unc-45 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-45
Entrez Gene ID	175206
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064804.5	NP_497205.1	hypothetical protein
NM_064804.6	NP_497205.1	TPR_REGION domain-containing protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Unc45b	NP_001100498.1
Danio rerio (zebrafish)	unc45b	NP_705959.1
Drosophila melanogaster (fruit fly)	unc-45	NP_524796.1
Pan troglodytes (chimpanzee)	UNC45B	XP_001174363.2
Mus musculus (house mouse)	Unc45b	NP_848795.3
Gallus gallus (chicken)	UNC45B	XP_004946569.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003727	XP_310258.5
Canis lupus familiaris (dog)	UNC45B	XP_005624856.1
Bos taurus (cattle)	UNC45B	XP_002695676.1
Xenopus tropicalis (tropical clawed frog)	unc45b	NP_001172057.1
Homo sapiens (human)	UNC45B	NP_775259.1
	UNC45B	XP_001113905.2
Caenorhabditis elegans (roundworm)	unc-45	NP_497205.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Biochemical and structural analyses with in vivo studies of the C. elegans UNC-45 protein reveal a chaperone chain in which UNC-45 tandem modules assemble a multisite docking platform that enforces collaboration with Hsp70 and Hsp90 in a precisely defined pattern to assemble myosin filaments.
Title: The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.

UNC-45 is not required to fold or maintain the actin binding domain but is still required for myosin function.
Title: UNC-45 is required for NMY-2 contractile function in early embryonic polarity establishment and germline cellularization in C. elegans.

Study reports that transgenic worms overexpressing UNC-45 display defects in myosin assembly, with decreased myosin content and a mild paralysis phenotype.
Title: The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans.

UNC-45 interacts with more than one type of myosin and functions in the embryo to regulate cytoplasmic myosin assembly and/or stability during cytokinesis
Title: Maternal UNC-45 is involved in cytokinesis and colocalizes with non-muscle myosin in the early Caenorhabditis elegans embryo.

The following unc-45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa352152	NM_064804.6 Latest version!	Caenorhabditis elegans TPR_REGION domain-containing protein (unc-45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa352152	NM_064804.5	Caenorhabditis elegans Uncharacterized protein (unc-45), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-45 ( NM_064804.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497205.1 Caenorhabditis elegans Uncharacterized protein (unc-45), partial mRNA.

Price & Availability↑

CloneID	OCa352152
Clone ID Related Accession (Same CDS sequence)	NM_064804.5 , NM_064804.6
Accession Version	NM_064804.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_064804.4. COMPLETENESS: incomplete on both ends.

