ash-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ash-2
Entrez Gene ID	174838
Full Name	ASH histone methyltransferase complex subunit (Drosophila absent, small or homeotic discs)
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064152.3	NP_496553.1	Set1/Ash2 histone methyltransferase complex subunit ash-2
NM_001330962.2	NP_001317781.1	B30.2/SPRY domain-containing protein
NM_064154.1	NP_496555.1	Set1/Ash2 histone methyltransferase complex subunit ash-2
NM_001373798.1	NP_001360415.1	B30.2/SPRY domain-containing protein
NM_001330961.1	NP_001317780.1	Set1/Ash2 histone methyltransferase complex subunit ash-2
NM_064154.2	NP_496555.1	Set1/Ash2 histone methyltransferase complex subunit ash-2
NM_001330962.1	NP_001317781.1	Set1/Ash2 histone methyltransferase complex subunit ash-2
NM_001373799.1	NP_001359515.1	B30.2/SPRY domain-containing protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ASH2L	NP_001026566.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003705	XP_562582.3
Schizosaccharomyces pombe (fission yeast)	ash2	NP_596557.1
Mus musculus (house mouse)	Ash2l	NP_035921.2
Danio rerio (zebrafish)	ash2l	NP_001103575.2
Drosophila melanogaster (fruit fly)	ash2	NP_788735.1
Pan troglodytes (chimpanzee)	ASH2L	XP_001170172.1
	ASH2L	XP_002805359.1
Canis lupus familiaris (dog)	ASH2L	XP_005629916.1
Rattus norvegicus (Norway rat)	Ash2l	NP_001099559.1
Xenopus tropicalis (tropical clawed frog)	ash2l	XP_002932768.2
Homo sapiens (human)	ASH2L	NP_004665.2
Bos taurus (cattle)	ASH2L	NP_001192402.1
Caenorhabditis elegans (roundworm)	ash-2	NP_496553.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In AIB interneurons, NPR-4 was involved in the control of preference choice by activating the functions of ASH-2 trithorax complex consisting of SET-2, ASH-2, and WDR-5, implying the crucial role of molecular machinery of trimethylation of histone H3K4 in the preference choice control.
Title: FLP-4 neuropeptide and its receptor in a neuronal circuit regulate preference choice through functions of ASH-2 trithorax complex in Caenorhabditis elegans.

SET-2 is responsible for the majority of bulk H3K4 methylation at all developmental stages
Title: Caenorhabditis elegans chromatin-associated proteins SET-2 and ASH-2 are differentially required for histone H3 Lys 4 methylation in embryos and adult germ cells.

The following ash-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ash-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338024	NM_064154.2 Latest version!	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338032	NM_064152.3 Latest version!	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa338032	NM_001373799.1 Latest version!	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338166	NM_001373798.1 Latest version!	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338166	NM_001330961.1 Latest version!	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa338167	NM_001330962.2 Latest version!	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCa338024	NM_064154.1	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa338167	NM_001330962.1	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ash-2 ( NM_064154.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496555.1 Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Price & Availability↑

CloneID	OCa338024
Clone ID Related Accession (Same CDS sequence)	NM_064154.1 , NM_064154.2
Accession Version	NM_064154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Set1/Ash2 histone methyltransferase complex subunit ash-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AGCAGCCGAC 
TATTCATCAA CTGCAGCTCC AAATGGTGTT CAGATTGATA TACCATTCAG TAAGGACAAT 
TATCGATATT ATCTCACTGA AGTCGATCCA AATGTACCGG AAGACCCAGC ATGGAATCAA 
AATCAATCAT CAGCGTATGT GATACCATCA TTTCATTATC GAGAACTATT AAATCCAACA 
GTAAATGTAT CATCAAATGA TCGTGCATTT CAATTATCGA TTAATGGAAA TTCAATAACT 
GGATTTGAAG GATATTCAAT GGCACGTGCA AGTCATGGTG TATCTAAAGG AACATGGTAT 
TTCGAAGTTA ATTTTGATGA TCAACCTGAT GATTCACATA TTCGTATTGG ATGGAGTCAG 
TCTTATGCAT CCCTGCAAGC ATGTGTTGGA TACAACAAAT TCTCATATGG ATGGCGATCG 
AAACACGGAA CGAAATTTCA TGAGGCAAAA GGAAAAAAGT ATCATTTTGG AGGTTTTAAG 
CAAGGCGACG TTCTCGGCTG TCTAATCCAT CTTCCAGTGG ACAAAAAACT GCAGATTCCG 
GCAAATTTGC CATCGGAAAA GTATCTTCCA GTATCACACA AAGGCTTCAA TTTGATATCA 
TTTAAAGCGA ATTATTTCTT TGAAGTCCAA GAGGAGTCCG CAGATATTGC AAAGACTCTG 
GTGGAAATGC CTGGAAGCTA TATCGAGTTC TTCCACAATG GAAAATCGTG CGGAAAAGCA 
TATGAGAATA TCTACGCCGG CGCCTATTAC CCGTCGATTT CCATATTTAA ATCTGCCACA 
GCCACCATGA ATCTCGGCCC TAAATTCCGT AATCTGCCAC GTGGTGCCAC CGGAATCCAC 
GCGAGAGCCG ACGAGCAGCA ACACGAACAA ACTCTTTCGG ATATGCTGTA TTTGGTGTCA 
AAAGAAGTGA ATTTGGACCA TCCGCCGCGT GTAAAACGAG AGGACGATGA TGACGTGAAG 
GATATCAAGA AAGAAATTAA ACAAGAAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496555.1
CDS	1..1713
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHELNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_064154.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064154.1

