rpoa-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rpoa-1
Entrez Gene ID	175015
Full Name	DNA-directed RNA polymerase subunit
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001027279.3	NP_001022450.1	DNA-directed RNA polymerase subunit
NM_001027280.4	NP_001022451.1	RNA_pol_Rpb1_5 domain-containing protein
NM_001027280.3	NP_001022451.1	DNA-directed RNA polymerase subunit
NM_001027279.4	NP_001022450.1	DNA-directed RNA polymerase subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rpoa-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpoa-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338213	NM_001027280.4 Latest version!	Caenorhabditis elegans RNA_pol_Rpb1_5 domain-containing protein (rpoa-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OCa337770	NM_001027279.4 Latest version!	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa338213	NM_001027280.3	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OCa337770	NM_001027279.3	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpoa-1 ( NM_001027280.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022451.1 Caenorhabditis elegans RNA_pol_Rpb1_5 domain-containing protein (rpoa-1), mRNA.

Price & Availability↑

CloneID	OCa338213
Clone ID Related Accession (Same CDS sequence)	NM_001027280.4 , NM_001027280.3
Accession Version	NM_001027280.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	RNA_pol_Rpb1_5 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027280.3.

ATGGAAATGA ACAACGTGTT CGCCGTCTAC GGTATCGAGG TCTCGAAGCG ACATCTCTCT 
CTAACTGCCG ACTACATGAC ATTCACCGGA CAAATCCAAC CATTCAACCG CGGAGCCATG 
TCGTCGAGCT CATCACCCCT CCAAAAAATG ACGTTCGAAA CGACGATGGC CTTCTTGCGA 
GAGGCGCTCT TGCATGGCGA AGAAGACAAT GTGAACTCTC CGTCGGCTCG TCTCGTAATG 
GGAGCCCTTC CGCGTGGCGG TACCGGATCG TTCGACTTGC TGCTCGACAC GAAAATGCAA 
TCCGAACGGG AGGAGCACGA GGCGGCGAGG GCTCGAAAGC GGAACGCCAA GCAGAAGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022451.1
CDS	276..638
Translation	MEMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGALPRGGTGSFDLLLDTKMQSEREEHEAARARKRNAKQKF

Target ORF information:

RefSeq Version	NM_001027280.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans RNA_pol_Rpb1_5 domain-containing protein (rpoa-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027280.4

ORF Insert Sequence:

ATGGAAATGA ACAACGTGTT CGCCGTCTAC GGTATCGAGG TCTCGAAGCG ACATCTCTCT 
CTAACTGCCG ACTACATGAC ATTCACCGGA CAAATCCAAC CATTCAACCG CGGAGCCATG 
TCGTCGAGCT CATCACCCCT CCAAAAAATG ACGTTCGAAA CGACGATGGC CTTCTTGCGA 
GAGGCGCTCT TGCATGGCGA AGAAGACAAT GTGAACTCTC CGTCGGCTCG TCTCGTAATG 
GGAGCCCTTC CGCGTGGCGG TACCGGATCG TTCGACTTGC TGCTCGACAC GAAAATGCAA 
TCCGAACGGG AGGAGCACGA GGCGGCGAGG GCTCGAAAGC GGAACGCCAA GCAGAAGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpoa-1 ( NM_001027280.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022451.1 Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.

Price & Availability↑

CloneID	OCa338213
Clone ID Related Accession (Same CDS sequence)	NM_001027280.4 , NM_001027280.3
Accession Version	NM_001027280.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027280.2. COMPLETENESS: incomplete on both ends.

ATGGAAATGA ACAACGTGTT CGCCGTCTAC GGTATCGAGG TCTCGAAGCG ACATCTCTCT 
CTAACTGCCG ACTACATGAC ATTCACCGGA CAAATCCAAC CATTCAACCG CGGAGCCATG 
TCGTCGAGCT CATCACCCCT CCAAAAAATG ACGTTCGAAA CGACGATGGC CTTCTTGCGA 
GAGGCGCTCT TGCATGGCGA AGAAGACAAT GTGAACTCTC CGTCGGCTCG TCTCGTAATG 
GGAGCCCTTC CGCGTGGCGG TACCGGATCG TTCGACTTGC TGCTCGACAC GAAAATGCAA 
TCCGAACGGG AGGAGCACGA GGCGGCGAGG GCTCGAAAGC GGAACGCCAA GCAGAAGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022451.1
CDS	1..363
Translation	MEMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGALPRGGTGSFDLLLDTKMQSEREEHEAARARKRNAKQKF

Target ORF information:

RefSeq Version	NM_001027280.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027280.3

ORF Insert Sequence:

ATGGAAATGA ACAACGTGTT CGCCGTCTAC GGTATCGAGG TCTCGAAGCG ACATCTCTCT 
CTAACTGCCG ACTACATGAC ATTCACCGGA CAAATCCAAC CATTCAACCG CGGAGCCATG 
TCGTCGAGCT CATCACCCCT CCAAAAAATG ACGTTCGAAA CGACGATGGC CTTCTTGCGA 
GAGGCGCTCT TGCATGGCGA AGAAGACAAT GTGAACTCTC CGTCGGCTCG TCTCGTAATG 
GGAGCCCTTC CGCGTGGCGG TACCGGATCG TTCGACTTGC TGCTCGACAC GAAAATGCAA 
TCCGAACGGG AGGAGCACGA GGCGGCGAGG GCTCGAAAGC GGAACGCCAA GCAGAAGTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpoa-1 ( NM_001027279.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022450.1 Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.

Price & Availability↑

CloneID	OCa337770
Clone ID Related Accession (Same CDS sequence)	NM_001027279.3 , NM_001027279.4
Accession Version	NM_001027279.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027279.2. COMPLETENESS: incomplete on both ends.

