top-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	top-2
Entrez Gene ID	174825
Full Name	DNA topoisomerase 2 top-2
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064135.3	NP_496536.1	DNA topoisomerase 2 top-2
NM_064135.4	NP_496536.1	DNA topoisomerase 2 top-2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Topo-II promotes chromosome-length shortening.
Title: CENP-A and topoisomerase-II antagonistically affect chromosome length.

These results explain why hTOPIIalpha and hTOPIIbeta are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses.
Title: Natural variation in a single amino acid substitution underlies physiological responses to topoisomerase II poisons.

Novel Top-2 mutation disrupts chromosome segregation during spermatogenesis.
Title: The Identification of a Novel Mutant Allele of topoisomerase II in Caenorhabditis elegans Reveals a Unique Role in Chromosome Segregation During Spermatogenesis.

The following top-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339690	NM_064135.4 Latest version!	Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa339690	NM_064135.3	Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top-2 ( NM_064135.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496536.1 Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), partial mRNA.

Price & Availability↑

CloneID	OCa339690
Clone ID Related Accession (Same CDS sequence)	NM_064135.3 , NM_064135.4
Accession Version	NM_064135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA topoisomerase 2 top-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064135.2. COMPLETENESS: incomplete on both ends.

ATGAGCGACT CTGATAGTGA ATTCTCTATC GAAGATTCAC CCAAGAAGAA AACCGCACCA 
AAGAAGGAGA AGGCGTCACC GAAGAAAAAG AAAGATGATG CCAACGAGTC GATGGTTATG 
ACTGAAGAGG ACCGCAATGT GTTCACGTCG ATCGACAAAA AAGGTGGTGG CTCGAAACAA 
ATGGCGATCG AGGATATCTA CCAAAAGAAG TCCCAACTCG AGCACATTCT TCTTCGCCCG 
GACACCTACA TCGGTTCGGT CGAGCATACC GAGAAGACGC CGATGTGGGT CTACAACATG 
GAGGAGAGCA AGCTGGAGCA GCGTGACATC TCATACGTGC CGGGACTCTA CAAGATTTAT 
GATGAAATTC TTGTGAATGC CGCTGACAAC AAACAGAGAG ATCCGAAGAT GAACACAATC 
AAGATCACCA TCAATAAGGA GAAAAATGAG ATCTCTGTCT ACAACAATGG CAAAGGAATC 
CCAGTCACTC AGCACAAAGT GGAGAAAGTC TACGTCCCAG AGCTGATTTT TGGAACTCTT 
CTAACATCTT CTAACTACAA TGACGATGAG AAGAAAGTGA CGGGAGGACG CAACGGATAT 
GGAGCAAAGC TTTGCAACAT CTTCTCCACA AAATTCACTC TGGAGACGTC TTCACGGGAT 
TACAAAAGTG CATTCAAGCA AACGTGGATC AAAAACATGA CGCGTGATGA GGAGCCGAAG 
ATTGTTAAGA GCACCGACGA AGACTTCACG AAGATCACAT TCTCGCCGGA TCTGGCAAAG 
TTCAAGATGA AGGAGCTGGA CGATGACATC TGCCATTTGA TGGCTCGGCG TGCGTATGAT 
GTGGCTGGAT CCAGTAAAGG AGTCGCCGTG TTCCTCAATG GAAAACGAAT TCCAATCAAA 
GGATTCGAGG ACTACGTCCA AATGTACACT TCGCAGTTCA ACAATGAAGG AGAACCCCTC 
AAGATCGCAT ACGAGCAAGT TGGAGATCGT TGGCAAGTCG CCTTGGCTCT TTCGGAAAAA 
GGCTTCCAAC AAGTGTCATT TGTCAACAGT ATTGCTACCA CAAAAGGAGG ACGGCACGTG 
GACTACGTGG CTGATCAGAT GGTCGCCAAG TTCATCGACT CGATCAAGCG GAAGCTCACA 
AAGACGTCGA TGAATATCAA GCCATTCCAG ATCAAAAATC ACATGTGGGT TTTTGTGAAT 
TGCTTGATCG AAAATCCGAC ATTCGACTCC CAGACGAAAG AGACTATGAC CCTGCAGCAG 
AAGCAGTTCG GATCGACCTG CGTCCTCAGC GAGAAGTTCT CCAAAGCGGC TTCAAGTGTT 
GGAATCACCG ATGCTGTTAT GAGTTGGGTT CGCTTCAAAC AAATGGACGA CCTCAACAAA 
AAGTGCTCCA AGACGAAGAC TTCCAAGCTG AAGGGCATTC CAAAGCTTGA AGATGCCAAC 
GACGCTGGAA CCAAAAATTC CCAACAATGC ACATTAATTC TGACGGAGGG AGACTCGGCC 
AAAACGCTTG CTGTTTCGGG ACTTTCAGTC GTTGGACGCG ACAAATATGG AGTATTCCCA 
CTTCGAGGAA AATTGCTAAA TGTCCGAGAA GGAAATATGA AACAAATCGC CGATAATGCT 
GAGGTCAATG CCATGATCAA AATTCTGGGA CTCCAATACA AGAAGAAGTA CGAGACAGAA 
GATGACTTCA AGACTCTTCG ATACGGTAAA CTGATGGTCA TGGCTGATCA GGATCAAGAC 
GGTTCACACA TCAAAGGACT CGTGATCAAT TTCATCCATC ACTTCTGGCC TTCTCTCATT 
