fli-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	fli-1
Entrez Gene ID	176215
Full Name	Protein flightless-1 homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066512.5	NP_498913.2	Protein flightless-1 homolog
NM_066512.6	NP_498913.2	Protein flightless-1 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FLII	XP_511321.3
Bos taurus (cattle)	FLII	XP_002695913.2
Rattus norvegicus (Norway rat)	Flii	NP_001008280.1
Drosophila melanogaster (fruit fly)	fliI	NP_525097.1
Homo sapiens (human)	FLII	NP_002009.1
Mus musculus (house mouse)	Flii	NP_071292.1
Danio rerio (zebrafish)	flii	NP_001244075.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011292	XP_309356.4
Caenorhabditis elegans (roundworm)	fli-1	NP_498913.2
Xenopus tropicalis (tropical clawed frog)	flii	NP_001015848.1
Canis lupus familiaris (dog)	FLII	XP_005620209.1
	FLII	XP_001094628.2
Gallus gallus (chicken)	FLII	NP_001025806.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FLI-1 controls germ line morphogenesis and rachis organization
Title: FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans.

fli-1 plays an important role in regulating the actin-dependent events during C. elegans development.
Title: The Flightless I homolog, fli-1, regulates anterior/posterior polarity, asymmetric cell division and ovulation during Caenorhabditis elegans development.

The following fli-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fli-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340751	NM_066512.6 Latest version!	Caenorhabditis elegans Protein flightless-1 homolog (fli-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa340751	NM_066512.5	Caenorhabditis elegans Protein flightless-1 homolog (fli-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fli-1 ( NM_066512.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498913.2 Caenorhabditis elegans Protein flightless-1 homolog (fli-1), partial mRNA.

Price & Availability↑

CloneID	OCa340751
Clone ID Related Accession (Same CDS sequence)	NM_066512.5 , NM_066512.6
Accession Version	NM_066512.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein flightless-1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066512.4. COMPLETENESS: incomplete on both ends.