ATGGATTTCT TCGTCAGAAA TGGCGAGGAG CCGTATATGC AATTCTCGAA TTTCAAGCTG 
CGGAGCTATT TTCCGCATGA AATCGACAAG CTCAGTGTGC TCAAAATCAC GCAGACAAAG 
ACTTTCGATG AGGTCGGTCA CCCGATTGCC GGCGGTCTTT ACGACCCGAT TTTGGGCCCT 
GACAACACTT TCGACATGTG CATGACGTGT AATCAATACG AACGTCATTG TCCAGGACAT 
ATGGGACATA TTCAGCTGGC GGTTCCGGTT TTCAACCCGC TTCTCTTCCA GTTCACCTAT 
AATCTTCTCA AAGGCTCCTG CGTCCACTGC CATCGATTAA CCTGCAAAGG AGACGGAGTG 
AATGCCCGAA TGCTGCTCGC CCAGCTCAGA TGCTTCGAGC TCGGCGTTGA ACACGTGGCG 
TTCGATCTGG AATCAATTCT GCGGGATAAA ATCGCAAATG CTGATCTTTT CGGCGATGAC 
GATTCGAAAA CCTTCAACGA CGTGGATGCT TGTATCGCTC AGCTCGCGAA CAAGCCGCTT 
TCGGAGCTCA CCGCGTTCAA ATCGATGCCA ACCAAGAATA GTGTTCAGCT GAAAAAAGAT 
ATTATCACCG AATTCCTCAG AAGTCACCTG TTCAAACGTC TCCAAAAATG TCCGCTCTGC 
AAGAATCGCA ACGGAGTCCT GCGAAACGAC GGAGCTCGCT CGATTCTCAT CGATTTCACC 
TCCGGGGCTC GCGGCGGCGG AAAATCCAAA AAAGCCATTA TCATCATCGG AGATAATGGA 
TATAACGAGG AATCTTCGAC AGATGAGGAT GAGGAGAAGA AGGGCGGCGG CGGCGCAGAG 
GTCACCGGAG ATGTTGTGGA TTTGGATGAA GGAAGCCTGG AGATGCAGAT GAAGAATGTG 
AGAACTGGAG AATGCGACAA ACTAGCGTGG AGAGGTGCTG AAGTCCGCGA GCACTTCAGG 
ATGCTGTTCA AAAACGACGG AAAGCTGCTG CTCAAGCTGT TCCCGATGCT TGTCGACGAG 
CTCAACGGAG AGGACATGAT CTGCCCGCTA GACGGTCTCT TCTTGGAGCG AATCCTGGTT 
CCACCGAAGA AATTCCGCCC GATCCGAATG TTCAAGGGCG CTCAATACGA GGATCCACAG 
ACTCTGAACC TTCGTAAAGT TCTCGAAGCG ACCGAGACAA TCTCGGCGAT CTCTCTAATC 
ATGAAAGGCG ACACAAGTGC TCAGTTGAAG GAGCTCATCG CGAATCGGGT CCGCGGGAAG 
ACGATCAACG CGCAGATGCA CGACGCCTAC CTGCAGCTTC AGCTTCGCGC CAACGCGATC 
TTCGATCAAG ATCTCAACAA AGGCGACAGG GATAGCATCG CCGGTATCAA GCAGATCTTG 
GAGAAGAAGC AGGGATTGTT CAGAATGCAT ATGATGGGAA AACGAGTCAA CTTTGCTTGT 
CGATCAGTCA TCACTCCGGA TCCCTACCTG GATATTGATG AGATCGGAAT TCCGGATATT 
TTCGCGAAAA AGCTCACTTT CACAGAGCCA GTGAATGCTT TTAACGTGAA CGAGATGAAG 
GGGCTTCTCC GCCAAGGCCC CCACCAGCAC CCGGGAGCCA ATTTCTTCGT GGAGCCTTCC 
GGCAAGAAGA CGATGCTCGG TGATAAGCCT GAAGAGAAGA AGCGTCGGAT GCAAATGGCC 
AAAACCCTCA ACGCGGCGAC CACAGAAAAC CTCCGCCAAA CGCCAAAAGT GTTGAGACAC 
ATGAAAAACG GCGATATGAT CATGATGAAT CGTCAGCCAT CGCTTCACAA GCCGTCGATC 
CTCGGCCACC GAGCCCGTGT GCTCACCGGA CAGCGGGCTC TTCGGATGAA CTACGCGCCT 
TGCAAGGCCT ACAATGCGGA TTTCGATGGA GACGAGATGA ACGGACACTT GGTGCAATCA 
CATATCGCGC AGACGGAAGT TCGCGAGATT GCGAACGTCG GAAGTAACTT TTTGGTGCCG 
AAGGATGCGA CACCGCTGTT GGGATTGATT CAGGATCACG TGGTGTCGGG TGTCTTGTTG 
AGTGTTCGGG ATCGATTCCT GAATAAGGAG GATTTTATGC ACCTTGTGCT CGCCTCATTC 
GCCCAGTACT CGAAGAGGAT CGAGATTCCA CCACCGACGA TTCTCTATCC GAAGCGGCTT 
TGGACTGGAA AGCAGGTCAT CACCACAATC GTCAAAAATT GTATTCCAGA CGGAAAACCG 
CTCATCAATC TCGACGGAAA AGCCAAGACA CCGCTCAGCT GCTGGATTGT GCCGGGATTC 
GATGCTCCGC AATTCGACAT GAGTGAATCT CATGTGGTGT TCAGGCAAGG AGAGCTGCTC 
GTCGGAGTTC TCGATAAGGC TCACTTTGGA GCAACGCAAT TTGGATTGGC TCATTGTGCA 
TTTGAGCTTT ATGGACATCG GTGTGGAGTG CAGTTGTTAT CGTGTTTTTC GCGGCTTTTT 
ACGACCTATT TGCAGTTCCA CGGCTTCACC CTCGGAGTCG CCGACATTCT CGTCGTCAAG 
GATGCCGACG GAAAACGCAA GGAAGCCGTC ATGGAATCCA GAACCATTGG AAATCAAGTC 
GTCAAGACAG CGTTCGGCCT TCCAGACACT GCGACGCCGG CAGAGATCAA GCGAACGCTC 
GCAGCCACCT ATTGTAATCC ACGTGGACAA GGGACTGACG TGAAAATGTT GGATTTCGGT 
ATGAAGCAAG GAATCGCCAA ATACAACGAC GCCATCACAA AATCCTGTGT GCCGACTGGT 
CTTCTCCGGC TTTTCCCACA AAATGCTCTG CAACTGATGA TTCAATCCGG AGCCAAAGGA 
TCCGCAGTCA ATGCTATTCA GATTTCAGGC TGTCTAGGCC AAATTGAGTT GGAAGGCAAG 
CGTATGGCTG TGACGATCGC CGGACGGACT CTCCCATCGT TCCGATGCTT TGATCCATCC 
CCGCGGGCAG GTGGATACAT TGATCAGCGA TTTTTGACCG GAATGAATCC ACAGGAGCTG 
TTCTTTCACA CAATGGCCGG TCGAGAAGGT CTTATTGATA CGGCTGTAAA GACTTCCCGG 
TCGGGATACC TCCAACGTTG TATCATCAAG CATTTGGAGG GAATCCGTGT GCACTACGAC 
TCGACCGTCA GGGATCATGA TGGATCGGTG ATTCAGTTTC GGTATGGAGA GGATGGAATG 
GACACGACGA AGGCGACTTT TTTGAATAAA AAGACTATGC CGTTCCTGGA AGATAATCTG 
GAAGCGGTGA CACTTGCGTC GAAGCCTGAA GGAGTTACAG ATGCTGATTT TGGAATTAAG 
GAGACCGAGA AGCGGTATAA AAAGATTGTT AAGTGGAAGA AAAAGGCCGA GAAATCTGGG 
AAATCGGGCA CAAAAAAGTC CTATTTCTCA GCATTCACAA ACTTCTCCGC GGAGCACATC 
