gcn-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gcn-2
Entrez Gene ID	174953
Full Name	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001267442.3	NP_001254371.2	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
NM_001267441.2	NP_001254370.2	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
NM_001267442.2	NP_001254371.2	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
NM_001267441.3	NP_001254370.2	Eukaryotic translation initiation factor 2-alpha kinase gcn-2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GCN-2 dependent inhibition of protein synthesis activates osmosensitive gene transcription via WNK and Ste20 kinase signaling
Title: GCN-2 dependent inhibition of protein synthesis activates osmosensitive gene transcription via WNK and Ste20 kinase signaling.

The experiments described here implicate the eIF2alpha kinase GCN-2 in the maintenance of mitochondrial function and protein homeostasis.
Title: Protective coupling of mitochondrial function and protein synthesis via the eIF2? kinase GCN-2.

The following gcn-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcn-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337754	NM_001267442.3 Latest version!	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa337755	NM_001267441.3 Latest version!	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa337754	NM_001267442.2	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa337755	NM_001267441.2	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcn-2 ( NM_001267442.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254371.2 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.

Price & Availability↑

CloneID	OCa337754
Clone ID Related Accession (Same CDS sequence)	NM_001267442.3 , NM_001267442.2
Accession Version	NM_001267442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267442.2.

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAAAGTTCGG TATCAACAAC AGTGGCGACG 
ACGAGCATCC GACCGATCAG TGCGACGGTG GCGAATCTCA ATGTGATCCT TGTCACGTCG 
GCCGACAGAT TCCACAAAGT TATGAAGGAG AAGAAACGAG TGGAGAGTCA AGTCAGGAAC 
CATCTCACAG AATTTGTGGC TCACTTCACG AGCAAGACCA GGATCGAGGT GTTGGTGTGT 
GATATTCCGG CTGATGTGAT CAAGAAGATC GTTAGCGAGC TCACGAAGAC TAGTTCAGAA 
GCTGAGATCG ACAAGCTATT CGATCAGCTG ATCCAGAAAC ATGGAAAAGT CGACCTGTCA 
CCGCTCCGAA GACAATTTCA CATCACTCTC AATGGCATTT CGACGGGCTC TGCGCAAGTG 
GCCATTCTCT TTTACCGGCA ATCGGACAAT TTCTACCGTT ATCTCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254371.2
CDS	137..5227
Translation	MTKENQIVLDERVKENQHLQEEEKLALDAVYLNQITYIKAHWHVWVPTNCHILLKALDSCFLNGDPLGKSKLSVILHVKCSEDYPQRKPAVDLLDPQGLSKEDVQNLLTILRQMADTWEGCVVIAELAHRVREFLTDHTPRPAGSFHDDMLANKVRTEAEKQRKRLDTEQKELELLEEEMRQRNAIEMEKTLNGTRQENETRIIGGRRIVVLSNMPNTQLLISEWTFRFSSNRNPAEGKRKDFAPFLQKLDAVYNEIQKLCEIKGLDQNLVEYAFVHLQKISVSPDQILIQLNVAQKIFSSEENMQDTYELIVQKSNLLRLLAAQAICGLRYLHEASMTHKHLTLGSVWTRNSTGDCVFRFSDFGSMGPLLDLVKMFGDICSGKYVARDEDKEKEYDRRRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENIFTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSDDDSSLGAVPIPGKEKIGKKGKLKTGKSLEDKENKANLGGGDSLMPMNLRGLVKDHSIGVDAKEWSTPFGKPEGPKCASRMRQSKRSTPSGGLKHLSECSSDDEDDDDSSEIDWDAESEEVEDEESDDSDEEDEDDGERLVQLNTETSTGADSVFERSTADEDVVFTAESEDLNAKRRESIELMEINTTTTSKSKLAIDVVPVRKPRILCIQMEYCDRATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRDDIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNNREVLVETLRAEFCKILKIHSFEKLHTHTLMPVSTALAAASVRTKPVEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSANGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPGMKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLIPVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRPRTFGDGLLFQIQVEKPSTIANNKRGRRQNVLAGGRYDSALLRERHPRDFVYEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVFHIPVDDLESLTEHRNRASITHILAVYNTLNEVICKTETSSETMDVDSAISSVWRGVQALDGQSIHMTPCGGGPISSISTPGEAHHHDDHHPGTPVIASKSSVSTTVATTSIRPISATVANLNVILVTSADRFHKVMKEKKRVESQVRNHLTEFVAHFTSKTRIEVLVCDIPADVIKKIVSELTKTSSEAEIDKLFDQLIQKHGKVDLSPLRRQFHITLNGISTGSAQVAILFYRQSDNFYRYLV