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AGCAGCCGAC 
TATTCATCAA CTGCAGCTCC AAATGGTGTT CAGATTGATA TACCATTCAG TAAGGACAAT 
TATCGATATT ATCTCACTGA AGTCGATCCA AATGTACCGG AAGACCCAGC ATGGAATCAA 
AATCAATCAT CAGCGTATGT GATACCATCA TTTCATTATC GAGAACTATT AAATCCAACA 
GTAAATGTAT CATCAAATGA TCGTGCATTT CAATTATCGA TTAATGGAAA TTCAATAACT 
GGATTTGAAG GATATTCAAT GGCACGTGCA AGTCATGGTG TATCTAAAGG AACATGGTAT 
TTCGAAGTTA ATTTTGATGA TCAACCTGAT GATTCACATA TTCGTATTGG ATGGAGTCAG 
TCTTATGCAT CCCTGCAAGC ATGTGTTGGA TACAACAAAT TCTCATATGG ATGGCGATCG 
AAACACGGAA CGAAATTTCA TGAGGCAAAA GGAAAAAAGT ATCATTTTGG AGGTTTTAAG 
CAAGGCGACG TTCTCGGCTG TCTAATCCAT CTTCCAGTGG ACAAAAAACT GCAGATTCCG 
GCAAATTTGC CATCGGAAAA GTATCTTCCA GTATCACACA AAGGCTTCAA TTTGATATCA 
TTTAAAGCGA ATTATTTCTT TGAAGTCCAA GAGGAGTCCG CAGATATTGC AAAGACTCTG 
GTGGAAATGC CTGGAAGCTA TATCGAGTTC TTCCACAATG GAAAATCGTG CGGAAAAGCA 
TATGAGAATA TCTACGCCGG CGCCTATTAC CCGTCGATTT CCATATTTAA ATCTGCCACA 
GCCACCATGA ATCTCGGCCC TAAATTCCGT AATCTGCCAC GTGGTGCCAC CGGAATCCAC 
GCGAGAGCCG ACGAGCAGCA ACACGAACAA ACTCTTTCGG ATATGCTGTA TTTGGTGTCA 
AAAGAAGTGA ATTTGGACCA TCCGCCGCGT GTAAAACGAG AGGACGATGA TGACGTGAAG 
GATATCAAGA AAGAAATTAA ACAAGAAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_064154.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496555.1 Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), mRNA.

Price & Availability↑

CloneID	OCa338024
Clone ID Related Accession (Same CDS sequence)	NM_064154.1 , NM_064154.2
Accession Version	NM_064154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Set1/Ash2 histone methyltransferase complex subunit ash-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064154.1.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AGCAGCCGAC 
TATTCATCAA CTGCAGCTCC AAATGGTGTT CAGATTGATA TACCATTCAG TAAGGACAAT 
TATCGATATT ATCTCACTGA AGTCGATCCA AATGTACCGG AAGACCCAGC ATGGAATCAA 
AATCAATCAT CAGCGTATGT GATACCATCA TTTCATTATC GAGAACTATT AAATCCAACA 
GTAAATGTAT CATCAAATGA TCGTGCATTT CAATTATCGA TTAATGGAAA TTCAATAACT 
GGATTTGAAG GATATTCAAT GGCACGTGCA AGTCATGGTG TATCTAAAGG AACATGGTAT 
TTCGAAGTTA ATTTTGATGA TCAACCTGAT GATTCACATA TTCGTATTGG ATGGAGTCAG 
TCTTATGCAT CCCTGCAAGC ATGTGTTGGA TACAACAAAT TCTCATATGG ATGGCGATCG 
AAACACGGAA CGAAATTTCA TGAGGCAAAA GGAAAAAAGT ATCATTTTGG AGGTTTTAAG 
CAAGGCGACG TTCTCGGCTG TCTAATCCAT CTTCCAGTGG ACAAAAAACT GCAGATTCCG 
GCAAATTTGC CATCGGAAAA GTATCTTCCA GTATCACACA AAGGCTTCAA TTTGATATCA 
TTTAAAGCGA ATTATTTCTT TGAAGTCCAA GAGGAGTCCG CAGATATTGC AAAGACTCTG 
GTGGAAATGC CTGGAAGCTA TATCGAGTTC TTCCACAATG GAAAATCGTG CGGAAAAGCA 
TATGAGAATA TCTACGCCGG CGCCTATTAC CCGTCGATTT CCATATTTAA ATCTGCCACA 
GCCACCATGA ATCTCGGCCC TAAATTCCGT AATCTGCCAC GTGGTGCCAC CGGAATCCAC 
GCGAGAGCCG ACGAGCAGCA ACACGAACAA ACTCTTTCGG ATATGCTGTA TTTGGTGTCA 
AAAGAAGTGA ATTTGGACCA TCCGCCGCGT GTAAAACGAG AGGACGATGA TGACGTGAAG 
GATATCAAGA AAGAAATTAA ACAAGAAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496555.1
CDS	12..1724
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHELNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_064154.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064154.2

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AGCAGCCGAC 
TATTCATCAA CTGCAGCTCC AAATGGTGTT CAGATTGATA TACCATTCAG TAAGGACAAT 
TATCGATATT ATCTCACTGA AGTCGATCCA AATGTACCGG AAGACCCAGC ATGGAATCAA 
AATCAATCAT CAGCGTATGT GATACCATCA TTTCATTATC GAGAACTATT AAATCCAACA 
GTAAATGTAT CATCAAATGA TCGTGCATTT CAATTATCGA TTAATGGAAA TTCAATAACT 
GGATTTGAAG GATATTCAAT GGCACGTGCA AGTCATGGTG TATCTAAAGG AACATGGTAT 
TTCGAAGTTA ATTTTGATGA TCAACCTGAT GATTCACATA TTCGTATTGG ATGGAGTCAG 
TCTTATGCAT CCCTGCAAGC ATGTGTTGGA TACAACAAAT TCTCATATGG ATGGCGATCG 
AAACACGGAA CGAAATTTCA TGAGGCAAAA GGAAAAAAGT ATCATTTTGG AGGTTTTAAG 
CAAGGCGACG TTCTCGGCTG TCTAATCCAT CTTCCAGTGG ACAAAAAACT GCAGATTCCG 
GCAAATTTGC CATCGGAAAA GTATCTTCCA GTATCACACA AAGGCTTCAA TTTGATATCA 
TTTAAAGCGA ATTATTTCTT TGAAGTCCAA GAGGAGTCCG CAGATATTGC AAAGACTCTG 
GTGGAAATGC CTGGAAGCTA TATCGAGTTC TTCCACAATG GAAAATCGTG CGGAAAAGCA 
TATGAGAATA TCTACGCCGG CGCCTATTAC CCGTCGATTT CCATATTTAA ATCTGCCACA 
GCCACCATGA ATCTCGGCCC TAAATTCCGT AATCTGCCAC GTGGTGCCAC CGGAATCCAC 
GCGAGAGCCG ACGAGCAGCA ACACGAACAA ACTCTTTCGG ATATGCTGTA TTTGGTGTCA 
AAAGAAGTGA ATTTGGACCA TCCGCCGCGT GTAAAACGAG AGGACGATGA TGACGTGAAG 
GATATCAAGA AAGAAATTAA ACAAGAAATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_064152.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496553.1 Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Price & Availability↑