GGCATGGAGA AGAAGCGGAT CCTTGCGATG TGGTTTGAGC TTTCCCTCGA AGAACGCGAG 
CAGTACGCCC GTGGAATTCC GAAAAAGTGT CCGGAAGCGG TGGATGAGAG GTTCAACCCG 
ACGTGCAAGC TTGGAGCCCT ACCGGAGAAG ATGTTGGATG AGATTGAGGG GTTCTGTACG 
CGGCGGGTGA AGAAGGTGGA TGATGAGGAG CCACCGAAGG AGGTGTTGAA GAGGACGCTG 
TATTGGAAAG GAATGAGATC CCTCGCCGAT CCAGGAGAGA ACGTCGGACT CCTCGCTGCT 
CAATCAATCG GAGAACCATC AACTCAAATG ACACTGAACA CTTTCCATTT TGCCGGTCGT 
GGAGAAATGA ACGTTACTCT AGGAATTCCG CGTCTTCGAG AAATTTTGAT GACTGCGTCG 
AAGAGCATCG CAACGCCAAG TGCATCGATT GCCGTCATCG CTGGAACATC AAGGGATCGT 
ATCGATTCGA TTAAGCGGGA GTTGGATCGA GTCTATTTGA AGCAATTGTT GAAGAATTTC 
TCGTTGGAAG AGAAGATTAC GTTGACACAA AATCAATCCT GCCGCCGCTA CCACCTCCGC 
ATCGACATTC TGGCCGCCGA GAAGCGTGAA CTCGGAGCCC GACACCTGAA ACGTTCGCAG 
ATTATGGAAG AAATCGAAAA GCGATTCATT CTCCGTGTGG CTCAGGCGAT TAAAAAGAAA 
TATCACGAGA TTACCGACTA TCAGCAAATG TCTCATCGTA CTATGCGTCA GGGAAATATG 
GCAGCGGGAA TTGAAACTGG CGGGACAAAG AATCGCGGAC TTCAGGGACC CGACAATGGA 
GATTCGTCTG ACGAGGAAGC CGACGGAGGA CGTGAAGCTG ATGCAGCTGA AGCTAGGCTT 
CATCGCAGAC ATCGAGATGA GGGGGCGGAT TATGAGGGAG AAGATGAGGA GAGAGTCGAG 
GTTCGAGAAG AAGAGGAGCC GATGGATTCG GATTCGGAAG ATGTGAAGAA GGAGGGATTG 
GATGGAGAGG ATCAGACGAC GGAGCCACTA CTGGTCAACT CGTCGAGAAT TCAATCTGTT 
CAGCGGCTTT CCGAGAATAT TTCATCGTAT ACGTATGATG TCAAGTCGAA TAAGTGGTGT 
GAAGTGGTTT TCGAGCTTCC ACTGAGGAAT AAGACCAAGA TGGATGTTTC TTCGATTGTT 
GAAAAAGAAG TCGAACTATT CATCGTCCAT CAAACTCCGG GAATCGAGCG ATGCGTCGAG 
ACGACTGAGC AGAAAAACGG AAAAGAAATG ACAATTCTGC AGACGCAAGG AGTCAATTTG 
GCTGCATTCT TCAAGCACGC AGACGTGCTC GACGTCAATT CGGTCTATTC GAACGATCTG 
AACTTGATTC TCGAGAATTA TGGAGTTGAA GCTTGCTCTA AGGCTATCAC TACTGAAATG 
AACAACGTGT TCGCCGTCTA CGGTATCGAG GTCTCGAAGC GACATCTCTC TCTAACTGCC 
GACTACATGA CATTCACCGG ACAAATCCAA CCATTCAACC GCGGAGCCAT GTCGTCGAGC 
TCATCACCCC TCCAAAAAAT GACGTTCGAA ACGACGATGG CCTTCTTGCG AGAGGCGCTC 
TTGCATGGCG AAGAAGACAA TGTGAACTCT CCGTCGGCTC GTCTCGTAAT GGGAGCCCTT 
CCGCGTGGCG GTACCGGATC GTTCGACTTG CTGCTCGACA CGAAAATGCA ATCCGAACGG 
GAGGAGCACG AGGCGGCGAG GGCTCGAAAG CGGAACGCCA AGCAGAAGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022450.1
CDS	1..5214
Translation	MDFFVRNGEEPYMQFSNFKLRSYFPHEIDKLSVLKITQTKTFDEVGHPIAGGLYDPILGPDNTFDMCMTCNQYERHCPGHMGHIQLAVPVFNPLLFQFTYNLLKGSCVHCHRLTCKGDGVNARMLLAQLRCFELGVEHVAFDLESILRDKIANADLFGDDDSKTFNDVDACIAQLANKPLSELTAFKSMPTKNSVQLKKDIITEFLRSHLFKRLQKCPLCKNRNGVLRNDGARSILIDFTSGARGGGKSKKAIIIIGDNGYNEESSTDEDEEKKGGGGAEVTGDVVDLDEGSLEMQMKNVRTGECDKLAWRGAEVREHFRMLFKNDGKLLLKLFPMLVDELNGEDMICPLDGLFLERILVPPKKFRPIRMFKGAQYEDPQTLNLRKVLEATETISAISLIMKGDTSAQLKELIANRVRGKTINAQMHDAYLQLQLRANAIFDQDLNKGDRDSIAGIKQILEKKQGLFRMHMMGKRVNFACRSVITPDPYLDIDEIGIPDIFAKKLTFTEPVNAFNVNEMKGLLRQGPHQHPGANFFVEPSGKKTMLGDKPEEKKRRMQMAKTLNAATTENLRQTPKVLRHMKNGDMIMMNRQPSLHKPSILGHRARVLTGQRALRMNYAPCKAYNADFDGDEMNGHLVQSHIAQTEVREIANVGSNFLVPKDATPLLGLIQDHVVSGVLLSVRDRFLNKEDFMHLVLASFAQYSKRIEIPPPTILYPKRLWTGKQVITTIVKNCIPDGKPLINLDGKAKTPLSCWIVPGFDAPQFDMSESHVVFRQGELLVGVLDKAHFGATQFGLAHCAFELYGHRCGVQLLSCFSRLFTTYLQFHGFTLGVADILVVKDADGKRKEAVMESRTIGNQVVKTAFGLPDTATPAEIKRTLAATYCNPRGQGTDVKMLDFGMKQGIAKYNDAITKSCVPTGLLRLFPQNALQLMIQSGAKGSAVNAIQISGCLGQIELEGKRMAVTIAGRTLPSFRCFDPSPRAGGYIDQRFLTGMNPQELFFHTMAGREGLIDTAVKTSRSGYLQRCIIKHLEGIRVHYDSTVRDHDGSVIQFRYGEDGMDTTKATFLNKKTMPFLEDNLEAVTLASKPEGVTDADFGIKETEKRYKKIVKWKKKAEKSGKSGTKKSYFSAFTNFSAEHIGMEKKRILAMWFELSLEEREQYARGIPKKCPEAVDERFNPTCKLGALPEKMLDEIEGFCTRRVKKVDDEEPPKEVLKRTLYWKGMRSLADPGENVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMTASKSIATPSASIAVIAGTSRDRIDSIKRELDRVYLKQLLKNFSLEEKITLTQNQSCRRYHLRIDILAAEKRELGARHLKRSQIMEEIEKRFILRVAQAIKKKYHEITDYQQMSHRTMRQGNMAAGIETGGTKNRGLQGPDNGDSSDEEADGGREADAAEARLHRRHRDEGADYEGEDEERVEVREEEEPMDSDSEDVKKEGLDGEDQTTEPLLVNSSRIQSVQRLSENISSYTYDVKSNKWCEVVFELPLRNKTKMDVSSIVEKEVELFIVHQTPGIERCVETTEQKNGKEMTILQTQGVNLAAFFKHADVLDVNSVYSNDLNLILENYGVEACSKAITTEMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGALPRGGTGSFDLLLDTKMQSEREEHEAARARKRNAKQKF