Target ORF information:

RefSeq Version	NM_001267442.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267442.3

ORF Insert Sequence:

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAAAGTTCGG TATCAACAAC AGTGGCGACG 
ACGAGCATCC GACCGATCAG TGCGACGGTG GCGAATCTCA ATGTGATCCT TGTCACGTCG 
GCCGACAGAT TCCACAAAGT TATGAAGGAG AAGAAACGAG TGGAGAGTCA AGTCAGGAAC 
CATCTCACAG AATTTGTGGC TCACTTCACG AGCAAGACCA GGATCGAGGT GTTGGTGTGT 
GATATTCCGG CTGATGTGAT CAAGAAGATC GTTAGCGAGC TCACGAAGAC TAGTTCAGAA 
GCTGAGATCG ACAAGCTATT CGATCAGCTG ATCCAGAAAC ATGGAAAAGT CGACCTGTCA 
CCGCTCCGAA GACAATTTCA CATCACTCTC AATGGCATTT CGACGGGCTC TGCGCAAGTG 
GCCATTCTCT TTTACCGGCA ATCGGACAAT TTCTACCGTT ATCTCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcn-2 ( NM_001267442.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254371.2 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.

Price & Availability↑

CloneID	OCa337754
Clone ID Related Accession (Same CDS sequence)	NM_001267442.3 , NM_001267442.2
Accession Version	NM_001267442.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Feb 27, 2013 this sequence version replaced NM_001267442.1. COMPLETENESS: incomplete on both ends.

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAAAGTTCGG TATCAACAAC AGTGGCGACG 
ACGAGCATCC GACCGATCAG TGCGACGGTG GCGAATCTCA ATGTGATCCT TGTCACGTCG 
GCCGACAGAT TCCACAAAGT TATGAAGGAG AAGAAACGAG TGGAGAGTCA AGTCAGGAAC 
CATCTCACAG AATTTGTGGC TCACTTCACG AGCAAGACCA GGATCGAGGT GTTGGTGTGT 
GATATTCCGG CTGATGTGAT CAAGAAGATC GTTAGCGAGC TCACGAAGAC TAGTTCAGAA 
GCTGAGATCG ACAAGCTATT CGATCAGCTG ATCCAGAAAC ATGGAAAAGT CGACCTGTCA 
CCGCTCCGAA GACAATTTCA CATCACTCTC AATGGCATTT CGACGGGCTC TGCGCAAGTG 
GCCATTCTCT TTTACCGGCA ATCGGACAAT TTCTACCGTT ATCTCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254371.2
CDS	1..5091
Translation	MTKENQIVLDERVKENQHLQEEEKLALDAVYLNQITYIKAHWHVWVPTNCHILLKALDSCFLNGDPLGKSKLSVILHVKCSEDYPQRKPAVDLLDPQGLSKEDVQNLLTILRQMADTWEGCVVIAELAHRVREFLTDHTPRPAGSFHDDMLANKVRTEAEKQRKRLDTEQKELELLEEEMRQRNAIEMEKTLNGTRQENETRIIGGRRIVVLSNMPNTQLLISEWTFRFSSNRNPAEGKRKDFAPFLQKLDAVYNEIQKLCEIKGLDQNLVEYAFVHLQKISVSPDQILIQLNVAQKIFSSEENMQDTYELIVQKSNLLRLLAAQAICGLRYLHEASMTHKHLTLGSVWTRNSTGDCVFRFSDFGSMGPLLDLVKMFGDICSGKYVARDEDKEKEYDRRRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENIFTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSDDDSSLGAVPIPGKEKIGKKGKLKTGKSLEDKENKANLGGGDSLMPMNLRGLVKDHSIGVDAKEWSTPFGKPEGPKCASRMRQSKRSTPSGGLKHLSECSSDDEDDDDSSEIDWDAESEEVEDEESDDSDEEDEDDGERLVQLNTETSTGADSVFERSTADEDVVFTAESEDLNAKRRESIELMEINTTTTSKSKLAIDVVPVRKPRILCIQMEYCDRATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRDDIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNNREVLVETLRAEFCKILKIHSFEKLHTHTLMPVSTALAAASVRTKPVEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSANGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPGMKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLIPVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRPRTFGDGLLFQIQVEKPSTIANNKRGRRQNVLAGGRYDSALLRERHPRDFVYEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVFHIPVDDLESLTEHRNRASITHILAVYNTLNEVICKTETSSETMDVDSAISSVWRGVQALDGQSIHMTPCGGGPISSISTPGEAHHHDDHHPGTPVIASKSSVSTTVATTSIRPISATVANLNVILVTSADRFHKVMKEKKRVESQVRNHLTEFVAHFTSKTRIEVLVCDIPADVIKKIVSELTKTSSEAEIDKLFDQLIQKHGKVDLSPLRRQFHITLNGISTGSAQVAILFYRQSDNFYRYLV