ATGGTTGCTC GAGTACAGAC TGCGGAGGAG ATCCGCGACG AGGGAAATGC TGCAGTTAAG 
GATCAGGATT ATATAAAAGC TGATGAATTA TACACTGAAG CTCTCCAACT CACCACTGAC 
GAAGACAAGG CTCTTCGGCC GGTTTTGTAC AGAAATCGAG CAATGGCTCG GCTGAAAAGA 
GACGATTTTG AAGGAGCACA ATCGGATTGC ACGAAAGCTC TGGAATTCGA CGGGGCCGAT 
GTAAAAGCTC TCTTCAGACG ATCGCTGGCT CGTGAACAGC TTGGAAATGT TGGGCCAGCT 
TTTCAAGACG CAAAAGAGGC TCTGAGGCTC TCGCCAAATG ATAAAGGAAT TGTTGAAGTT 
CTTCAGCGTC TCGTAAAAGC CAACAATGAT AAGATTAAGC AGACCACTTC ACTGGCTAAT 
AAGGTAACTG ACATGGAAAA GCTAGCGTTC CGTGGTGAGG CGAAGGACAC CGAACAGAAA 
ATGACTGCGT TGAACAACTT GTTGGTTTTG TGCAGAGAAT CTGAATCCGG AGCAACAGGA 
GTTTGGAATC AGGGCGCACT TGTTCCATTC GTCCTGAACC TAATCAACGA CGCATCGGAA 
AACGAGGAGG TTACCGTAAC CGCAATCCGA ATTCTCGACG AGACCATCAA AAATAGCGTC 
AGATGCATGA AATTCCTAGC TATGCACGAC CCAGATGGCC CAAAATCCGT CCGTTTTGTG 
TGCCGGCTAA TGTGCAAAAA ATCCACAAAA GACTTTGTGG ATGCCACTGG AATTCTGGTG 
CAACGTGTCT TCAATGCAAT GGCTAAAATG GATCGGCAAA AGGAGATGAA GCCGGATCCA 
GAGGTGGCAG AAGCCAATAA AATCTGGATA ATTCGTGTGC TCTTGGAGCT TCAAGAGATG 
CTTCAGGACC CGAAAGTTGG TGCAGTTCAG CGTGAGACGT GCATCGATTT ATTCCTGAAA 
AATCTGATGC ACATGGACGG TGGAATTCCA CGTGGATGGT CGTGGAAGTT TGTGGAAGAA 
CGTGGATTGC TCGCGTTGCT TGACGTGGCA AGTCAGATAC CCGAACTGTG TGAATATCCG 
GTTTCTGCGG AGACTCGGCA ACACGTGGCA ATTTGTTTGC AGAGATTAGA GGAGGATATG 
GTTTTTGATA CGAAACGGAC TATTTTTAAG GAGAAAGTCG ATATGTTCTT TAATGCCCTA 
ATCTCCCGTT GCACGAATGA CGATGAAGGT CACAAATACC GTATCAAACT ATCATGCTTC 
TTGATTACAA TGCTTCAAGG ACCAGTTGAT ATTGGAATAA ATCTTATCAC AAATGATCAA 
TTAACTCCGA TTATGCTCGA AATGGCCGCT TCACAGGATC ATTTAATGCA AGGAATCGCT 
GCTGAGCTCA TTGTTGCAAC TGTTAGCAAA CACGAAAGAG CTATTAATAT GTTGAAAGTC 
GGAATTCCGG TGCTCCGTGC GTTGTACGAC TCTGAGGATC CTACTGTCAA GGTCCGAGCT 
CTAGTTGGTC TTTGCAAGAT CGGAGCCGCC GGAGGAGACG ACATTTCGAA AGCAACGATG 
AAAGAAGAAG CAGTGATCAG TTTGGCGAAG ACTTGCAAGA AATTCCTTCT GGAAACCGAA 
AAATATTCAG TTGATATTAG AAGATACGCG TGTGAAGGGC TCTCCTATTT GTCTTTGGAT 
GCTGATGTTA AGGAATGGAT TGTTGATGAT TCTCTGTTGC TCAAAGCTTT AGTACTTTTA 
GCAAAAAAGG CAGGAGCTCT ATGTGTTTAT ACACTAGCAA CTATCTATGC AAATCTCTCG 
AATGCATTTG AAAAGCCAAA AGTCGACGAG GAAATGGTAA AACTCGCGCA ATTCGCGAAG 
CATCACGTCC CAGAAACTCA TCCCAAGGAT ACGGAGGAAT ATGTTGAAAA ACGAGTGAGA 