Target ORF information:

RefSeq Version	NM_001027279.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027279.3

ORF Insert Sequence:

ATGGATTTCT TCGTCAGAAA TGGCGAGGAG CCGTATATGC AATTCTCGAA TTTCAAGCTG 
CGGAGCTATT TTCCGCATGA AATCGACAAG CTCAGTGTGC TCAAAATCAC GCAGACAAAG 
ACTTTCGATG AGGTCGGTCA CCCGATTGCC GGCGGTCTTT ACGACCCGAT TTTGGGCCCT 
GACAACACTT TCGACATGTG CATGACGTGT AATCAATACG AACGTCATTG TCCAGGACAT 
ATGGGACATA TTCAGCTGGC GGTTCCGGTT TTCAACCCGC TTCTCTTCCA GTTCACCTAT 
AATCTTCTCA AAGGCTCCTG CGTCCACTGC CATCGATTAA CCTGCAAAGG AGACGGAGTG 
AATGCCCGAA TGCTGCTCGC CCAGCTCAGA TGCTTCGAGC TCGGCGTTGA ACACGTGGCG 
TTCGATCTGG AATCAATTCT GCGGGATAAA ATCGCAAATG CTGATCTTTT CGGCGATGAC 
GATTCGAAAA CCTTCAACGA CGTGGATGCT TGTATCGCTC AGCTCGCGAA CAAGCCGCTT 
TCGGAGCTCA CCGCGTTCAA ATCGATGCCA ACCAAGAATA GTGTTCAGCT GAAAAAAGAT 
ATTATCACCG AATTCCTCAG AAGTCACCTG TTCAAACGTC TCCAAAAATG TCCGCTCTGC 
AAGAATCGCA ACGGAGTCCT GCGAAACGAC GGAGCTCGCT CGATTCTCAT CGATTTCACC 
TCCGGGGCTC GCGGCGGCGG AAAATCCAAA AAAGCCATTA TCATCATCGG AGATAATGGA 
TATAACGAGG AATCTTCGAC AGATGAGGAT GAGGAGAAGA AGGGCGGCGG CGGCGCAGAG 
GTCACCGGAG ATGTTGTGGA TTTGGATGAA GGAAGCCTGG AGATGCAGAT GAAGAATGTG 
AGAACTGGAG AATGCGACAA ACTAGCGTGG AGAGGTGCTG AAGTCCGCGA GCACTTCAGG 
ATGCTGTTCA AAAACGACGG AAAGCTGCTG CTCAAGCTGT TCCCGATGCT TGTCGACGAG 
CTCAACGGAG AGGACATGAT CTGCCCGCTA GACGGTCTCT TCTTGGAGCG AATCCTGGTT 
CCACCGAAGA AATTCCGCCC GATCCGAATG TTCAAGGGCG CTCAATACGA GGATCCACAG 
ACTCTGAACC TTCGTAAAGT TCTCGAAGCG ACCGAGACAA TCTCGGCGAT CTCTCTAATC 
ATGAAAGGCG ACACAAGTGC TCAGTTGAAG GAGCTCATCG CGAATCGGGT CCGCGGGAAG 
ACGATCAACG CGCAGATGCA CGACGCCTAC CTGCAGCTTC AGCTTCGCGC CAACGCGATC 
TTCGATCAAG ATCTCAACAA AGGCGACAGG GATAGCATCG CCGGTATCAA GCAGATCTTG 
GAGAAGAAGC AGGGATTGTT CAGAATGCAT ATGATGGGAA AACGAGTCAA CTTTGCTTGT 
CGATCAGTCA TCACTCCGGA TCCCTACCTG GATATTGATG AGATCGGAAT TCCGGATATT 
TTCGCGAAAA AGCTCACTTT CACAGAGCCA GTGAATGCTT TTAACGTGAA CGAGATGAAG 
GGGCTTCTCC GCCAAGGCCC CCACCAGCAC CCGGGAGCCA ATTTCTTCGT GGAGCCTTCC 
GGCAAGAAGA CGATGCTCGG TGATAAGCCT GAAGAGAAGA AGCGTCGGAT GCAAATGGCC 
AAAACCCTCA ACGCGGCGAC CACAGAAAAC CTCCGCCAAA CGCCAAAAGT GTTGAGACAC 
ATGAAAAACG GCGATATGAT CATGATGAAT CGTCAGCCAT CGCTTCACAA GCCGTCGATC 
CTCGGCCACC GAGCCCGTGT GCTCACCGGA CAGCGGGCTC TTCGGATGAA CTACGCGCCT 
TGCAAGGCCT ACAATGCGGA TTTCGATGGA GACGAGATGA ACGGACACTT GGTGCAATCA 
CATATCGCGC AGACGGAAGT TCGCGAGATT GCGAACGTCG GAAGTAACTT TTTGGTGCCG 
AAGGATGCGA CACCGCTGTT GGGATTGATT CAGGATCACG TGGTGTCGGG TGTCTTGTTG 
AGTGTTCGGG ATCGATTCCT GAATAAGGAG GATTTTATGC ACCTTGTGCT CGCCTCATTC 
GCCCAGTACT CGAAGAGGAT CGAGATTCCA CCACCGACGA TTCTCTATCC GAAGCGGCTT 
TGGACTGGAA AGCAGGTCAT CACCACAATC GTCAAAAATT GTATTCCAGA CGGAAAACCG 
CTCATCAATC TCGACGGAAA AGCCAAGACA CCGCTCAGCT GCTGGATTGT GCCGGGATTC 
GATGCTCCGC AATTCGACAT GAGTGAATCT CATGTGGTGT TCAGGCAAGG AGAGCTGCTC 
GTCGGAGTTC TCGATAAGGC TCACTTTGGA GCAACGCAAT TTGGATTGGC TCATTGTGCA 
TTTGAGCTTT ATGGACATCG GTGTGGAGTG CAGTTGTTAT CGTGTTTTTC GCGGCTTTTT 
ACGACCTATT TGCAGTTCCA CGGCTTCACC CTCGGAGTCG CCGACATTCT CGTCGTCAAG 
GATGCCGACG GAAAACGCAA GGAAGCCGTC ATGGAATCCA GAACCATTGG AAATCAAGTC 
GTCAAGACAG CGTTCGGCCT TCCAGACACT GCGACGCCGG CAGAGATCAA GCGAACGCTC 
