mpk-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	mpk-1
Entrez Gene ID	175545
Full Name	Mitogen-activated protein kinase mpk-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001027412.3	NP_001022583.1	Mitogen-activated protein kinase mpk-1
NM_001027412.4	NP_001022583.1	Mitogen-activated protein kinase mpk-1
NM_001027413.3	NP_001022584.1	Mitogen-activated protein kinase mpk-1
NM_001027413.2	NP_001022584.1	Mitogen-activated protein kinase mpk-1

Pathways from BioSystems

Homologies

	MAPK1	XP_001089600.2
Bos taurus (cattle)	MAPK1	NP_786987.1
Gallus gallus (chicken)	MAPK1	NP_989481.1
Caenorhabditis elegans (roundworm)	mpk-1	NP_001022583.1
Oryza sativa (rice)	Os06g0154500	NP_001056846.1
Homo sapiens (human)	MAPK1	NP_002736.3
Canis lupus familiaris (dog)	MAPK1	NP_001104270.1
Rattus norvegicus (Norway rat)	Mapk1	NP_446294.1
Danio rerio (zebrafish)	mapk1	NP_878308.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009207	XP_319983.4
Xenopus tropicalis (tropical clawed frog)	mapk1	NP_001017127.1
Mus musculus (house mouse)	Mapk1	NP_001033752.1
Drosophila melanogaster (fruit fly)	rl	NP_001015122.1
Saccharomyces cerevisiae (baker's yeast)	KSS1	NP_011554.3
Eremothecium gossypii	AGOS_ACL191C	NP_983213.1
Arabidopsis thaliana (thale cress)	MPK6	NP_181907.1
Pan troglodytes (chimpanzee)	MAPK1	XP_515005.2
Kluyveromyces lactis	KLLA0A02497g	XP_451110.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that the MPK-1/ERK regulatory network, including FBF-1, FBF-2, and LIP-1, controls the number of sperm by regulating the timing of the sperm-oocyte switch in C. elegans.
Title: MPK-1/ERK regulatory network controls the number of sperm by regulating timing of sperm-oocyte switch in C.?elegans germline.

data suggest that KIN-18 coordinates meiotic progression by modulating the timing of MPK-1 activation and the progression of recombination events
Title: Coordination of Recombination with Meiotic Progression in the Caenorhabditis elegans Germline by KIN-18, a TAO Kinase That Regulates the Timing of MPK-1 Signaling.

We show that MPK-1 signaling plays important developmental roles in pachytene progression and in regulating CEP-1 expression in pachytene, and a possible direct role in DNA damage induced apoptosis
Title: Regulation of Caenorhabditis elegans p53/CEP-1-dependent germ cell apoptosis by Ras/MAPK signaling.

In the proximal germline, sperm-dependent physiological MPK-1 activation results in phosphorylation-based inactivation of NOS-3, FEM-CUL-2-mediated degradation of TRA-1 and the promotion of membrane organization during oogenesis.
Title: MPK-1 ERK controls membrane organization in C. elegans oogenesis via a sex-determination module.

C. elegans ERK kinase MPK-1 or ERK phosphorylating/activating kinase MEK-2 constitutively activates native ClC anion channel CLH-3b.
Title: C. elegans STK39/SPAK ortholog-mediated inhibition of ClC anion channel activity is regulated by WNK-independent ERK kinase signaling.

The following mpk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mpk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340695	NM_001027413.3 Latest version!	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCa341997	NM_001027412.4 Latest version!	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCa341997	NM_001027412.3	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa340695	NM_001027413.2	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mpk-1 ( NM_001027413.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022584.1 Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), mRNA.

Price & Availability↑

CloneID	OCa340695
Clone ID Related Accession (Same CDS sequence)	NM_001027413.3 , NM_001027413.2
Accession Version	NM_001027413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Mitogen-activated protein kinase mpk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001027413.2.

