unc-53 gene cDNA ORF clone, Caenorhabditis elegans

mRNA	Protein	Name
NM_001026999.5	NP_001022170.1	Adapter protein unc-53
NM_001026998.4	NP_001022169.1	Adapter protein unc-53
NM_001027000.4	NP_001022171.1	Adapter protein unc-53
NM_001267343.2	NP_001254272.1	Calponin-homology (CH) domain-containing protein
NM_001267344.2	NP_001254273.1	Adapter protein unc-53
NM_171039.2	NP_741045.1	Adapter protein unc-53
NM_001267342.1	NP_001254271.1	Adapter protein unc-53
NM_001027001.3	NP_001022172.1	Adapter protein unc-53
NM_001267344.1	NP_001254273.1	Adapter protein unc-53
NM_001026998.3	NP_001022169.1	Adapter protein unc-53
NM_001027000.3	NP_001022171.1	Adapter protein unc-53
NM_171039.3	NP_741045.1	Adapter protein unc-53
NM_001026999.6	NP_001022170.1	Adapter protein unc-53
NM_001267342.2	NP_001254271.1	Calponin-homology (CH) domain-containing protein
NM_001267343.1	NP_001254272.1	Adapter protein unc-53
NM_001027001.4	NP_001022172.1	Adapter protein unc-53

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339827	NM_001027001.4 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa351863	NM_001267344.2 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa337678	NM_001026999.6 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa338199	NM_171039.3 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa338200	NM_001026998.4 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa339828	NM_001027000.4 Latest version!	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa351861	NM_001267342.2 Latest version!	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa351862	NM_001267343.2 Latest version!	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa351863	NM_001267344.1	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa338200	NM_001026998.3	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa339828	NM_001027000.3	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa338199	NM_171039.2	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa337678	NM_001026999.5	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa351861	NM_001267342.1	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa351862	NM_001267343.1	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa339827	NM_001027001.3	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

unc-53 ( NM_001027001.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022172.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Price & Availability↑

CloneID	OCa339827
Clone ID Related Accession (Same CDS sequence)	NM_001027001.3 , NM_001027001.4
Accession Version	NM_001027001.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027001.2. COMPLETENESS: incomplete on both ends.

ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022172.1
CDS	1..3705
Translation	MHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001027001.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027001.3

ORF Insert Sequence:

ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001027001.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022172.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Price & Availability↑

CloneID	OCa339827
Clone ID Related Accession (Same CDS sequence)	NM_001027001.3 , NM_001027001.4
Accession Version	NM_001027001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027001.3. COMPLETENESS: incomplete on the 5' end.

ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022172.1
CDS	1..3705
Translation	MHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001027001.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027001.4

ORF Insert Sequence:

ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267344.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254273.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa351863
Clone ID Related Accession (Same CDS sequence)	NM_001267344.2 , NM_001267344.1
Accession Version	NM_001267344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267344.1. COMPLETENESS: incomplete on the 5' end.

ATGCTGAAAT TAAAGTTATT CAGTAGCAAA AACCCATCTT CCTCATCGAA TAGCCCACAA 
CCTACGAGAA AGGCGGCGGC GGTGCCTCAA CAACAAACTT TGTCGAAAAT CGCTGCCCCA 
GTGAAAAGTG GCCTGAAGCC GCCGACCAGT AAGCTGGGAA GTGCCACGTC TATGTCGAAG 
CTTTGTACGC CAAAAGTTTC CTACCGTAAA ACGGACGCCC CAATCATATC TCAACAAGAC 
TCGAAACGAT GCTCAAAGAG CAGTGAAGAA GAGTCCGGAT ACGCTGGATT CAACAGCACG 
TCGCCAACGT CATCATCGAC GGAAGGTTCC CTAAGCATGC ATTCCACATC TTCCAAGAGT 
TCAACGTCAG ACGAAAAGTC TCCGTCATCA GACGATCTTA CTCTTAACGC CTCCATCGTG 
ACAGCTATCA GACAGCCGAT AGCCGCAACA CCGGTTTCTC CAAATATTAT CAACAAGCCT 
GTTGAGGAAA AACCAACACT GGCAGTGAAA GGAGTGAAAA GCACAGCGAA AAAAGATCCA 
CCTCCAGCTG TTCCGCCACG TGACACCCAG CCAACAATCG GAGTTGTTAG TCCAATTATG 
GCACATAAGA AGTTGACAAA TGACCCCGTG ATATCTGAAA AACCAGAACC TGAAAAGCTC 
CAATCAATGA GCATCGACAC GACGGACGTT CCACCGCTTC CACCTCTAAA ATCAGTTGTT 
CCACTTAAAA TGACTTCAAT CCGACAACCA CCAACGTACG ATGTTCTTCT AAAACAAGGA 
AAAATCACAT CGCCTGTCAA GTCGTTTGGA TATGAGCAGT CGTCCGCGTC TGAAGACTCC 
ATTGTGGCTC ATGCGTCGGC TCAGGTGACT CCGCCGACAA AAACTTCTGG TAATCATTCG 
CTGGAGAGAA GGATGGGAAA GAATAAGACA TCAGAATCCA GCGGCTACAC CTCTGACGCC 
GGTGTTGCGA TGTGCGCCAA AATGAGGGAG AAGCTGAAAG AATACGATGA CATGACTCGT 
CGAGCACAGA ACGGCTATCC TGACAACTTC GAAGACAGTT CCTCCTTGTC GTCTGGAATA 
TCCGATAACA ACGAGCTCGA CGACATATCC ACGGACGATT TGTCCGGAGT AGACATGGCA 
ACAGTCGCCT CCAAACATAG CGACTATTCC CACTTTGTTC GCCATCCCAC GTCTTCTTCC 
TCAAAGCCCC GAGTCCCCAG TCGGTCCTCC ACATCAGTCG ATTCTCGATC TCGAGCAGAA 
CAGGAGAATG TGTACAAACT TCTGTCCCAG TGCCGAACGA GCCAACGTGG CGCCGCTGCC 
ACCTCAACCT TCGGACAACA TTCGCTAAGA TCCCCGGGAT ACTCATCCTA TTCTCCACAC 
TTATCAGTGT CAGCTGATAA GGACACAATG TCTATGCACT CACAGACTAG TCGACGACCT 
TCTTCACAAA AACCAAGCTA TTCAGGCCAA TTTCATTCAC TTGATCGTAA ATGCCACCTT 
CAAGAGTTCA CATCCACCGA GCACAGAATG GCGGCTCTCT TGAGCCCGAG ACGGGTGCCG 
AACTCGATGT CGAAATATGA TTCTTCAGCA GCCGCCCTCA ACGCTTCGGG TATGTCTCGT 
TCAATGATCC TTCTCGAATC CCTTTCACCA CGACCACCTC GACGTCACCA ATCACCGGCT 
GACTCGTGCA TTATCACAGC TTCTCCATCA GCCCCACGAA GATCACATTC GCCACGTGGC 
CCCACCGCCC GTATCCCACT TTCTCTCGCT TCTTCGCCTG TCCACGTCAA TAACAATTGG 
GGATCCTACT CGGCGCGTTC CCGAGGTGGA AGCTCTACTG GTATCTATGG AGAGACGTTC 
CAACTGCACA GACTATCCGA TGAAAAATCC CCCGCACATT CTGCCAAAAG TGAGATGGGA 
TCCCAACTAT CACTGGCTAG CACGACAGCA TATGGATCTC TCAATGAGAA GTACGAACAT 
GCTATTCGGG ACATGGCACG TGACTTGGAG TGTTACAAGA ACACTGTCGA CTCACTAACC 
AAGAAACAGG AGAACTATGG AGCATTGTTT GATCTTTTTG AGCAAAAGCT TAGAAAACTC 
ACTCAACACA TTGATCGATC CAACTTGAAG CCTGAAGAGG CAATACGATT CAGGCAGGAC 
ATTGCTCATT TGAGGGATAT TAGCAATCAT CTTGCATCCA ACTCAGCTCA TGCTAACGAA 
GGCGCTGGTG AGCTTCTTCG TCAACCATCT CTGGAATCAG TTGCATCCCA TCGATCATCG 
ATGTCATCGT CGTCGAAAAG CAGCAAGCAG GAGAAGATCA GCTTGAGCTC GTTTGGCAAG 
AACAAGAAGA GCTGGATCCG CTCCTCACTC TCCAAGTTCA CCAAGAAGAA GAACAAGAAC 
TACGACGAAG CACATATGCC ATCAATTTCC GGATCTCAAG GAACTCTTGA CAACATTGAT 
GTGATTGAGT TGAAGCAAGA GCTCAAAGAA CGCGATAGTG CACTTTACGA AGTCCGCCTT 
GACAATCTGG ATCGTGCCCG CGAAGTTGAT GTTCTGAGGG AGACAGTGAA CAAGTTGAAA 
ACCGAGAACA AGCAATTAAA GAAAGAAGTG GACAAACTCA CCAACGGTCC AGCCACTCGT 
GCTTCTTCCC GCGCCTCAAT TCCAGTTATC TACGACGATG AGCATGTCTA TGATGCAGCG 
TGTAGCAGTA CATCAGCTAG TCAATCTTCG AAACGATCCT CTGGCTGCAA CTCAATCAAG 
GTTACTGTAA ACGTGGACAT CGCTGGAGAA ATCAGTTCGA TCGTTAACCC GGACAAAGAG 
ATAATCGTAG GATATCTTGC CATGCCAACC AGTCAGTCAT GCTGGAAAGA CATTGATGTT 
TCTATTCTAG GACTATTTGA AGTCTACCTA TCCAGAATTG ATGTGGAGCA TCAACTTGGA 
ATCGATGCTC GTGATTCTAT CCTTGGCTAT CAAATTGGTG AACTTCGACG CGTCATTGGA 
GACTCCACAA CCATGATAAC CAGCCATCCA ACTGACATTC TTACTTCCTC AACTACAATC 
CGAATGTTCA TGCACGGTGC CGCACAGAGT CGCGTAGACA GTCTGGTCCT TGATATGCTT 
CTTCCAAAGC AAATGATTCT CCAACTCGTC AAGTCAATTT TGACAGAGAG ACGTCTGGTG 
TTAGCTGGAG CAACTGGAAT TGGAAAGAGC AAACTGGCGA AGACCCTGGC TGCTTATGTA 
TCTATTCGAA CAAATCAATC CGAAGATAGT ATTGTTAATA TCAGCATTCC TGAAAACAAT 
AAAGAAGAAT TGCTTCAAGT GGAACGACGC CTGGAAAAGA TCTTGAGAAG CAAAGAATCA 
TGCATCGTAA TTCTAGATAA TATCCCAAAG AATCGAATTG CATTTGTTGT ATCCGTTTTT 
GCAAATGTCC CACTTCAAAA CAACGAAGGT CCATTTGTAG TATGCACAGT CAACCGATAT 
CAAATCCCTG AGCTTCAAAT TCACCACAAT TTCAAAATGT CAGTAATGTC GAATCGTCTC 
GAAGGATTCA TCCTACGTTA CCTCCGACGA CGGGCGGTAG AGGATGAGTA TCGTCTAACT 
GTACAGATGC CATCAGAGCT CTTCAAAATC ATTGACTTCT TCCCAATAGC TCTTCAGGCC 
GTCAATAATT TTATTGAGAA AACGAATTCT GTTGATGTGA CAGTTGGTCC AAGAGCATGC 
TTGAACTGTC CTCTAACTGT CGATGGATCC CGTGAATGGT TCATTCGATT GTGGAATGAG 
AACTTCATTC CATATTTGGA ACGTGTTGCT AGAGATGGCA AAAAAACCTT CGGTCGCTGC 
ACTTCCTTCG AGGATCCCAC CGACATCGTC TCTGAAAAAT GGCCGTGGTT CGATGGTGAA 
AACCCGGAGA ATGTGCTCAA ACGTCTTCAA CTCCAAGACC TCGTCCCGTC ACCTGCCAAC 
TCATCCCGAC AACACTTCAA TCCCCTCGAG TCGTTGATCC AATTGCATGC TACCAAGCAT 
CAGACCATCG ACAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254273.1
CDS	1..4041
Translation	MLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267344.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267344.2

ORF Insert Sequence:

ATGCTGAAAT TAAAGTTATT CAGTAGCAAA AACCCATCTT CCTCATCGAA TAGCCCACAA 
CCTACGAGAA AGGCGGCGGC GGTGCCTCAA CAACAAACTT TGTCGAAAAT CGCTGCCCCA 
GTGAAAAGTG GCCTGAAGCC GCCGACCAGT AAGCTGGGAA GTGCCACGTC TATGTCGAAG 
CTTTGTACGC CAAAAGTTTC CTACCGTAAA ACGGACGCCC CAATCATATC TCAACAAGAC 
TCGAAACGAT GCTCAAAGAG CAGTGAAGAA GAGTCCGGAT ACGCTGGATT CAACAGCACG 
TCGCCAACGT CATCATCGAC GGAAGGTTCC CTAAGCATGC ATTCCACATC TTCCAAGAGT 
TCAACGTCAG ACGAAAAGTC TCCGTCATCA GACGATCTTA CTCTTAACGC CTCCATCGTG 
ACAGCTATCA GACAGCCGAT AGCCGCAACA CCGGTTTCTC CAAATATTAT CAACAAGCCT 
GTTGAGGAAA AACCAACACT GGCAGTGAAA GGAGTGAAAA GCACAGCGAA AAAAGATCCA 
CCTCCAGCTG TTCCGCCACG TGACACCCAG CCAACAATCG GAGTTGTTAG TCCAATTATG 
GCACATAAGA AGTTGACAAA TGACCCCGTG ATATCTGAAA AACCAGAACC TGAAAAGCTC 
CAATCAATGA GCATCGACAC GACGGACGTT CCACCGCTTC CACCTCTAAA ATCAGTTGTT 
CCACTTAAAA TGACTTCAAT CCGACAACCA CCAACGTACG ATGTTCTTCT AAAACAAGGA 
AAAATCACAT CGCCTGTCAA GTCGTTTGGA TATGAGCAGT CGTCCGCGTC TGAAGACTCC 
ATTGTGGCTC ATGCGTCGGC TCAGGTGACT CCGCCGACAA AAACTTCTGG TAATCATTCG 
CTGGAGAGAA GGATGGGAAA GAATAAGACA TCAGAATCCA GCGGCTACAC CTCTGACGCC 
GGTGTTGCGA TGTGCGCCAA AATGAGGGAG AAGCTGAAAG AATACGATGA CATGACTCGT 
CGAGCACAGA ACGGCTATCC TGACAACTTC GAAGACAGTT CCTCCTTGTC GTCTGGAATA 
TCCGATAACA ACGAGCTCGA CGACATATCC ACGGACGATT TGTCCGGAGT AGACATGGCA 
ACAGTCGCCT CCAAACATAG CGACTATTCC CACTTTGTTC GCCATCCCAC GTCTTCTTCC 
TCAAAGCCCC GAGTCCCCAG TCGGTCCTCC ACATCAGTCG ATTCTCGATC TCGAGCAGAA 
CAGGAGAATG TGTACAAACT TCTGTCCCAG TGCCGAACGA GCCAACGTGG CGCCGCTGCC 
ACCTCAACCT TCGGACAACA TTCGCTAAGA TCCCCGGGAT ACTCATCCTA TTCTCCACAC 
TTATCAGTGT CAGCTGATAA GGACACAATG TCTATGCACT CACAGACTAG TCGACGACCT 
TCTTCACAAA AACCAAGCTA TTCAGGCCAA TTTCATTCAC TTGATCGTAA ATGCCACCTT 
CAAGAGTTCA CATCCACCGA GCACAGAATG GCGGCTCTCT TGAGCCCGAG ACGGGTGCCG 
AACTCGATGT CGAAATATGA TTCTTCAGCA GCCGCCCTCA ACGCTTCGGG TATGTCTCGT 
TCAATGATCC TTCTCGAATC CCTTTCACCA CGACCACCTC GACGTCACCA ATCACCGGCT 
GACTCGTGCA TTATCACAGC TTCTCCATCA GCCCCACGAA GATCACATTC GCCACGTGGC 
CCCACCGCCC GTATCCCACT TTCTCTCGCT TCTTCGCCTG TCCACGTCAA TAACAATTGG 
GGATCCTACT CGGCGCGTTC CCGAGGTGGA AGCTCTACTG GTATCTATGG AGAGACGTTC 
CAACTGCACA GACTATCCGA TGAAAAATCC CCCGCACATT CTGCCAAAAG TGAGATGGGA 
TCCCAACTAT CACTGGCTAG CACGACAGCA TATGGATCTC TCAATGAGAA GTACGAACAT 
GCTATTCGGG ACATGGCACG TGACTTGGAG TGTTACAAGA ACACTGTCGA CTCACTAACC 
AAGAAACAGG AGAACTATGG AGCATTGTTT GATCTTTTTG AGCAAAAGCT TAGAAAACTC 
ACTCAACACA TTGATCGATC CAACTTGAAG CCTGAAGAGG CAATACGATT CAGGCAGGAC 
ATTGCTCATT TGAGGGATAT TAGCAATCAT CTTGCATCCA ACTCAGCTCA TGCTAACGAA 
GGCGCTGGTG AGCTTCTTCG TCAACCATCT CTGGAATCAG TTGCATCCCA TCGATCATCG 
ATGTCATCGT CGTCGAAAAG CAGCAAGCAG GAGAAGATCA GCTTGAGCTC GTTTGGCAAG 
AACAAGAAGA GCTGGATCCG CTCCTCACTC TCCAAGTTCA CCAAGAAGAA GAACAAGAAC 
TACGACGAAG CACATATGCC ATCAATTTCC GGATCTCAAG GAACTCTTGA CAACATTGAT 
GTGATTGAGT TGAAGCAAGA GCTCAAAGAA CGCGATAGTG CACTTTACGA AGTCCGCCTT 
GACAATCTGG ATCGTGCCCG CGAAGTTGAT GTTCTGAGGG AGACAGTGAA CAAGTTGAAA 
ACCGAGAACA AGCAATTAAA GAAAGAAGTG GACAAACTCA CCAACGGTCC AGCCACTCGT 
GCTTCTTCCC GCGCCTCAAT TCCAGTTATC TACGACGATG AGCATGTCTA TGATGCAGCG 
TGTAGCAGTA CATCAGCTAG TCAATCTTCG AAACGATCCT CTGGCTGCAA CTCAATCAAG 
GTTACTGTAA ACGTGGACAT CGCTGGAGAA ATCAGTTCGA TCGTTAACCC GGACAAAGAG 
ATAATCGTAG GATATCTTGC CATGCCAACC AGTCAGTCAT GCTGGAAAGA CATTGATGTT 
TCTATTCTAG GACTATTTGA AGTCTACCTA TCCAGAATTG ATGTGGAGCA TCAACTTGGA 
ATCGATGCTC GTGATTCTAT CCTTGGCTAT CAAATTGGTG AACTTCGACG CGTCATTGGA 
GACTCCACAA CCATGATAAC CAGCCATCCA ACTGACATTC TTACTTCCTC AACTACAATC 
CGAATGTTCA TGCACGGTGC CGCACAGAGT CGCGTAGACA GTCTGGTCCT TGATATGCTT 
CTTCCAAAGC AAATGATTCT CCAACTCGTC AAGTCAATTT TGACAGAGAG ACGTCTGGTG 
TTAGCTGGAG CAACTGGAAT TGGAAAGAGC AAACTGGCGA AGACCCTGGC TGCTTATGTA 
TCTATTCGAA CAAATCAATC CGAAGATAGT ATTGTTAATA TCAGCATTCC TGAAAACAAT 
AAAGAAGAAT TGCTTCAAGT GGAACGACGC CTGGAAAAGA TCTTGAGAAG CAAAGAATCA 
TGCATCGTAA TTCTAGATAA TATCCCAAAG AATCGAATTG CATTTGTTGT ATCCGTTTTT 
GCAAATGTCC CACTTCAAAA CAACGAAGGT CCATTTGTAG TATGCACAGT CAACCGATAT 
CAAATCCCTG AGCTTCAAAT TCACCACAAT TTCAAAATGT CAGTAATGTC GAATCGTCTC 
GAAGGATTCA TCCTACGTTA CCTCCGACGA CGGGCGGTAG AGGATGAGTA TCGTCTAACT 
GTACAGATGC CATCAGAGCT CTTCAAAATC ATTGACTTCT TCCCAATAGC TCTTCAGGCC 
GTCAATAATT TTATTGAGAA AACGAATTCT GTTGATGTGA CAGTTGGTCC AAGAGCATGC 
TTGAACTGTC CTCTAACTGT CGATGGATCC CGTGAATGGT TCATTCGATT GTGGAATGAG 
AACTTCATTC CATATTTGGA ACGTGTTGCT AGAGATGGCA AAAAAACCTT CGGTCGCTGC 
ACTTCCTTCG AGGATCCCAC CGACATCGTC TCTGAAAAAT GGCCGTGGTT CGATGGTGAA 
AACCCGGAGA ATGTGCTCAA ACGTCTTCAA CTCCAAGACC TCGTCCCGTC ACCTGCCAAC 
TCATCCCGAC AACACTTCAA TCCCCTCGAG TCGTTGATCC AATTGCATGC TACCAAGCAT 
CAGACCATCG ACAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267344.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254273.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa351863
Clone ID Related Accession (Same CDS sequence)	NM_001267344.2 , NM_001267344.1
Accession Version	NM_001267344.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGCTGAAAT TAAAGTTATT CAGTAGCAAA AACCCATCTT CCTCATCGAA TAGCCCACAA 
CCTACGAGAA AGGCGGCGGC GGTGCCTCAA CAACAAACTT TGTCGAAAAT CGCTGCCCCA 
GTGAAAAGTG GCCTGAAGCC GCCGACCAGT AAGCTGGGAA GTGCCACGTC TATGTCGAAG 
CTTTGTACGC CAAAAGTTTC CTACCGTAAA ACGGACGCCC CAATCATATC TCAACAAGAC 
TCGAAACGAT GCTCAAAGAG CAGTGAAGAA GAGTCCGGAT ACGCTGGATT CAACAGCACG 
TCGCCAACGT CATCATCGAC GGAAGGTTCC CTAAGCATGC ATTCCACATC TTCCAAGAGT 
TCAACGTCAG ACGAAAAGTC TCCGTCATCA GACGATCTTA CTCTTAACGC CTCCATCGTG 
ACAGCTATCA GACAGCCGAT AGCCGCAACA CCGGTTTCTC CAAATATTAT CAACAAGCCT 
GTTGAGGAAA AACCAACACT GGCAGTGAAA GGAGTGAAAA GCACAGCGAA AAAAGATCCA 
CCTCCAGCTG TTCCGCCACG TGACACCCAG CCAACAATCG GAGTTGTTAG TCCAATTATG 
GCACATAAGA AGTTGACAAA TGACCCCGTG ATATCTGAAA AACCAGAACC TGAAAAGCTC 
CAATCAATGA GCATCGACAC GACGGACGTT CCACCGCTTC CACCTCTAAA ATCAGTTGTT 
CCACTTAAAA TGACTTCAAT CCGACAACCA CCAACGTACG ATGTTCTTCT AAAACAAGGA 
AAAATCACAT CGCCTGTCAA GTCGTTTGGA TATGAGCAGT CGTCCGCGTC TGAAGACTCC 
ATTGTGGCTC ATGCGTCGGC TCAGGTGACT CCGCCGACAA AAACTTCTGG TAATCATTCG 
CTGGAGAGAA GGATGGGAAA GAATAAGACA TCAGAATCCA GCGGCTACAC CTCTGACGCC 
GGTGTTGCGA TGTGCGCCAA AATGAGGGAG AAGCTGAAAG AATACGATGA CATGACTCGT 
CGAGCACAGA ACGGCTATCC TGACAACTTC GAAGACAGTT CCTCCTTGTC GTCTGGAATA 
TCCGATAACA ACGAGCTCGA CGACATATCC ACGGACGATT TGTCCGGAGT AGACATGGCA 
ACAGTCGCCT CCAAACATAG CGACTATTCC CACTTTGTTC GCCATCCCAC GTCTTCTTCC 
TCAAAGCCCC GAGTCCCCAG TCGGTCCTCC ACATCAGTCG ATTCTCGATC TCGAGCAGAA 
CAGGAGAATG TGTACAAACT TCTGTCCCAG TGCCGAACGA GCCAACGTGG CGCCGCTGCC 
ACCTCAACCT TCGGACAACA TTCGCTAAGA TCCCCGGGAT ACTCATCCTA TTCTCCACAC 
TTATCAGTGT CAGCTGATAA GGACACAATG TCTATGCACT CACAGACTAG TCGACGACCT 
TCTTCACAAA AACCAAGCTA TTCAGGCCAA TTTCATTCAC TTGATCGTAA ATGCCACCTT 
CAAGAGTTCA CATCCACCGA GCACAGAATG GCGGCTCTCT TGAGCCCGAG ACGGGTGCCG 
AACTCGATGT CGAAATATGA TTCTTCAGCA GCCGCCCTCA ACGCTTCGGG TATGTCTCGT 
TCAATGATCC TTCTCGAATC CCTTTCACCA CGACCACCTC GACGTCACCA ATCACCGGCT 
GACTCGTGCA TTATCACAGC TTCTCCATCA GCCCCACGAA GATCACATTC GCCACGTGGC 
CCCACCGCCC GTATCCCACT TTCTCTCGCT TCTTCGCCTG TCCACGTCAA TAACAATTGG 
GGATCCTACT CGGCGCGTTC CCGAGGTGGA AGCTCTACTG GTATCTATGG AGAGACGTTC 
CAACTGCACA GACTATCCGA TGAAAAATCC CCCGCACATT CTGCCAAAAG TGAGATGGGA 
TCCCAACTAT CACTGGCTAG CACGACAGCA TATGGATCTC TCAATGAGAA GTACGAACAT 
GCTATTCGGG ACATGGCACG TGACTTGGAG TGTTACAAGA ACACTGTCGA CTCACTAACC 
AAGAAACAGG AGAACTATGG AGCATTGTTT GATCTTTTTG AGCAAAAGCT TAGAAAACTC 
ACTCAACACA TTGATCGATC CAACTTGAAG CCTGAAGAGG CAATACGATT CAGGCAGGAC 
ATTGCTCATT TGAGGGATAT TAGCAATCAT CTTGCATCCA ACTCAGCTCA TGCTAACGAA 
GGCGCTGGTG AGCTTCTTCG TCAACCATCT CTGGAATCAG TTGCATCCCA TCGATCATCG 
ATGTCATCGT CGTCGAAAAG CAGCAAGCAG GAGAAGATCA GCTTGAGCTC GTTTGGCAAG 
AACAAGAAGA GCTGGATCCG CTCCTCACTC TCCAAGTTCA CCAAGAAGAA GAACAAGAAC 
TACGACGAAG CACATATGCC ATCAATTTCC GGATCTCAAG GAACTCTTGA CAACATTGAT 
GTGATTGAGT TGAAGCAAGA GCTCAAAGAA CGCGATAGTG CACTTTACGA AGTCCGCCTT 
GACAATCTGG ATCGTGCCCG CGAAGTTGAT GTTCTGAGGG AGACAGTGAA CAAGTTGAAA 
ACCGAGAACA AGCAATTAAA GAAAGAAGTG GACAAACTCA CCAACGGTCC AGCCACTCGT 
GCTTCTTCCC GCGCCTCAAT TCCAGTTATC TACGACGATG AGCATGTCTA TGATGCAGCG 
TGTAGCAGTA CATCAGCTAG TCAATCTTCG AAACGATCCT CTGGCTGCAA CTCAATCAAG 
GTTACTGTAA ACGTGGACAT CGCTGGAGAA ATCAGTTCGA TCGTTAACCC GGACAAAGAG 
ATAATCGTAG GATATCTTGC CATGCCAACC AGTCAGTCAT GCTGGAAAGA CATTGATGTT 
TCTATTCTAG GACTATTTGA AGTCTACCTA TCCAGAATTG ATGTGGAGCA TCAACTTGGA 
ATCGATGCTC GTGATTCTAT CCTTGGCTAT CAAATTGGTG AACTTCGACG CGTCATTGGA 
GACTCCACAA CCATGATAAC CAGCCATCCA ACTGACATTC TTACTTCCTC AACTACAATC 
CGAATGTTCA TGCACGGTGC CGCACAGAGT CGCGTAGACA GTCTGGTCCT TGATATGCTT 
CTTCCAAAGC AAATGATTCT CCAACTCGTC AAGTCAATTT TGACAGAGAG ACGTCTGGTG 
TTAGCTGGAG CAACTGGAAT TGGAAAGAGC AAACTGGCGA AGACCCTGGC TGCTTATGTA 
TCTATTCGAA CAAATCAATC CGAAGATAGT ATTGTTAATA TCAGCATTCC TGAAAACAAT 
AAAGAAGAAT TGCTTCAAGT GGAACGACGC CTGGAAAAGA TCTTGAGAAG CAAAGAATCA 
TGCATCGTAA TTCTAGATAA TATCCCAAAG AATCGAATTG CATTTGTTGT ATCCGTTTTT 
GCAAATGTCC CACTTCAAAA CAACGAAGGT CCATTTGTAG TATGCACAGT CAACCGATAT 
CAAATCCCTG AGCTTCAAAT TCACCACAAT TTCAAAATGT CAGTAATGTC GAATCGTCTC 
GAAGGATTCA TCCTACGTTA CCTCCGACGA CGGGCGGTAG AGGATGAGTA TCGTCTAACT 
GTACAGATGC CATCAGAGCT CTTCAAAATC ATTGACTTCT TCCCAATAGC TCTTCAGGCC 
GTCAATAATT TTATTGAGAA AACGAATTCT GTTGATGTGA CAGTTGGTCC AAGAGCATGC 
TTGAACTGTC CTCTAACTGT CGATGGATCC CGTGAATGGT TCATTCGATT GTGGAATGAG 
AACTTCATTC CATATTTGGA ACGTGTTGCT AGAGATGGCA AAAAAACCTT CGGTCGCTGC 
ACTTCCTTCG AGGATCCCAC CGACATCGTC TCTGAAAAAT GGCCGTGGTT CGATGGTGAA 
AACCCGGAGA ATGTGCTCAA ACGTCTTCAA CTCCAAGACC TCGTCCCGTC ACCTGCCAAC 
TCATCCCGAC AACACTTCAA TCCCCTCGAG TCGTTGATCC AATTGCATGC TACCAAGCAT 
CAGACCATCG ACAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254273.1
CDS	1..4041
Translation	MLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267344.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267344.1