GCAGCCACCT ATTGTAATCC ACGTGGACAA GGGACTGACG TGAAAATGTT GGATTTCGGT 
ATGAAGCAAG GAATCGCCAA ATACAACGAC GCCATCACAA AATCCTGTGT GCCGACTGGT 
CTTCTCCGGC TTTTCCCACA AAATGCTCTG CAACTGATGA TTCAATCCGG AGCCAAAGGA 
TCCGCAGTCA ATGCTATTCA GATTTCAGGC TGTCTAGGCC AAATTGAGTT GGAAGGCAAG 
CGTATGGCTG TGACGATCGC CGGACGGACT CTCCCATCGT TCCGATGCTT TGATCCATCC 
CCGCGGGCAG GTGGATACAT TGATCAGCGA TTTTTGACCG GAATGAATCC ACAGGAGCTG 
TTCTTTCACA CAATGGCCGG TCGAGAAGGT CTTATTGATA CGGCTGTAAA GACTTCCCGG 
TCGGGATACC TCCAACGTTG TATCATCAAG CATTTGGAGG GAATCCGTGT GCACTACGAC 
TCGACCGTCA GGGATCATGA TGGATCGGTG ATTCAGTTTC GGTATGGAGA GGATGGAATG 
GACACGACGA AGGCGACTTT TTTGAATAAA AAGACTATGC CGTTCCTGGA AGATAATCTG 
GAAGCGGTGA CACTTGCGTC GAAGCCTGAA GGAGTTACAG ATGCTGATTT TGGAATTAAG 
GAGACCGAGA AGCGGTATAA AAAGATTGTT AAGTGGAAGA AAAAGGCCGA GAAATCTGGG 
AAATCGGGCA CAAAAAAGTC CTATTTCTCA GCATTCACAA ACTTCTCCGC GGAGCACATC 
GGCATGGAGA AGAAGCGGAT CCTTGCGATG TGGTTTGAGC TTTCCCTCGA AGAACGCGAG 
CAGTACGCCC GTGGAATTCC GAAAAAGTGT CCGGAAGCGG TGGATGAGAG GTTCAACCCG 
ACGTGCAAGC TTGGAGCCCT ACCGGAGAAG ATGTTGGATG AGATTGAGGG GTTCTGTACG 
CGGCGGGTGA AGAAGGTGGA TGATGAGGAG CCACCGAAGG AGGTGTTGAA GAGGACGCTG 
TATTGGAAAG GAATGAGATC CCTCGCCGAT CCAGGAGAGA ACGTCGGACT CCTCGCTGCT 
CAATCAATCG GAGAACCATC AACTCAAATG ACACTGAACA CTTTCCATTT TGCCGGTCGT 
GGAGAAATGA ACGTTACTCT AGGAATTCCG CGTCTTCGAG AAATTTTGAT GACTGCGTCG 
AAGAGCATCG CAACGCCAAG TGCATCGATT GCCGTCATCG CTGGAACATC AAGGGATCGT 
ATCGATTCGA TTAAGCGGGA GTTGGATCGA GTCTATTTGA AGCAATTGTT GAAGAATTTC 
TCGTTGGAAG AGAAGATTAC GTTGACACAA AATCAATCCT GCCGCCGCTA CCACCTCCGC 
ATCGACATTC TGGCCGCCGA GAAGCGTGAA CTCGGAGCCC GACACCTGAA ACGTTCGCAG 
ATTATGGAAG AAATCGAAAA GCGATTCATT CTCCGTGTGG CTCAGGCGAT TAAAAAGAAA 
TATCACGAGA TTACCGACTA TCAGCAAATG TCTCATCGTA CTATGCGTCA GGGAAATATG 
GCAGCGGGAA TTGAAACTGG CGGGACAAAG AATCGCGGAC TTCAGGGACC CGACAATGGA 
GATTCGTCTG ACGAGGAAGC CGACGGAGGA CGTGAAGCTG ATGCAGCTGA AGCTAGGCTT 
CATCGCAGAC ATCGAGATGA GGGGGCGGAT TATGAGGGAG AAGATGAGGA GAGAGTCGAG 
GTTCGAGAAG AAGAGGAGCC GATGGATTCG GATTCGGAAG ATGTGAAGAA GGAGGGATTG 
GATGGAGAGG ATCAGACGAC GGAGCCACTA CTGGTCAACT CGTCGAGAAT TCAATCTGTT 
CAGCGGCTTT CCGAGAATAT TTCATCGTAT ACGTATGATG TCAAGTCGAA TAAGTGGTGT 
GAAGTGGTTT TCGAGCTTCC ACTGAGGAAT AAGACCAAGA TGGATGTTTC TTCGATTGTT 
GAAAAAGAAG TCGAACTATT CATCGTCCAT CAAACTCCGG GAATCGAGCG ATGCGTCGAG 
ACGACTGAGC AGAAAAACGG AAAAGAAATG ACAATTCTGC AGACGCAAGG AGTCAATTTG 
GCTGCATTCT TCAAGCACGC AGACGTGCTC GACGTCAATT CGGTCTATTC GAACGATCTG 
AACTTGATTC TCGAGAATTA TGGAGTTGAA GCTTGCTCTA AGGCTATCAC TACTGAAATG 
AACAACGTGT TCGCCGTCTA CGGTATCGAG GTCTCGAAGC GACATCTCTC TCTAACTGCC 
GACTACATGA CATTCACCGG ACAAATCCAA CCATTCAACC GCGGAGCCAT GTCGTCGAGC 
TCATCACCCC TCCAAAAAAT GACGTTCGAA ACGACGATGG CCTTCTTGCG AGAGGCGCTC 
TTGCATGGCG AAGAAGACAA TGTGAACTCT CCGTCGGCTC GTCTCGTAAT GGGAGCCCTT 
CCGCGTGGCG GTACCGGATC GTTCGACTTG CTGCTCGACA CGAAAATGCA ATCCGAACGG 
GAGGAGCACG AGGCGGCGAG GGCTCGAAAG CGGAACGCCA AGCAGAAGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rpoa-1 ( NM_001027279.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022450.1 Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), mRNA.