ATGCCAACGT GGATACCTAA CAATTTGTGC GCACAGCCGA CAACGAGAAA TGCTAAACCA 
CCATCGAACG GGCATCCACA AGCGACACAA CAACAATCTG CTCCGGGATC ATTGGCATAC 
CGTAATTCGA GCAACATACC GAATGGAGCA ACCAACCACG TCAGACAACA GAAATGGCAG 
TACACTCGTT CAGGACATAG AAAGATGGCC GACGGAGAAG CGGTTATCTC GACGGTCAAC 
AATGTCGAGG AAGTTCATGG GCAACTTTTT GAGGTTGCTC CCCGTTATGT GAATCTTTCA 
TATATTGGAG AAGGTGCTTA CGGGATGGTC GCCTCTGCAC TTGACACAAT TACTCGTGAT 
CGCGTTGCTA TCAAAAAGAT TTCTCCATTC GAACATCAGA CATTCTGTCA ACGGACACTT 
CGGGAAATCA AAATTCTTAA TCGATTCAAG CATGAGAATA TCATCAATAT TCAAGAAATC 
ATCCGTTCGG AGACTGTCGA TAGTTTGAAG GATATTTACA TAGTTCAATG CCTGATGGAG 
ACTGATTTGT ACAAATTGCT GAAAACTCAA AAGCTCTCAA ATGATCACGT TTGCTACTTT 
CTCTATCAAA TTCTCCGTGG TCTCAAATAC ATTCACTCTG CAAATGTGCT CCATCGTGAT 
TTGAAACCAT CGAATTTGTT GCTCAACACC ACATGTGATC TCAAAATTTG CGATTTTGGA 
TTGGCACGTG TCACCGACCC ACAAACCGAT CATACTGGGT TCTTGACCGA GTATGTGGCT 
ACTCGTTGGT ATAGAGCTCC AGAGATTATG CTCAATTCAA AGGGATACAC TAAATCGATT 
GATGTCTGGT CTGTCGGATG TATTCTCGCA GAAATGCTCA GTAATCGGCC ATTGTTCCCG 
GGAAAACATT ATTTGGATCA ACTCAATTTG ATTTTGGCAG TTGTTGGATC CCCGTCAAAT 
GCTGATTTAC AATGTATTAT TAATGATAAG GCTCGATCAT ATCTCATTTC ATTGCCACAC 
AAACCAAAAC AACCATGGGC TCGGCTCTAT CCAGGAGCTG ATCCAAGAGC TCTTGATTTA 
TTGGACAAAA TGCTCACTTT CAATCCACAT AATCGTATCG ACATCGAGCA AGCATTGGCT 
CACCCATACT TGGAGCAATA CTACGATCCA GGAGATGAGC CAGTTTGTGA GGAACCATTC 
ACTTTGGAAA TGGAATTCGA CGATTTACCG AAGGAGAAGC TGAAGGAGCT GATTTGGGAA 
GAAGCCGAGG CTCATCACAG ACGAATGGAG GCAGAAGCGG CTGCAAGGAA TAATGGAGGG 
CAGAATCCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022584.1
CDS	15..1349
Translation	MPTWIPNNLCAQPTTRNAKPPSNGHPQATQQQSAPGSLAYRNSSNIPNGATNHVRQQKWQYTRSGHRKMADGEAVISTVNNVEEVHGQLFEVAPRYVNLSYIGEGAYGMVASALDTITRDRVAIKKISPFEHQTFCQRTLREIKILNRFKHENIINIQEIIRSETVDSLKDIYIVQCLMETDLYKLLKTQKLSNDHVCYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVTDPQTDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDVWSVGCILAEMLSNRPLFPGKHYLDQLNLILAVVGSPSNADLQCIINDKARSYLISLPHKPKQPWARLYPGADPRALDLLDKMLTFNPHNRIDIEQALAHPYLEQYYDPGDEPVCEEPFTLEMEFDDLPKEKLKELIWEEAEAHHRRMEAEAAARNNGGQNPV

Target ORF information:

RefSeq Version	NM_001027413.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027413.3