Target ORF information:

RefSeq Version	NM_001267442.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267442.2

ORF Insert Sequence:

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAAAGTTCGG TATCAACAAC AGTGGCGACG 
ACGAGCATCC GACCGATCAG TGCGACGGTG GCGAATCTCA ATGTGATCCT TGTCACGTCG 
GCCGACAGAT TCCACAAAGT TATGAAGGAG AAGAAACGAG TGGAGAGTCA AGTCAGGAAC 
CATCTCACAG AATTTGTGGC TCACTTCACG AGCAAGACCA GGATCGAGGT GTTGGTGTGT 
GATATTCCGG CTGATGTGAT CAAGAAGATC GTTAGCGAGC TCACGAAGAC TAGTTCAGAA 
GCTGAGATCG ACAAGCTATT CGATCAGCTG ATCCAGAAAC ATGGAAAAGT CGACCTGTCA 
CCGCTCCGAA GACAATTTCA CATCACTCTC AATGGCATTT CGACGGGCTC TGCGCAAGTG 
GCCATTCTCT TTTACCGGCA ATCGGACAAT TTCTACCGTT ATCTCGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcn-2 ( NM_001267441.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254370.2 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.

Price & Availability↑

CloneID	OCa337755
Clone ID Related Accession (Same CDS sequence)	NM_001267441.2 , NM_001267441.3
Accession Version	NM_001267441.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Feb 27, 2013 this sequence version replaced NM_001267441.1. COMPLETENESS: incomplete on both ends.