ATGTCAACAG GAGTGTTACA ATTCGTCAAA GGAATCGATT TCTCGGGAAA TGACTTTTCG 
GGAGATCGAT TTCCTCACGA TGTGGAACAA ATGACGCAAA TGACATGGTT GAAATTGAAC 
GATTCGAAGC TCGAACAGGT GCCTGATGAG CTGTCGCGTT GTGCGAATCT GGAACATCTG 
CAAATGGCTC ACAATCAACT TATTTCTGTA CATGGAGAGC TTTCTGATTT ACCGCGACTA 
CGATCAGTGA TTGTCAGGGA TAATAACCTA AAAACCGCCG GAATTCCAAC GGACATTTTC 
CGCATGAAAG ATCTTACAAT TATCGATTTA TCGCGAAATC AATTGAGAGA AGTTCCAACA 
AATTTGGAGT ATGCAAAAGG ATCAATCGTT CTGAATTTGA GCTATAATAA TATTGAAACT 
ATTCCGAACA GCGTTTGTGC CAATCTTATC GACTTATTGT TCCTGGATCT TTCGAACAAC 
AAATTGGACA TGCTTCCACC ACAGATTCGT CGATTAAGCA TGCTTCAAAG TTTGAAACTT 
TCAAATAATC CACTTAATCA TTTCCAATTG AAACAATTAC CAAGCATGAC ATCCCTAAGC 
GTTCTTCACA TGAGTAATAC CAATCGAACG CTGGATAATA TTCCTCCAAC TCTGGATGAT 
ATGCATAATT TAAGAGATGT TGATTTTTCT GAAAACAATC TTCCCATTGT CCCGGAAGCT 
CTCTTCAAAC TGCGAAATCT TCGAAAATTG AATCTAAGTG GAAATAAAAT CGAAAAATTG 
AATATGACTG AAGGAGAATG GGAGAATTTG GAAACATTAA ACATGAGCCA TAATCAATTG 
ACAGTTCTTC CGGATTGTGT TGTCAAATTG ACAAGACTCA CCAAATTATA CGCAGCAAAT 
AACCAATTGA CATTCGAAGG AATACCATCT GGAATAGGAA AACTCATTCA ACTTACCGTA 
CTTCATTTGT CATATAACAA GCTGGAATTA GTTCCAGAAG GAATCTCGCG ATGTGTTAAG 
CTTCAGAAAT TGAAATTAGA TCATAATCGA TTGATTACAC TGCCAGAGGG AATACATTTG 
TTGCCTGATT TGAAAGTTTT GGATTTACAT GAAAACGAAA ACTTGGTGAT GCCTCCAAAA 
CCGAATGATG CAAGAAAGAA ATTAGCATTT TATAATATTG ATTTCTCGTT GGAACATCAG 
CGTAAAATCG CAGGGCAAAT GACAAGTCCA TCGTCTTCGA TCTCATCAGT TCATTCGGGT 
GGAGCACGAC AAGATGCTTT GGCACGACGG AAAGAATTTA TTCGACGCAG AAAGCAACAG 
GCCGATCAAC AGAGTGCTGA TAAGGTTATA CAGGGAATGT CGAAAATCGC TGGCGTCGGT 
GCGGCTCTTA CGGAGAAGCA ACAAGAGGAA GAAGAACGAC AATTGGAAGC AAAAAGTGCA 
GTTAATTGGA AAAAGAATAT TGAAAAGAAT CGTCGACACA TTGATTACAG TGATATATTT 
GATGAAGATG TTGGTAGTGA CGAAGGAATG TGGGTATGGG AAATTGAGAA TTTCTATCCA 
TCAATTATGG ATGAAGCATT CCATGGGCAA TTTTATGATG CAGATGCTTA CCTGGTACTA 
AAGACGACTC GAGAAGCATC CGGACAACTT CGTCATGCAA TATTTTATTG GCTCGGTGAG 
CACGCTTCTC TTGACAAGGG AATGTGTTCT GCTGTACACG CTGTTGGCCT CCGAAATCAC 
CTGAATGCCA CGTGTCGAAC ACAACGAGAA GAAATGAACG ATGAGACGGA AGAGTTTTTG 
ACGTTATTTG GAGAAGAAAT TGTGTATATT GAGGGTGGAC GGACAATTTC TGGATTCTAT 
ACAACTGAAA AACCAGCTCA TTTAACACGA CTATATCGAG CAGGAGTTAA CGGAACTGCT 
GTAGAAATGG AACCCGTACC GCTCAGTGTC GAATCATTAG ATCCTAGATT TTGCTTTCTT 
TTGGATGCAG GAGAAACTAT TTGGATATGG AGTGGCTATA AATCAAGGAT CACAGTGTCA 
AATAAAGCAA GATTGTTCGC AGAGCGGCTC AACAAGCGTG ATAGGAAAGG AAAGAGTGAA 
ATTGAGACAT GCCGACAAGC TAGATGTCCT CCAGAATTCT GGCAAGCGCT CACTGGAAAT 
CCGGATAAAC CACAAGGAGC TATTGTTGAA CATGTACCGG AAGGATTTGT TGCTGAAAGA 
AAAAAGCTTT ATAAGGTGAA CATTGGAATG GGATTCCTCG AATTACCACA AGTAGAGCTG 
CCCAAGGGAA TTGCAAAACA AGATATGCTT GGATCCAAAG GAGTTTTCGT TCTTGATTCC 
AATTCGGACA TCTTTTTATG GATTGGCAAA AAAGCGAACC GATTACTGAA AATGGCTGGT 
CAGAAATTAG TTGTTGAACT ACATCAAATG ATTGATAGAC CAGATTATGC TCAAGTATAT 
