lag-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	lag-1
Entrez Gene ID	177373
Full Name	Lin-12 And Glp-1 phenotype
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_171350.4	NP_741410.1	Lin-12 And Glp-1 phenotype
NM_001028107.3	NP_001023278.1	Lin-12 And Glp-1 phenotype
NM_001306810.1	NP_001293739.1	Lin-12 And Glp-1 phenotype
NM_171350.5	NP_741410.1	Lin-12 And Glp-1 phenotype
NM_001372835.1	NP_001360306.1	Lin-12 And Glp-1 phenotype
NM_001306811.1	NP_001293740.1	Lin-12 And Glp-1 phenotype
NM_001028107.4	NP_001023278.1	Lin-12 And Glp-1 phenotype
NM_001306811.2	NP_001293740.1	Lin-12 And Glp-1 phenotype

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RBPJ	XP_004936192.1
Danio rerio (zebrafish)	rbpjb	NP_001077020.1
Caenorhabditis elegans (roundworm)	lag-1	NP_001023278.1
	RBPJ	XP_001084065.1
Bos taurus (cattle)	RBPJ	NP_001070469.1
Mus musculus (house mouse)	Rbpj	NP_001074396.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008935	XP_319690.4
Homo sapiens (human)	RBPJ	NP_056958.3
Drosophila melanogaster (fruit fly)	Su(H)	NP_476868.1
Pan troglodytes (chimpanzee)	RBPJ	XP_517135.2
Canis lupus familiaris (dog)	LOC479122	XP_005618707.1
Rattus norvegicus (Norway rat)	Rbpj	NP_001100101.1
Xenopus tropicalis (tropical clawed frog)	LOC100491789	XP_002939143.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LIN-12 signaling is necessary for the suppression of lag-1 expression in the anchor cell in Caenorhabditis elegans.
Title: LIN-12/Notch regulates lag-1 and lin-12 expression during anchor cell/ventral uterine precursor cell fate specification.

Regulation of hlh-6 by LAG-1 is strictly negative: removal of HRL1 or LAG-1 regulation results in ectopic expression of hlh-6, but does not affect expression in pharyngeal glands.
Title: The CSL transcription factor LAG-1 directly represses hlh-6 expression in C. elegans.

2.85 A crystal structure of CSL ortholog Lag-1 with a target DNA
Title: Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA.

The following lag-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lag-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342672	NM_001306810.1 Latest version!	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa342672	NM_001372835.1 Latest version!	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa342673	NM_001306811.2 Latest version!	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa344078	NM_171350.5 Latest version!	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa344079	NM_001028107.4 Latest version!	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa344078	NM_171350.4	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa344079	NM_001028107.3	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa342673	NM_001306811.1	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lag-1 ( NM_001306810.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293739.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Price & Availability↑

CloneID	OCa342672
Clone ID Related Accession (Same CDS sequence)	NM_001306810.1 , NM_001372835.1
Accession Version	NM_001306810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTTCCAGT CGGTATCGCC GCTGGCCGGT GTCGCTGCAG CGCCGAGCCA AATTGCCGCA 
CTTCAACAGA TACAAGCGTT GATGACATTT CAAATGCAGC AGAATAATTT ATTTCCCAAA 
ATTGACACTA TATCGAAATC GCCAACTCCA GAACTCGCAT CTCCATCGGC AAAACGAATG 
AGACTTTCTC CCAGTACGAG TAGCCACAGT GATGTCGCAT CAACTTCGAA AGGAACAAAT 
GGGCAAGATC AGTCGAAAAA TTCGCCGACA AATTCTGATC CATTCTACAC AATCGGGCAG 
TCAACTCCAA ACACATCATC ATTCCTGGAC ACTTCAAATT CAAGTTTTGG AGCTCCGTCA 
ACAGTGGTTG CCAATCCGAT GACCAACTAT CAATTGGCAT TTCAAGCGAA ACTCGGAAGT 
CTTCACAGTT TGATTGGAGA TTCTGTTCAA TCGTTAACAA GCGATCGAAT GATTGATTTC 
TTGTCAAACA AGGAGAAGTA CGAATGCGTC ATATCGATTT TCCATGCCAA AGTTGCCCAA 
AAGTCATATG GAAATGAGAA AAGATTCTTC TGCCCACCAC CATGCATTTA TCTTATTGGC 
CAAGGCTGGA AACTCAAAAA GGATCGTGTC GCTCAACTGT ACAAAACACT CAAAGCTTCA 
GCTCAAAAGG ATGCTGCAAT CGAGAATGAT CCGATTCACG AGCAACAAGC CACAGAACTC 
GTCGCCTACA TTGGAATCGG ATCAGACACT TCCGAGCGAC AACAACTTGA TTTTTCAACG 
GGAAAAGTTC GACATCCTGG AGATCAGAGG CAGGATCCAA ATATTTATGA TTATTGTGCC 
GCGAAGACTC TGTATATTAG TGATTCGGAC AAGAGAAAAT ATTTCGATTT AAATGCTCAA 
TTCTTCTATG GATGTGGAAT GGAAATTGGA GGATTCGTAT CACAAAGAAT CAAAGTAATC 
TCAAAACCAT CGAAAAAGAA GCAAAGTATG AAAAATACGG ACTGCAAGTA TCTTTGTATT 
GCCAGTGGAA CCAAAGTTGC TCTATTCAAT CGTCTCCGGT CACAGACAGT CAGTACAAGA 
TACCTTCATG TTGAGGGAAA TGCATTCCAT GCTTCCTCCA CGAAATGGGG AGCCTTTACT 
ATTCATCTTT TTGACGACGA ACGTGGTTTG CAAGAGACTG ATAATTTTGC AGTTCGTGAT 
GGATTTGTTT ACTATGGAAG TGTCGTTAAG CTTGTTGATA GTGTTACCGG AATCGCACTG 
CCAAGATTGA GAATCAGAAA GGTCGATAAG CAGCAAGTAA TTCTTGATGC ATCGTGTAGT 
GAAGAACCAG TCTCTCAACT TCACAAATGT GCATTCCAAA TGATTGACAA TGAGCTCGTC 
TATCTTTGTC TCAGTCATGA CAAGATTATT CAACATCAAG CCACAGCTAT CAATGAGCAT 
CGTCATCAGA TCAACGATGG AGCTGCGTGG ACAATCATCT CGACTGATAA GGCTGAATAT 
CGATTCTTCG AAGCGATGGG ACAGGTTGCG AACCCGATTT CTCCATGTCC AGTTGTCGGT 
TCACTTGAAG TTGATGGACA TGGTGAAGCG TCGCGTGTCG AGTTGCATGG AAGAGATTTT 
AAGCCAAATC TGAAAGTTTG GTTTGGAGCA ACCCCGGTTG AAACGACGTT CAGAAGTGAA 
GAGAGTCTTC ATTGTTCGAT ACCACCTGTG AGTCAGGTTC GAAATGAGCA AACTCACTGG 
ATGTTTACGA ATAGGACAAC CGGCGATGTT GAGGTTCCAA TTTCATTGGT ACGAGATGAT 
GGTGTCGTCT ACTCGTCGGG TCTCACATTT TCCTACAAAT CATTGGAACG ACATGGACCG 
TGCAGAATTG TGTCCAATTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293739.1
CDS	1..1884
Translation	MFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001306810.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306810.1

ORF Insert Sequence:

ATGTTCCAGT CGGTATCGCC GCTGGCCGGT GTCGCTGCAG CGCCGAGCCA AATTGCCGCA 
CTTCAACAGA TACAAGCGTT GATGACATTT CAAATGCAGC AGAATAATTT ATTTCCCAAA 
ATTGACACTA TATCGAAATC GCCAACTCCA GAACTCGCAT CTCCATCGGC AAAACGAATG 
AGACTTTCTC CCAGTACGAG TAGCCACAGT GATGTCGCAT CAACTTCGAA AGGAACAAAT 
GGGCAAGATC AGTCGAAAAA TTCGCCGACA AATTCTGATC CATTCTACAC AATCGGGCAG 
TCAACTCCAA ACACATCATC ATTCCTGGAC ACTTCAAATT CAAGTTTTGG AGCTCCGTCA 
ACAGTGGTTG CCAATCCGAT GACCAACTAT CAATTGGCAT TTCAAGCGAA ACTCGGAAGT 
CTTCACAGTT TGATTGGAGA TTCTGTTCAA TCGTTAACAA GCGATCGAAT GATTGATTTC 
TTGTCAAACA AGGAGAAGTA CGAATGCGTC ATATCGATTT TCCATGCCAA AGTTGCCCAA 
AAGTCATATG GAAATGAGAA AAGATTCTTC TGCCCACCAC CATGCATTTA TCTTATTGGC 
CAAGGCTGGA AACTCAAAAA GGATCGTGTC GCTCAACTGT ACAAAACACT CAAAGCTTCA 
GCTCAAAAGG ATGCTGCAAT CGAGAATGAT CCGATTCACG AGCAACAAGC CACAGAACTC 
GTCGCCTACA TTGGAATCGG ATCAGACACT TCCGAGCGAC AACAACTTGA TTTTTCAACG 
GGAAAAGTTC GACATCCTGG AGATCAGAGG CAGGATCCAA ATATTTATGA TTATTGTGCC 
GCGAAGACTC TGTATATTAG TGATTCGGAC AAGAGAAAAT ATTTCGATTT AAATGCTCAA 
TTCTTCTATG GATGTGGAAT GGAAATTGGA GGATTCGTAT CACAAAGAAT CAAAGTAATC 
TCAAAACCAT CGAAAAAGAA GCAAAGTATG AAAAATACGG ACTGCAAGTA TCTTTGTATT 
GCCAGTGGAA CCAAAGTTGC TCTATTCAAT CGTCTCCGGT CACAGACAGT CAGTACAAGA 
TACCTTCATG TTGAGGGAAA TGCATTCCAT GCTTCCTCCA CGAAATGGGG AGCCTTTACT 
ATTCATCTTT TTGACGACGA ACGTGGTTTG CAAGAGACTG ATAATTTTGC AGTTCGTGAT 
GGATTTGTTT ACTATGGAAG TGTCGTTAAG CTTGTTGATA GTGTTACCGG AATCGCACTG 
CCAAGATTGA GAATCAGAAA GGTCGATAAG CAGCAAGTAA TTCTTGATGC ATCGTGTAGT 
GAAGAACCAG TCTCTCAACT TCACAAATGT GCATTCCAAA TGATTGACAA TGAGCTCGTC 
TATCTTTGTC TCAGTCATGA CAAGATTATT CAACATCAAG CCACAGCTAT CAATGAGCAT 
CGTCATCAGA TCAACGATGG AGCTGCGTGG ACAATCATCT CGACTGATAA GGCTGAATAT 
CGATTCTTCG AAGCGATGGG ACAGGTTGCG AACCCGATTT CTCCATGTCC AGTTGTCGGT 
TCACTTGAAG TTGATGGACA TGGTGAAGCG TCGCGTGTCG AGTTGCATGG AAGAGATTTT 
AAGCCAAATC TGAAAGTTTG GTTTGGAGCA ACCCCGGTTG AAACGACGTT CAGAAGTGAA 
GAGAGTCTTC ATTGTTCGAT ACCACCTGTG AGTCAGGTTC GAAATGAGCA AACTCACTGG 
ATGTTTACGA ATAGGACAAC CGGCGATGTT GAGGTTCCAA TTTCATTGGT ACGAGATGAT 
GGTGTCGTCT ACTCGTCGGG TCTCACATTT TCCTACAAAT CATTGGAACG ACATGGACCG 
TGCAGAATTG TGTCCAATTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_001372835.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360306.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

Price & Availability↑

CloneID	OCa342672
Clone ID Related Accession (Same CDS sequence)	NM_001306810.1 , NM_001372835.1
Accession Version	NM_001372835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282).