CAGCGGAACT TCGTAGAAGA ATTCATCACT CCAATTGTCA AAGCCACCAA AGGAAAAGAA 
GAAGTTTCCT TCTATTCGCT CCCAGAATAT TCTGAGTGGA GAATGAATAC GGATAACTGG 
AAGTCGTACA AGATCAAGTA CTACAAGGGA TTGGGTACAT CGACTTCTAA GGAAGCTAAG 
GAATACTTCT TGGACATGGT TCGTCATCGT ATCCGCTTCA AGTACAATGG AGCTGATGAT 
GATAACGCTG TCGACATGGC TTTCTCGAAG AAGAAGATTG AAGAGAGAAA AGACTGGCTC 
TCGAAGTGGA TGCGGGAGAA GAAGGACCGA AAGCAGCAGG GACTTGCTGA AGAATATCTC 
TACAACAAGG ACACTCGATT CGTCACCTTC AAAGATTTCG TCAATCGTGA ATTGGTCCTC 
TTCTCGAATC TCGACAACGA GCGTTCAATT CCATGCCTTG TGGATGGTTT CAAGCCAGGA 
CAGCGGAAGG TTCTCTTCGC GTGCTTCAAG AGAGCAGACA AGCGTGAAGT CAAAGTAGCT 
CAATTGGCTG GAGCTGTCGC TGAAATTTCT GCTTACCATC ACGGAGAACA GTCGCTTATG 
GGAACAATTG TGAATCTCGC TCAAGATTAC GTTGGCTCCA ACAACATCAA CCTGCTTCTT 
CCAATCGGAC AGTTTGGTAC TCGTCTCCAA GGTGGAAAGG ACAGTGCTTC AGCTCGTTAC 
ATCTTCACTC AACTGTCGCC AGTCACTCGT ACACTCTTCC CGGCTCACGA TGACAATGTC 
CTTCGTTTCC TTTATGAAGA AAATCAGCGT ATTGAGCCAG AATGGTATTG CCCGATTATT 
CCAATGGTAC TCGTCAATGG AGCTCAAGGC ATCGGTACTG GATGGTCCAC GAACATTCCT 
AATTACAATC CACGTGAACT GGTCAAGAAC ATCAAGCGTC TTATCGCCGG CGAGCCACAG 
AAAGCCTTAG CTCCATGGTA CAAGAACTTC CGCGGAAAGA TCATTCAAAT CGACCCGAGT 
CGATTTGCAT GCTATGGTGA AGTTGCTGTG CTGGATGACA ACACCATTGA GATCACCGAG 
TTGCCAATCA AACAGTGGAC TCAAGACTAC AAAGAGAAGG TTTTGGAAGG ATTGATGGAG 
AGCTCGGACA AGAAATCGCC TGTTATCGTC GACTACAAAG AGTATCACAC AGATACAACT 
GTCAAGTTCG TCGTGAAGCT CTCGCCGGGA AAACTTCGTG AATTGGAGCG CGGTCAGGAT 
CTTCATCAAG TCTTCAAGCT CCAAGCTGTC ATCAACACTA CGTGCATGGT ACTTTTCGAT 
GCTGCCGGAT GTCTTCGTAC ATACACTTCG CCAGAAGCGA TTACTCAAGA GTTCTACGAT 
AGTCGCCAAG AGAAGTACGT ACAGAGAAAG GAGTACCTTC TCGGAGTTCT TCAGGCTCAG 
AGCAAGCGTC TCACTAACCA GGCTCGTTTC ATCCTCGCCA AGATCAACAA CGAGATTGTT 
CTCGAGAACA AGAAGAAGGC GGCGATTGTC GATGTTTTGA TCAAGATGAA GTTCGATGCT 
GATCCAGTGA AGAAGTGGAA GGAGGAGCAA AAACTCAAAG AGCTTCGTGA ATCTGGAGAG 
ATCGAGCTGG ACGAAGATGA TCTAGCTGCT GTCGCCGTCG AAGAGGACGA AGCCATTTCC 
TCTGCTGCGA AGGCCGTTGA AACCAAGCTA TCAGACTACG ATTACCTTGT CGGAATGGCT 
CTCATCAAGC TTTCGGAAGA AGAGAAGAAT AAGCTCATCA AGGAATCGGA GGAGAAGATG 
GCTGAGGTTC GAGTTATCGA GAAGAAGACC TGGCAAGATC TTTGGCATGA GGATCTGGAC 
AACTTCGTGT CTGAGTTGGA CAAACAGGAG GCTCGCGAGA AAGCTGATCA AGATGCCAGC 
ATCAAAAATG CCGCCAAAAA GCTTGCTGCC GACGCAAAGA CCGGACGTGG ACCCAAGAAG 
AATGTGTGCA CGGAAGTCCT TCCATCCAAA GATGGACAGC GCATTGAGCC GATGTTGGAT 
GCTGCAACCA AGGCCAAATA TGAGAAGATG TCTCAGCCAA AGAAGGAACG CGTCAAGAAA 
GAGCCAAAGG AGCCGAAAGA ACCAAAGAAG GTGAAAAAGG AAGGCCAGGA CATTAAAAAG 
TTCATGTCTC CAGCTGCTCC GAAGACTGCG AAGAAGGAGA AAAGCGACGG CTTCAACTCT 
GACATGTCAG AGGAAAGTGA CGTGGAGTTC GATGAAGGAA TCGATTTCGA TTCGGACGAT 
GACGGTGTGG AGCGTGAGGA TGTCGTCTCA AAGCCAAAAC CTCGTACTGG AAAAGGAGCT 
GCGAAAGCGG AAGTTATCGA TTTGTCAGAT GATGATGAAG TTCCTGCAAA GAAGCCTGCT 
CCAGCGAAGA AAGCTGCTCC GAAGAAGAAA AAGAGCGAGT TCAGCGATCT GTCTGGCGGC 
GACAGTGATG AAGAAGCTGA GAAGAAGCCG TCCACCAGCA AAAAGCCGTC TCCAAAGAAG 
GCTGCTCCGA AGACGGCCGA GCCCAAGTCG AAGGCGGTGA CCGATTTCTT TGGTGCTTCA 
AAGAAGAACG GAAAGAAGGC CGCTGGTTCC GATGATGAAG ACGACGAAAG CTTCGTTGTG 
GCTCCAAGAG AGAAGAGCGG AAGAGCTCGC AAGGCGCCAC CAACTTACGA CGTGGATTCA 
GGATCCGATT CGGATCAGCC AAAGAAGAAG AGGGGACGCG TCGTCGACTC CGACTCGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496536.1
CDS	1..4563
Translation	MSDSDSEFSIEDSPKKKTAPKKEKASPKKKKDDANESMVMTEEDRNVFTSIDKKGGGSKQMAIEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNEGEPLKIAYEQVGDRWQVALALSEKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLDAATKAKYEKMSQPKKERVKKEPKEPKEPKKVKKEGQDIKKFMSPAAPKTAKKEKSDGFNSDMSEESDVEFDEGIDFDSDDDGVEREDVVSKPKPRTGKGAAKAEVIDLSDDDEVPAKKPAPAKKAAPKKKKSEFSDLSGGDSDEEAEKKPSTSKKPSPKKAAPKTAEPKSKAVTDFFGASKKNGKKAAGSDDEDDESFVVAPREKSGRARKAPPTYDVDSGSDSDQPKKKRGRVVDSDSD