CloneID	OCa338032
Clone ID Related Accession (Same CDS sequence)	NM_064152.3 , NM_001373799.1
Accession Version	NM_064152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Set1/Ash2 histone methyltransferase complex subunit ash-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064152.2. COMPLETENESS: incomplete on both ends.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAGCA 
GCCGACTATT CATCAACTGC AGCTCCAAAT GGTGTTCAGA TTGATATACC ATTCAGTAAG 
GACAATTATC GATATTATCT CACTGAAGTC GATCCAAATG TACCGGAAGA CCCAGCATGG 
AATCAAAATC AATCATCAGC GTATGTGATA CCATCATTTC ATTATCGAGA ACTATTAAAT 
CCAACAGTAA ATGTATCATC AAATGATCGT GCATTTCAAT TATCGATTAA TGGAAATTCA 
ATAACTGGAT TTGAAGGATA TTCAATGGCA CGTGCAAGTC ATGGTGTATC TAAAGGAACA 
TGGTATTTCG AAGTTAATTT TGATGATCAA CCTGATGATT CACATATTCG TATTGGATGG 
AGTCAGTCTT ATGCATCCCT GCAAGCATGT GTTGGATACA ACAAATTCTC ATATGGATGG 
CGATCGAAAC ACGGAACGAA ATTTCATGAG GCAAAAGGAA AAAAGTATCA TTTTGGAGGT 
TTTAAGCAAG GCGACGTTCT CGGCTGTCTA ATCCATCTTC CAGTGGACAA AAAACTGCAG 
ATTCCGGCAA ATTTGCCATC GGAAAAGTAT CTTCCAGTAT CACACAAAGG CTTCAATTTG 
ATATCATTTA AAGCGAATTA TTTCTTTGAA GTCCAAGAGG AGTCCGCAGA TATTGCAAAG 
ACTCTGGTGG AAATGCCTGG AAGCTATATC GAGTTCTTCC ACAATGGAAA ATCGTGCGGA 
AAAGCATATG AGAATATCTA CGCCGGCGCC TATTACCCGT CGATTTCCAT ATTTAAATCT 
GCCACAGCCA CCATGAATCT CGGCCCTAAA TTCCGTAATC TGCCACGTGG TGCCACCGGA 
ATCCACGCGA GAGCCGACGA GCAGCAACAC GAACAAACTC TTTCGGATAT GCTGTATTTG 
GTGTCAAAAG AAGTGAATTT GGACCATCCG CCGCGTGTAA AACGAGAGGA CGATGATGAC 
GTGAAGGATA TCAAGAAAGA AATTAAACAA GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496553.1
CDS	1..1719
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHEFSRNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_064152.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064152.3

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAGCA 
GCCGACTATT CATCAACTGC AGCTCCAAAT GGTGTTCAGA TTGATATACC ATTCAGTAAG 
GACAATTATC GATATTATCT CACTGAAGTC GATCCAAATG TACCGGAAGA CCCAGCATGG 
AATCAAAATC AATCATCAGC GTATGTGATA CCATCATTTC ATTATCGAGA ACTATTAAAT 
CCAACAGTAA ATGTATCATC AAATGATCGT GCATTTCAAT TATCGATTAA TGGAAATTCA 
ATAACTGGAT TTGAAGGATA TTCAATGGCA CGTGCAAGTC ATGGTGTATC TAAAGGAACA 
TGGTATTTCG AAGTTAATTT TGATGATCAA CCTGATGATT CACATATTCG TATTGGATGG 
AGTCAGTCTT ATGCATCCCT GCAAGCATGT GTTGGATACA ACAAATTCTC ATATGGATGG 
CGATCGAAAC ACGGAACGAA ATTTCATGAG GCAAAAGGAA AAAAGTATCA TTTTGGAGGT 
TTTAAGCAAG GCGACGTTCT CGGCTGTCTA ATCCATCTTC CAGTGGACAA AAAACTGCAG 
ATTCCGGCAA ATTTGCCATC GGAAAAGTAT CTTCCAGTAT CACACAAAGG CTTCAATTTG 
ATATCATTTA AAGCGAATTA TTTCTTTGAA GTCCAAGAGG AGTCCGCAGA TATTGCAAAG 
ACTCTGGTGG AAATGCCTGG AAGCTATATC GAGTTCTTCC ACAATGGAAA ATCGTGCGGA 
AAAGCATATG AGAATATCTA CGCCGGCGCC TATTACCCGT CGATTTCCAT ATTTAAATCT 
GCCACAGCCA CCATGAATCT CGGCCCTAAA TTCCGTAATC TGCCACGTGG TGCCACCGGA 
ATCCACGCGA GAGCCGACGA GCAGCAACAC GAACAAACTC TTTCGGATAT GCTGTATTTG 
GTGTCAAAAG AAGTGAATTT GGACCATCCG CCGCGTGTAA AACGAGAGGA CGATGATGAC 
GTGAAGGATA TCAAGAAAGA AATTAAACAA GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_001373799.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001359515.1 Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

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CloneID	OCa338032
Clone ID Related Accession (Same CDS sequence)	NM_064152.3 , NM_001373799.1
Accession Version	NM_001373799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	B30.2/SPRY domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280).