ATGTTCCAGT CGGTATCGCC GCTGGCCGGT GTCGCTGCAG CGCCGAGCCA AATTGCCGCA 
CTTCAACAGA TACAAGCGTT GATGACATTT CAAATGCAGC AGAATAATTT ATTTCCCAAA 
ATTGACACTA TATCGAAATC GCCAACTCCA GAACTCGCAT CTCCATCGGC AAAACGAATG 
AGACTTTCTC CCAGTACGAG TAGCCACAGT GATGTCGCAT CAACTTCGAA AGGAACAAAT 
GGGCAAGATC AGTCGAAAAA TTCGCCGACA AATTCTGATC CATTCTACAC AATCGGGCAG 
TCAACTCCAA ACACATCATC ATTCCTGGAC ACTTCAAATT CAAGTTTTGG AGCTCCGTCA 
ACAGTGGTTG CCAATCCGAT GACCAACTAT CAATTGGCAT TTCAAGCGAA ACTCGGAAGT 
CTTCACAGTT TGATTGGAGA TTCTGTTCAA TCGTTAACAA GCGATCGAAT GATTGATTTC 
TTGTCAAACA AGGAGAAGTA CGAATGCGTC ATATCGATTT TCCATGCCAA AGTTGCCCAA 
AAGTCATATG GAAATGAGAA AAGATTCTTC TGCCCACCAC CATGCATTTA TCTTATTGGC 
CAAGGCTGGA AACTCAAAAA GGATCGTGTC GCTCAACTGT ACAAAACACT CAAAGCTTCA 
GCTCAAAAGG ATGCTGCAAT CGAGAATGAT CCGATTCACG AGCAACAAGC CACAGAACTC 
GTCGCCTACA TTGGAATCGG ATCAGACACT TCCGAGCGAC AACAACTTGA TTTTTCAACG 
GGAAAAGTTC GACATCCTGG AGATCAGAGG CAGGATCCAA ATATTTATGA TTATTGTGCC 
GCGAAGACTC TGTATATTAG TGATTCGGAC AAGAGAAAAT ATTTCGATTT AAATGCTCAA 
TTCTTCTATG GATGTGGAAT GGAAATTGGA GGATTCGTAT CACAAAGAAT CAAAGTAATC 
TCAAAACCAT CGAAAAAGAA GCAAAGTATG AAAAATACGG ACTGCAAGTA TCTTTGTATT 
GCCAGTGGAA CCAAAGTTGC TCTATTCAAT CGTCTCCGGT CACAGACAGT CAGTACAAGA 
TACCTTCATG TTGAGGGAAA TGCATTCCAT GCTTCCTCCA CGAAATGGGG AGCCTTTACT 
ATTCATCTTT TTGACGACGA ACGTGGTTTG CAAGAGACTG ATAATTTTGC AGTTCGTGAT 
GGATTTGTTT ACTATGGAAG TGTCGTTAAG CTTGTTGATA GTGTTACCGG AATCGCACTG 
CCAAGATTGA GAATCAGAAA GGTCGATAAG CAGCAAGTAA TTCTTGATGC ATCGTGTAGT 
GAAGAACCAG TCTCTCAACT TCACAAATGT GCATTCCAAA TGATTGACAA TGAGCTCGTC 
TATCTTTGTC TCAGTCATGA CAAGATTATT CAACATCAAG CCACAGCTAT CAATGAGCAT 
CGTCATCAGA TCAACGATGG AGCTGCGTGG ACAATCATCT CGACTGATAA GGCTGAATAT 
CGATTCTTCG AAGCGATGGG ACAGGTTGCG AACCCGATTT CTCCATGTCC AGTTGTCGGT 
TCACTTGAAG TTGATGGACA TGGTGAAGCG TCGCGTGTCG AGTTGCATGG AAGAGATTTT 
AAGCCAAATC TGAAAGTTTG GTTTGGAGCA ACCCCGGTTG AAACGACGTT CAGAAGTGAA 
GAGAGTCTTC ATTGTTCGAT ACCACCTGTG AGTCAGGTTC GAAATGAGCA AACTCACTGG 
ATGTTTACGA ATAGGACAAC CGGCGATGTT GAGGTTCCAA TTTCATTGGT ACGAGATGAT 
GGTGTCGTCT ACTCGTCGGG TCTCACATTT TCCTACAAAT CATTGGAACG ACATGGACCG 
TGCAGAATTG TGTCCAATTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360306.1
CDS	138..2021
Translation	MFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001372835.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372835.1

ORF Insert Sequence:

ATGTTCCAGT CGGTATCGCC GCTGGCCGGT GTCGCTGCAG CGCCGAGCCA AATTGCCGCA 
CTTCAACAGA TACAAGCGTT GATGACATTT CAAATGCAGC AGAATAATTT ATTTCCCAAA 
ATTGACACTA TATCGAAATC GCCAACTCCA GAACTCGCAT CTCCATCGGC AAAACGAATG 
AGACTTTCTC CCAGTACGAG TAGCCACAGT GATGTCGCAT CAACTTCGAA AGGAACAAAT 
GGGCAAGATC AGTCGAAAAA TTCGCCGACA AATTCTGATC CATTCTACAC AATCGGGCAG 
TCAACTCCAA ACACATCATC ATTCCTGGAC ACTTCAAATT CAAGTTTTGG AGCTCCGTCA 
ACAGTGGTTG CCAATCCGAT GACCAACTAT CAATTGGCAT TTCAAGCGAA ACTCGGAAGT 
CTTCACAGTT TGATTGGAGA TTCTGTTCAA TCGTTAACAA GCGATCGAAT GATTGATTTC 
TTGTCAAACA AGGAGAAGTA CGAATGCGTC ATATCGATTT TCCATGCCAA AGTTGCCCAA 
AAGTCATATG GAAATGAGAA AAGATTCTTC TGCCCACCAC CATGCATTTA TCTTATTGGC 
CAAGGCTGGA AACTCAAAAA GGATCGTGTC GCTCAACTGT ACAAAACACT CAAAGCTTCA 
GCTCAAAAGG ATGCTGCAAT CGAGAATGAT CCGATTCACG AGCAACAAGC CACAGAACTC 
GTCGCCTACA TTGGAATCGG ATCAGACACT TCCGAGCGAC AACAACTTGA TTTTTCAACG 
GGAAAAGTTC GACATCCTGG AGATCAGAGG CAGGATCCAA ATATTTATGA TTATTGTGCC 
GCGAAGACTC TGTATATTAG TGATTCGGAC AAGAGAAAAT ATTTCGATTT AAATGCTCAA 
TTCTTCTATG GATGTGGAAT GGAAATTGGA GGATTCGTAT CACAAAGAAT CAAAGTAATC 
TCAAAACCAT CGAAAAAGAA GCAAAGTATG AAAAATACGG ACTGCAAGTA TCTTTGTATT 
GCCAGTGGAA CCAAAGTTGC TCTATTCAAT CGTCTCCGGT CACAGACAGT CAGTACAAGA 
TACCTTCATG TTGAGGGAAA TGCATTCCAT GCTTCCTCCA CGAAATGGGG AGCCTTTACT 
ATTCATCTTT TTGACGACGA ACGTGGTTTG CAAGAGACTG ATAATTTTGC AGTTCGTGAT 
GGATTTGTTT ACTATGGAAG TGTCGTTAAG CTTGTTGATA GTGTTACCGG AATCGCACTG 
CCAAGATTGA GAATCAGAAA GGTCGATAAG CAGCAAGTAA TTCTTGATGC ATCGTGTAGT 
GAAGAACCAG TCTCTCAACT TCACAAATGT GCATTCCAAA TGATTGACAA TGAGCTCGTC 
TATCTTTGTC TCAGTCATGA CAAGATTATT CAACATCAAG CCACAGCTAT CAATGAGCAT 
CGTCATCAGA TCAACGATGG AGCTGCGTGG ACAATCATCT CGACTGATAA GGCTGAATAT 
CGATTCTTCG AAGCGATGGG ACAGGTTGCG AACCCGATTT CTCCATGTCC AGTTGTCGGT 
TCACTTGAAG TTGATGGACA TGGTGAAGCG TCGCGTGTCG AGTTGCATGG AAGAGATTTT 
AAGCCAAATC TGAAAGTTTG GTTTGGAGCA ACCCCGGTTG AAACGACGTT CAGAAGTGAA 
GAGAGTCTTC ATTGTTCGAT ACCACCTGTG AGTCAGGTTC GAAATGAGCA AACTCACTGG 
ATGTTTACGA ATAGGACAAC CGGCGATGTT GAGGTTCCAA TTTCATTGGT ACGAGATGAT 
GGTGTCGTCT ACTCGTCGGG TCTCACATTT TCCTACAAAT CATTGGAACG ACATGGACCG 
TGCAGAATTG TGTCCAATTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_001306811.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293740.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Price & Availability↑