ORF Insert Sequence:

ATGCTGAAAT TAAAGTTATT CAGTAGCAAA AACCCATCTT CCTCATCGAA TAGCCCACAA 
CCTACGAGAA AGGCGGCGGC GGTGCCTCAA CAACAAACTT TGTCGAAAAT CGCTGCCCCA 
GTGAAAAGTG GCCTGAAGCC GCCGACCAGT AAGCTGGGAA GTGCCACGTC TATGTCGAAG 
CTTTGTACGC CAAAAGTTTC CTACCGTAAA ACGGACGCCC CAATCATATC TCAACAAGAC 
TCGAAACGAT GCTCAAAGAG CAGTGAAGAA GAGTCCGGAT ACGCTGGATT CAACAGCACG 
TCGCCAACGT CATCATCGAC GGAAGGTTCC CTAAGCATGC ATTCCACATC TTCCAAGAGT 
TCAACGTCAG ACGAAAAGTC TCCGTCATCA GACGATCTTA CTCTTAACGC CTCCATCGTG 
ACAGCTATCA GACAGCCGAT AGCCGCAACA CCGGTTTCTC CAAATATTAT CAACAAGCCT 
GTTGAGGAAA AACCAACACT GGCAGTGAAA GGAGTGAAAA GCACAGCGAA AAAAGATCCA 
CCTCCAGCTG TTCCGCCACG TGACACCCAG CCAACAATCG GAGTTGTTAG TCCAATTATG 
GCACATAAGA AGTTGACAAA TGACCCCGTG ATATCTGAAA AACCAGAACC TGAAAAGCTC 
CAATCAATGA GCATCGACAC GACGGACGTT CCACCGCTTC CACCTCTAAA ATCAGTTGTT 
CCACTTAAAA TGACTTCAAT CCGACAACCA CCAACGTACG ATGTTCTTCT AAAACAAGGA 
AAAATCACAT CGCCTGTCAA GTCGTTTGGA TATGAGCAGT CGTCCGCGTC TGAAGACTCC 
ATTGTGGCTC ATGCGTCGGC TCAGGTGACT CCGCCGACAA AAACTTCTGG TAATCATTCG 
CTGGAGAGAA GGATGGGAAA GAATAAGACA TCAGAATCCA GCGGCTACAC CTCTGACGCC 
GGTGTTGCGA TGTGCGCCAA AATGAGGGAG AAGCTGAAAG AATACGATGA CATGACTCGT 
CGAGCACAGA ACGGCTATCC TGACAACTTC GAAGACAGTT CCTCCTTGTC GTCTGGAATA 
TCCGATAACA ACGAGCTCGA CGACATATCC ACGGACGATT TGTCCGGAGT AGACATGGCA 
ACAGTCGCCT CCAAACATAG CGACTATTCC CACTTTGTTC GCCATCCCAC GTCTTCTTCC 
TCAAAGCCCC GAGTCCCCAG TCGGTCCTCC ACATCAGTCG ATTCTCGATC TCGAGCAGAA 
CAGGAGAATG TGTACAAACT TCTGTCCCAG TGCCGAACGA GCCAACGTGG CGCCGCTGCC 
ACCTCAACCT TCGGACAACA TTCGCTAAGA TCCCCGGGAT ACTCATCCTA TTCTCCACAC 
TTATCAGTGT CAGCTGATAA GGACACAATG TCTATGCACT CACAGACTAG TCGACGACCT 
TCTTCACAAA AACCAAGCTA TTCAGGCCAA TTTCATTCAC TTGATCGTAA ATGCCACCTT 
CAAGAGTTCA CATCCACCGA GCACAGAATG GCGGCTCTCT TGAGCCCGAG ACGGGTGCCG 
AACTCGATGT CGAAATATGA TTCTTCAGCA GCCGCCCTCA ACGCTTCGGG TATGTCTCGT 
TCAATGATCC TTCTCGAATC CCTTTCACCA CGACCACCTC GACGTCACCA ATCACCGGCT 
GACTCGTGCA TTATCACAGC TTCTCCATCA GCCCCACGAA GATCACATTC GCCACGTGGC 
CCCACCGCCC GTATCCCACT TTCTCTCGCT TCTTCGCCTG TCCACGTCAA TAACAATTGG 
GGATCCTACT CGGCGCGTTC CCGAGGTGGA AGCTCTACTG GTATCTATGG AGAGACGTTC 
CAACTGCACA GACTATCCGA TGAAAAATCC CCCGCACATT CTGCCAAAAG TGAGATGGGA 
TCCCAACTAT CACTGGCTAG CACGACAGCA TATGGATCTC TCAATGAGAA GTACGAACAT 
GCTATTCGGG ACATGGCACG TGACTTGGAG TGTTACAAGA ACACTGTCGA CTCACTAACC 
AAGAAACAGG AGAACTATGG AGCATTGTTT GATCTTTTTG AGCAAAAGCT TAGAAAACTC 
ACTCAACACA TTGATCGATC CAACTTGAAG CCTGAAGAGG CAATACGATT CAGGCAGGAC 
ATTGCTCATT TGAGGGATAT TAGCAATCAT CTTGCATCCA ACTCAGCTCA TGCTAACGAA 
GGCGCTGGTG AGCTTCTTCG TCAACCATCT CTGGAATCAG TTGCATCCCA TCGATCATCG 
ATGTCATCGT CGTCGAAAAG CAGCAAGCAG GAGAAGATCA GCTTGAGCTC GTTTGGCAAG 
AACAAGAAGA GCTGGATCCG CTCCTCACTC TCCAAGTTCA CCAAGAAGAA GAACAAGAAC 
TACGACGAAG CACATATGCC ATCAATTTCC GGATCTCAAG GAACTCTTGA CAACATTGAT 
GTGATTGAGT TGAAGCAAGA GCTCAAAGAA CGCGATAGTG CACTTTACGA AGTCCGCCTT 
GACAATCTGG ATCGTGCCCG CGAAGTTGAT GTTCTGAGGG AGACAGTGAA CAAGTTGAAA 
ACCGAGAACA AGCAATTAAA GAAAGAAGTG GACAAACTCA CCAACGGTCC AGCCACTCGT 
GCTTCTTCCC GCGCCTCAAT TCCAGTTATC TACGACGATG AGCATGTCTA TGATGCAGCG 
TGTAGCAGTA CATCAGCTAG TCAATCTTCG AAACGATCCT CTGGCTGCAA CTCAATCAAG 
GTTACTGTAA ACGTGGACAT CGCTGGAGAA ATCAGTTCGA TCGTTAACCC GGACAAAGAG 
ATAATCGTAG GATATCTTGC CATGCCAACC AGTCAGTCAT GCTGGAAAGA CATTGATGTT 
TCTATTCTAG GACTATTTGA AGTCTACCTA TCCAGAATTG ATGTGGAGCA TCAACTTGGA 
ATCGATGCTC GTGATTCTAT CCTTGGCTAT CAAATTGGTG AACTTCGACG CGTCATTGGA 
GACTCCACAA CCATGATAAC CAGCCATCCA ACTGACATTC TTACTTCCTC AACTACAATC 
CGAATGTTCA TGCACGGTGC CGCACAGAGT CGCGTAGACA GTCTGGTCCT TGATATGCTT 
CTTCCAAAGC AAATGATTCT CCAACTCGTC AAGTCAATTT TGACAGAGAG ACGTCTGGTG 
TTAGCTGGAG CAACTGGAAT TGGAAAGAGC AAACTGGCGA AGACCCTGGC TGCTTATGTA 
TCTATTCGAA CAAATCAATC CGAAGATAGT ATTGTTAATA TCAGCATTCC TGAAAACAAT 
AAAGAAGAAT TGCTTCAAGT GGAACGACGC CTGGAAAAGA TCTTGAGAAG CAAAGAATCA 
TGCATCGTAA TTCTAGATAA TATCCCAAAG AATCGAATTG CATTTGTTGT ATCCGTTTTT 
GCAAATGTCC CACTTCAAAA CAACGAAGGT CCATTTGTAG TATGCACAGT CAACCGATAT 
CAAATCCCTG AGCTTCAAAT TCACCACAAT TTCAAAATGT CAGTAATGTC GAATCGTCTC 
GAAGGATTCA TCCTACGTTA CCTCCGACGA CGGGCGGTAG AGGATGAGTA TCGTCTAACT 
GTACAGATGC CATCAGAGCT CTTCAAAATC ATTGACTTCT TCCCAATAGC TCTTCAGGCC 
GTCAATAATT TTATTGAGAA AACGAATTCT GTTGATGTGA CAGTTGGTCC AAGAGCATGC 
TTGAACTGTC CTCTAACTGT CGATGGATCC CGTGAATGGT TCATTCGATT GTGGAATGAG 
AACTTCATTC CATATTTGGA ACGTGTTGCT AGAGATGGCA AAAAAACCTT CGGTCGCTGC 
ACTTCCTTCG AGGATCCCAC CGACATCGTC TCTGAAAAAT GGCCGTGGTT CGATGGTGAA 
AACCCGGAGA ATGTGCTCAA ACGTCTTCAA CTCCAAGACC TCGTCCCGTC ACCTGCCAAC 
TCATCCCGAC AACACTTCAA TCCCCTCGAG TCGTTGATCC AATTGCATGC TACCAAGCAT 
CAGACCATCG ACAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001026999.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022170.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Price & Availability↑

CloneID	OCa337678
Clone ID Related Accession (Same CDS sequence)	NM_001026999.5 , NM_001026999.6
Accession Version	NM_001026999.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001026999.4. COMPLETENESS: incomplete on both ends.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022170.1
CDS	1..4965
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001026999.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026999.5

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001026999.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022170.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

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CloneID	OCa337678
Clone ID Related Accession (Same CDS sequence)	NM_001026999.5 , NM_001026999.6
Accession Version	NM_001026999.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001026999.5.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022170.1
CDS	42..5006
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001026999.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026999.6