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CloneID	OCa337770
Clone ID Related Accession (Same CDS sequence)	NM_001027279.3 , NM_001027279.4
Accession Version	NM_001027279.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027279.3.

ATGGATTTCT TCGTCAGAAA TGGCGAGGAG CCGTATATGC AATTCTCGAA TTTCAAGCTG 
CGGAGCTATT TTCCGCATGA AATCGACAAG CTCAGTGTGC TCAAAATCAC GCAGACAAAG 
ACTTTCGATG AGGTCGGTCA CCCGATTGCC GGCGGTCTTT ACGACCCGAT TTTGGGCCCT 
GACAACACTT TCGACATGTG CATGACGTGT AATCAATACG AACGTCATTG TCCAGGACAT 
ATGGGACATA TTCAGCTGGC GGTTCCGGTT TTCAACCCGC TTCTCTTCCA GTTCACCTAT 
AATCTTCTCA AAGGCTCCTG CGTCCACTGC CATCGATTAA CCTGCAAAGG AGACGGAGTG 
AATGCCCGAA TGCTGCTCGC CCAGCTCAGA TGCTTCGAGC TCGGCGTTGA ACACGTGGCG 
TTCGATCTGG AATCAATTCT GCGGGATAAA ATCGCAAATG CTGATCTTTT CGGCGATGAC 
GATTCGAAAA CCTTCAACGA CGTGGATGCT TGTATCGCTC AGCTCGCGAA CAAGCCGCTT 
TCGGAGCTCA CCGCGTTCAA ATCGATGCCA ACCAAGAATA GTGTTCAGCT GAAAAAAGAT 
ATTATCACCG AATTCCTCAG AAGTCACCTG TTCAAACGTC TCCAAAAATG TCCGCTCTGC 
AAGAATCGCA ACGGAGTCCT GCGAAACGAC GGAGCTCGCT CGATTCTCAT CGATTTCACC 
TCCGGGGCTC GCGGCGGCGG AAAATCCAAA AAAGCCATTA TCATCATCGG AGATAATGGA 
TATAACGAGG AATCTTCGAC AGATGAGGAT GAGGAGAAGA AGGGCGGCGG CGGCGCAGAG 
GTCACCGGAG ATGTTGTGGA TTTGGATGAA GGAAGCCTGG AGATGCAGAT GAAGAATGTG 
AGAACTGGAG AATGCGACAA ACTAGCGTGG AGAGGTGCTG AAGTCCGCGA GCACTTCAGG 
ATGCTGTTCA AAAACGACGG AAAGCTGCTG CTCAAGCTGT TCCCGATGCT TGTCGACGAG 
CTCAACGGAG AGGACATGAT CTGCCCGCTA GACGGTCTCT TCTTGGAGCG AATCCTGGTT 
CCACCGAAGA AATTCCGCCC GATCCGAATG TTCAAGGGCG CTCAATACGA GGATCCACAG 
ACTCTGAACC TTCGTAAAGT TCTCGAAGCG ACCGAGACAA TCTCGGCGAT CTCTCTAATC 
ATGAAAGGCG ACACAAGTGC TCAGTTGAAG GAGCTCATCG CGAATCGGGT CCGCGGGAAG 
ACGATCAACG CGCAGATGCA CGACGCCTAC CTGCAGCTTC AGCTTCGCGC CAACGCGATC 
TTCGATCAAG ATCTCAACAA AGGCGACAGG GATAGCATCG CCGGTATCAA GCAGATCTTG 
GAGAAGAAGC AGGGATTGTT CAGAATGCAT ATGATGGGAA AACGAGTCAA CTTTGCTTGT 
CGATCAGTCA TCACTCCGGA TCCCTACCTG GATATTGATG AGATCGGAAT TCCGGATATT 
TTCGCGAAAA AGCTCACTTT CACAGAGCCA GTGAATGCTT TTAACGTGAA CGAGATGAAG 
GGGCTTCTCC GCCAAGGCCC CCACCAGCAC CCGGGAGCCA ATTTCTTCGT GGAGCCTTCC 
GGCAAGAAGA CGATGCTCGG TGATAAGCCT GAAGAGAAGA AGCGTCGGAT GCAAATGGCC 
AAAACCCTCA ACGCGGCGAC CACAGAAAAC CTCCGCCAAA CGCCAAAAGT GTTGAGACAC 
ATGAAAAACG GCGATATGAT CATGATGAAT CGTCAGCCAT CGCTTCACAA GCCGTCGATC 
CTCGGCCACC GAGCCCGTGT GCTCACCGGA CAGCGGGCTC TTCGGATGAA CTACGCGCCT 
TGCAAGGCCT ACAATGCGGA TTTCGATGGA GACGAGATGA ACGGACACTT GGTGCAATCA 
CATATCGCGC AGACGGAAGT TCGCGAGATT GCGAACGTCG GAAGTAACTT TTTGGTGCCG 
AAGGATGCGA CACCGCTGTT GGGATTGATT CAGGATCACG TGGTGTCGGG TGTCTTGTTG 
AGTGTTCGGG ATCGATTCCT GAATAAGGAG GATTTTATGC ACCTTGTGCT CGCCTCATTC 
GCCCAGTACT CGAAGAGGAT CGAGATTCCA CCACCGACGA TTCTCTATCC GAAGCGGCTT 
TGGACTGGAA AGCAGGTCAT CACCACAATC GTCAAAAATT GTATTCCAGA CGGAAAACCG 
CTCATCAATC TCGACGGAAA AGCCAAGACA CCGCTCAGCT GCTGGATTGT GCCGGGATTC 
GATGCTCCGC AATTCGACAT GAGTGAATCT CATGTGGTGT TCAGGCAAGG AGAGCTGCTC 
GTCGGAGTTC TCGATAAGGC TCACTTTGGA GCAACGCAAT TTGGATTGGC TCATTGTGCA 
TTTGAGCTTT ATGGACATCG GTGTGGAGTG CAGTTGTTAT CGTGTTTTTC GCGGCTTTTT 
ACGACCTATT TGCAGTTCCA CGGCTTCACC CTCGGAGTCG CCGACATTCT CGTCGTCAAG 
GATGCCGACG GAAAACGCAA GGAAGCCGTC ATGGAATCCA GAACCATTGG AAATCAAGTC 
GTCAAGACAG CGTTCGGCCT TCCAGACACT GCGACGCCGG CAGAGATCAA GCGAACGCTC 
GCAGCCACCT ATTGTAATCC ACGTGGACAA GGGACTGACG TGAAAATGTT GGATTTCGGT 
ATGAAGCAAG GAATCGCCAA ATACAACGAC GCCATCACAA AATCCTGTGT GCCGACTGGT 
CTTCTCCGGC TTTTCCCACA AAATGCTCTG CAACTGATGA TTCAATCCGG AGCCAAAGGA 
TCCGCAGTCA ATGCTATTCA GATTTCAGGC TGTCTAGGCC AAATTGAGTT GGAAGGCAAG 
CGTATGGCTG TGACGATCGC CGGACGGACT CTCCCATCGT TCCGATGCTT TGATCCATCC 
CCGCGGGCAG GTGGATACAT TGATCAGCGA TTTTTGACCG GAATGAATCC ACAGGAGCTG 
TTCTTTCACA CAATGGCCGG TCGAGAAGGT CTTATTGATA CGGCTGTAAA GACTTCCCGG 
TCGGGATACC TCCAACGTTG TATCATCAAG CATTTGGAGG GAATCCGTGT GCACTACGAC 
TCGACCGTCA GGGATCATGA TGGATCGGTG ATTCAGTTTC GGTATGGAGA GGATGGAATG 
GACACGACGA AGGCGACTTT TTTGAATAAA AAGACTATGC CGTTCCTGGA AGATAATCTG 
GAAGCGGTGA CACTTGCGTC GAAGCCTGAA GGAGTTACAG ATGCTGATTT TGGAATTAAG 