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAATGTTTCA GAAGTTCGGT ATCAACAACA 
GTGGCGACGA CGAGCATCCG ACCGATCAGT GCGACGGTGG CGAATCTCAA TGTGATCCTT 
GTCACGTCGG CCGACAGATT CCACAAAGTT ATGAAGGAGA AGAAACGAGT GGAGAGTCAA 
GTCAGGAACC ATCTCACAGA ATTTGTGGCT CACTTCACGA GCAAGACCAG GATCGAGGTG 
TTGGTGTGTG ATATTCCGGC TGATGTGATC AAGAAGATCG TTAGCGAGCT CACGAAGACT 
AGTTCAGAAG CTGAGATCGA CAAGCTATTC GATCAGCTGA TCCAGAAACA TGGAAAAGTC 
GACCTGTCAC CGCTCCGAAG ACAATTTCAC ATCACTCTCA ATGGCATTTC GACGGGCTCT 
GCGCAAGTGG CCATTCTCTT TTACCGGCAA TCGGACAATT TCTACCGTTA TCTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254370.2
CDS	1..5100
Translation	MTKENQIVLDERVKENQHLQEEEKLALDAVYLNQITYIKAHWHVWVPTNCHILLKALDSCFLNGDPLGKSKLSVILHVKCSEDYPQRKPAVDLLDPQGLSKEDVQNLLTILRQMADTWEGCVVIAELAHRVREFLTDHTPRPAGSFHDDMLANKVRTEAEKQRKRLDTEQKELELLEEEMRQRNAIEMEKTLNGTRQENETRIIGGRRIVVLSNMPNTQLLISEWTFRFSSNRNPAEGKRKDFAPFLQKLDAVYNEIQKLCEIKGLDQNLVEYAFVHLQKISVSPDQILIQLNVAQKIFSSEENMQDTYELIVQKSNLLRLLAAQAICGLRYLHEASMTHKHLTLGSVWTRNSTGDCVFRFSDFGSMGPLLDLVKMFGDICSGKYVARDEDKEKEYDRRRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENIFTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSDDDSSLGAVPIPGKEKIGKKGKLKTGKSLEDKENKANLGGGDSLMPMNLRGLVKDHSIGVDAKEWSTPFGKPEGPKCASRMRQSKRSTPSGGLKHLSECSSDDEDDDDSSEIDWDAESEEVEDEESDDSDEEDEDDGERLVQLNTETSTGADSVFERSTADEDVVFTAESEDLNAKRRESIELMEINTTTTSKSKLAIDVVPVRKPRILCIQMEYCDRATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRDDIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNNREVLVETLRAEFCKILKIHSFEKLHTHTLMPVSTALAAASVRTKPVEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSANGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPGMKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLIPVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRPRTFGDGLLFQIQVEKPSTIANNKRGRRQNVLAGGRYDSALLRERHPRDFVYEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVFHIPVDDLESLTEHRNRASITHILAVYNTLNEVICKTETSSETMDVDSAISSVWRGVQALDGQSIHMTPCGGGPISSISTPGEAHHHDDHHPGTPVIASKCFRSSVSTTVATTSIRPISATVANLNVILVTSADRFHKVMKEKKRVESQVRNHLTEFVAHFTSKTRIEVLVCDIPADVIKKIVSELTKTSSEAEIDKLFDQLIQKHGKVDLSPLRRQFHITLNGISTGSAQVAILFYRQSDNFYRYLV

Target ORF information:

RefSeq Version	NM_001267441.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267441.2

ORF Insert Sequence:

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAATGTTTCA GAAGTTCGGT ATCAACAACA 
GTGGCGACGA CGAGCATCCG ACCGATCAGT GCGACGGTGG CGAATCTCAA TGTGATCCTT 
GTCACGTCGG CCGACAGATT CCACAAAGTT ATGAAGGAGA AGAAACGAGT GGAGAGTCAA 
GTCAGGAACC ATCTCACAGA ATTTGTGGCT CACTTCACGA GCAAGACCAG GATCGAGGTG 
TTGGTGTGTG ATATTCCGGC TGATGTGATC AAGAAGATCG TTAGCGAGCT CACGAAGACT 
AGTTCAGAAG CTGAGATCGA CAAGCTATTC GATCAGCTGA TCCAGAAACA TGGAAAAGTC 
GACCTGTCAC CGCTCCGAAG ACAATTTCAC ATCACTCTCA ATGGCATTTC GACGGGCTCT 
GCGCAAGTGG CCATTCTCTT TTACCGGCAA TCGGACAATT TCTACCGTTA TCTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcn-2 ( NM_001267441.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254370.2 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.

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CloneID	OCa337755
Clone ID Related Accession (Same CDS sequence)	NM_001267441.2 , NM_001267441.3
Accession Version	NM_001267441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase gcn-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267441.2.