CloneID	OCa342673
Clone ID Related Accession (Same CDS sequence)	NM_001306811.1 , NM_001306811.2
Accession Version	NM_001306811.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTTTTCGT GGAGGCGTGG CGATTGTGAG TCGCAGAAGG AAGAAAACCG AAGTGAGGAA 
AGGAAAGGAG AGGAGACTAT TCGTTTTCCC ACCCGCTCTC CTTTTCATTG TGTGCTCTTC 
TTGCTGACAG ACGGCTTCGT TCTTCACAAA CCCACAGCCA GCCCCACCGT CGGTTTTTCC 
CCCACAATAT TCAGCTTCTA CTATTCTCGG TGGGAATCTT CGCATCGGAT ATCCCACGAC 
GAGAGCGGCT TCTGCACCGC GAAGACCCCC CTTCAAGACT CGACTTTCAC GCGCCACCCA 
TCCACATCGG TCCCATCGTC GCCCTCAACG CCAAGACACT CCGGGATGGA TTATCATCAG 
TCGTCTTCGG TAGCTTCTTC CGAGTCGACA GCGTCTACAG TAGCCGCGGC GGCGGCTGCT 
GCAGCTGCTG CATCACTCAA TCAGCATCAT CATCCACATC TATATTGTGA TGATGGACTT 
CTATCGAGAA GTTTAACTGA TATGGTATCA TCTGGTGGAT ATGATAGCTC ATCATCTTCT 
CTAAGTGCAG CTGCATCAAT GTGTTATCCG ACTCCTGATG CATATTACTA TCATGCTCCA 
CCACCACCAC CTCCTCCACA AGCACAACAG GGATTCAGTT CAACAGACGC GTGGCTTCAA 
ATGCAAATGC AACCCACGTA TCATAACTTT GGCAGTACTG TCGTCTCTAC CAATTCCCCA 
CTTCCAAGCC ACCTTCTCAG TTATGGAGGA CAACCAGCAT TTGCTGATCC ATTCTACACA 
ATCGGGCAGT CAACTCCAAA CACATCATCA TTCCTGGACA CTTCAAATTC AAGTTTTGGA 
GCTCCGTCAA CAGTGGTTGC CAATCCGATG ACCAACTATC AATTGGCATT TCAAGCGAAA 
CTCGGAAGTC TTCACAGTTT GATTGGAGAT TCTGTTCAAT CGTTAACAAG CGATCGAATG 
ATTGATTTCT TGTCAAACAA GGAGAAGTAC GAATGCGTCA TATCGATTTT CCATGCCAAA 
GTTGCCCAAA AGTCATATGG AAATGAGAAA AGATTCTTCT GCCCACCACC ATGCATTTAT 
CTTATTGGCC AAGGCTGGAA ACTCAAAAAG GATCGTGTCG CTCAACTGTA CAAAACACTC 
AAAGCTTCAG CTCAAAAGGA TGCTGCAATC GAGAATGATC CGATTCACGA GCAACAAGCC 
ACAGAACTCG TCGCCTACAT TGGAATCGGA TCAGACACTT CCGAGCGACA ACAACTTGAT 
TTTTCAACGG GAAAAGTTCG ACATCCTGGA GATCAGAGGC AGGATCCAAA TATTTATGAT 
TATTGTGCCG CGAAGACTCT GTATATTAGT GATTCGGACA AGAGAAAATA TTTCGATTTA 
AATGCTCAAT TCTTCTATGG ATGTGGAATG GAAATTGGAG GATTCGTATC ACAAAGAATC 
AAAGTAATCT CAAAACCATC GAAAAAGAAG CAAAGTATGA AAAATACGGA CTGCAAGTAT 
CTTTGTATTG CCAGTGGAAC CAAAGTTGCT CTATTCAATC GTCTCCGGTC ACAGACAGTC 
AGTACAAGAT ACCTTCATGT TGAGGGAAAT GCATTCCATG CTTCCTCCAC GAAATGGGGA 
GCCTTTACTA TTCATCTTTT TGACGACGAA CGTGGTTTGC AAGAGACTGA TAATTTTGCA 
GTTCGTGATG GATTTGTTTA CTATGGAAGT GTCGTTAAGC TTGTTGATAG TGTTACCGGA 
ATCGCACTGC CAAGATTGAG AATCAGAAAG GTCGATAAGC AGCAAGTAAT TCTTGATGCA 
TCGTGTAGTG AAGAACCAGT CTCTCAACTT CACAAATGTG CATTCCAAAT GATTGACAAT 
GAGCTCGTCT ATCTTTGTCT CAGTCATGAC AAGATTATTC AACATCAAGC CACAGCTATC 
AATGAGCATC GTCATCAGAT CAACGATGGA GCTGCGTGGA CAATCATCTC GACTGATAAG 
GCTGAATATC GATTCTTCGA AGCGATGGGA CAGGTTGCGA ACCCGATTTC TCCATGTCCA 
GTTGTCGGTT CACTTGAAGT TGATGGACAT GGTGAAGCGT CGCGTGTCGA GTTGCATGGA 
AGAGATTTTA AGCCAAATCT GAAAGTTTGG TTTGGAGCAA CCCCGGTTGA AACGACGTTC 
AGAAGTGAAG AGAGTCTTCA TTGTTCGATA CCACCTGTGA GTCAGGTTCG AAATGAGCAA 
ACTCACTGGA TGTTTACGAA TAGGACAACC GGCGATGTTG AGGTTCCAAT TTCATTGGTA 
CGAGATGATG GTGTCGTCTA CTCGTCGGGT CTCACATTTT CCTACAAATC ATTGGAACGA 
CATGGACCGT GCAGAATTGT GTCCAATTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293740.1
CDS	1..2373
Translation	MFSWRRGDCESQKEENRSEERKGEETIRFPTRSPFHCVLFLLTDGFVLHKPTASPTVGFSPTIFSFYYSRWESSHRISHDESGFCTAKTPLQDSTFTRHPSTSVPSSPSTPRHSGMDYHQSSSVASSESTASTVAAAAAAAAAASLNQHHHPHLYCDDGLLSRSLTDMVSSGGYDSSSSSLSAAASMCYPTPDAYYYHAPPPPPPPQAQQGFSSTDAWLQMQMQPTYHNFGSTVVSTNSPLPSHLLSYGGQPAFADPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001306811.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306811.1

ORF Insert Sequence:

ATGTTTTCGT GGAGGCGTGG CGATTGTGAG TCGCAGAAGG AAGAAAACCG AAGTGAGGAA 
AGGAAAGGAG AGGAGACTAT TCGTTTTCCC ACCCGCTCTC CTTTTCATTG TGTGCTCTTC 
TTGCTGACAG ACGGCTTCGT TCTTCACAAA CCCACAGCCA GCCCCACCGT CGGTTTTTCC 
CCCACAATAT TCAGCTTCTA CTATTCTCGG TGGGAATCTT CGCATCGGAT ATCCCACGAC 
GAGAGCGGCT TCTGCACCGC GAAGACCCCC CTTCAAGACT CGACTTTCAC GCGCCACCCA 
TCCACATCGG TCCCATCGTC GCCCTCAACG CCAAGACACT CCGGGATGGA TTATCATCAG 
TCGTCTTCGG TAGCTTCTTC CGAGTCGACA GCGTCTACAG TAGCCGCGGC GGCGGCTGCT 
GCAGCTGCTG CATCACTCAA TCAGCATCAT CATCCACATC TATATTGTGA TGATGGACTT 
CTATCGAGAA GTTTAACTGA TATGGTATCA TCTGGTGGAT ATGATAGCTC ATCATCTTCT 
CTAAGTGCAG CTGCATCAAT GTGTTATCCG ACTCCTGATG CATATTACTA TCATGCTCCA 
CCACCACCAC CTCCTCCACA AGCACAACAG GGATTCAGTT CAACAGACGC GTGGCTTCAA 
ATGCAAATGC AACCCACGTA TCATAACTTT GGCAGTACTG TCGTCTCTAC CAATTCCCCA 
CTTCCAAGCC ACCTTCTCAG TTATGGAGGA CAACCAGCAT TTGCTGATCC ATTCTACACA 
ATCGGGCAGT CAACTCCAAA CACATCATCA TTCCTGGACA CTTCAAATTC AAGTTTTGGA 
GCTCCGTCAA CAGTGGTTGC CAATCCGATG ACCAACTATC AATTGGCATT TCAAGCGAAA 
CTCGGAAGTC TTCACAGTTT GATTGGAGAT TCTGTTCAAT CGTTAACAAG CGATCGAATG 
ATTGATTTCT TGTCAAACAA GGAGAAGTAC GAATGCGTCA TATCGATTTT CCATGCCAAA 
GTTGCCCAAA AGTCATATGG AAATGAGAAA AGATTCTTCT GCCCACCACC ATGCATTTAT 
CTTATTGGCC AAGGCTGGAA ACTCAAAAAG GATCGTGTCG CTCAACTGTA CAAAACACTC 
AAAGCTTCAG CTCAAAAGGA TGCTGCAATC GAGAATGATC CGATTCACGA GCAACAAGCC 
ACAGAACTCG TCGCCTACAT TGGAATCGGA TCAGACACTT CCGAGCGACA ACAACTTGAT 
TTTTCAACGG GAAAAGTTCG ACATCCTGGA GATCAGAGGC AGGATCCAAA TATTTATGAT 
TATTGTGCCG CGAAGACTCT GTATATTAGT GATTCGGACA AGAGAAAATA TTTCGATTTA 
AATGCTCAAT TCTTCTATGG ATGTGGAATG GAAATTGGAG GATTCGTATC ACAAAGAATC 
AAAGTAATCT CAAAACCATC GAAAAAGAAG CAAAGTATGA AAAATACGGA CTGCAAGTAT 
CTTTGTATTG CCAGTGGAAC CAAAGTTGCT CTATTCAATC GTCTCCGGTC ACAGACAGTC 
AGTACAAGAT ACCTTCATGT TGAGGGAAAT GCATTCCATG CTTCCTCCAC GAAATGGGGA 
GCCTTTACTA TTCATCTTTT TGACGACGAA CGTGGTTTGC AAGAGACTGA TAATTTTGCA 
GTTCGTGATG GATTTGTTTA CTATGGAAGT GTCGTTAAGC TTGTTGATAG TGTTACCGGA 
ATCGCACTGC CAAGATTGAG AATCAGAAAG GTCGATAAGC AGCAAGTAAT TCTTGATGCA 
TCGTGTAGTG AAGAACCAGT CTCTCAACTT CACAAATGTG CATTCCAAAT GATTGACAAT 
GAGCTCGTCT ATCTTTGTCT CAGTCATGAC AAGATTATTC AACATCAAGC CACAGCTATC 
AATGAGCATC GTCATCAGAT CAACGATGGA GCTGCGTGGA CAATCATCTC GACTGATAAG 
GCTGAATATC GATTCTTCGA AGCGATGGGA CAGGTTGCGA ACCCGATTTC TCCATGTCCA 
GTTGTCGGTT CACTTGAAGT TGATGGACAT GGTGAAGCGT CGCGTGTCGA GTTGCATGGA 
AGAGATTTTA AGCCAAATCT GAAAGTTTGG TTTGGAGCAA CCCCGGTTGA AACGACGTTC 
AGAAGTGAAG AGAGTCTTCA TTGTTCGATA CCACCTGTGA GTCAGGTTCG AAATGAGCAA 
ACTCACTGGA TGTTTACGAA TAGGACAACC GGCGATGTTG AGGTTCCAAT TTCATTGGTA 
CGAGATGATG GTGTCGTCTA CTCGTCGGGT CTCACATTTT CCTACAAATC ATTGGAACGA 
CATGGACCGT GCAGAATTGT GTCCAATTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_001306811.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293740.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