CGAGAAACTG AAGGAGAAGA ATCAATGATG TTCCGATCGA AATTTGCTGG ATGGGATGAA 
ATAGTTCCAG TTGATTACAC GAGAACAAGT GATTCTGTAC AAAGAGTTCC AGATTTGAAG 
GTTATTGTGA AGAAAGATAA TATGATGCGT GCAGATTTGG CAGCTCTATT CCTAGAAAGA 
CAACCTTCAA TGTCATATGA AGAAAGTGAA GAACTAATGG AAGATTGCAA TTATGATTTA 
GAGCTTATGG AGAGTTTTGT ACTTGAGGGA AAGAAATTCG TGAAACTTCC ACAAAAAGAG 
TTTGGAATAT TCTACACAAT GGACTGTTAC GTATTCCTTT GTCGATATGC AGTAATGCCG 
GAAGAGGATG AGGAAGGTGA AGATGAACAC GATGAAGATG ATAAGCCAGA GATGGACTTC 
AAGTGTGTCG TGTACTTTTG GCAAGGAAGA GATGCTTCAA ATATGGGATG GTTGAATTTT 
ACATTCCAGC TTCAACCAAA TTTTGAGGAG ATATTTAAGG ATAAATTGGA AGTTGTTCGA 
ATGTATCAAC AACAAGAGAA TCATAAATTC CTTTCGCATT TCAAACGAAA ATTCCTGATC 
AAGCGTGGTC GACGTGGTTT GACAAAGAAT CTCGGCGGAA AATGGCCAGA ATTATTCCAA 
ATGAGAGCCA ATGGATCAAG TGTATGCAAT AGAACTATTC AAGTTGATTG CCAAGCAAAC 
CAACTATGCT CAGCATTCTG TCACATGCTC CGAATTCCAT TCAAAGAAAT CGAAGAAGAT 
GGTCATCGTG GAGTGGTTTA CGTATGGATG GGTAAAGATT CTGATCCACG TGAACATGAA 
TTTGCTCGAC AAGTAGCATC AGATCTTGTC GTTCGTGACG ATGACAATGA CTTCCGAATT 
GTCGAAGTTC AAGAAGGAGA AGAAAATGAA GAATTCTGGA AAGTTCTCGG TGGAAAGAAA 
AAATATGAAA CTGATAGTTC ATTTGTTAAG CATACAAGAC TGTTTCGATG CACCAACGAG 
AAGGGATACT TTGCGATTTC CGAGAAAACT GTCGATTTTT GTCAGGATGA TCTTGATGAT 
GACGATATAA TGATTCTGGA TAACGGAGAT GCAGTTTTCC TTTGGATTGG AGCAAGATCT 
TCAGATATTG AAGCAAAACT TTCTTATCAA GCCGCTCAAG TATATCATGC ATCAATGAGA 
ATGAAAGCAA ATGAGAAGCC GAGAAAGTTC ATGTTAGCAG TTCGAGGACG AGAAAGTTGC 
AGATTCCGGA AATGCTTCCA TGCATGGTCC AAAATGAAGG AACCAATGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498913.2
CDS	1..3774
Translation	MSTGVLQFVKGIDFSGNDFSGDRFPHDVEQMTQMTWLKLNDSKLEQVPDELSRCANLEHLQMAHNQLISVHGELSDLPRLRSVIVRDNNLKTAGIPTDIFRMKDLTIIDLSRNQLREVPTNLEYAKGSIVLNLSYNNIETIPNSVCANLIDLLFLDLSNNKLDMLPPQIRRLSMLQSLKLSNNPLNHFQLKQLPSMTSLSVLHMSNTNRTLDNIPPTLDDMHNLRDVDFSENNLPIVPEALFKLRNLRKLNLSGNKIEKLNMTEGEWENLETLNMSHNQLTVLPDCVVKLTRLTKLYAANNQLTFEGIPSGIGKLIQLTVLHLSYNKLELVPEGISRCVKLQKLKLDHNRLITLPEGIHLLPDLKVLDLHENENLVMPPKPNDARKKLAFYNIDFSLEHQRKIAGQMTSPSSSISSVHSGGARQDALARRKEFIRRRKQQADQQSADKVIQGMSKIAGVGAALTEKQQEEEERQLEAKSAVNWKKNIEKNRRHIDYSDIFDEDVGSDEGMWVWEIENFYPSIMDEAFHGQFYDADAYLVLKTTREASGQLRHAIFYWLGEHASLDKGMCSAVHAVGLRNHLNATCRTQREEMNDETEEFLTLFGEEIVYIEGGRTISGFYTTEKPAHLTRLYRAGVNGTAVEMEPVPLSVESLDPRFCFLLDAGETIWIWSGYKSRITVSNKARLFAERLNKRDRKGKSEIETCRQARCPPEFWQALTGNPDKPQGAIVEHVPEGFVAERKKLYKVNIGMGFLELPQVELPKGIAKQDMLGSKGVFVLDSNSDIFLWIGKKANRLLKMAGQKLVVELHQMIDRPDYAQVYRETEGEESMMFRSKFAGWDEIVPVDYTRTSDSVQRVPDLKVIVKKDNMMRADLAALFLERQPSMSYEESEELMEDCNYDLELMESFVLEGKKFVKLPQKEFGIFYTMDCYVFLCRYAVMPEEDEEGEDEHDEDDKPEMDFKCVVYFWQGRDASNMGWLNFTFQLQPNFEEIFKDKLEVVRMYQQQENHKFLSHFKRKFLIKRGRRGLTKNLGGKWPELFQMRANGSSVCNRTIQVDCQANQLCSAFCHMLRIPFKEIEEDGHRGVVYVWMGKDSDPREHEFARQVASDLVVRDDDNDFRIVEVQEGEENEEFWKVLGGKKKYETDSSFVKHTRLFRCTNEKGYFAISEKTVDFCQDDLDDDDIMILDNGDAVFLWIGARSSDIEAKLSYQAAQVYHASMRMKANEKPRKFMLAVRGRESCRFRKCFHAWSKMKEPMG