Target ORF information:

RefSeq Version	NM_064135.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064135.3

ORF Insert Sequence:

ATGAGCGACT CTGATAGTGA ATTCTCTATC GAAGATTCAC CCAAGAAGAA AACCGCACCA 
AAGAAGGAGA AGGCGTCACC GAAGAAAAAG AAAGATGATG CCAACGAGTC GATGGTTATG 
ACTGAAGAGG ACCGCAATGT GTTCACGTCG ATCGACAAAA AAGGTGGTGG CTCGAAACAA 
ATGGCGATCG AGGATATCTA CCAAAAGAAG TCCCAACTCG AGCACATTCT TCTTCGCCCG 
GACACCTACA TCGGTTCGGT CGAGCATACC GAGAAGACGC CGATGTGGGT CTACAACATG 
GAGGAGAGCA AGCTGGAGCA GCGTGACATC TCATACGTGC CGGGACTCTA CAAGATTTAT 
GATGAAATTC TTGTGAATGC CGCTGACAAC AAACAGAGAG ATCCGAAGAT GAACACAATC 
AAGATCACCA TCAATAAGGA GAAAAATGAG ATCTCTGTCT ACAACAATGG CAAAGGAATC 
CCAGTCACTC AGCACAAAGT GGAGAAAGTC TACGTCCCAG AGCTGATTTT TGGAACTCTT 
CTAACATCTT CTAACTACAA TGACGATGAG AAGAAAGTGA CGGGAGGACG CAACGGATAT 
GGAGCAAAGC TTTGCAACAT CTTCTCCACA AAATTCACTC TGGAGACGTC TTCACGGGAT 
TACAAAAGTG CATTCAAGCA AACGTGGATC AAAAACATGA CGCGTGATGA GGAGCCGAAG 
ATTGTTAAGA GCACCGACGA AGACTTCACG AAGATCACAT TCTCGCCGGA TCTGGCAAAG 
TTCAAGATGA AGGAGCTGGA CGATGACATC TGCCATTTGA TGGCTCGGCG TGCGTATGAT 
GTGGCTGGAT CCAGTAAAGG AGTCGCCGTG TTCCTCAATG GAAAACGAAT TCCAATCAAA 
GGATTCGAGG ACTACGTCCA AATGTACACT TCGCAGTTCA ACAATGAAGG AGAACCCCTC 
AAGATCGCAT ACGAGCAAGT TGGAGATCGT TGGCAAGTCG CCTTGGCTCT TTCGGAAAAA 
GGCTTCCAAC AAGTGTCATT TGTCAACAGT ATTGCTACCA CAAAAGGAGG ACGGCACGTG 
GACTACGTGG CTGATCAGAT GGTCGCCAAG TTCATCGACT CGATCAAGCG GAAGCTCACA 
AAGACGTCGA TGAATATCAA GCCATTCCAG ATCAAAAATC ACATGTGGGT TTTTGTGAAT 
TGCTTGATCG AAAATCCGAC ATTCGACTCC CAGACGAAAG AGACTATGAC CCTGCAGCAG 
AAGCAGTTCG GATCGACCTG CGTCCTCAGC GAGAAGTTCT CCAAAGCGGC TTCAAGTGTT 
GGAATCACCG ATGCTGTTAT GAGTTGGGTT CGCTTCAAAC AAATGGACGA CCTCAACAAA 
AAGTGCTCCA AGACGAAGAC TTCCAAGCTG AAGGGCATTC CAAAGCTTGA AGATGCCAAC 
GACGCTGGAA CCAAAAATTC CCAACAATGC ACATTAATTC TGACGGAGGG AGACTCGGCC 
AAAACGCTTG CTGTTTCGGG ACTTTCAGTC GTTGGACGCG ACAAATATGG AGTATTCCCA 
CTTCGAGGAA AATTGCTAAA TGTCCGAGAA GGAAATATGA AACAAATCGC CGATAATGCT 
GAGGTCAATG CCATGATCAA AATTCTGGGA CTCCAATACA AGAAGAAGTA CGAGACAGAA 
GATGACTTCA AGACTCTTCG ATACGGTAAA CTGATGGTCA TGGCTGATCA GGATCAAGAC 
GGTTCACACA TCAAAGGACT CGTGATCAAT TTCATCCATC ACTTCTGGCC TTCTCTCATT 
CAGCGGAACT TCGTAGAAGA ATTCATCACT CCAATTGTCA AAGCCACCAA AGGAAAAGAA 
GAAGTTTCCT TCTATTCGCT CCCAGAATAT TCTGAGTGGA GAATGAATAC GGATAACTGG 
AAGTCGTACA AGATCAAGTA CTACAAGGGA TTGGGTACAT CGACTTCTAA GGAAGCTAAG 
GAATACTTCT TGGACATGGT TCGTCATCGT ATCCGCTTCA AGTACAATGG AGCTGATGAT 
GATAACGCTG TCGACATGGC TTTCTCGAAG AAGAAGATTG AAGAGAGAAA AGACTGGCTC 
TCGAAGTGGA TGCGGGAGAA GAAGGACCGA AAGCAGCAGG GACTTGCTGA AGAATATCTC 
TACAACAAGG ACACTCGATT CGTCACCTTC AAAGATTTCG TCAATCGTGA ATTGGTCCTC 
TTCTCGAATC TCGACAACGA GCGTTCAATT CCATGCCTTG TGGATGGTTT CAAGCCAGGA 