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGCAGCCGCC 
CTCAACGCTT CGGGTATGTC TCGTTCAATG ATCCTTCTCG AATCCCTTTC ACCACGACCA 
CCTCGACGTC ACCAATCACC GGCTGACTCG TGCATTATCA CAGCTTCTCC ATCAGCCCCA 
CGAAGATCAC ATTCGCCACG TGGCCCCACC GCCCGTATCC CACTTTCTCT CGCTTCTTCG 
CCTGTCCACG TCAATAACAA TTGGGGATCC TACTCGGCGC GTTCCCGAGG TGGAAGCTCT 
ACTGGTATCT ATGGAGAGAC GTTCCAACTG CACAGACTAT CCGATGAAAA ATCCCCCGCA 
CATTCTGCCA AAAGTGAGAT GGGATCCCAA CTATCACTGG CTAGCACGAC AGCATATGGA 
TCTCTCAATG AGAAGTACGA ACATGCTATT CGGGACATGG CACGTGACTT GGAGTGTTAC 
AAGAACACTG TCGACTCACT AACCAAGAAA CAGGAGAACT ATGGAGCATT GTTTGATCTT 
TTTGAGCAAA AGCTTAGAAA ACTCACTCAA CACATTGATC GATCCAACTT GAAGCCTGAA 
GAGGCAATAC GATTCAGGCA GGACATTGCT CATTTGAGGG ATATTAGCAA TCATCTTGCA 
TCCAACTCAG CTCATGCTAA CGAAGGCGCT GGTGAGCTTC TTCGTCAACC ATCTCTGGAA 
TCAGTTGCAT CCCATCGATC ATCGATGTCA TCGTCGTCGA AAAGCAGCAA GCAGGAGAAG 
ATCAGCTTGA GCTCGTTTGG CAAGAACAAG AAGAGCTGGA TCCGCTCCTC ACTCTCCAAG 
TTCACCAAGA AGAAGAACAA GAACTACGAC GAAGCACATA TGCCATCAAT TTCCGGATCT 
CAAGGAACTC TTGACAACAT TGATGTGATT GAGTTGAAGC AAGAGCTCAA AGAACGCGAT 
AGTGCACTTT ACGAAGTCCG CCTTGACAAT CTGGATCGTG CCCGCGAAGT TGATGTTCTG 
AGGGAGACAG TGAACAAGTT GAAAACCGAG AACAAGCAAT TAAAGAAAGA AGTGGACAAA 
CTCACCAACG GTCCAGCCAC TCGTGCTTCT TCCCGCGCCT CAATTCCAGT TATCTACGAC 
GATGAGCATG TCTATGATGC AGCGTGTAGC AGTACATCAG CTAGTCAATC TTCGAAACGA 
TCCTCTGGCT GCAACTCAAT CAAGGTTACT GTAAACGTGG ACATCGCTGG AGAAATCAGT 
TCGATCGTTA ACCCGGACAA AGAGATAATC GTAGGATATC TTGCCATGCC AACCAGTCAG 
TCATGCTGGA AAGACATTGA TGTTTCTATT CTAGGACTAT TTGAAGTCTA CCTATCCAGA 
ATTGATGTGG AGCATCAACT TGGAATCGAT GCTCGTGATT CTATCCTTGG CTATCAAATT 
GGTGAACTTC GACGCGTCAT TGGAGACTCC ACAACCATGA TAACCAGCCA TCCAACTGAC 
ATTCTTACTT CCTCAACTAC AATCCGAATG TTCATGCACG GTGCCGCACA GAGTCGCGTA 
GACAGTCTGG TCCTTGATAT GCTTCTTCCA AAGCAAATGA TTCTCCAACT CGTCAAGTCA 
ATTTTGACAG AGAGACGTCT GGTGTTAGCT GGAGCAACTG GAATTGGAAA GAGCAAACTG 
GCGAAGACCC TGGCTGCTTA TGTATCTATT CGAACAAATC AATCCGAAGA TAGTATTGTT 
AATATCAGCA TTCCTGAAAA CAATAAAGAA GAATTGCTTC AAGTGGAACG ACGCCTGGAA 
AAGATCTTGA GAAGCAAAGA ATCATGCATC GTAATTCTAG ATAATATCCC AAAGAATCGA 
ATTGCATTTG TTGTATCCGT TTTTGCAAAT GTCCCACTTC AAAACAACGA AGGTCCATTT 
GTAGTATGCA CAGTCAACCG ATATCAAATC CCTGAGCTTC AAATTCACCA CAATTTCAAA 
ATGTCAGTAA TGTCGAATCG TCTCGAAGGA TTCATCCTAC GTTACCTCCG ACGACGGGCG 
GTAGAGGATG AGTATCGTCT AACTGTACAG ATGCCATCAG AGCTCTTCAA AATCATTGAC 
TTCTTCCCAA TAGCTCTTCA GGCCGTCAAT AATTTTATTG AGAAAACGAA TTCTGTTGAT 
GTGACAGTTG GTCCAAGAGC ATGCTTGAAC TGTCCTCTAA CTGTCGATGG ATCCCGTGAA 
TGGTTCATTC GATTGTGGAA TGAGAACTTC ATTCCATATT TGGAACGTGT TGCTAGAGAT 
GGCAAAAAAA CCTTCGGTCG CTGCACTTCC TTCGAGGATC CCACCGACAT CGTCTCTGAA 
AAATGGCCGT GGTTCGATGG TGAAAACCCG GAGAATGTGC TCAAACGTCT TCAACTCCAA 
GACCTCGTCC CGTCACCTGC CAACTCATCC CGACAACACT TCAATCCCCT CGAGTCGTTG 
ATCCAATTGC ATGCTACCAA GCATCAGACC ATCGACAACA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_171039.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741045.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa338199
Clone ID Related Accession (Same CDS sequence)	NM_171039.2 , NM_171039.3
Accession Version	NM_171039.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_171039.1. COMPLETENESS: incomplete on both ends.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGGATCCTAC 
TCGGCGCGTT CCCGAGGTGG AAGCTCTACT GGTATCTATG GAGAGACGTT CCAACTGCAC 
AGACTATCCG ATGAAAAATC CCCCGCACAT TCTGCCAAAA GTGAGATGGG ATCCCAACTA 
TCACTGGCTA GCACGACAGC ATATGGATCT CTCAATGAGA AGTACGAACA TGCTATTCGG 
GACATGGCAC GTGACTTGGA GTGTTACAAG AACACTGTCG ACTCACTAAC CAAGAAACAG 
GAGAACTATG GAGCATTGTT TGATCTTTTT GAGCAAAAGC TTAGAAAACT CACTCAACAC 
ATTGATCGAT CCAACTTGAA GCCTGAAGAG GCAATACGAT TCAGGCAGGA CATTGCTCAT 
TTGAGGGATA TTAGCAATCA TCTTGCATCC AACTCAGCTC ATGCTAACGA AGGCGCTGGT 
GAGCTTCTTC GTCAACCATC TCTGGAATCA GTTGCATCCC ATCGATCATC GATGTCATCG 
TCGTCGAAAA GCAGCAAGCA GGAGAAGATC AGCTTGAGCT CGTTTGGCAA GAACAAGAAG 
AGCTGGATCC GCTCCTCACT CTCCAAGTTC ACCAAGAAGA AGAACAAGAA CTACGACGAA 
GCACATATGC CATCAATTTC CGGATCTCAA GGAACTCTTG ACAACATTGA TGTGATTGAG 
TTGAAGCAAG AGCTCAAAGA ACGCGATAGT GCACTTTACG AAGTCCGCCT TGACAATCTG 
GATCGTGCCC GCGAAGTTGA TGTTCTGAGG GAGACAGTGA ACAAGTTGAA AACCGAGAAC 
AAGCAATTAA AGAAAGAAGT GGACAAACTC ACCAACGGTC CAGCCACTCG TGCTTCTTCC 
CGCGCCTCAA TTCCAGTTAT CTACGACGAT GAGCATGTCT ATGATGCAGC GTGTAGCAGT 
ACATCAGCTA GTCAATCTTC GAAACGATCC TCTGGCTGCA ACTCAATCAA GGTTACTGTA 
AACGTGGACA TCGCTGGAGA AATCAGTTCG ATCGTTAACC CGGACAAAGA GATAATCGTA 
GGATATCTTG CCATGCCAAC CAGTCAGTCA TGCTGGAAAG ACATTGATGT TTCTATTCTA 
GGACTATTTG AAGTCTACCT ATCCAGAATT GATGTGGAGC ATCAACTTGG AATCGATGCT 
CGTGATTCTA TCCTTGGCTA TCAAATTGGT GAACTTCGAC GCGTCATTGG AGACTCCACA 
ACCATGATAA CCAGCCATCC AACTGACATT CTTACTTCCT CAACTACAAT CCGAATGTTC 
ATGCACGGTG CCGCACAGAG TCGCGTAGAC AGTCTGGTCC TTGATATGCT TCTTCCAAAG 
CAAATGATTC TCCAACTCGT CAAGTCAATT TTGACAGAGA GACGTCTGGT GTTAGCTGGA 
GCAACTGGAA TTGGAAAGAG CAAACTGGCG AAGACCCTGG CTGCTTATGT ATCTATTCGA 
ACAAATCAAT CCGAAGATAG TATTGTTAAT ATCAGCATTC CTGAAAACAA TAAAGAAGAA 
TTGCTTCAAG TGGAACGACG CCTGGAAAAG ATCTTGAGAA GCAAAGAATC ATGCATCGTA 
ATTCTAGATA ATATCCCAAA GAATCGAATT GCATTTGTTG TATCCGTTTT TGCAAATGTC 
CCACTTCAAA ACAACGAAGG TCCATTTGTA GTATGCACAG TCAACCGATA TCAAATCCCT 
GAGCTTCAAA TTCACCACAA TTTCAAAATG TCAGTAATGT CGAATCGTCT CGAAGGATTC 
ATCCTACGTT ACCTCCGACG ACGGGCGGTA GAGGATGAGT ATCGTCTAAC TGTACAGATG 
CCATCAGAGC TCTTCAAAAT CATTGACTTC TTCCCAATAG CTCTTCAGGC CGTCAATAAT 
TTTATTGAGA AAACGAATTC TGTTGATGTG ACAGTTGGTC CAAGAGCATG CTTGAACTGT 
CCTCTAACTG TCGATGGATC CCGTGAATGG TTCATTCGAT TGTGGAATGA GAACTTCATT 
CCATATTTGG AACGTGTTGC TAGAGATGGC AAAAAAACCT TCGGTCGCTG CACTTCCTTC 
GAGGATCCCA CCGACATCGT CTCTGAAAAA TGGCCGTGGT TCGATGGTGA AAACCCGGAG 
AATGTGCTCA AACGTCTTCA ACTCCAAGAC CTCGTCCCGT CACCTGCCAA CTCATCCCGA 
CAACACTTCA ATCCCCTCGA GTCGTTGATC CAATTGCATG CTACCAAGCA TCAGACCATC 
GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741045.1
CDS	1..4752
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_171039.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171039.2

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGGATCCTAC 
TCGGCGCGTT CCCGAGGTGG AAGCTCTACT GGTATCTATG GAGAGACGTT CCAACTGCAC 
AGACTATCCG ATGAAAAATC CCCCGCACAT TCTGCCAAAA GTGAGATGGG ATCCCAACTA 
TCACTGGCTA GCACGACAGC ATATGGATCT CTCAATGAGA AGTACGAACA TGCTATTCGG 
GACATGGCAC GTGACTTGGA GTGTTACAAG AACACTGTCG ACTCACTAAC CAAGAAACAG 
GAGAACTATG GAGCATTGTT TGATCTTTTT GAGCAAAAGC TTAGAAAACT CACTCAACAC 
ATTGATCGAT CCAACTTGAA GCCTGAAGAG GCAATACGAT TCAGGCAGGA CATTGCTCAT 
TTGAGGGATA TTAGCAATCA TCTTGCATCC AACTCAGCTC ATGCTAACGA AGGCGCTGGT 
GAGCTTCTTC GTCAACCATC TCTGGAATCA GTTGCATCCC ATCGATCATC GATGTCATCG 
TCGTCGAAAA GCAGCAAGCA GGAGAAGATC AGCTTGAGCT CGTTTGGCAA GAACAAGAAG 
AGCTGGATCC GCTCCTCACT CTCCAAGTTC ACCAAGAAGA AGAACAAGAA CTACGACGAA 
GCACATATGC CATCAATTTC CGGATCTCAA GGAACTCTTG ACAACATTGA TGTGATTGAG 
TTGAAGCAAG AGCTCAAAGA ACGCGATAGT GCACTTTACG AAGTCCGCCT TGACAATCTG 
GATCGTGCCC GCGAAGTTGA TGTTCTGAGG GAGACAGTGA ACAAGTTGAA AACCGAGAAC 
AAGCAATTAA AGAAAGAAGT GGACAAACTC ACCAACGGTC CAGCCACTCG TGCTTCTTCC 
CGCGCCTCAA TTCCAGTTAT CTACGACGAT GAGCATGTCT ATGATGCAGC GTGTAGCAGT 
ACATCAGCTA GTCAATCTTC GAAACGATCC TCTGGCTGCA ACTCAATCAA GGTTACTGTA 
AACGTGGACA TCGCTGGAGA AATCAGTTCG ATCGTTAACC CGGACAAAGA GATAATCGTA 
GGATATCTTG CCATGCCAAC CAGTCAGTCA TGCTGGAAAG ACATTGATGT TTCTATTCTA 
GGACTATTTG AAGTCTACCT ATCCAGAATT GATGTGGAGC ATCAACTTGG AATCGATGCT 
CGTGATTCTA TCCTTGGCTA TCAAATTGGT GAACTTCGAC GCGTCATTGG AGACTCCACA 
ACCATGATAA CCAGCCATCC AACTGACATT CTTACTTCCT CAACTACAAT CCGAATGTTC 
ATGCACGGTG CCGCACAGAG TCGCGTAGAC AGTCTGGTCC TTGATATGCT TCTTCCAAAG 
CAAATGATTC TCCAACTCGT CAAGTCAATT TTGACAGAGA GACGTCTGGT GTTAGCTGGA 
GCAACTGGAA TTGGAAAGAG CAAACTGGCG AAGACCCTGG CTGCTTATGT ATCTATTCGA 
ACAAATCAAT CCGAAGATAG TATTGTTAAT ATCAGCATTC CTGAAAACAA TAAAGAAGAA 
TTGCTTCAAG TGGAACGACG CCTGGAAAAG ATCTTGAGAA GCAAAGAATC ATGCATCGTA 
ATTCTAGATA ATATCCCAAA GAATCGAATT GCATTTGTTG TATCCGTTTT TGCAAATGTC 
CCACTTCAAA ACAACGAAGG TCCATTTGTA GTATGCACAG TCAACCGATA TCAAATCCCT 
GAGCTTCAAA TTCACCACAA TTTCAAAATG TCAGTAATGT CGAATCGTCT CGAAGGATTC 
ATCCTACGTT ACCTCCGACG ACGGGCGGTA GAGGATGAGT ATCGTCTAAC TGTACAGATG 
CCATCAGAGC TCTTCAAAAT CATTGACTTC TTCCCAATAG CTCTTCAGGC CGTCAATAAT 
TTTATTGAGA AAACGAATTC TGTTGATGTG ACAGTTGGTC CAAGAGCATG CTTGAACTGT 
CCTCTAACTG TCGATGGATC CCGTGAATGG TTCATTCGAT TGTGGAATGA GAACTTCATT 
CCATATTTGG AACGTGTTGC TAGAGATGGC AAAAAAACCT TCGGTCGCTG CACTTCCTTC 
GAGGATCCCA CCGACATCGT CTCTGAAAAA TGGCCGTGGT TCGATGGTGA AAACCCGGAG 
AATGTGCTCA AACGTCTTCA ACTCCAAGAC CTCGTCCCGT CACCTGCCAA CTCATCCCGA 
CAACACTTCA ATCCCCTCGA GTCGTTGATC CAATTGCATG CTACCAAGCA TCAGACCATC 
GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_171039.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741045.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

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CloneID	OCa338199
Clone ID Related Accession (Same CDS sequence)	NM_171039.2 , NM_171039.3
Accession Version	NM_171039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_171039.2.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGGATCCTAC 
TCGGCGCGTT CCCGAGGTGG AAGCTCTACT GGTATCTATG GAGAGACGTT CCAACTGCAC 
AGACTATCCG ATGAAAAATC CCCCGCACAT TCTGCCAAAA GTGAGATGGG ATCCCAACTA 
TCACTGGCTA GCACGACAGC ATATGGATCT CTCAATGAGA AGTACGAACA TGCTATTCGG 
GACATGGCAC GTGACTTGGA GTGTTACAAG AACACTGTCG ACTCACTAAC CAAGAAACAG 
GAGAACTATG GAGCATTGTT TGATCTTTTT GAGCAAAAGC TTAGAAAACT CACTCAACAC 
ATTGATCGAT CCAACTTGAA GCCTGAAGAG GCAATACGAT TCAGGCAGGA CATTGCTCAT 
TTGAGGGATA TTAGCAATCA TCTTGCATCC AACTCAGCTC ATGCTAACGA AGGCGCTGGT 
GAGCTTCTTC GTCAACCATC TCTGGAATCA GTTGCATCCC ATCGATCATC GATGTCATCG 
TCGTCGAAAA GCAGCAAGCA GGAGAAGATC AGCTTGAGCT CGTTTGGCAA GAACAAGAAG 
AGCTGGATCC GCTCCTCACT CTCCAAGTTC ACCAAGAAGA AGAACAAGAA CTACGACGAA 
GCACATATGC CATCAATTTC CGGATCTCAA GGAACTCTTG ACAACATTGA TGTGATTGAG 
TTGAAGCAAG AGCTCAAAGA ACGCGATAGT GCACTTTACG AAGTCCGCCT TGACAATCTG 
GATCGTGCCC GCGAAGTTGA TGTTCTGAGG GAGACAGTGA ACAAGTTGAA AACCGAGAAC 
AAGCAATTAA AGAAAGAAGT GGACAAACTC ACCAACGGTC CAGCCACTCG TGCTTCTTCC 
CGCGCCTCAA TTCCAGTTAT CTACGACGAT GAGCATGTCT ATGATGCAGC GTGTAGCAGT 
ACATCAGCTA GTCAATCTTC GAAACGATCC TCTGGCTGCA ACTCAATCAA GGTTACTGTA 
AACGTGGACA TCGCTGGAGA AATCAGTTCG ATCGTTAACC CGGACAAAGA GATAATCGTA 
GGATATCTTG CCATGCCAAC CAGTCAGTCA TGCTGGAAAG ACATTGATGT TTCTATTCTA 
GGACTATTTG AAGTCTACCT ATCCAGAATT GATGTGGAGC ATCAACTTGG AATCGATGCT 
CGTGATTCTA TCCTTGGCTA TCAAATTGGT GAACTTCGAC GCGTCATTGG AGACTCCACA 
ACCATGATAA CCAGCCATCC AACTGACATT CTTACTTCCT CAACTACAAT CCGAATGTTC 
ATGCACGGTG CCGCACAGAG TCGCGTAGAC AGTCTGGTCC TTGATATGCT TCTTCCAAAG 
CAAATGATTC TCCAACTCGT CAAGTCAATT TTGACAGAGA GACGTCTGGT GTTAGCTGGA 
GCAACTGGAA TTGGAAAGAG CAAACTGGCG AAGACCCTGG CTGCTTATGT ATCTATTCGA 
ACAAATCAAT CCGAAGATAG TATTGTTAAT ATCAGCATTC CTGAAAACAA TAAAGAAGAA 
TTGCTTCAAG TGGAACGACG CCTGGAAAAG ATCTTGAGAA GCAAAGAATC ATGCATCGTA 
ATTCTAGATA ATATCCCAAA GAATCGAATT GCATTTGTTG TATCCGTTTT TGCAAATGTC 
CCACTTCAAA ACAACGAAGG TCCATTTGTA GTATGCACAG TCAACCGATA TCAAATCCCT 
GAGCTTCAAA TTCACCACAA TTTCAAAATG TCAGTAATGT CGAATCGTCT CGAAGGATTC 
ATCCTACGTT ACCTCCGACG ACGGGCGGTA GAGGATGAGT ATCGTCTAAC TGTACAGATG 
CCATCAGAGC TCTTCAAAAT CATTGACTTC TTCCCAATAG CTCTTCAGGC CGTCAATAAT 
TTTATTGAGA AAACGAATTC TGTTGATGTG ACAGTTGGTC CAAGAGCATG CTTGAACTGT 
CCTCTAACTG TCGATGGATC CCGTGAATGG TTCATTCGAT TGTGGAATGA GAACTTCATT 
CCATATTTGG AACGTGTTGC TAGAGATGGC AAAAAAACCT TCGGTCGCTG CACTTCCTTC 
GAGGATCCCA CCGACATCGT CTCTGAAAAA TGGCCGTGGT TCGATGGTGA AAACCCGGAG 
AATGTGCTCA AACGTCTTCA ACTCCAAGAC CTCGTCCCGT CACCTGCCAA CTCATCCCGA 
CAACACTTCA ATCCCCTCGA GTCGTTGATC CAATTGCATG CTACCAAGCA TCAGACCATC 
GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741045.1
CDS	42..4793
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_171039.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171039.3

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG CCAATTTCAT 
TCACTTGATC GTAAATGCCA CCTTCAAGAG TTCACATCCA CCGAGCACAG AATGGCGGCT 
CTCTTGAGCC CGAGACGGGT GCCGAACTCG ATGTCGAAAT ATGATTCTTC AGGATCCTAC 
TCGGCGCGTT CCCGAGGTGG AAGCTCTACT GGTATCTATG GAGAGACGTT CCAACTGCAC 
AGACTATCCG ATGAAAAATC CCCCGCACAT TCTGCCAAAA GTGAGATGGG ATCCCAACTA 
TCACTGGCTA GCACGACAGC ATATGGATCT CTCAATGAGA AGTACGAACA TGCTATTCGG 
GACATGGCAC GTGACTTGGA GTGTTACAAG AACACTGTCG ACTCACTAAC CAAGAAACAG 
GAGAACTATG GAGCATTGTT TGATCTTTTT GAGCAAAAGC TTAGAAAACT CACTCAACAC 
ATTGATCGAT CCAACTTGAA GCCTGAAGAG GCAATACGAT TCAGGCAGGA CATTGCTCAT 
TTGAGGGATA TTAGCAATCA TCTTGCATCC AACTCAGCTC ATGCTAACGA AGGCGCTGGT 
GAGCTTCTTC GTCAACCATC TCTGGAATCA GTTGCATCCC ATCGATCATC GATGTCATCG 
TCGTCGAAAA GCAGCAAGCA GGAGAAGATC AGCTTGAGCT CGTTTGGCAA GAACAAGAAG 
AGCTGGATCC GCTCCTCACT CTCCAAGTTC ACCAAGAAGA AGAACAAGAA CTACGACGAA 
GCACATATGC CATCAATTTC CGGATCTCAA GGAACTCTTG ACAACATTGA TGTGATTGAG 
TTGAAGCAAG AGCTCAAAGA ACGCGATAGT GCACTTTACG AAGTCCGCCT TGACAATCTG 
GATCGTGCCC GCGAAGTTGA TGTTCTGAGG GAGACAGTGA ACAAGTTGAA AACCGAGAAC 
AAGCAATTAA AGAAAGAAGT GGACAAACTC ACCAACGGTC CAGCCACTCG TGCTTCTTCC 
CGCGCCTCAA TTCCAGTTAT CTACGACGAT GAGCATGTCT ATGATGCAGC GTGTAGCAGT 
ACATCAGCTA GTCAATCTTC GAAACGATCC TCTGGCTGCA ACTCAATCAA GGTTACTGTA 
AACGTGGACA TCGCTGGAGA AATCAGTTCG ATCGTTAACC CGGACAAAGA GATAATCGTA 
GGATATCTTG CCATGCCAAC CAGTCAGTCA TGCTGGAAAG ACATTGATGT TTCTATTCTA 
GGACTATTTG AAGTCTACCT ATCCAGAATT GATGTGGAGC ATCAACTTGG AATCGATGCT 
CGTGATTCTA TCCTTGGCTA TCAAATTGGT GAACTTCGAC GCGTCATTGG AGACTCCACA 
ACCATGATAA CCAGCCATCC AACTGACATT CTTACTTCCT CAACTACAAT CCGAATGTTC 
ATGCACGGTG CCGCACAGAG TCGCGTAGAC AGTCTGGTCC TTGATATGCT TCTTCCAAAG 
CAAATGATTC TCCAACTCGT CAAGTCAATT TTGACAGAGA GACGTCTGGT GTTAGCTGGA 
GCAACTGGAA TTGGAAAGAG CAAACTGGCG AAGACCCTGG CTGCTTATGT ATCTATTCGA 
ACAAATCAAT CCGAAGATAG TATTGTTAAT ATCAGCATTC CTGAAAACAA TAAAGAAGAA 
TTGCTTCAAG TGGAACGACG CCTGGAAAAG ATCTTGAGAA GCAAAGAATC ATGCATCGTA 
ATTCTAGATA ATATCCCAAA GAATCGAATT GCATTTGTTG TATCCGTTTT TGCAAATGTC 
CCACTTCAAA ACAACGAAGG TCCATTTGTA GTATGCACAG TCAACCGATA TCAAATCCCT 
GAGCTTCAAA TTCACCACAA TTTCAAAATG TCAGTAATGT CGAATCGTCT CGAAGGATTC 
ATCCTACGTT ACCTCCGACG ACGGGCGGTA GAGGATGAGT ATCGTCTAAC TGTACAGATG 
CCATCAGAGC TCTTCAAAAT CATTGACTTC TTCCCAATAG CTCTTCAGGC CGTCAATAAT 
TTTATTGAGA AAACGAATTC TGTTGATGTG ACAGTTGGTC CAAGAGCATG CTTGAACTGT 
CCTCTAACTG TCGATGGATC CCGTGAATGG TTCATTCGAT TGTGGAATGA GAACTTCATT 
CCATATTTGG AACGTGTTGC TAGAGATGGC AAAAAAACCT TCGGTCGCTG CACTTCCTTC 
GAGGATCCCA CCGACATCGT CTCTGAAAAA TGGCCGTGGT TCGATGGTGA AAACCCGGAG 
AATGTGCTCA AACGTCTTCA ACTCCAAGAC CTCGTCCCGT CACCTGCCAA CTCATCCCGA 
CAACACTTCA ATCCCCTCGA GTCGTTGATC CAATTGCATG CTACCAAGCA TCAGACCATC 
GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001026998.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022169.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