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAATGTTTCA GAAGTTCGGT ATCAACAACA 
GTGGCGACGA CGAGCATCCG ACCGATCAGT GCGACGGTGG CGAATCTCAA TGTGATCCTT 
GTCACGTCGG CCGACAGATT CCACAAAGTT ATGAAGGAGA AGAAACGAGT GGAGAGTCAA 
GTCAGGAACC ATCTCACAGA ATTTGTGGCT CACTTCACGA GCAAGACCAG GATCGAGGTG 
TTGGTGTGTG ATATTCCGGC TGATGTGATC AAGAAGATCG TTAGCGAGCT CACGAAGACT 
AGTTCAGAAG CTGAGATCGA CAAGCTATTC GATCAGCTGA TCCAGAAACA TGGAAAAGTC 
GACCTGTCAC CGCTCCGAAG ACAATTTCAC ATCACTCTCA ATGGCATTTC GACGGGCTCT 
GCGCAAGTGG CCATTCTCTT TTACCGGCAA TCGGACAATT TCTACCGTTA TCTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254370.2
CDS	137..5236
Translation	MTKENQIVLDERVKENQHLQEEEKLALDAVYLNQITYIKAHWHVWVPTNCHILLKALDSCFLNGDPLGKSKLSVILHVKCSEDYPQRKPAVDLLDPQGLSKEDVQNLLTILRQMADTWEGCVVIAELAHRVREFLTDHTPRPAGSFHDDMLANKVRTEAEKQRKRLDTEQKELELLEEEMRQRNAIEMEKTLNGTRQENETRIIGGRRIVVLSNMPNTQLLISEWTFRFSSNRNPAEGKRKDFAPFLQKLDAVYNEIQKLCEIKGLDQNLVEYAFVHLQKISVSPDQILIQLNVAQKIFSSEENMQDTYELIVQKSNLLRLLAAQAICGLRYLHEASMTHKHLTLGSVWTRNSTGDCVFRFSDFGSMGPLLDLVKMFGDICSGKYVARDEDKEKEYDRRRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGNFIAKCQEAKNIDQLVEDPFLKEECQSESENIFTPFGGAMSPDGRMLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMVRYYYAWAEDLIPIVEETSDDDSSLGAVPIPGKEKIGKKGKLKTGKSLEDKENKANLGGGDSLMPMNLRGLVKDHSIGVDAKEWSTPFGKPEGPKCASRMRQSKRSTPSGGLKHLSECSSDDEDDDDSSEIDWDAESEEVEDEESDDSDEEDEDDGERLVQLNTETSTGADSVFERSTADEDVVFTAESEDLNAKRRESIELMEINTTTTSKSKLAIDVVPVRKPRILCIQMEYCDRATLRQYIDENHCFNAPTEVWRIFSEVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAMLSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSMDRVSTLNNLRDDIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKEDATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNNREVLVETLRAEFCKILKIHSFEKLHTHTLMPVSTALAAASVRTKPVEVLDRSGVPVALPMDLRQNFVRFCVRNSVQRMKRFNFGRVYSQTSANGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSLPGMKFTLKIGHAQLIEAQIRHLKLSDDVRAELLDALHLISVSDRPHSHKEKMDMLTPKIGAKAANIITKLLIPVEDNFGAFKEKVACFRKKLKVDAARVLVDKAIRDLEEIVGTFKFCRTEAIEQISIVYDSQTCYRPRTFGDGLLFQIQVEKPSTIANNKRGRRQNVLAGGRYDSALLRERHPRDFVYEIPLCISGFGVAMDVVSQIRDSINKSANIPKTPQNHCKVLICSMVQPDGSNLITQKFELAKKLWSMGIEADVFHIPVDDLESLTEHRNRASITHILAVYNTLNEVICKTETSSETMDVDSAISSVWRGVQALDGQSIHMTPCGGGPISSISTPGEAHHHDDHHPGTPVIASKCFRSSVSTTVATTSIRPISATVANLNVILVTSADRFHKVMKEKKRVESQVRNHLTEFVAHFTSKTRIEVLVCDIPADVIKKIVSELTKTSSEAEIDKLFDQLIQKHGKVDLSPLRRQFHITLNGISTGSAQVAILFYRQSDNFYRYLV

Target ORF information:

RefSeq Version	NM_001267441.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase gcn-2 (gcn-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267441.3