CAGCGGAAGG TTCTCTTCGC GTGCTTCAAG AGAGCAGACA AGCGTGAAGT CAAAGTAGCT 
CAATTGGCTG GAGCTGTCGC TGAAATTTCT GCTTACCATC ACGGAGAACA GTCGCTTATG 
GGAACAATTG TGAATCTCGC TCAAGATTAC GTTGGCTCCA ACAACATCAA CCTGCTTCTT 
CCAATCGGAC AGTTTGGTAC TCGTCTCCAA GGTGGAAAGG ACAGTGCTTC AGCTCGTTAC 
ATCTTCACTC AACTGTCGCC AGTCACTCGT ACACTCTTCC CGGCTCACGA TGACAATGTC 
CTTCGTTTCC TTTATGAAGA AAATCAGCGT ATTGAGCCAG AATGGTATTG CCCGATTATT 
CCAATGGTAC TCGTCAATGG AGCTCAAGGC ATCGGTACTG GATGGTCCAC GAACATTCCT 
AATTACAATC CACGTGAACT GGTCAAGAAC ATCAAGCGTC TTATCGCCGG CGAGCCACAG 
AAAGCCTTAG CTCCATGGTA CAAGAACTTC CGCGGAAAGA TCATTCAAAT CGACCCGAGT 
CGATTTGCAT GCTATGGTGA AGTTGCTGTG CTGGATGACA ACACCATTGA GATCACCGAG 
TTGCCAATCA AACAGTGGAC TCAAGACTAC AAAGAGAAGG TTTTGGAAGG ATTGATGGAG 
AGCTCGGACA AGAAATCGCC TGTTATCGTC GACTACAAAG AGTATCACAC AGATACAACT 
GTCAAGTTCG TCGTGAAGCT CTCGCCGGGA AAACTTCGTG AATTGGAGCG CGGTCAGGAT 
CTTCATCAAG TCTTCAAGCT CCAAGCTGTC ATCAACACTA CGTGCATGGT ACTTTTCGAT 
GCTGCCGGAT GTCTTCGTAC ATACACTTCG CCAGAAGCGA TTACTCAAGA GTTCTACGAT 
AGTCGCCAAG AGAAGTACGT ACAGAGAAAG GAGTACCTTC TCGGAGTTCT TCAGGCTCAG 
AGCAAGCGTC TCACTAACCA GGCTCGTTTC ATCCTCGCCA AGATCAACAA CGAGATTGTT 
CTCGAGAACA AGAAGAAGGC GGCGATTGTC GATGTTTTGA TCAAGATGAA GTTCGATGCT 
GATCCAGTGA AGAAGTGGAA GGAGGAGCAA AAACTCAAAG AGCTTCGTGA ATCTGGAGAG 
ATCGAGCTGG ACGAAGATGA TCTAGCTGCT GTCGCCGTCG AAGAGGACGA AGCCATTTCC 
TCTGCTGCGA AGGCCGTTGA AACCAAGCTA TCAGACTACG ATTACCTTGT CGGAATGGCT 
CTCATCAAGC TTTCGGAAGA AGAGAAGAAT AAGCTCATCA AGGAATCGGA GGAGAAGATG 
GCTGAGGTTC GAGTTATCGA GAAGAAGACC TGGCAAGATC TTTGGCATGA GGATCTGGAC 
AACTTCGTGT CTGAGTTGGA CAAACAGGAG GCTCGCGAGA AAGCTGATCA AGATGCCAGC 
ATCAAAAATG CCGCCAAAAA GCTTGCTGCC GACGCAAAGA CCGGACGTGG ACCCAAGAAG 
AATGTGTGCA CGGAAGTCCT TCCATCCAAA GATGGACAGC GCATTGAGCC GATGTTGGAT 
GCTGCAACCA AGGCCAAATA TGAGAAGATG TCTCAGCCAA AGAAGGAACG CGTCAAGAAA 
GAGCCAAAGG AGCCGAAAGA ACCAAAGAAG GTGAAAAAGG AAGGCCAGGA CATTAAAAAG 
TTCATGTCTC CAGCTGCTCC GAAGACTGCG AAGAAGGAGA AAAGCGACGG CTTCAACTCT 
GACATGTCAG AGGAAAGTGA CGTGGAGTTC GATGAAGGAA TCGATTTCGA TTCGGACGAT 
GACGGTGTGG AGCGTGAGGA TGTCGTCTCA AAGCCAAAAC CTCGTACTGG AAAAGGAGCT 
GCGAAAGCGG AAGTTATCGA TTTGTCAGAT GATGATGAAG TTCCTGCAAA GAAGCCTGCT 
CCAGCGAAGA AAGCTGCTCC GAAGAAGAAA AAGAGCGAGT TCAGCGATCT GTCTGGCGGC 
GACAGTGATG AAGAAGCTGA GAAGAAGCCG TCCACCAGCA AAAAGCCGTC TCCAAAGAAG 
GCTGCTCCGA AGACGGCCGA GCCCAAGTCG AAGGCGGTGA CCGATTTCTT TGGTGCTTCA 
AAGAAGAACG GAAAGAAGGC CGCTGGTTCC GATGATGAAG ACGACGAAAG CTTCGTTGTG 
GCTCCAAGAG AGAAGAGCGG AAGAGCTCGC AAGGCGCCAC CAACTTACGA CGTGGATTCA 
GGATCCGATT CGGATCAGCC AAAGAAGAAG AGGGGACGCG TCGTCGACTC CGACTCGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top-2 ( NM_064135.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496536.1 Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), mRNA.

