
    CTGTCACGTG ATCAAAATCC TATCAATTGT CATACTTCCG ATGATAAAAA TGCTCATTTC 
    ACAATTGATT TGGGTCTGTT CGTTGTTCCA ACTTCTTATT CTCTTCGTCA CTCGCGTGGA 
    TATGGAAGAT CTGCTCTGCG TAATTGGATG CTTCAAGGAT CTGTTGATGC AAAGCGATGG 
    GAAAATGTGA TTGTTCACAC TGACGATAAA GGACTCGGAG AACCAGGATC TACTGCTACT 
    TGGCATGTTG GTGAAAAGGG AACAACCGCA TTCAGATTCT TCAGAATTGC TCAAAACGGA 
    AAGAACTCTT CGGGTCAAAC ACATTACTTG TCTTGTTCCG GATTCGAGAT TTACGGAGAC 
    ATCGTTGATG TGGTAACAGA AGCCATTTGC GAAGACCCAC CGAAAAAGGA TTCACCAGCC 
    GGTACGTCGT CAACTCCTGG ATCCTCATCA TCAGCCGCAT TACCTCCACT CACAAAAGAA 
    CAAGTGCTTG AAATGCTTCC AGCTCGTGAG AACAACAATC GTCTTAAATC AGGACTATCG 
    CTGGAGACTG TCACTTCAAT GCTGCAGAGG TCCCGTCATC GTTCAAGAGG ATCGTACAAA 
    ATAAGCGAGT CGAAGTCAAA AGTTGTACGT GGAAAAGACT GGAGATGGGA AGATCAGGAT 
    GGCGGAGAAG GAAAATTCGG TCGTATCACT AGTCCGCCAG AAAGTGGCTG GGTTGATGTG 
    ACATGGGACA ATGGAAACGC CAATTCATAT CGATTCGGAG CCAATGGAAA CTTCGACATT 
    GAGCGCGTTA CCAGCACTGG ACATCGTTAT TCAACACCAT CGCTAGCTTC ACATGTTCCA 
    ACTTCGGTTA TGGAGGCAGT TCGACGAAAT CGTGCTTTCT ACACTCCAAA AACAACTGGC 
    GGTCCACCAT CATCATCCGT TTTTGGCACT TCATCATCAG CTGGATCTTC TCGTGGAGCT 
    GCTTCTGCTC TCTCGCGATT TGCATCAGTG AAAAACACTA CTCCAGCTGG AACTCCATCA 
    TCGGGAGGAT CGTCTGGAGG TGCAATCGGA AAGAAATCAA TGTCAACCAC AAATCTCGTC 
    GATGAGCGAC AGAAGACATC TGGACCATCC GTTGCTTCAA CAGGTCAAGC AGCTTCTGCT 
    GAATCTCTTC AACATCAAAC TCCTTCTCTG GAGAATCTTC TTGCAAGGGC CATGCCACAC 
    GCATTTGGAC GTATCGCAGA GAATCAAGAA CCCGAAGATG AACCAATGGG TGGAGAAGAG 
    AGTGATTCGG CTGCAAGCAT GAGATCAGCA GCATCGTCAA ATTCACAAAT GTCTATGGGA 
    TCATCTTCTC AACAACAACA ACAACAAGAT TCAGACATGA CTCCAAGGGA CTCTGCGGGA 
    ACTCCTTCTA CTCCTCGTGA TGATAAGAAC CAAACACTCT CCGTTTCTGC ACCTGATCTT 
    GCTGCAGCAC GGCAACGACA AGCTTCAGCC GAAACAGACG GAGACGCAGA TGCGGATGAG 
    ACAAACTCTG AAGATAAAAC TGTTGGTGCT GATGATGCAA TGGAAGAAGA CGACGAAGAG 
    GAGGAAACAA TGGAGGACGA GGAAGATGAT GATGACGACG ATGATGATGA AAGCTCCAAC 
    GAAAACCAAG AAAAGCTAGT CGAATTGCTA GGTGGAGAAC GTGGCTTGTT TGATAAACTT 
    AAAGAAGTCA TCACCGGAGA ATCATTATCG GACGCTTCGT CGTCTGCCAA AGACGCTACC 
    ACCAATGAAG CTCAGAAGAA GGGAGGCAAA AAACCTAAAA AGTGGTTCAA AAAGATGTCC 
    AGCTACACGG ATGTTCTTAA AGGTTTAATG CAAAATCGTT ATCCTGTCGC TCTTCTCGAT 
    CCAGCTGCTG CCGGTATTGA AATGGATGAA ATGATGGATG ATGATGACTA CTATGATTTC 
    TCGGAAGATG GTCCAGATGA TGGTGATTCA GTTGAAGATG AAGTTGCTGC TCACCTTGGA 
    ATGCCTGTTG ACTCATTTGC ATCAATGGTT GCTGCCAGAA CCCCAATAAC ATGGCGTCAA 
    TTTTCTGAAT TAATGAGTGG TTCTAATCGC GAACGTGCTG CTATGGCAAG AGCTGTAGCA 
    TCGAGTCGAG GATCCCCATG GGAAGATGAT GCAACTGTCA AGTGTACATT CGAAGCCCTT 
    ATTCCAGCAT TCGATCCACG CCCAGGTCGT TCAAATGTGA ATCAAACTTT AGAAGTCGAA 
    CTTCCAACCG TCGTAAAAGA TTTCGGTTCT ACCAAGGCTT CTTCTTCAAA GATTGACAAA 
    GATGATCAAA TGCGCTTCTT CCTTCGTGGA CCAAACATGT CAGGTGTCGA TAATGTGACC 
    ATCGAGATGA ATGATGATTC AGCATCCCTG TTCCGATACA TGCAAATCAT TAACAACAGT 
    GTCAATTGGG CTACAAAAAG TGATCGATCT CGCCGGATCT GGGAGCCTAC GTACTCAATC 
    TGTTACTGCT CTGCCGATCA AACAAATGTC GAAGTCTCCA AAATTCCTGA TGAGGAAAGT 
    AGTACACCAT GTCAAGTAAA TCAATGTCTT GAAACCATCG GATTGCTTTC AAGAATTCAA 
    CAGGCAATGC CAGAAGCAGA GATAACTCCA AATGTGTTTA TCAGTGATAA ACTGACGTTG 
    AAAGTAACTC AAGTTCTCTC TGATGCTCTC GTTGTCGCAG CACGTTCTCT TCCTGAATGG 
    TGCTCTCGTT TAGTCTATAA ATATCCATGT CTGTTCACTG TGGAGACAAG AAACATGTAC 
    ATGCAGGCTA CTGCATTCGG CGTGTCTCGT ACAATCGTGT GGCTCCAACA ACGTCGTGAT 
    GCTGCTGTCG AACGTGCCCG CGGAAGTGCT CAAGCTGGAA ACAGTAGCGC TGCCCGGCAA 
    CATGATCGTT ATCACGAATA TCGGGTAGGG CGTCTTCGCC ATGAACGAGT TAAAGTGACT 
    CGAGCTGAAG AGACGCTTCT CGATCAAGCG ATCCGATTAA TGAAGTTCCA TGCTGATCGT 
    AAGGCGGTGC TAGAGATCGA ATACACCAAT GAAGAAGGAA CAGGATTGGG ACCAACTCTT 
    GAGTTCTATG CATTGGTTGC TGCAGAGTTG CAACGAAAGT CATTGGCGTT GTGGGTTTGT 
    GATGATGATG ATACACATGC ATCAAAGAGT GGAGAAGAAA GAGAAGTCGA TCTCGGTGAA 
    GGAAAGAAGC CAATTGGATA CTACGTTCGT CGCGTTGGTG GTCTCTTCCC AGCACCACTT 
    CCACCAGGAA CCGATGAAAC AAAGAGAGCA GCAGATATGT TCCGTGTTCT CGGAGTATTT 
    CTTGCTAAAG TTCTTCTCGA TGGTCGTCTT GTTGATCTAC CACTATCCCG TCCATTCCTC 
    AAGTTACTCG TCCATCCTCA GATTGGAGAT GATGCTCGTG GACCAAACTT GCACAAGATT 
    CTCAGCCTAG ACGATTTCGA AGAAGTGAAT CCAGTAAAAG GAAGTTTCCT TAAAGAGTTG 
    CGAGCTCTTG CTCAGCGTAA GCGACTGATC GAGAACGACA CGAGCATCGA TTCGAATTCC 
    AAGAGAAGAA AAATTGCGGA GCTCAAGTTA CACATTAAGG GATCCACATG CAGAGTTGAA 
    GATCTTGCAC TTAATTTCAC TGTCAATCCG CCATCAAAAG TGTTCCAATA TGCCGAAATG 
    GAGTTGGTTG ACGGTGGATC CGACATCGAT GTTACAATCG ACAACGTGGA GCAATATGTG 
    GAGAAATGTG AAGAATTCTA TTTGAACACA GGAATAGCCT ACCAGATGCG TGCTTTCCGT 
    GATGGATTCG ATCGCGTTTT TCCACTTAGA ACTCTCAGAG CATACTCTCC AGAAGAAGTT 
    CAGCGTCTTT TGTCCGGTGA ACAGTGCCCA GAATGGTCTC GGGACGATAT TCTAAATTAT 
    ACGGAGCCAA AACTTGGATA CACTCGAGAA TCTCCTGGTT TCTTGAGATT TGTCGATGTA 
    ATGGAAGCAT TGACAGCTCA AGAACGAAAG AATTTCCTTC AATTCGCCAC AGGATGTTCG 
    AGCTTGCCAC CAGGTGGTCT TGCTAATCTT CATCCAAGGT TGACAATTGT GCGAAAAGTG 
    GAAAGTGGAG ACGGCAGCTA TCCATCGGTT AATACATGTG TGCATTATCT CAAACTCCCG 
    GAGTACTCCT CGTCTGCAAT CCTCCGCGAA CGGCTTCTCA CGGCAATCAA CGAGAAAGGA 
    TTCCATTTGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306763.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293692.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342646
    Clone ID Related Accession (Same CDS sequence) NM_001306763.1 , NM_001306763.2
    Accession Version NM_001306763.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG 
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA 
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA 
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG 
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG 
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA 
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT 
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG 
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC 
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG 
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT 
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG 
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG 
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG 
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA 
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT 
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA 
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA 
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT 
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG 
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG 
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT 
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA 
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC 
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA 
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA 
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA 
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG 
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT 
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT 
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC 
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT 
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA 
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT 
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT 
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT 
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT 
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG 
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA 
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT 
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT 
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT 
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG 
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC 
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC 
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT 
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC 
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG 
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT 
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT 
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC 
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG 
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT 
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA 
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA 
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT 
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT 
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC 
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC 
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG 
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA 
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG 
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT 
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC 
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT 
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC 
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA 
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT 
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA 
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG 
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC 
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA 
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA 
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA 
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC 
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA 
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG 
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA 
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC 
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA 
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT 
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA 
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA 
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC 
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA 
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA 
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA 
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG 
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293692.1
    CDS1..7941
    Translation