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CloneID	OCa342673
Clone ID Related Accession (Same CDS sequence)	NM_001306811.1 , NM_001306811.2
Accession Version	NM_001306811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001306811.1. COMPLETENESS: incomplete on the 5' end.

ATGTTTTCGT GGAGGCGTGG CGATTGTGAG TCGCAGAAGG AAGAAAACCG AAGTGAGGAA 
AGGAAAGGAG AGGAGACTAT TCGTTTTCCC ACCCGCTCTC CTTTTCATTG TGTGCTCTTC 
TTGCTGACAG ACGGCTTCGT TCTTCACAAA CCCACAGCCA GCCCCACCGT CGGTTTTTCC 
CCCACAATAT TCAGCTTCTA CTATTCTCGG TGGGAATCTT CGCATCGGAT ATCCCACGAC 
GAGAGCGGCT TCTGCACCGC GAAGACCCCC CTTCAAGACT CGACTTTCAC GCGCCACCCA 
TCCACATCGG TCCCATCGTC GCCCTCAACG CCAAGACACT CCGGGATGGA TTATCATCAG 
TCGTCTTCGG TAGCTTCTTC CGAGTCGACA GCGTCTACAG TAGCCGCGGC GGCGGCTGCT 
GCAGCTGCTG CATCACTCAA TCAGCATCAT CATCCACATC TATATTGTGA TGATGGACTT 
CTATCGAGAA GTTTAACTGA TATGGTATCA TCTGGTGGAT ATGATAGCTC ATCATCTTCT 
CTAAGTGCAG CTGCATCAAT GTGTTATCCG ACTCCTGATG CATATTACTA TCATGCTCCA 
CCACCACCAC CTCCTCCACA AGCACAACAG GGATTCAGTT CAACAGACGC GTGGCTTCAA 
ATGCAAATGC AACCCACGTA TCATAACTTT GGCAGTACTG TCGTCTCTAC CAATTCCCCA 
CTTCCAAGCC ACCTTCTCAG TTATGGAGGA CAACCAGCAT TTGCTGATCC ATTCTACACA 
ATCGGGCAGT CAACTCCAAA CACATCATCA TTCCTGGACA CTTCAAATTC AAGTTTTGGA 
GCTCCGTCAA CAGTGGTTGC CAATCCGATG ACCAACTATC AATTGGCATT TCAAGCGAAA 
CTCGGAAGTC TTCACAGTTT GATTGGAGAT TCTGTTCAAT CGTTAACAAG CGATCGAATG 
ATTGATTTCT TGTCAAACAA GGAGAAGTAC GAATGCGTCA TATCGATTTT CCATGCCAAA 
GTTGCCCAAA AGTCATATGG AAATGAGAAA AGATTCTTCT GCCCACCACC ATGCATTTAT 
CTTATTGGCC AAGGCTGGAA ACTCAAAAAG GATCGTGTCG CTCAACTGTA CAAAACACTC 
AAAGCTTCAG CTCAAAAGGA TGCTGCAATC GAGAATGATC CGATTCACGA GCAACAAGCC 
ACAGAACTCG TCGCCTACAT TGGAATCGGA TCAGACACTT CCGAGCGACA ACAACTTGAT 
TTTTCAACGG GAAAAGTTCG ACATCCTGGA GATCAGAGGC AGGATCCAAA TATTTATGAT 
TATTGTGCCG CGAAGACTCT GTATATTAGT GATTCGGACA AGAGAAAATA TTTCGATTTA 
AATGCTCAAT TCTTCTATGG ATGTGGAATG GAAATTGGAG GATTCGTATC ACAAAGAATC 
AAAGTAATCT CAAAACCATC GAAAAAGAAG CAAAGTATGA AAAATACGGA CTGCAAGTAT 
CTTTGTATTG CCAGTGGAAC CAAAGTTGCT CTATTCAATC GTCTCCGGTC ACAGACAGTC 
AGTACAAGAT ACCTTCATGT TGAGGGAAAT GCATTCCATG CTTCCTCCAC GAAATGGGGA 
GCCTTTACTA TTCATCTTTT TGACGACGAA CGTGGTTTGC AAGAGACTGA TAATTTTGCA 
GTTCGTGATG GATTTGTTTA CTATGGAAGT GTCGTTAAGC TTGTTGATAG TGTTACCGGA 
ATCGCACTGC CAAGATTGAG AATCAGAAAG GTCGATAAGC AGCAAGTAAT TCTTGATGCA 
TCGTGTAGTG AAGAACCAGT CTCTCAACTT CACAAATGTG CATTCCAAAT GATTGACAAT 
GAGCTCGTCT ATCTTTGTCT CAGTCATGAC AAGATTATTC AACATCAAGC CACAGCTATC 
AATGAGCATC GTCATCAGAT CAACGATGGA GCTGCGTGGA CAATCATCTC GACTGATAAG 
GCTGAATATC GATTCTTCGA AGCGATGGGA CAGGTTGCGA ACCCGATTTC TCCATGTCCA 
GTTGTCGGTT CACTTGAAGT TGATGGACAT GGTGAAGCGT CGCGTGTCGA GTTGCATGGA 
AGAGATTTTA AGCCAAATCT GAAAGTTTGG TTTGGAGCAA CCCCGGTTGA AACGACGTTC 
AGAAGTGAAG AGAGTCTTCA TTGTTCGATA CCACCTGTGA GTCAGGTTCG AAATGAGCAA 
ACTCACTGGA TGTTTACGAA TAGGACAACC GGCGATGTTG AGGTTCCAAT TTCATTGGTA 
CGAGATGATG GTGTCGTCTA CTCGTCGGGT CTCACATTTT CCTACAAATC ATTGGAACGA 
CATGGACCGT GCAGAATTGT GTCCAATTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293740.1
CDS	1..2373
Translation	MFSWRRGDCESQKEENRSEERKGEETIRFPTRSPFHCVLFLLTDGFVLHKPTASPTVGFSPTIFSFYYSRWESSHRISHDESGFCTAKTPLQDSTFTRHPSTSVPSSPSTPRHSGMDYHQSSSVASSESTASTVAAAAAAAAAASLNQHHHPHLYCDDGLLSRSLTDMVSSGGYDSSSSSLSAAASMCYPTPDAYYYHAPPPPPPPQAQQGFSSTDAWLQMQMQPTYHNFGSTVVSTNSPLPSHLLSYGGQPAFADPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001306811.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306811.2

ORF Insert Sequence:

ATGTTTTCGT GGAGGCGTGG CGATTGTGAG TCGCAGAAGG AAGAAAACCG AAGTGAGGAA 
AGGAAAGGAG AGGAGACTAT TCGTTTTCCC ACCCGCTCTC CTTTTCATTG TGTGCTCTTC 
TTGCTGACAG ACGGCTTCGT TCTTCACAAA CCCACAGCCA GCCCCACCGT CGGTTTTTCC 
CCCACAATAT TCAGCTTCTA CTATTCTCGG TGGGAATCTT CGCATCGGAT ATCCCACGAC 
GAGAGCGGCT TCTGCACCGC GAAGACCCCC CTTCAAGACT CGACTTTCAC GCGCCACCCA 
TCCACATCGG TCCCATCGTC GCCCTCAACG CCAAGACACT CCGGGATGGA TTATCATCAG 
TCGTCTTCGG TAGCTTCTTC CGAGTCGACA GCGTCTACAG TAGCCGCGGC GGCGGCTGCT 
GCAGCTGCTG CATCACTCAA TCAGCATCAT CATCCACATC TATATTGTGA TGATGGACTT 
CTATCGAGAA GTTTAACTGA TATGGTATCA TCTGGTGGAT ATGATAGCTC ATCATCTTCT 
CTAAGTGCAG CTGCATCAAT GTGTTATCCG ACTCCTGATG CATATTACTA TCATGCTCCA 
CCACCACCAC CTCCTCCACA AGCACAACAG GGATTCAGTT CAACAGACGC GTGGCTTCAA 
ATGCAAATGC AACCCACGTA TCATAACTTT GGCAGTACTG TCGTCTCTAC CAATTCCCCA 
CTTCCAAGCC ACCTTCTCAG TTATGGAGGA CAACCAGCAT TTGCTGATCC ATTCTACACA 
ATCGGGCAGT CAACTCCAAA CACATCATCA TTCCTGGACA CTTCAAATTC AAGTTTTGGA 
GCTCCGTCAA CAGTGGTTGC CAATCCGATG ACCAACTATC AATTGGCATT TCAAGCGAAA 
CTCGGAAGTC TTCACAGTTT GATTGGAGAT TCTGTTCAAT CGTTAACAAG CGATCGAATG 
ATTGATTTCT TGTCAAACAA GGAGAAGTAC GAATGCGTCA TATCGATTTT CCATGCCAAA 
GTTGCCCAAA AGTCATATGG AAATGAGAAA AGATTCTTCT GCCCACCACC ATGCATTTAT 
CTTATTGGCC AAGGCTGGAA ACTCAAAAAG GATCGTGTCG CTCAACTGTA CAAAACACTC 
AAAGCTTCAG CTCAAAAGGA TGCTGCAATC GAGAATGATC CGATTCACGA GCAACAAGCC 
ACAGAACTCG TCGCCTACAT TGGAATCGGA TCAGACACTT CCGAGCGACA ACAACTTGAT 
TTTTCAACGG GAAAAGTTCG ACATCCTGGA GATCAGAGGC AGGATCCAAA TATTTATGAT 
TATTGTGCCG CGAAGACTCT GTATATTAGT GATTCGGACA AGAGAAAATA TTTCGATTTA 
AATGCTCAAT TCTTCTATGG ATGTGGAATG GAAATTGGAG GATTCGTATC ACAAAGAATC 
AAAGTAATCT CAAAACCATC GAAAAAGAAG CAAAGTATGA AAAATACGGA CTGCAAGTAT 
CTTTGTATTG CCAGTGGAAC CAAAGTTGCT CTATTCAATC GTCTCCGGTC ACAGACAGTC 
AGTACAAGAT ACCTTCATGT TGAGGGAAAT GCATTCCATG CTTCCTCCAC GAAATGGGGA 
GCCTTTACTA TTCATCTTTT TGACGACGAA CGTGGTTTGC AAGAGACTGA TAATTTTGCA 
GTTCGTGATG GATTTGTTTA CTATGGAAGT GTCGTTAAGC TTGTTGATAG TGTTACCGGA 
ATCGCACTGC CAAGATTGAG AATCAGAAAG GTCGATAAGC AGCAAGTAAT TCTTGATGCA 
TCGTGTAGTG AAGAACCAGT CTCTCAACTT CACAAATGTG CATTCCAAAT GATTGACAAT 
GAGCTCGTCT ATCTTTGTCT CAGTCATGAC AAGATTATTC AACATCAAGC CACAGCTATC 
AATGAGCATC GTCATCAGAT CAACGATGGA GCTGCGTGGA CAATCATCTC GACTGATAAG 
GCTGAATATC GATTCTTCGA AGCGATGGGA CAGGTTGCGA ACCCGATTTC TCCATGTCCA 
GTTGTCGGTT CACTTGAAGT TGATGGACAT GGTGAAGCGT CGCGTGTCGA GTTGCATGGA 
AGAGATTTTA AGCCAAATCT GAAAGTTTGG TTTGGAGCAA CCCCGGTTGA AACGACGTTC 
AGAAGTGAAG AGAGTCTTCA TTGTTCGATA CCACCTGTGA GTCAGGTTCG AAATGAGCAA 
ACTCACTGGA TGTTTACGAA TAGGACAACC GGCGATGTTG AGGTTCCAAT TTCATTGGTA 
CGAGATGATG GTGTCGTCTA CTCGTCGGGT CTCACATTTT CCTACAAATC ATTGGAACGA 
CATGGACCGT GCAGAATTGT GTCCAATTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_171350.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741410.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

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CloneID	OCa344078
Clone ID Related Accession (Same CDS sequence)	NM_171350.4 , NM_171350.5
Accession Version	NM_171350.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_171350.3. COMPLETENESS: incomplete on both ends.

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGTTC CAGAAGCAAT GTTCCAGTCG GTATCGCCGC TGGCCGGTGT CGCTGCAGCG 
CCGAGCCAAA TTGCCGCACT TCAACAGATA CAAGCGTTGA TGACATTTCA AATGCAGCAG 
AATAATTTAT TTCCCAAAAT TGACACTATA TCGAAATCGC CAACTCCAGA ACTCGCATCT 
CCATCGGCAA AACGAATGAG ACTTTCTCCC AGTACGAGTA GCCACAGTGA TGTCGCATCA 
ACTTCGAAAG GAACAAATGG GCAAGATCAG TCGAAAAATT CGCCGACAAA TTCTGATCCA 
TTCTACACAA TCGGGCAGTC AACTCCAAAC ACATCATCAT TCCTGGACAC TTCAAATTCA 
AGTTTTGGAG CTCCGTCAAC AGTGGTTGCC AATCCGATGA CCAACTATCA ATTGGCATTT 
CAAGCGAAAC TCGGAAGTCT TCACAGTTTG ATTGGAGATT CTGTTCAATC GTTAACAAGC 
GATCGAATGA TTGATTTCTT GTCAAACAAG GAGAAGTACG AATGCGTCAT ATCGATTTTC 
CATGCCAAAG TTGCCCAAAA GTCATATGGA AATGAGAAAA GATTCTTCTG CCCACCACCA 
TGCATTTATC TTATTGGCCA AGGCTGGAAA CTCAAAAAGG ATCGTGTCGC TCAACTGTAC 
AAAACACTCA AAGCTTCAGC TCAAAAGGAT GCTGCAATCG AGAATGATCC GATTCACGAG 
CAACAAGCCA CAGAACTCGT CGCCTACATT GGAATCGGAT CAGACACTTC CGAGCGACAA 
CAACTTGATT TTTCAACGGG AAAAGTTCGA CATCCTGGAG ATCAGAGGCA GGATCCAAAT 
ATTTATGATT ATTGTGCCGC GAAGACTCTG TATATTAGTG ATTCGGACAA GAGAAAATAT 
TTCGATTTAA ATGCTCAATT CTTCTATGGA TGTGGAATGG AAATTGGAGG ATTCGTATCA 
CAAAGAATCA AAGTAATCTC AAAACCATCG AAAAAGAAGC AAAGTATGAA AAATACGGAC 
TGCAAGTATC TTTGTATTGC CAGTGGAACC AAAGTTGCTC TATTCAATCG TCTCCGGTCA 
CAGACAGTCA GTACAAGATA CCTTCATGTT GAGGGAAATG CATTCCATGC TTCCTCCACG 
AAATGGGGAG CCTTTACTAT TCATCTTTTT GACGACGAAC GTGGTTTGCA AGAGACTGAT 
AATTTTGCAG TTCGTGATGG ATTTGTTTAC TATGGAAGTG TCGTTAAGCT TGTTGATAGT 
GTTACCGGAA TCGCACTGCC AAGATTGAGA ATCAGAAAGG TCGATAAGCA GCAAGTAATT 
CTTGATGCAT CGTGTAGTGA AGAACCAGTC TCTCAACTTC ACAAATGTGC ATTCCAAATG 
ATTGACAATG AGCTCGTCTA TCTTTGTCTC AGTCATGACA AGATTATTCA ACATCAAGCC 
ACAGCTATCA ATGAGCATCG TCATCAGATC AACGATGGAG CTGCGTGGAC AATCATCTCG 
ACTGATAAGG CTGAATATCG ATTCTTCGAA GCGATGGGAC AGGTTGCGAA CCCGATTTCT 
CCATGTCCAG TTGTCGGTTC ACTTGAAGTT GATGGACATG GTGAAGCGTC GCGTGTCGAG 
TTGCATGGAA GAGATTTTAA GCCAAATCTG AAAGTTTGGT TTGGAGCAAC CCCGGTTGAA 
ACGACGTTCA GAAGTGAAGA GAGTCTTCAT TGTTCGATAC CACCTGTGAG TCAGGTTCGA 
AATGAGCAAA CTCACTGGAT GTTTACGAAT AGGACAACCG GCGATGTTGA GGTTCCAATT 
TCATTGGTAC GAGATGATGG TGTCGTCTAC TCGTCGGGTC TCACATTTTC CTACAAATCA 
TTGGAACGAC ATGGACCGTG CAGAATTGTG TCCAATTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741410.1
CDS	1..2022
Translation	MPLAYSTHDNFYESPKTPQPTWDHVHAQFPLGEPARNLDKFIVPEAMFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_171350.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171350.4

ORF Insert Sequence:

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGTTC CAGAAGCAAT GTTCCAGTCG GTATCGCCGC TGGCCGGTGT CGCTGCAGCG 
CCGAGCCAAA TTGCCGCACT TCAACAGATA CAAGCGTTGA TGACATTTCA AATGCAGCAG 
AATAATTTAT TTCCCAAAAT TGACACTATA TCGAAATCGC CAACTCCAGA ACTCGCATCT 
CCATCGGCAA AACGAATGAG ACTTTCTCCC AGTACGAGTA GCCACAGTGA TGTCGCATCA 
ACTTCGAAAG GAACAAATGG GCAAGATCAG TCGAAAAATT CGCCGACAAA TTCTGATCCA 
TTCTACACAA TCGGGCAGTC AACTCCAAAC ACATCATCAT TCCTGGACAC TTCAAATTCA 
AGTTTTGGAG CTCCGTCAAC AGTGGTTGCC AATCCGATGA CCAACTATCA ATTGGCATTT 
CAAGCGAAAC TCGGAAGTCT TCACAGTTTG ATTGGAGATT CTGTTCAATC GTTAACAAGC 
GATCGAATGA TTGATTTCTT GTCAAACAAG GAGAAGTACG AATGCGTCAT ATCGATTTTC 
CATGCCAAAG TTGCCCAAAA GTCATATGGA AATGAGAAAA GATTCTTCTG CCCACCACCA 
TGCATTTATC TTATTGGCCA AGGCTGGAAA CTCAAAAAGG ATCGTGTCGC TCAACTGTAC 
AAAACACTCA AAGCTTCAGC TCAAAAGGAT GCTGCAATCG AGAATGATCC GATTCACGAG 
CAACAAGCCA CAGAACTCGT CGCCTACATT GGAATCGGAT CAGACACTTC CGAGCGACAA 
CAACTTGATT TTTCAACGGG AAAAGTTCGA CATCCTGGAG ATCAGAGGCA GGATCCAAAT 
ATTTATGATT ATTGTGCCGC GAAGACTCTG TATATTAGTG ATTCGGACAA GAGAAAATAT 
TTCGATTTAA ATGCTCAATT CTTCTATGGA TGTGGAATGG AAATTGGAGG ATTCGTATCA 
CAAAGAATCA AAGTAATCTC AAAACCATCG AAAAAGAAGC AAAGTATGAA AAATACGGAC 
TGCAAGTATC TTTGTATTGC CAGTGGAACC AAAGTTGCTC TATTCAATCG TCTCCGGTCA 
CAGACAGTCA GTACAAGATA CCTTCATGTT GAGGGAAATG CATTCCATGC TTCCTCCACG 
AAATGGGGAG CCTTTACTAT TCATCTTTTT GACGACGAAC GTGGTTTGCA AGAGACTGAT 
AATTTTGCAG TTCGTGATGG ATTTGTTTAC TATGGAAGTG TCGTTAAGCT TGTTGATAGT 
GTTACCGGAA TCGCACTGCC AAGATTGAGA ATCAGAAAGG TCGATAAGCA GCAAGTAATT 
CTTGATGCAT CGTGTAGTGA AGAACCAGTC TCTCAACTTC ACAAATGTGC ATTCCAAATG 
ATTGACAATG AGCTCGTCTA TCTTTGTCTC AGTCATGACA AGATTATTCA ACATCAAGCC 
ACAGCTATCA ATGAGCATCG TCATCAGATC AACGATGGAG CTGCGTGGAC AATCATCTCG 
ACTGATAAGG CTGAATATCG ATTCTTCGAA GCGATGGGAC AGGTTGCGAA CCCGATTTCT 
CCATGTCCAG TTGTCGGTTC ACTTGAAGTT GATGGACATG GTGAAGCGTC GCGTGTCGAG 
TTGCATGGAA GAGATTTTAA GCCAAATCTG AAAGTTTGGT TTGGAGCAAC CCCGGTTGAA 
ACGACGTTCA GAAGTGAAGA GAGTCTTCAT TGTTCGATAC CACCTGTGAG TCAGGTTCGA 
AATGAGCAAA CTCACTGGAT GTTTACGAAT AGGACAACCG GCGATGTTGA GGTTCCAATT 
TCATTGGTAC GAGATGATGG TGTCGTCTAC TCGTCGGGTC TCACATTTTC CTACAAATCA 
TTGGAACGAC ATGGACCGTG CAGAATTGTG TCCAATTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_171350.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741410.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