ORF Insert Sequence:

ATGACCAAGG AGAATCAAAT AGTACTTGAC GAACGGGTAA AGGAGAACCA GCATCTGCAG 
GAGGAAGAAA AACTAGCGTT AGACGCCGTT TATTTGAATC AGATTACTTA CATCAAAGCT 
CATTGGCATG TCTGGGTGCC CACAAACTGT CACATCCTGT TAAAAGCCCT CGATTCGTGC 
TTTTTGAATG GAGACCCACT TGGAAAAAGT AAATTGAGCG TTATTCTCCA TGTAAAGTGC 
TCCGAAGACT ATCCACAAAG GAAGCCAGCC GTGGATCTTC TCGATCCTCA AGGACTTTCG 
AAGGAAGATG TTCAAAATTT GCTAACTATC CTTCGACAAA TGGCAGACAC GTGGGAAGGA 
TGTGTTGTGA TCGCCGAACT GGCGCACAGG GTCAGAGAAT TTCTAACGGA CCATACTCCC 
CGCCCCGCTG GAAGTTTCCA TGATGATATG TTGGCAAACA AGGTCAGGAC AGAGGCCGAA 
AAACAAAGAA AACGACTCGA CACGGAGCAA AAAGAGCTGG AGTTGTTGGA GGAGGAGATG 
CGACAGAGAA ACGCGATTGA AATGGAGAAA ACGTTGAATG GCACACGACA GGAGAATGAA 
ACGCGAATAA TTGGAGGACG GCGAATCGTT GTACTCTCCA ATATGCCTAA CACCCAACTT 
CTCATTTCCG AATGGACGTT CCGATTCTCC AGCAATCGAA ATCCGGCTGA AGGGAAGCGA 
AAAGATTTCG CACCGTTCCT ACAGAAGCTG GACGCAGTGT ACAATGAGAT TCAGAAGCTT 
TGCGAAATTA AAGGCCTTGA TCAGAATCTG GTGGAATATG CATTTGTGCA TTTGCAGAAG 
ATCAGTGTTT CACCGGATCA GATTCTGATT CAACTGAACG TGGCTCAGAA GATATTCTCA 
TCCGAGGAAA ATATGCAGGA CACCTATGAG CTGATTGTTC AGAAATCGAA TCTTCTCCGA 
TTACTCGCAG CACAAGCCAT CTGCGGTCTC CGATATCTTC ACGAAGCTTC CATGACACAC 
AAGCATCTGA CATTAGGAAG TGTGTGGACC CGAAACAGTA CAGGCGACTG TGTTTTTAGA 
TTCTCGGATT TCGGCTCAAT GGGACCCTTG CTGGATTTAG TTAAGATGTT CGGAGATATT 
TGCTCGGGAA AGTATGTTGC ACGCGACGAG GATAAAGAGA AGGAGTATGA TCGACGACGG 
AAAGATCTTT TCCAACTAGG AACACTCCTA GACGGTTTGA TACTCGCTAC TCGTGGATCT 
ACCTACTCTC GAGTTCCAAC TCCAGTCGAA GGTAATCAGA ACACTGGTAC GAACCTCCTC 
GGCAACTTTA TCGCAAAATG CCAGGAAGCG AAAAATATTG ATCAACTCGT TGAAGATCCA 
TTTTTAAAAG AAGAATGCCA ATCTGAAAGC GAAAACATCT TCACTCCATT CGGTGGAGCA 
ATGAGCCCTG ATGGAAGAAT GCTCGCTGAT AACGTGATTA TTCGAGTTCT TGGGAGAGGC 
GGTTTCGGTG ATGTCGTTCT TGTTCGAAAT AAGATGGACA GTACGGATTA TGCAATTAAA 
CGGATCCCTT TGAACGCGAA AAGTGATAAA TTGAATCGAA AAATCGCAAA AGAGGCGAAA 
TTCTTCGCAA AATTGAATCA TCCGAATATG GTTCGATACT ACTATGCTTG GGCTGAAGAC 
TTGATTCCGA TCGTTGAAGA AACGTCTGAT GATGATTCCT CGCTTGGAGC TGTTCCTATT 