    Target ORF information:

    RefSeq Version NM_001306763.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG 
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA 
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA 
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG 
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG 
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA 
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT 
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG 
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC 
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG 
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT 
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG 
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG 
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG 
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA 
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT 
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA 
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA 
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT 
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG 
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG 
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT 
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA 
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC 
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA 
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA 
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA 
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG 
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT 
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT 
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC 
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT 
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA 
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT 
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT 
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT 
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT 
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG 
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA 
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT 
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT 
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT 
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG 
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC 
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC 
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT 
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC 
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG 
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT 
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT 
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC 
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG 
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT 
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA 
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA 
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT 
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT 
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC 
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC 
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG 
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA 
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG 
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT 
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC 
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT 
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC 
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA 
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT 
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA 
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG 
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC 
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA 
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA 
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA 
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC 
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA 
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG 
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA 
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC 
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA 
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT 
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA 
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA 
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC 
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA 
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA 
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA 
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG 
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306763.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293692.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342646
    Clone ID Related Accession (Same CDS sequence) NM_001306763.1 , NM_001306763.2
    Accession Version NM_001306763.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306763.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG 
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA 
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA 
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG 
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG 
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA 
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT 
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG 
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC 
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG 
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT 
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG 
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG 
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG 
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA 
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT 
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA 
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA 
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT 
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG 
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG 
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT 
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA 
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC 
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA 
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA 
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA 
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG 
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT 
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT 
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC 
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT 
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA 
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT 
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT 
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT 
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT 
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG 
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA 
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT 
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT 
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT 
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG 
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC 
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC 
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT 
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC 
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG 
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT 
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT 
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC 
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG 
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT 
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA 
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA 
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT 
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT 
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC 
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC 
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG 
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA 
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG 
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT 
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC 
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT 
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC 
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA 
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT 
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA 
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG 
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC 
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA 
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA 
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA 
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC 
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA 
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG 
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA 
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC 
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA 
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT 
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA 
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA 
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC 
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA 
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA 
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA 
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG 
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293692.1
    CDS242..8182
    Translation

    Target ORF information:

    RefSeq Version NM_001306763.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306763.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    CTTGAAGTTC CGGCCTGGGA GCTATGGTCC TCCAAATCTT CTGAACTCCA AATTAAATCC 
    ATTTCTTCAT CGGCACCAAC AGGACAAGCG AACACTATGC TGACAACAAT TAAAATTCAG 
    GACGATGCTG GTGGTTTCCT TTTCGAAACT GGAACTGGAC GAAAAACGAA TGTTATGCCA 
    GAGCATGCAT TACCATCGGA TTTCCATACT GGATGGAGTT CACACGGAGT ATCAACCCGA 
    AAAATGAAGT TCCGTCAAGA TATTCAGAAG CGTAAAGTTC AAGAATTGGC CTGGAAATTG 
    TGGAACGATC ATCTGAAAGA GGCCCATGCG AAGCCGAGAG AGGCACTTGT TAGATTGGAG 
    AATGCAGCAC GTACCATAGA GAGCACTATT CGACACGTTA AAGCTCAGAG CAACTTCAAA 
    CATCGGAATG TGAAGCAACC AAGAATTGAA AGAGTACAAG AATATTGTGC TGCCATCAGT 
    ACACTTCATG AGTCAATCGT TGATGATCGT CGTTTGTCTA CATTCGAATT TTCTGTTTCG 
    GGAATTGTGC CTGCGCTTTT TGGCCTTTTA TCAATGATGG AAAAGTTTCC AGACAGTTTC 
    CCATCAAGGA TCTTCAAGGA ACAATTCTCA AAAGGAGAAG CACTTTCATA TCTTGCACTG 
    AAAATAGTTG CTGTTTTGGA AGCGAATGAA AAGTTCCCAC AGCATTTATA CGATAGTCCT 
    GGAGGTTCTT CCTTCGGCCT TCAACTTCTG TCTCGTCGTG TGCGCACAAA ACTCGAAATG 
    CTTCCACGTG CTGATGGAAA GGAGAATAAC GACGAAAATC TTGTGAACAA GACAGGAAAG 
    ATAGTAAAGT GCGAACCTCT TGCATCTGTG GGAGCCATCA GAGCGTATCT TCATAGAATG 
    GTTACTCGAC AGTGGCATGA TCGTGAACGA GCCAATTATA GATATGTCAA AGAGATTCAA 
    GAGTTGAAGA CAAAAGGAAA ATCTATTGAA TTGAGACATG TTTCTGACTT TGATGAGAAT 
    GGTGTTATTT ATTGGATCGG AACAAATGGA AGAGCTGCAC CACTATGGAC AAATCCAGCA 
    ACCGTAAAAG CAGTTAAAAT CACTTGTTCG GATACTCGCC AACCATTCGG TAAACCTGAA 
    GATCTTCTGT CACGTGATCA AAATCCTATC AATTGTCATA CTTCCGATGA TAAAAATGCT 
    CATTTCACAA TTGATTTGGG TCTGTTCGTT GTTCCAACTT CTTATTCTCT TCGTCACTCG 
    CGTGGATATG GAAGATCTGC TCTGCGTAAT TGGATGCTTC AAGGATCTGT TGATGCAAAG 
    CGATGGGAAA ATGTGATTGT TCACACTGAC GATAAAGGAC TCGGAGAACC AGGATCTACT 
    GCTACTTGGC ATGTTGGTGA AAAGGGAACA ACCGCATTCA GATTCTTCAG AATTGCTCAA 
    AACGGAAAGA ACTCTTCGGG TCAAACACAT TACTTGTCTT GTTCCGGATT CGAGATTTAC 
    GGAGACATCG TTGATGTGGT AACAGAAGCC ATTTGCGAAG ACCCACCGAA AAAGGATTCA 
    CCAGCCGGTA CGTCGTCAAC TCCTGGATCC TCATCATCAG CCGCATTACC TCCACTCACA 
    AAAGAACAAG TGCTTGAAAT GCTTCCAGCT CGTGAGAACA ACAATCGTCT TAAATCAGGA 
    CTATCGCTGG AGACTGTCAC TTCAATGCTG CAGAGGTCCC GTCATCGTTC AAGAGGATCG 
    TACAAAATAA GCGAGTCGAA GTCAAAAGTT GTACGTGGAA AAGACTGGAG ATGGGAAGAT 
    CAGGATGGCG GAGAAGGAAA ATTCGGTCGT ATCACTAGTC CGCCAGAAAG TGGCTGGGTT 
    GATGTGACAT GGGACAATGG AAACGCCAAT TCATATCGAT TCGGAGCCAA TGGAAACTTC 
    GACATTGAGC GCGTTACCAG CACTGGACAT CGTTATTCAA CACCATCGCT AGCTTCACAT 
    GTTCCAACTT CGGTTATGGA GGCAGTTCGA CGAAATCGTG CTTTCTACAC TCCAAAAACA 
    ACTGGCGGTC CACCATCATC ATCCGTTTTT GGCACTTCAT CATCAGCTGG ATCTTCTCGT 
    GGAGCTGCTT CTGCTCTCTC GCGATTTGCA TCAGTGAAAA ACACTACTCC AGCTGGAACT 
    CCATCATCGG GAGGATCGTC TGGAGGTGCA ATCGGAAAGA AATCAATGTC AACCACAAAT 
    CTCGTCGATG AGCGACAGAA GACATCTGGA CCATCCGTTG CTTCAACAGG TCAAGCAGCT 
    TCTGCTGAAT CTCTTCAACA TCAAACTCCT TCTCTGGAGA ATCTTCTTGC AAGGGCCATG 
    CCACACGCAT TTGGACGTAT CGCAGAGAAT CAAGAACCCG AAGATGAACC AATGGGTGGA 
    GAAGAGAGTG ATTCGGCTGC AAGCATGAGA TCAGCAGCAT CGTCAAATTC ACAAATGTCT 
    ATGGGATCAT CTTCTCAACA ACAACAACAA CAAGATTCAG ACATGACTCC AAGGGACTCT 
    GCGGGAACTC CTTCTACTCC TCGTGATGAT AAGAACCAAA CACTCTCCGT TTCTGCACCT 
    GATCTTGCTG CAGCACGGCA ACGACAAGCT TCAGCCGAAA CAGACGGAGA CGCAGATGCG 
    GATGAGACAA ACTCTGAAGA TAAAACTGTT GGTGCTGATG ATGCAATGGA AGAAGACGAC 
    GAAGAGGAGG AAACAATGGA GGACGAGGAA GATGATGATG ACGACGATGA TGATGAAAGC 
    TCCAACGAAA ACCAAGAAAA GCTAGTCGAA TTGCTAGGTG GAGAACGTGG CTTGTTTGAT 
    AAACTTAAAG AAGTCATCAC CGGAGAATCA TTATCGGACG CTTCGTCGTC TGCCAAAGAC 
    GCTACCACCA ATGAAGCTCA GAAGAAGGGA GGCAAAAAAC CTAAAAAGTG GTTCAAAAAG 
    ATGTCCAGCT ACACGGATGT TCTTAAAGGT TTAATGCAAA ATCGTTATCC TGTCGCTCTT 
    CTCGATCCAG CTGCTGCCGG TATTGAAATG GATGAAATGA TGGATGATGA TGACTACTAT 
    GATTTCTCGG AAGATGGTCC AGATGATGGT GATTCAGTTG AAGATGAAGT TGCTGCTCAC 
    CTTGGAATGC CTGTTGACTC ATTTGCATCA ATGGTTGCTG CCAGAACCCC AATAACATGG 
    CGTCAATTTT CTGAATTAAT GAGTGGTTCT AATCGCGAAC GTGCTGCTAT GGCAAGAGCT 
    GTAGCATCGA GTCGAGGATC CCCATGGGAA GATGATGCAA CTGTCAAGTG TACATTCGAA 
    GCCCTTATTC CAGCATTCGA TCCACGCCCA GGTCGTTCAA ATGTGAATCA AACTTTAGAA 
    GTCGAACTTC CAACCGTCGT AAAAGATTTC GGTTCTACCA AGGCTTCTTC TTCAAAGATT 
    GACAAAGATG ATCAAATGCG CTTCTTCCTT CGTGGACCAA ACATGTCAGG TGTCGATAAT 
    GTGACCATCG AGATGAATGA TGATTCAGCA TCCCTGTTCC GATACATGCA AATCATTAAC 
    AACAGTGTCA ATTGGGCTAC AAAAAGTGAT CGATCTCGCC GGATCTGGGA GCCTACGTAC 
    TCAATCTGTT ACTGCTCTGC CGATCAAACA AATGTCGAAG TCTCCAAAAT TCCTGATGAG 
    GAAAGTAGTA CACCATGTCA AGTAAATCAA TGTCTTGAAA CCATCGGATT GCTTTCAAGA 
    ATTCAACAGG CAATGCCAGA AGCAGAGATA ACTCCAAATG TGTTTATCAG TGATAAACTG 
    ACGTTGAAAG TAACTCAAGT TCTCTCTGAT GCTCTCGTTG TCGCAGCACG TTCTCTTCCT 
    GAATGGTGCT CTCGTTTAGT CTATAAATAT CCATGTCTGT TCACTGTGGA GACAAGAAAC 
    ATGTACATGC AGGCTACTGC ATTCGGCGTG TCTCGTACAA TCGTGTGGCT CCAACAACGT 
    CGTGATGCTG CTGTCGAACG TGCCCGCGGA AGTGCTCAAG CTGGAAACAG TAGCGCTGCC 
    CGGCAACATG ATCGTTATCA CGAATATCGG GTAGGGCGTC TTCGCCATGA ACGAGTTAAA 
    GTGACTCGAG CTGAAGAGAC GCTTCTCGAT CAAGCGATCC GATTAATGAA GTTCCATGCT 
    GATCGTAAGG CGGTGCTAGA GATCGAATAC ACCAATGAAG AAGGAACAGG ATTGGGACCA 
    ACTCTTGAGT TCTATGCATT GGTTGCTGCA GAGTTGCAAC GAAAGTCATT GGCGTTGTGG 
    GTTTGTGATG ATGATGATAC ACATGCATCA AAGAGTGGAG AAGAAAGAGA AGTCGATCTC 
    GGTGAAGGAA AGAAGCCAAT TGGATACTAC GTTCGTCGCG TTGGTGGTCT CTTCCCAGCA 
    CCACTTCCAC CAGGAACCGA TGAAACAAAG AGAGCAGCAG ATATGTTCCG TGTTCTCGGA 
    GTATTTCTTG CTAAAGTTCT TCTCGATGGT CGTCTTGTTG ATCTACCACT ATCCCGTCCA 
    TTCCTCAAGT TACTCGTCCA TCCTCAGATT GGAGATGATG CTCGTGGACC AAACTTGCAC 
    AAGATTCTCA GCCTAGACGA TTTCGAAGAA GTGAATCCAG TAAAAGGAAG TTTCCTTAAA 
    GAGTTGCGAG CTCTTGCTCA GCGTAAGCGA CTGATCGAGA ACGACACGAG CATCGATTCG 
    AATTCCAAGA GAAGAAAAAT TGCGGAGCTC AAGTTACACA TTAAGGGATC CACATGCAGA 
    GTTGAAGATC TTGCACTTAA TTTCACTGTC AATCCGCCAT CAAAAGTGTT CCAATATGCC 
    GAAATGGAGT TGGTTGACGG TGGATCCGAC ATCGATGTTA CAATCGACAA CGTGGAGCAA 
    TATGTGGAGA AATGTGAAGA ATTCTATTTG AACACAGGAA TAGCCTACCA GATGCGTGCT 
    TTCCGTGATG GATTCGATCG CGTTTTTCCA CTTAGAACTC TCAGAGCATA CTCTCCAGAA 
    GAAGTTCAGC GTCTTTTGTC CGGTGAACAG TGCCCAGAAT GGTCTCGGGA CGATATTCTA 
    AATTATACGG AGCCAAAACT TGGATACACT CGAGAATCTC CTGGTTTCTT GAGATTTGTC 
    GATGTAATGG AAGCATTGAC AGCTCAAGAA CGAAAGAATT TCCTTCAATT CGCCACAGGA 
    TGTTCGAGCT TGCCACCAGG TGGTCTTGCT AATCTTCATC CAAGGTTGAC AATTGTGCGA 
    AAAGTGGAAA GTGGAGACGG CAGCTATCCA TCGGTTAATA CATGTGTGCA TTATCTCAAA 
    CTCCCGGAGT ACTCCTCGTC TGCAATCCTC CGCGAACGGC TTCTCACGGC AATCAACGAG 
    AAAGGATTCC ATTTGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306764.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293693.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342647
    Clone ID Related Accession (Same CDS sequence) NM_001306764.2 , NM_001306764.1
    Accession Version NM_001306764.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306764.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG 
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA 
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG 
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT 
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG 
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT 
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA 
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT 
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA 
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA 
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA 
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC 
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT 
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC 
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC 
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG 
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC 
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT 
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT 
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT 
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA 
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC 
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA 
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG 
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA 
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT 
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT 
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG 
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA 
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC 
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG 
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC 
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT 
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT 
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG 
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA 
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT 
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA 
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT 
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT 
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC 
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA 
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG 
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT 
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT 
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG 
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG 
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT 
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT 
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA 
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC 
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT 
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG 
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT 
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT 
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA 
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG 
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG 
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA 
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA 
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC 
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA 
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG 
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG 
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC 
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT 
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG 
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA 
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA 
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA 
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT 
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC 
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA 
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA 
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA 
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG 
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA 
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG 
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC 
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC 
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA 
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG 
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC 
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA 
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG 
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG 
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG 
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293693.1
    CDS242..8194
    Translation