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAGCA 
GCCGACTATT CATCAACTGC AGCTCCAAAT GGTGTTCAGA TTGATATACC ATTCAGTAAG 
GACAATTATC GATATTATCT CACTGAAGTC GATCCAAATG TACCGGAAGA CCCAGCATGG 
AATCAAAATC AATCATCAGC GTATGTGATA CCATCATTTC ATTATCGAGA ACTATTAAAT 
CCAACAGTAA ATGTATCATC AAATGATCGT GCATTTCAAT TATCGATTAA TGGAAATTCA 
ATAACTGGAT TTGAAGGATA TTCAATGGCA CGTGCAAGTC ATGGTGTATC TAAAGGAACA 
TGGTATTTCG AAGTTAATTT TGATGATCAA CCTGATGATT CACATATTCG TATTGGATGG 
AGTCAGTCTT ATGCATCCCT GCAAGCATGT GTTGGATACA ACAAATTCTC ATATGGATGG 
CGATCGAAAC ACGGAACGAA ATTTCATGAG GCAAAAGGAA AAAAGTATCA TTTTGGAGGT 
TTTAAGCAAG GCGACGTTCT CGGCTGTCTA ATCCATCTTC CAGTGGACAA AAAACTGCAG 
ATTCCGGCAA ATTTGCCATC GGAAAAGTAT CTTCCAGTAT CACACAAAGG CTTCAATTTG 
ATATCATTTA AAGCGAATTA TTTCTTTGAA GTCCAAGAGG AGTCCGCAGA TATTGCAAAG 
ACTCTGGTGG AAATGCCTGG AAGCTATATC GAGTTCTTCC ACAATGGAAA ATCGTGCGGA 
AAAGCATATG AGAATATCTA CGCCGGCGCC TATTACCCGT CGATTTCCAT ATTTAAATCT 
GCCACAGCCA CCATGAATCT CGGCCCTAAA TTCCGTAATC TGCCACGTGG TGCCACCGGA 
ATCCACGCGA GAGCCGACGA GCAGCAACAC GAACAAACTC TTTCGGATAT GCTGTATTTG 
GTGTCAAAAG AAGTGAATTT GGACCATCCG CCGCGTGTAA AACGAGAGGA CGATGATGAC 
GTGAAGGATA TCAAGAAAGA AATTAAACAA GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359515.1
CDS	50..1768
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHEFSRNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_001373799.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373799.1

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAGCA 
GCCGACTATT CATCAACTGC AGCTCCAAAT GGTGTTCAGA TTGATATACC ATTCAGTAAG 
GACAATTATC GATATTATCT CACTGAAGTC GATCCAAATG TACCGGAAGA CCCAGCATGG 
AATCAAAATC AATCATCAGC GTATGTGATA CCATCATTTC ATTATCGAGA ACTATTAAAT 
CCAACAGTAA ATGTATCATC AAATGATCGT GCATTTCAAT TATCGATTAA TGGAAATTCA 
ATAACTGGAT TTGAAGGATA TTCAATGGCA CGTGCAAGTC ATGGTGTATC TAAAGGAACA 
TGGTATTTCG AAGTTAATTT TGATGATCAA CCTGATGATT CACATATTCG TATTGGATGG 
AGTCAGTCTT ATGCATCCCT GCAAGCATGT GTTGGATACA ACAAATTCTC ATATGGATGG 
CGATCGAAAC ACGGAACGAA ATTTCATGAG GCAAAAGGAA AAAAGTATCA TTTTGGAGGT 
TTTAAGCAAG GCGACGTTCT CGGCTGTCTA ATCCATCTTC CAGTGGACAA AAAACTGCAG 
ATTCCGGCAA ATTTGCCATC GGAAAAGTAT CTTCCAGTAT CACACAAAGG CTTCAATTTG 
ATATCATTTA AAGCGAATTA TTTCTTTGAA GTCCAAGAGG AGTCCGCAGA TATTGCAAAG 
ACTCTGGTGG AAATGCCTGG AAGCTATATC GAGTTCTTCC ACAATGGAAA ATCGTGCGGA 
AAAGCATATG AGAATATCTA CGCCGGCGCC TATTACCCGT CGATTTCCAT ATTTAAATCT 
GCCACAGCCA CCATGAATCT CGGCCCTAAA TTCCGTAATC TGCCACGTGG TGCCACCGGA 
ATCCACGCGA GAGCCGACGA GCAGCAACAC GAACAAACTC TTTCGGATAT GCTGTATTTG 
GTGTCAAAAG AAGTGAATTT GGACCATCCG CCGCGTGTAA AACGAGAGGA CGATGATGAC 
GTGAAGGATA TCAAGAAAGA AATTAAACAA GAAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_001373798.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360415.1 Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

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CloneID	OCa338166
Clone ID Related Accession (Same CDS sequence)	NM_001373798.1 , NM_001330961.1
Accession Version	NM_001373798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	B30.2/SPRY domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280).