ORF Insert Sequence:

ATGCCAACGT GGATACCTAA CAATTTGTGC GCACAGCCGA CAACGAGAAA TGCTAAACCA 
CCATCGAACG GGCATCCACA AGCGACACAA CAACAATCTG CTCCGGGATC ATTGGCATAC 
CGTAATTCGA GCAACATACC GAATGGAGCA ACCAACCACG TCAGACAACA GAAATGGCAG 
TACACTCGTT CAGGACATAG AAAGATGGCC GACGGAGAAG CGGTTATCTC GACGGTCAAC 
AATGTCGAGG AAGTTCATGG GCAACTTTTT GAGGTTGCTC CCCGTTATGT GAATCTTTCA 
TATATTGGAG AAGGTGCTTA CGGGATGGTC GCCTCTGCAC TTGACACAAT TACTCGTGAT 
CGCGTTGCTA TCAAAAAGAT TTCTCCATTC GAACATCAGA CATTCTGTCA ACGGACACTT 
CGGGAAATCA AAATTCTTAA TCGATTCAAG CATGAGAATA TCATCAATAT TCAAGAAATC 
ATCCGTTCGG AGACTGTCGA TAGTTTGAAG GATATTTACA TAGTTCAATG CCTGATGGAG 
ACTGATTTGT ACAAATTGCT GAAAACTCAA AAGCTCTCAA ATGATCACGT TTGCTACTTT 
CTCTATCAAA TTCTCCGTGG TCTCAAATAC ATTCACTCTG CAAATGTGCT CCATCGTGAT 
TTGAAACCAT CGAATTTGTT GCTCAACACC ACATGTGATC TCAAAATTTG CGATTTTGGA 
TTGGCACGTG TCACCGACCC ACAAACCGAT CATACTGGGT TCTTGACCGA GTATGTGGCT 
ACTCGTTGGT ATAGAGCTCC AGAGATTATG CTCAATTCAA AGGGATACAC TAAATCGATT 
GATGTCTGGT CTGTCGGATG TATTCTCGCA GAAATGCTCA GTAATCGGCC ATTGTTCCCG 
GGAAAACATT ATTTGGATCA ACTCAATTTG ATTTTGGCAG TTGTTGGATC CCCGTCAAAT 
GCTGATTTAC AATGTATTAT TAATGATAAG GCTCGATCAT ATCTCATTTC ATTGCCACAC 
AAACCAAAAC AACCATGGGC TCGGCTCTAT CCAGGAGCTG ATCCAAGAGC TCTTGATTTA 
TTGGACAAAA TGCTCACTTT CAATCCACAT AATCGTATCG ACATCGAGCA AGCATTGGCT 
CACCCATACT TGGAGCAATA CTACGATCCA GGAGATGAGC CAGTTTGTGA GGAACCATTC 
ACTTTGGAAA TGGAATTCGA CGATTTACCG AAGGAGAAGC TGAAGGAGCT GATTTGGGAA 
GAAGCCGAGG CTCATCACAG ACGAATGGAG GCAGAAGCGG CTGCAAGGAA TAATGGAGGG 
CAGAATCCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mpk-1 ( NM_001027413.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022584.1 Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

Price & Availability↑

CloneID	OCa340695
Clone ID Related Accession (Same CDS sequence)	NM_001027413.3 , NM_001027413.2
Accession Version	NM_001027413.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Mitogen-activated protein kinase mpk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 27, 2008 this sequence version replaced NM_001027413.1. COMPLETENESS: incomplete on both ends.