Target ORF information:

RefSeq Version	NM_066512.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein flightless-1 homolog (fli-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066512.5

ORF Insert Sequence:

ATGTCAACAG GAGTGTTACA ATTCGTCAAA GGAATCGATT TCTCGGGAAA TGACTTTTCG 
GGAGATCGAT TTCCTCACGA TGTGGAACAA ATGACGCAAA TGACATGGTT GAAATTGAAC 
GATTCGAAGC TCGAACAGGT GCCTGATGAG CTGTCGCGTT GTGCGAATCT GGAACATCTG 
CAAATGGCTC ACAATCAACT TATTTCTGTA CATGGAGAGC TTTCTGATTT ACCGCGACTA 
CGATCAGTGA TTGTCAGGGA TAATAACCTA AAAACCGCCG GAATTCCAAC GGACATTTTC 
CGCATGAAAG ATCTTACAAT TATCGATTTA TCGCGAAATC AATTGAGAGA AGTTCCAACA 
AATTTGGAGT ATGCAAAAGG ATCAATCGTT CTGAATTTGA GCTATAATAA TATTGAAACT 
ATTCCGAACA GCGTTTGTGC CAATCTTATC GACTTATTGT TCCTGGATCT TTCGAACAAC 
AAATTGGACA TGCTTCCACC ACAGATTCGT CGATTAAGCA TGCTTCAAAG TTTGAAACTT 
TCAAATAATC CACTTAATCA TTTCCAATTG AAACAATTAC CAAGCATGAC ATCCCTAAGC 
GTTCTTCACA TGAGTAATAC CAATCGAACG CTGGATAATA TTCCTCCAAC TCTGGATGAT 
ATGCATAATT TAAGAGATGT TGATTTTTCT GAAAACAATC TTCCCATTGT CCCGGAAGCT 
CTCTTCAAAC TGCGAAATCT TCGAAAATTG AATCTAAGTG GAAATAAAAT CGAAAAATTG 
AATATGACTG AAGGAGAATG GGAGAATTTG GAAACATTAA ACATGAGCCA TAATCAATTG 
ACAGTTCTTC CGGATTGTGT TGTCAAATTG ACAAGACTCA CCAAATTATA CGCAGCAAAT 
AACCAATTGA CATTCGAAGG AATACCATCT GGAATAGGAA AACTCATTCA ACTTACCGTA 
CTTCATTTGT CATATAACAA GCTGGAATTA GTTCCAGAAG GAATCTCGCG ATGTGTTAAG 
CTTCAGAAAT TGAAATTAGA TCATAATCGA TTGATTACAC TGCCAGAGGG AATACATTTG 
TTGCCTGATT TGAAAGTTTT GGATTTACAT GAAAACGAAA ACTTGGTGAT GCCTCCAAAA 
CCGAATGATG CAAGAAAGAA ATTAGCATTT TATAATATTG ATTTCTCGTT GGAACATCAG 
CGTAAAATCG CAGGGCAAAT GACAAGTCCA TCGTCTTCGA TCTCATCAGT TCATTCGGGT 
GGAGCACGAC AAGATGCTTT GGCACGACGG AAAGAATTTA TTCGACGCAG AAAGCAACAG 
GCCGATCAAC AGAGTGCTGA TAAGGTTATA CAGGGAATGT CGAAAATCGC TGGCGTCGGT 
GCGGCTCTTA CGGAGAAGCA ACAAGAGGAA GAAGAACGAC AATTGGAAGC AAAAAGTGCA 
GTTAATTGGA AAAAGAATAT TGAAAAGAAT CGTCGACACA TTGATTACAG TGATATATTT 
GATGAAGATG TTGGTAGTGA CGAAGGAATG TGGGTATGGG AAATTGAGAA TTTCTATCCA 
TCAATTATGG ATGAAGCATT CCATGGGCAA TTTTATGATG CAGATGCTTA CCTGGTACTA 
AAGACGACTC GAGAAGCATC CGGACAACTT CGTCATGCAA TATTTTATTG GCTCGGTGAG 
CACGCTTCTC TTGACAAGGG AATGTGTTCT GCTGTACACG CTGTTGGCCT CCGAAATCAC 
CTGAATGCCA CGTGTCGAAC ACAACGAGAA GAAATGAACG ATGAGACGGA AGAGTTTTTG 
ACGTTATTTG GAGAAGAAAT TGTGTATATT GAGGGTGGAC GGACAATTTC TGGATTCTAT 