    Target ORF information:

    RefSeq Version NM_001306764.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG 
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA 
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG 
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT 
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG 
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT 
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA 
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT 
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA 
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA 
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA 
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC 
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT 
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC 
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC 
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG 
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC 
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT 
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT 
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT 
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA 
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC 
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA 
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG 
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA 
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT 
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT 
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG 
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA 
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC 
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG 
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC 
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT 
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT 
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG 
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA 
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT 
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA 
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT 
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT 
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC 
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA 
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG 
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT 
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT 
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG 
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG 
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT 
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT 
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA 
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC 
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT 
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG 
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT 
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT 
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA 
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG 
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG 
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA 
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA 
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC 
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA 
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG 
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG 
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC 
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT 
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG 
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA 
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA 
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA 
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT 
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC 
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA 
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA 
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA 
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG 
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA 
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG 
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC 
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC 
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA 
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG 
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC 
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA 
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG 
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG 
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG 
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306764.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293693.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342647
    Clone ID Related Accession (Same CDS sequence) NM_001306764.2 , NM_001306764.1
    Accession Version NM_001306764.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG 
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA 
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG 
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT 
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG 
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT 
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA 
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT 
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA 
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA 
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA 
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC 
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT 
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC 
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC 
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG 
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC 
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT 
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT 
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT 
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA 
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC 
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA 
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG 
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA 
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT 
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT 
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG 
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA 
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC 
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG 
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC 
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT 
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT 
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG 
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA 
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT 
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA 
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT 
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT 
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC 
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA 
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG 
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT 
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT 
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG 
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG 
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT 
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT 
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA 
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC 
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT 
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG 
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT 
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT 
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA 
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG 
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG 
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA 
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA 
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC 
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA 
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG 
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG 
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC 
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT 
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG 
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA 
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA 
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA 
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT 
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC 
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA 
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA 
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA 
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG 
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA 
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG 
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC 
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC 
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA 
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG 
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC 
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA 
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG 
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG 
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG 
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293693.1
    CDS1..7953
    Translation

    Target ORF information:

    RefSeq Version NM_001306764.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG GTACAGTATT TGTGTGATAA GGGGTCCGAT 
    GTCAATAAGG GGCACAAGAG CAGTTCTTTG CACTATGCTG CTTGTTTTGG ACGGCCGGAT 
    GTTGTGAAAT TGCTGCTTCA GCGTGGCGCC AATCCTGATC TACGAGACGA AGATGGCAAG 
    ACAGCTTTGG ACAAGGCACG AGAAAGATCT GATGATGATC ACAATCAAGT CGCCAACATT 
    CTTGAATCGC CTAGCGCATT CATGAGAAAT AAAGAAGATC CGAAGGTGAA AGCATCAACG 
    TCGAAACAGC CAGGTACTTC AACGAAACCT GAGCTTCCAA ATCCAAACCT TGTTCGCAAA 
    GTTTTGCATC AACTTCTTCC AATATTCTGT GAGATATTCC AAAAGTCTCT CAACGGAAGT 
    GTTCGTCGCA CATCACTGTC TCTGATGCGA AAAATTGTGG AAAACATTGG AGATCTTCGT 
    CAAAGCGCCG TGGGAGATGG CAATGCTCCA GCTGCACAGT CCGCACGAAA AATGTCTACA 
    GATGTGTCTG CTGGTGCTGA ATCTTTGGTA GCTGTTGTCG TTTCTGTAAT GGATCAAGAA 
    GATGATCATG AGGGTCACGA ACAAGTGCTT CTTATTCTGG AATCTCTTTT GGAAAAAGAT 
    GCCGAACTCT GGGTTATTGA ATTGGTGCGA CTTGGTGTGT TTGAACGTGT CGAGGCGATG 
    GCAAAAGAGC CACCAAAGGG ATTGGAGGAA GTTTTGAATG CAATTCATTT GGAGGGAAGA 
    TCTAGAGTTA CACCCATGGA AATTGACTTT GAGAATCAAC CCTCGTCATC AACAGCTGTT 
    CCTACGGCGA ACGACATCAT GGATACCACT GTTCCATCTT CTTCTGGTGG AGCCGACGCT 
    GAATCAAACT CGAATCCTTC TACAATTGAG ATGGCTGATC CAGAATCATC AACTCCTTCG 
    TCATCCACAC AGCAATCTAT TTCTAAACCA AAAGCAACAG CCAGTTCAAC AGCATCTTCA 
    GCTATTCTTC AAGTGGTATC CAAATTGAGT AGTGTTGCCA GTCTCGATAA ATCAGCTGCC 
    GCAGTTGATA AGAAACCAAC AAAGACAGTA TTGAGCCAGG GAACACCTTA TCGTTGGAAA 
    GAATGGCGTA TCGTACGCGG AACCACATCT CTATTCATCT GGAGTGATGT CCTGCTCATT 
    GAGTTACCAT TCCAATCTAA TGGATGGTTC CGTTATTTAG CCGATAATGA CTCACATGTA 
    CAGTTCGTCA CTGGTACTGC AAGTGTTGAT CAGCAAATGA CCGAGGAAGA AAAGGATAAT 
    TTCCAAAAAA CCGAACGACG CGAAATGGTT TCCCGTTGGA ATGCAGTAAA AGGAGTTTTC 
    GATGACGATT GGTCTTCTGT TCCAATTGCT GTTCTCGGAA TTCCCAGTAA CGCTAAGAAG 
    GTCAGTCAGA AGCTTGAAGT TCCGGCCTGG GAGCTATGGT CCTCCAAATC TTCTGAACTC 
    CAAATTAAAT CCATTTCTTC ATCGGCACCA ACAGGACAAG CGAACACTAT GCTGACAACA 
    ATTAAAATTC AGGACGATGC TGGTGGTTTC CTTTTCGAAA CTGGAACTGG ACGAAAAACG 
    AATGTTATGC CAGAGCATGC ATTACCATCG GATTTCCATA CTGGATGGAG TTCACACGGA 
    GTATCAACCC GAAAAATGAA GTTCCGTCAA GATATTCAGA AGCGTAAAGT TCAAGAATTG 
    GCCTGGAAAT TGTGGAACGA TCATCTGAAA GAGGCCCATG CGAAGCCGAG AGAGGCACTT 
    GTTAGATTGG AGAATGCAGC ACGTACCATA GAGAGCACTA TTCGACACGT TAAAGCTCAG 
    AGCAACTTCA AACATCGGAA TGTGAAGCAA CCAAGAATTG AAAGAGTACA AGAATATTGT 
    GCTGCCATCA GTACACTTCA TGAGTCAATC GTTGATGATC GTCGTTTGTC TACATTCGAA 
    TTTTCTGTTT CGGGAATTGT GCCTGCGCTT TTTGGCCTTT TATCAATGAT GGAAAAGTTT 
    CCAGACAGTT TCCCATCAAG GATCTTCAAG GAACAATTCT CAAAAGGAGA AGCACTTTCA 
    TATCTTGCAC TGAAAATAGT TGCTGTTTTG GAAGCGAATG AAAAGTTCCC ACAGCATTTA 
    TACGATAGTC CTGGAGGTTC TTCCTTCGGC CTTCAACTTC TGTCTCGTCG TGTGCGCACA 
    AAACTCGAAA TGCTTCCACG TGCTGATGGA AAGGAGAATA ACGACGAAAA TCTTGTGAAC 
    AAGACAGGAA AGATAGTAAA GTGCGAACCT CTTGCATCTG TGGGAGCCAT CAGAGCGTAT 
    CTTCATAGAA TGGTTACTCG ACAGTGGCAT GATCGTGAAC GAGCCAATTA TAGATATGTC 
    AAAGAGATTC AAGAGTTGAA GACAAAAGGA AAATCTATTG AATTGAGACA TGTTTCTGAC 
    TTTGATGAGA ATGGTGTTAT TTATTGGATC GGAACAAATG GAAGAGCTGC ACCACTATGG 
    ACAAATCCAG CAACCGTAAA AGCAGTTAAA ATCACTTGTT CGGATACTCG CCAACCATTC 
    GGTAAACCTG AAGATCTTCT GTCACGTGAT CAAAATCCTA TCAATTGTCA TACTTCCGAT 
    GATAAAAATG CTCATTTCAC AATTGATTTG GGTCTGTTCG TTGTTCCAAC TTCTTATTCT 
    CTTCGTCACT CGCGTGGATA TGGAAGATCT GCTCTGCGTA ATTGGATGCT TCAAGGATCT 
    GTTGATGCAA AGCGATGGGA AAATGTGATT GTTCACACTG ACGATAAAGG ACTCGGAGAA 
    CCAGGATCTA CTGCTACTTG GCATGTTGGT GAAAAGGGAA CAACCGCATT CAGATTCTTC 
    AGAATTGCTC AAAACGGAAA GAACTCTTCG GGTCAAACAC ATTACTTGTC TTGTTCCGGA 
    TTCGAGATTT ACGGAGACAT CGTTGATGTG GTAACAGAAG CCATTTGCGA AGACCCACCG 
    AAAAAGGATT CACCAGCCGG TACGTCGTCA ACTCCTGGAT CCTCATCATC AGCCGCATTA 
    CCTCCACTCA CAAAAGAACA AGTGCTTGAA ATGCTTCCAG CTCGTGAGAA CAACAATCGT 
    CTTAAATCAG GACTATCGCT GGAGACTGTC ACTTCAATGC TGCAGAGGTC CCGTCATCGT 
    TCAAGAGGAT CGTACAAAAT AAGCGAGTCG AAGTCAAAAG TTGTACGTGG AAAAGACTGG 
    AGATGGGAAG ATCAGGATGG CGGAGAAGGA AAATTCGGTC GTATCACTAG TCCGCCAGAA 
    AGTGGCTGGG TTGATGTGAC ATGGGACAAT GGAAACGCCA ATTCATATCG ATTCGGAGCC 
    AATGGAAACT TCGACATTGA GCGCGTTACC AGCACTGGAC ATCGTTATTC AACACCATCG 
    CTAGCTTCAC ATGTTCCAAC TTCGGTTATG GAGGCAGTTC GACGAAATCG TGCTTTCTAC 
    ACTCCAAAAA CAACTGGCGG TCCACCATCA TCATCCGTTT TTGGCACTTC ATCATCAGCT 
    GGATCTTCTC GTGGAGCTGC TTCTGCTCTC TCGCGATTTG CATCAGTGAA AAACACTACT 
    CCAGCTGGAA CTCCATCATC GGGAGGATCG TCTGGAGGTG CAATCGGAAA GAAATCAATG 
    TCAACCACAA ATCTCGTCGA TGAGCGACAG AAGACATCTG GACCATCCGT TGCTTCAACA 
    GGTCAAGCAG CTTCTGCTGA ATCTCTTCAA CATCAAACTC CTTCTCTGGA GAATCTTCTT 
    GCAAGGGCCA TGCCACACGC ATTTGGACGT ATCGCAGAGA ATCAAGAACC CGAAGATGAA 
    CCAATGGGTG GAGAAGAGAG TGATTCGGCT GCAAGCATGA GATCAGCAGC ATCGTCAAAT 
    TCACAAATGT CTATGGGATC ATCTTCTCAA CAACAACAAC AACAAGATTC AGACATGACT 
    CCAAGGGACT CTGCGGGAAC TCCTTCTACT CCTCGTGATG ATAAGAACCA AACACTCTCC 
    GTTTCTGCAC CTGATCTTGC TGCAGCACGG CAACGACAAG CTTCAGCCGA AACAGACGGA 
    GACGCAGATG CGGATGAGAC AAACTCTGAA GATAAAACTG TTGGTGCTGA TGATGCAATG 
    GAAGAAGACG ACGAAGAGGA GGAAACAATG GAGGACGAGG AAGATGATGA TGACGACGAT 
    GATGATGAAA GCTCCAACGA AAACCAAGAA AAGCTAGTCG AATTGCTAGG TGGAGAACGT 
    GGCTTGTTTG ATAAACTTAA AGAAGTCATC ACCGGAGAAT CATTATCGGA CGCTTCGTCG 
    TCTGCCAAAG ACGCTACCAC CAATGAAGCT CAGAAGAAGG GAGGCAAAAA ACCTAAAAAG 
    TGGTTCAAAA AGATGTCCAG CTACACGGAT GTTCTTAAAG GTTTAATGCA AAATCGTTAT 
    CCTGTCGCTC TTCTCGATCC AGCTGCTGCC GGTATTGAAA TGGATGAAAT GATGGATGAT 
    GATGACTACT ATGATTTCTC GGAAGATGGT CCAGATGATG GTGATTCAGT TGAAGATGAA 
    GTTGCTGCTC ACCTTGGAAT GCCTGTTGAC TCATTTGCAT CAATGGTTGC TGCCAGAACC 
    CCAATAACAT GGCGTCAATT TTCTGAATTA ATGAGTGGTT CTAATCGCGA ACGTGCTGCT 
    ATGGCAAGAG CTGTAGCATC GAGTCGAGGA TCCCCATGGG AAGATGATGC AACTGTCAAG 
    TGTACATTCG AAGCCCTTAT TCCAGCATTC GATCCACGCC CAGGTCGTTC AAATGTGAAT 
    CAAACTTTAG AAGTCGAACT TCCAACCGTC GTAAAAGATT TCGGTTCTAC CAAGGCTTCT 
    TCTTCAAAGA TTGACAAAGA TGATCAAATG CGCTTCTTCC TTCGTGGACC AAACATGTCA 
    GGTGTCGATA ATGTGACCAT CGAGATGAAT GATGATTCAG CATCCCTGTT CCGATACATG 
    CAAATCATTA ACAACAGTGT CAATTGGGCT ACAAAAAGTG ATCGATCTCG CCGGATCTGG 
    GAGCCTACGT ACTCAATCTG TTACTGCTCT GCCGATCAAA CAAATGTCGA AGTCTCCAAA 
    ATTCCTGATG AGGAAAGTAG TACACCATGT CAAGTAAATC AATGTCTTGA AACCATCGGA 
    TTGCTTTCAA GAATTCAACA GGCAATGCCA GAAGCAGAGA TAACTCCAAA TGTGTTTATC 
    AGTGATAAAC TGACGTTGAA AGTAACTCAA GTTCTCTCTG ATGCTCTCGT TGTCGCAGCA 
    CGTTCTCTTC CTGAATGGTG CTCTCGTTTA GTCTATAAAT ATCCATGTCT GTTCACTGTG 
    GAGACAAGAA ACATGTACAT GCAGGCTACT GCATTCGGCG TGTCTCGTAC AATCGTGTGG 
    CTCCAACAAC GTCGTGATGC TGCTGTCGAA CGTGCCCGCG GAAGTGCTCA AGCTGGAAAC 
    AGTAGCGCTG CCCGGCAACA TGATCGTTAT CACGAATATC GGGTAGGGCG TCTTCGCCAT 
    GAACGAGTTA AAGTGACTCG AGCTGAAGAG ACGCTTCTCG ATCAAGCGAT CCGATTAATG 
    AAGTTCCATG CTGATCGTAA GGCGGTGCTA GAGATCGAAT ACACCAATGA AGAAGGAACA 
    GGATTGGGAC CAACTCTTGA GTTCTATGCA TTGGTTGCTG CAGAGTTGCA ACGAAAGTCA 
    TTGGCGTTGT GGGTTTGTGA TGATGATGAT ACACATGCAT CAAAGAGTGG AGAAGAAAGA 
    GAAGTCGATC TCGGTGAAGG AAAGAAGCCA ATTGGATACT ACGTTCGTCG CGTTGGTGGT 
    CTCTTCCCAG CACCACTTCC ACCAGGAACC GATGAAACAA AGAGAGCAGC AGATATGTTC 
    CGTGTTCTCG GAGTATTTCT TGCTAAAGTT CTTCTCGATG GTCGTCTTGT TGATCTACCA 
    CTATCCCGTC CATTCCTCAA GTTACTCGTC CATCCTCAGA TTGGAGATGA TGCTCGTGGA 
    CCAAACTTGC ACAAGATTCT CAGCCTAGAC GATTTCGAAG AAGTGAATCC AGTAAAAGGA 
    AGTTTCCTTA AAGAGTTGCG AGCTCTTGCT CAGCGTAAGC GACTGATCGA GAACGACACG 
    AGCATCGATT CGAATTCCAA GAGAAGAAAA ATTGCGGAGC TCAAGTTACA CATTAAGGGA 
    TCCACATGCA GAGTTGAAGA TCTTGCACTT AATTTCACTG TCAATCCGCC ATCAAAAGTG 
    TTCCAATATG CCGAAATGGA GTTGGTTGAC GGTGGATCCG ACATCGATGT TACAATCGAC 
    AACGTGGAGC AATATGTGGA GAAATGTGAA GAATTCTATT TGAACACAGG AATAGCCTAC 
    CAGATGCGTG CTTTCCGTGA TGGATTCGAT CGCGTTTTTC CACTTAGAAC TCTCAGAGCA 
    TACTCTCCAG AAGAAGTTCA GCGTCTTTTG TCCGGTGAAC AGTGCCCAGA ATGGTCTCGG 
    GACGATATTC TAAATTATAC GGAGCCAAAA CTTGGATACA CTCGAGAATC TCCTGGTTTC 
    TTGAGATTTG TCGATGTAAT GGAAGCATTG ACAGCTCAAG AACGAAAGAA TTTCCTTCAA 
    TTCGCCACAG GATGTTCGAG CTTGCCACCA GGTGGTCTTG CTAATCTTCA TCCAAGGTTG 
    ACAATTGTGC GAAAAGTGGA AAGTGGAGAC GGCAGCTATC CATCGGTTAA TACATGTGTG 
    CATTATCTCA AACTCCCGGA GTACTCCTCG TCTGCAATCC TCCGCGAACG GCTTCTCACG 
    GCAATCAACG AGAAAGGATT CCATTTGAAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306765.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293694.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342648
    Clone ID Related Accession (Same CDS sequence) NM_001306765.1 , NM_001306765.2
    Accession Version NM_001306765.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