Price & Availability↑

CloneID	OCa338200
Clone ID Related Accession (Same CDS sequence)	NM_001026998.4 , NM_001026998.3
Accession Version	NM_001026998.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001026998.3.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGGCGCTTT CCGTAGATTC TCAGATCCGC TCCTCACTCT CCAAGTTCAC CAAGAAGAAG 
AACAAGAACT ACGACGAAGC ACATATGCCA TCAATTTCCG GATCTCAAGG AACTCTTGAC 
AACATTGATG TGATTGAGTT GAAGCAAGAG CTCAAAGAAC GCGATAGTGC ACTTTACGAA 
GTCCGCCTTG ACAATCTGGA TCGTGCCCGC GAAGTTGATG TTCTGAGGGA GACAGTGAAC 
AAGTTGAAAA CCGAGAACAA GCAATTAAAG AAAGAAGTGG ACAAACTCAC CAACGGTCCA 
GCCACTCGTG CTTCTTCCCG CGCCTCAATT CCAGTTATCT ACGACGATGA GCATGTCTAT 
GATGCAGCGT GTAGCAGTAC ATCAGCTAGT CAATCTTCGA AACGATCCTC TGGCTGCAAC 
TCAATCAAGG TTACTGTAAA CGTGGACATC GCTGGAGAAA TCAGTTCGAT CGTTAACCCG 
GACAAAGAGA TAATCGTAGG ATATCTTGCC ATGCCAACCA GTCAGTCATG CTGGAAAGAC 
ATTGATGTTT CTATTCTAGG ACTATTTGAA GTCTACCTAT CCAGAATTGA TGTGGAGCAT 
CAACTTGGAA TCGATGCTCG TGATTCTATC CTTGGCTATC AAATTGGTGA ACTTCGACGC 
GTCATTGGAG ACTCCACAAC CATGATAACC AGCCATCCAA CTGACATTCT TACTTCCTCA 
ACTACAATCC GAATGTTCAT GCACGGTGCC GCACAGAGTC GCGTAGACAG TCTGGTCCTT 
GATATGCTTC TTCCAAAGCA AATGATTCTC CAACTCGTCA AGTCAATTTT GACAGAGAGA 
CGTCTGGTGT TAGCTGGAGC AACTGGAATT GGAAAGAGCA AACTGGCGAA GACCCTGGCT 
GCTTATGTAT CTATTCGAAC AAATCAATCC GAAGATAGTA TTGTTAATAT CAGCATTCCT 
GAAAACAATA AAGAAGAATT GCTTCAAGTG GAACGACGCC TGGAAAAGAT CTTGAGAAGC 
AAAGAATCAT GCATCGTAAT TCTAGATAAT ATCCCAAAGA ATCGAATTGC ATTTGTTGTA 
TCCGTTTTTG CAAATGTCCC ACTTCAAAAC AACGAAGGTC CATTTGTAGT ATGCACAGTC 
AACCGATATC AAATCCCTGA GCTTCAAATT CACCACAATT TCAAAATGTC AGTAATGTCG 
AATCGTCTCG AAGGATTCAT CCTACGTTAC CTCCGACGAC GGGCGGTAGA GGATGAGTAT 
CGTCTAACTG TACAGATGCC ATCAGAGCTC TTCAAAATCA TTGACTTCTT CCCAATAGCT 
CTTCAGGCCG TCAATAATTT TATTGAGAAA ACGAATTCTG TTGATGTGAC AGTTGGTCCA 
AGAGCATGCT TGAACTGTCC TCTAACTGTC GATGGATCCC GTGAATGGTT CATTCGATTG 
TGGAATGAGA ACTTCATTCC ATATTTGGAA CGTGTTGCTA GAGATGGCAA AAAAACCTTC 
GGTCGCTGCA CTTCCTTCGA GGATCCCACC GACATCGTCT CTGAAAAATG GCCGTGGTTC 
GATGGTGAAA ACCCGGAGAA TGTGCTCAAA CGTCTTCAAC TCCAAGACCT CGTCCCGTCA 
CCTGCCAACT CATCCCGACA ACACTTCAAT CCCCTCGAGT CGTTGATCCA ATTGCATGCT 
ACCAAGCATC AGACCATCGA CAACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022169.1
CDS	42..4691
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWALSVDSQIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001026998.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026998.4

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGGCGCTTT CCGTAGATTC TCAGATCCGC TCCTCACTCT CCAAGTTCAC CAAGAAGAAG 
AACAAGAACT ACGACGAAGC ACATATGCCA TCAATTTCCG GATCTCAAGG AACTCTTGAC 
AACATTGATG TGATTGAGTT GAAGCAAGAG CTCAAAGAAC GCGATAGTGC ACTTTACGAA 
GTCCGCCTTG ACAATCTGGA TCGTGCCCGC GAAGTTGATG TTCTGAGGGA GACAGTGAAC 
AAGTTGAAAA CCGAGAACAA GCAATTAAAG AAAGAAGTGG ACAAACTCAC CAACGGTCCA 
GCCACTCGTG CTTCTTCCCG CGCCTCAATT CCAGTTATCT ACGACGATGA GCATGTCTAT 
GATGCAGCGT GTAGCAGTAC ATCAGCTAGT CAATCTTCGA AACGATCCTC TGGCTGCAAC 
TCAATCAAGG TTACTGTAAA CGTGGACATC GCTGGAGAAA TCAGTTCGAT CGTTAACCCG 
GACAAAGAGA TAATCGTAGG ATATCTTGCC ATGCCAACCA GTCAGTCATG CTGGAAAGAC 
ATTGATGTTT CTATTCTAGG ACTATTTGAA GTCTACCTAT CCAGAATTGA TGTGGAGCAT 
CAACTTGGAA TCGATGCTCG TGATTCTATC CTTGGCTATC AAATTGGTGA ACTTCGACGC 
GTCATTGGAG ACTCCACAAC CATGATAACC AGCCATCCAA CTGACATTCT TACTTCCTCA 
ACTACAATCC GAATGTTCAT GCACGGTGCC GCACAGAGTC GCGTAGACAG TCTGGTCCTT 
GATATGCTTC TTCCAAAGCA AATGATTCTC CAACTCGTCA AGTCAATTTT GACAGAGAGA 
CGTCTGGTGT TAGCTGGAGC AACTGGAATT GGAAAGAGCA AACTGGCGAA GACCCTGGCT 
GCTTATGTAT CTATTCGAAC AAATCAATCC GAAGATAGTA TTGTTAATAT CAGCATTCCT 
GAAAACAATA AAGAAGAATT GCTTCAAGTG GAACGACGCC TGGAAAAGAT CTTGAGAAGC 
AAAGAATCAT GCATCGTAAT TCTAGATAAT ATCCCAAAGA ATCGAATTGC ATTTGTTGTA 
TCCGTTTTTG CAAATGTCCC ACTTCAAAAC AACGAAGGTC CATTTGTAGT ATGCACAGTC 
AACCGATATC AAATCCCTGA GCTTCAAATT CACCACAATT TCAAAATGTC AGTAATGTCG 
AATCGTCTCG AAGGATTCAT CCTACGTTAC CTCCGACGAC GGGCGGTAGA GGATGAGTAT 
CGTCTAACTG TACAGATGCC ATCAGAGCTC TTCAAAATCA TTGACTTCTT CCCAATAGCT 
CTTCAGGCCG TCAATAATTT TATTGAGAAA ACGAATTCTG TTGATGTGAC AGTTGGTCCA 
AGAGCATGCT TGAACTGTCC TCTAACTGTC GATGGATCCC GTGAATGGTT CATTCGATTG 
TGGAATGAGA ACTTCATTCC ATATTTGGAA CGTGTTGCTA GAGATGGCAA AAAAACCTTC 
GGTCGCTGCA CTTCCTTCGA GGATCCCACC GACATCGTCT CTGAAAAATG GCCGTGGTTC 
GATGGTGAAA ACCCGGAGAA TGTGCTCAAA CGTCTTCAAC TCCAAGACCT CGTCCCGTCA 
CCTGCCAACT CATCCCGACA ACACTTCAAT CCCCTCGAGT CGTTGATCCA ATTGCATGCT 
ACCAAGCATC AGACCATCGA CAACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001026998.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022169.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Price & Availability↑

CloneID	OCa338200
Clone ID Related Accession (Same CDS sequence)	NM_001026998.4 , NM_001026998.3
Accession Version	NM_001026998.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001026998.2. COMPLETENESS: incomplete on both ends.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGGCGCTTT CCGTAGATTC TCAGATCCGC TCCTCACTCT CCAAGTTCAC CAAGAAGAAG 
AACAAGAACT ACGACGAAGC ACATATGCCA TCAATTTCCG GATCTCAAGG AACTCTTGAC 
AACATTGATG TGATTGAGTT GAAGCAAGAG CTCAAAGAAC GCGATAGTGC ACTTTACGAA 
GTCCGCCTTG ACAATCTGGA TCGTGCCCGC GAAGTTGATG TTCTGAGGGA GACAGTGAAC 
AAGTTGAAAA CCGAGAACAA GCAATTAAAG AAAGAAGTGG ACAAACTCAC CAACGGTCCA 
GCCACTCGTG CTTCTTCCCG CGCCTCAATT CCAGTTATCT ACGACGATGA GCATGTCTAT 
GATGCAGCGT GTAGCAGTAC ATCAGCTAGT CAATCTTCGA AACGATCCTC TGGCTGCAAC 
TCAATCAAGG TTACTGTAAA CGTGGACATC GCTGGAGAAA TCAGTTCGAT CGTTAACCCG 
GACAAAGAGA TAATCGTAGG ATATCTTGCC ATGCCAACCA GTCAGTCATG CTGGAAAGAC 
ATTGATGTTT CTATTCTAGG ACTATTTGAA GTCTACCTAT CCAGAATTGA TGTGGAGCAT 
CAACTTGGAA TCGATGCTCG TGATTCTATC CTTGGCTATC AAATTGGTGA ACTTCGACGC 
GTCATTGGAG ACTCCACAAC CATGATAACC AGCCATCCAA CTGACATTCT TACTTCCTCA 
ACTACAATCC GAATGTTCAT GCACGGTGCC GCACAGAGTC GCGTAGACAG TCTGGTCCTT 
GATATGCTTC TTCCAAAGCA AATGATTCTC CAACTCGTCA AGTCAATTTT GACAGAGAGA 
CGTCTGGTGT TAGCTGGAGC AACTGGAATT GGAAAGAGCA AACTGGCGAA GACCCTGGCT 
GCTTATGTAT CTATTCGAAC AAATCAATCC GAAGATAGTA TTGTTAATAT CAGCATTCCT 
GAAAACAATA AAGAAGAATT GCTTCAAGTG GAACGACGCC TGGAAAAGAT CTTGAGAAGC 
AAAGAATCAT GCATCGTAAT TCTAGATAAT ATCCCAAAGA ATCGAATTGC ATTTGTTGTA 
TCCGTTTTTG CAAATGTCCC ACTTCAAAAC AACGAAGGTC CATTTGTAGT ATGCACAGTC 
AACCGATATC AAATCCCTGA GCTTCAAATT CACCACAATT TCAAAATGTC AGTAATGTCG 
AATCGTCTCG AAGGATTCAT CCTACGTTAC CTCCGACGAC GGGCGGTAGA GGATGAGTAT 
CGTCTAACTG TACAGATGCC ATCAGAGCTC TTCAAAATCA TTGACTTCTT CCCAATAGCT 
CTTCAGGCCG TCAATAATTT TATTGAGAAA ACGAATTCTG TTGATGTGAC AGTTGGTCCA 
AGAGCATGCT TGAACTGTCC TCTAACTGTC GATGGATCCC GTGAATGGTT CATTCGATTG 
TGGAATGAGA ACTTCATTCC ATATTTGGAA CGTGTTGCTA GAGATGGCAA AAAAACCTTC 
GGTCGCTGCA CTTCCTTCGA GGATCCCACC GACATCGTCT CTGAAAAATG GCCGTGGTTC 
GATGGTGAAA ACCCGGAGAA TGTGCTCAAA CGTCTTCAAC TCCAAGACCT CGTCCCGTCA 
CCTGCCAACT CATCCCGACA ACACTTCAAT CCCCTCGAGT CGTTGATCCA ATTGCATGCT 
ACCAAGCATC AGACCATCGA CAACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022169.1
CDS	1..4650
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWALSVDSQIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001026998.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026998.3

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGGCGCTTT CCGTAGATTC TCAGATCCGC TCCTCACTCT CCAAGTTCAC CAAGAAGAAG 
AACAAGAACT ACGACGAAGC ACATATGCCA TCAATTTCCG GATCTCAAGG AACTCTTGAC 
AACATTGATG TGATTGAGTT GAAGCAAGAG CTCAAAGAAC GCGATAGTGC ACTTTACGAA 
GTCCGCCTTG ACAATCTGGA TCGTGCCCGC GAAGTTGATG TTCTGAGGGA GACAGTGAAC 
AAGTTGAAAA CCGAGAACAA GCAATTAAAG AAAGAAGTGG ACAAACTCAC CAACGGTCCA 
GCCACTCGTG CTTCTTCCCG CGCCTCAATT CCAGTTATCT ACGACGATGA GCATGTCTAT 
GATGCAGCGT GTAGCAGTAC ATCAGCTAGT CAATCTTCGA AACGATCCTC TGGCTGCAAC 
TCAATCAAGG TTACTGTAAA CGTGGACATC GCTGGAGAAA TCAGTTCGAT CGTTAACCCG 
GACAAAGAGA TAATCGTAGG ATATCTTGCC ATGCCAACCA GTCAGTCATG CTGGAAAGAC 
ATTGATGTTT CTATTCTAGG ACTATTTGAA GTCTACCTAT CCAGAATTGA TGTGGAGCAT 
CAACTTGGAA TCGATGCTCG TGATTCTATC CTTGGCTATC AAATTGGTGA ACTTCGACGC 
GTCATTGGAG ACTCCACAAC CATGATAACC AGCCATCCAA CTGACATTCT TACTTCCTCA 
ACTACAATCC GAATGTTCAT GCACGGTGCC GCACAGAGTC GCGTAGACAG TCTGGTCCTT 
GATATGCTTC TTCCAAAGCA AATGATTCTC CAACTCGTCA AGTCAATTTT GACAGAGAGA 
CGTCTGGTGT TAGCTGGAGC AACTGGAATT GGAAAGAGCA AACTGGCGAA GACCCTGGCT 
GCTTATGTAT CTATTCGAAC AAATCAATCC GAAGATAGTA TTGTTAATAT CAGCATTCCT 
GAAAACAATA AAGAAGAATT GCTTCAAGTG GAACGACGCC TGGAAAAGAT CTTGAGAAGC 
AAAGAATCAT GCATCGTAAT TCTAGATAAT ATCCCAAAGA ATCGAATTGC ATTTGTTGTA 
TCCGTTTTTG CAAATGTCCC ACTTCAAAAC AACGAAGGTC CATTTGTAGT ATGCACAGTC 
AACCGATATC AAATCCCTGA GCTTCAAATT CACCACAATT TCAAAATGTC AGTAATGTCG 
AATCGTCTCG AAGGATTCAT CCTACGTTAC CTCCGACGAC GGGCGGTAGA GGATGAGTAT 
CGTCTAACTG TACAGATGCC ATCAGAGCTC TTCAAAATCA TTGACTTCTT CCCAATAGCT 
CTTCAGGCCG TCAATAATTT TATTGAGAAA ACGAATTCTG TTGATGTGAC AGTTGGTCCA 
AGAGCATGCT TGAACTGTCC TCTAACTGTC GATGGATCCC GTGAATGGTT CATTCGATTG 
TGGAATGAGA ACTTCATTCC ATATTTGGAA CGTGTTGCTA GAGATGGCAA AAAAACCTTC 
GGTCGCTGCA CTTCCTTCGA GGATCCCACC GACATCGTCT CTGAAAAATG GCCGTGGTTC 
GATGGTGAAA ACCCGGAGAA TGTGCTCAAA CGTCTTCAAC TCCAAGACCT CGTCCCGTCA 
CCTGCCAACT CATCCCGACA ACACTTCAAT CCCCTCGAGT CGTTGATCCA ATTGCATGCT 
ACCAAGCATC AGACCATCGA CAACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001027000.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022171.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa339828
Clone ID Related Accession (Same CDS sequence)	NM_001027000.4 , NM_001027000.3
Accession Version	NM_001027000.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001027000.3. COMPLETENESS: incomplete on the 5' end.

