ptc-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ptc-3
Entrez Gene ID	173631
Full Name	Protein patched homolog 3
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001129178.2	NP_001122650.1	Protein patched homolog 3
NM_061983.7	NP_494384.2	Protein patched homolog 3
NM_061982.5	NP_494383.2	Protein patched homolog 3
NM_061983.6	NP_494384.2	Protein patched homolog 3
NM_001129178.1	NP_001122650.1	Protein patched homolog 3
NM_061982.6	NP_494383.2	Protein patched homolog 3

Pathways from BioSystems

Homologies

	PTCH1	XP_001111868.2
Canis lupus familiaris (dog)	PTCH1	XP_003638919.1
Rattus norvegicus (Norway rat)	Ptch1	NP_446018.1
Gallus gallus (chicken)	PTCH1	NP_990291.1
Caenorhabditis elegans (roundworm)	ptc-1	NP_495662.2
Danio rerio (zebrafish)	ptch1	XP_001922161.4
Caenorhabditis elegans (roundworm)	ptc-3	NP_494384.2
Xenopus tropicalis (tropical clawed frog)	ptch1	XP_002935019.2
Pan troglodytes (chimpanzee)	PTCH1	XP_003312241.1
Bos taurus (cattle)	PTCH1	NP_001192808.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011395	XP_317925.4
Caenorhabditis elegans (roundworm)	ptc-2	NP_495218.2
Mus musculus (house mouse)	Ptch1	NP_032983.1
Homo sapiens (human)	PTCH1	NP_000255.2
Drosophila melanogaster (fruit fly)	ptc	NP_523661.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Consistent with this pattern of expression, ptc-3(RNAi) reveals an additional postembryonic requirement for ptc-3 activity
Title: C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain.

The following ptc-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptc-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339378	NM_061983.7 Latest version!	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa339379	NM_061982.6 Latest version!	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa339381	NM_001129178.2 Latest version!	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCa339378	NM_061983.6	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa339381	NM_001129178.1	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa339379	NM_061982.5	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptc-3 ( NM_061983.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494384.2 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.

Price & Availability↑

CloneID	OCa339378
Clone ID Related Accession (Same CDS sequence)	NM_061983.7 , NM_061983.6
Accession Version	NM_061983.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_061983.6.

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA TGTCATCACG TGCCGAGTTG 
GCTGGCTACG AGAAGCAGGC CGACGAGTAT AAGCGTCACG AGCCGTGGTA CACCGTCGGA 
GGATTCCTCA ACAAGATCTA CATTCCGGCA CTCAAGAATA ATGTTGTTAA GGCTTGCGTT 
CTCATCGGAA CTACCACCGC CGTCGTCTTC GGACTCTACG GAATGTACAC CTCGACTCTC 
GGTCTCGAGC TCGCCGACGT GCTTCCAGAG CACACCCCAC CAGCGGCCTT CCTTCGTGCT 
CGCGAGCAAT ACTTCTCCTT CTACCCGATG TTTGCAGTTC TTCGTGGTGA CAAGCTTGAC 
ATTCCGAACC AACAGCAACT TATCGAAGAG TACAGAGCAC AGTTGGGATC TTCAAAGTTT 
ATGATCAAGG CTGAAGGTAA ACTTCAACCC TACTGGATGT CTATGCTCAG AGTCTGGCTG 
CAATCTTTGG ACATGGCCTT GGAAAAAGAC CTCGCCGCTG GCAAATTCGA TTTGACAAAT 
GGAAATCCTA TCAAGGTGAA CGGAGAGAAG CCAAGTCCCG AATCGATGAT CGCGGCTAGA 
CTTGTATGCT CCTTCGGCAC CAACTACAAC TGTGACGGGC GGCTCGGAAA GATGAAGATG 
GTGGAAAATG AGGTTATTAA TCCAGAAGGA TTCTATAACT ATCTGACGGG ATGGTTCAAC 
GTTGACAACA TGATGTACTA CGTGTCCCAG GCTTCATTCT ATCCAACACC ACCTGGATGG 
GAGTATAATG AGAAGCTCGC CAAGGTGGTT CCGGCTGCCG AGCCATTGTT GTACTCACAG 
ATGCCGTTTT ATCAGAATGA TTTGATCGAT ACACCAGCGA TTGTAAAAAT GATTGAGGAA 
ATCCGTGCCA CTTGCGAGGA ATACTCCGAA CGAGGCCTTT CCAACCATCC ATCGGGTATC 
GCCTTCACCT TCTGGGAGCA ATATCTTACT TTGAGATGGA ACTTGTTCCA AGCCATCTGC 
ATCATCGCGC TCGCCGTGTT CTGTGTCATT TCAATTCTAA TGTTCAATCC ATGGGCAGCC 
ACTCTTATCA TGTGCATCGT AGTGATCACT ACCATCGAGC TGGGAGGATT CATGGGGCTT 
ATGGGCATCA AGATGAACCC GATTTCCGCT GTAACCCTCA TCTGTGCCGT CGGAATCGGT 
GTCGAGTTCA CTGCTCACGT CGAGTTGGCC TTCCTGACAG CTCTAGGAAC CATCGATCAA 
CGCCTGGAGT CTTGCCTCCA GCACATGTTC GTGCCAGTCT ATCACGGAGC CATCTCAACG 
TTCCTCGGAG TTGTGATGCT TGTGTTCTCC GAGTTCGACT TCGTCGTCAC CTACTTCTTC 
TACACAATGA CGTTGCTGGT AGCGTTGGGT GTGTTCAACG GGTTGTGTGT TCTTCCAGTG 
ATCCTTACGC TGGTCGGCCC GAAGCCTGAG CTGACACCAA CCGATGGTAG CAGTGTGCTT 
CCACCACCTC CACCATTGCG GCAGCAGTAC GCTGAGAAGA GTGGAGGTGT GGAAGGTGGG 
ATGAGGAAAC GGAAGGAGAA GAGGCCTGCG GAGGTCGAAA TGTCGGCTCG CGACTCTCCA 
TCAACATCCT CAGCGTCCCA TTCTTCTGAT GACGAGTCAT CGCCAGCGCA CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494384.2
CDS	82..4158
Translation	MKVHSEQPPGKDEPGPIRRFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_061983.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061983.7