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    1021
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    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA 
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT 
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT 
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA 
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT 
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA 
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT 
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG 
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA 
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA 
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT 
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT 
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC 
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT 
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT 
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA 
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC 
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT 
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT 
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT 
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA 
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC 
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT 
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA 
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA 
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC 
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC 
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA 
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT 
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA 
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA 
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT 
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA 
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA 
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG 
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT 
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG 
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC 
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA 
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA 
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA 
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA 
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT 
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT 
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT 
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC 
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA 
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA 
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG 
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT 
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT 
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA 
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA 
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA 
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC 
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA 
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC 
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC 
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA 
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA 
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT 
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT 
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG 
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA 
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA 
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT 
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC 
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA 
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA 
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA 
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC 
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA 
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT 
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT 
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA 
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG 
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC 
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA 
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT 
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA 
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT 
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA 
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT 
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT 
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT 
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT 
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG 
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293694.1
    CDS1..7842
    Translation

    Target ORF information:

    RefSeq Version NM_001306765.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA 
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT 
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT 
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA 
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT 
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA 
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT 
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG 
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA 
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA 
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT 
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT 
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC 
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT 
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT 
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA 
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC 
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT 
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT 
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT 
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA 
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC 
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT 
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA 
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA 
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC 
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC 
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA 
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT 
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA 
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA 
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT 
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA 
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA 
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG 
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT 
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG 
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC 
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA 
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA 
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA 
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA 
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT 
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT 
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT 
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC 
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA 
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA 
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG 
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT 
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT 
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA 
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA 
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA 
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC 
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA 
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC 
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC 
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA 
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA 
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT 
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT 
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG 
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA 
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA 
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT 
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC 
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA 
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA 
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA 
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC 
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA 
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT 
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT 
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA 
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG 
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC 
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA 
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT 
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA 
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT 
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA 
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT 
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT 
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT 
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT 
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG 
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306765.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293694.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342648
    Clone ID Related Accession (Same CDS sequence) NM_001306765.1 , NM_001306765.2
    Accession Version NM_001306765.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306765.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA 
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT 
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT 
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA 
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT 
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA 
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT 
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG 
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA 
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA 
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT 
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT 
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC 
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT 
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT 
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA 
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC 
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT 
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT 
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT 
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA 
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC 
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT 
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA 
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA 
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC 
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC 
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA 
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT 
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA 
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA 
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT 
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA 
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA 
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG 
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT 
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG 
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC 
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA 
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA 
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA 
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA 
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT 
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT 
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT 
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC 
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA 
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA 
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG 
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT 
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT 
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA 
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA 
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA 
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC 
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA 
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC 
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC 
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA 
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA 
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT 
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT 
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG 
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA 
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA 
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT 
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC 
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA 
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA 
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA 
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC 
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA 
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT 
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT 
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA 
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG 
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC 
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA 
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT 
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA 
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT 
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA 
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT 
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT 
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT 
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT 
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG 
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293694.1
    CDS242..8083
    Translation

    Target ORF information:

    RefSeq Version NM_001306765.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306765.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGG TCAGTCAGAA GCTTGAAGTT CCGGCCTGGG AGCTATGGTC CTCCAAATCT 
    TCTGAACTCC AAATTAAATC CATTTCTTCA TCGGCACCAA CAGGACAAGC GAACACTATG 
    CTGACAACAA TTAAAATTCA GGACGATGCT GGTGGTTTCC TTTTCGAAAC TGGAACTGGA 
    CGAAAAACGA ATGTTATGCC AGAGCATGCA TTACCATCGG ATTTCCATAC TGGATGGAGT 
    TCACACGGAG TATCAACCCG AAAAATGAAG TTCCGTCAAG ATATTCAGAA GCGTAAAGTT 
    CAAGAATTGG CCTGGAAATT GTGGAACGAT CATCTGAAAG AGGCCCATGC GAAGCCGAGA 
    GAGGCACTTG TTAGATTGGA GAATGCAGCA CGTACCATAG AGAGCACTAT TCGACACGTT 
    AAAGCTCAGA GCAACTTCAA ACATCGGAAT GTGAAGCAAC CAAGAATTGA AAGAGTACAA 
    GAATATTGTG CTGCCATCAG TACACTTCAT GAGTCAATCG TTGATGATCG TCGTTTGTCT 
    ACATTCGAAT TTTCTGTTTC GGGAATTGTG CCTGCGCTTT TTGGCCTTTT ATCAATGATG 
    GAAAAGTTTC CAGACAGTTT CCCATCAAGG ATCTTCAAGG AACAATTCTC AAAAGGAGAA 
    GCACTTTCAT ATCTTGCACT GAAAATAGTT GCTGTTTTGG AAGCGAATGA AAAGTTCCCA 
    CAGCATTTAT ACGATAGTCC TGGAGGTTCT TCCTTCGGCC TTCAACTTCT GTCTCGTCGT 
    GTGCGCACAA AACTCGAAAT GCTTCCACGT GCTGATGGAA AGGAGAATAA CGACGAAAAT 
    CTTGTGAACA AGACAGGAAA GATAGTAAAG TGCGAACCTC TTGCATCTGT GGGAGCCATC 
    AGAGCGTATC TTCATAGAAT GGTTACTCGA CAGTGGCATG ATCGTGAACG AGCCAATTAT 
    AGATATGTCA AAGAGATTCA AGAGTTGAAG ACAAAAGGAA AATCTATTGA ATTGAGACAT 
    GTTTCTGACT TTGATGAGAA TGGTGTTATT TATTGGATCG GAACAAATGG AAGAGCTGCA 
    CCACTATGGA CAAATCCAGC AACCGTAAAA GCAGTTAAAA TCACTTGTTC GGATACTCGC 
    CAACCATTCG GTAAACCTGA AGATCTTCTG TCACGTGATC AAAATCCTAT CAATTGTCAT 
    ACTTCCGATG ATAAAAATGC TCATTTCACA ATTGATTTGG GTCTGTTCGT TGTTCCAACT 
    TCTTATTCTC TTCGTCACTC GCGTGGATAT GGAAGATCTG CTCTGCGTAA TTGGATGCTT 
    CAAGGATCTG TTGATGCAAA GCGATGGGAA AATGTGATTG TTCACACTGA CGATAAAGGA 
    CTCGGAGAAC CAGGATCTAC TGCTACTTGG CATGTTGGTG AAAAGGGAAC AACCGCATTC 
    AGATTCTTCA GAATTGCTCA AAACGGAAAG AACTCTTCGG GTCAAACACA TTACTTGTCT 
    TGTTCCGGAT TCGAGATTTA CGGAGACATC GTTGATGTGG TAACAGAAGC CATTTGCGAA 
    GACCCACCGA AAAAGGATTC ACCAGCCGGT ACGTCGTCAA CTCCTGGATC CTCATCATCA 
    GCCGCATTAC CTCCACTCAC AAAAGAACAA GTGCTTGAAA TGCTTCCAGC TCGTGAGAAC 
    AACAATCGTC TTAAATCAGG ACTATCGCTG GAGACTGTCA CTTCAATGCT GCAGAGGTCC 
    CGTCATCGTT CAAGAGGATC GTACAAAATA AGCGAGTCGA AGTCAAAAGT TGTACGTGGA 
    AAAGACTGGA GATGGGAAGA TCAGGATGGC GGAGAAGGAA AATTCGGTCG TATCACTAGT 
    CCGCCAGAAA GTGGCTGGGT TGATGTGACA TGGGACAATG GAAACGCCAA TTCATATCGA 
    TTCGGAGCCA ATGGAAACTT CGACATTGAG CGCGTTACCA GCACTGGACA TCGTTATTCA 
    ACACCATCGC TAGCTTCACA TGTTCCAACT TCGGTTATGG AGGCAGTTCG ACGAAATCGT 
    GCTTTCTACA CTCCAAAAAC AACTGGCGGT CCACCATCAT CATCCGTTTT TGGCACTTCA 
    TCATCAGCTG GATCTTCTCG TGGAGCTGCT TCTGCTCTCT CGCGATTTGC ATCAGTGAAA 
    AACACTACTC CAGCTGGAAC TCCATCATCG GGAGGATCGT CTGGAGGTGC AATCGGAAAG 
    AAATCAATGT CAACCACAAA TCTCGTCGAT GAGCGACAGA AGACATCTGG ACCATCCGTT 
    GCTTCAACAG GTCAAGCAGC TTCTGCTGAA TCTCTTCAAC ATCAAACTCC TTCTCTGGAG 
    AATCTTCTTG CAAGGGCCAT GCCACACGCA TTTGGACGTA TCGCAGAGAA TCAAGAACCC 
    GAAGATGAAC CAATGGGTGG AGAAGAGAGT GATTCGGCTG CAAGCATGAG ATCAGCAGCA 
    TCGTCAAATT CACAAATGTC TATGGGATCA TCTTCTCAAC AACAACAACA ACAAGATTCA 
    GACATGACTC CAAGGGACTC TGCGGGAACT CCTTCTACTC CTCGTGATGA TAAGAACCAA 
    ACACTCTCCG TTTCTGCACC TGATCTTGCT GCAGCACGGC AACGACAAGC TTCAGCCGAA 
    ACAGACGGAG ACGCAGATGC GGATGAGACA AACTCTGAAG ATAAAACTGT TGGTGCTGAT 
    GATGCAATGG AAGAAGACGA CGAAGAGGAG GAAACAATGG AGGACGAGGA AGATGATGAT 
    GACGACGATG ATGATGAAAG CTCCAACGAA AACCAAGAAA AGCTAGTCGA ATTGCTAGGT 
    GGAGAACGTG GCTTGTTTGA TAAACTTAAA GAAGTCATCA CCGGAGAATC ATTATCGGAC 
    GCTTCGTCGT CTGCCAAAGA CGCTACCACC AATGAAGCTC AGAAGAAGGG AGGCAAAAAA 
    CCTAAAAAGT GGTTCAAAAA GATGTCCAGC TACACGGATG TTCTTAAAGG TTTAATGCAA 
    AATCGTTATC CTGTCGCTCT TCTCGATCCA GCTGCTGCCG GTATTGAAAT GGATGAAATG 
    ATGGATGATG ATGACTACTA TGATTTCTCG GAAGATGGTC CAGATGATGG TGATTCAGTT 
    GAAGATGAAG TTGCTGCTCA CCTTGGAATG CCTGTTGACT CATTTGCATC AATGGTTGCT 
    GCCAGAACCC CAATAACATG GCGTCAATTT TCTGAATTAA TGAGTGGTTC TAATCGCGAA 
    CGTGCTGCTA TGGCAAGAGC TGTAGCATCG AGTCGAGGAT CCCCATGGGA AGATGATGCA 
    ACTGTCAAGT GTACATTCGA AGCCCTTATT CCAGCATTCG ATCCACGCCC AGGTCGTTCA 
    AATGTGAATC AAACTTTAGA AGTCGAACTT CCAACCGTCG TAAAAGATTT CGGTTCTACC 
    AAGGCTTCTT CTTCAAAGAT TGACAAAGAT GATCAAATGC GCTTCTTCCT TCGTGGACCA 
    AACATGTCAG GTGTCGATAA TGTGACCATC GAGATGAATG ATGATTCAGC ATCCCTGTTC 
    CGATACATGC AAATCATTAA CAACAGTGTC AATTGGGCTA CAAAAAGTGA TCGATCTCGC 
    CGGATCTGGG AGCCTACGTA CTCAATCTGT TACTGCTCTG CCGATCAAAC AAATGTCGAA 
    GTCTCCAAAA TTCCTGATGA GGAAAGTAGT ACACCATGTC AAGTAAATCA ATGTCTTGAA 
    ACCATCGGAT TGCTTTCAAG AATTCAACAG GCAATGCCAG AAGCAGAGAT AACTCCAAAT 
    GTGTTTATCA GTGATAAACT GACGTTGAAA GTAACTCAAG TTCTCTCTGA TGCTCTCGTT 
    GTCGCAGCAC GTTCTCTTCC TGAATGGTGC TCTCGTTTAG TCTATAAATA TCCATGTCTG 
    TTCACTGTGG AGACAAGAAA CATGTACATG CAGGCTACTG CATTCGGCGT GTCTCGTACA 
    ATCGTGTGGC TCCAACAACG TCGTGATGCT GCTGTCGAAC GTGCCCGCGG AAGTGCTCAA 
    GCTGGAAACA GTAGCGCTGC CCGGCAACAT GATCGTTATC ACGAATATCG GGTAGGGCGT 
    CTTCGCCATG AACGAGTTAA AGTGACTCGA GCTGAAGAGA CGCTTCTCGA TCAAGCGATC 
    CGATTAATGA AGTTCCATGC TGATCGTAAG GCGGTGCTAG AGATCGAATA CACCAATGAA 
    GAAGGAACAG GATTGGGACC AACTCTTGAG TTCTATGCAT TGGTTGCTGC AGAGTTGCAA 
    CGAAAGTCAT TGGCGTTGTG GGTTTGTGAT GATGATGATA CACATGCATC AAAGAGTGGA 
    GAAGAAAGAG AAGTCGATCT CGGTGAAGGA AAGAAGCCAA TTGGATACTA CGTTCGTCGC 
    GTTGGTGGTC TCTTCCCAGC ACCACTTCCA CCAGGAACCG ATGAAACAAA GAGAGCAGCA 
    GATATGTTCC GTGTTCTCGG AGTATTTCTT GCTAAAGTTC TTCTCGATGG TCGTCTTGTT 
    GATCTACCAC TATCCCGTCC ATTCCTCAAG TTACTCGTCC ATCCTCAGAT TGGAGATGAT 
    GCTCGTGGAC CAAACTTGCA CAAGATTCTC AGCCTAGACG ATTTCGAAGA AGTGAATCCA 
    GTAAAAGGAA GTTTCCTTAA AGAGTTGCGA GCTCTTGCTC AGCGTAAGCG ACTGATCGAG 
    AACGACACGA GCATCGATTC GAATTCCAAG AGAAGAAAAA TTGCGGAGCT CAAGTTACAC 
    ATTAAGGGAT CCACATGCAG AGTTGAAGAT CTTGCACTTA ATTTCACTGT CAATCCGCCA 
    TCAAAAGTGT TCCAATATGC CGAAATGGAG TTGGTTGACG GTGGATCCGA CATCGATGTT 
    ACAATCGACA ACGTGGAGCA ATATGTGGAG AAATGTGAAG AATTCTATTT GAACACAGGA 
    ATAGCCTACC AGATGCGTGC TTTCCGTGAT GGATTCGATC GCGTTTTTCC ACTTAGAACT 
    CTCAGAGCAT ACTCTCCAGA AGAAGTTCAG CGTCTTTTGT CCGGTGAACA GTGCCCAGAA 
    TGGTCTCGGG ACGATATTCT AAATTATACG GAGCCAAAAC TTGGATACAC TCGAGAATCT 
    CCTGGTTTCT TGAGATTTGT CGATGTAATG GAAGCATTGA CAGCTCAAGA ACGAAAGAAT 
    TTCCTTCAAT TCGCCACAGG ATGTTCGAGC TTGCCACCAG GTGGTCTTGC TAATCTTCAT 
    CCAAGGTTGA CAATTGTGCG AAAAGTGGAA AGTGGAGACG GCAGCTATCC ATCGGTTAAT 
    ACATGTGTGC ATTATCTCAA ACTCCCGGAG TACTCCTCGT CTGCAATCCT CCGCGAACGG 
    CTTCTCACGG CAATCAACGA GAAAGGATTC CATTTGAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306766.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293695.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.
    Price & Availability↑
    CloneID OCa342649
    Clone ID Related Accession (Same CDS sequence) NM_001306766.1 , NM_001306766.2
    Accession Version NM_001306766.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT 
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA 
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC 
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT 
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC 
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT 
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT 
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA 
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT 
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC 
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA 
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG 
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT 
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA 
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT 
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA 
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT 
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT 
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT 
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT 
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA 
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA 
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC 
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA 
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT 
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT 
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA 
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA 
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT 
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT 
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA 
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT 
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA 
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA 
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA 
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT 
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA 
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA 
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA 
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA 
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT 
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC 
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA 
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT 
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC 
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT 
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG 
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT 
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT 
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT 
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA 
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG 
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT 
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA 
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT 
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT 
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA 
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG 
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT 
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG 
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT 
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT 
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG 
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC 
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT 
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA 
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG 
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA 
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG 
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA 
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC 
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT 
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA 
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA 
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT 
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC 
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC 
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC 
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC 
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG 
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC 
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC 
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG 
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC 
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA 
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT 
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA 
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293695.1
    CDS1..7830
    Translation

    Target ORF information:

    RefSeq Version NM_001306766.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT 
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA 
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC 
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT 
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC 
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT 
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT 
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA 
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT 
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC 
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA 
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG 
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT 
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA 
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT 
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA 
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT 
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT 
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT 
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT 
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA 
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA 
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC 
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA 
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT 
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT 
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA 
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA 
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT 
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT 
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA 
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT 
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA 
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA 
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA 
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT 
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA 
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA 
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA 
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA 
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT 
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC 
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA 
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT 
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC 
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT 
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG 
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT 
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT 
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT 
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA 
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG 
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT 
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA 
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT 
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT 
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA 
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG 
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT 
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG 
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT 
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT 
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG 
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC 
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT 
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA 
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG 
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA 
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG 
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA 
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC 
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT 
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA 
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA 
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT 
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC 
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC 
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC 
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC 
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG 
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC 
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC 
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG 
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC 
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA 
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT 
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA 
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    hecd-1 ( NM_001306766.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293695.1 Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.
    Price & Availability↑
    CloneID OCa342649
    Clone ID Related Accession (Same CDS sequence) NM_001306766.1 , NM_001306766.2
    Accession Version NM_001306766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product E3 ubiquitin-protein ligase hecd-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306766.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT 
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA 
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC 
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT 
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC 
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT 
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT 
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA 
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT 
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC 
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA 
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG 
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT 
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA 
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT 
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA 
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT 
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT 
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT 
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT 
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA 
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA 
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC 
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA 
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT 
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT 
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA 
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA 
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT 
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT 
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA 
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT 
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA 
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA 
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA 
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT 
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA 
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA 
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA 
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA 
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT 
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC 
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA 
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT 
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC 
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT 
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG 
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT 
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT 
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT 
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA 
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG 
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT 
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA 
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT 
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT 
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA 
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG 
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT 
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG 
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT 
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT 
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG 
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC 
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT 
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA 
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG 
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA 
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG 
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA 
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC 
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT 
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA 
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA 
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT 
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC 
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC 
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC 
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC 
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG 
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC 
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC 
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG 
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC 
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA 
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT 
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA 
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293695.1
    CDS242..8071
    Translation

    Target ORF information:

    RefSeq Version NM_001306766.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans E3 ubiquitin-protein ligase hecd-1 (hecd-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001306766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGACGGAA TAGATCCGGA AACACTCCTC GAGTGGCTCC AAACGGGTAT TGGCGATGAA 
    CGGGATCTTC AATTAATGGC TCTTGAACAA CTATGCATGC TTCTTCTGAT GGCTGACAAT 
    ATTGATCGAT GCTTCGAATC ATGCCCACCA CGAACATTTA TTCCAGCACT ATGCAAAATT 
    TTCATCGATG AAACAGCACC TGATAATGTG CTCGAAGTAA CTGCTCGCGC AATCACTTAT 
    TATCTGGACG TGTCAAATGA ATGCACTCGT CGCATCACAC AAGTCGACGG AGCCGTGAAG 
    GCTATCTGCA CACGTCTTGC CGCTGCAGAC ATTTCAGATA GATCAAGTAA AGATCTGGCT 
    GAACAATGTG TCAAACTATT GGAACATGTG TGTCAACGTG AAACAATGGC AGTCTATGAT 
    GCAGGAGGAA TAAATGCAAT GCTCACTTTA GTTAGAGTAC ATGGAACACA AGTTCACAAA 
    GACACGATGC ATTCAGCAAT GTCAGTAGTG ACACGATTAT GTGGTAAAAT GGAACCAACT 
    GATCCAGAAT TGGGAAAATG TGCAGAAAGT CTTGGAGCTC TCCTTGAACA TGAAGACCCG 
    AAGGTGTCGG AAAGTGCTCT CAGATGTTTC GCCGCGCTTA CTGATCGCTT TGTTCGTAAA 
    ATGATGGATC CAGCAGAGCT AGCAATGCAT AGCAATCTTG TCGAACATTT GATATCAATT 
    ATGGTTGCTT CAAACGACGA AAACTCTCCG ACCACTGCTT CTGCCAATAT TCTCTCAATT 
    GTTCTTTCTT TGATTGGAAA TTTGTGCCGA GGTTCCAGCC TTATTACAGA AAAAGTGCTC 
    ACATCGCCTA ACATGATTAC TGGCCTCAAA GCTACTCTTA CAAACAAAGA AGAACGAGTG 
    GTAACAGATG GTCTTCGATT TTGTGATCTT CTTTTAGTTC TTCTTTGTGA AGGACGTAGT 
    GCTCTTCCCC TCACTTCAGT AGTATCAGGT GATTATGCAG CAGGTAGTGG AGCTGAACGT 
    GTTCATAGAC AATTGATTGA CGCCATCAGA CAGAAAGATT TGACTGCTTT GGTGGATGCC 
    ATCGAAAGTG GACAGGTTGA TGTGAATTTT GCTGATGATG TTGGACAATC ACTGACTAAT 
    TGGGCGTCTG CGTTTGGCTC GATCGAAATG CGTGGCGCCA ATCCTGATCT ACGAGACGAA 
    GATGGCAAGA CAGCTTTGGA CAAGGCACGA GAAAGATCTG ATGATGATCA CAATCAAGTC 
    GCCAACATTC TTGAATCGCC TAGCGCATTC ATGAGAAATA AAGAAGATCC GAAGGTGAAA 
    GCATCAACGT CGAAACAGCC AGGTACTTCA ACGAAACCTG AGCTTCCAAA TCCAAACCTT 
    GTTCGCAAAG TTTTGCATCA ACTTCTTCCA ATATTCTGTG AGATATTCCA AAAGTCTCTC 
    AACGGAAGTG TTCGTCGCAC ATCACTGTCT CTGATGCGAA AAATTGTGGA AAACATTGGA 
    GATCTTCGTC AAAGCGCCGT GGGAGATGGC AATGCTCCAG CTGCACAGTC CGCACGAAAA 
    ATGTCTACAG ATGTGTCTGC TGGTGCTGAA TCTTTGGTAG CTGTTGTCGT TTCTGTAATG 
    GATCAAGAAG ATGATCATGA GGGTCACGAA CAAGTGCTTC TTATTCTGGA ATCTCTTTTG 
    GAAAAAGATG CCGAACTCTG GGTTATTGAA TTGGTGCGAC TTGGTGTGTT TGAACGTGTC 
    GAGGCGATGG CAAAAGAGCC ACCAAAGGGA TTGGAGGAAG TTTTGAATGC AATTCATTTG 
    GAGGGAAGAT CTAGAGTTAC ACCCATGGAA ATTGACTTTG AGAATCAACC CTCGTCATCA 
    ACAGCTGTTC CTACGGCGAA CGACATCATG GATACCACTG TTCCATCTTC TTCTGGTGGA 
    GCCGACGCTG AATCAAACTC GAATCCTTCT ACAATTGAGA TGGCTGATCC AGAATCATCA 
    ACTCCTTCGT CATCCACACA GCAATCTATT TCTAAACCAA AAGCAACAGC CAGTTCAACA 
    GCATCTTCAG CTATTCTTCA AGTGGTATCC AAATTGAGTA GTGTTGCCAG TCTCGATAAA 
    TCAGCTGCCG CAGTTGATAA GAAACCAACA AAGACAGTAT TGAGCCAGGG AACACCTTAT 
    CGTTGGAAAG AATGGCGTAT CGTACGCGGA ACCACATCTC TATTCATCTG GAGTGATGTC 
    CTGCTCATTG AGTTACCATT CCAATCTAAT GGATGGTTCC GTTATTTAGC CGATAATGAC 
    TCACATGTAC AGTTCGTCAC TGGTACTGCA AGTGTTGATC AGCAAATGAC CGAGGAAGAA 
    AAGGATAATT TCCAAAAAAC CGAACGACGC GAAATGGTTT CCCGTTGGAA TGCAGTAAAA 
    GGAGTTTTCG ATGACGATTG GTCTTCTGTT CCAATTGCTG TTCTCGGAAT TCCCAGTAAC 
    GCTAAGAAGC TTGAAGTTCC GGCCTGGGAG CTATGGTCCT CCAAATCTTC TGAACTCCAA 
    ATTAAATCCA TTTCTTCATC GGCACCAACA GGACAAGCGA ACACTATGCT GACAACAATT 
    AAAATTCAGG ACGATGCTGG TGGTTTCCTT TTCGAAACTG GAACTGGACG AAAAACGAAT 
    GTTATGCCAG AGCATGCATT ACCATCGGAT TTCCATACTG GATGGAGTTC ACACGGAGTA 
    TCAACCCGAA AAATGAAGTT CCGTCAAGAT ATTCAGAAGC GTAAAGTTCA AGAATTGGCC 
    TGGAAATTGT GGAACGATCA TCTGAAAGAG GCCCATGCGA AGCCGAGAGA GGCACTTGTT 
    AGATTGGAGA ATGCAGCACG TACCATAGAG AGCACTATTC GACACGTTAA AGCTCAGAGC 
    AACTTCAAAC ATCGGAATGT GAAGCAACCA AGAATTGAAA GAGTACAAGA ATATTGTGCT 
    GCCATCAGTA CACTTCATGA GTCAATCGTT GATGATCGTC GTTTGTCTAC ATTCGAATTT 
    TCTGTTTCGG GAATTGTGCC TGCGCTTTTT GGCCTTTTAT CAATGATGGA AAAGTTTCCA 
    GACAGTTTCC CATCAAGGAT CTTCAAGGAA CAATTCTCAA AAGGAGAAGC ACTTTCATAT 
    CTTGCACTGA AAATAGTTGC TGTTTTGGAA GCGAATGAAA AGTTCCCACA GCATTTATAC 
    GATAGTCCTG GAGGTTCTTC CTTCGGCCTT CAACTTCTGT CTCGTCGTGT GCGCACAAAA 
    CTCGAAATGC TTCCACGTGC TGATGGAAAG GAGAATAACG ACGAAAATCT TGTGAACAAG 
    ACAGGAAAGA TAGTAAAGTG CGAACCTCTT GCATCTGTGG GAGCCATCAG AGCGTATCTT 
    CATAGAATGG TTACTCGACA GTGGCATGAT CGTGAACGAG CCAATTATAG ATATGTCAAA 
    GAGATTCAAG AGTTGAAGAC AAAAGGAAAA TCTATTGAAT TGAGACATGT TTCTGACTTT 
    GATGAGAATG GTGTTATTTA TTGGATCGGA ACAAATGGAA GAGCTGCACC ACTATGGACA 
    AATCCAGCAA CCGTAAAAGC AGTTAAAATC ACTTGTTCGG ATACTCGCCA ACCATTCGGT 
    AAACCTGAAG ATCTTCTGTC ACGTGATCAA AATCCTATCA ATTGTCATAC TTCCGATGAT 
    AAAAATGCTC ATTTCACAAT TGATTTGGGT CTGTTCGTTG TTCCAACTTC TTATTCTCTT 
    CGTCACTCGC GTGGATATGG AAGATCTGCT CTGCGTAATT GGATGCTTCA AGGATCTGTT 
    GATGCAAAGC GATGGGAAAA TGTGATTGTT CACACTGACG ATAAAGGACT CGGAGAACCA 
    GGATCTACTG CTACTTGGCA TGTTGGTGAA AAGGGAACAA CCGCATTCAG ATTCTTCAGA 
    ATTGCTCAAA ACGGAAAGAA CTCTTCGGGT CAAACACATT ACTTGTCTTG TTCCGGATTC 
    GAGATTTACG GAGACATCGT TGATGTGGTA ACAGAAGCCA TTTGCGAAGA CCCACCGAAA 
    AAGGATTCAC CAGCCGGTAC GTCGTCAACT CCTGGATCCT CATCATCAGC CGCATTACCT 
    CCACTCACAA AAGAACAAGT GCTTGAAATG CTTCCAGCTC GTGAGAACAA CAATCGTCTT 
    AAATCAGGAC TATCGCTGGA GACTGTCACT TCAATGCTGC AGAGGTCCCG TCATCGTTCA 
    AGAGGATCGT ACAAAATAAG CGAGTCGAAG TCAAAAGTTG TACGTGGAAA AGACTGGAGA 
    TGGGAAGATC AGGATGGCGG AGAAGGAAAA TTCGGTCGTA TCACTAGTCC GCCAGAAAGT 
    GGCTGGGTTG ATGTGACATG GGACAATGGA AACGCCAATT CATATCGATT CGGAGCCAAT 
    GGAAACTTCG ACATTGAGCG CGTTACCAGC ACTGGACATC GTTATTCAAC ACCATCGCTA 
    GCTTCACATG TTCCAACTTC GGTTATGGAG GCAGTTCGAC GAAATCGTGC TTTCTACACT 
    CCAAAAACAA CTGGCGGTCC ACCATCATCA TCCGTTTTTG GCACTTCATC ATCAGCTGGA 
    TCTTCTCGTG GAGCTGCTTC TGCTCTCTCG CGATTTGCAT CAGTGAAAAA CACTACTCCA 
    GCTGGAACTC CATCATCGGG AGGATCGTCT GGAGGTGCAA TCGGAAAGAA ATCAATGTCA 
    ACCACAAATC TCGTCGATGA GCGACAGAAG ACATCTGGAC CATCCGTTGC TTCAACAGGT 
    CAAGCAGCTT CTGCTGAATC TCTTCAACAT CAAACTCCTT CTCTGGAGAA TCTTCTTGCA 
    AGGGCCATGC CACACGCATT TGGACGTATC GCAGAGAATC AAGAACCCGA AGATGAACCA 
    ATGGGTGGAG AAGAGAGTGA TTCGGCTGCA AGCATGAGAT CAGCAGCATC GTCAAATTCA 
    CAAATGTCTA TGGGATCATC TTCTCAACAA CAACAACAAC AAGATTCAGA CATGACTCCA 
    AGGGACTCTG CGGGAACTCC TTCTACTCCT CGTGATGATA AGAACCAAAC ACTCTCCGTT 
    TCTGCACCTG ATCTTGCTGC AGCACGGCAA CGACAAGCTT CAGCCGAAAC AGACGGAGAC 
    GCAGATGCGG ATGAGACAAA CTCTGAAGAT AAAACTGTTG GTGCTGATGA TGCAATGGAA 
    GAAGACGACG AAGAGGAGGA AACAATGGAG GACGAGGAAG ATGATGATGA CGACGATGAT 
    GATGAAAGCT CCAACGAAAA CCAAGAAAAG CTAGTCGAAT TGCTAGGTGG AGAACGTGGC 
    TTGTTTGATA AACTTAAAGA AGTCATCACC GGAGAATCAT TATCGGACGC TTCGTCGTCT 
    GCCAAAGACG CTACCACCAA TGAAGCTCAG AAGAAGGGAG GCAAAAAACC TAAAAAGTGG 
    TTCAAAAAGA TGTCCAGCTA CACGGATGTT CTTAAAGGTT TAATGCAAAA TCGTTATCCT 
    GTCGCTCTTC TCGATCCAGC TGCTGCCGGT ATTGAAATGG ATGAAATGAT GGATGATGAT 
    GACTACTATG ATTTCTCGGA AGATGGTCCA GATGATGGTG ATTCAGTTGA AGATGAAGTT 
    GCTGCTCACC TTGGAATGCC TGTTGACTCA TTTGCATCAA TGGTTGCTGC CAGAACCCCA 
    ATAACATGGC GTCAATTTTC TGAATTAATG AGTGGTTCTA ATCGCGAACG TGCTGCTATG 
    GCAAGAGCTG TAGCATCGAG TCGAGGATCC CCATGGGAAG ATGATGCAAC TGTCAAGTGT 
    ACATTCGAAG CCCTTATTCC AGCATTCGAT CCACGCCCAG GTCGTTCAAA TGTGAATCAA 
    ACTTTAGAAG TCGAACTTCC AACCGTCGTA AAAGATTTCG GTTCTACCAA GGCTTCTTCT 
    TCAAAGATTG ACAAAGATGA TCAAATGCGC TTCTTCCTTC GTGGACCAAA CATGTCAGGT 
    GTCGATAATG TGACCATCGA GATGAATGAT GATTCAGCAT CCCTGTTCCG ATACATGCAA 
    ATCATTAACA ACAGTGTCAA TTGGGCTACA AAAAGTGATC GATCTCGCCG GATCTGGGAG 
    CCTACGTACT CAATCTGTTA CTGCTCTGCC GATCAAACAA ATGTCGAAGT CTCCAAAATT 
    CCTGATGAGG AAAGTAGTAC ACCATGTCAA GTAAATCAAT GTCTTGAAAC CATCGGATTG 
    CTTTCAAGAA TTCAACAGGC AATGCCAGAA GCAGAGATAA CTCCAAATGT GTTTATCAGT 
    GATAAACTGA CGTTGAAAGT AACTCAAGTT CTCTCTGATG CTCTCGTTGT CGCAGCACGT 
    TCTCTTCCTG AATGGTGCTC TCGTTTAGTC TATAAATATC CATGTCTGTT CACTGTGGAG 
    ACAAGAAACA TGTACATGCA GGCTACTGCA TTCGGCGTGT CTCGTACAAT CGTGTGGCTC 
    CAACAACGTC GTGATGCTGC TGTCGAACGT GCCCGCGGAA GTGCTCAAGC TGGAAACAGT 
    AGCGCTGCCC GGCAACATGA TCGTTATCAC GAATATCGGG TAGGGCGTCT TCGCCATGAA 
    CGAGTTAAAG TGACTCGAGC TGAAGAGACG CTTCTCGATC AAGCGATCCG ATTAATGAAG 
    TTCCATGCTG ATCGTAAGGC GGTGCTAGAG ATCGAATACA CCAATGAAGA AGGAACAGGA 
    TTGGGACCAA CTCTTGAGTT CTATGCATTG GTTGCTGCAG AGTTGCAACG AAAGTCATTG 
    GCGTTGTGGG TTTGTGATGA TGATGATACA CATGCATCAA AGAGTGGAGA AGAAAGAGAA 
    GTCGATCTCG GTGAAGGAAA GAAGCCAATT GGATACTACG TTCGTCGCGT TGGTGGTCTC 
    TTCCCAGCAC CACTTCCACC AGGAACCGAT GAAACAAAGA GAGCAGCAGA TATGTTCCGT 
    GTTCTCGGAG TATTTCTTGC TAAAGTTCTT CTCGATGGTC GTCTTGTTGA TCTACCACTA 
    TCCCGTCCAT TCCTCAAGTT ACTCGTCCAT CCTCAGATTG GAGATGATGC TCGTGGACCA 
    AACTTGCACA AGATTCTCAG CCTAGACGAT TTCGAAGAAG TGAATCCAGT AAAAGGAAGT 
    TTCCTTAAAG AGTTGCGAGC TCTTGCTCAG CGTAAGCGAC TGATCGAGAA CGACACGAGC 
    ATCGATTCGA ATTCCAAGAG AAGAAAAATT GCGGAGCTCA AGTTACACAT TAAGGGATCC 
    ACATGCAGAG TTGAAGATCT TGCACTTAAT TTCACTGTCA ATCCGCCATC AAAAGTGTTC 
    CAATATGCCG AAATGGAGTT GGTTGACGGT GGATCCGACA TCGATGTTAC AATCGACAAC 
    GTGGAGCAAT ATGTGGAGAA ATGTGAAGAA TTCTATTTGA ACACAGGAAT AGCCTACCAG 
    ATGCGTGCTT TCCGTGATGG ATTCGATCGC GTTTTTCCAC TTAGAACTCT CAGAGCATAC 
    TCTCCAGAAG AAGTTCAGCG TCTTTTGTCC GGTGAACAGT GCCCAGAATG GTCTCGGGAC 
    GATATTCTAA ATTATACGGA GCCAAAACTT GGATACACTC GAGAATCTCC TGGTTTCTTG 
    AGATTTGTCG ATGTAATGGA AGCATTGACA GCTCAAGAAC GAAAGAATTT CCTTCAATTC 
    GCCACAGGAT GTTCGAGCTT GCCACCAGGT GGTCTTGCTA ATCTTCATCC AAGGTTGACA 
    ATTGTGCGAA AAGTGGAAAG TGGAGACGGC AGCTATCCAT CGGTTAATAC ATGTGTGCAT 
    TATCTCAAAC TCCCGGAGTA CTCCTCGTCT GCAATCCTCC GCGAACGGCT TCTCACGGCA 
    ATCAACGAGA AAGGATTCCA TTTGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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