ATGCCACCCG CGGTTTCTAA ATTACCCTCG CCACGTGTCG CCACGTCAGC AACCGCTTCA 
GCAACTAACC CAAATTCCAA CTTTCCACAA ATGTCAACAT CCAGGCTTCA GACTCCACAG 
TCAAGAATAT CGAAAATTGA TTCATCAAAG ATTGGTATCA AGCCAAAGAC GTCTGGACTT 
AAACCACCCT CATCATCAAC CACTTCATCA AATAATACAA ATTCATTCCG TCCGTCGAGC 
CGTTCGAGTG GCAATAATAA TGTTGGCTCG ACGATATCCA CATCTGCGAA GAGCTTAGAA 
TCATCATCAA CGTACAGCTC TATTTCGAAT CTAAACCGAC CTACCTCCCA ACTCCAAAAA 
CCTTCTAGAC CACAAACCCA GCTAGTTCGT GTTGCTACAA CTACAAAAAT CGGAAGCTCA 
AAGCTAGCCG CTCCGAAAGC CGTGAGCACC CCAAAACTTG CTTCTGTGAA GACTATTGGA 
GCAAAACAAG AGCCCGATAA CAGCGGTGGT GGTGGTGGTG GAATGCTGAA ATTAAAGTTA 
TTCAGTAGCA AAAACCCATC TTCCTCATCG AATAGCCCAC AACCTACGAG AAAGGCGGCG 
GCGGTGCCTC AACAACAAAC TTTGTCGAAA ATCGCTGCCC CAGTGAAAAG TGGCCTGAAG 
CCGCCGACCA GTAAGCTGGG AAGTGCCACG TCTATGTCGA AGCTTTGTAC GCCAAAAGTT 
TCCTACCGTA AAACGGACGC CCCAATCATA TCTCAACAAG ACTCGAAACG ATGCTCAAAG 
AGCAGTGAAG AAGAGTCCGG ATACGCTGGA TTCAACAGCA CGTCGCCAAC GTCATCATCG 
ACGGAAGGTT CCCTAAGCAT GCATTCCACA TCTTCCAAGA GTTCAACGTC AGACGAAAAG 
TCTCCGTCAT CAGACGATCT TACTCTTAAC GCCTCCATCG TGACAGCTAT CAGACAGCCG 
ATAGCCGCAA CACCGGTTTC TCCAAATATT ATCAACAAGC CTGTTGAGGA AAAACCAACA 
CTGGCAGTGA AAGGAGTGAA AAGCACAGCG AAAAAAGATC CACCTCCAGC TGTTCCGCCA 
CGTGACACCC AGCCAACAAT CGGAGTTGTT AGTCCAATTA TGGCACATAA GAAGTTGACA 
AATGACCCCG TGATATCTGA AAAACCAGAA CCTGAAAAGC TCCAATCAAT GAGCATCGAC 
ACGACGGACG TTCCACCGCT TCCACCTCTA AAATCAGTTG TTCCACTTAA AATGACTTCA 
ATCCGACAAC CACCAACGTA CGATGTTCTT CTAAAACAAG GAAAAATCAC ATCGCCTGTC 
AAGTCGTTTG GATATGAGCA GTCGTCCGCG TCTGAAGACT CCATTGTGGC TCATGCGTCG 
GCTCAGGTGA CTCCGCCGAC AAAAACTTCT GGTAATCATT CGCTGGAGAG AAGGATGGGA 
AAGAATAAGA CATCAGAATC CAGCGGCTAC ACCTCTGACG CCGGTGTTGC GATGTGCGCC 
AAAATGAGGG AGAAGCTGAA AGAATACGAT GACATGACTC GTCGAGCACA GAACGGCTAT 
CCTGACAACT TCGAAGACAG TTCCTCCTTG TCGTCTGGAA TATCCGATAA CAACGAGCTC 
GACGACATAT CCACGGACGA TTTGTCCGGA GTAGACATGG CAACAGTCGC CTCCAAACAT 
AGCGACTATT CCCACTTTGT TCGCCATCCC ACGTCTTCTT CCTCAAAGCC CCGAGTCCCC 
AGTCGGTCCT CCACATCAGT CGATTCTCGA TCTCGAGCAG AACAGGAGAA TGTGTACAAA 
CTTCTGTCCC AGTGCCGAAC GAGCCAACGT GGCGCCGCTG CCACCTCAAC CTTCGGACAA 
CATTCGCTAA GATCCCCGGG ATACTCATCC TATTCTCCAC ACTTATCAGT GTCAGCTGAT 
AAGGACACAA TGTCTATGCA CTCACAGACT AGTCGACGAC CTTCTTCACA AAAACCAAGC 
TATTCAGGCC AATTTCATTC ACTTGATCGT AAATGCCACC TTCAAGAGTT CACATCCACC 
GAGCACAGAA TGGCGGCTCT CTTGAGCCCG AGACGGGTGC CGAACTCGAT GTCGAAATAT 
GATTCTTCAG CAGCCGCCCT CAACGCTTCG GGTATGTCTC GTTCAATGAT CCTTCTCGAA 
TCCCTTTCAC CACGACCACC TCGACGTCAC CAATCACCGG CTGACTCGTG CATTATCACA 
GCTTCTCCAT CAGCCCCACG AAGATCACAT TCGCCACGTG GCCCCACCGC CCGTATCCCA 
CTTTCTCTCG CTTCTTCGCC TGTCCACGTC AATAACAATT GGGGATCCTA CTCGGCGCGT 
TCCCGAGGTG GAAGCTCTAC TGGTATCTAT GGAGAGACGT TCCAACTGCA CAGACTATCC 
GATGAAAAAT CCCCCGCACA TTCTGCCAAA AGTGAGATGG GATCCCAACT ATCACTGGCT 
AGCACGACAG CATATGGATC TCTCAATGAG AAGTACGAAC ATGCTATTCG GGACATGGCA 
CGTGACTTGG AGTGTTACAA GAACACTGTC GACTCACTAA CCAAGAAACA GGAGAACTAT 
GGAGCATTGT TTGATCTTTT TGAGCAAAAG CTTAGAAAAC TCACTCAACA CATTGATCGA 
TCCAACTTGA AGCCTGAAGA GGCAATACGA TTCAGGCAGG ACATTGCTCA TTTGAGGGAT 
ATTAGCAATC ATCTTGCATC CAACTCAGCT CATGCTAACG AAGGCGCTGG TGAGCTTCTT 
CGTCAACCAT CTCTGGAATC AGTTGCATCC CATCGATCAT CGATGTCATC GTCGTCGAAA 
AGCAGCAAGC AGGAGAAGAT CAGCTTGAGC TCGTTTGGCA AGAACAAGAA GAGCTGGATC 
CGCTCCTCAC TCTCCAAGTT CACCAAGAAG AAGAACAAGA ACTACGACGA AGCACATATG 
CCATCAATTT CCGGATCTCA AGGAACTCTT GACAACATTG ATGTGATTGA GTTGAAGCAA 
GAGCTCAAAG AACGCGATAG TGCACTTTAC GAAGTCCGCC TTGACAATCT GGATCGTGCC 
CGCGAAGTTG ATGTTCTGAG GGAGACAGTG AACAAGTTGA AAACCGAGAA CAAGCAATTA 
AAGAAAGAAG TGGACAAACT CACCAACGGT CCAGCCACTC GTGCTTCTTC CCGCGCCTCA 
ATTCCAGTTA TCTACGACGA TGAGCATGTC TATGATGCAG CGTGTAGCAG TACATCAGCT 
AGTCAATCTT CGAAACGATC CTCTGGCTGC AACTCAATCA AGGTTACTGT AAACGTGGAC 
ATCGCTGGAG AAATCAGTTC GATCGTTAAC CCGGACAAAG AGATAATCGT AGGATATCTT 
GCCATGCCAA CCAGTCAGTC ATGCTGGAAA GACATTGATG TTTCTATTCT AGGACTATTT 
GAAGTCTACC TATCCAGAAT TGATGTGGAG CATCAACTTG GAATCGATGC TCGTGATTCT 
ATCCTTGGCT ATCAAATTGG TGAACTTCGA CGCGTCATTG GAGACTCCAC AACCATGATA 
ACCAGCCATC CAACTGACAT TCTTACTTCC TCAACTACAA TCCGAATGTT CATGCACGGT 
GCCGCACAGA GTCGCGTAGA CAGTCTGGTC CTTGATATGC TTCTTCCAAA GCAAATGATT 
CTCCAACTCG TCAAGTCAAT TTTGACAGAG AGACGTCTGG TGTTAGCTGG AGCAACTGGA 
ATTGGAAAGA GCAAACTGGC GAAGACCCTG GCTGCTTATG TATCTATTCG AACAAATCAA 
TCCGAAGATA GTATTGTTAA TATCAGCATT CCTGAAAACA ATAAAGAAGA ATTGCTTCAA 
GTGGAACGAC GCCTGGAAAA GATCTTGAGA AGCAAAGAAT CATGCATCGT AATTCTAGAT 
AATATCCCAA AGAATCGAAT TGCATTTGTT GTATCCGTTT TTGCAAATGT CCCACTTCAA 
AACAACGAAG GTCCATTTGT AGTATGCACA GTCAACCGAT ATCAAATCCC TGAGCTTCAA 
ATTCACCACA ATTTCAAAAT GTCAGTAATG TCGAATCGTC TCGAAGGATT CATCCTACGT 
TACCTCCGAC GACGGGCGGT AGAGGATGAG TATCGTCTAA CTGTACAGAT GCCATCAGAG 
CTCTTCAAAA TCATTGACTT CTTCCCAATA GCTCTTCAGG CCGTCAATAA TTTTATTGAG 
AAAACGAATT CTGTTGATGT GACAGTTGGT CCAAGAGCAT GCTTGAACTG TCCTCTAACT 
GTCGATGGAT CCCGTGAATG GTTCATTCGA TTGTGGAATG AGAACTTCAT TCCATATTTG 
GAACGTGTTG CTAGAGATGG CAAAAAAACC TTCGGTCGCT GCACTTCCTT CGAGGATCCC 
ACCGACATCG TCTCTGAAAA ATGGCCGTGG TTCGATGGTG AAAACCCGGA GAATGTGCTC 
AAACGTCTTC AACTCCAAGA CCTCGTCCCG TCACCTGCCA ACTCATCCCG ACAACACTTC 
AATCCCCTCG AGTCGTTGAT CCAATTGCAT GCTACCAAGC ATCAGACCAT CGACAACATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022171.1
CDS	1..4563
Translation	MPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001027000.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027000.4

ORF Insert Sequence:

ATGCCACCCG CGGTTTCTAA ATTACCCTCG CCACGTGTCG CCACGTCAGC AACCGCTTCA 
GCAACTAACC CAAATTCCAA CTTTCCACAA ATGTCAACAT CCAGGCTTCA GACTCCACAG 
TCAAGAATAT CGAAAATTGA TTCATCAAAG ATTGGTATCA AGCCAAAGAC GTCTGGACTT 
AAACCACCCT CATCATCAAC CACTTCATCA AATAATACAA ATTCATTCCG TCCGTCGAGC 
CGTTCGAGTG GCAATAATAA TGTTGGCTCG ACGATATCCA CATCTGCGAA GAGCTTAGAA 
TCATCATCAA CGTACAGCTC TATTTCGAAT CTAAACCGAC CTACCTCCCA ACTCCAAAAA 
CCTTCTAGAC CACAAACCCA GCTAGTTCGT GTTGCTACAA CTACAAAAAT CGGAAGCTCA 
AAGCTAGCCG CTCCGAAAGC CGTGAGCACC CCAAAACTTG CTTCTGTGAA GACTATTGGA 
GCAAAACAAG AGCCCGATAA CAGCGGTGGT GGTGGTGGTG GAATGCTGAA ATTAAAGTTA 
TTCAGTAGCA AAAACCCATC TTCCTCATCG AATAGCCCAC AACCTACGAG AAAGGCGGCG 
GCGGTGCCTC AACAACAAAC TTTGTCGAAA ATCGCTGCCC CAGTGAAAAG TGGCCTGAAG 
CCGCCGACCA GTAAGCTGGG AAGTGCCACG TCTATGTCGA AGCTTTGTAC GCCAAAAGTT 
TCCTACCGTA AAACGGACGC CCCAATCATA TCTCAACAAG ACTCGAAACG ATGCTCAAAG 
AGCAGTGAAG AAGAGTCCGG ATACGCTGGA TTCAACAGCA CGTCGCCAAC GTCATCATCG 
ACGGAAGGTT CCCTAAGCAT GCATTCCACA TCTTCCAAGA GTTCAACGTC AGACGAAAAG 
TCTCCGTCAT CAGACGATCT TACTCTTAAC GCCTCCATCG TGACAGCTAT CAGACAGCCG 
ATAGCCGCAA CACCGGTTTC TCCAAATATT ATCAACAAGC CTGTTGAGGA AAAACCAACA 
CTGGCAGTGA AAGGAGTGAA AAGCACAGCG AAAAAAGATC CACCTCCAGC TGTTCCGCCA 
CGTGACACCC AGCCAACAAT CGGAGTTGTT AGTCCAATTA TGGCACATAA GAAGTTGACA 
AATGACCCCG TGATATCTGA AAAACCAGAA CCTGAAAAGC TCCAATCAAT GAGCATCGAC 
ACGACGGACG TTCCACCGCT TCCACCTCTA AAATCAGTTG TTCCACTTAA AATGACTTCA 
ATCCGACAAC CACCAACGTA CGATGTTCTT CTAAAACAAG GAAAAATCAC ATCGCCTGTC 
AAGTCGTTTG GATATGAGCA GTCGTCCGCG TCTGAAGACT CCATTGTGGC TCATGCGTCG 
GCTCAGGTGA CTCCGCCGAC AAAAACTTCT GGTAATCATT CGCTGGAGAG AAGGATGGGA 
AAGAATAAGA CATCAGAATC CAGCGGCTAC ACCTCTGACG CCGGTGTTGC GATGTGCGCC 
AAAATGAGGG AGAAGCTGAA AGAATACGAT GACATGACTC GTCGAGCACA GAACGGCTAT 
CCTGACAACT TCGAAGACAG TTCCTCCTTG TCGTCTGGAA TATCCGATAA CAACGAGCTC 
GACGACATAT CCACGGACGA TTTGTCCGGA GTAGACATGG CAACAGTCGC CTCCAAACAT 
AGCGACTATT CCCACTTTGT TCGCCATCCC ACGTCTTCTT CCTCAAAGCC CCGAGTCCCC 
AGTCGGTCCT CCACATCAGT CGATTCTCGA TCTCGAGCAG AACAGGAGAA TGTGTACAAA 
CTTCTGTCCC AGTGCCGAAC GAGCCAACGT GGCGCCGCTG CCACCTCAAC CTTCGGACAA 
CATTCGCTAA GATCCCCGGG ATACTCATCC TATTCTCCAC ACTTATCAGT GTCAGCTGAT 
AAGGACACAA TGTCTATGCA CTCACAGACT AGTCGACGAC CTTCTTCACA AAAACCAAGC 
TATTCAGGCC AATTTCATTC ACTTGATCGT AAATGCCACC TTCAAGAGTT CACATCCACC 
GAGCACAGAA TGGCGGCTCT CTTGAGCCCG AGACGGGTGC CGAACTCGAT GTCGAAATAT 
GATTCTTCAG CAGCCGCCCT CAACGCTTCG GGTATGTCTC GTTCAATGAT CCTTCTCGAA 
TCCCTTTCAC CACGACCACC TCGACGTCAC CAATCACCGG CTGACTCGTG CATTATCACA 
GCTTCTCCAT CAGCCCCACG AAGATCACAT TCGCCACGTG GCCCCACCGC CCGTATCCCA 
CTTTCTCTCG CTTCTTCGCC TGTCCACGTC AATAACAATT GGGGATCCTA CTCGGCGCGT 
TCCCGAGGTG GAAGCTCTAC TGGTATCTAT GGAGAGACGT TCCAACTGCA CAGACTATCC 
GATGAAAAAT CCCCCGCACA TTCTGCCAAA AGTGAGATGG GATCCCAACT ATCACTGGCT 
AGCACGACAG CATATGGATC TCTCAATGAG AAGTACGAAC ATGCTATTCG GGACATGGCA 
CGTGACTTGG AGTGTTACAA GAACACTGTC GACTCACTAA CCAAGAAACA GGAGAACTAT 
GGAGCATTGT TTGATCTTTT TGAGCAAAAG CTTAGAAAAC TCACTCAACA CATTGATCGA 
TCCAACTTGA AGCCTGAAGA GGCAATACGA TTCAGGCAGG ACATTGCTCA TTTGAGGGAT 
ATTAGCAATC ATCTTGCATC CAACTCAGCT CATGCTAACG AAGGCGCTGG TGAGCTTCTT 
CGTCAACCAT CTCTGGAATC AGTTGCATCC CATCGATCAT CGATGTCATC GTCGTCGAAA 
AGCAGCAAGC AGGAGAAGAT CAGCTTGAGC TCGTTTGGCA AGAACAAGAA GAGCTGGATC 
CGCTCCTCAC TCTCCAAGTT CACCAAGAAG AAGAACAAGA ACTACGACGA AGCACATATG 
CCATCAATTT CCGGATCTCA AGGAACTCTT GACAACATTG ATGTGATTGA GTTGAAGCAA 
GAGCTCAAAG AACGCGATAG TGCACTTTAC GAAGTCCGCC TTGACAATCT GGATCGTGCC 
CGCGAAGTTG ATGTTCTGAG GGAGACAGTG AACAAGTTGA AAACCGAGAA CAAGCAATTA 
AAGAAAGAAG TGGACAAACT CACCAACGGT CCAGCCACTC GTGCTTCTTC CCGCGCCTCA 
ATTCCAGTTA TCTACGACGA TGAGCATGTC TATGATGCAG CGTGTAGCAG TACATCAGCT 
AGTCAATCTT CGAAACGATC CTCTGGCTGC AACTCAATCA AGGTTACTGT AAACGTGGAC 
ATCGCTGGAG AAATCAGTTC GATCGTTAAC CCGGACAAAG AGATAATCGT AGGATATCTT 
GCCATGCCAA CCAGTCAGTC ATGCTGGAAA GACATTGATG TTTCTATTCT AGGACTATTT 
GAAGTCTACC TATCCAGAAT TGATGTGGAG CATCAACTTG GAATCGATGC TCGTGATTCT 
ATCCTTGGCT ATCAAATTGG TGAACTTCGA CGCGTCATTG GAGACTCCAC AACCATGATA 
ACCAGCCATC CAACTGACAT TCTTACTTCC TCAACTACAA TCCGAATGTT CATGCACGGT 
GCCGCACAGA GTCGCGTAGA CAGTCTGGTC CTTGATATGC TTCTTCCAAA GCAAATGATT 
CTCCAACTCG TCAAGTCAAT TTTGACAGAG AGACGTCTGG TGTTAGCTGG AGCAACTGGA 
ATTGGAAAGA GCAAACTGGC GAAGACCCTG GCTGCTTATG TATCTATTCG AACAAATCAA 
TCCGAAGATA GTATTGTTAA TATCAGCATT CCTGAAAACA ATAAAGAAGA ATTGCTTCAA 
GTGGAACGAC GCCTGGAAAA GATCTTGAGA AGCAAAGAAT CATGCATCGT AATTCTAGAT 
AATATCCCAA AGAATCGAAT TGCATTTGTT GTATCCGTTT TTGCAAATGT CCCACTTCAA 
AACAACGAAG GTCCATTTGT AGTATGCACA GTCAACCGAT ATCAAATCCC TGAGCTTCAA 
ATTCACCACA ATTTCAAAAT GTCAGTAATG TCGAATCGTC TCGAAGGATT CATCCTACGT 
TACCTCCGAC GACGGGCGGT AGAGGATGAG TATCGTCTAA CTGTACAGAT GCCATCAGAG 
CTCTTCAAAA TCATTGACTT CTTCCCAATA GCTCTTCAGG CCGTCAATAA TTTTATTGAG 
AAAACGAATT CTGTTGATGT GACAGTTGGT CCAAGAGCAT GCTTGAACTG TCCTCTAACT 
GTCGATGGAT CCCGTGAATG GTTCATTCGA TTGTGGAATG AGAACTTCAT TCCATATTTG 
GAACGTGTTG CTAGAGATGG CAAAAAAACC TTCGGTCGCT GCACTTCCTT CGAGGATCCC 
ACCGACATCG TCTCTGAAAA ATGGCCGTGG TTCGATGGTG AAAACCCGGA GAATGTGCTC 
AAACGTCTTC AACTCCAAGA CCTCGTCCCG TCACCTGCCA ACTCATCCCG ACAACACTTC 
AATCCCCTCG AGTCGTTGAT CCAATTGCAT GCTACCAAGC ATCAGACCAT CGACAACATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001027000.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001022171.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa339828
Clone ID Related Accession (Same CDS sequence)	NM_001027000.4 , NM_001027000.3
Accession Version	NM_001027000.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_001027000.2. COMPLETENESS: incomplete on both ends.