GAGACCGAGA AGCGGTATAA AAAGATTGTT AAGTGGAAGA AAAAGGCCGA GAAATCTGGG 
AAATCGGGCA CAAAAAAGTC CTATTTCTCA GCATTCACAA ACTTCTCCGC GGAGCACATC 
GGCATGGAGA AGAAGCGGAT CCTTGCGATG TGGTTTGAGC TTTCCCTCGA AGAACGCGAG 
CAGTACGCCC GTGGAATTCC GAAAAAGTGT CCGGAAGCGG TGGATGAGAG GTTCAACCCG 
ACGTGCAAGC TTGGAGCCCT ACCGGAGAAG ATGTTGGATG AGATTGAGGG GTTCTGTACG 
CGGCGGGTGA AGAAGGTGGA TGATGAGGAG CCACCGAAGG AGGTGTTGAA GAGGACGCTG 
TATTGGAAAG GAATGAGATC CCTCGCCGAT CCAGGAGAGA ACGTCGGACT CCTCGCTGCT 
CAATCAATCG GAGAACCATC AACTCAAATG ACACTGAACA CTTTCCATTT TGCCGGTCGT 
GGAGAAATGA ACGTTACTCT AGGAATTCCG CGTCTTCGAG AAATTTTGAT GACTGCGTCG 
AAGAGCATCG CAACGCCAAG TGCATCGATT GCCGTCATCG CTGGAACATC AAGGGATCGT 
ATCGATTCGA TTAAGCGGGA GTTGGATCGA GTCTATTTGA AGCAATTGTT GAAGAATTTC 
TCGTTGGAAG AGAAGATTAC GTTGACACAA AATCAATCCT GCCGCCGCTA CCACCTCCGC 
ATCGACATTC TGGCCGCCGA GAAGCGTGAA CTCGGAGCCC GACACCTGAA ACGTTCGCAG 
ATTATGGAAG AAATCGAAAA GCGATTCATT CTCCGTGTGG CTCAGGCGAT TAAAAAGAAA 
TATCACGAGA TTACCGACTA TCAGCAAATG TCTCATCGTA CTATGCGTCA GGGAAATATG 
GCAGCGGGAA TTGAAACTGG CGGGACAAAG AATCGCGGAC TTCAGGGACC CGACAATGGA 
GATTCGTCTG ACGAGGAAGC CGACGGAGGA CGTGAAGCTG ATGCAGCTGA AGCTAGGCTT 
CATCGCAGAC ATCGAGATGA GGGGGCGGAT TATGAGGGAG AAGATGAGGA GAGAGTCGAG 
GTTCGAGAAG AAGAGGAGCC GATGGATTCG GATTCGGAAG ATGTGAAGAA GGAGGGATTG 
GATGGAGAGG ATCAGACGAC GGAGCCACTA CTGGTCAACT CGTCGAGAAT TCAATCTGTT 
CAGCGGCTTT CCGAGAATAT TTCATCGTAT ACGTATGATG TCAAGTCGAA TAAGTGGTGT 
GAAGTGGTTT TCGAGCTTCC ACTGAGGAAT AAGACCAAGA TGGATGTTTC TTCGATTGTT 
GAAAAAGAAG TCGAACTATT CATCGTCCAT CAAACTCCGG GAATCGAGCG ATGCGTCGAG 
ACGACTGAGC AGAAAAACGG AAAAGAAATG ACAATTCTGC AGACGCAAGG AGTCAATTTG 
GCTGCATTCT TCAAGCACGC AGACGTGCTC GACGTCAATT CGGTCTATTC GAACGATCTG 
AACTTGATTC TCGAGAATTA TGGAGTTGAA GCTTGCTCTA AGGCTATCAC TACTGAAATG 
AACAACGTGT TCGCCGTCTA CGGTATCGAG GTCTCGAAGC GACATCTCTC TCTAACTGCC 
GACTACATGA CATTCACCGG ACAAATCCAA CCATTCAACC GCGGAGCCAT GTCGTCGAGC 
TCATCACCCC TCCAAAAAAT GACGTTCGAA ACGACGATGG CCTTCTTGCG AGAGGCGCTC 
TTGCATGGCG AAGAAGACAA TGTGAACTCT CCGTCGGCTC GTCTCGTAAT GGGAGCCCTT 
CCGCGTGGCG GTACCGGATC GTTCGACTTG CTGCTCGACA CGAAAATGCA ATCCGAACGG 
GAGGAGCACG AGGCGGCGAG GGCTCGAAAG CGGAACGCCA AGCAGAAGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022450.1
CDS	7..5220
Translation	MDFFVRNGEEPYMQFSNFKLRSYFPHEIDKLSVLKITQTKTFDEVGHPIAGGLYDPILGPDNTFDMCMTCNQYERHCPGHMGHIQLAVPVFNPLLFQFTYNLLKGSCVHCHRLTCKGDGVNARMLLAQLRCFELGVEHVAFDLESILRDKIANADLFGDDDSKTFNDVDACIAQLANKPLSELTAFKSMPTKNSVQLKKDIITEFLRSHLFKRLQKCPLCKNRNGVLRNDGARSILIDFTSGARGGGKSKKAIIIIGDNGYNEESSTDEDEEKKGGGGAEVTGDVVDLDEGSLEMQMKNVRTGECDKLAWRGAEVREHFRMLFKNDGKLLLKLFPMLVDELNGEDMICPLDGLFLERILVPPKKFRPIRMFKGAQYEDPQTLNLRKVLEATETISAISLIMKGDTSAQLKELIANRVRGKTINAQMHDAYLQLQLRANAIFDQDLNKGDRDSIAGIKQILEKKQGLFRMHMMGKRVNFACRSVITPDPYLDIDEIGIPDIFAKKLTFTEPVNAFNVNEMKGLLRQGPHQHPGANFFVEPSGKKTMLGDKPEEKKRRMQMAKTLNAATTENLRQTPKVLRHMKNGDMIMMNRQPSLHKPSILGHRARVLTGQRALRMNYAPCKAYNADFDGDEMNGHLVQSHIAQTEVREIANVGSNFLVPKDATPLLGLIQDHVVSGVLLSVRDRFLNKEDFMHLVLASFAQYSKRIEIPPPTILYPKRLWTGKQVITTIVKNCIPDGKPLINLDGKAKTPLSCWIVPGFDAPQFDMSESHVVFRQGELLVGVLDKAHFGATQFGLAHCAFELYGHRCGVQLLSCFSRLFTTYLQFHGFTLGVADILVVKDADGKRKEAVMESRTIGNQVVKTAFGLPDTATPAEIKRTLAATYCNPRGQGTDVKMLDFGMKQGIAKYNDAITKSCVPTGLLRLFPQNALQLMIQSGAKGSAVNAIQISGCLGQIELEGKRMAVTIAGRTLPSFRCFDPSPRAGGYIDQRFLTGMNPQELFFHTMAGREGLIDTAVKTSRSGYLQRCIIKHLEGIRVHYDSTVRDHDGSVIQFRYGEDGMDTTKATFLNKKTMPFLEDNLEAVTLASKPEGVTDADFGIKETEKRYKKIVKWKKKAEKSGKSGTKKSYFSAFTNFSAEHIGMEKKRILAMWFELSLEEREQYARGIPKKCPEAVDERFNPTCKLGALPEKMLDEIEGFCTRRVKKVDDEEPPKEVLKRTLYWKGMRSLADPGENVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMTASKSIATPSASIAVIAGTSRDRIDSIKRELDRVYLKQLLKNFSLEEKITLTQNQSCRRYHLRIDILAAEKRELGARHLKRSQIMEEIEKRFILRVAQAIKKKYHEITDYQQMSHRTMRQGNMAAGIETGGTKNRGLQGPDNGDSSDEEADGGREADAAEARLHRRHRDEGADYEGEDEERVEVREEEEPMDSDSEDVKKEGLDGEDQTTEPLLVNSSRIQSVQRLSENISSYTYDVKSNKWCEVVFELPLRNKTKMDVSSIVEKEVELFIVHQTPGIERCVETTEQKNGKEMTILQTQGVNLAAFFKHADVLDVNSVYSNDLNLILENYGVEACSKAITTEMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGALPRGGTGSFDLLLDTKMQSEREEHEAARARKRNAKQKF