ATGCCAACGT GGATACCTAA CAATTTGTGC GCACAGCCGA CAACGAGAAA TGCTAAACCA 
CCATCGAACG GGCATCCACA AGCGACACAA CAACAATCTG CTCCGGGATC ATTGGCATAC 
CGTAATTCGA GCAACATACC GAATGGAGCA ACCAACCACG TCAGACAACA GAAATGGCAG 
TACACTCGTT CAGGACATAG AAAGATGGCC GACGGAGAAG CGGTTATCTC GACGGTCAAC 
AATGTCGAGG AAGTTCATGG GCAACTTTTT GAGGTTGCTC CCCGTTATGT GAATCTTTCA 
TATATTGGAG AAGGTGCTTA CGGGATGGTC GCCTCTGCAC TTGACACAAT TACTCGTGAT 
CGCGTTGCTA TCAAAAAGAT TTCTCCATTC GAACATCAGA CATTCTGTCA ACGGACACTT 
CGGGAAATCA AAATTCTTAA TCGATTCAAG CATGAGAATA TCATCAATAT TCAAGAAATC 
ATCCGTTCGG AGACTGTCGA TAGTTTGAAG GATATTTACA TAGTTCAATG CCTGATGGAG 
ACTGATTTGT ACAAATTGCT GAAAACTCAA AAGCTCTCAA ATGATCACGT TTGCTACTTT 
CTCTATCAAA TTCTCCGTGG TCTCAAATAC ATTCACTCTG CAAATGTGCT CCATCGTGAT 
TTGAAACCAT CGAATTTGTT GCTCAACACC ACATGTGATC TCAAAATTTG CGATTTTGGA 
TTGGCACGTG TCACCGACCC ACAAACCGAT CATACTGGGT TCTTGACCGA GTATGTGGCT 
ACTCGTTGGT ATAGAGCTCC AGAGATTATG CTCAATTCAA AGGGATACAC TAAATCGATT 
GATGTCTGGT CTGTCGGATG TATTCTCGCA GAAATGCTCA GTAATCGGCC ATTGTTCCCG 
GGAAAACATT ATTTGGATCA ACTCAATTTG ATTTTGGCAG TTGTTGGATC CCCGTCAAAT 
GCTGATTTAC AATGTATTAT TAATGATAAG GCTCGATCAT ATCTCATTTC ATTGCCACAC 
AAACCAAAAC AACCATGGGC TCGGCTCTAT CCAGGAGCTG ATCCAAGAGC TCTTGATTTA 
TTGGACAAAA TGCTCACTTT CAATCCACAT AATCGTATCG ACATCGAGCA AGCATTGGCT 
CACCCATACT TGGAGCAATA CTACGATCCA GGAGATGAGC CAGTTTGTGA GGAACCATTC 
ACTTTGGAAA TGGAATTCGA CGATTTACCG AAGGAGAAGC TGAAGGAGCT GATTTGGGAA 
GAAGCCGAGG CTCATCACAG ACGAATGGAG GCAGAAGCGG CTGCAAGGAA TAATGGAGGG 
CAGAATCCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022584.1
CDS	1..1335
Translation	MPTWIPNNLCAQPTTRNAKPPSNGHPQATQQQSAPGSLAYRNSSNIPNGATNHVRQQKWQYTRSGHRKMADGEAVISTVNNVEEVHGQLFEVAPRYVNLSYIGEGAYGMVASALDTITRDRVAIKKISPFEHQTFCQRTLREIKILNRFKHENIINIQEIIRSETVDSLKDIYIVQCLMETDLYKLLKTQKLSNDHVCYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVTDPQTDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDVWSVGCILAEMLSNRPLFPGKHYLDQLNLILAVVGSPSNADLQCIINDKARSYLISLPHKPKQPWARLYPGADPRALDLLDKMLTFNPHNRIDIEQALAHPYLEQYYDPGDEPVCEEPFTLEMEFDDLPKEKLKELIWEEAEAHHRRMEAEAAARNNGGQNPV

Target ORF information:

RefSeq Version	NM_001027413.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027413.2

ORF Insert Sequence:

ATGCCAACGT GGATACCTAA CAATTTGTGC GCACAGCCGA CAACGAGAAA TGCTAAACCA 
CCATCGAACG GGCATCCACA AGCGACACAA CAACAATCTG CTCCGGGATC ATTGGCATAC 
CGTAATTCGA GCAACATACC GAATGGAGCA ACCAACCACG TCAGACAACA GAAATGGCAG 
TACACTCGTT CAGGACATAG AAAGATGGCC GACGGAGAAG CGGTTATCTC GACGGTCAAC 
AATGTCGAGG AAGTTCATGG GCAACTTTTT GAGGTTGCTC CCCGTTATGT GAATCTTTCA 
TATATTGGAG AAGGTGCTTA CGGGATGGTC GCCTCTGCAC TTGACACAAT TACTCGTGAT 
CGCGTTGCTA TCAAAAAGAT TTCTCCATTC GAACATCAGA CATTCTGTCA ACGGACACTT 
CGGGAAATCA AAATTCTTAA TCGATTCAAG CATGAGAATA TCATCAATAT TCAAGAAATC 
ATCCGTTCGG AGACTGTCGA TAGTTTGAAG GATATTTACA TAGTTCAATG CCTGATGGAG 
ACTGATTTGT ACAAATTGCT GAAAACTCAA AAGCTCTCAA ATGATCACGT TTGCTACTTT 
CTCTATCAAA TTCTCCGTGG TCTCAAATAC ATTCACTCTG CAAATGTGCT CCATCGTGAT 
TTGAAACCAT CGAATTTGTT GCTCAACACC ACATGTGATC TCAAAATTTG CGATTTTGGA 
TTGGCACGTG TCACCGACCC ACAAACCGAT CATACTGGGT TCTTGACCGA GTATGTGGCT 
ACTCGTTGGT ATAGAGCTCC AGAGATTATG CTCAATTCAA AGGGATACAC TAAATCGATT 
GATGTCTGGT CTGTCGGATG TATTCTCGCA GAAATGCTCA GTAATCGGCC ATTGTTCCCG 
GGAAAACATT ATTTGGATCA ACTCAATTTG ATTTTGGCAG TTGTTGGATC CCCGTCAAAT 
GCTGATTTAC AATGTATTAT TAATGATAAG GCTCGATCAT ATCTCATTTC ATTGCCACAC 
AAACCAAAAC AACCATGGGC TCGGCTCTAT CCAGGAGCTG ATCCAAGAGC TCTTGATTTA 
TTGGACAAAA TGCTCACTTT CAATCCACAT AATCGTATCG ACATCGAGCA AGCATTGGCT 
CACCCATACT TGGAGCAATA CTACGATCCA GGAGATGAGC CAGTTTGTGA GGAACCATTC 
ACTTTGGAAA TGGAATTCGA CGATTTACCG AAGGAGAAGC TGAAGGAGCT GATTTGGGAA 
GAAGCCGAGG CTCATCACAG ACGAATGGAG GCAGAAGCGG CTGCAAGGAA TAATGGAGGG 
CAGAATCCTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mpk-1 ( NM_001027412.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022583.1 Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

Price & Availability↑

CloneID	OCa341997
Clone ID Related Accession (Same CDS sequence)	NM_001027412.3 , NM_001027412.4
Accession Version	NM_001027412.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Mitogen-activated protein kinase mpk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_001027412.2. COMPLETENESS: incomplete on both ends.