ATGCCACCCG CGGTTTCTAA ATTACCCTCG CCACGTGTCG CCACGTCAGC AACCGCTTCA 
GCAACTAACC CAAATTCCAA CTTTCCACAA ATGTCAACAT CCAGGCTTCA GACTCCACAG 
TCAAGAATAT CGAAAATTGA TTCATCAAAG ATTGGTATCA AGCCAAAGAC GTCTGGACTT 
AAACCACCCT CATCATCAAC CACTTCATCA AATAATACAA ATTCATTCCG TCCGTCGAGC 
CGTTCGAGTG GCAATAATAA TGTTGGCTCG ACGATATCCA CATCTGCGAA GAGCTTAGAA 
TCATCATCAA CGTACAGCTC TATTTCGAAT CTAAACCGAC CTACCTCCCA ACTCCAAAAA 
CCTTCTAGAC CACAAACCCA GCTAGTTCGT GTTGCTACAA CTACAAAAAT CGGAAGCTCA 
AAGCTAGCCG CTCCGAAAGC CGTGAGCACC CCAAAACTTG CTTCTGTGAA GACTATTGGA 
GCAAAACAAG AGCCCGATAA CAGCGGTGGT GGTGGTGGTG GAATGCTGAA ATTAAAGTTA 
TTCAGTAGCA AAAACCCATC TTCCTCATCG AATAGCCCAC AACCTACGAG AAAGGCGGCG 
GCGGTGCCTC AACAACAAAC TTTGTCGAAA ATCGCTGCCC CAGTGAAAAG TGGCCTGAAG 
CCGCCGACCA GTAAGCTGGG AAGTGCCACG TCTATGTCGA AGCTTTGTAC GCCAAAAGTT 
TCCTACCGTA AAACGGACGC CCCAATCATA TCTCAACAAG ACTCGAAACG ATGCTCAAAG 
AGCAGTGAAG AAGAGTCCGG ATACGCTGGA TTCAACAGCA CGTCGCCAAC GTCATCATCG 
ACGGAAGGTT CCCTAAGCAT GCATTCCACA TCTTCCAAGA GTTCAACGTC AGACGAAAAG 
TCTCCGTCAT CAGACGATCT TACTCTTAAC GCCTCCATCG TGACAGCTAT CAGACAGCCG 
ATAGCCGCAA CACCGGTTTC TCCAAATATT ATCAACAAGC CTGTTGAGGA AAAACCAACA 
CTGGCAGTGA AAGGAGTGAA AAGCACAGCG AAAAAAGATC CACCTCCAGC TGTTCCGCCA 
CGTGACACCC AGCCAACAAT CGGAGTTGTT AGTCCAATTA TGGCACATAA GAAGTTGACA 
AATGACCCCG TGATATCTGA AAAACCAGAA CCTGAAAAGC TCCAATCAAT GAGCATCGAC 
ACGACGGACG TTCCACCGCT TCCACCTCTA AAATCAGTTG TTCCACTTAA AATGACTTCA 
ATCCGACAAC CACCAACGTA CGATGTTCTT CTAAAACAAG GAAAAATCAC ATCGCCTGTC 
AAGTCGTTTG GATATGAGCA GTCGTCCGCG TCTGAAGACT CCATTGTGGC TCATGCGTCG 
GCTCAGGTGA CTCCGCCGAC AAAAACTTCT GGTAATCATT CGCTGGAGAG AAGGATGGGA 
AAGAATAAGA CATCAGAATC CAGCGGCTAC ACCTCTGACG CCGGTGTTGC GATGTGCGCC 
AAAATGAGGG AGAAGCTGAA AGAATACGAT GACATGACTC GTCGAGCACA GAACGGCTAT 
CCTGACAACT TCGAAGACAG TTCCTCCTTG TCGTCTGGAA TATCCGATAA CAACGAGCTC 
GACGACATAT CCACGGACGA TTTGTCCGGA GTAGACATGG CAACAGTCGC CTCCAAACAT 
AGCGACTATT CCCACTTTGT TCGCCATCCC ACGTCTTCTT CCTCAAAGCC CCGAGTCCCC 
AGTCGGTCCT CCACATCAGT CGATTCTCGA TCTCGAGCAG AACAGGAGAA TGTGTACAAA 
CTTCTGTCCC AGTGCCGAAC GAGCCAACGT GGCGCCGCTG CCACCTCAAC CTTCGGACAA 
CATTCGCTAA GATCCCCGGG ATACTCATCC TATTCTCCAC ACTTATCAGT GTCAGCTGAT 
AAGGACACAA TGTCTATGCA CTCACAGACT AGTCGACGAC CTTCTTCACA AAAACCAAGC 
TATTCAGGCC AATTTCATTC ACTTGATCGT AAATGCCACC TTCAAGAGTT CACATCCACC 
GAGCACAGAA TGGCGGCTCT CTTGAGCCCG AGACGGGTGC CGAACTCGAT GTCGAAATAT 
GATTCTTCAG CAGCCGCCCT CAACGCTTCG GGTATGTCTC GTTCAATGAT CCTTCTCGAA 
TCCCTTTCAC CACGACCACC TCGACGTCAC CAATCACCGG CTGACTCGTG CATTATCACA 
GCTTCTCCAT CAGCCCCACG AAGATCACAT TCGCCACGTG GCCCCACCGC CCGTATCCCA 
CTTTCTCTCG CTTCTTCGCC TGTCCACGTC AATAACAATT GGGGATCCTA CTCGGCGCGT 
TCCCGAGGTG GAAGCTCTAC TGGTATCTAT GGAGAGACGT TCCAACTGCA CAGACTATCC 
GATGAAAAAT CCCCCGCACA TTCTGCCAAA AGTGAGATGG GATCCCAACT ATCACTGGCT 
AGCACGACAG CATATGGATC TCTCAATGAG AAGTACGAAC ATGCTATTCG GGACATGGCA 
CGTGACTTGG AGTGTTACAA GAACACTGTC GACTCACTAA CCAAGAAACA GGAGAACTAT 
GGAGCATTGT TTGATCTTTT TGAGCAAAAG CTTAGAAAAC TCACTCAACA CATTGATCGA 
TCCAACTTGA AGCCTGAAGA GGCAATACGA TTCAGGCAGG ACATTGCTCA TTTGAGGGAT 
ATTAGCAATC ATCTTGCATC CAACTCAGCT CATGCTAACG AAGGCGCTGG TGAGCTTCTT 
CGTCAACCAT CTCTGGAATC AGTTGCATCC CATCGATCAT CGATGTCATC GTCGTCGAAA 
AGCAGCAAGC AGGAGAAGAT CAGCTTGAGC TCGTTTGGCA AGAACAAGAA GAGCTGGATC 
CGCTCCTCAC TCTCCAAGTT CACCAAGAAG AAGAACAAGA ACTACGACGA AGCACATATG 
CCATCAATTT CCGGATCTCA AGGAACTCTT GACAACATTG ATGTGATTGA GTTGAAGCAA 
GAGCTCAAAG AACGCGATAG TGCACTTTAC GAAGTCCGCC TTGACAATCT GGATCGTGCC 
CGCGAAGTTG ATGTTCTGAG GGAGACAGTG AACAAGTTGA AAACCGAGAA CAAGCAATTA 
AAGAAAGAAG TGGACAAACT CACCAACGGT CCAGCCACTC GTGCTTCTTC CCGCGCCTCA 
ATTCCAGTTA TCTACGACGA TGAGCATGTC TATGATGCAG CGTGTAGCAG TACATCAGCT 
AGTCAATCTT CGAAACGATC CTCTGGCTGC AACTCAATCA AGGTTACTGT AAACGTGGAC 
ATCGCTGGAG AAATCAGTTC GATCGTTAAC CCGGACAAAG AGATAATCGT AGGATATCTT 
GCCATGCCAA CCAGTCAGTC ATGCTGGAAA GACATTGATG TTTCTATTCT AGGACTATTT 
GAAGTCTACC TATCCAGAAT TGATGTGGAG CATCAACTTG GAATCGATGC TCGTGATTCT 
ATCCTTGGCT ATCAAATTGG TGAACTTCGA CGCGTCATTG GAGACTCCAC AACCATGATA 
ACCAGCCATC CAACTGACAT TCTTACTTCC TCAACTACAA TCCGAATGTT CATGCACGGT 
GCCGCACAGA GTCGCGTAGA CAGTCTGGTC CTTGATATGC TTCTTCCAAA GCAAATGATT 
CTCCAACTCG TCAAGTCAAT TTTGACAGAG AGACGTCTGG TGTTAGCTGG AGCAACTGGA 
ATTGGAAAGA GCAAACTGGC GAAGACCCTG GCTGCTTATG TATCTATTCG AACAAATCAA 
TCCGAAGATA GTATTGTTAA TATCAGCATT CCTGAAAACA ATAAAGAAGA ATTGCTTCAA 
GTGGAACGAC GCCTGGAAAA GATCTTGAGA AGCAAAGAAT CATGCATCGT AATTCTAGAT 
AATATCCCAA AGAATCGAAT TGCATTTGTT GTATCCGTTT TTGCAAATGT CCCACTTCAA 
AACAACGAAG GTCCATTTGT AGTATGCACA GTCAACCGAT ATCAAATCCC TGAGCTTCAA 
ATTCACCACA ATTTCAAAAT GTCAGTAATG TCGAATCGTC TCGAAGGATT CATCCTACGT 
TACCTCCGAC GACGGGCGGT AGAGGATGAG TATCGTCTAA CTGTACAGAT GCCATCAGAG 
CTCTTCAAAA TCATTGACTT CTTCCCAATA GCTCTTCAGG CCGTCAATAA TTTTATTGAG 
AAAACGAATT CTGTTGATGT GACAGTTGGT CCAAGAGCAT GCTTGAACTG TCCTCTAACT 
GTCGATGGAT CCCGTGAATG GTTCATTCGA TTGTGGAATG AGAACTTCAT TCCATATTTG 
GAACGTGTTG CTAGAGATGG CAAAAAAACC TTCGGTCGCT GCACTTCCTT CGAGGATCCC 
ACCGACATCG TCTCTGAAAA ATGGCCGTGG TTCGATGGTG AAAACCCGGA GAATGTGCTC 
AAACGTCTTC AACTCCAAGA CCTCGTCCCG TCACCTGCCA ACTCATCCCG ACAACACTTC 
AATCCCCTCG AGTCGTTGAT CCAATTGCAT GCTACCAAGC ATCAGACCAT CGACAACATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001022171.1
CDS	1..4563
Translation	MPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGQFHSLDRKCHLQEFTSTEHRMAALLSPRRVPNSMSKYDSSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001027000.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001027000.3

ORF Insert Sequence:

ATGCCACCCG CGGTTTCTAA ATTACCCTCG CCACGTGTCG CCACGTCAGC AACCGCTTCA 
GCAACTAACC CAAATTCCAA CTTTCCACAA ATGTCAACAT CCAGGCTTCA GACTCCACAG 
TCAAGAATAT CGAAAATTGA TTCATCAAAG ATTGGTATCA AGCCAAAGAC GTCTGGACTT 
AAACCACCCT CATCATCAAC CACTTCATCA AATAATACAA ATTCATTCCG TCCGTCGAGC 
CGTTCGAGTG GCAATAATAA TGTTGGCTCG ACGATATCCA CATCTGCGAA GAGCTTAGAA 
TCATCATCAA CGTACAGCTC TATTTCGAAT CTAAACCGAC CTACCTCCCA ACTCCAAAAA 
CCTTCTAGAC CACAAACCCA GCTAGTTCGT GTTGCTACAA CTACAAAAAT CGGAAGCTCA 
AAGCTAGCCG CTCCGAAAGC CGTGAGCACC CCAAAACTTG CTTCTGTGAA GACTATTGGA 
GCAAAACAAG AGCCCGATAA CAGCGGTGGT GGTGGTGGTG GAATGCTGAA ATTAAAGTTA 
TTCAGTAGCA AAAACCCATC TTCCTCATCG AATAGCCCAC AACCTACGAG AAAGGCGGCG 
GCGGTGCCTC AACAACAAAC TTTGTCGAAA ATCGCTGCCC CAGTGAAAAG TGGCCTGAAG 
CCGCCGACCA GTAAGCTGGG AAGTGCCACG TCTATGTCGA AGCTTTGTAC GCCAAAAGTT 
TCCTACCGTA AAACGGACGC CCCAATCATA TCTCAACAAG ACTCGAAACG ATGCTCAAAG 
AGCAGTGAAG AAGAGTCCGG ATACGCTGGA TTCAACAGCA CGTCGCCAAC GTCATCATCG 
ACGGAAGGTT CCCTAAGCAT GCATTCCACA TCTTCCAAGA GTTCAACGTC AGACGAAAAG 
TCTCCGTCAT CAGACGATCT TACTCTTAAC GCCTCCATCG TGACAGCTAT CAGACAGCCG 
ATAGCCGCAA CACCGGTTTC TCCAAATATT ATCAACAAGC CTGTTGAGGA AAAACCAACA 
CTGGCAGTGA AAGGAGTGAA AAGCACAGCG AAAAAAGATC CACCTCCAGC TGTTCCGCCA 
CGTGACACCC AGCCAACAAT CGGAGTTGTT AGTCCAATTA TGGCACATAA GAAGTTGACA 
AATGACCCCG TGATATCTGA AAAACCAGAA CCTGAAAAGC TCCAATCAAT GAGCATCGAC 
ACGACGGACG TTCCACCGCT TCCACCTCTA AAATCAGTTG TTCCACTTAA AATGACTTCA 
ATCCGACAAC CACCAACGTA CGATGTTCTT CTAAAACAAG GAAAAATCAC ATCGCCTGTC 
AAGTCGTTTG GATATGAGCA GTCGTCCGCG TCTGAAGACT CCATTGTGGC TCATGCGTCG 
GCTCAGGTGA CTCCGCCGAC AAAAACTTCT GGTAATCATT CGCTGGAGAG AAGGATGGGA 
AAGAATAAGA CATCAGAATC CAGCGGCTAC ACCTCTGACG CCGGTGTTGC GATGTGCGCC 
AAAATGAGGG AGAAGCTGAA AGAATACGAT GACATGACTC GTCGAGCACA GAACGGCTAT 
CCTGACAACT TCGAAGACAG TTCCTCCTTG TCGTCTGGAA TATCCGATAA CAACGAGCTC 
GACGACATAT CCACGGACGA TTTGTCCGGA GTAGACATGG CAACAGTCGC CTCCAAACAT 
AGCGACTATT CCCACTTTGT TCGCCATCCC ACGTCTTCTT CCTCAAAGCC CCGAGTCCCC 
AGTCGGTCCT CCACATCAGT CGATTCTCGA TCTCGAGCAG AACAGGAGAA TGTGTACAAA 
CTTCTGTCCC AGTGCCGAAC GAGCCAACGT GGCGCCGCTG CCACCTCAAC CTTCGGACAA 
CATTCGCTAA GATCCCCGGG ATACTCATCC TATTCTCCAC ACTTATCAGT GTCAGCTGAT 
AAGGACACAA TGTCTATGCA CTCACAGACT AGTCGACGAC CTTCTTCACA AAAACCAAGC 
TATTCAGGCC AATTTCATTC ACTTGATCGT AAATGCCACC TTCAAGAGTT CACATCCACC 
GAGCACAGAA TGGCGGCTCT CTTGAGCCCG AGACGGGTGC CGAACTCGAT GTCGAAATAT 
GATTCTTCAG CAGCCGCCCT CAACGCTTCG GGTATGTCTC GTTCAATGAT CCTTCTCGAA 
TCCCTTTCAC CACGACCACC TCGACGTCAC CAATCACCGG CTGACTCGTG CATTATCACA 
GCTTCTCCAT CAGCCCCACG AAGATCACAT TCGCCACGTG GCCCCACCGC CCGTATCCCA 
CTTTCTCTCG CTTCTTCGCC TGTCCACGTC AATAACAATT GGGGATCCTA CTCGGCGCGT 
TCCCGAGGTG GAAGCTCTAC TGGTATCTAT GGAGAGACGT TCCAACTGCA CAGACTATCC 
GATGAAAAAT CCCCCGCACA TTCTGCCAAA AGTGAGATGG GATCCCAACT ATCACTGGCT 
AGCACGACAG CATATGGATC TCTCAATGAG AAGTACGAAC ATGCTATTCG GGACATGGCA 
CGTGACTTGG AGTGTTACAA GAACACTGTC GACTCACTAA CCAAGAAACA GGAGAACTAT 
GGAGCATTGT TTGATCTTTT TGAGCAAAAG CTTAGAAAAC TCACTCAACA CATTGATCGA 
TCCAACTTGA AGCCTGAAGA GGCAATACGA TTCAGGCAGG ACATTGCTCA TTTGAGGGAT 
ATTAGCAATC ATCTTGCATC CAACTCAGCT CATGCTAACG AAGGCGCTGG TGAGCTTCTT 
CGTCAACCAT CTCTGGAATC AGTTGCATCC CATCGATCAT CGATGTCATC GTCGTCGAAA 
AGCAGCAAGC AGGAGAAGAT CAGCTTGAGC TCGTTTGGCA AGAACAAGAA GAGCTGGATC 
CGCTCCTCAC TCTCCAAGTT CACCAAGAAG AAGAACAAGA ACTACGACGA AGCACATATG 
CCATCAATTT CCGGATCTCA AGGAACTCTT GACAACATTG ATGTGATTGA GTTGAAGCAA 
GAGCTCAAAG AACGCGATAG TGCACTTTAC GAAGTCCGCC TTGACAATCT GGATCGTGCC 
CGCGAAGTTG ATGTTCTGAG GGAGACAGTG AACAAGTTGA AAACCGAGAA CAAGCAATTA 
AAGAAAGAAG TGGACAAACT CACCAACGGT CCAGCCACTC GTGCTTCTTC CCGCGCCTCA 
ATTCCAGTTA TCTACGACGA TGAGCATGTC TATGATGCAG CGTGTAGCAG TACATCAGCT 
AGTCAATCTT CGAAACGATC CTCTGGCTGC AACTCAATCA AGGTTACTGT AAACGTGGAC 
ATCGCTGGAG AAATCAGTTC GATCGTTAAC CCGGACAAAG AGATAATCGT AGGATATCTT 
GCCATGCCAA CCAGTCAGTC ATGCTGGAAA GACATTGATG TTTCTATTCT AGGACTATTT 
GAAGTCTACC TATCCAGAAT TGATGTGGAG CATCAACTTG GAATCGATGC TCGTGATTCT 
ATCCTTGGCT ATCAAATTGG TGAACTTCGA CGCGTCATTG GAGACTCCAC AACCATGATA 
ACCAGCCATC CAACTGACAT TCTTACTTCC TCAACTACAA TCCGAATGTT CATGCACGGT 
GCCGCACAGA GTCGCGTAGA CAGTCTGGTC CTTGATATGC TTCTTCCAAA GCAAATGATT 
CTCCAACTCG TCAAGTCAAT TTTGACAGAG AGACGTCTGG TGTTAGCTGG AGCAACTGGA 
ATTGGAAAGA GCAAACTGGC GAAGACCCTG GCTGCTTATG TATCTATTCG AACAAATCAA 
TCCGAAGATA GTATTGTTAA TATCAGCATT CCTGAAAACA ATAAAGAAGA ATTGCTTCAA 
GTGGAACGAC GCCTGGAAAA GATCTTGAGA AGCAAAGAAT CATGCATCGT AATTCTAGAT 
AATATCCCAA AGAATCGAAT TGCATTTGTT GTATCCGTTT TTGCAAATGT CCCACTTCAA 
AACAACGAAG GTCCATTTGT AGTATGCACA GTCAACCGAT ATCAAATCCC TGAGCTTCAA 
ATTCACCACA ATTTCAAAAT GTCAGTAATG TCGAATCGTC TCGAAGGATT CATCCTACGT 
TACCTCCGAC GACGGGCGGT AGAGGATGAG TATCGTCTAA CTGTACAGAT GCCATCAGAG 
CTCTTCAAAA TCATTGACTT CTTCCCAATA GCTCTTCAGG CCGTCAATAA TTTTATTGAG 
AAAACGAATT CTGTTGATGT GACAGTTGGT CCAAGAGCAT GCTTGAACTG TCCTCTAACT 
GTCGATGGAT CCCGTGAATG GTTCATTCGA TTGTGGAATG AGAACTTCAT TCCATATTTG 
GAACGTGTTG CTAGAGATGG CAAAAAAACC TTCGGTCGCT GCACTTCCTT CGAGGATCCC 
ACCGACATCG TCTCTGAAAA ATGGCCGTGG TTCGATGGTG AAAACCCGGA GAATGTGCTC 
AAACGTCTTC AACTCCAAGA CCTCGTCCCG TCACCTGCCA ACTCATCCCG ACAACACTTC 
AATCCCCTCG AGTCGTTGAT CCAATTGCAT GCTACCAAGC ATCAGACCAT CGACAACATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267342.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254271.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa351861
Clone ID Related Accession (Same CDS sequence)	NM_001267342.1 , NM_001267342.2
Accession Version	NM_001267342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGATCCGCT CCTCACTCTC CAAGTTCACC AAGAAGAAGA ACAAGAACTA CGACGAAGCA 
CATATGCCAT CAATTTCCGG ATCTCAAGGA ACTCTTGACA ACATTGATGT GATTGAGTTG 
AAGCAAGAGC TCAAAGAACG CGATAGTGCA CTTTACGAAG TCCGCCTTGA CAATCTGGAT 
CGTGCCCGCG AAGTTGATGT TCTGAGGGAG ACAGTGAACA AGTTGAAAAC CGAGAACAAG 
CAATTAAAGA AAGAAGTGGA CAAACTCACC AACGGTCCAG CCACTCGTGC TTCTTCCCGC 
GCCTCAATTC CAGTTATCTA CGACGATGAG CATGTCTATG ATGCAGCGTG TAGCAGTACA 
TCAGCTAGTC AATCTTCGAA ACGATCCTCT GGCTGCAACT CAATCAAGGT TACTGTAAAC 
GTGGACATCG CTGGAGAAAT CAGTTCGATC GTTAACCCGG ACAAAGAGAT AATCGTAGGA 
TATCTTGCCA TGCCAACCAG TCAGTCATGC TGGAAAGACA TTGATGTTTC TATTCTAGGA 
CTATTTGAAG TCTACCTATC CAGAATTGAT GTGGAGCATC AACTTGGAAT CGATGCTCGT 
GATTCTATCC TTGGCTATCA AATTGGTGAA CTTCGACGCG TCATTGGAGA CTCCACAACC 
ATGATAACCA GCCATCCAAC TGACATTCTT ACTTCCTCAA CTACAATCCG AATGTTCATG 
CACGGTGCCG CACAGAGTCG CGTAGACAGT CTGGTCCTTG ATATGCTTCT TCCAAAGCAA 
ATGATTCTCC AACTCGTCAA GTCAATTTTG ACAGAGAGAC GTCTGGTGTT AGCTGGAGCA 
ACTGGAATTG GAAAGAGCAA ACTGGCGAAG ACCCTGGCTG CTTATGTATC TATTCGAACA 
AATCAATCCG AAGATAGTAT TGTTAATATC AGCATTCCTG AAAACAATAA AGAAGAATTG 
CTTCAAGTGG AACGACGCCT GGAAAAGATC TTGAGAAGCA AAGAATCATG CATCGTAATT 
CTAGATAATA TCCCAAAGAA TCGAATTGCA TTTGTTGTAT CCGTTTTTGC AAATGTCCCA 
CTTCAAAACA ACGAAGGTCC ATTTGTAGTA TGCACAGTCA ACCGATATCA AATCCCTGAG 
CTTCAAATTC ACCACAATTT CAAAATGTCA GTAATGTCGA ATCGTCTCGA AGGATTCATC 
CTACGTTACC TCCGACGACG GGCGGTAGAG GATGAGTATC GTCTAACTGT ACAGATGCCA 
TCAGAGCTCT TCAAAATCAT TGACTTCTTC CCAATAGCTC TTCAGGCCGT CAATAATTTT 
ATTGAGAAAA CGAATTCTGT TGATGTGACA GTTGGTCCAA GAGCATGCTT GAACTGTCCT 
CTAACTGTCG ATGGATCCCG TGAATGGTTC ATTCGATTGT GGAATGAGAA CTTCATTCCA 
TATTTGGAAC GTGTTGCTAG AGATGGCAAA AAAACCTTCG GTCGCTGCAC TTCCTTCGAG 
GATCCCACCG ACATCGTCTC TGAAAAATGG CCGTGGTTCG ATGGTGAAAA CCCGGAGAAT 
GTGCTCAAAC GTCTTCAACT CCAAGACCTC GTCCCGTCAC CTGCCAACTC ATCCCGACAA 
CACTTCAATC CCCTCGAGTC GTTGATCCAA TTGCATGCTA CCAAGCATCA GACCATCGAC 
AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254271.1
CDS	1..4629
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267342.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267342.1

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGATCCGCT CCTCACTCTC CAAGTTCACC AAGAAGAAGA ACAAGAACTA CGACGAAGCA 
CATATGCCAT CAATTTCCGG ATCTCAAGGA ACTCTTGACA ACATTGATGT GATTGAGTTG 
AAGCAAGAGC TCAAAGAACG CGATAGTGCA CTTTACGAAG TCCGCCTTGA CAATCTGGAT 
CGTGCCCGCG AAGTTGATGT TCTGAGGGAG ACAGTGAACA AGTTGAAAAC CGAGAACAAG 
CAATTAAAGA AAGAAGTGGA CAAACTCACC AACGGTCCAG CCACTCGTGC TTCTTCCCGC 
GCCTCAATTC CAGTTATCTA CGACGATGAG CATGTCTATG ATGCAGCGTG TAGCAGTACA 
TCAGCTAGTC AATCTTCGAA ACGATCCTCT GGCTGCAACT CAATCAAGGT TACTGTAAAC 
GTGGACATCG CTGGAGAAAT CAGTTCGATC GTTAACCCGG ACAAAGAGAT AATCGTAGGA 
TATCTTGCCA TGCCAACCAG TCAGTCATGC TGGAAAGACA TTGATGTTTC TATTCTAGGA 
CTATTTGAAG TCTACCTATC CAGAATTGAT GTGGAGCATC AACTTGGAAT CGATGCTCGT 
GATTCTATCC TTGGCTATCA AATTGGTGAA CTTCGACGCG TCATTGGAGA CTCCACAACC 
ATGATAACCA GCCATCCAAC TGACATTCTT ACTTCCTCAA CTACAATCCG AATGTTCATG 
CACGGTGCCG CACAGAGTCG CGTAGACAGT CTGGTCCTTG ATATGCTTCT TCCAAAGCAA 
ATGATTCTCC AACTCGTCAA GTCAATTTTG ACAGAGAGAC GTCTGGTGTT AGCTGGAGCA 
ACTGGAATTG GAAAGAGCAA ACTGGCGAAG ACCCTGGCTG CTTATGTATC TATTCGAACA 
AATCAATCCG AAGATAGTAT TGTTAATATC AGCATTCCTG AAAACAATAA AGAAGAATTG 
CTTCAAGTGG AACGACGCCT GGAAAAGATC TTGAGAAGCA AAGAATCATG CATCGTAATT 
CTAGATAATA TCCCAAAGAA TCGAATTGCA TTTGTTGTAT CCGTTTTTGC AAATGTCCCA 
CTTCAAAACA ACGAAGGTCC ATTTGTAGTA TGCACAGTCA ACCGATATCA AATCCCTGAG 
CTTCAAATTC ACCACAATTT CAAAATGTCA GTAATGTCGA ATCGTCTCGA AGGATTCATC 
CTACGTTACC TCCGACGACG GGCGGTAGAG GATGAGTATC GTCTAACTGT ACAGATGCCA 
TCAGAGCTCT TCAAAATCAT TGACTTCTTC CCAATAGCTC TTCAGGCCGT CAATAATTTT 
ATTGAGAAAA CGAATTCTGT TGATGTGACA GTTGGTCCAA GAGCATGCTT GAACTGTCCT 
CTAACTGTCG ATGGATCCCG TGAATGGTTC ATTCGATTGT GGAATGAGAA CTTCATTCCA 
TATTTGGAAC GTGTTGCTAG AGATGGCAAA AAAACCTTCG GTCGCTGCAC TTCCTTCGAG 
GATCCCACCG ACATCGTCTC TGAAAAATGG CCGTGGTTCG ATGGTGAAAA CCCGGAGAAT 
GTGCTCAAAC GTCTTCAACT CCAAGACCTC GTCCCGTCAC CTGCCAACTC ATCCCGACAA 
CACTTCAATC CCCTCGAGTC GTTGATCCAA TTGCATGCTA CCAAGCATCA GACCATCGAC 
AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267342.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254271.1 Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.