Target ORF information:

RefSeq Version	NM_001027279.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase subunit (rpoa-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027279.4

ORF Insert Sequence:

ATGGATTTCT TCGTCAGAAA TGGCGAGGAG CCGTATATGC AATTCTCGAA TTTCAAGCTG 
CGGAGCTATT TTCCGCATGA AATCGACAAG CTCAGTGTGC TCAAAATCAC GCAGACAAAG 
ACTTTCGATG AGGTCGGTCA CCCGATTGCC GGCGGTCTTT ACGACCCGAT TTTGGGCCCT 
GACAACACTT TCGACATGTG CATGACGTGT AATCAATACG AACGTCATTG TCCAGGACAT 
ATGGGACATA TTCAGCTGGC GGTTCCGGTT TTCAACCCGC TTCTCTTCCA GTTCACCTAT 
AATCTTCTCA AAGGCTCCTG CGTCCACTGC CATCGATTAA CCTGCAAAGG AGACGGAGTG 
AATGCCCGAA TGCTGCTCGC CCAGCTCAGA TGCTTCGAGC TCGGCGTTGA ACACGTGGCG 
TTCGATCTGG AATCAATTCT GCGGGATAAA ATCGCAAATG CTGATCTTTT CGGCGATGAC 
GATTCGAAAA CCTTCAACGA CGTGGATGCT TGTATCGCTC AGCTCGCGAA CAAGCCGCTT 
TCGGAGCTCA CCGCGTTCAA ATCGATGCCA ACCAAGAATA GTGTTCAGCT GAAAAAAGAT 
ATTATCACCG AATTCCTCAG AAGTCACCTG TTCAAACGTC TCCAAAAATG TCCGCTCTGC 
AAGAATCGCA ACGGAGTCCT GCGAAACGAC GGAGCTCGCT CGATTCTCAT CGATTTCACC 
TCCGGGGCTC GCGGCGGCGG AAAATCCAAA AAAGCCATTA TCATCATCGG AGATAATGGA 
TATAACGAGG AATCTTCGAC AGATGAGGAT GAGGAGAAGA AGGGCGGCGG CGGCGCAGAG 
GTCACCGGAG ATGTTGTGGA TTTGGATGAA GGAAGCCTGG AGATGCAGAT GAAGAATGTG 
AGAACTGGAG AATGCGACAA ACTAGCGTGG AGAGGTGCTG AAGTCCGCGA GCACTTCAGG 
ATGCTGTTCA AAAACGACGG AAAGCTGCTG CTCAAGCTGT TCCCGATGCT TGTCGACGAG 
CTCAACGGAG AGGACATGAT CTGCCCGCTA GACGGTCTCT TCTTGGAGCG AATCCTGGTT 
CCACCGAAGA AATTCCGCCC GATCCGAATG TTCAAGGGCG CTCAATACGA GGATCCACAG 
ACTCTGAACC TTCGTAAAGT TCTCGAAGCG ACCGAGACAA TCTCGGCGAT CTCTCTAATC 
ATGAAAGGCG ACACAAGTGC TCAGTTGAAG GAGCTCATCG CGAATCGGGT CCGCGGGAAG 
ACGATCAACG CGCAGATGCA CGACGCCTAC CTGCAGCTTC AGCTTCGCGC CAACGCGATC 
TTCGATCAAG ATCTCAACAA AGGCGACAGG GATAGCATCG CCGGTATCAA GCAGATCTTG 
GAGAAGAAGC AGGGATTGTT CAGAATGCAT ATGATGGGAA AACGAGTCAA CTTTGCTTGT 
CGATCAGTCA TCACTCCGGA TCCCTACCTG GATATTGATG AGATCGGAAT TCCGGATATT 
TTCGCGAAAA AGCTCACTTT CACAGAGCCA GTGAATGCTT TTAACGTGAA CGAGATGAAG 
GGGCTTCTCC GCCAAGGCCC CCACCAGCAC CCGGGAGCCA ATTTCTTCGT GGAGCCTTCC 
GGCAAGAAGA CGATGCTCGG TGATAAGCCT GAAGAGAAGA AGCGTCGGAT GCAAATGGCC 
AAAACCCTCA ACGCGGCGAC CACAGAAAAC CTCCGCCAAA CGCCAAAAGT GTTGAGACAC 
ATGAAAAACG GCGATATGAT CATGATGAAT CGTCAGCCAT CGCTTCACAA GCCGTCGATC 
CTCGGCCACC GAGCCCGTGT GCTCACCGGA CAGCGGGCTC TTCGGATGAA CTACGCGCCT 
TGCAAGGCCT ACAATGCGGA TTTCGATGGA GACGAGATGA ACGGACACTT GGTGCAATCA 
CATATCGCGC AGACGGAAGT TCGCGAGATT GCGAACGTCG GAAGTAACTT TTTGGTGCCG 
AAGGATGCGA CACCGCTGTT GGGATTGATT CAGGATCACG TGGTGTCGGG TGTCTTGTTG 
AGTGTTCGGG ATCGATTCCT GAATAAGGAG GATTTTATGC ACCTTGTGCT CGCCTCATTC 
GCCCAGTACT CGAAGAGGAT CGAGATTCCA CCACCGACGA TTCTCTATCC GAAGCGGCTT 
TGGACTGGAA AGCAGGTCAT CACCACAATC GTCAAAAATT GTATTCCAGA CGGAAAACCG 
CTCATCAATC TCGACGGAAA AGCCAAGACA CCGCTCAGCT GCTGGATTGT GCCGGGATTC 
GATGCTCCGC AATTCGACAT GAGTGAATCT CATGTGGTGT TCAGGCAAGG AGAGCTGCTC 
GTCGGAGTTC TCGATAAGGC TCACTTTGGA GCAACGCAAT TTGGATTGGC TCATTGTGCA 
TTTGAGCTTT ATGGACATCG GTGTGGAGTG CAGTTGTTAT CGTGTTTTTC GCGGCTTTTT 
ACGACCTATT TGCAGTTCCA CGGCTTCACC CTCGGAGTCG CCGACATTCT CGTCGTCAAG 
GATGCCGACG GAAAACGCAA GGAAGCCGTC ATGGAATCCA GAACCATTGG AAATCAAGTC 
GTCAAGACAG CGTTCGGCCT TCCAGACACT GCGACGCCGG CAGAGATCAA GCGAACGCTC 
GCAGCCACCT ATTGTAATCC ACGTGGACAA GGGACTGACG TGAAAATGTT GGATTTCGGT 
ATGAAGCAAG GAATCGCCAA ATACAACGAC GCCATCACAA AATCCTGTGT GCCGACTGGT 
CTTCTCCGGC TTTTCCCACA AAATGCTCTG CAACTGATGA TTCAATCCGG AGCCAAAGGA 
TCCGCAGTCA ATGCTATTCA GATTTCAGGC TGTCTAGGCC AAATTGAGTT GGAAGGCAAG 
CGTATGGCTG TGACGATCGC CGGACGGACT CTCCCATCGT TCCGATGCTT TGATCCATCC 
CCGCGGGCAG GTGGATACAT TGATCAGCGA TTTTTGACCG GAATGAATCC ACAGGAGCTG 
TTCTTTCACA CAATGGCCGG TCGAGAAGGT CTTATTGATA CGGCTGTAAA GACTTCCCGG 
TCGGGATACC TCCAACGTTG TATCATCAAG CATTTGGAGG GAATCCGTGT GCACTACGAC 
TCGACCGTCA GGGATCATGA TGGATCGGTG ATTCAGTTTC GGTATGGAGA GGATGGAATG 
GACACGACGA AGGCGACTTT TTTGAATAAA AAGACTATGC CGTTCCTGGA AGATAATCTG 
GAAGCGGTGA CACTTGCGTC GAAGCCTGAA GGAGTTACAG ATGCTGATTT TGGAATTAAG 
GAGACCGAGA AGCGGTATAA AAAGATTGTT AAGTGGAAGA AAAAGGCCGA GAAATCTGGG 
AAATCGGGCA CAAAAAAGTC CTATTTCTCA GCATTCACAA ACTTCTCCGC GGAGCACATC 
GGCATGGAGA AGAAGCGGAT CCTTGCGATG TGGTTTGAGC TTTCCCTCGA AGAACGCGAG 
CAGTACGCCC GTGGAATTCC GAAAAAGTGT CCGGAAGCGG TGGATGAGAG GTTCAACCCG 
ACGTGCAAGC TTGGAGCCCT ACCGGAGAAG ATGTTGGATG AGATTGAGGG GTTCTGTACG 
CGGCGGGTGA AGAAGGTGGA TGATGAGGAG CCACCGAAGG AGGTGTTGAA GAGGACGCTG 
TATTGGAAAG GAATGAGATC CCTCGCCGAT CCAGGAGAGA ACGTCGGACT CCTCGCTGCT 
CAATCAATCG GAGAACCATC AACTCAAATG ACACTGAACA CTTTCCATTT TGCCGGTCGT 
GGAGAAATGA ACGTTACTCT AGGAATTCCG CGTCTTCGAG AAATTTTGAT GACTGCGTCG 
AAGAGCATCG CAACGCCAAG TGCATCGATT GCCGTCATCG CTGGAACATC AAGGGATCGT 
ATCGATTCGA TTAAGCGGGA GTTGGATCGA GTCTATTTGA AGCAATTGTT GAAGAATTTC 
TCGTTGGAAG AGAAGATTAC GTTGACACAA AATCAATCCT GCCGCCGCTA CCACCTCCGC 
ATCGACATTC TGGCCGCCGA GAAGCGTGAA CTCGGAGCCC GACACCTGAA ACGTTCGCAG 
ATTATGGAAG AAATCGAAAA GCGATTCATT CTCCGTGTGG CTCAGGCGAT TAAAAAGAAA 
TATCACGAGA TTACCGACTA TCAGCAAATG TCTCATCGTA CTATGCGTCA GGGAAATATG 
GCAGCGGGAA TTGAAACTGG CGGGACAAAG AATCGCGGAC TTCAGGGACC CGACAATGGA 
GATTCGTCTG ACGAGGAAGC CGACGGAGGA CGTGAAGCTG ATGCAGCTGA AGCTAGGCTT 
CATCGCAGAC ATCGAGATGA GGGGGCGGAT TATGAGGGAG AAGATGAGGA GAGAGTCGAG 
GTTCGAGAAG AAGAGGAGCC GATGGATTCG GATTCGGAAG ATGTGAAGAA GGAGGGATTG 
GATGGAGAGG ATCAGACGAC GGAGCCACTA CTGGTCAACT CGTCGAGAAT TCAATCTGTT 
CAGCGGCTTT CCGAGAATAT TTCATCGTAT ACGTATGATG TCAAGTCGAA TAAGTGGTGT 
GAAGTGGTTT TCGAGCTTCC ACTGAGGAAT AAGACCAAGA TGGATGTTTC TTCGATTGTT 
GAAAAAGAAG TCGAACTATT CATCGTCCAT CAAACTCCGG GAATCGAGCG ATGCGTCGAG 
ACGACTGAGC AGAAAAACGG AAAAGAAATG ACAATTCTGC AGACGCAAGG AGTCAATTTG 
GCTGCATTCT TCAAGCACGC AGACGTGCTC GACGTCAATT CGGTCTATTC GAACGATCTG 
AACTTGATTC TCGAGAATTA TGGAGTTGAA GCTTGCTCTA AGGCTATCAC TACTGAAATG 
AACAACGTGT TCGCCGTCTA CGGTATCGAG GTCTCGAAGC GACATCTCTC TCTAACTGCC 
GACTACATGA CATTCACCGG ACAAATCCAA CCATTCAACC GCGGAGCCAT GTCGTCGAGC 
TCATCACCCC TCCAAAAAAT GACGTTCGAA ACGACGATGG CCTTCTTGCG AGAGGCGCTC 
TTGCATGGCG AAGAAGACAA TGTGAACTCT CCGTCGGCTC GTCTCGTAAT GGGAGCCCTT 
CCGCGTGGCG GTACCGGATC GTTCGACTTG CTGCTCGACA CGAAAATGCA ATCCGAACGG 
GAGGAGCACG AGGCGGCGAG GGCTCGAAAG CGGAACGCCA AGCAGAAGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rpoa-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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