ACAACTGAAA AACCAGCTCA TTTAACACGA CTATATCGAG CAGGAGTTAA CGGAACTGCT 
GTAGAAATGG AACCCGTACC GCTCAGTGTC GAATCATTAG ATCCTAGATT TTGCTTTCTT 
TTGGATGCAG GAGAAACTAT TTGGATATGG AGTGGCTATA AATCAAGGAT CACAGTGTCA 
AATAAAGCAA GATTGTTCGC AGAGCGGCTC AACAAGCGTG ATAGGAAAGG AAAGAGTGAA 
ATTGAGACAT GCCGACAAGC TAGATGTCCT CCAGAATTCT GGCAAGCGCT CACTGGAAAT 
CCGGATAAAC CACAAGGAGC TATTGTTGAA CATGTACCGG AAGGATTTGT TGCTGAAAGA 
AAAAAGCTTT ATAAGGTGAA CATTGGAATG GGATTCCTCG AATTACCACA AGTAGAGCTG 
CCCAAGGGAA TTGCAAAACA AGATATGCTT GGATCCAAAG GAGTTTTCGT TCTTGATTCC 
AATTCGGACA TCTTTTTATG GATTGGCAAA AAAGCGAACC GATTACTGAA AATGGCTGGT 
CAGAAATTAG TTGTTGAACT ACATCAAATG ATTGATAGAC CAGATTATGC TCAAGTATAT 
CGAGAAACTG AAGGAGAAGA ATCAATGATG TTCCGATCGA AATTTGCTGG ATGGGATGAA 
ATAGTTCCAG TTGATTACAC GAGAACAAGT GATTCTGTAC AAAGAGTTCC AGATTTGAAG 
GTTATTGTGA AGAAAGATAA TATGATGCGT GCAGATTTGG CAGCTCTATT CCTAGAAAGA 
CAACCTTCAA TGTCATATGA AGAAAGTGAA GAACTAATGG AAGATTGCAA TTATGATTTA 
GAGCTTATGG AGAGTTTTGT ACTTGAGGGA AAGAAATTCG TGAAACTTCC ACAAAAAGAG 
TTTGGAATAT TCTACACAAT GGACTGTTAC GTATTCCTTT GTCGATATGC AGTAATGCCG 
GAAGAGGATG AGGAAGGTGA AGATGAACAC GATGAAGATG ATAAGCCAGA GATGGACTTC 
AAGTGTGTCG TGTACTTTTG GCAAGGAAGA GATGCTTCAA ATATGGGATG GTTGAATTTT 
ACATTCCAGC TTCAACCAAA TTTTGAGGAG ATATTTAAGG ATAAATTGGA AGTTGTTCGA 
ATGTATCAAC AACAAGAGAA TCATAAATTC CTTTCGCATT TCAAACGAAA ATTCCTGATC 
AAGCGTGGTC GACGTGGTTT GACAAAGAAT CTCGGCGGAA AATGGCCAGA ATTATTCCAA 
ATGAGAGCCA ATGGATCAAG TGTATGCAAT AGAACTATTC AAGTTGATTG CCAAGCAAAC 
CAACTATGCT CAGCATTCTG TCACATGCTC CGAATTCCAT TCAAAGAAAT CGAAGAAGAT 
GGTCATCGTG GAGTGGTTTA CGTATGGATG GGTAAAGATT CTGATCCACG TGAACATGAA 
TTTGCTCGAC AAGTAGCATC AGATCTTGTC GTTCGTGACG ATGACAATGA CTTCCGAATT 
GTCGAAGTTC AAGAAGGAGA AGAAAATGAA GAATTCTGGA AAGTTCTCGG TGGAAAGAAA 
AAATATGAAA CTGATAGTTC ATTTGTTAAG CATACAAGAC TGTTTCGATG CACCAACGAG 
AAGGGATACT TTGCGATTTC CGAGAAAACT GTCGATTTTT GTCAGGATGA TCTTGATGAT 
GACGATATAA TGATTCTGGA TAACGGAGAT GCAGTTTTCC TTTGGATTGG AGCAAGATCT 
TCAGATATTG AAGCAAAACT TTCTTATCAA GCCGCTCAAG TATATCATGC ATCAATGAGA 
ATGAAAGCAA ATGAGAAGCC GAGAAAGTTC ATGTTAGCAG TTCGAGGACG AGAAAGTTGC 
AGATTCCGGA AATGCTTCCA TGCATGGTCC AAAATGAAGG AACCAATGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fli-1 ( NM_066512.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498913.2 Caenorhabditis elegans Protein flightless-1 homolog (fli-1), mRNA.