GCTCTCGTTG AGGAGGGAGC CGTTCCGGCT TGTGTGGCGG TTAGTAAGAC GGAAAGTAAG 
AATGCGTTGG AGCTGATCGC GAGAAGTCTT TTGGCGTTTG CTGAGTACGA GGATCTTCGA 
GGACGGATTA TTGCTGAAGG TGGAACGGTT TTGTGCTTAA GACTTACGAA GGAAGCTTCT 
GGAGAGGGAA AGATCAAGGC GGGACATGCG ATTGCTAAGC TTGGGGCTAA AGCGGATCCG 
ATGATTTCTT TCCCTGGACA ACGAGCATAC GAAGTGGTAA AACCGCTTTG CGACCTGCTC 
CATCCGGACG TTGAAGGAAA AGCCAACTAC GATTCTCTTC TGACACTCAC CAACTTGGCA 
AGCGTCAGTG ATTCGATTCG TGGACGAATT TTGAAAGAGA AGGCAATTCC AAAGATTGAG 
GAATTCTGGT TTATGACGGA TCACGAGCAT TTGAGAGCCG CCGCCGCTGA GCTTCTTCTC 
AATTTGCTCT TTTTCGAGAA GTTTTATGAG GAAACTGTTG CGCCCGGAAC CGATCGTCTG 
AAACTCTGGG TTCTCTACTC GGCAGAAGTT GAAGAGGAAC GTCTATCACG TGCCTCGGCG 
GCAGGTTTCG CCATTCTTAC AGAGGACGAG AACGCTTGTG CTAGGATTAT GGACGAGATT 
AAATCATGGC CTGAGGTGTT CAAAGACATT GCAATGCACG AGGATGCGGA AACCCAACGT 
CGTGGCCTTA TGGGAATTGC GAATATAATG CACTCGAGCA ACAAACTGTG CTCCGAAATT 
GTATCGTCCG AAGTGTTCCG TGTCCTGGTC GCCGTCACAA AGCTCGGCAC TATCAATCAG 
GAACGGGCAG GCTCGACGGA ACAGGCGAAA CGTGGATTGG AGGCCGCCGA GAAGTTTGGA 
CTGATTAAGG CGACGGATCG AGAGATTTAT GAACGCGAAA ATCAAATGAG CACCATTCAG 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497205.1
CDS	1..2886
Translation	MVARVQTAEEIRDEGNAAVKDQDYIKADELYTEALQLTTDEDKALRPVLYRNRAMARLKRDDFEGAQSDCTKALEFDGADVKALFRRSLAREQLGNVGPAFQDAKEALRLSPNDKGIVEVLQRLVKANNDKIKQTTSLANKVTDMEKLAFRGEAKDTEQKMTALNNLLVLCRESESGATGVWNQGALVPFVLNLINDASENEEVTVTAIRILDETIKNSVRCMKFLAMHDPDGPKSVRFVCRLMCKKSTKDFVDATGILVQRVFNAMAKMDRQKEMKPDPEVAEANKIWIIRVLLELQEMLQDPKVGAVQRETCIDLFLKNLMHMDGGIPRGWSWKFVEERGLLALLDVASQIPELCEYPVSAETRQHVAICLQRLEEDMVFDTKRTIFKEKVDMFFNALISRCTNDDEGHKYRIKLSCFLITMLQGPVDIGINLITNDQLTPIMLEMAASQDHLMQGIAAELIVATVSKHERAINMLKVGIPVLRALYDSEDPTVKVRALVGLCKIGAAGGDDISKATMKEEAVISLAKTCKKFLLETEKYSVDIRRYACEGLSYLSLDADVKEWIVDDSLLLKALVLLAKKAGALCVYTLATIYANLSNAFEKPKVDEEMVKLAQFAKHHVPETHPKDTEEYVEKRVRALVEEGAVPACVAVSKTESKNALELIARSLLAFAEYEDLRGRIIAEGGTVLCLRLTKEASGEGKIKAGHAIAKLGAKADPMISFPGQRAYEVVKPLCDLLHPDVEGKANYDSLLTLTNLASVSDSIRGRILKEKAIPKIEEFWFMTDHEHLRAAAAELLLNLLFFEKFYEETVAPGTDRLKLWVLYSAEVEEERLSRASAAGFAILTEDENACARIMDEIKSWPEVFKDIAMHEDAETQRRGLMGIANIMHSSNKLCSEIVSSEVFRVLVAVTKLGTINQERAGSTEQAKRGLEAAEKFGLIKATDREIYERENQMSTIQE