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAACT 
TCCAGAGCAG CCGACTATTC ATCAACTGCA GCTCCAAATG GTGTTCAGAT TGATATACCA 
TTCAGTAAGG ACAATTATCG ATATTATCTC ACTGAAGTCG ATCCAAATGT ACCGGAAGAC 
CCAGCATGGA ATCAAAATCA ATCATCAGCG TATGTGATAC CATCATTTCA TTATCGAGAA 
CTATTAAATC CAACAGTAAA TGTATCATCA AATGATCGTG CATTTCAATT ATCGATTAAT 
GGAAATTCAA TAACTGGATT TGAAGGATAT TCAATGGCAC GTGCAAGTCA TGGTGTATCT 
AAAGGAACAT GGTATTTCGA AGTTAATTTT GATGATCAAC CTGATGATTC ACATATTCGT 
ATTGGATGGA GTCAGTCTTA TGCATCCCTG CAAGCATGTG TTGGATACAA CAAATTCTCA 
TATGGATGGC GATCGAAACA CGGAACGAAA TTTCATGAGG CAAAAGGAAA AAAGTATCAT 
TTTGGAGGTT TTAAGCAAGG CGACGTTCTC GGCTGTCTAA TCCATCTTCC AGTGGACAAA 
AAACTGCAGA TTCCGGCAAA TTTGCCATCG GAAAAGTATC TTCCAGTATC ACACAAAGGC 
TTCAATTTGA TATCATTTAA AGCGAATTAT TTCTTTGAAG TCCAAGAGGA GTCCGCAGAT 
ATTGCAAAGA CTCTGGTGGA AATGCCTGGA AGCTATATCG AGTTCTTCCA CAATGGAAAA 
TCGTGCGGAA AAGCATATGA GAATATCTAC GCCGGCGCCT ATTACCCGTC GATTTCCATA 
TTTAAATCTG CCACAGCCAC CATGAATCTC GGCCCTAAAT TCCGTAATCT GCCACGTGGT 
GCCACCGGAA TCCACGCGAG AGCCGACGAG CAGCAACACG AACAAACTCT TTCGGATATG 
CTGTATTTGG TGTCAAAAGA AGTGAATTTG GACCATCCGC CGCGTGTAAA ACGAGAGGAC 
GATGATGACG TGAAGGATAT CAAGAAAGAA ATTAAACAAG AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360415.1
CDS	50..1777
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHEFSRNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLTSRAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_001373798.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373798.1

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAACT 
TCCAGAGCAG CCGACTATTC ATCAACTGCA GCTCCAAATG GTGTTCAGAT TGATATACCA 
TTCAGTAAGG ACAATTATCG ATATTATCTC ACTGAAGTCG ATCCAAATGT ACCGGAAGAC 
CCAGCATGGA ATCAAAATCA ATCATCAGCG TATGTGATAC CATCATTTCA TTATCGAGAA 
CTATTAAATC CAACAGTAAA TGTATCATCA AATGATCGTG CATTTCAATT ATCGATTAAT 
GGAAATTCAA TAACTGGATT TGAAGGATAT TCAATGGCAC GTGCAAGTCA TGGTGTATCT 
AAAGGAACAT GGTATTTCGA AGTTAATTTT GATGATCAAC CTGATGATTC ACATATTCGT 
ATTGGATGGA GTCAGTCTTA TGCATCCCTG CAAGCATGTG TTGGATACAA CAAATTCTCA 
TATGGATGGC GATCGAAACA CGGAACGAAA TTTCATGAGG CAAAAGGAAA AAAGTATCAT 
TTTGGAGGTT TTAAGCAAGG CGACGTTCTC GGCTGTCTAA TCCATCTTCC AGTGGACAAA 
AAACTGCAGA TTCCGGCAAA TTTGCCATCG GAAAAGTATC TTCCAGTATC ACACAAAGGC 
TTCAATTTGA TATCATTTAA AGCGAATTAT TTCTTTGAAG TCCAAGAGGA GTCCGCAGAT 
ATTGCAAAGA CTCTGGTGGA AATGCCTGGA AGCTATATCG AGTTCTTCCA CAATGGAAAA 
TCGTGCGGAA AAGCATATGA GAATATCTAC GCCGGCGCCT ATTACCCGTC GATTTCCATA 
TTTAAATCTG CCACAGCCAC CATGAATCTC GGCCCTAAAT TCCGTAATCT GCCACGTGGT 
GCCACCGGAA TCCACGCGAG AGCCGACGAG CAGCAACACG AACAAACTCT TTCGGATATG 
CTGTATTTGG TGTCAAAAGA AGTGAATTTG GACCATCCGC CGCGTGTAAA ACGAGAGGAC 
GATGATGACG TGAAGGATAT CAAGAAAGAA ATTAAACAAG AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_001330961.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317780.1 Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Price & Availability↑

CloneID	OCa338166
Clone ID Related Accession (Same CDS sequence)	NM_001373798.1 , NM_001330961.1
Accession Version	NM_001330961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Set1/Ash2 histone methyltransferase complex subunit ash-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAACT 
TCCAGAGCAG CCGACTATTC ATCAACTGCA GCTCCAAATG GTGTTCAGAT TGATATACCA 
TTCAGTAAGG ACAATTATCG ATATTATCTC ACTGAAGTCG ATCCAAATGT ACCGGAAGAC 
CCAGCATGGA ATCAAAATCA ATCATCAGCG TATGTGATAC CATCATTTCA TTATCGAGAA 
CTATTAAATC CAACAGTAAA TGTATCATCA AATGATCGTG CATTTCAATT ATCGATTAAT 
GGAAATTCAA TAACTGGATT TGAAGGATAT TCAATGGCAC GTGCAAGTCA TGGTGTATCT 
AAAGGAACAT GGTATTTCGA AGTTAATTTT GATGATCAAC CTGATGATTC ACATATTCGT 
ATTGGATGGA GTCAGTCTTA TGCATCCCTG CAAGCATGTG TTGGATACAA CAAATTCTCA 
TATGGATGGC GATCGAAACA CGGAACGAAA TTTCATGAGG CAAAAGGAAA AAAGTATCAT 
TTTGGAGGTT TTAAGCAAGG CGACGTTCTC GGCTGTCTAA TCCATCTTCC AGTGGACAAA 
AAACTGCAGA TTCCGGCAAA TTTGCCATCG GAAAAGTATC TTCCAGTATC ACACAAAGGC 
TTCAATTTGA TATCATTTAA AGCGAATTAT TTCTTTGAAG TCCAAGAGGA GTCCGCAGAT 
ATTGCAAAGA CTCTGGTGGA AATGCCTGGA AGCTATATCG AGTTCTTCCA CAATGGAAAA 
TCGTGCGGAA AAGCATATGA GAATATCTAC GCCGGCGCCT ATTACCCGTC GATTTCCATA 
TTTAAATCTG CCACAGCCAC CATGAATCTC GGCCCTAAAT TCCGTAATCT GCCACGTGGT 
GCCACCGGAA TCCACGCGAG AGCCGACGAG CAGCAACACG AACAAACTCT TTCGGATATG 
CTGTATTTGG TGTCAAAAGA AGTGAATTTG GACCATCCGC CGCGTGTAAA ACGAGAGGAC 
GATGATGACG TGAAGGATAT CAAGAAAGAA ATTAAACAAG AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317780.1
CDS	1..1728
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHEFSRNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLTSRAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_001330961.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330961.1