Price & Availability↑

CloneID	OCa340751
Clone ID Related Accession (Same CDS sequence)	NM_066512.5 , NM_066512.6
Accession Version	NM_066512.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein flightless-1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066512.5.

ATGTCAACAG GAGTGTTACA ATTCGTCAAA GGAATCGATT TCTCGGGAAA TGACTTTTCG 
GGAGATCGAT TTCCTCACGA TGTGGAACAA ATGACGCAAA TGACATGGTT GAAATTGAAC 
GATTCGAAGC TCGAACAGGT GCCTGATGAG CTGTCGCGTT GTGCGAATCT GGAACATCTG 
CAAATGGCTC ACAATCAACT TATTTCTGTA CATGGAGAGC TTTCTGATTT ACCGCGACTA 
CGATCAGTGA TTGTCAGGGA TAATAACCTA AAAACCGCCG GAATTCCAAC GGACATTTTC 
CGCATGAAAG ATCTTACAAT TATCGATTTA TCGCGAAATC AATTGAGAGA AGTTCCAACA 
AATTTGGAGT ATGCAAAAGG ATCAATCGTT CTGAATTTGA GCTATAATAA TATTGAAACT 
ATTCCGAACA GCGTTTGTGC CAATCTTATC GACTTATTGT TCCTGGATCT TTCGAACAAC 
AAATTGGACA TGCTTCCACC ACAGATTCGT CGATTAAGCA TGCTTCAAAG TTTGAAACTT 
TCAAATAATC CACTTAATCA TTTCCAATTG AAACAATTAC CAAGCATGAC ATCCCTAAGC 
GTTCTTCACA TGAGTAATAC CAATCGAACG CTGGATAATA TTCCTCCAAC TCTGGATGAT 
ATGCATAATT TAAGAGATGT TGATTTTTCT GAAAACAATC TTCCCATTGT CCCGGAAGCT 
CTCTTCAAAC TGCGAAATCT TCGAAAATTG AATCTAAGTG GAAATAAAAT CGAAAAATTG 
AATATGACTG AAGGAGAATG GGAGAATTTG GAAACATTAA ACATGAGCCA TAATCAATTG 
ACAGTTCTTC CGGATTGTGT TGTCAAATTG ACAAGACTCA CCAAATTATA CGCAGCAAAT 
AACCAATTGA CATTCGAAGG AATACCATCT GGAATAGGAA AACTCATTCA ACTTACCGTA 
CTTCATTTGT CATATAACAA GCTGGAATTA GTTCCAGAAG GAATCTCGCG ATGTGTTAAG 
CTTCAGAAAT TGAAATTAGA TCATAATCGA TTGATTACAC TGCCAGAGGG AATACATTTG 
TTGCCTGATT TGAAAGTTTT GGATTTACAT GAAAACGAAA ACTTGGTGAT GCCTCCAAAA 
CCGAATGATG CAAGAAAGAA ATTAGCATTT TATAATATTG ATTTCTCGTT GGAACATCAG 
CGTAAAATCG CAGGGCAAAT GACAAGTCCA TCGTCTTCGA TCTCATCAGT TCATTCGGGT 
GGAGCACGAC AAGATGCTTT GGCACGACGG AAAGAATTTA TTCGACGCAG AAAGCAACAG 
GCCGATCAAC AGAGTGCTGA TAAGGTTATA CAGGGAATGT CGAAAATCGC TGGCGTCGGT 
GCGGCTCTTA CGGAGAAGCA ACAAGAGGAA GAAGAACGAC AATTGGAAGC AAAAAGTGCA 
GTTAATTGGA AAAAGAATAT TGAAAAGAAT CGTCGACACA TTGATTACAG TGATATATTT 
GATGAAGATG TTGGTAGTGA CGAAGGAATG TGGGTATGGG AAATTGAGAA TTTCTATCCA 
TCAATTATGG ATGAAGCATT CCATGGGCAA TTTTATGATG CAGATGCTTA CCTGGTACTA 
AAGACGACTC GAGAAGCATC CGGACAACTT CGTCATGCAA TATTTTATTG GCTCGGTGAG 
CACGCTTCTC TTGACAAGGG AATGTGTTCT GCTGTACACG CTGTTGGCCT CCGAAATCAC 
CTGAATGCCA CGTGTCGAAC ACAACGAGAA GAAATGAACG ATGAGACGGA AGAGTTTTTG 
ACGTTATTTG GAGAAGAAAT TGTGTATATT GAGGGTGGAC GGACAATTTC TGGATTCTAT 
ACAACTGAAA AACCAGCTCA TTTAACACGA CTATATCGAG CAGGAGTTAA CGGAACTGCT 
GTAGAAATGG AACCCGTACC GCTCAGTGTC GAATCATTAG ATCCTAGATT TTGCTTTCTT 
TTGGATGCAG GAGAAACTAT TTGGATATGG AGTGGCTATA AATCAAGGAT CACAGTGTCA 
AATAAAGCAA GATTGTTCGC AGAGCGGCTC AACAAGCGTG ATAGGAAAGG AAAGAGTGAA 
ATTGAGACAT GCCGACAAGC TAGATGTCCT CCAGAATTCT GGCAAGCGCT CACTGGAAAT 
CCGGATAAAC CACAAGGAGC TATTGTTGAA CATGTACCGG AAGGATTTGT TGCTGAAAGA 
AAAAAGCTTT ATAAGGTGAA CATTGGAATG GGATTCCTCG AATTACCACA AGTAGAGCTG 
CCCAAGGGAA TTGCAAAACA AGATATGCTT GGATCCAAAG GAGTTTTCGT TCTTGATTCC 