Target ORF information:

RefSeq Version	NM_064804.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-45), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064804.5

ORF Insert Sequence:

ATGGTTGCTC GAGTACAGAC TGCGGAGGAG ATCCGCGACG AGGGAAATGC TGCAGTTAAG 
GATCAGGATT ATATAAAAGC TGATGAATTA TACACTGAAG CTCTCCAACT CACCACTGAC 
GAAGACAAGG CTCTTCGGCC GGTTTTGTAC AGAAATCGAG CAATGGCTCG GCTGAAAAGA 
GACGATTTTG AAGGAGCACA ATCGGATTGC ACGAAAGCTC TGGAATTCGA CGGGGCCGAT 
GTAAAAGCTC TCTTCAGACG ATCGCTGGCT CGTGAACAGC TTGGAAATGT TGGGCCAGCT 
TTTCAAGACG CAAAAGAGGC TCTGAGGCTC TCGCCAAATG ATAAAGGAAT TGTTGAAGTT 
CTTCAGCGTC TCGTAAAAGC CAACAATGAT AAGATTAAGC AGACCACTTC ACTGGCTAAT 
AAGGTAACTG ACATGGAAAA GCTAGCGTTC CGTGGTGAGG CGAAGGACAC CGAACAGAAA 
ATGACTGCGT TGAACAACTT GTTGGTTTTG TGCAGAGAAT CTGAATCCGG AGCAACAGGA 
GTTTGGAATC AGGGCGCACT TGTTCCATTC GTCCTGAACC TAATCAACGA CGCATCGGAA 
AACGAGGAGG TTACCGTAAC CGCAATCCGA ATTCTCGACG AGACCATCAA AAATAGCGTC 
AGATGCATGA AATTCCTAGC TATGCACGAC CCAGATGGCC CAAAATCCGT CCGTTTTGTG 
TGCCGGCTAA TGTGCAAAAA ATCCACAAAA GACTTTGTGG ATGCCACTGG AATTCTGGTG 
CAACGTGTCT TCAATGCAAT GGCTAAAATG GATCGGCAAA AGGAGATGAA GCCGGATCCA 
GAGGTGGCAG AAGCCAATAA AATCTGGATA ATTCGTGTGC TCTTGGAGCT TCAAGAGATG 
CTTCAGGACC CGAAAGTTGG TGCAGTTCAG CGTGAGACGT GCATCGATTT ATTCCTGAAA 
AATCTGATGC ACATGGACGG TGGAATTCCA CGTGGATGGT CGTGGAAGTT TGTGGAAGAA 
CGTGGATTGC TCGCGTTGCT TGACGTGGCA AGTCAGATAC CCGAACTGTG TGAATATCCG 
GTTTCTGCGG AGACTCGGCA ACACGTGGCA ATTTGTTTGC AGAGATTAGA GGAGGATATG 
GTTTTTGATA CGAAACGGAC TATTTTTAAG GAGAAAGTCG ATATGTTCTT TAATGCCCTA 
ATCTCCCGTT GCACGAATGA CGATGAAGGT CACAAATACC GTATCAAACT ATCATGCTTC 
TTGATTACAA TGCTTCAAGG ACCAGTTGAT ATTGGAATAA ATCTTATCAC AAATGATCAA 
TTAACTCCGA TTATGCTCGA AATGGCCGCT TCACAGGATC ATTTAATGCA AGGAATCGCT 
GCTGAGCTCA TTGTTGCAAC TGTTAGCAAA CACGAAAGAG CTATTAATAT GTTGAAAGTC 