Price & Availability↑

CloneID	OCa339690
Clone ID Related Accession (Same CDS sequence)	NM_064135.3 , NM_064135.4
Accession Version	NM_064135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA topoisomerase 2 top-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064135.3.

ATGAGCGACT CTGATAGTGA ATTCTCTATC GAAGATTCAC CCAAGAAGAA AACCGCACCA 
AAGAAGGAGA AGGCGTCACC GAAGAAAAAG AAAGATGATG CCAACGAGTC GATGGTTATG 
ACTGAAGAGG ACCGCAATGT GTTCACGTCG ATCGACAAAA AAGGTGGTGG CTCGAAACAA 
ATGGCGATCG AGGATATCTA CCAAAAGAAG TCCCAACTCG AGCACATTCT TCTTCGCCCG 
GACACCTACA TCGGTTCGGT CGAGCATACC GAGAAGACGC CGATGTGGGT CTACAACATG 
GAGGAGAGCA AGCTGGAGCA GCGTGACATC TCATACGTGC CGGGACTCTA CAAGATTTAT 
GATGAAATTC TTGTGAATGC CGCTGACAAC AAACAGAGAG ATCCGAAGAT GAACACAATC 
AAGATCACCA TCAATAAGGA GAAAAATGAG ATCTCTGTCT ACAACAATGG CAAAGGAATC 
CCAGTCACTC AGCACAAAGT GGAGAAAGTC TACGTCCCAG AGCTGATTTT TGGAACTCTT 
CTAACATCTT CTAACTACAA TGACGATGAG AAGAAAGTGA CGGGAGGACG CAACGGATAT 
GGAGCAAAGC TTTGCAACAT CTTCTCCACA AAATTCACTC TGGAGACGTC TTCACGGGAT 
TACAAAAGTG CATTCAAGCA AACGTGGATC AAAAACATGA CGCGTGATGA GGAGCCGAAG 
ATTGTTAAGA GCACCGACGA AGACTTCACG AAGATCACAT TCTCGCCGGA TCTGGCAAAG 
TTCAAGATGA AGGAGCTGGA CGATGACATC TGCCATTTGA TGGCTCGGCG TGCGTATGAT 
GTGGCTGGAT CCAGTAAAGG AGTCGCCGTG TTCCTCAATG GAAAACGAAT TCCAATCAAA 
GGATTCGAGG ACTACGTCCA AATGTACACT TCGCAGTTCA ACAATGAAGG AGAACCCCTC 
AAGATCGCAT ACGAGCAAGT TGGAGATCGT TGGCAAGTCG CCTTGGCTCT TTCGGAAAAA 
GGCTTCCAAC AAGTGTCATT TGTCAACAGT ATTGCTACCA CAAAAGGAGG ACGGCACGTG 
GACTACGTGG CTGATCAGAT GGTCGCCAAG TTCATCGACT CGATCAAGCG GAAGCTCACA 
AAGACGTCGA TGAATATCAA GCCATTCCAG ATCAAAAATC ACATGTGGGT TTTTGTGAAT 
TGCTTGATCG AAAATCCGAC ATTCGACTCC CAGACGAAAG AGACTATGAC CCTGCAGCAG 
AAGCAGTTCG GATCGACCTG CGTCCTCAGC GAGAAGTTCT CCAAAGCGGC TTCAAGTGTT 
GGAATCACCG ATGCTGTTAT GAGTTGGGTT CGCTTCAAAC AAATGGACGA CCTCAACAAA 
AAGTGCTCCA AGACGAAGAC TTCCAAGCTG AAGGGCATTC CAAAGCTTGA AGATGCCAAC 
GACGCTGGAA CCAAAAATTC CCAACAATGC ACATTAATTC TGACGGAGGG AGACTCGGCC 
AAAACGCTTG CTGTTTCGGG ACTTTCAGTC GTTGGACGCG ACAAATATGG AGTATTCCCA 
CTTCGAGGAA AATTGCTAAA TGTCCGAGAA GGAAATATGA AACAAATCGC CGATAATGCT 
GAGGTCAATG CCATGATCAA AATTCTGGGA CTCCAATACA AGAAGAAGTA CGAGACAGAA 
GATGACTTCA AGACTCTTCG ATACGGTAAA CTGATGGTCA TGGCTGATCA GGATCAAGAC 
GGTTCACACA TCAAAGGACT CGTGATCAAT TTCATCCATC ACTTCTGGCC TTCTCTCATT 
CAGCGGAACT TCGTAGAAGA ATTCATCACT CCAATTGTCA AAGCCACCAA AGGAAAAGAA 
GAAGTTTCCT TCTATTCGCT CCCAGAATAT TCTGAGTGGA GAATGAATAC GGATAACTGG 
AAGTCGTACA AGATCAAGTA CTACAAGGGA TTGGGTACAT CGACTTCTAA GGAAGCTAAG 
GAATACTTCT TGGACATGGT TCGTCATCGT ATCCGCTTCA AGTACAATGG AGCTGATGAT 
GATAACGCTG TCGACATGGC TTTCTCGAAG AAGAAGATTG AAGAGAGAAA AGACTGGCTC 
TCGAAGTGGA TGCGGGAGAA GAAGGACCGA AAGCAGCAGG GACTTGCTGA AGAATATCTC 
TACAACAAGG ACACTCGATT CGTCACCTTC AAAGATTTCG TCAATCGTGA ATTGGTCCTC 
TTCTCGAATC TCGACAACGA GCGTTCAATT CCATGCCTTG TGGATGGTTT CAAGCCAGGA 
CAGCGGAAGG TTCTCTTCGC GTGCTTCAAG AGAGCAGACA AGCGTGAAGT CAAAGTAGCT 
CAATTGGCTG GAGCTGTCGC TGAAATTTCT GCTTACCATC ACGGAGAACA GTCGCTTATG 