AATTCGGACA TCTTTTTATG GATTGGCAAA AAAGCGAACC GATTACTGAA AATGGCTGGT 
CAGAAATTAG TTGTTGAACT ACATCAAATG ATTGATAGAC CAGATTATGC TCAAGTATAT 
CGAGAAACTG AAGGAGAAGA ATCAATGATG TTCCGATCGA AATTTGCTGG ATGGGATGAA 
ATAGTTCCAG TTGATTACAC GAGAACAAGT GATTCTGTAC AAAGAGTTCC AGATTTGAAG 
GTTATTGTGA AGAAAGATAA TATGATGCGT GCAGATTTGG CAGCTCTATT CCTAGAAAGA 
CAACCTTCAA TGTCATATGA AGAAAGTGAA GAACTAATGG AAGATTGCAA TTATGATTTA 
GAGCTTATGG AGAGTTTTGT ACTTGAGGGA AAGAAATTCG TGAAACTTCC ACAAAAAGAG 
TTTGGAATAT TCTACACAAT GGACTGTTAC GTATTCCTTT GTCGATATGC AGTAATGCCG 
GAAGAGGATG AGGAAGGTGA AGATGAACAC GATGAAGATG ATAAGCCAGA GATGGACTTC 
AAGTGTGTCG TGTACTTTTG GCAAGGAAGA GATGCTTCAA ATATGGGATG GTTGAATTTT 
ACATTCCAGC TTCAACCAAA TTTTGAGGAG ATATTTAAGG ATAAATTGGA AGTTGTTCGA 
ATGTATCAAC AACAAGAGAA TCATAAATTC CTTTCGCATT TCAAACGAAA ATTCCTGATC 
AAGCGTGGTC GACGTGGTTT GACAAAGAAT CTCGGCGGAA AATGGCCAGA ATTATTCCAA 
ATGAGAGCCA ATGGATCAAG TGTATGCAAT AGAACTATTC AAGTTGATTG CCAAGCAAAC 
CAACTATGCT CAGCATTCTG TCACATGCTC CGAATTCCAT TCAAAGAAAT CGAAGAAGAT 
GGTCATCGTG GAGTGGTTTA CGTATGGATG GGTAAAGATT CTGATCCACG TGAACATGAA 
TTTGCTCGAC AAGTAGCATC AGATCTTGTC GTTCGTGACG ATGACAATGA CTTCCGAATT 
GTCGAAGTTC AAGAAGGAGA AGAAAATGAA GAATTCTGGA AAGTTCTCGG TGGAAAGAAA 
AAATATGAAA CTGATAGTTC ATTTGTTAAG CATACAAGAC TGTTTCGATG CACCAACGAG 
AAGGGATACT TTGCGATTTC CGAGAAAACT GTCGATTTTT GTCAGGATGA TCTTGATGAT 
GACGATATAA TGATTCTGGA TAACGGAGAT GCAGTTTTCC TTTGGATTGG AGCAAGATCT 
TCAGATATTG AAGCAAAACT TTCTTATCAA GCCGCTCAAG TATATCATGC ATCAATGAGA 
ATGAAAGCAA ATGAGAAGCC GAGAAAGTTC ATGTTAGCAG TTCGAGGACG AGAAAGTTGC 
AGATTCCGGA AATGCTTCCA TGCATGGTCC AAAATGAAGG AACCAATGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498913.2
CDS	44..3817
Translation	MSTGVLQFVKGIDFSGNDFSGDRFPHDVEQMTQMTWLKLNDSKLEQVPDELSRCANLEHLQMAHNQLISVHGELSDLPRLRSVIVRDNNLKTAGIPTDIFRMKDLTIIDLSRNQLREVPTNLEYAKGSIVLNLSYNNIETIPNSVCANLIDLLFLDLSNNKLDMLPPQIRRLSMLQSLKLSNNPLNHFQLKQLPSMTSLSVLHMSNTNRTLDNIPPTLDDMHNLRDVDFSENNLPIVPEALFKLRNLRKLNLSGNKIEKLNMTEGEWENLETLNMSHNQLTVLPDCVVKLTRLTKLYAANNQLTFEGIPSGIGKLIQLTVLHLSYNKLELVPEGISRCVKLQKLKLDHNRLITLPEGIHLLPDLKVLDLHENENLVMPPKPNDARKKLAFYNIDFSLEHQRKIAGQMTSPSSSISSVHSGGARQDALARRKEFIRRRKQQADQQSADKVIQGMSKIAGVGAALTEKQQEEEERQLEAKSAVNWKKNIEKNRRHIDYSDIFDEDVGSDEGMWVWEIENFYPSIMDEAFHGQFYDADAYLVLKTTREASGQLRHAIFYWLGEHASLDKGMCSAVHAVGLRNHLNATCRTQREEMNDETEEFLTLFGEEIVYIEGGRTISGFYTTEKPAHLTRLYRAGVNGTAVEMEPVPLSVESLDPRFCFLLDAGETIWIWSGYKSRITVSNKARLFAERLNKRDRKGKSEIETCRQARCPPEFWQALTGNPDKPQGAIVEHVPEGFVAERKKLYKVNIGMGFLELPQVELPKGIAKQDMLGSKGVFVLDSNSDIFLWIGKKANRLLKMAGQKLVVELHQMIDRPDYAQVYRETEGEESMMFRSKFAGWDEIVPVDYTRTSDSVQRVPDLKVIVKKDNMMRADLAALFLERQPSMSYEESEELMEDCNYDLELMESFVLEGKKFVKLPQKEFGIFYTMDCYVFLCRYAVMPEEDEEGEDEHDEDDKPEMDFKCVVYFWQGRDASNMGWLNFTFQLQPNFEEIFKDKLEVVRMYQQQENHKFLSHFKRKFLIKRGRRGLTKNLGGKWPELFQMRANGSSVCNRTIQVDCQANQLCSAFCHMLRIPFKEIEEDGHRGVVYVWMGKDSDPREHEFARQVASDLVVRDDDNDFRIVEVQEGEENEEFWKVLGGKKKYETDSSFVKHTRLFRCTNEKGYFAISEKTVDFCQDDLDDDDIMILDNGDAVFLWIGARSSDIEAKLSYQAAQVYHASMRMKANEKPRKFMLAVRGRESCRFRKCFHAWSKMKEPMG