GGAATTCCGG TGCTCCGTGC GTTGTACGAC TCTGAGGATC CTACTGTCAA GGTCCGAGCT 
CTAGTTGGTC TTTGCAAGAT CGGAGCCGCC GGAGGAGACG ACATTTCGAA AGCAACGATG 
AAAGAAGAAG CAGTGATCAG TTTGGCGAAG ACTTGCAAGA AATTCCTTCT GGAAACCGAA 
AAATATTCAG TTGATATTAG AAGATACGCG TGTGAAGGGC TCTCCTATTT GTCTTTGGAT 
GCTGATGTTA AGGAATGGAT TGTTGATGAT TCTCTGTTGC TCAAAGCTTT AGTACTTTTA 
GCAAAAAAGG CAGGAGCTCT ATGTGTTTAT ACACTAGCAA CTATCTATGC AAATCTCTCG 
AATGCATTTG AAAAGCCAAA AGTCGACGAG GAAATGGTAA AACTCGCGCA ATTCGCGAAG 
CATCACGTCC CAGAAACTCA TCCCAAGGAT ACGGAGGAAT ATGTTGAAAA ACGAGTGAGA 
GCTCTCGTTG AGGAGGGAGC CGTTCCGGCT TGTGTGGCGG TTAGTAAGAC GGAAAGTAAG 
AATGCGTTGG AGCTGATCGC GAGAAGTCTT TTGGCGTTTG CTGAGTACGA GGATCTTCGA 
GGACGGATTA TTGCTGAAGG TGGAACGGTT TTGTGCTTAA GACTTACGAA GGAAGCTTCT 
GGAGAGGGAA AGATCAAGGC GGGACATGCG ATTGCTAAGC TTGGGGCTAA AGCGGATCCG 
ATGATTTCTT TCCCTGGACA ACGAGCATAC GAAGTGGTAA AACCGCTTTG CGACCTGCTC 
CATCCGGACG TTGAAGGAAA AGCCAACTAC GATTCTCTTC TGACACTCAC CAACTTGGCA 
AGCGTCAGTG ATTCGATTCG TGGACGAATT TTGAAAGAGA AGGCAATTCC AAAGATTGAG 
GAATTCTGGT TTATGACGGA TCACGAGCAT TTGAGAGCCG CCGCCGCTGA GCTTCTTCTC 
AATTTGCTCT TTTTCGAGAA GTTTTATGAG GAAACTGTTG CGCCCGGAAC CGATCGTCTG 
AAACTCTGGG TTCTCTACTC GGCAGAAGTT GAAGAGGAAC GTCTATCACG TGCCTCGGCG 
GCAGGTTTCG CCATTCTTAC AGAGGACGAG AACGCTTGTG CTAGGATTAT GGACGAGATT 
AAATCATGGC CTGAGGTGTT CAAAGACATT GCAATGCACG AGGATGCGGA AACCCAACGT 
CGTGGCCTTA TGGGAATTGC GAATATAATG CACTCGAGCA ACAAACTGTG CTCCGAAATT 
GTATCGTCCG AAGTGTTCCG TGTCCTGGTC GCCGTCACAA AGCTCGGCAC TATCAATCAG 
GAACGGGCAG GCTCGACGGA ACAGGCGAAA CGTGGATTGG AGGCCGCCGA GAAGTTTGGA 
CTGATTAAGG CGACGGATCG AGAGATTTAT GAACGCGAAA ATCAAATGAG CACCATTCAG 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-45 ( NM_064804.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497205.1 Caenorhabditis elegans TPR_REGION domain-containing protein (unc-45), mRNA.