ORF Insert Sequence:

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA TGTCATCACG TGCCGAGTTG 
GCTGGCTACG AGAAGCAGGC CGACGAGTAT AAGCGTCACG AGCCGTGGTA CACCGTCGGA 
GGATTCCTCA ACAAGATCTA CATTCCGGCA CTCAAGAATA ATGTTGTTAA GGCTTGCGTT 
CTCATCGGAA CTACCACCGC CGTCGTCTTC GGACTCTACG GAATGTACAC CTCGACTCTC 
GGTCTCGAGC TCGCCGACGT GCTTCCAGAG CACACCCCAC CAGCGGCCTT CCTTCGTGCT 
CGCGAGCAAT ACTTCTCCTT CTACCCGATG TTTGCAGTTC TTCGTGGTGA CAAGCTTGAC 
ATTCCGAACC AACAGCAACT TATCGAAGAG TACAGAGCAC AGTTGGGATC TTCAAAGTTT 
ATGATCAAGG CTGAAGGTAA ACTTCAACCC TACTGGATGT CTATGCTCAG AGTCTGGCTG 
CAATCTTTGG ACATGGCCTT GGAAAAAGAC CTCGCCGCTG GCAAATTCGA TTTGACAAAT 
GGAAATCCTA TCAAGGTGAA CGGAGAGAAG CCAAGTCCCG AATCGATGAT CGCGGCTAGA 
CTTGTATGCT CCTTCGGCAC CAACTACAAC TGTGACGGGC GGCTCGGAAA GATGAAGATG 
GTGGAAAATG AGGTTATTAA TCCAGAAGGA TTCTATAACT ATCTGACGGG ATGGTTCAAC 
GTTGACAACA TGATGTACTA CGTGTCCCAG GCTTCATTCT ATCCAACACC ACCTGGATGG 
GAGTATAATG AGAAGCTCGC CAAGGTGGTT CCGGCTGCCG AGCCATTGTT GTACTCACAG 
ATGCCGTTTT ATCAGAATGA TTTGATCGAT ACACCAGCGA TTGTAAAAAT GATTGAGGAA 
ATCCGTGCCA CTTGCGAGGA ATACTCCGAA CGAGGCCTTT CCAACCATCC ATCGGGTATC 
GCCTTCACCT TCTGGGAGCA ATATCTTACT TTGAGATGGA ACTTGTTCCA AGCCATCTGC 
ATCATCGCGC TCGCCGTGTT CTGTGTCATT TCAATTCTAA TGTTCAATCC ATGGGCAGCC 
ACTCTTATCA TGTGCATCGT AGTGATCACT ACCATCGAGC TGGGAGGATT CATGGGGCTT 
ATGGGCATCA AGATGAACCC GATTTCCGCT GTAACCCTCA TCTGTGCCGT CGGAATCGGT 
GTCGAGTTCA CTGCTCACGT CGAGTTGGCC TTCCTGACAG CTCTAGGAAC CATCGATCAA 
CGCCTGGAGT CTTGCCTCCA GCACATGTTC GTGCCAGTCT ATCACGGAGC CATCTCAACG 
TTCCTCGGAG TTGTGATGCT TGTGTTCTCC GAGTTCGACT TCGTCGTCAC CTACTTCTTC 
TACACAATGA CGTTGCTGGT AGCGTTGGGT GTGTTCAACG GGTTGTGTGT TCTTCCAGTG 
ATCCTTACGC TGGTCGGCCC GAAGCCTGAG CTGACACCAA CCGATGGTAG CAGTGTGCTT 
CCACCACCTC CACCATTGCG GCAGCAGTAC GCTGAGAAGA GTGGAGGTGT GGAAGGTGGG 
ATGAGGAAAC GGAAGGAGAA GAGGCCTGCG GAGGTCGAAA TGTCGGCTCG CGACTCTCCA 
TCAACATCCT CAGCGTCCCA TTCTTCTGAT GACGAGTCAT CGCCAGCGCA CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptc-3 ( NM_061983.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494384.2 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Price & Availability↑