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CloneID	OCa344078
Clone ID Related Accession (Same CDS sequence)	NM_171350.4 , NM_171350.5
Accession Version	NM_171350.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_171350.4.

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGTTC CAGAAGCAAT GTTCCAGTCG GTATCGCCGC TGGCCGGTGT CGCTGCAGCG 
CCGAGCCAAA TTGCCGCACT TCAACAGATA CAAGCGTTGA TGACATTTCA AATGCAGCAG 
AATAATTTAT TTCCCAAAAT TGACACTATA TCGAAATCGC CAACTCCAGA ACTCGCATCT 
CCATCGGCAA AACGAATGAG ACTTTCTCCC AGTACGAGTA GCCACAGTGA TGTCGCATCA 
ACTTCGAAAG GAACAAATGG GCAAGATCAG TCGAAAAATT CGCCGACAAA TTCTGATCCA 
TTCTACACAA TCGGGCAGTC AACTCCAAAC ACATCATCAT TCCTGGACAC TTCAAATTCA 
AGTTTTGGAG CTCCGTCAAC AGTGGTTGCC AATCCGATGA CCAACTATCA ATTGGCATTT 
CAAGCGAAAC TCGGAAGTCT TCACAGTTTG ATTGGAGATT CTGTTCAATC GTTAACAAGC 
GATCGAATGA TTGATTTCTT GTCAAACAAG GAGAAGTACG AATGCGTCAT ATCGATTTTC 
CATGCCAAAG TTGCCCAAAA GTCATATGGA AATGAGAAAA GATTCTTCTG CCCACCACCA 
TGCATTTATC TTATTGGCCA AGGCTGGAAA CTCAAAAAGG ATCGTGTCGC TCAACTGTAC 
AAAACACTCA AAGCTTCAGC TCAAAAGGAT GCTGCAATCG AGAATGATCC GATTCACGAG 
CAACAAGCCA CAGAACTCGT CGCCTACATT GGAATCGGAT CAGACACTTC CGAGCGACAA 
CAACTTGATT TTTCAACGGG AAAAGTTCGA CATCCTGGAG ATCAGAGGCA GGATCCAAAT 
ATTTATGATT ATTGTGCCGC GAAGACTCTG TATATTAGTG ATTCGGACAA GAGAAAATAT 
TTCGATTTAA ATGCTCAATT CTTCTATGGA TGTGGAATGG AAATTGGAGG ATTCGTATCA 
CAAAGAATCA AAGTAATCTC AAAACCATCG AAAAAGAAGC AAAGTATGAA AAATACGGAC 
TGCAAGTATC TTTGTATTGC CAGTGGAACC AAAGTTGCTC TATTCAATCG TCTCCGGTCA 
CAGACAGTCA GTACAAGATA CCTTCATGTT GAGGGAAATG CATTCCATGC TTCCTCCACG 
AAATGGGGAG CCTTTACTAT TCATCTTTTT GACGACGAAC GTGGTTTGCA AGAGACTGAT 
AATTTTGCAG TTCGTGATGG ATTTGTTTAC TATGGAAGTG TCGTTAAGCT TGTTGATAGT 
GTTACCGGAA TCGCACTGCC AAGATTGAGA ATCAGAAAGG TCGATAAGCA GCAAGTAATT 
CTTGATGCAT CGTGTAGTGA AGAACCAGTC TCTCAACTTC ACAAATGTGC ATTCCAAATG 
ATTGACAATG AGCTCGTCTA TCTTTGTCTC AGTCATGACA AGATTATTCA ACATCAAGCC 
ACAGCTATCA ATGAGCATCG TCATCAGATC AACGATGGAG CTGCGTGGAC AATCATCTCG 
ACTGATAAGG CTGAATATCG ATTCTTCGAA GCGATGGGAC AGGTTGCGAA CCCGATTTCT 
CCATGTCCAG TTGTCGGTTC ACTTGAAGTT GATGGACATG GTGAAGCGTC GCGTGTCGAG 
TTGCATGGAA GAGATTTTAA GCCAAATCTG AAAGTTTGGT TTGGAGCAAC CCCGGTTGAA 
ACGACGTTCA GAAGTGAAGA GAGTCTTCAT TGTTCGATAC CACCTGTGAG TCAGGTTCGA 
AATGAGCAAA CTCACTGGAT GTTTACGAAT AGGACAACCG GCGATGTTGA GGTTCCAATT 
TCATTGGTAC GAGATGATGG TGTCGTCTAC TCGTCGGGTC TCACATTTTC CTACAAATCA 
TTGGAACGAC ATGGACCGTG CAGAATTGTG TCCAATTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741410.1
CDS	4..2025
Translation	MPLAYSTHDNFYESPKTPQPTWDHVHAQFPLGEPARNLDKFIVPEAMFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_171350.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171350.5

ORF Insert Sequence:

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGTTC CAGAAGCAAT GTTCCAGTCG GTATCGCCGC TGGCCGGTGT CGCTGCAGCG 
CCGAGCCAAA TTGCCGCACT TCAACAGATA CAAGCGTTGA TGACATTTCA AATGCAGCAG 
AATAATTTAT TTCCCAAAAT TGACACTATA TCGAAATCGC CAACTCCAGA ACTCGCATCT 
CCATCGGCAA AACGAATGAG ACTTTCTCCC AGTACGAGTA GCCACAGTGA TGTCGCATCA 
ACTTCGAAAG GAACAAATGG GCAAGATCAG TCGAAAAATT CGCCGACAAA TTCTGATCCA 
TTCTACACAA TCGGGCAGTC AACTCCAAAC ACATCATCAT TCCTGGACAC TTCAAATTCA 
AGTTTTGGAG CTCCGTCAAC AGTGGTTGCC AATCCGATGA CCAACTATCA ATTGGCATTT 
CAAGCGAAAC TCGGAAGTCT TCACAGTTTG ATTGGAGATT CTGTTCAATC GTTAACAAGC 
GATCGAATGA TTGATTTCTT GTCAAACAAG GAGAAGTACG AATGCGTCAT ATCGATTTTC 
CATGCCAAAG TTGCCCAAAA GTCATATGGA AATGAGAAAA GATTCTTCTG CCCACCACCA 
TGCATTTATC TTATTGGCCA AGGCTGGAAA CTCAAAAAGG ATCGTGTCGC TCAACTGTAC 
AAAACACTCA AAGCTTCAGC TCAAAAGGAT GCTGCAATCG AGAATGATCC GATTCACGAG 
CAACAAGCCA CAGAACTCGT CGCCTACATT GGAATCGGAT CAGACACTTC CGAGCGACAA 
CAACTTGATT TTTCAACGGG AAAAGTTCGA CATCCTGGAG ATCAGAGGCA GGATCCAAAT 
ATTTATGATT ATTGTGCCGC GAAGACTCTG TATATTAGTG ATTCGGACAA GAGAAAATAT 
TTCGATTTAA ATGCTCAATT CTTCTATGGA TGTGGAATGG AAATTGGAGG ATTCGTATCA 
CAAAGAATCA AAGTAATCTC AAAACCATCG AAAAAGAAGC AAAGTATGAA AAATACGGAC 
TGCAAGTATC TTTGTATTGC CAGTGGAACC AAAGTTGCTC TATTCAATCG TCTCCGGTCA 
CAGACAGTCA GTACAAGATA CCTTCATGTT GAGGGAAATG CATTCCATGC TTCCTCCACG 
AAATGGGGAG CCTTTACTAT TCATCTTTTT GACGACGAAC GTGGTTTGCA AGAGACTGAT 
AATTTTGCAG TTCGTGATGG ATTTGTTTAC TATGGAAGTG TCGTTAAGCT TGTTGATAGT 
GTTACCGGAA TCGCACTGCC AAGATTGAGA ATCAGAAAGG TCGATAAGCA GCAAGTAATT 
CTTGATGCAT CGTGTAGTGA AGAACCAGTC TCTCAACTTC ACAAATGTGC ATTCCAAATG 
ATTGACAATG AGCTCGTCTA TCTTTGTCTC AGTCATGACA AGATTATTCA ACATCAAGCC 
ACAGCTATCA ATGAGCATCG TCATCAGATC AACGATGGAG CTGCGTGGAC AATCATCTCG 
ACTGATAAGG CTGAATATCG ATTCTTCGAA GCGATGGGAC AGGTTGCGAA CCCGATTTCT 
CCATGTCCAG TTGTCGGTTC ACTTGAAGTT GATGGACATG GTGAAGCGTC GCGTGTCGAG 
TTGCATGGAA GAGATTTTAA GCCAAATCTG AAAGTTTGGT TTGGAGCAAC CCCGGTTGAA 
ACGACGTTCA GAAGTGAAGA GAGTCTTCAT TGTTCGATAC CACCTGTGAG TCAGGTTCGA 
AATGAGCAAA CTCACTGGAT GTTTACGAAT AGGACAACCG GCGATGTTGA GGTTCCAATT 
TCATTGGTAC GAGATGATGG TGTCGTCTAC TCGTCGGGTC TCACATTTTC CTACAAATCA 
TTGGAACGAC ATGGACCGTG CAGAATTGTG TCCAATTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_001028107.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023278.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Price & Availability↑

CloneID	OCa344079
Clone ID Related Accession (Same CDS sequence)	NM_001028107.3 , NM_001028107.4
Accession Version	NM_001028107.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001028107.2. COMPLETENESS: incomplete on both ends.