Price & Availability↑

CloneID	OCa352152
Clone ID Related Accession (Same CDS sequence)	NM_064804.5 , NM_064804.6
Accession Version	NM_064804.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	TPR_REGION domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_064804.5.

ATGGTTGCTC GAGTACAGAC TGCGGAGGAG ATCCGCGACG AGGGAAATGC TGCAGTTAAG 
GATCAGGATT ATATAAAAGC TGATGAATTA TACACTGAAG CTCTCCAACT CACCACTGAC 
GAAGACAAGG CTCTTCGGCC GGTTTTGTAC AGAAATCGAG CAATGGCTCG GCTGAAAAGA 
GACGATTTTG AAGGAGCACA ATCGGATTGC ACGAAAGCTC TGGAATTCGA CGGGGCCGAT 
GTAAAAGCTC TCTTCAGACG ATCGCTGGCT CGTGAACAGC TTGGAAATGT TGGGCCAGCT 
TTTCAAGACG CAAAAGAGGC TCTGAGGCTC TCGCCAAATG ATAAAGGAAT TGTTGAAGTT 
CTTCAGCGTC TCGTAAAAGC CAACAATGAT AAGATTAAGC AGACCACTTC ACTGGCTAAT 
AAGGTAACTG ACATGGAAAA GCTAGCGTTC CGTGGTGAGG CGAAGGACAC CGAACAGAAA 
ATGACTGCGT TGAACAACTT GTTGGTTTTG TGCAGAGAAT CTGAATCCGG AGCAACAGGA 
GTTTGGAATC AGGGCGCACT TGTTCCATTC GTCCTGAACC TAATCAACGA CGCATCGGAA 
AACGAGGAGG TTACCGTAAC CGCAATCCGA ATTCTCGACG AGACCATCAA AAATAGCGTC 
AGATGCATGA AATTCCTAGC TATGCACGAC CCAGATGGCC CAAAATCCGT CCGTTTTGTG 
TGCCGGCTAA TGTGCAAAAA ATCCACAAAA GACTTTGTGG ATGCCACTGG AATTCTGGTG 
CAACGTGTCT TCAATGCAAT GGCTAAAATG GATCGGCAAA AGGAGATGAA GCCGGATCCA 
GAGGTGGCAG AAGCCAATAA AATCTGGATA ATTCGTGTGC TCTTGGAGCT TCAAGAGATG 
CTTCAGGACC CGAAAGTTGG TGCAGTTCAG CGTGAGACGT GCATCGATTT ATTCCTGAAA 
AATCTGATGC ACATGGACGG TGGAATTCCA CGTGGATGGT CGTGGAAGTT TGTGGAAGAA 
CGTGGATTGC TCGCGTTGCT TGACGTGGCA AGTCAGATAC CCGAACTGTG TGAATATCCG 
GTTTCTGCGG AGACTCGGCA ACACGTGGCA ATTTGTTTGC AGAGATTAGA GGAGGATATG 
GTTTTTGATA CGAAACGGAC TATTTTTAAG GAGAAAGTCG ATATGTTCTT TAATGCCCTA 
ATCTCCCGTT GCACGAATGA CGATGAAGGT CACAAATACC GTATCAAACT ATCATGCTTC 
TTGATTACAA TGCTTCAAGG ACCAGTTGAT ATTGGAATAA ATCTTATCAC AAATGATCAA 
TTAACTCCGA TTATGCTCGA AATGGCCGCT TCACAGGATC ATTTAATGCA AGGAATCGCT 
GCTGAGCTCA TTGTTGCAAC TGTTAGCAAA CACGAAAGAG CTATTAATAT GTTGAAAGTC 
GGAATTCCGG TGCTCCGTGC GTTGTACGAC TCTGAGGATC CTACTGTCAA GGTCCGAGCT 
CTAGTTGGTC TTTGCAAGAT CGGAGCCGCC GGAGGAGACG ACATTTCGAA AGCAACGATG 
AAAGAAGAAG CAGTGATCAG TTTGGCGAAG ACTTGCAAGA AATTCCTTCT GGAAACCGAA 
AAATATTCAG TTGATATTAG AAGATACGCG TGTGAAGGGC TCTCCTATTT GTCTTTGGAT 
GCTGATGTTA AGGAATGGAT TGTTGATGAT TCTCTGTTGC TCAAAGCTTT AGTACTTTTA 
GCAAAAAAGG CAGGAGCTCT ATGTGTTTAT ACACTAGCAA CTATCTATGC AAATCTCTCG 