CCGGGAAAAG AGAAAATTGG CAAGAAGGGA AAACTGAAGA CAGGAAAGTC GCTAGAAGAT 
AAAGAGAACA AAGCTAATCT TGGTGGCGGC GATTCGTTGA TGCCTATGAA TTTGAGAGGA 
CTCGTCAAAG ATCACAGCAT TGGAGTTGAT GCGAAAGAAT GGTCGACACC ATTTGGAAAA 
CCGGAGGGAC CTAAATGTGC TTCACGAATG AGACAGAGCA AGAGAAGCAC ACCCAGTGGA 
GGACTGAAGC ATCTATCCGA GTGCTCATCT GATGACGAGG ATGATGATGA TAGTAGTGAG 
ATTGATTGGG ATGCTGAAAG TGAAGAGGTG GAGGATGAAG AATCAGACGA TTCGGATGAA 
GAGGATGAGG ATGATGGTGA ACGACTTGTT CAGCTGAACA CCGAGACTTC TACTGGAGCT 
GATAGTGTAT TTGAGAGATC CACTGCTGAT GAAGATGTTG TGTTCACCGC TGAATCGGAA 
GATTTGAATG CAAAAAGGCG GGAGTCGATT GAGCTAATGG AAATAAACAC GACGACGACG 
AGCAAGAGCA AGTTAGCGAT TGATGTTGTT CCAGTGAGAA AGCCGCGAAT CTTGTGCATC 
CAGATGGAGT ACTGTGACCG TGCCACTCTT CGTCAGTATA TTGATGAAAA TCACTGCTTC 
AACGCGCCCA CGGAAGTTTG GAGAATCTTT TCGGAAGTTT TGTGTGGTCT CAAATACATG 
CATGACATGG CAATGATTCA TCGGGATATC AAGCCATTGA ACATCTTCTT AACGTCACAA 
AATGGTGTGA AGATCGGAGA TTTCGGTCTG GCAACGCTGG AAGCAATGAG CTCAAAGGGA 
AAAATCGTCG GTGGTGCCGC CGAGAAAAGC ACTAGTATTG AAGCGATGCT TTCTCCGAAT 
GGAGTGAAGA GTAAAGGATC AGATGTTCAT CAAACAAGAG ATATTGGAAC TCAACTCTAC 
ATGGCTCCAG AGCTCTTTGT CGATGAGCTG GTTCACAAGG CACCGTATAC TTCAAAGATT 
GATATCTACA GTGCAGGAGT TGTTCTATTT GAGATGTTCT ATCGTCCCTT GCCACCAAGT 
ATGGATCGAG TTTCGACGCT CAACAATCTC CGAGACGATA TCAAGATTCC CAGTGATTTT 
GGTGCTGGAC TTGCTGCTCC GATGGCTGGG CTCGCGAGAA GAACTGTGGA GAAGATGCTT 
CAACGGAATC CGGATGAAAG ACCAACAGCT GACGATCTTC TGAACGATGA GGATCTTCCG 
ATGCATACAA AGGAAGATGC GACGTTCCGG AATCTTTGTG AGAAAGTTAT CAAAAAGCGA 
GATGGTCGAA TGAATGCGTG GCTACTCGAT AAACAATTCA AAGAGGAAGT TCCGACGTCT 
CTGAACTATT GTTACGATGT GGATATTTGT CTTGAGCGGG CCAAGTACAA CAATCGGGAG 
GTGCTCGTCG AGACTCTTCG TGCCGAATTT TGCAAGATTC TCAAAATCCA CTCTTTCGAA 
AAATTGCATA CTCATACGTT GATGCCGGTG TCGACAGCGC TAGCCGCTGC ATCGGTGCGC 
ACGAAGCCCG TCGAGGTACT GGATAGAAGT GGAGTTCCGG TGGCACTTCC GATGGATTTG 
CGGCAGAATT TCGTTCGATT CTGTGTGAGA AATAGTGTGC AGCGAATGAA ACGATTCAAT 
TTCGGACGAG TCTACTCACA AACATCGGCG AACGGGCATC CACATGAGAG ATGGGAGTGT 