Price & Availability↑

CloneID	OCa351861
Clone ID Related Accession (Same CDS sequence)	NM_001267342.1 , NM_001267342.2
Accession Version	NM_001267342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calponin-homology (CH) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267342.1.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGATCCGCT CCTCACTCTC CAAGTTCACC AAGAAGAAGA ACAAGAACTA CGACGAAGCA 
CATATGCCAT CAATTTCCGG ATCTCAAGGA ACTCTTGACA ACATTGATGT GATTGAGTTG 
AAGCAAGAGC TCAAAGAACG CGATAGTGCA CTTTACGAAG TCCGCCTTGA CAATCTGGAT 
CGTGCCCGCG AAGTTGATGT TCTGAGGGAG ACAGTGAACA AGTTGAAAAC CGAGAACAAG 
CAATTAAAGA AAGAAGTGGA CAAACTCACC AACGGTCCAG CCACTCGTGC TTCTTCCCGC 
GCCTCAATTC CAGTTATCTA CGACGATGAG CATGTCTATG ATGCAGCGTG TAGCAGTACA 
TCAGCTAGTC AATCTTCGAA ACGATCCTCT GGCTGCAACT CAATCAAGGT TACTGTAAAC 
GTGGACATCG CTGGAGAAAT CAGTTCGATC GTTAACCCGG ACAAAGAGAT AATCGTAGGA 
TATCTTGCCA TGCCAACCAG TCAGTCATGC TGGAAAGACA TTGATGTTTC TATTCTAGGA 
CTATTTGAAG TCTACCTATC CAGAATTGAT GTGGAGCATC AACTTGGAAT CGATGCTCGT 
GATTCTATCC TTGGCTATCA AATTGGTGAA CTTCGACGCG TCATTGGAGA CTCCACAACC 
ATGATAACCA GCCATCCAAC TGACATTCTT ACTTCCTCAA CTACAATCCG AATGTTCATG 
CACGGTGCCG CACAGAGTCG CGTAGACAGT CTGGTCCTTG ATATGCTTCT TCCAAAGCAA 
ATGATTCTCC AACTCGTCAA GTCAATTTTG ACAGAGAGAC GTCTGGTGTT AGCTGGAGCA 
ACTGGAATTG GAAAGAGCAA ACTGGCGAAG ACCCTGGCTG CTTATGTATC TATTCGAACA 
AATCAATCCG AAGATAGTAT TGTTAATATC AGCATTCCTG AAAACAATAA AGAAGAATTG 
CTTCAAGTGG AACGACGCCT GGAAAAGATC TTGAGAAGCA AAGAATCATG CATCGTAATT 
CTAGATAATA TCCCAAAGAA TCGAATTGCA TTTGTTGTAT CCGTTTTTGC AAATGTCCCA 
CTTCAAAACA ACGAAGGTCC ATTTGTAGTA TGCACAGTCA ACCGATATCA AATCCCTGAG 
CTTCAAATTC ACCACAATTT CAAAATGTCA GTAATGTCGA ATCGTCTCGA AGGATTCATC 
CTACGTTACC TCCGACGACG GGCGGTAGAG GATGAGTATC GTCTAACTGT ACAGATGCCA 
TCAGAGCTCT TCAAAATCAT TGACTTCTTC CCAATAGCTC TTCAGGCCGT CAATAATTTT 
ATTGAGAAAA CGAATTCTGT TGATGTGACA GTTGGTCCAA GAGCATGCTT GAACTGTCCT 
CTAACTGTCG ATGGATCCCG TGAATGGTTC ATTCGATTGT GGAATGAGAA CTTCATTCCA 
TATTTGGAAC GTGTTGCTAG AGATGGCAAA AAAACCTTCG GTCGCTGCAC TTCCTTCGAG 
GATCCCACCG ACATCGTCTC TGAAAAATGG CCGTGGTTCG ATGGTGAAAA CCCGGAGAAT 
GTGCTCAAAC GTCTTCAACT CCAAGACCTC GTCCCGTCAC CTGCCAACTC ATCCCGACAA 
CACTTCAATC CCCTCGAGTC GTTGATCCAA TTGCATGCTA CCAAGCATCA GACCATCGAC 
AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254271.1
CDS	42..4670
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267342.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267342.2

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGG ATCCTACTCG 
GCGCGTTCCC GAGGTGGAAG CTCTACTGGT ATCTATGGAG AGACGTTCCA ACTGCACAGA 
CTATCCGATG AAAAATCCCC CGCACATTCT GCCAAAAGTG AGATGGGATC CCAACTATCA 
CTGGCTAGCA CGACAGCATA TGGATCTCTC AATGAGAAGT ACGAACATGC TATTCGGGAC 
ATGGCACGTG ACTTGGAGTG TTACAAGAAC ACTGTCGACT CACTAACCAA GAAACAGGAG 
AACTATGGAG CATTGTTTGA TCTTTTTGAG CAAAAGCTTA GAAAACTCAC TCAACACATT 
GATCGATCCA ACTTGAAGCC TGAAGAGGCA ATACGATTCA GGCAGGACAT TGCTCATTTG 
AGGGATATTA GCAATCATCT TGCATCCAAC TCAGCTCATG CTAACGAAGG CGCTGGTGAG 
CTTCTTCGTC AACCATCTCT GGAATCAGTT GCATCCCATC GATCATCGAT GTCATCGTCG 
TCGAAAAGCA GCAAGCAGGA GAAGATCAGC TTGAGCTCGT TTGGCAAGAA CAAGAAGAGC 
TGGATCCGCT CCTCACTCTC CAAGTTCACC AAGAAGAAGA ACAAGAACTA CGACGAAGCA 
CATATGCCAT CAATTTCCGG ATCTCAAGGA ACTCTTGACA ACATTGATGT GATTGAGTTG 
AAGCAAGAGC TCAAAGAACG CGATAGTGCA CTTTACGAAG TCCGCCTTGA CAATCTGGAT 
CGTGCCCGCG AAGTTGATGT TCTGAGGGAG ACAGTGAACA AGTTGAAAAC CGAGAACAAG 
CAATTAAAGA AAGAAGTGGA CAAACTCACC AACGGTCCAG CCACTCGTGC TTCTTCCCGC 
GCCTCAATTC CAGTTATCTA CGACGATGAG CATGTCTATG ATGCAGCGTG TAGCAGTACA 
TCAGCTAGTC AATCTTCGAA ACGATCCTCT GGCTGCAACT CAATCAAGGT TACTGTAAAC 
GTGGACATCG CTGGAGAAAT CAGTTCGATC GTTAACCCGG ACAAAGAGAT AATCGTAGGA 
TATCTTGCCA TGCCAACCAG TCAGTCATGC TGGAAAGACA TTGATGTTTC TATTCTAGGA 
CTATTTGAAG TCTACCTATC CAGAATTGAT GTGGAGCATC AACTTGGAAT CGATGCTCGT 
GATTCTATCC TTGGCTATCA AATTGGTGAA CTTCGACGCG TCATTGGAGA CTCCACAACC 
ATGATAACCA GCCATCCAAC TGACATTCTT ACTTCCTCAA CTACAATCCG AATGTTCATG 
CACGGTGCCG CACAGAGTCG CGTAGACAGT CTGGTCCTTG ATATGCTTCT TCCAAAGCAA 
ATGATTCTCC AACTCGTCAA GTCAATTTTG ACAGAGAGAC GTCTGGTGTT AGCTGGAGCA 
ACTGGAATTG GAAAGAGCAA ACTGGCGAAG ACCCTGGCTG CTTATGTATC TATTCGAACA 
AATCAATCCG AAGATAGTAT TGTTAATATC AGCATTCCTG AAAACAATAA AGAAGAATTG 
CTTCAAGTGG AACGACGCCT GGAAAAGATC TTGAGAAGCA AAGAATCATG CATCGTAATT 
CTAGATAATA TCCCAAAGAA TCGAATTGCA TTTGTTGTAT CCGTTTTTGC AAATGTCCCA 
CTTCAAAACA ACGAAGGTCC ATTTGTAGTA TGCACAGTCA ACCGATATCA AATCCCTGAG 
CTTCAAATTC ACCACAATTT CAAAATGTCA GTAATGTCGA ATCGTCTCGA AGGATTCATC 
CTACGTTACC TCCGACGACG GGCGGTAGAG GATGAGTATC GTCTAACTGT ACAGATGCCA 
TCAGAGCTCT TCAAAATCAT TGACTTCTTC CCAATAGCTC TTCAGGCCGT CAATAATTTT 
ATTGAGAAAA CGAATTCTGT TGATGTGACA GTTGGTCCAA GAGCATGCTT GAACTGTCCT 
CTAACTGTCG ATGGATCCCG TGAATGGTTC ATTCGATTGT GGAATGAGAA CTTCATTCCA 
TATTTGGAAC GTGTTGCTAG AGATGGCAAA AAAACCTTCG GTCGCTGCAC TTCCTTCGAG 
GATCCCACCG ACATCGTCTC TGAAAAATGG CCGTGGTTCG ATGGTGAAAA CCCGGAGAAT 
GTGCTCAAAC GTCTTCAACT CCAAGACCTC GTCCCGTCAC CTGCCAACTC ATCCCGACAA 
CACTTCAATC CCCTCGAGTC GTTGATCCAA TTGCATGCTA CCAAGCATCA GACCATCGAC 
AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267343.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254272.1 Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.

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CloneID	OCa351862
Clone ID Related Accession (Same CDS sequence)	NM_001267343.2 , NM_001267343.1
Accession Version	NM_001267343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calponin-homology (CH) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001267343.1.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGC AGCCGCCCTC 
AACGCTTCGG GTATGTCTCG TTCAATGATC CTTCTCGAAT CCCTTTCACC ACGACCACCT 
CGACGTCACC AATCACCGGC TGACTCGTGC ATTATCACAG CTTCTCCATC AGCCCCACGA 
AGATCACATT CGCCACGTGG CCCCACCGCC CGTATCCCAC TTTCTCTCGC TTCTTCGCCT 
GTCCACGTCA ATAACAATTG GGGATCCTAC TCGGCGCGTT CCCGAGGTGG AAGCTCTACT 
GGTATCTATG GAGAGACGTT CCAACTGCAC AGACTATCCG ATGAAAAATC CCCCGCACAT 
TCTGCCAAAA GTGAGATGGG ATCCCAACTA TCACTGGCTA GCACGACAGC ATATGGATCT 
CTCAATGAGA AGTACGAACA TGCTATTCGG GACATGGCAC GTGACTTGGA GTGTTACAAG 
AACACTGTCG ACTCACTAAC CAAGAAACAG GAGAACTATG GAGCATTGTT TGATCTTTTT 
GAGCAAAAGC TTAGAAAACT CACTCAACAC ATTGATCGAT CCAACTTGAA GCCTGAAGAG 
GCAATACGAT TCAGGCAGGA CATTGCTCAT TTGAGGGATA TTAGCAATCA TCTTGCATCC 
AACTCAGCTC ATGCTAACGA AGGCGCTGGT GAGCTTCTTC GTCAACCATC TCTGGAATCA 
GTTGCATCCC ATCGATCATC GATGTCATCG TCGTCGAAAA GCAGCAAGCA GGAGAAGATC 
AGCTTGAGCT CGTTTGGCAA GAACAAGAAG AGCTGGATCC GCTCCTCACT CTCCAAGTTC 
ACCAAGAAGA AGAACAAGAA CTACGACGAA GCACATATGC CATCAATTTC CGGATCTCAA 
GGAACTCTTG ACAACATTGA TGTGATTGAG TTGAAGCAAG AGCTCAAAGA ACGCGATAGT 
GCACTTTACG AAGTCCGCCT TGACAATCTG GATCGTGCCC GCGAAGTTGA TGTTCTGAGG 
GAGACAGTGA ACAAGTTGAA AACCGAGAAC AAGCAATTAA AGAAAGAAGT GGACAAACTC 
ACCAACGGTC CAGCCACTCG TGCTTCTTCC CGCGCCTCAA TTCCAGTTAT CTACGACGAT 
GAGCATGTCT ATGATGCAGC GTGTAGCAGT ACATCAGCTA GTCAATCTTC GAAACGATCC 
TCTGGCTGCA ACTCAATCAA GGTTACTGTA AACGTGGACA TCGCTGGAGA AATCAGTTCG 
ATCGTTAACC CGGACAAAGA GATAATCGTA GGATATCTTG CCATGCCAAC CAGTCAGTCA 
TGCTGGAAAG ACATTGATGT TTCTATTCTA GGACTATTTG AAGTCTACCT ATCCAGAATT 
GATGTGGAGC ATCAACTTGG AATCGATGCT CGTGATTCTA TCCTTGGCTA TCAAATTGGT 
GAACTTCGAC GCGTCATTGG AGACTCCACA ACCATGATAA CCAGCCATCC AACTGACATT 
CTTACTTCCT CAACTACAAT CCGAATGTTC ATGCACGGTG CCGCACAGAG TCGCGTAGAC 
AGTCTGGTCC TTGATATGCT TCTTCCAAAG CAAATGATTC TCCAACTCGT CAAGTCAATT 
TTGACAGAGA GACGTCTGGT GTTAGCTGGA GCAACTGGAA TTGGAAAGAG CAAACTGGCG 
AAGACCCTGG CTGCTTATGT ATCTATTCGA ACAAATCAAT CCGAAGATAG TATTGTTAAT 
ATCAGCATTC CTGAAAACAA TAAAGAAGAA TTGCTTCAAG TGGAACGACG CCTGGAAAAG 
ATCTTGAGAA GCAAAGAATC ATGCATCGTA ATTCTAGATA ATATCCCAAA GAATCGAATT 
GCATTTGTTG TATCCGTTTT TGCAAATGTC CCACTTCAAA ACAACGAAGG TCCATTTGTA 
GTATGCACAG TCAACCGATA TCAAATCCCT GAGCTTCAAA TTCACCACAA TTTCAAAATG 
TCAGTAATGT CGAATCGTCT CGAAGGATTC ATCCTACGTT ACCTCCGACG ACGGGCGGTA 
GAGGATGAGT ATCGTCTAAC TGTACAGATG CCATCAGAGC TCTTCAAAAT CATTGACTTC 
TTCCCAATAG CTCTTCAGGC CGTCAATAAT TTTATTGAGA AAACGAATTC TGTTGATGTG 
ACAGTTGGTC CAAGAGCATG CTTGAACTGT CCTCTAACTG TCGATGGATC CCGTGAATGG 
TTCATTCGAT TGTGGAATGA GAACTTCATT CCATATTTGG AACGTGTTGC TAGAGATGGC 
AAAAAAACCT TCGGTCGCTG CACTTCCTTC GAGGATCCCA CCGACATCGT CTCTGAAAAA 
TGGCCGTGGT TCGATGGTGA AAACCCGGAG AATGTGCTCA AACGTCTTCA ACTCCAAGAC 
CTCGTCCCGT CACCTGCCAA CTCATCCCGA CAACACTTCA ATCCCCTCGA GTCGTTGATC 
CAATTGCATG CTACCAAGCA TCAGACCATC GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254272.1
CDS	42..4883
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267343.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (unc-53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267343.2

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGC AGCCGCCCTC 
AACGCTTCGG GTATGTCTCG TTCAATGATC CTTCTCGAAT CCCTTTCACC ACGACCACCT 
CGACGTCACC AATCACCGGC TGACTCGTGC ATTATCACAG CTTCTCCATC AGCCCCACGA 
AGATCACATT CGCCACGTGG CCCCACCGCC CGTATCCCAC TTTCTCTCGC TTCTTCGCCT 
GTCCACGTCA ATAACAATTG GGGATCCTAC TCGGCGCGTT CCCGAGGTGG AAGCTCTACT 
GGTATCTATG GAGAGACGTT CCAACTGCAC AGACTATCCG ATGAAAAATC CCCCGCACAT 
TCTGCCAAAA GTGAGATGGG ATCCCAACTA TCACTGGCTA GCACGACAGC ATATGGATCT 
CTCAATGAGA AGTACGAACA TGCTATTCGG GACATGGCAC GTGACTTGGA GTGTTACAAG 
AACACTGTCG ACTCACTAAC CAAGAAACAG GAGAACTATG GAGCATTGTT TGATCTTTTT 
GAGCAAAAGC TTAGAAAACT CACTCAACAC ATTGATCGAT CCAACTTGAA GCCTGAAGAG 
GCAATACGAT TCAGGCAGGA CATTGCTCAT TTGAGGGATA TTAGCAATCA TCTTGCATCC 
AACTCAGCTC ATGCTAACGA AGGCGCTGGT GAGCTTCTTC GTCAACCATC TCTGGAATCA 
GTTGCATCCC ATCGATCATC GATGTCATCG TCGTCGAAAA GCAGCAAGCA GGAGAAGATC 
AGCTTGAGCT CGTTTGGCAA GAACAAGAAG AGCTGGATCC GCTCCTCACT CTCCAAGTTC 
ACCAAGAAGA AGAACAAGAA CTACGACGAA GCACATATGC CATCAATTTC CGGATCTCAA 
GGAACTCTTG ACAACATTGA TGTGATTGAG TTGAAGCAAG AGCTCAAAGA ACGCGATAGT 
GCACTTTACG AAGTCCGCCT TGACAATCTG GATCGTGCCC GCGAAGTTGA TGTTCTGAGG 
GAGACAGTGA ACAAGTTGAA AACCGAGAAC AAGCAATTAA AGAAAGAAGT GGACAAACTC 
ACCAACGGTC CAGCCACTCG TGCTTCTTCC CGCGCCTCAA TTCCAGTTAT CTACGACGAT 
GAGCATGTCT ATGATGCAGC GTGTAGCAGT ACATCAGCTA GTCAATCTTC GAAACGATCC 
TCTGGCTGCA ACTCAATCAA GGTTACTGTA AACGTGGACA TCGCTGGAGA AATCAGTTCG 
ATCGTTAACC CGGACAAAGA GATAATCGTA GGATATCTTG CCATGCCAAC CAGTCAGTCA 
TGCTGGAAAG ACATTGATGT TTCTATTCTA GGACTATTTG AAGTCTACCT ATCCAGAATT 
GATGTGGAGC ATCAACTTGG AATCGATGCT CGTGATTCTA TCCTTGGCTA TCAAATTGGT 
GAACTTCGAC GCGTCATTGG AGACTCCACA ACCATGATAA CCAGCCATCC AACTGACATT 
CTTACTTCCT CAACTACAAT CCGAATGTTC ATGCACGGTG CCGCACAGAG TCGCGTAGAC 
AGTCTGGTCC TTGATATGCT TCTTCCAAAG CAAATGATTC TCCAACTCGT CAAGTCAATT 
TTGACAGAGA GACGTCTGGT GTTAGCTGGA GCAACTGGAA TTGGAAAGAG CAAACTGGCG 
AAGACCCTGG CTGCTTATGT ATCTATTCGA ACAAATCAAT CCGAAGATAG TATTGTTAAT 
ATCAGCATTC CTGAAAACAA TAAAGAAGAA TTGCTTCAAG TGGAACGACG CCTGGAAAAG 
ATCTTGAGAA GCAAAGAATC ATGCATCGTA ATTCTAGATA ATATCCCAAA GAATCGAATT 
GCATTTGTTG TATCCGTTTT TGCAAATGTC CCACTTCAAA ACAACGAAGG TCCATTTGTA 
GTATGCACAG TCAACCGATA TCAAATCCCT GAGCTTCAAA TTCACCACAA TTTCAAAATG 
TCAGTAATGT CGAATCGTCT CGAAGGATTC ATCCTACGTT ACCTCCGACG ACGGGCGGTA 
GAGGATGAGT ATCGTCTAAC TGTACAGATG CCATCAGAGC TCTTCAAAAT CATTGACTTC 
TTCCCAATAG CTCTTCAGGC CGTCAATAAT TTTATTGAGA AAACGAATTC TGTTGATGTG 
ACAGTTGGTC CAAGAGCATG CTTGAACTGT CCTCTAACTG TCGATGGATC CCGTGAATGG 
TTCATTCGAT TGTGGAATGA GAACTTCATT CCATATTTGG AACGTGTTGC TAGAGATGGC 
AAAAAAACCT TCGGTCGCTG CACTTCCTTC GAGGATCCCA CCGACATCGT CTCTGAAAAA 
TGGCCGTGGT TCGATGGTGA AAACCCGGAG AATGTGCTCA AACGTCTTCA ACTCCAAGAC 
CTCGTCCCGT CACCTGCCAA CTCATCCCGA CAACACTTCA ATCCCCTCGA GTCGTTGATC 
CAATTGCATG CTACCAAGCA TCAGACCATC GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-53 ( NM_001267343.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001254272.1 Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