CloneID	OCa339378
Clone ID Related Accession (Same CDS sequence)	NM_061983.7 , NM_061983.6
Accession Version	NM_061983.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_061983.5. COMPLETENESS: incomplete on both ends.

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA TGTCATCACG TGCCGAGTTG 
GCTGGCTACG AGAAGCAGGC CGACGAGTAT AAGCGTCACG AGCCGTGGTA CACCGTCGGA 
GGATTCCTCA ACAAGATCTA CATTCCGGCA CTCAAGAATA ATGTTGTTAA GGCTTGCGTT 
CTCATCGGAA CTACCACCGC CGTCGTCTTC GGACTCTACG GAATGTACAC CTCGACTCTC 
GGTCTCGAGC TCGCCGACGT GCTTCCAGAG CACACCCCAC CAGCGGCCTT CCTTCGTGCT 
CGCGAGCAAT ACTTCTCCTT CTACCCGATG TTTGCAGTTC TTCGTGGTGA CAAGCTTGAC 
ATTCCGAACC AACAGCAACT TATCGAAGAG TACAGAGCAC AGTTGGGATC TTCAAAGTTT 
ATGATCAAGG CTGAAGGTAA ACTTCAACCC TACTGGATGT CTATGCTCAG AGTCTGGCTG 
CAATCTTTGG ACATGGCCTT GGAAAAAGAC CTCGCCGCTG GCAAATTCGA TTTGACAAAT 
GGAAATCCTA TCAAGGTGAA CGGAGAGAAG CCAAGTCCCG AATCGATGAT CGCGGCTAGA 
CTTGTATGCT CCTTCGGCAC CAACTACAAC TGTGACGGGC GGCTCGGAAA GATGAAGATG 
GTGGAAAATG AGGTTATTAA TCCAGAAGGA TTCTATAACT ATCTGACGGG ATGGTTCAAC 
GTTGACAACA TGATGTACTA CGTGTCCCAG GCTTCATTCT ATCCAACACC ACCTGGATGG 
GAGTATAATG AGAAGCTCGC CAAGGTGGTT CCGGCTGCCG AGCCATTGTT GTACTCACAG 
ATGCCGTTTT ATCAGAATGA TTTGATCGAT ACACCAGCGA TTGTAAAAAT GATTGAGGAA 
ATCCGTGCCA CTTGCGAGGA ATACTCCGAA CGAGGCCTTT CCAACCATCC ATCGGGTATC 
GCCTTCACCT TCTGGGAGCA ATATCTTACT TTGAGATGGA ACTTGTTCCA AGCCATCTGC 
ATCATCGCGC TCGCCGTGTT CTGTGTCATT TCAATTCTAA TGTTCAATCC ATGGGCAGCC 
ACTCTTATCA TGTGCATCGT AGTGATCACT ACCATCGAGC TGGGAGGATT CATGGGGCTT 
ATGGGCATCA AGATGAACCC GATTTCCGCT GTAACCCTCA TCTGTGCCGT CGGAATCGGT 
GTCGAGTTCA CTGCTCACGT CGAGTTGGCC TTCCTGACAG CTCTAGGAAC CATCGATCAA 
CGCCTGGAGT CTTGCCTCCA GCACATGTTC GTGCCAGTCT ATCACGGAGC CATCTCAACG 
TTCCTCGGAG TTGTGATGCT TGTGTTCTCC GAGTTCGACT TCGTCGTCAC CTACTTCTTC 
TACACAATGA CGTTGCTGGT AGCGTTGGGT GTGTTCAACG GGTTGTGTGT TCTTCCAGTG 
ATCCTTACGC TGGTCGGCCC GAAGCCTGAG CTGACACCAA CCGATGGTAG CAGTGTGCTT 
CCACCACCTC CACCATTGCG GCAGCAGTAC GCTGAGAAGA GTGGAGGTGT GGAAGGTGGG 
ATGAGGAAAC GGAAGGAGAA GAGGCCTGCG GAGGTCGAAA TGTCGGCTCG CGACTCTCCA 
TCAACATCCT CAGCGTCCCA TTCTTCTGAT GACGAGTCAT CGCCAGCGCA CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494384.2
CDS	1..4077
Translation	MKVHSEQPPGKDEPGPIRRFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_061983.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061983.6