TGTGTTGATT GTATTGGACC GCAGTGTTCT TCGCCTTCTC TTGAGGCTGA GCTGCTGCTC 
GTTGCATGTG AAATGATGAT TGGCTCACTG CCCGGAATGA AGTTTACTCT GAAAATCGGG 
CACGCACAGC TCATCGAGGC TCAAATTCGC CACCTGAAAC TATCGGATGA TGTTCGTGCT 
GAGCTCCTTG ATGCTCTTCA CCTCATCTCT GTATCTGATC GACCACATTC TCACAAGGAA 
AAGATGGATA TGCTGACGCC GAAGATCGGA GCCAAGGCCG CGAACATAAT CACGAAACTT 
TTGATTCCGG TGGAGGATAA TTTCGGCGCG TTCAAGGAGA AAGTAGCATG TTTCCGCAAG 
AAACTAAAAG TCGACGCAGC TCGTGTGCTC GTTGACAAAG CAATCCGGGA TTTGGAAGAG 
ATTGTCGGAA CCTTCAAGTT TTGTCGAACT GAAGCAATTG AGCAGATTAG CATTGTGTAC 
GACTCGCAGA CCTGTTACCG TCCTCGAACA TTTGGAGACG GACTCCTTTT CCAGATTCAA 
GTGGAAAAAC CGAGCACGAT TGCGAACAAC AAGAGGGGTC GGCGCCAAAA TGTTTTGGCT 
GGTGGTCGAT ACGATTCGGC ACTTCTTCGA GAACGACATC CACGAGATTT TGTATACGAA 
ATTCCTCTGT GCATTTCGGG ATTTGGTGTT GCGATGGATG TGGTCTCACA GATACGAGAC 
TCGATCAACA AATCTGCGAA TATCCCGAAA ACGCCTCAAA ATCATTGCAA AGTACTAATT 
TGTTCGATGG TGCAGCCTGA CGGTAGCAAT TTGATCACTC AGAAGTTTGA GCTTGCAAAA 
AAGTTGTGGA GCATGGGAAT TGAAGCCGAT GTGTTCCATA TTCCAGTGGA CGATCTTGAA 
TCGCTAACGG AACATCGCAA CCGAGCTTCA ATAACACACA TTCTTGCCGT CTATAACACA 
CTTAACGAGG TCATTTGCAA AACGGAAACA TCTTCGGAAA CGATGGACGT GGACTCGGCG 
ATTTCAAGTG TGTGGCGAGG AGTCCAAGCT CTTGATGGTC AAAGTATTCA TATGACTCCA 
TGTGGTGGTG GACCGATCTC TTCAATAAGC ACTCCTGGAG AAGCTCATCA TCATGATGAT 
CATCACCCGG GAACTCCTGT TATTGCGTCC AAATGTTTCA GAAGTTCGGT ATCAACAACA 
GTGGCGACGA CGAGCATCCG ACCGATCAGT GCGACGGTGG CGAATCTCAA TGTGATCCTT 
GTCACGTCGG CCGACAGATT CCACAAAGTT ATGAAGGAGA AGAAACGAGT GGAGAGTCAA 
GTCAGGAACC ATCTCACAGA ATTTGTGGCT CACTTCACGA GCAAGACCAG GATCGAGGTG 
TTGGTGTGTG ATATTCCGGC TGATGTGATC AAGAAGATCG TTAGCGAGCT CACGAAGACT 
AGTTCAGAAG CTGAGATCGA CAAGCTATTC GATCAGCTGA TCCAGAAACA TGGAAAAGTC 
GACCTGTCAC CGCTCCGAAG ACAATTTCAC ATCACTCTCA ATGGCATTTC GACGGGCTCT 
GCGCAAGTGG CCATTCTCTT TTACCGGCAA TCGGACAATT TCTACCGTTA TCTCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gcn-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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