ATGGCCGACG GAGAAGCGGT TATCTCGACG GTCAACAATG TCGAGGAAGT TCATGGGCAA 
CTTTTTGAGG TTGCTCCCCG TTATGTGAAT CTTTCATATA TTGGAGAAGG TGCTTACGGG 
ATGGTCGCCT CTGCACTTGA CACAATTACT CGTGATCGCG TTGCTATCAA AAAGATTTCT 
CCATTCGAAC ATCAGACATT CTGTCAACGG ACACTTCGGG AAATCAAAAT TCTTAATCGA 
TTCAAGCATG AGAATATCAT CAATATTCAA GAAATCATCC GTTCGGAGAC TGTCGATAGT 
TTGAAGGATA TTTACATAGT TCAATGCCTG ATGGAGACTG ATTTGTACAA ATTGCTGAAA 
ACTCAAAAGC TCTCAAATGA TCACGTTTGC TACTTTCTCT ATCAAATTCT CCGTGGTCTC 
AAATACATTC ACTCTGCAAA TGTGCTCCAT CGTGATTTGA AACCATCGAA TTTGTTGCTC 
AACACCACAT GTGATCTCAA AATTTGCGAT TTTGGATTGG CACGTGTCAC CGACCCACAA 
ACCGATCATA CTGGGTTCTT GACCGAGTAT GTGGCTACTC GTTGGTATAG AGCTCCAGAG 
ATTATGCTCA ATTCAAAGGG ATACACTAAA TCGATTGATG TCTGGTCTGT CGGATGTATT 
CTCGCAGAAA TGCTCAGTAA TCGGCCATTG TTCCCGGGAA AACATTATTT GGATCAACTC 
AATTTGATTT TGGCAGTTGT TGGATCCCCG TCAAATGCTG ATTTACAATG TATTATTAAT 
GATAAGGCTC GATCATATCT CATTTCATTG CCACACAAAC CAAAACAACC ATGGGCTCGG 
CTCTATCCAG GAGCTGATCC AAGAGCTCTT GATTTATTGG ACAAAATGCT CACTTTCAAT 
CCACATAATC GTATCGACAT CGAGCAAGCA TTGGCTCACC CATACTTGGA GCAATACTAC 
GATCCAGGAG ATGAGCCAGT TTGTGAGGAA CCATTCACTT TGGAAATGGA ATTCGACGAT 
TTACCGAAGG AGAAGCTGAA GGAGCTGATT TGGGAAGAAG CCGAGGCTCA TCACAGACGA 
ATGGAGGCAG AAGCGGCTGC AAGGAATAAT GGAGGGCAGA ATCCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022583.1
CDS	1..1131
Translation	MADGEAVISTVNNVEEVHGQLFEVAPRYVNLSYIGEGAYGMVASALDTITRDRVAIKKISPFEHQTFCQRTLREIKILNRFKHENIINIQEIIRSETVDSLKDIYIVQCLMETDLYKLLKTQKLSNDHVCYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVTDPQTDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDVWSVGCILAEMLSNRPLFPGKHYLDQLNLILAVVGSPSNADLQCIINDKARSYLISLPHKPKQPWARLYPGADPRALDLLDKMLTFNPHNRIDIEQALAHPYLEQYYDPGDEPVCEEPFTLEMEFDDLPKEKLKELIWEEAEAHHRRMEAEAAARNNGGQNPV

Target ORF information:

RefSeq Version	NM_001027412.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027412.3

ORF Insert Sequence:

ATGGCCGACG GAGAAGCGGT TATCTCGACG GTCAACAATG TCGAGGAAGT TCATGGGCAA 
CTTTTTGAGG TTGCTCCCCG TTATGTGAAT CTTTCATATA TTGGAGAAGG TGCTTACGGG 
ATGGTCGCCT CTGCACTTGA CACAATTACT CGTGATCGCG TTGCTATCAA AAAGATTTCT 
CCATTCGAAC ATCAGACATT CTGTCAACGG ACACTTCGGG AAATCAAAAT TCTTAATCGA 
TTCAAGCATG AGAATATCAT CAATATTCAA GAAATCATCC GTTCGGAGAC TGTCGATAGT 
TTGAAGGATA TTTACATAGT TCAATGCCTG ATGGAGACTG ATTTGTACAA ATTGCTGAAA 
ACTCAAAAGC TCTCAAATGA TCACGTTTGC TACTTTCTCT ATCAAATTCT CCGTGGTCTC 
AAATACATTC ACTCTGCAAA TGTGCTCCAT CGTGATTTGA AACCATCGAA TTTGTTGCTC 
AACACCACAT GTGATCTCAA AATTTGCGAT TTTGGATTGG CACGTGTCAC CGACCCACAA 
ACCGATCATA CTGGGTTCTT GACCGAGTAT GTGGCTACTC GTTGGTATAG AGCTCCAGAG 
ATTATGCTCA ATTCAAAGGG ATACACTAAA TCGATTGATG TCTGGTCTGT CGGATGTATT 
CTCGCAGAAA TGCTCAGTAA TCGGCCATTG TTCCCGGGAA AACATTATTT GGATCAACTC 
AATTTGATTT TGGCAGTTGT TGGATCCCCG TCAAATGCTG ATTTACAATG TATTATTAAT 
GATAAGGCTC GATCATATCT CATTTCATTG CCACACAAAC CAAAACAACC ATGGGCTCGG 
CTCTATCCAG GAGCTGATCC AAGAGCTCTT GATTTATTGG ACAAAATGCT CACTTTCAAT 
CCACATAATC GTATCGACAT CGAGCAAGCA TTGGCTCACC CATACTTGGA GCAATACTAC 
GATCCAGGAG ATGAGCCAGT TTGTGAGGAA CCATTCACTT TGGAAATGGA ATTCGACGAT 
TTACCGAAGG AGAAGCTGAA GGAGCTGATT TGGGAAGAAG CCGAGGCTCA TCACAGACGA 
ATGGAGGCAG AAGCGGCTGC AAGGAATAAT GGAGGGCAGA ATCCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mpk-1 ( NM_001027412.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022583.1 Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

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CloneID	OCa341997
Clone ID Related Accession (Same CDS sequence)	NM_001027412.3 , NM_001027412.4
Accession Version	NM_001027412.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Mitogen-activated protein kinase mpk-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001027412.3. COMPLETENESS: incomplete on the 5' end.

ATGGCCGACG GAGAAGCGGT TATCTCGACG GTCAACAATG TCGAGGAAGT TCATGGGCAA 
CTTTTTGAGG TTGCTCCCCG TTATGTGAAT CTTTCATATA TTGGAGAAGG TGCTTACGGG 
ATGGTCGCCT CTGCACTTGA CACAATTACT CGTGATCGCG TTGCTATCAA AAAGATTTCT 
CCATTCGAAC ATCAGACATT CTGTCAACGG ACACTTCGGG AAATCAAAAT TCTTAATCGA 
TTCAAGCATG AGAATATCAT CAATATTCAA GAAATCATCC GTTCGGAGAC TGTCGATAGT 
TTGAAGGATA TTTACATAGT TCAATGCCTG ATGGAGACTG ATTTGTACAA ATTGCTGAAA 
ACTCAAAAGC TCTCAAATGA TCACGTTTGC TACTTTCTCT ATCAAATTCT CCGTGGTCTC 
AAATACATTC ACTCTGCAAA TGTGCTCCAT CGTGATTTGA AACCATCGAA TTTGTTGCTC 
AACACCACAT GTGATCTCAA AATTTGCGAT TTTGGATTGG CACGTGTCAC CGACCCACAA 
ACCGATCATA CTGGGTTCTT GACCGAGTAT GTGGCTACTC GTTGGTATAG AGCTCCAGAG 
ATTATGCTCA ATTCAAAGGG ATACACTAAA TCGATTGATG TCTGGTCTGT CGGATGTATT 
CTCGCAGAAA TGCTCAGTAA TCGGCCATTG TTCCCGGGAA AACATTATTT GGATCAACTC 
AATTTGATTT TGGCAGTTGT TGGATCCCCG TCAAATGCTG ATTTACAATG TATTATTAAT 
GATAAGGCTC GATCATATCT CATTTCATTG CCACACAAAC CAAAACAACC ATGGGCTCGG 
CTCTATCCAG GAGCTGATCC AAGAGCTCTT GATTTATTGG ACAAAATGCT CACTTTCAAT 
CCACATAATC GTATCGACAT CGAGCAAGCA TTGGCTCACC CATACTTGGA GCAATACTAC 
GATCCAGGAG ATGAGCCAGT TTGTGAGGAA CCATTCACTT TGGAAATGGA ATTCGACGAT 
TTACCGAAGG AGAAGCTGAA GGAGCTGATT TGGGAAGAAG CCGAGGCTCA TCACAGACGA 
ATGGAGGCAG AAGCGGCTGC AAGGAATAAT GGAGGGCAGA ATCCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022583.1
CDS	1..1131
Translation	MADGEAVISTVNNVEEVHGQLFEVAPRYVNLSYIGEGAYGMVASALDTITRDRVAIKKISPFEHQTFCQRTLREIKILNRFKHENIINIQEIIRSETVDSLKDIYIVQCLMETDLYKLLKTQKLSNDHVCYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVTDPQTDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDVWSVGCILAEMLSNRPLFPGKHYLDQLNLILAVVGSPSNADLQCIINDKARSYLISLPHKPKQPWARLYPGADPRALDLLDKMLTFNPHNRIDIEQALAHPYLEQYYDPGDEPVCEEPFTLEMEFDDLPKEKLKELIWEEAEAHHRRMEAEAAARNNGGQNPV