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTTTCCAG AAACAGCAAC 
GGCGTGCCTT TCATGATTTG CTACACGTTC ACCTGCAAAC AATGCCGGCC GACGGCCGAA 
GATTGGAAGG CTAAAAAGGC GGATTTAGTG CAAATGTGTG TTACAGTGCT CGCCACACTG 
TCCGCCGAGC GGCTTAAAGC CGACGGAAAG CTCTCGGCTG AGCATGTTCC AGAGGATTTT 
ACATATTTAA GTCTGAAAGA TGAAATTGTG CCATATATGA ATGAGAATTG GTATATGCTC 
ACTGCAATCA AGCAGAAAAA GGAATGGCAT CAGAATTTGG CGCCAACTTT GCTCAAAGAG 
AAGAATATTT TCGTGCAACA CAACGACGAT GATGATCTAT TTGCATTGGC AGAAAAGAAT 
TTATCACTTC TAGGACCACT ACACGAGGCA GTTAAACTAA TCGGAAAACG TCCAATCGAA 
AGGGAAAACC GTGAGCCACG TCATATTGAG CTTCCACCGA TTGAGGGACC GAAAACTCGT 
GGAGCATCGA AACGCCGGCA CGCCGAGGCT CCGGTGACCG GAAAAAAGCA GAAATTAACT 
TCCAGAGCAG CCGACTATTC ATCAACTGCA GCTCCAAATG GTGTTCAGAT TGATATACCA 
TTCAGTAAGG ACAATTATCG ATATTATCTC ACTGAAGTCG ATCCAAATGT ACCGGAAGAC 
CCAGCATGGA ATCAAAATCA ATCATCAGCG TATGTGATAC CATCATTTCA TTATCGAGAA 
CTATTAAATC CAACAGTAAA TGTATCATCA AATGATCGTG CATTTCAATT ATCGATTAAT 
GGAAATTCAA TAACTGGATT TGAAGGATAT TCAATGGCAC GTGCAAGTCA TGGTGTATCT 
AAAGGAACAT GGTATTTCGA AGTTAATTTT GATGATCAAC CTGATGATTC ACATATTCGT 
ATTGGATGGA GTCAGTCTTA TGCATCCCTG CAAGCATGTG TTGGATACAA CAAATTCTCA 
TATGGATGGC GATCGAAACA CGGAACGAAA TTTCATGAGG CAAAAGGAAA AAAGTATCAT 
TTTGGAGGTT TTAAGCAAGG CGACGTTCTC GGCTGTCTAA TCCATCTTCC AGTGGACAAA 
AAACTGCAGA TTCCGGCAAA TTTGCCATCG GAAAAGTATC TTCCAGTATC ACACAAAGGC 
TTCAATTTGA TATCATTTAA AGCGAATTAT TTCTTTGAAG TCCAAGAGGA GTCCGCAGAT 
ATTGCAAAGA CTCTGGTGGA AATGCCTGGA AGCTATATCG AGTTCTTCCA CAATGGAAAA 
TCGTGCGGAA AAGCATATGA GAATATCTAC GCCGGCGCCT ATTACCCGTC GATTTCCATA 
TTTAAATCTG CCACAGCCAC CATGAATCTC GGCCCTAAAT TCCGTAATCT GCCACGTGGT 
GCCACCGGAA TCCACGCGAG AGCCGACGAG CAGCAACACG AACAAACTCT TTCGGATATG 
CTGTATTTGG TGTCAAAAGA AGTGAATTTG GACCATCCGC CGCGTGTAAA ACGAGAGGAC 
GATGATGACG TGAAGGATAT CAAGAAAGAA ATTAAACAAG AAATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_001330962.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317781.1 Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

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CloneID	OCa338167
Clone ID Related Accession (Same CDS sequence)	NM_001330962.2 , NM_001330962.1
Accession Version	NM_001330962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	B30.2/SPRY domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001330962.1.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AACTTCCAGA 
GCAGCCGACT ATTCATCAAC TGCAGCTCCA AATGGTGTTC AGATTGATAT ACCATTCAGT 
AAGGACAATT ATCGATATTA TCTCACTGAA GTCGATCCAA ATGTACCGGA AGACCCAGCA 
TGGAATCAAA ATCAATCATC AGCGTATGTG ATACCATCAT TTCATTATCG AGAACTATTA 
AATCCAACAG TAAATGTATC ATCAAATGAT CGTGCATTTC AATTATCGAT TAATGGAAAT 
TCAATAACTG GATTTGAAGG ATATTCAATG GCACGTGCAA GTCATGGTGT ATCTAAAGGA 
ACATGGTATT TCGAAGTTAA TTTTGATGAT CAACCTGATG ATTCACATAT TCGTATTGGA 
TGGAGTCAGT CTTATGCATC CCTGCAAGCA TGTGTTGGAT ACAACAAATT CTCATATGGA 
TGGCGATCGA AACACGGAAC GAAATTTCAT GAGGCAAAAG GAAAAAAGTA TCATTTTGGA 
GGTTTTAAGC AAGGCGACGT TCTCGGCTGT CTAATCCATC TTCCAGTGGA CAAAAAACTG 
CAGATTCCGG CAAATTTGCC ATCGGAAAAG TATCTTCCAG TATCACACAA AGGCTTCAAT 
TTGATATCAT TTAAAGCGAA TTATTTCTTT GAAGTCCAAG AGGAGTCCGC AGATATTGCA 
AAGACTCTGG TGGAAATGCC TGGAAGCTAT ATCGAGTTCT TCCACAATGG AAAATCGTGC 
GGAAAAGCAT ATGAGAATAT CTACGCCGGC GCCTATTACC CGTCGATTTC CATATTTAAA 
TCTGCCACAG CCACCATGAA TCTCGGCCCT AAATTCCGTA ATCTGCCACG TGGTGCCACC 
GGAATCCACG CGAGAGCCGA CGAGCAGCAA CACGAACAAA CTCTTTCGGA TATGCTGTAT 
TTGGTGTCAA AAGAAGTGAA TTTGGACCAT CCGCCGCGTG TAAAACGAGA GGACGATGAT 
GACGTGAAGG ATATCAAGAA AGAAATTAAA CAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317781.1
CDS	11..1732
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHELNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLTSRAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_001330962.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans B30.2/SPRY domain-containing protein (ash-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330962.2