Target ORF information:

RefSeq Version	NM_066512.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein flightless-1 homolog (fli-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066512.6

ORF Insert Sequence:

ATGTCAACAG GAGTGTTACA ATTCGTCAAA GGAATCGATT TCTCGGGAAA TGACTTTTCG 
GGAGATCGAT TTCCTCACGA TGTGGAACAA ATGACGCAAA TGACATGGTT GAAATTGAAC 
GATTCGAAGC TCGAACAGGT GCCTGATGAG CTGTCGCGTT GTGCGAATCT GGAACATCTG 
CAAATGGCTC ACAATCAACT TATTTCTGTA CATGGAGAGC TTTCTGATTT ACCGCGACTA 
CGATCAGTGA TTGTCAGGGA TAATAACCTA AAAACCGCCG GAATTCCAAC GGACATTTTC 
CGCATGAAAG ATCTTACAAT TATCGATTTA TCGCGAAATC AATTGAGAGA AGTTCCAACA 
AATTTGGAGT ATGCAAAAGG ATCAATCGTT CTGAATTTGA GCTATAATAA TATTGAAACT 
ATTCCGAACA GCGTTTGTGC CAATCTTATC GACTTATTGT TCCTGGATCT TTCGAACAAC 
AAATTGGACA TGCTTCCACC ACAGATTCGT CGATTAAGCA TGCTTCAAAG TTTGAAACTT 
TCAAATAATC CACTTAATCA TTTCCAATTG AAACAATTAC CAAGCATGAC ATCCCTAAGC 
GTTCTTCACA TGAGTAATAC CAATCGAACG CTGGATAATA TTCCTCCAAC TCTGGATGAT 
ATGCATAATT TAAGAGATGT TGATTTTTCT GAAAACAATC TTCCCATTGT CCCGGAAGCT 
CTCTTCAAAC TGCGAAATCT TCGAAAATTG AATCTAAGTG GAAATAAAAT CGAAAAATTG 
AATATGACTG AAGGAGAATG GGAGAATTTG GAAACATTAA ACATGAGCCA TAATCAATTG 
ACAGTTCTTC CGGATTGTGT TGTCAAATTG ACAAGACTCA CCAAATTATA CGCAGCAAAT 
AACCAATTGA CATTCGAAGG AATACCATCT GGAATAGGAA AACTCATTCA ACTTACCGTA 
CTTCATTTGT CATATAACAA GCTGGAATTA GTTCCAGAAG GAATCTCGCG ATGTGTTAAG 
CTTCAGAAAT TGAAATTAGA TCATAATCGA TTGATTACAC TGCCAGAGGG AATACATTTG 
TTGCCTGATT TGAAAGTTTT GGATTTACAT GAAAACGAAA ACTTGGTGAT GCCTCCAAAA 
CCGAATGATG CAAGAAAGAA ATTAGCATTT TATAATATTG ATTTCTCGTT GGAACATCAG 
CGTAAAATCG CAGGGCAAAT GACAAGTCCA TCGTCTTCGA TCTCATCAGT TCATTCGGGT 
GGAGCACGAC AAGATGCTTT GGCACGACGG AAAGAATTTA TTCGACGCAG AAAGCAACAG 
GCCGATCAAC AGAGTGCTGA TAAGGTTATA CAGGGAATGT CGAAAATCGC TGGCGTCGGT 
GCGGCTCTTA CGGAGAAGCA ACAAGAGGAA GAAGAACGAC AATTGGAAGC AAAAAGTGCA 
GTTAATTGGA AAAAGAATAT TGAAAAGAAT CGTCGACACA TTGATTACAG TGATATATTT 
GATGAAGATG TTGGTAGTGA CGAAGGAATG TGGGTATGGG AAATTGAGAA TTTCTATCCA 
TCAATTATGG ATGAAGCATT CCATGGGCAA TTTTATGATG CAGATGCTTA CCTGGTACTA 
AAGACGACTC GAGAAGCATC CGGACAACTT CGTCATGCAA TATTTTATTG GCTCGGTGAG 
CACGCTTCTC TTGACAAGGG AATGTGTTCT GCTGTACACG CTGTTGGCCT CCGAAATCAC 
CTGAATGCCA CGTGTCGAAC ACAACGAGAA GAAATGAACG ATGAGACGGA AGAGTTTTTG 
ACGTTATTTG GAGAAGAAAT TGTGTATATT GAGGGTGGAC GGACAATTTC TGGATTCTAT 
ACAACTGAAA AACCAGCTCA TTTAACACGA CTATATCGAG CAGGAGTTAA CGGAACTGCT 
GTAGAAATGG AACCCGTACC GCTCAGTGTC GAATCATTAG ATCCTAGATT TTGCTTTCTT 
TTGGATGCAG GAGAAACTAT TTGGATATGG AGTGGCTATA AATCAAGGAT CACAGTGTCA 
AATAAAGCAA GATTGTTCGC AGAGCGGCTC AACAAGCGTG ATAGGAAAGG AAAGAGTGAA 
ATTGAGACAT GCCGACAAGC TAGATGTCCT CCAGAATTCT GGCAAGCGCT CACTGGAAAT 
CCGGATAAAC CACAAGGAGC TATTGTTGAA CATGTACCGG AAGGATTTGT TGCTGAAAGA 
AAAAAGCTTT ATAAGGTGAA CATTGGAATG GGATTCCTCG AATTACCACA AGTAGAGCTG 
CCCAAGGGAA TTGCAAAACA AGATATGCTT GGATCCAAAG GAGTTTTCGT TCTTGATTCC 
AATTCGGACA TCTTTTTATG GATTGGCAAA AAAGCGAACC GATTACTGAA AATGGCTGGT 
CAGAAATTAG TTGTTGAACT ACATCAAATG ATTGATAGAC CAGATTATGC TCAAGTATAT 
CGAGAAACTG AAGGAGAAGA ATCAATGATG TTCCGATCGA AATTTGCTGG ATGGGATGAA 
ATAGTTCCAG TTGATTACAC GAGAACAAGT GATTCTGTAC AAAGAGTTCC AGATTTGAAG 
GTTATTGTGA AGAAAGATAA TATGATGCGT GCAGATTTGG CAGCTCTATT CCTAGAAAGA 
CAACCTTCAA TGTCATATGA AGAAAGTGAA GAACTAATGG AAGATTGCAA TTATGATTTA 
GAGCTTATGG AGAGTTTTGT ACTTGAGGGA AAGAAATTCG TGAAACTTCC ACAAAAAGAG 
TTTGGAATAT TCTACACAAT GGACTGTTAC GTATTCCTTT GTCGATATGC AGTAATGCCG 
GAAGAGGATG AGGAAGGTGA AGATGAACAC GATGAAGATG ATAAGCCAGA GATGGACTTC 
AAGTGTGTCG TGTACTTTTG GCAAGGAAGA GATGCTTCAA ATATGGGATG GTTGAATTTT 
ACATTCCAGC TTCAACCAAA TTTTGAGGAG ATATTTAAGG ATAAATTGGA AGTTGTTCGA 
ATGTATCAAC AACAAGAGAA TCATAAATTC CTTTCGCATT TCAAACGAAA ATTCCTGATC 
AAGCGTGGTC GACGTGGTTT GACAAAGAAT CTCGGCGGAA AATGGCCAGA ATTATTCCAA 
ATGAGAGCCA ATGGATCAAG TGTATGCAAT AGAACTATTC AAGTTGATTG CCAAGCAAAC 
CAACTATGCT CAGCATTCTG TCACATGCTC CGAATTCCAT TCAAAGAAAT CGAAGAAGAT 
GGTCATCGTG GAGTGGTTTA CGTATGGATG GGTAAAGATT CTGATCCACG TGAACATGAA 
TTTGCTCGAC AAGTAGCATC AGATCTTGTC GTTCGTGACG ATGACAATGA CTTCCGAATT 
GTCGAAGTTC AAGAAGGAGA AGAAAATGAA GAATTCTGGA AAGTTCTCGG TGGAAAGAAA 
AAATATGAAA CTGATAGTTC ATTTGTTAAG CATACAAGAC TGTTTCGATG CACCAACGAG 
AAGGGATACT TTGCGATTTC CGAGAAAACT GTCGATTTTT GTCAGGATGA TCTTGATGAT 
GACGATATAA TGATTCTGGA TAACGGAGAT GCAGTTTTCC TTTGGATTGG AGCAAGATCT 
TCAGATATTG AAGCAAAACT TTCTTATCAA GCCGCTCAAG TATATCATGC ATCAATGAGA 
ATGAAAGCAA ATGAGAAGCC GAGAAAGTTC ATGTTAGCAG TTCGAGGACG AGAAAGTTGC 
AGATTCCGGA AATGCTTCCA TGCATGGTCC AAAATGAAGG AACCAATGGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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fli-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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