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGAAG CAATGTTCCA GTCGGTATCG CCGCTGGCCG GTGTCGCTGC AGCGCCGAGC 
CAAATTGCCG CACTTCAACA GATACAAGCG TTGATGACAT TTCAAATGCA GCAGAATAAT 
TTATTTCCCA AAATTGACAC TATATCGAAA TCGCCAACTC CAGAACTCGC ATCTCCATCG 
GCAAAACGAA TGAGACTTTC TCCCAGTACG AGTAGCCACA GTGATGTCGC ATCAACTTCG 
AAAGGAACAA ATGGGCAAGA TCAGTCGAAA AATTCGCCGA CAAATTCTGA TCCATTCTAC 
ACAATCGGGC AGTCAACTCC AAACACATCA TCATTCCTGG ACACTTCAAA TTCAAGTTTT 
GGAGCTCCGT CAACAGTGGT TGCCAATCCG ATGACCAACT ATCAATTGGC ATTTCAAGCG 
AAACTCGGAA GTCTTCACAG TTTGATTGGA GATTCTGTTC AATCGTTAAC AAGCGATCGA 
ATGATTGATT TCTTGTCAAA CAAGGAGAAG TACGAATGCG TCATATCGAT TTTCCATGCC 
AAAGTTGCCC AAAAGTCATA TGGAAATGAG AAAAGATTCT TCTGCCCACC ACCATGCATT 
TATCTTATTG GCCAAGGCTG GAAACTCAAA AAGGATCGTG TCGCTCAACT GTACAAAACA 
CTCAAAGCTT CAGCTCAAAA GGATGCTGCA ATCGAGAATG ATCCGATTCA CGAGCAACAA 
GCCACAGAAC TCGTCGCCTA CATTGGAATC GGATCAGACA CTTCCGAGCG ACAACAACTT 
GATTTTTCAA CGGGAAAAGT TCGACATCCT GGAGATCAGA GGCAGGATCC AAATATTTAT 
GATTATTGTG CCGCGAAGAC TCTGTATATT AGTGATTCGG ACAAGAGAAA ATATTTCGAT 
TTAAATGCTC AATTCTTCTA TGGATGTGGA ATGGAAATTG GAGGATTCGT ATCACAAAGA 
ATCAAAGTAA TCTCAAAACC ATCGAAAAAG AAGCAAAGTA TGAAAAATAC GGACTGCAAG 
TATCTTTGTA TTGCCAGTGG AACCAAAGTT GCTCTATTCA ATCGTCTCCG GTCACAGACA 
GTCAGTACAA GATACCTTCA TGTTGAGGGA AATGCATTCC ATGCTTCCTC CACGAAATGG 
GGAGCCTTTA CTATTCATCT TTTTGACGAC GAACGTGGTT TGCAAGAGAC TGATAATTTT 
GCAGTTCGTG ATGGATTTGT TTACTATGGA AGTGTCGTTA AGCTTGTTGA TAGTGTTACC 
GGAATCGCAC TGCCAAGATT GAGAATCAGA AAGGTCGATA AGCAGCAAGT AATTCTTGAT 
GCATCGTGTA GTGAAGAACC AGTCTCTCAA CTTCACAAAT GTGCATTCCA AATGATTGAC 
AATGAGCTCG TCTATCTTTG TCTCAGTCAT GACAAGATTA TTCAACATCA AGCCACAGCT 
ATCAATGAGC ATCGTCATCA GATCAACGAT GGAGCTGCGT GGACAATCAT CTCGACTGAT 
AAGGCTGAAT ATCGATTCTT CGAAGCGATG GGACAGGTTG CGAACCCGAT TTCTCCATGT 
CCAGTTGTCG GTTCACTTGA AGTTGATGGA CATGGTGAAG CGTCGCGTGT CGAGTTGCAT 
GGAAGAGATT TTAAGCCAAA TCTGAAAGTT TGGTTTGGAG CAACCCCGGT TGAAACGACG 
TTCAGAAGTG AAGAGAGTCT TCATTGTTCG ATACCACCTG TGAGTCAGGT TCGAAATGAG 
CAAACTCACT GGATGTTTAC GAATAGGACA ACCGGCGATG TTGAGGTTCC AATTTCATTG 
GTACGAGATG ATGGTGTCGT CTACTCGTCG GGTCTCACAT TTTCCTACAA ATCATTGGAA 
CGACATGGAC CGTGCAGAAT TGTGTCCAAT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023278.1
CDS	1..2016
Translation	MPLAYSTHDNFYESPKTPQPTWDHVHAQFPLGEPARNLDKFIEAMFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001028107.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028107.3

ORF Insert Sequence:

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGAAG CAATGTTCCA GTCGGTATCG CCGCTGGCCG GTGTCGCTGC AGCGCCGAGC 
CAAATTGCCG CACTTCAACA GATACAAGCG TTGATGACAT TTCAAATGCA GCAGAATAAT 
TTATTTCCCA AAATTGACAC TATATCGAAA TCGCCAACTC CAGAACTCGC ATCTCCATCG 
GCAAAACGAA TGAGACTTTC TCCCAGTACG AGTAGCCACA GTGATGTCGC ATCAACTTCG 
AAAGGAACAA ATGGGCAAGA TCAGTCGAAA AATTCGCCGA CAAATTCTGA TCCATTCTAC 
ACAATCGGGC AGTCAACTCC AAACACATCA TCATTCCTGG ACACTTCAAA TTCAAGTTTT 
GGAGCTCCGT CAACAGTGGT TGCCAATCCG ATGACCAACT ATCAATTGGC ATTTCAAGCG 
AAACTCGGAA GTCTTCACAG TTTGATTGGA GATTCTGTTC AATCGTTAAC AAGCGATCGA 
ATGATTGATT TCTTGTCAAA CAAGGAGAAG TACGAATGCG TCATATCGAT TTTCCATGCC 
AAAGTTGCCC AAAAGTCATA TGGAAATGAG AAAAGATTCT TCTGCCCACC ACCATGCATT 
TATCTTATTG GCCAAGGCTG GAAACTCAAA AAGGATCGTG TCGCTCAACT GTACAAAACA 
CTCAAAGCTT CAGCTCAAAA GGATGCTGCA ATCGAGAATG ATCCGATTCA CGAGCAACAA 
GCCACAGAAC TCGTCGCCTA CATTGGAATC GGATCAGACA CTTCCGAGCG ACAACAACTT 
GATTTTTCAA CGGGAAAAGT TCGACATCCT GGAGATCAGA GGCAGGATCC AAATATTTAT 
GATTATTGTG CCGCGAAGAC TCTGTATATT AGTGATTCGG ACAAGAGAAA ATATTTCGAT 
TTAAATGCTC AATTCTTCTA TGGATGTGGA ATGGAAATTG GAGGATTCGT ATCACAAAGA 
ATCAAAGTAA TCTCAAAACC ATCGAAAAAG AAGCAAAGTA TGAAAAATAC GGACTGCAAG 
TATCTTTGTA TTGCCAGTGG AACCAAAGTT GCTCTATTCA ATCGTCTCCG GTCACAGACA 
GTCAGTACAA GATACCTTCA TGTTGAGGGA AATGCATTCC ATGCTTCCTC CACGAAATGG 
GGAGCCTTTA CTATTCATCT TTTTGACGAC GAACGTGGTT TGCAAGAGAC TGATAATTTT 
GCAGTTCGTG ATGGATTTGT TTACTATGGA AGTGTCGTTA AGCTTGTTGA TAGTGTTACC 
GGAATCGCAC TGCCAAGATT GAGAATCAGA AAGGTCGATA AGCAGCAAGT AATTCTTGAT 
GCATCGTGTA GTGAAGAACC AGTCTCTCAA CTTCACAAAT GTGCATTCCA AATGATTGAC 
AATGAGCTCG TCTATCTTTG TCTCAGTCAT GACAAGATTA TTCAACATCA AGCCACAGCT 
ATCAATGAGC ATCGTCATCA GATCAACGAT GGAGCTGCGT GGACAATCAT CTCGACTGAT 
AAGGCTGAAT ATCGATTCTT CGAAGCGATG GGACAGGTTG CGAACCCGAT TTCTCCATGT 
CCAGTTGTCG GTTCACTTGA AGTTGATGGA CATGGTGAAG CGTCGCGTGT CGAGTTGCAT 
GGAAGAGATT TTAAGCCAAA TCTGAAAGTT TGGTTTGGAG CAACCCCGGT TGAAACGACG 
TTCAGAAGTG AAGAGAGTCT TCATTGTTCG ATACCACCTG TGAGTCAGGT TCGAAATGAG 
CAAACTCACT GGATGTTTAC GAATAGGACA ACCGGCGATG TTGAGGTTCC AATTTCATTG 
GTACGAGATG ATGGTGTCGT CTACTCGTCG GGTCTCACAT TTTCCTACAA ATCATTGGAA 
CGACATGGAC CGTGCAGAAT TGTGTCCAAT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lag-1 ( NM_001028107.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001023278.1 Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

Price & Availability↑

CloneID	OCa344079
Clone ID Related Accession (Same CDS sequence)	NM_001028107.3 , NM_001028107.4
Accession Version	NM_001028107.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Lin-12 And Glp-1 phenotype
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001028107.3.