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AACTTCCAGA 
GCAGCCGACT ATTCATCAAC TGCAGCTCCA AATGGTGTTC AGATTGATAT ACCATTCAGT 
AAGGACAATT ATCGATATTA TCTCACTGAA GTCGATCCAA ATGTACCGGA AGACCCAGCA 
TGGAATCAAA ATCAATCATC AGCGTATGTG ATACCATCAT TTCATTATCG AGAACTATTA 
AATCCAACAG TAAATGTATC ATCAAATGAT CGTGCATTTC AATTATCGAT TAATGGAAAT 
TCAATAACTG GATTTGAAGG ATATTCAATG GCACGTGCAA GTCATGGTGT ATCTAAAGGA 
ACATGGTATT TCGAAGTTAA TTTTGATGAT CAACCTGATG ATTCACATAT TCGTATTGGA 
TGGAGTCAGT CTTATGCATC CCTGCAAGCA TGTGTTGGAT ACAACAAATT CTCATATGGA 
TGGCGATCGA AACACGGAAC GAAATTTCAT GAGGCAAAAG GAAAAAAGTA TCATTTTGGA 
GGTTTTAAGC AAGGCGACGT TCTCGGCTGT CTAATCCATC TTCCAGTGGA CAAAAAACTG 
CAGATTCCGG CAAATTTGCC ATCGGAAAAG TATCTTCCAG TATCACACAA AGGCTTCAAT 
TTGATATCAT TTAAAGCGAA TTATTTCTTT GAAGTCCAAG AGGAGTCCGC AGATATTGCA 
AAGACTCTGG TGGAAATGCC TGGAAGCTAT ATCGAGTTCT TCCACAATGG AAAATCGTGC 
GGAAAAGCAT ATGAGAATAT CTACGCCGGC GCCTATTACC CGTCGATTTC CATATTTAAA 
TCTGCCACAG CCACCATGAA TCTCGGCCCT AAATTCCGTA ATCTGCCACG TGGTGCCACC 
GGAATCCACG CGAGAGCCGA CGAGCAGCAA CACGAACAAA CTCTTTCGGA TATGCTGTAT 
TTGGTGTCAA AAGAAGTGAA TTTGGACCAT CCGCCGCGTG TAAAACGAGA GGACGATGAT 
GACGTGAAGG ATATCAAGAA AGAAATTAAA CAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash-2 ( NM_001330962.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317781.1 Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

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CloneID	OCa338167
Clone ID Related Accession (Same CDS sequence)	NM_001330962.2 , NM_001330962.1
Accession Version	NM_001330962.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Set1/Ash2 histone methyltransferase complex subunit ash-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AACTTCCAGA 
GCAGCCGACT ATTCATCAAC TGCAGCTCCA AATGGTGTTC AGATTGATAT ACCATTCAGT 
AAGGACAATT ATCGATATTA TCTCACTGAA GTCGATCCAA ATGTACCGGA AGACCCAGCA 
TGGAATCAAA ATCAATCATC AGCGTATGTG ATACCATCAT TTCATTATCG AGAACTATTA 
AATCCAACAG TAAATGTATC ATCAAATGAT CGTGCATTTC AATTATCGAT TAATGGAAAT 
TCAATAACTG GATTTGAAGG ATATTCAATG GCACGTGCAA GTCATGGTGT ATCTAAAGGA 
ACATGGTATT TCGAAGTTAA TTTTGATGAT CAACCTGATG ATTCACATAT TCGTATTGGA 
TGGAGTCAGT CTTATGCATC CCTGCAAGCA TGTGTTGGAT ACAACAAATT CTCATATGGA 
TGGCGATCGA AACACGGAAC GAAATTTCAT GAGGCAAAAG GAAAAAAGTA TCATTTTGGA 
GGTTTTAAGC AAGGCGACGT TCTCGGCTGT CTAATCCATC TTCCAGTGGA CAAAAAACTG 
CAGATTCCGG CAAATTTGCC ATCGGAAAAG TATCTTCCAG TATCACACAA AGGCTTCAAT 
TTGATATCAT TTAAAGCGAA TTATTTCTTT GAAGTCCAAG AGGAGTCCGC AGATATTGCA 
AAGACTCTGG TGGAAATGCC TGGAAGCTAT ATCGAGTTCT TCCACAATGG AAAATCGTGC 
GGAAAAGCAT ATGAGAATAT CTACGCCGGC GCCTATTACC CGTCGATTTC CATATTTAAA 
TCTGCCACAG CCACCATGAA TCTCGGCCCT AAATTCCGTA ATCTGCCACG TGGTGCCACC 
GGAATCCACG CGAGAGCCGA CGAGCAGCAA CACGAACAAA CTCTTTCGGA TATGCTGTAT 
TTGGTGTCAA AAGAAGTGAA TTTGGACCAT CCGCCGCGTG TAAAACGAGA GGACGATGAT 
GACGTGAAGG ATATCAAGAA AGAAATTAAA CAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317781.1
CDS	1..1722
Translation	MRSSKGGRGRQAAPKTAPTTVCYCDGKRELGSVEVVCSTCLKWFHGRCLKEFHELNSNGVPFMICYTFTCKQCRPTAEDWKAKKADLVQMCVTVLATLSAERLKADGKLSAEHVPEDFTYLSLKDEIVPYMNENWYMLTAIKQKKEWHQNLAPTLLKEKNIFVQHNDDDDLFALAEKNLSLLGPLHEAVKLIGKRPIERENREPRHIELPPIEGPKTRGASKRRHAEAPVTGKKQKLTSRAADYSSTAAPNGVQIDIPFSKDNYRYYLTEVDPNVPEDPAWNQNQSSAYVIPSFHYRELLNPTVNVSSNDRAFQLSINGNSITGFEGYSMARASHGVSKGTWYFEVNFDDQPDDSHIRIGWSQSYASLQACVGYNKFSYGWRSKHGTKFHEAKGKKYHFGGFKQGDVLGCLIHLPVDKKLQIPANLPSEKYLPVSHKGFNLISFKANYFFEVQEESADIAKTLVEMPGSYIEFFHNGKSCGKAYENIYAGAYYPSISIFKSATATMNLGPKFRNLPRGATGIHARADEQQHEQTLSDMLYLVSKEVNLDHPPRVKREDDDDVKDIKKEIKQEI