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CloneID	OCa351862
Clone ID Related Accession (Same CDS sequence)	NM_001267343.2 , NM_001267343.1
Accession Version	NM_001267343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Adapter protein unc-53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGC AGCCGCCCTC 
AACGCTTCGG GTATGTCTCG TTCAATGATC CTTCTCGAAT CCCTTTCACC ACGACCACCT 
CGACGTCACC AATCACCGGC TGACTCGTGC ATTATCACAG CTTCTCCATC AGCCCCACGA 
AGATCACATT CGCCACGTGG CCCCACCGCC CGTATCCCAC TTTCTCTCGC TTCTTCGCCT 
GTCCACGTCA ATAACAATTG GGGATCCTAC TCGGCGCGTT CCCGAGGTGG AAGCTCTACT 
GGTATCTATG GAGAGACGTT CCAACTGCAC AGACTATCCG ATGAAAAATC CCCCGCACAT 
TCTGCCAAAA GTGAGATGGG ATCCCAACTA TCACTGGCTA GCACGACAGC ATATGGATCT 
CTCAATGAGA AGTACGAACA TGCTATTCGG GACATGGCAC GTGACTTGGA GTGTTACAAG 
AACACTGTCG ACTCACTAAC CAAGAAACAG GAGAACTATG GAGCATTGTT TGATCTTTTT 
GAGCAAAAGC TTAGAAAACT CACTCAACAC ATTGATCGAT CCAACTTGAA GCCTGAAGAG 
GCAATACGAT TCAGGCAGGA CATTGCTCAT TTGAGGGATA TTAGCAATCA TCTTGCATCC 
AACTCAGCTC ATGCTAACGA AGGCGCTGGT GAGCTTCTTC GTCAACCATC TCTGGAATCA 
GTTGCATCCC ATCGATCATC GATGTCATCG TCGTCGAAAA GCAGCAAGCA GGAGAAGATC 
AGCTTGAGCT CGTTTGGCAA GAACAAGAAG AGCTGGATCC GCTCCTCACT CTCCAAGTTC 
ACCAAGAAGA AGAACAAGAA CTACGACGAA GCACATATGC CATCAATTTC CGGATCTCAA 
GGAACTCTTG ACAACATTGA TGTGATTGAG TTGAAGCAAG AGCTCAAAGA ACGCGATAGT 
GCACTTTACG AAGTCCGCCT TGACAATCTG GATCGTGCCC GCGAAGTTGA TGTTCTGAGG 
GAGACAGTGA ACAAGTTGAA AACCGAGAAC AAGCAATTAA AGAAAGAAGT GGACAAACTC 
ACCAACGGTC CAGCCACTCG TGCTTCTTCC CGCGCCTCAA TTCCAGTTAT CTACGACGAT 
GAGCATGTCT ATGATGCAGC GTGTAGCAGT ACATCAGCTA GTCAATCTTC GAAACGATCC 
TCTGGCTGCA ACTCAATCAA GGTTACTGTA AACGTGGACA TCGCTGGAGA AATCAGTTCG 
ATCGTTAACC CGGACAAAGA GATAATCGTA GGATATCTTG CCATGCCAAC CAGTCAGTCA 
TGCTGGAAAG ACATTGATGT TTCTATTCTA GGACTATTTG AAGTCTACCT ATCCAGAATT 
GATGTGGAGC ATCAACTTGG AATCGATGCT CGTGATTCTA TCCTTGGCTA TCAAATTGGT 
GAACTTCGAC GCGTCATTGG AGACTCCACA ACCATGATAA CCAGCCATCC AACTGACATT 
CTTACTTCCT CAACTACAAT CCGAATGTTC ATGCACGGTG CCGCACAGAG TCGCGTAGAC 
AGTCTGGTCC TTGATATGCT TCTTCCAAAG CAAATGATTC TCCAACTCGT CAAGTCAATT 
TTGACAGAGA GACGTCTGGT GTTAGCTGGA GCAACTGGAA TTGGAAAGAG CAAACTGGCG 
AAGACCCTGG CTGCTTATGT ATCTATTCGA ACAAATCAAT CCGAAGATAG TATTGTTAAT 
ATCAGCATTC CTGAAAACAA TAAAGAAGAA TTGCTTCAAG TGGAACGACG CCTGGAAAAG 
ATCTTGAGAA GCAAAGAATC ATGCATCGTA ATTCTAGATA ATATCCCAAA GAATCGAATT 
GCATTTGTTG TATCCGTTTT TGCAAATGTC CCACTTCAAA ACAACGAAGG TCCATTTGTA 
GTATGCACAG TCAACCGATA TCAAATCCCT GAGCTTCAAA TTCACCACAA TTTCAAAATG 
TCAGTAATGT CGAATCGTCT CGAAGGATTC ATCCTACGTT ACCTCCGACG ACGGGCGGTA 
GAGGATGAGT ATCGTCTAAC TGTACAGATG CCATCAGAGC TCTTCAAAAT CATTGACTTC 
TTCCCAATAG CTCTTCAGGC CGTCAATAAT TTTATTGAGA AAACGAATTC TGTTGATGTG 
ACAGTTGGTC CAAGAGCATG CTTGAACTGT CCTCTAACTG TCGATGGATC CCGTGAATGG 
TTCATTCGAT TGTGGAATGA GAACTTCATT CCATATTTGG AACGTGTTGC TAGAGATGGC 
AAAAAAACCT TCGGTCGCTG CACTTCCTTC GAGGATCCCA CCGACATCGT CTCTGAAAAA 
TGGCCGTGGT TCGATGGTGA AAACCCGGAG AATGTGCTCA AACGTCTTCA ACTCCAAGAC 
CTCGTCCCGT CACCTGCCAA CTCATCCCGA CAACACTTCA ATCCCCTCGA GTCGTTGATC 
CAATTGCATG CTACCAAGCA TCAGACCATC GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254272.1
CDS	1..4842
Translation	MTTSNVELIPIYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLETCLDYLKNLGLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKKDQKKLEQLPTSIMPPAVSKLPSPRVATSATASATNPNSNFPQMSTSRLQTPQSRISKIDSSKIGIKPKTSGLKPPSSSTTSSNNTNSFRPSSRSSGNNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIGSSKLAAPKAVSTPKLASVKTIGAKQEPDNSGGGGGGMLKLKLFSSKNPSSSSNSPQPTRKAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSSEEESGYAGFNSTSPTSSSTEGSLSMHSTSSKSSTSDEKSPSSDDLTLNASIVTAIRQPIAATPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPRDTQPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLPPLKSVVPLKMTSIRQPPTYDVLLKQGKITSPVKSFGYEQSSASEDSIVAHASAQVTPPTKTSGNHSLERRMGKNKTSESSGYTSDAGVAMCAKMREKLKEYDDMTRRAQNGYPDNFEDSSSLSSGISDNNELDDISTDDLSGVDMATVASKHSDYSHFVRHPTSSSSKPRVPSRSSTSVDSRSRAEQENVYKLLSQCRTSQRGAAATSTFGQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRPSSQKPSYSAAALNASGMSRSMILLESLSPRPPRRHQSPADSCIITASPSAPRRSHSPRGPTARIPLSLASSPVHVNNNWGSYSARSRGGSSTGIYGETFQLHRLSDEKSPAHSAKSEMGSQLSLASTTAYGSLNEKYEHAIRDMARDLECYKNTVDSLTKKQENYGALFDLFEQKLRKLTQHIDRSNLKPEEAIRFRQDIAHLRDISNHLASNSAHANEGAGELLRQPSLESVASHRSSMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNKNYDEAHMPSISGSQGTLDNIDVIELKQELKERDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATRASSRASIPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKVTVNVDIAGEISSIVNPDKEIIVGYLAMPTSQSCWKDIDVSILGLFEVYLSRIDVEHQLGIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSSTTIRMFMHGAAQSRVDSLVLDMLLPKQMILQLVKSILTERRLVLAGATGIGKSKLAKTLAAYVSIRTNQSEDSIVNISIPENNKEELLQVERRLEKILRSKESCIVILDNIPKNRIAFVVSVFANVPLQNNEGPFVVCTVNRYQIPELQIHHNFKMSVMSNRLEGFILRYLRRRAVEDEYRLTVQMPSELFKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARDGKKTFGRCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDLVPSPANSSRQHFNPLESLIQLHATKHQTIDNI

Target ORF information:

RefSeq Version	NM_001267343.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Adapter protein unc-53 (unc-53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267343.1

ORF Insert Sequence:

ATGACGACGT CAAATGTAGA ATTGATACCA ATCTACACGG ATTGGGCCAA TCGGCACCTT 
TCGAAGGGCA GCTTATCAAA GTCGATTAGG GATATTTCCA ATGATTTTCG CGACTATCGA 
CTGGTTTCTC AGCTTATTAA TGTGATCGTT CCGATCAACG AATTCTCGCC TGCATTCACG 
AAACGTTTGG CAAAAATCAC ATCGAACCTG GATGGCCTCG AAACGTGTCT CGACTACCTG 
AAAAATCTGG GTCTCGACTG CTCGAAACTC ACCAAAACCG ATATCGACAG CGGAAACTTG 
GGTGCAGTTC TCCAGCTGCT CTTCCTGCTC TCCACCTACA AGCAGAAGCT TCGGCAACTG 
AAAAAAGATC AGAAGAAATT GGAGCAACTA CCCACATCCA TTATGCCACC CGCGGTTTCT 
AAATTACCCT CGCCACGTGT CGCCACGTCA GCAACCGCTT CAGCAACTAA CCCAAATTCC 
AACTTTCCAC AAATGTCAAC ATCCAGGCTT CAGACTCCAC AGTCAAGAAT ATCGAAAATT 
GATTCATCAA AGATTGGTAT CAAGCCAAAG ACGTCTGGAC TTAAACCACC CTCATCATCA 
ACCACTTCAT CAAATAATAC AAATTCATTC CGTCCGTCGA GCCGTTCGAG TGGCAATAAT 
AATGTTGGCT CGACGATATC CACATCTGCG AAGAGCTTAG AATCATCATC AACGTACAGC 
TCTATTTCGA ATCTAAACCG ACCTACCTCC CAACTCCAAA AACCTTCTAG ACCACAAACC 
CAGCTAGTTC GTGTTGCTAC AACTACAAAA ATCGGAAGCT CAAAGCTAGC CGCTCCGAAA 
GCCGTGAGCA CCCCAAAACT TGCTTCTGTG AAGACTATTG GAGCAAAACA AGAGCCCGAT 
AACAGCGGTG GTGGTGGTGG TGGAATGCTG AAATTAAAGT TATTCAGTAG CAAAAACCCA 
TCTTCCTCAT CGAATAGCCC ACAACCTACG AGAAAGGCGG CGGCGGTGCC TCAACAACAA 
ACTTTGTCGA AAATCGCTGC CCCAGTGAAA AGTGGCCTGA AGCCGCCGAC CAGTAAGCTG 
GGAAGTGCCA CGTCTATGTC GAAGCTTTGT ACGCCAAAAG TTTCCTACCG TAAAACGGAC 
GCCCCAATCA TATCTCAACA AGACTCGAAA CGATGCTCAA AGAGCAGTGA AGAAGAGTCC 
GGATACGCTG GATTCAACAG CACGTCGCCA ACGTCATCAT CGACGGAAGG TTCCCTAAGC 
ATGCATTCCA CATCTTCCAA GAGTTCAACG TCAGACGAAA AGTCTCCGTC ATCAGACGAT 
CTTACTCTTA ACGCCTCCAT CGTGACAGCT ATCAGACAGC CGATAGCCGC AACACCGGTT 
TCTCCAAATA TTATCAACAA GCCTGTTGAG GAAAAACCAA CACTGGCAGT GAAAGGAGTG 
AAAAGCACAG CGAAAAAAGA TCCACCTCCA GCTGTTCCGC CACGTGACAC CCAGCCAACA 
ATCGGAGTTG TTAGTCCAAT TATGGCACAT AAGAAGTTGA CAAATGACCC CGTGATATCT 
GAAAAACCAG AACCTGAAAA GCTCCAATCA ATGAGCATCG ACACGACGGA CGTTCCACCG 
CTTCCACCTC TAAAATCAGT TGTTCCACTT AAAATGACTT CAATCCGACA ACCACCAACG 
TACGATGTTC TTCTAAAACA AGGAAAAATC ACATCGCCTG TCAAGTCGTT TGGATATGAG 
CAGTCGTCCG CGTCTGAAGA CTCCATTGTG GCTCATGCGT CGGCTCAGGT GACTCCGCCG 
ACAAAAACTT CTGGTAATCA TTCGCTGGAG AGAAGGATGG GAAAGAATAA GACATCAGAA 
TCCAGCGGCT ACACCTCTGA CGCCGGTGTT GCGATGTGCG CCAAAATGAG GGAGAAGCTG 
AAAGAATACG ATGACATGAC TCGTCGAGCA CAGAACGGCT ATCCTGACAA CTTCGAAGAC 
AGTTCCTCCT TGTCGTCTGG AATATCCGAT AACAACGAGC TCGACGACAT ATCCACGGAC 
GATTTGTCCG GAGTAGACAT GGCAACAGTC GCCTCCAAAC ATAGCGACTA TTCCCACTTT 
GTTCGCCATC CCACGTCTTC TTCCTCAAAG CCCCGAGTCC CCAGTCGGTC CTCCACATCA 
GTCGATTCTC GATCTCGAGC AGAACAGGAG AATGTGTACA AACTTCTGTC CCAGTGCCGA 
ACGAGCCAAC GTGGCGCCGC TGCCACCTCA ACCTTCGGAC AACATTCGCT AAGATCCCCG 
GGATACTCAT CCTATTCTCC ACACTTATCA GTGTCAGCTG ATAAGGACAC AATGTCTATG 
CACTCACAGA CTAGTCGACG ACCTTCTTCA CAAAAACCAA GCTATTCAGC AGCCGCCCTC 
AACGCTTCGG GTATGTCTCG TTCAATGATC CTTCTCGAAT CCCTTTCACC ACGACCACCT 
CGACGTCACC AATCACCGGC TGACTCGTGC ATTATCACAG CTTCTCCATC AGCCCCACGA 
AGATCACATT CGCCACGTGG CCCCACCGCC CGTATCCCAC TTTCTCTCGC TTCTTCGCCT 
GTCCACGTCA ATAACAATTG GGGATCCTAC TCGGCGCGTT CCCGAGGTGG AAGCTCTACT 
GGTATCTATG GAGAGACGTT CCAACTGCAC AGACTATCCG ATGAAAAATC CCCCGCACAT 
TCTGCCAAAA GTGAGATGGG ATCCCAACTA TCACTGGCTA GCACGACAGC ATATGGATCT 
CTCAATGAGA AGTACGAACA TGCTATTCGG GACATGGCAC GTGACTTGGA GTGTTACAAG 
AACACTGTCG ACTCACTAAC CAAGAAACAG GAGAACTATG GAGCATTGTT TGATCTTTTT 
GAGCAAAAGC TTAGAAAACT CACTCAACAC ATTGATCGAT CCAACTTGAA GCCTGAAGAG 
GCAATACGAT TCAGGCAGGA CATTGCTCAT TTGAGGGATA TTAGCAATCA TCTTGCATCC 
AACTCAGCTC ATGCTAACGA AGGCGCTGGT GAGCTTCTTC GTCAACCATC TCTGGAATCA 
GTTGCATCCC ATCGATCATC GATGTCATCG TCGTCGAAAA GCAGCAAGCA GGAGAAGATC 
AGCTTGAGCT CGTTTGGCAA GAACAAGAAG AGCTGGATCC GCTCCTCACT CTCCAAGTTC 
ACCAAGAAGA AGAACAAGAA CTACGACGAA GCACATATGC CATCAATTTC CGGATCTCAA 
GGAACTCTTG ACAACATTGA TGTGATTGAG TTGAAGCAAG AGCTCAAAGA ACGCGATAGT 
GCACTTTACG AAGTCCGCCT TGACAATCTG GATCGTGCCC GCGAAGTTGA TGTTCTGAGG 
GAGACAGTGA ACAAGTTGAA AACCGAGAAC AAGCAATTAA AGAAAGAAGT GGACAAACTC 
ACCAACGGTC CAGCCACTCG TGCTTCTTCC CGCGCCTCAA TTCCAGTTAT CTACGACGAT 
GAGCATGTCT ATGATGCAGC GTGTAGCAGT ACATCAGCTA GTCAATCTTC GAAACGATCC 
TCTGGCTGCA ACTCAATCAA GGTTACTGTA AACGTGGACA TCGCTGGAGA AATCAGTTCG 
ATCGTTAACC CGGACAAAGA GATAATCGTA GGATATCTTG CCATGCCAAC CAGTCAGTCA 
TGCTGGAAAG ACATTGATGT TTCTATTCTA GGACTATTTG AAGTCTACCT ATCCAGAATT 
GATGTGGAGC ATCAACTTGG AATCGATGCT CGTGATTCTA TCCTTGGCTA TCAAATTGGT 
GAACTTCGAC GCGTCATTGG AGACTCCACA ACCATGATAA CCAGCCATCC AACTGACATT 
CTTACTTCCT CAACTACAAT CCGAATGTTC ATGCACGGTG CCGCACAGAG TCGCGTAGAC 
AGTCTGGTCC TTGATATGCT TCTTCCAAAG CAAATGATTC TCCAACTCGT CAAGTCAATT 
TTGACAGAGA GACGTCTGGT GTTAGCTGGA GCAACTGGAA TTGGAAAGAG CAAACTGGCG 
AAGACCCTGG CTGCTTATGT ATCTATTCGA ACAAATCAAT CCGAAGATAG TATTGTTAAT 
ATCAGCATTC CTGAAAACAA TAAAGAAGAA TTGCTTCAAG TGGAACGACG CCTGGAAAAG 
ATCTTGAGAA GCAAAGAATC ATGCATCGTA ATTCTAGATA ATATCCCAAA GAATCGAATT 
GCATTTGTTG TATCCGTTTT TGCAAATGTC CCACTTCAAA ACAACGAAGG TCCATTTGTA 
GTATGCACAG TCAACCGATA TCAAATCCCT GAGCTTCAAA TTCACCACAA TTTCAAAATG 
TCAGTAATGT CGAATCGTCT CGAAGGATTC ATCCTACGTT ACCTCCGACG ACGGGCGGTA 
GAGGATGAGT ATCGTCTAAC TGTACAGATG CCATCAGAGC TCTTCAAAAT CATTGACTTC 
TTCCCAATAG CTCTTCAGGC CGTCAATAAT TTTATTGAGA AAACGAATTC TGTTGATGTG 
ACAGTTGGTC CAAGAGCATG CTTGAACTGT CCTCTAACTG TCGATGGATC CCGTGAATGG 
TTCATTCGAT TGTGGAATGA GAACTTCATT CCATATTTGG AACGTGTTGC TAGAGATGGC 
AAAAAAACCT TCGGTCGCTG CACTTCCTTC GAGGATCCCA CCGACATCGT CTCTGAAAAA 
TGGCCGTGGT TCGATGGTGA AAACCCGGAG AATGTGCTCA AACGTCTTCA ACTCCAAGAC 
CTCGTCCCGT CACCTGCCAA CTCATCCCGA CAACACTTCA ATCCCCTCGA GTCGTTGATC 
CAATTGCATG CTACCAAGCA TCAGACCATC GACAACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	unc-53
Entrez Gene ID	174693
Full Name	Adapter protein unc-53
Gene Type	protein-coding
Organism	Caenorhabditis elegans

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

unc-53 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

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