GGAACAATTG TGAATCTCGC TCAAGATTAC GTTGGCTCCA ACAACATCAA CCTGCTTCTT 
CCAATCGGAC AGTTTGGTAC TCGTCTCCAA GGTGGAAAGG ACAGTGCTTC AGCTCGTTAC 
ATCTTCACTC AACTGTCGCC AGTCACTCGT ACACTCTTCC CGGCTCACGA TGACAATGTC 
CTTCGTTTCC TTTATGAAGA AAATCAGCGT ATTGAGCCAG AATGGTATTG CCCGATTATT 
CCAATGGTAC TCGTCAATGG AGCTCAAGGC ATCGGTACTG GATGGTCCAC GAACATTCCT 
AATTACAATC CACGTGAACT GGTCAAGAAC ATCAAGCGTC TTATCGCCGG CGAGCCACAG 
AAAGCCTTAG CTCCATGGTA CAAGAACTTC CGCGGAAAGA TCATTCAAAT CGACCCGAGT 
CGATTTGCAT GCTATGGTGA AGTTGCTGTG CTGGATGACA ACACCATTGA GATCACCGAG 
TTGCCAATCA AACAGTGGAC TCAAGACTAC AAAGAGAAGG TTTTGGAAGG ATTGATGGAG 
AGCTCGGACA AGAAATCGCC TGTTATCGTC GACTACAAAG AGTATCACAC AGATACAACT 
GTCAAGTTCG TCGTGAAGCT CTCGCCGGGA AAACTTCGTG AATTGGAGCG CGGTCAGGAT 
CTTCATCAAG TCTTCAAGCT CCAAGCTGTC ATCAACACTA CGTGCATGGT ACTTTTCGAT 
GCTGCCGGAT GTCTTCGTAC ATACACTTCG CCAGAAGCGA TTACTCAAGA GTTCTACGAT 
AGTCGCCAAG AGAAGTACGT ACAGAGAAAG GAGTACCTTC TCGGAGTTCT TCAGGCTCAG 
AGCAAGCGTC TCACTAACCA GGCTCGTTTC ATCCTCGCCA AGATCAACAA CGAGATTGTT 
CTCGAGAACA AGAAGAAGGC GGCGATTGTC GATGTTTTGA TCAAGATGAA GTTCGATGCT 
GATCCAGTGA AGAAGTGGAA GGAGGAGCAA AAACTCAAAG AGCTTCGTGA ATCTGGAGAG 
ATCGAGCTGG ACGAAGATGA TCTAGCTGCT GTCGCCGTCG AAGAGGACGA AGCCATTTCC 
TCTGCTGCGA AGGCCGTTGA AACCAAGCTA TCAGACTACG ATTACCTTGT CGGAATGGCT 
CTCATCAAGC TTTCGGAAGA AGAGAAGAAT AAGCTCATCA AGGAATCGGA GGAGAAGATG 
GCTGAGGTTC GAGTTATCGA GAAGAAGACC TGGCAAGATC TTTGGCATGA GGATCTGGAC 
AACTTCGTGT CTGAGTTGGA CAAACAGGAG GCTCGCGAGA AAGCTGATCA AGATGCCAGC 
ATCAAAAATG CCGCCAAAAA GCTTGCTGCC GACGCAAAGA CCGGACGTGG ACCCAAGAAG 
AATGTGTGCA CGGAAGTCCT TCCATCCAAA GATGGACAGC GCATTGAGCC GATGTTGGAT 
GCTGCAACCA AGGCCAAATA TGAGAAGATG TCTCAGCCAA AGAAGGAACG CGTCAAGAAA 
GAGCCAAAGG AGCCGAAAGA ACCAAAGAAG GTGAAAAAGG AAGGCCAGGA CATTAAAAAG 
TTCATGTCTC CAGCTGCTCC GAAGACTGCG AAGAAGGAGA AAAGCGACGG CTTCAACTCT 
GACATGTCAG AGGAAAGTGA CGTGGAGTTC GATGAAGGAA TCGATTTCGA TTCGGACGAT 
GACGGTGTGG AGCGTGAGGA TGTCGTCTCA AAGCCAAAAC CTCGTACTGG AAAAGGAGCT 
GCGAAAGCGG AAGTTATCGA TTTGTCAGAT GATGATGAAG TTCCTGCAAA GAAGCCTGCT 
CCAGCGAAGA AAGCTGCTCC GAAGAAGAAA AAGAGCGAGT TCAGCGATCT GTCTGGCGGC 
GACAGTGATG AAGAAGCTGA GAAGAAGCCG TCCACCAGCA AAAAGCCGTC TCCAAAGAAG 
GCTGCTCCGA AGACGGCCGA GCCCAAGTCG AAGGCGGTGA CCGATTTCTT TGGTGCTTCA 
AAGAAGAACG GAAAGAAGGC CGCTGGTTCC GATGATGAAG ACGACGAAAG CTTCGTTGTG 
GCTCCAAGAG AGAAGAGCGG AAGAGCTCGC AAGGCGCCAC CAACTTACGA CGTGGATTCA 
GGATCCGATT CGGATCAGCC AAAGAAGAAG AGGGGACGCG TCGTCGACTC CGACTCGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496536.1
CDS	13..4575
Translation	MSDSDSEFSIEDSPKKKTAPKKEKASPKKKKDDANESMVMTEEDRNVFTSIDKKGGGSKQMAIEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNEGEPLKIAYEQVGDRWQVALALSEKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLDAATKAKYEKMSQPKKERVKKEPKEPKEPKKVKKEGQDIKKFMSPAAPKTAKKEKSDGFNSDMSEESDVEFDEGIDFDSDDDGVEREDVVSKPKPRTGKGAAKAEVIDLSDDDEVPAKKPAPAKKAAPKKKKSEFSDLSGGDSDEEAEKKPSTSKKPSPKKAAPKTAEPKSKAVTDFFGASKKNGKKAAGSDDEDDESFVVAPREKSGRARKAPPTYDVDSGSDSDQPKKKRGRVVDSDSD