Target ORF information:

RefSeq Version	NM_001330962.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Set1/Ash2 histone methyltransferase complex subunit ash-2 (ash-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330962.1

ORF Insert Sequence:

ATGAGAAGCT CAAAAGGAGG TCGGGGACGT CAAGCGGCGC CAAAAACCGC TCCAACCACT 
GTCTGCTACT GCGACGGAAA GCGAGAGCTT GGCTCAGTGG AAGTAGTGTG CTCGACGTGT 
CTCAAATGGT TCCACGGACG ATGTCTAAAA GAGTTTCACG AGTTAAACAG CAACGGCGTG 
CCTTTCATGA TTTGCTACAC GTTCACCTGC AAACAATGCC GGCCGACGGC CGAAGATTGG 
AAGGCTAAAA AGGCGGATTT AGTGCAAATG TGTGTTACAG TGCTCGCCAC ACTGTCCGCC 
GAGCGGCTTA AAGCCGACGG AAAGCTCTCG GCTGAGCATG TTCCAGAGGA TTTTACATAT 
TTAAGTCTGA AAGATGAAAT TGTGCCATAT ATGAATGAGA ATTGGTATAT GCTCACTGCA 
ATCAAGCAGA AAAAGGAATG GCATCAGAAT TTGGCGCCAA CTTTGCTCAA AGAGAAGAAT 
ATTTTCGTGC AACACAACGA CGATGATGAT CTATTTGCAT TGGCAGAAAA GAATTTATCA 
CTTCTAGGAC CACTACACGA GGCAGTTAAA CTAATCGGAA AACGTCCAAT CGAAAGGGAA 
AACCGTGAGC CACGTCATAT TGAGCTTCCA CCGATTGAGG GACCGAAAAC TCGTGGAGCA 
TCGAAACGCC GGCACGCCGA GGCTCCGGTG ACCGGAAAAA AGCAGAAATT AACTTCCAGA 
GCAGCCGACT ATTCATCAAC TGCAGCTCCA AATGGTGTTC AGATTGATAT ACCATTCAGT 
AAGGACAATT ATCGATATTA TCTCACTGAA GTCGATCCAA ATGTACCGGA AGACCCAGCA 
TGGAATCAAA ATCAATCATC AGCGTATGTG ATACCATCAT TTCATTATCG AGAACTATTA 
AATCCAACAG TAAATGTATC ATCAAATGAT CGTGCATTTC AATTATCGAT TAATGGAAAT 
TCAATAACTG GATTTGAAGG ATATTCAATG GCACGTGCAA GTCATGGTGT ATCTAAAGGA 
ACATGGTATT TCGAAGTTAA TTTTGATGAT CAACCTGATG ATTCACATAT TCGTATTGGA 
TGGAGTCAGT CTTATGCATC CCTGCAAGCA TGTGTTGGAT ACAACAAATT CTCATATGGA 
TGGCGATCGA AACACGGAAC GAAATTTCAT GAGGCAAAAG GAAAAAAGTA TCATTTTGGA 
GGTTTTAAGC AAGGCGACGT TCTCGGCTGT CTAATCCATC TTCCAGTGGA CAAAAAACTG 
CAGATTCCGG CAAATTTGCC ATCGGAAAAG TATCTTCCAG TATCACACAA AGGCTTCAAT 
TTGATATCAT TTAAAGCGAA TTATTTCTTT GAAGTCCAAG AGGAGTCCGC AGATATTGCA 
AAGACTCTGG TGGAAATGCC TGGAAGCTAT ATCGAGTTCT TCCACAATGG AAAATCGTGC 
GGAAAAGCAT ATGAGAATAT CTACGCCGGC GCCTATTACC CGTCGATTTC CATATTTAAA 
TCTGCCACAG CCACCATGAA TCTCGGCCCT AAATTCCGTA ATCTGCCACG TGGTGCCACC 
GGAATCCACG CGAGAGCCGA CGAGCAGCAA CACGAACAAA CTCTTTCGGA TATGCTGTAT 
TTGGTGTCAA AAGAAGTGAA TTTGGACCAT CCGCCGCGTG TAAAACGAGA GGACGATGAT 
GACGTGAAGG ATATCAAGAA AGAAATTAAA CAAGAAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ash-2 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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