AATGCATTTG AAAAGCCAAA AGTCGACGAG GAAATGGTAA AACTCGCGCA ATTCGCGAAG 
CATCACGTCC CAGAAACTCA TCCCAAGGAT ACGGAGGAAT ATGTTGAAAA ACGAGTGAGA 
GCTCTCGTTG AGGAGGGAGC CGTTCCGGCT TGTGTGGCGG TTAGTAAGAC GGAAAGTAAG 
AATGCGTTGG AGCTGATCGC GAGAAGTCTT TTGGCGTTTG CTGAGTACGA GGATCTTCGA 
GGACGGATTA TTGCTGAAGG TGGAACGGTT TTGTGCTTAA GACTTACGAA GGAAGCTTCT 
GGAGAGGGAA AGATCAAGGC GGGACATGCG ATTGCTAAGC TTGGGGCTAA AGCGGATCCG 
ATGATTTCTT TCCCTGGACA ACGAGCATAC GAAGTGGTAA AACCGCTTTG CGACCTGCTC 
CATCCGGACG TTGAAGGAAA AGCCAACTAC GATTCTCTTC TGACACTCAC CAACTTGGCA 
AGCGTCAGTG ATTCGATTCG TGGACGAATT TTGAAAGAGA AGGCAATTCC AAAGATTGAG 
GAATTCTGGT TTATGACGGA TCACGAGCAT TTGAGAGCCG CCGCCGCTGA GCTTCTTCTC 
AATTTGCTCT TTTTCGAGAA GTTTTATGAG GAAACTGTTG CGCCCGGAAC CGATCGTCTG 
AAACTCTGGG TTCTCTACTC GGCAGAAGTT GAAGAGGAAC GTCTATCACG TGCCTCGGCG 
GCAGGTTTCG CCATTCTTAC AGAGGACGAG AACGCTTGTG CTAGGATTAT GGACGAGATT 
AAATCATGGC CTGAGGTGTT CAAAGACATT GCAATGCACG AGGATGCGGA AACCCAACGT 
CGTGGCCTTA TGGGAATTGC GAATATAATG CACTCGAGCA ACAAACTGTG CTCCGAAATT 
GTATCGTCCG AAGTGTTCCG TGTCCTGGTC GCCGTCACAA AGCTCGGCAC TATCAATCAG 
GAACGGGCAG GCTCGACGGA ACAGGCGAAA CGTGGATTGG AGGCCGCCGA GAAGTTTGGA 
CTGATTAAGG CGACGGATCG AGAGATTTAT GAACGCGAAA ATCAAATGAG CACCATTCAG 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497205.1
CDS	3..2888
Translation	MVARVQTAEEIRDEGNAAVKDQDYIKADELYTEALQLTTDEDKALRPVLYRNRAMARLKRDDFEGAQSDCTKALEFDGADVKALFRRSLAREQLGNVGPAFQDAKEALRLSPNDKGIVEVLQRLVKANNDKIKQTTSLANKVTDMEKLAFRGEAKDTEQKMTALNNLLVLCRESESGATGVWNQGALVPFVLNLINDASENEEVTVTAIRILDETIKNSVRCMKFLAMHDPDGPKSVRFVCRLMCKKSTKDFVDATGILVQRVFNAMAKMDRQKEMKPDPEVAEANKIWIIRVLLELQEMLQDPKVGAVQRETCIDLFLKNLMHMDGGIPRGWSWKFVEERGLLALLDVASQIPELCEYPVSAETRQHVAICLQRLEEDMVFDTKRTIFKEKVDMFFNALISRCTNDDEGHKYRIKLSCFLITMLQGPVDIGINLITNDQLTPIMLEMAASQDHLMQGIAAELIVATVSKHERAINMLKVGIPVLRALYDSEDPTVKVRALVGLCKIGAAGGDDISKATMKEEAVISLAKTCKKFLLETEKYSVDIRRYACEGLSYLSLDADVKEWIVDDSLLLKALVLLAKKAGALCVYTLATIYANLSNAFEKPKVDEEMVKLAQFAKHHVPETHPKDTEEYVEKRVRALVEEGAVPACVAVSKTESKNALELIARSLLAFAEYEDLRGRIIAEGGTVLCLRLTKEASGEGKIKAGHAIAKLGAKADPMISFPGQRAYEVVKPLCDLLHPDVEGKANYDSLLTLTNLASVSDSIRGRILKEKAIPKIEEFWFMTDHEHLRAAAAELLLNLLFFEKFYEETVAPGTDRLKLWVLYSAEVEEERLSRASAAGFAILTEDENACARIMDEIKSWPEVFKDIAMHEDAETQRRGLMGIANIMHSSNKLCSEIVSSEVFRVLVAVTKLGTINQERAGSTEQAKRGLEAAEKFGLIKATDREIYERENQMSTIQE