Target ORF information:

RefSeq Version	NM_064135.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA topoisomerase 2 top-2 (top-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064135.4

ORF Insert Sequence:

ATGAGCGACT CTGATAGTGA ATTCTCTATC GAAGATTCAC CCAAGAAGAA AACCGCACCA 
AAGAAGGAGA AGGCGTCACC GAAGAAAAAG AAAGATGATG CCAACGAGTC GATGGTTATG 
ACTGAAGAGG ACCGCAATGT GTTCACGTCG ATCGACAAAA AAGGTGGTGG CTCGAAACAA 
ATGGCGATCG AGGATATCTA CCAAAAGAAG TCCCAACTCG AGCACATTCT TCTTCGCCCG 
GACACCTACA TCGGTTCGGT CGAGCATACC GAGAAGACGC CGATGTGGGT CTACAACATG 
GAGGAGAGCA AGCTGGAGCA GCGTGACATC TCATACGTGC CGGGACTCTA CAAGATTTAT 
GATGAAATTC TTGTGAATGC CGCTGACAAC AAACAGAGAG ATCCGAAGAT GAACACAATC 
AAGATCACCA TCAATAAGGA GAAAAATGAG ATCTCTGTCT ACAACAATGG CAAAGGAATC 
CCAGTCACTC AGCACAAAGT GGAGAAAGTC TACGTCCCAG AGCTGATTTT TGGAACTCTT 
CTAACATCTT CTAACTACAA TGACGATGAG AAGAAAGTGA CGGGAGGACG CAACGGATAT 
GGAGCAAAGC TTTGCAACAT CTTCTCCACA AAATTCACTC TGGAGACGTC TTCACGGGAT 
TACAAAAGTG CATTCAAGCA AACGTGGATC AAAAACATGA CGCGTGATGA GGAGCCGAAG 
ATTGTTAAGA GCACCGACGA AGACTTCACG AAGATCACAT TCTCGCCGGA TCTGGCAAAG 
TTCAAGATGA AGGAGCTGGA CGATGACATC TGCCATTTGA TGGCTCGGCG TGCGTATGAT 
GTGGCTGGAT CCAGTAAAGG AGTCGCCGTG TTCCTCAATG GAAAACGAAT TCCAATCAAA 
GGATTCGAGG ACTACGTCCA AATGTACACT TCGCAGTTCA ACAATGAAGG AGAACCCCTC 
AAGATCGCAT ACGAGCAAGT TGGAGATCGT TGGCAAGTCG CCTTGGCTCT TTCGGAAAAA 
GGCTTCCAAC AAGTGTCATT TGTCAACAGT ATTGCTACCA CAAAAGGAGG ACGGCACGTG 
GACTACGTGG CTGATCAGAT GGTCGCCAAG TTCATCGACT CGATCAAGCG GAAGCTCACA 
AAGACGTCGA TGAATATCAA GCCATTCCAG ATCAAAAATC ACATGTGGGT TTTTGTGAAT 
TGCTTGATCG AAAATCCGAC ATTCGACTCC CAGACGAAAG AGACTATGAC CCTGCAGCAG 
AAGCAGTTCG GATCGACCTG CGTCCTCAGC GAGAAGTTCT CCAAAGCGGC TTCAAGTGTT 
GGAATCACCG ATGCTGTTAT GAGTTGGGTT CGCTTCAAAC AAATGGACGA CCTCAACAAA 
AAGTGCTCCA AGACGAAGAC TTCCAAGCTG AAGGGCATTC CAAAGCTTGA AGATGCCAAC 
GACGCTGGAA CCAAAAATTC CCAACAATGC ACATTAATTC TGACGGAGGG AGACTCGGCC 
AAAACGCTTG CTGTTTCGGG ACTTTCAGTC GTTGGACGCG ACAAATATGG AGTATTCCCA 
CTTCGAGGAA AATTGCTAAA TGTCCGAGAA GGAAATATGA AACAAATCGC CGATAATGCT 
GAGGTCAATG CCATGATCAA AATTCTGGGA CTCCAATACA AGAAGAAGTA CGAGACAGAA 
GATGACTTCA AGACTCTTCG ATACGGTAAA CTGATGGTCA TGGCTGATCA GGATCAAGAC 
GGTTCACACA TCAAAGGACT CGTGATCAAT TTCATCCATC ACTTCTGGCC TTCTCTCATT 
CAGCGGAACT TCGTAGAAGA ATTCATCACT CCAATTGTCA AAGCCACCAA AGGAAAAGAA 
GAAGTTTCCT TCTATTCGCT CCCAGAATAT TCTGAGTGGA GAATGAATAC GGATAACTGG 
AAGTCGTACA AGATCAAGTA CTACAAGGGA TTGGGTACAT CGACTTCTAA GGAAGCTAAG 
GAATACTTCT TGGACATGGT TCGTCATCGT ATCCGCTTCA AGTACAATGG AGCTGATGAT 
GATAACGCTG TCGACATGGC TTTCTCGAAG AAGAAGATTG AAGAGAGAAA AGACTGGCTC 
TCGAAGTGGA TGCGGGAGAA GAAGGACCGA AAGCAGCAGG GACTTGCTGA AGAATATCTC 
TACAACAAGG ACACTCGATT CGTCACCTTC AAAGATTTCG TCAATCGTGA ATTGGTCCTC 
TTCTCGAATC TCGACAACGA GCGTTCAATT CCATGCCTTG TGGATGGTTT CAAGCCAGGA 