ORF Insert Sequence:

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA TGTCATCACG TGCCGAGTTG 
GCTGGCTACG AGAAGCAGGC CGACGAGTAT AAGCGTCACG AGCCGTGGTA CACCGTCGGA 
GGATTCCTCA ACAAGATCTA CATTCCGGCA CTCAAGAATA ATGTTGTTAA GGCTTGCGTT 
CTCATCGGAA CTACCACCGC CGTCGTCTTC GGACTCTACG GAATGTACAC CTCGACTCTC 
GGTCTCGAGC TCGCCGACGT GCTTCCAGAG CACACCCCAC CAGCGGCCTT CCTTCGTGCT 
CGCGAGCAAT ACTTCTCCTT CTACCCGATG TTTGCAGTTC TTCGTGGTGA CAAGCTTGAC 
ATTCCGAACC AACAGCAACT TATCGAAGAG TACAGAGCAC AGTTGGGATC TTCAAAGTTT 
ATGATCAAGG CTGAAGGTAA ACTTCAACCC TACTGGATGT CTATGCTCAG AGTCTGGCTG 
CAATCTTTGG ACATGGCCTT GGAAAAAGAC CTCGCCGCTG GCAAATTCGA TTTGACAAAT 
GGAAATCCTA TCAAGGTGAA CGGAGAGAAG CCAAGTCCCG AATCGATGAT CGCGGCTAGA 
CTTGTATGCT CCTTCGGCAC CAACTACAAC TGTGACGGGC GGCTCGGAAA GATGAAGATG 
GTGGAAAATG AGGTTATTAA TCCAGAAGGA TTCTATAACT ATCTGACGGG ATGGTTCAAC 
GTTGACAACA TGATGTACTA CGTGTCCCAG GCTTCATTCT ATCCAACACC ACCTGGATGG 
GAGTATAATG AGAAGCTCGC CAAGGTGGTT CCGGCTGCCG AGCCATTGTT GTACTCACAG 
ATGCCGTTTT ATCAGAATGA TTTGATCGAT ACACCAGCGA TTGTAAAAAT GATTGAGGAA 
ATCCGTGCCA CTTGCGAGGA ATACTCCGAA CGAGGCCTTT CCAACCATCC ATCGGGTATC 
GCCTTCACCT TCTGGGAGCA ATATCTTACT TTGAGATGGA ACTTGTTCCA AGCCATCTGC 
ATCATCGCGC TCGCCGTGTT CTGTGTCATT TCAATTCTAA TGTTCAATCC ATGGGCAGCC 
ACTCTTATCA TGTGCATCGT AGTGATCACT ACCATCGAGC TGGGAGGATT CATGGGGCTT 
ATGGGCATCA AGATGAACCC GATTTCCGCT GTAACCCTCA TCTGTGCCGT CGGAATCGGT 
GTCGAGTTCA CTGCTCACGT CGAGTTGGCC TTCCTGACAG CTCTAGGAAC CATCGATCAA 
CGCCTGGAGT CTTGCCTCCA GCACATGTTC GTGCCAGTCT ATCACGGAGC CATCTCAACG 
TTCCTCGGAG TTGTGATGCT TGTGTTCTCC GAGTTCGACT TCGTCGTCAC CTACTTCTTC 
TACACAATGA CGTTGCTGGT AGCGTTGGGT GTGTTCAACG GGTTGTGTGT TCTTCCAGTG 
ATCCTTACGC TGGTCGGCCC GAAGCCTGAG CTGACACCAA CCGATGGTAG CAGTGTGCTT 
CCACCACCTC CACCATTGCG GCAGCAGTAC GCTGAGAAGA GTGGAGGTGT GGAAGGTGGG 
ATGAGGAAAC GGAAGGAGAA GAGGCCTGCG GAGGTCGAAA TGTCGGCTCG CGACTCTCCA 
TCAACATCCT CAGCGTCCCA TTCTTCTGAT GACGAGTCAT CGCCAGCGCA CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptc-3 ( NM_061982.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494383.2 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

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CloneID	OCa339379
Clone ID Related Accession (Same CDS sequence)	NM_061982.5 , NM_061982.6
Accession Version	NM_061982.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_061982.4. COMPLETENESS: incomplete on both ends.