Target ORF information:

RefSeq Version	NM_064804.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TPR_REGION domain-containing protein (unc-45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064804.6

ORF Insert Sequence:

ATGGTTGCTC GAGTACAGAC TGCGGAGGAG ATCCGCGACG AGGGAAATGC TGCAGTTAAG 
GATCAGGATT ATATAAAAGC TGATGAATTA TACACTGAAG CTCTCCAACT CACCACTGAC 
GAAGACAAGG CTCTTCGGCC GGTTTTGTAC AGAAATCGAG CAATGGCTCG GCTGAAAAGA 
GACGATTTTG AAGGAGCACA ATCGGATTGC ACGAAAGCTC TGGAATTCGA CGGGGCCGAT 
GTAAAAGCTC TCTTCAGACG ATCGCTGGCT CGTGAACAGC TTGGAAATGT TGGGCCAGCT 
TTTCAAGACG CAAAAGAGGC TCTGAGGCTC TCGCCAAATG ATAAAGGAAT TGTTGAAGTT 
CTTCAGCGTC TCGTAAAAGC CAACAATGAT AAGATTAAGC AGACCACTTC ACTGGCTAAT 
AAGGTAACTG ACATGGAAAA GCTAGCGTTC CGTGGTGAGG CGAAGGACAC CGAACAGAAA 
ATGACTGCGT TGAACAACTT GTTGGTTTTG TGCAGAGAAT CTGAATCCGG AGCAACAGGA 
GTTTGGAATC AGGGCGCACT TGTTCCATTC GTCCTGAACC TAATCAACGA CGCATCGGAA 
AACGAGGAGG TTACCGTAAC CGCAATCCGA ATTCTCGACG AGACCATCAA AAATAGCGTC 
AGATGCATGA AATTCCTAGC TATGCACGAC CCAGATGGCC CAAAATCCGT CCGTTTTGTG 
TGCCGGCTAA TGTGCAAAAA ATCCACAAAA GACTTTGTGG ATGCCACTGG AATTCTGGTG 
CAACGTGTCT TCAATGCAAT GGCTAAAATG GATCGGCAAA AGGAGATGAA GCCGGATCCA 
GAGGTGGCAG AAGCCAATAA AATCTGGATA ATTCGTGTGC TCTTGGAGCT TCAAGAGATG 
CTTCAGGACC CGAAAGTTGG TGCAGTTCAG CGTGAGACGT GCATCGATTT ATTCCTGAAA 
AATCTGATGC ACATGGACGG TGGAATTCCA CGTGGATGGT CGTGGAAGTT TGTGGAAGAA 
CGTGGATTGC TCGCGTTGCT TGACGTGGCA AGTCAGATAC CCGAACTGTG TGAATATCCG 
GTTTCTGCGG AGACTCGGCA ACACGTGGCA ATTTGTTTGC AGAGATTAGA GGAGGATATG 
GTTTTTGATA CGAAACGGAC TATTTTTAAG GAGAAAGTCG ATATGTTCTT TAATGCCCTA 
ATCTCCCGTT GCACGAATGA CGATGAAGGT CACAAATACC GTATCAAACT ATCATGCTTC 
TTGATTACAA TGCTTCAAGG ACCAGTTGAT ATTGGAATAA ATCTTATCAC AAATGATCAA 
TTAACTCCGA TTATGCTCGA AATGGCCGCT TCACAGGATC ATTTAATGCA AGGAATCGCT 
GCTGAGCTCA TTGTTGCAAC TGTTAGCAAA CACGAAAGAG CTATTAATAT GTTGAAAGTC 
GGAATTCCGG TGCTCCGTGC GTTGTACGAC TCTGAGGATC CTACTGTCAA GGTCCGAGCT 
CTAGTTGGTC TTTGCAAGAT CGGAGCCGCC GGAGGAGACG ACATTTCGAA AGCAACGATG 
AAAGAAGAAG CAGTGATCAG TTTGGCGAAG ACTTGCAAGA AATTCCTTCT GGAAACCGAA 
AAATATTCAG TTGATATTAG AAGATACGCG TGTGAAGGGC TCTCCTATTT GTCTTTGGAT 
GCTGATGTTA AGGAATGGAT TGTTGATGAT TCTCTGTTGC TCAAAGCTTT AGTACTTTTA 
GCAAAAAAGG CAGGAGCTCT ATGTGTTTAT ACACTAGCAA CTATCTATGC AAATCTCTCG 
AATGCATTTG AAAAGCCAAA AGTCGACGAG GAAATGGTAA AACTCGCGCA ATTCGCGAAG 
CATCACGTCC CAGAAACTCA TCCCAAGGAT ACGGAGGAAT ATGTTGAAAA ACGAGTGAGA 
GCTCTCGTTG AGGAGGGAGC CGTTCCGGCT TGTGTGGCGG TTAGTAAGAC GGAAAGTAAG 
AATGCGTTGG AGCTGATCGC GAGAAGTCTT TTGGCGTTTG CTGAGTACGA GGATCTTCGA 
GGACGGATTA TTGCTGAAGG TGGAACGGTT TTGTGCTTAA GACTTACGAA GGAAGCTTCT 
GGAGAGGGAA AGATCAAGGC GGGACATGCG ATTGCTAAGC TTGGGGCTAA AGCGGATCCG 
ATGATTTCTT TCCCTGGACA ACGAGCATAC GAAGTGGTAA AACCGCTTTG CGACCTGCTC 
CATCCGGACG TTGAAGGAAA AGCCAACTAC GATTCTCTTC TGACACTCAC CAACTTGGCA 
AGCGTCAGTG ATTCGATTCG TGGACGAATT TTGAAAGAGA AGGCAATTCC AAAGATTGAG 
GAATTCTGGT TTATGACGGA TCACGAGCAT TTGAGAGCCG CCGCCGCTGA GCTTCTTCTC 
AATTTGCTCT TTTTCGAGAA GTTTTATGAG GAAACTGTTG CGCCCGGAAC CGATCGTCTG 
AAACTCTGGG TTCTCTACTC GGCAGAAGTT GAAGAGGAAC GTCTATCACG TGCCTCGGCG 
GCAGGTTTCG CCATTCTTAC AGAGGACGAG AACGCTTGTG CTAGGATTAT GGACGAGATT 
AAATCATGGC CTGAGGTGTT CAAAGACATT GCAATGCACG AGGATGCGGA AACCCAACGT 
CGTGGCCTTA TGGGAATTGC GAATATAATG CACTCGAGCA ACAAACTGTG CTCCGAAATT 
GTATCGTCCG AAGTGTTCCG TGTCCTGGTC GCCGTCACAA AGCTCGGCAC TATCAATCAG 
GAACGGGCAG GCTCGACGGA ACAGGCGAAA CGTGGATTGG AGGCCGCCGA GAAGTTTGGA 
CTGATTAAGG CGACGGATCG AGAGATTTAT GAACGCGAAA ATCAAATGAG CACCATTCAG 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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unc-45 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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