CAGCGGAAGG TTCTCTTCGC GTGCTTCAAG AGAGCAGACA AGCGTGAAGT CAAAGTAGCT 
CAATTGGCTG GAGCTGTCGC TGAAATTTCT GCTTACCATC ACGGAGAACA GTCGCTTATG 
GGAACAATTG TGAATCTCGC TCAAGATTAC GTTGGCTCCA ACAACATCAA CCTGCTTCTT 
CCAATCGGAC AGTTTGGTAC TCGTCTCCAA GGTGGAAAGG ACAGTGCTTC AGCTCGTTAC 
ATCTTCACTC AACTGTCGCC AGTCACTCGT ACACTCTTCC CGGCTCACGA TGACAATGTC 
CTTCGTTTCC TTTATGAAGA AAATCAGCGT ATTGAGCCAG AATGGTATTG CCCGATTATT 
CCAATGGTAC TCGTCAATGG AGCTCAAGGC ATCGGTACTG GATGGTCCAC GAACATTCCT 
AATTACAATC CACGTGAACT GGTCAAGAAC ATCAAGCGTC TTATCGCCGG CGAGCCACAG 
AAAGCCTTAG CTCCATGGTA CAAGAACTTC CGCGGAAAGA TCATTCAAAT CGACCCGAGT 
CGATTTGCAT GCTATGGTGA AGTTGCTGTG CTGGATGACA ACACCATTGA GATCACCGAG 
TTGCCAATCA AACAGTGGAC TCAAGACTAC AAAGAGAAGG TTTTGGAAGG ATTGATGGAG 
AGCTCGGACA AGAAATCGCC TGTTATCGTC GACTACAAAG AGTATCACAC AGATACAACT 
GTCAAGTTCG TCGTGAAGCT CTCGCCGGGA AAACTTCGTG AATTGGAGCG CGGTCAGGAT 
CTTCATCAAG TCTTCAAGCT CCAAGCTGTC ATCAACACTA CGTGCATGGT ACTTTTCGAT 
GCTGCCGGAT GTCTTCGTAC ATACACTTCG CCAGAAGCGA TTACTCAAGA GTTCTACGAT 
AGTCGCCAAG AGAAGTACGT ACAGAGAAAG GAGTACCTTC TCGGAGTTCT TCAGGCTCAG 
AGCAAGCGTC TCACTAACCA GGCTCGTTTC ATCCTCGCCA AGATCAACAA CGAGATTGTT 
CTCGAGAACA AGAAGAAGGC GGCGATTGTC GATGTTTTGA TCAAGATGAA GTTCGATGCT 
GATCCAGTGA AGAAGTGGAA GGAGGAGCAA AAACTCAAAG AGCTTCGTGA ATCTGGAGAG 
ATCGAGCTGG ACGAAGATGA TCTAGCTGCT GTCGCCGTCG AAGAGGACGA AGCCATTTCC 
TCTGCTGCGA AGGCCGTTGA AACCAAGCTA TCAGACTACG ATTACCTTGT CGGAATGGCT 
CTCATCAAGC TTTCGGAAGA AGAGAAGAAT AAGCTCATCA AGGAATCGGA GGAGAAGATG 
GCTGAGGTTC GAGTTATCGA GAAGAAGACC TGGCAAGATC TTTGGCATGA GGATCTGGAC 
AACTTCGTGT CTGAGTTGGA CAAACAGGAG GCTCGCGAGA AAGCTGATCA AGATGCCAGC 
ATCAAAAATG CCGCCAAAAA GCTTGCTGCC GACGCAAAGA CCGGACGTGG ACCCAAGAAG 
AATGTGTGCA CGGAAGTCCT TCCATCCAAA GATGGACAGC GCATTGAGCC GATGTTGGAT 
GCTGCAACCA AGGCCAAATA TGAGAAGATG TCTCAGCCAA AGAAGGAACG CGTCAAGAAA 
GAGCCAAAGG AGCCGAAAGA ACCAAAGAAG GTGAAAAAGG AAGGCCAGGA CATTAAAAAG 
TTCATGTCTC CAGCTGCTCC GAAGACTGCG AAGAAGGAGA AAAGCGACGG CTTCAACTCT 
GACATGTCAG AGGAAAGTGA CGTGGAGTTC GATGAAGGAA TCGATTTCGA TTCGGACGAT 
GACGGTGTGG AGCGTGAGGA TGTCGTCTCA AAGCCAAAAC CTCGTACTGG AAAAGGAGCT 
GCGAAAGCGG AAGTTATCGA TTTGTCAGAT GATGATGAAG TTCCTGCAAA GAAGCCTGCT 
CCAGCGAAGA AAGCTGCTCC GAAGAAGAAA AAGAGCGAGT TCAGCGATCT GTCTGGCGGC 
GACAGTGATG AAGAAGCTGA GAAGAAGCCG TCCACCAGCA AAAAGCCGTC TCCAAAGAAG 
GCTGCTCCGA AGACGGCCGA GCCCAAGTCG AAGGCGGTGA CCGATTTCTT TGGTGCTTCA 
AAGAAGAACG GAAAGAAGGC CGCTGGTTCC GATGATGAAG ACGACGAAAG CTTCGTTGTG 
GCTCCAAGAG AGAAGAGCGG AAGAGCTCGC AAGGCGCCAC CAACTTACGA CGTGGATTCA 
GGATCCGATT CGGATCAGCC AAAGAAGAAG AGGGGACGCG TCGTCGACTC CGACTCGGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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top-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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