Target ORF information:

RefSeq Version	NM_001027412.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mitogen-activated protein kinase mpk-1 (mpk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027412.4

ORF Insert Sequence:

ATGGCCGACG GAGAAGCGGT TATCTCGACG GTCAACAATG TCGAGGAAGT TCATGGGCAA 
CTTTTTGAGG TTGCTCCCCG TTATGTGAAT CTTTCATATA TTGGAGAAGG TGCTTACGGG 
ATGGTCGCCT CTGCACTTGA CACAATTACT CGTGATCGCG TTGCTATCAA AAAGATTTCT 
CCATTCGAAC ATCAGACATT CTGTCAACGG ACACTTCGGG AAATCAAAAT TCTTAATCGA 
TTCAAGCATG AGAATATCAT CAATATTCAA GAAATCATCC GTTCGGAGAC TGTCGATAGT 
TTGAAGGATA TTTACATAGT TCAATGCCTG ATGGAGACTG ATTTGTACAA ATTGCTGAAA 
ACTCAAAAGC TCTCAAATGA TCACGTTTGC TACTTTCTCT ATCAAATTCT CCGTGGTCTC 
AAATACATTC ACTCTGCAAA TGTGCTCCAT CGTGATTTGA AACCATCGAA TTTGTTGCTC 
AACACCACAT GTGATCTCAA AATTTGCGAT TTTGGATTGG CACGTGTCAC CGACCCACAA 
ACCGATCATA CTGGGTTCTT GACCGAGTAT GTGGCTACTC GTTGGTATAG AGCTCCAGAG 
ATTATGCTCA ATTCAAAGGG ATACACTAAA TCGATTGATG TCTGGTCTGT CGGATGTATT 
CTCGCAGAAA TGCTCAGTAA TCGGCCATTG TTCCCGGGAA AACATTATTT GGATCAACTC 
AATTTGATTT TGGCAGTTGT TGGATCCCCG TCAAATGCTG ATTTACAATG TATTATTAAT 
GATAAGGCTC GATCATATCT CATTTCATTG CCACACAAAC CAAAACAACC ATGGGCTCGG 
CTCTATCCAG GAGCTGATCC AAGAGCTCTT GATTTATTGG ACAAAATGCT CACTTTCAAT 
CCACATAATC GTATCGACAT CGAGCAAGCA TTGGCTCACC CATACTTGGA GCAATACTAC 
GATCCAGGAG ATGAGCCAGT TTGTGAGGAA CCATTCACTT TGGAAATGGA ATTCGACGAT 
TTACCGAAGG AGAAGCTGAA GGAGCTGATT TGGGAAGAAG CCGAGGCTCA TCACAGACGA 
ATGGAGGCAG AAGCGGCTGC AAGGAATAAT GGAGGGCAGA ATCCTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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mpk-1 cDNA ORF clone, Caenorhabditis elegans

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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