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA ACACGCAGAT GTCATCACGT 
GCCGAGTTGG CTGGCTACGA GAAGCAGGCC GACGAGTATA AGCGTCACGA GCCGTGGTAC 
ACCGTCGGAG GATTCCTCAA CAAGATCTAC ATTCCGGCAC TCAAGAATAA TGTTGTTAAG 
GCTTGCGTTC TCATCGGAAC TACCACCGCC GTCGTCTTCG GACTCTACGG AATGTACACC 
TCGACTCTCG GTCTCGAGCT CGCCGACGTG CTTCCAGAGC ACACCCCACC AGCGGCCTTC 
CTTCGTGCTC GCGAGCAATA CTTCTCCTTC TACCCGATGT TTGCAGTTCT TCGTGGTGAC 
AAGCTTGACA TTCCGAACCA ACAGCAACTT ATCGAAGAGT ACAGAGCACA GTTGGGATCT 
TCAAAGTTTA TGATCAAGGC TGAAGGTAAA CTTCAACCCT ACTGGATGTC TATGCTCAGA 
GTCTGGCTGC AATCTTTGGA CATGGCCTTG GAAAAAGACC TCGCCGCTGG CAAATTCGAT 
TTGACAAATG GAAATCCTAT CAAGGTGAAC GGAGAGAAGC CAAGTCCCGA ATCGATGATC 
GCGGCTAGAC TTGTATGCTC CTTCGGCACC AACTACAACT GTGACGGGCG GCTCGGAAAG 
ATGAAGATGG TGGAAAATGA GGTTATTAAT CCAGAAGGAT TCTATAACTA TCTGACGGGA 
TGGTTCAACG TTGACAACAT GATGTACTAC GTGTCCCAGG CTTCATTCTA TCCAACACCA 
CCTGGATGGG AGTATAATGA GAAGCTCGCC AAGGTGGTTC CGGCTGCCGA GCCATTGTTG 
TACTCACAGA TGCCGTTTTA TCAGAATGAT TTGATCGATA CACCAGCGAT TGTAAAAATG 
ATTGAGGAAA TCCGTGCCAC TTGCGAGGAA TACTCCGAAC GAGGCCTTTC CAACCATCCA 
TCGGGTATCG CCTTCACCTT CTGGGAGCAA TATCTTACTT TGAGATGGAA CTTGTTCCAA 
GCCATCTGCA TCATCGCGCT CGCCGTGTTC TGTGTCATTT CAATTCTAAT GTTCAATCCA 
TGGGCAGCCA CTCTTATCAT GTGCATCGTA GTGATCACTA CCATCGAGCT GGGAGGATTC 
ATGGGGCTTA TGGGCATCAA GATGAACCCG ATTTCCGCTG TAACCCTCAT CTGTGCCGTC 
GGAATCGGTG TCGAGTTCAC TGCTCACGTC GAGTTGGCCT TCCTGACAGC TCTAGGAACC 
ATCGATCAAC GCCTGGAGTC TTGCCTCCAG CACATGTTCG TGCCAGTCTA TCACGGAGCC 
ATCTCAACGT TCCTCGGAGT TGTGATGCTT GTGTTCTCCG AGTTCGACTT CGTCGTCACC 
TACTTCTTCT ACACAATGAC GTTGCTGGTA GCGTTGGGTG TGTTCAACGG GTTGTGTGTT 
CTTCCAGTGA TCCTTACGCT GGTCGGCCCG AAGCCTGAGC TGACACCAAC CGATGGTAGC 
AGTGTGCTTC CACCACCTCC ACCATTGCGG CAGCAGTACG CTGAGAAGAG TGGAGGTGTG 
GAAGGTGGGA TGAGGAAACG GAAGGAGAAG AGGCCTGCGG AGGTCGAAAT GTCGGCTCGC 
GACTCTCCAT CAACATCCTC AGCGTCCCAT TCTTCTGATG ACGAGTCATC GCCAGCGCAC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494383.2
CDS	1..4086
Translation	MKVHSEQPPGKDEPGPIRRFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNNTQMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_061982.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061982.5

ORF Insert Sequence:

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA ACACGCAGAT GTCATCACGT 
GCCGAGTTGG CTGGCTACGA GAAGCAGGCC GACGAGTATA AGCGTCACGA GCCGTGGTAC 
ACCGTCGGAG GATTCCTCAA CAAGATCTAC ATTCCGGCAC TCAAGAATAA TGTTGTTAAG 
GCTTGCGTTC TCATCGGAAC TACCACCGCC GTCGTCTTCG GACTCTACGG AATGTACACC 
TCGACTCTCG GTCTCGAGCT CGCCGACGTG CTTCCAGAGC ACACCCCACC AGCGGCCTTC 
CTTCGTGCTC GCGAGCAATA CTTCTCCTTC TACCCGATGT TTGCAGTTCT TCGTGGTGAC 
AAGCTTGACA TTCCGAACCA ACAGCAACTT ATCGAAGAGT ACAGAGCACA GTTGGGATCT 
TCAAAGTTTA TGATCAAGGC TGAAGGTAAA CTTCAACCCT ACTGGATGTC TATGCTCAGA 
GTCTGGCTGC AATCTTTGGA CATGGCCTTG GAAAAAGACC TCGCCGCTGG CAAATTCGAT 
TTGACAAATG GAAATCCTAT CAAGGTGAAC GGAGAGAAGC CAAGTCCCGA ATCGATGATC 
GCGGCTAGAC TTGTATGCTC CTTCGGCACC AACTACAACT GTGACGGGCG GCTCGGAAAG 
ATGAAGATGG TGGAAAATGA GGTTATTAAT CCAGAAGGAT TCTATAACTA TCTGACGGGA 
TGGTTCAACG TTGACAACAT GATGTACTAC GTGTCCCAGG CTTCATTCTA TCCAACACCA 
CCTGGATGGG AGTATAATGA GAAGCTCGCC AAGGTGGTTC CGGCTGCCGA GCCATTGTTG 
TACTCACAGA TGCCGTTTTA TCAGAATGAT TTGATCGATA CACCAGCGAT TGTAAAAATG 
ATTGAGGAAA TCCGTGCCAC TTGCGAGGAA TACTCCGAAC GAGGCCTTTC CAACCATCCA 
TCGGGTATCG CCTTCACCTT CTGGGAGCAA TATCTTACTT TGAGATGGAA CTTGTTCCAA 
GCCATCTGCA TCATCGCGCT CGCCGTGTTC TGTGTCATTT CAATTCTAAT GTTCAATCCA 
TGGGCAGCCA CTCTTATCAT GTGCATCGTA GTGATCACTA CCATCGAGCT GGGAGGATTC 
ATGGGGCTTA TGGGCATCAA GATGAACCCG ATTTCCGCTG TAACCCTCAT CTGTGCCGTC 
GGAATCGGTG TCGAGTTCAC TGCTCACGTC GAGTTGGCCT TCCTGACAGC TCTAGGAACC 
ATCGATCAAC GCCTGGAGTC TTGCCTCCAG CACATGTTCG TGCCAGTCTA TCACGGAGCC 
ATCTCAACGT TCCTCGGAGT TGTGATGCTT GTGTTCTCCG AGTTCGACTT CGTCGTCACC 
TACTTCTTCT ACACAATGAC GTTGCTGGTA GCGTTGGGTG TGTTCAACGG GTTGTGTGTT 
CTTCCAGTGA TCCTTACGCT GGTCGGCCCG AAGCCTGAGC TGACACCAAC CGATGGTAGC 
AGTGTGCTTC CACCACCTCC ACCATTGCGG CAGCAGTACG CTGAGAAGAG TGGAGGTGTG 
GAAGGTGGGA TGAGGAAACG GAAGGAGAAG AGGCCTGCGG AGGTCGAAAT GTCGGCTCGC 
GACTCTCCAT CAACATCCTC AGCGTCCCAT TCTTCTGATG ACGAGTCATC GCCAGCGCAC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptc-3 ( NM_061982.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494383.2 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.

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CloneID	OCa339379
Clone ID Related Accession (Same CDS sequence)	NM_061982.5 , NM_061982.6
Accession Version	NM_061982.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_061982.5.