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGAAG CAATGTTCCA GTCGGTATCG CCGCTGGCCG GTGTCGCTGC AGCGCCGAGC 
CAAATTGCCG CACTTCAACA GATACAAGCG TTGATGACAT TTCAAATGCA GCAGAATAAT 
TTATTTCCCA AAATTGACAC TATATCGAAA TCGCCAACTC CAGAACTCGC ATCTCCATCG 
GCAAAACGAA TGAGACTTTC TCCCAGTACG AGTAGCCACA GTGATGTCGC ATCAACTTCG 
AAAGGAACAA ATGGGCAAGA TCAGTCGAAA AATTCGCCGA CAAATTCTGA TCCATTCTAC 
ACAATCGGGC AGTCAACTCC AAACACATCA TCATTCCTGG ACACTTCAAA TTCAAGTTTT 
GGAGCTCCGT CAACAGTGGT TGCCAATCCG ATGACCAACT ATCAATTGGC ATTTCAAGCG 
AAACTCGGAA GTCTTCACAG TTTGATTGGA GATTCTGTTC AATCGTTAAC AAGCGATCGA 
ATGATTGATT TCTTGTCAAA CAAGGAGAAG TACGAATGCG TCATATCGAT TTTCCATGCC 
AAAGTTGCCC AAAAGTCATA TGGAAATGAG AAAAGATTCT TCTGCCCACC ACCATGCATT 
TATCTTATTG GCCAAGGCTG GAAACTCAAA AAGGATCGTG TCGCTCAACT GTACAAAACA 
CTCAAAGCTT CAGCTCAAAA GGATGCTGCA ATCGAGAATG ATCCGATTCA CGAGCAACAA 
GCCACAGAAC TCGTCGCCTA CATTGGAATC GGATCAGACA CTTCCGAGCG ACAACAACTT 
GATTTTTCAA CGGGAAAAGT TCGACATCCT GGAGATCAGA GGCAGGATCC AAATATTTAT 
GATTATTGTG CCGCGAAGAC TCTGTATATT AGTGATTCGG ACAAGAGAAA ATATTTCGAT 
TTAAATGCTC AATTCTTCTA TGGATGTGGA ATGGAAATTG GAGGATTCGT ATCACAAAGA 
ATCAAAGTAA TCTCAAAACC ATCGAAAAAG AAGCAAAGTA TGAAAAATAC GGACTGCAAG 
TATCTTTGTA TTGCCAGTGG AACCAAAGTT GCTCTATTCA ATCGTCTCCG GTCACAGACA 
GTCAGTACAA GATACCTTCA TGTTGAGGGA AATGCATTCC ATGCTTCCTC CACGAAATGG 
GGAGCCTTTA CTATTCATCT TTTTGACGAC GAACGTGGTT TGCAAGAGAC TGATAATTTT 
GCAGTTCGTG ATGGATTTGT TTACTATGGA AGTGTCGTTA AGCTTGTTGA TAGTGTTACC 
GGAATCGCAC TGCCAAGATT GAGAATCAGA AAGGTCGATA AGCAGCAAGT AATTCTTGAT 
GCATCGTGTA GTGAAGAACC AGTCTCTCAA CTTCACAAAT GTGCATTCCA AATGATTGAC 
AATGAGCTCG TCTATCTTTG TCTCAGTCAT GACAAGATTA TTCAACATCA AGCCACAGCT 
ATCAATGAGC ATCGTCATCA GATCAACGAT GGAGCTGCGT GGACAATCAT CTCGACTGAT 
AAGGCTGAAT ATCGATTCTT CGAAGCGATG GGACAGGTTG CGAACCCGAT TTCTCCATGT 
CCAGTTGTCG GTTCACTTGA AGTTGATGGA CATGGTGAAG CGTCGCGTGT CGAGTTGCAT 
GGAAGAGATT TTAAGCCAAA TCTGAAAGTT TGGTTTGGAG CAACCCCGGT TGAAACGACG 
TTCAGAAGTG AAGAGAGTCT TCATTGTTCG ATACCACCTG TGAGTCAGGT TCGAAATGAG 
CAAACTCACT GGATGTTTAC GAATAGGACA ACCGGCGATG TTGAGGTTCC AATTTCATTG 
GTACGAGATG ATGGTGTCGT CTACTCGTCG GGTCTCACAT TTTCCTACAA ATCATTGGAA 
CGACATGGAC CGTGCAGAAT TGTGTCCAAT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001023278.1
CDS	4..2019
Translation	MPLAYSTHDNFYESPKTPQPTWDHVHAQFPLGEPARNLDKFIEAMFQSVSPLAGVAAAPSQIAALQQIQALMTFQMQQNNLFPKIDTISKSPTPELASPSAKRMRLSPSTSSHSDVASTSKGTNGQDQSKNSPTNSDPFYTIGQSTPNTSSFLDTSNSSFGAPSTVVANPMTNYQLAFQAKLGSLHSLIGDSVQSLTSDRMIDFLSNKEKYECVISIFHAKVAQKSYGNEKRFFCPPPCIYLIGQGWKLKKDRVAQLYKTLKASAQKDAAIENDPIHEQQATELVAYIGIGSDTSERQQLDFSTGKVRHPGDQRQDPNIYDYCAAKTLYISDSDKRKYFDLNAQFFYGCGMEIGGFVSQRIKVISKPSKKKQSMKNTDCKYLCIASGTKVALFNRLRSQTVSTRYLHVEGNAFHASSTKWGAFTIHLFDDERGLQETDNFAVRDGFVYYGSVVKLVDSVTGIALPRLRIRKVDKQQVILDASCSEEPVSQLHKCAFQMIDNELVYLCLSHDKIIQHQATAINEHRHQINDGAAWTIISTDKAEYRFFEAMGQVANPISPCPVVGSLEVDGHGEASRVELHGRDFKPNLKVWFGATPVETTFRSEESLHCSIPPVSQVRNEQTHWMFTNRTTGDVEVPISLVRDDGVVYSSGLTFSYKSLERHGPCRIVSNY

Target ORF information:

RefSeq Version	NM_001028107.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Lin-12 And Glp-1 phenotype (lag-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028107.4

ORF Insert Sequence:

ATGCCTCTCG CCTACTCAAC ACACGATAAT TTCTACGAAT CGCCGAAAAC ACCGCAGCCA 
ACCTGGGATC ACGTGCACGC ACAGTTTCCA CTGGGTGAAC CCGCACGAAA TCTGGATAAG 
TTTATAGAAG CAATGTTCCA GTCGGTATCG CCGCTGGCCG GTGTCGCTGC AGCGCCGAGC 
CAAATTGCCG CACTTCAACA GATACAAGCG TTGATGACAT TTCAAATGCA GCAGAATAAT 
TTATTTCCCA AAATTGACAC TATATCGAAA TCGCCAACTC CAGAACTCGC ATCTCCATCG 
GCAAAACGAA TGAGACTTTC TCCCAGTACG AGTAGCCACA GTGATGTCGC ATCAACTTCG 
AAAGGAACAA ATGGGCAAGA TCAGTCGAAA AATTCGCCGA CAAATTCTGA TCCATTCTAC 
ACAATCGGGC AGTCAACTCC AAACACATCA TCATTCCTGG ACACTTCAAA TTCAAGTTTT 
GGAGCTCCGT CAACAGTGGT TGCCAATCCG ATGACCAACT ATCAATTGGC ATTTCAAGCG 
AAACTCGGAA GTCTTCACAG TTTGATTGGA GATTCTGTTC AATCGTTAAC AAGCGATCGA 
ATGATTGATT TCTTGTCAAA CAAGGAGAAG TACGAATGCG TCATATCGAT TTTCCATGCC 
AAAGTTGCCC AAAAGTCATA TGGAAATGAG AAAAGATTCT TCTGCCCACC ACCATGCATT 
TATCTTATTG GCCAAGGCTG GAAACTCAAA AAGGATCGTG TCGCTCAACT GTACAAAACA 
CTCAAAGCTT CAGCTCAAAA GGATGCTGCA ATCGAGAATG ATCCGATTCA CGAGCAACAA 
GCCACAGAAC TCGTCGCCTA CATTGGAATC GGATCAGACA CTTCCGAGCG ACAACAACTT 
GATTTTTCAA CGGGAAAAGT TCGACATCCT GGAGATCAGA GGCAGGATCC AAATATTTAT 
GATTATTGTG CCGCGAAGAC TCTGTATATT AGTGATTCGG ACAAGAGAAA ATATTTCGAT 
TTAAATGCTC AATTCTTCTA TGGATGTGGA ATGGAAATTG GAGGATTCGT ATCACAAAGA 
ATCAAAGTAA TCTCAAAACC ATCGAAAAAG AAGCAAAGTA TGAAAAATAC GGACTGCAAG 
TATCTTTGTA TTGCCAGTGG AACCAAAGTT GCTCTATTCA ATCGTCTCCG GTCACAGACA 
GTCAGTACAA GATACCTTCA TGTTGAGGGA AATGCATTCC ATGCTTCCTC CACGAAATGG 
GGAGCCTTTA CTATTCATCT TTTTGACGAC GAACGTGGTT TGCAAGAGAC TGATAATTTT 
GCAGTTCGTG ATGGATTTGT TTACTATGGA AGTGTCGTTA AGCTTGTTGA TAGTGTTACC 
GGAATCGCAC TGCCAAGATT GAGAATCAGA AAGGTCGATA AGCAGCAAGT AATTCTTGAT 
GCATCGTGTA GTGAAGAACC AGTCTCTCAA CTTCACAAAT GTGCATTCCA AATGATTGAC 
AATGAGCTCG TCTATCTTTG TCTCAGTCAT GACAAGATTA TTCAACATCA AGCCACAGCT 
ATCAATGAGC ATCGTCATCA GATCAACGAT GGAGCTGCGT GGACAATCAT CTCGACTGAT 
AAGGCTGAAT ATCGATTCTT CGAAGCGATG GGACAGGTTG CGAACCCGAT TTCTCCATGT 
CCAGTTGTCG GTTCACTTGA AGTTGATGGA CATGGTGAAG CGTCGCGTGT CGAGTTGCAT 
GGAAGAGATT TTAAGCCAAA TCTGAAAGTT TGGTTTGGAG CAACCCCGGT TGAAACGACG 
TTCAGAAGTG AAGAGAGTCT TCATTGTTCG ATACCACCTG TGAGTCAGGT TCGAAATGAG 
CAAACTCACT GGATGTTTAC GAATAGGACA ACCGGCGATG TTGAGGTTCC AATTTCATTG 
GTACGAGATG ATGGTGTCGT CTACTCGTCG GGTCTCACAT TTTCCTACAA ATCATTGGAA 
CGACATGGAC CGTGCAGAAT TGTGTCCAAT TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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lag-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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