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA ACACGCAGAT GTCATCACGT 
GCCGAGTTGG CTGGCTACGA GAAGCAGGCC GACGAGTATA AGCGTCACGA GCCGTGGTAC 
ACCGTCGGAG GATTCCTCAA CAAGATCTAC ATTCCGGCAC TCAAGAATAA TGTTGTTAAG 
GCTTGCGTTC TCATCGGAAC TACCACCGCC GTCGTCTTCG GACTCTACGG AATGTACACC 
TCGACTCTCG GTCTCGAGCT CGCCGACGTG CTTCCAGAGC ACACCCCACC AGCGGCCTTC 
CTTCGTGCTC GCGAGCAATA CTTCTCCTTC TACCCGATGT TTGCAGTTCT TCGTGGTGAC 
AAGCTTGACA TTCCGAACCA ACAGCAACTT ATCGAAGAGT ACAGAGCACA GTTGGGATCT 
TCAAAGTTTA TGATCAAGGC TGAAGGTAAA CTTCAACCCT ACTGGATGTC TATGCTCAGA 
GTCTGGCTGC AATCTTTGGA CATGGCCTTG GAAAAAGACC TCGCCGCTGG CAAATTCGAT 
TTGACAAATG GAAATCCTAT CAAGGTGAAC GGAGAGAAGC CAAGTCCCGA ATCGATGATC 
GCGGCTAGAC TTGTATGCTC CTTCGGCACC AACTACAACT GTGACGGGCG GCTCGGAAAG 
ATGAAGATGG TGGAAAATGA GGTTATTAAT CCAGAAGGAT TCTATAACTA TCTGACGGGA 
TGGTTCAACG TTGACAACAT GATGTACTAC GTGTCCCAGG CTTCATTCTA TCCAACACCA 
CCTGGATGGG AGTATAATGA GAAGCTCGCC AAGGTGGTTC CGGCTGCCGA GCCATTGTTG 
TACTCACAGA TGCCGTTTTA TCAGAATGAT TTGATCGATA CACCAGCGAT TGTAAAAATG 
ATTGAGGAAA TCCGTGCCAC TTGCGAGGAA TACTCCGAAC GAGGCCTTTC CAACCATCCA 
TCGGGTATCG CCTTCACCTT CTGGGAGCAA TATCTTACTT TGAGATGGAA CTTGTTCCAA 
GCCATCTGCA TCATCGCGCT CGCCGTGTTC TGTGTCATTT CAATTCTAAT GTTCAATCCA 
TGGGCAGCCA CTCTTATCAT GTGCATCGTA GTGATCACTA CCATCGAGCT GGGAGGATTC 
ATGGGGCTTA TGGGCATCAA GATGAACCCG ATTTCCGCTG TAACCCTCAT CTGTGCCGTC 
GGAATCGGTG TCGAGTTCAC TGCTCACGTC GAGTTGGCCT TCCTGACAGC TCTAGGAACC 
ATCGATCAAC GCCTGGAGTC TTGCCTCCAG CACATGTTCG TGCCAGTCTA TCACGGAGCC 
ATCTCAACGT TCCTCGGAGT TGTGATGCTT GTGTTCTCCG AGTTCGACTT CGTCGTCACC 
TACTTCTTCT ACACAATGAC GTTGCTGGTA GCGTTGGGTG TGTTCAACGG GTTGTGTGTT 
CTTCCAGTGA TCCTTACGCT GGTCGGCCCG AAGCCTGAGC TGACACCAAC CGATGGTAGC 
AGTGTGCTTC CACCACCTCC ACCATTGCGG CAGCAGTACG CTGAGAAGAG TGGAGGTGTG 
GAAGGTGGGA TGAGGAAACG GAAGGAGAAG AGGCCTGCGG AGGTCGAAAT GTCGGCTCGC 
GACTCTCCAT CAACATCCTC AGCGTCCCAT TCTTCTGATG ACGAGTCATC GCCAGCGCAC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494383.2
CDS	82..4167
Translation	MKVHSEQPPGKDEPGPIRRFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNNTQMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_061982.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061982.6

ORF Insert Sequence:

ATGAAGGTGC ATTCGGAACA ACCGCCCGGG AAGGACGAAC CCGGTCCGAT CAGAAGGTTT 
CTGGAAAACC GCCTGATCGG AAATTTCGAT TCCGAAGATT TCTCAGATGC GTGGAAGAAA 
AAATTCGCCC ACGCGCCGAC TTGGTGTGAT GCAGACATGT CTCTTCAGCA GATTAAGCGG 
GGTAAAGCCG TTGGAAACAC CGTTGCGCTC ACCGCCAGAG CATTTTTCCA ATTATGGTTG 
TTCAGGATAG GATGTTTTGT GCAACGATGG GCTTGGTCCA CTATTTTTAT CAGTTTATTC 
CTCTACTGCC TCTGCCTCGG AGGCCTTCGC CACGTCACAA TCGAAACCGA TCTCGTGAAA 
CTTTGGGTGT CAGAAGGTGG ACGGCTGAAT GAAGAAATGG GATATCTCGG CCAAGTTAAA 
TTTGAACGAG AATCCGGACA CCTAGTGCAT AAAGTGAAAA GAGCCGTCGA ACAGACTACG 
GAGCCGCCGG CACCGGCAGT GAAGGCAGAA GTCCGGCGTG GTCCCGAGCT GCCAAAGGAA 
AACGGATTGG GAGGAGGTTT TCAGGTGGTA ATCCAAACTC CGTCCTTCGA TGGTCAGAAT 
ATTCTCTCCA AGGAGGCTCT GCAGCAGCAT ACCAAGTTGA TGGAGGAAAT TTCTACGTAT 
GAAGTGAAAA TGTTCAATGA AACCTGGACC CTCTCCGACA TCTGCTTCAA GCCGCCAGGC 
CCATCTTTCA ACAGTGGCCC GCTGGCTGGA ATCATGTCCA AGCTCCTTGA CAAGATCATT 
CCATGCATCT GGATCACACC GATCGATTGC TATTGGGATG GTGCAAAGCC ACTGGGACCA 
AATCCTCCGT TGAACTTGGG CCCAGAAGTC GCCAGCTTCG TGTCCTCCCT CCCACCTGGC 
AACGTTACCT GGAAGAACTT GAATCCAACA TCTGTCATCA AAGAGGTCGG CACCCTATTC 
GACTTGGGAC CCATCGGCAA CTTTTTCGAA AGAGCCGGTA TTGACGGAGC CTACCTCGAC 
AGGCCATGCA TTGACCCACT GGAGGAGGAA TGCCCAAAGT CTGCTCCGAA CTACTTCGAT 
AGGTGTCATG CACTGACAAA GTTTAACGAG TGGAATATGG CAAAGGCCAT GAGCGAGCAG 
GTCACCCTGG AGCGCAAAAT AATCCCAAAG GACGATGGAA AGTCAGACAT CGCCGAAACG 
ATTCTCAACG ACATTTTTGG AAAGAAGAGA AGGAAGCGAC AAGCGGATAC TACGACCAAG 
AAGCCTGCCA CGAAAAAAGA CGAAGACTAC TACGAGTACG AAGACGACGC GGACTACCTA 
GCAGGAATCG CCAACTCCAC AATCGCCAAG AAGAATCCGG AAAAGGAAGC CAAGGACCTT 
CTGTGCCTTG AGTACGGAAG CTCACTTCTC AAGTGGATGC AGGAGAATCC CGAGAGACTC 
GGCGAGTTCT TGACCAAGGA GGAGATGCCA GATTACCCGA ACTATGGAGA TGTGATGACC 
GGTGGATGTA AGGGATTCGG AAAGAAGATC ATGGAGTGGC CGGAGGACTT GATCATTGGA 
GGAATCCAGC GGGACAATGG GAAGTTGGTG TCAGCAGAGG CCCTGCAATC CGTGTTCCTG 
GTCTCAGGAG CCTATGACGT GTTTGCCAGA ATCAAGAACG ACAAAACCGA CAGCCACCCC 
GGCCTTGACC GCCACCACTT CCAACCATGG ATGGCTGGAG AGATCATCAG CACGTGGCAG 
AGAAACTTCA CCAAGCGACT CTACAGCCAT GAGCTCAACC GTGAACGCCG TCAGTTCCAC 
CCCCTAGCTT CCACCTCAAT CGCTGACATG CTCGAGGAGT TCTCCCAGTT CAACTACATC 
ATCATCGTCA TCGGATACAT CTTGATGGTG ATCTACGCCG CGTTTACCCA GGGAAGGTTC 
CAAGGATGGT GGCTGGCTGT GCAGTCAAAT GTGGCATTGG CAATTTGCGG AGTTATCCTG 
GTTACTATAT CATCGATTTG TGGATTAGGA TTCGCTACTC ATCTTGGGAT CAATTTCAAT 
GCAGCGACAA CTCAAGTTGT GCCATTCCTT TCCTTAGGTC TAGGAATCGA TGATATGTTC 
CTCCTTCTGC ATAACTACGA TGAAATCATC AATATTTGCA ACAAAAACGA GATCGGAGTA 
TTGCTAAAAG AGACCGGAAT GTCTGTGATG TTGACCTCTA TCAACAACAT TCTCGCCTTC 
ATCTCGGGAT ACGTTCTCCC GATCCCTGCT CTACGATCCT TCTGCTCCCA AACCGCGATT 
CTCCTTGCCT TCAACTTGAT CTTCCTCATG TTCATCTTCC CAGCAATGAT CGGAATCGAT 
CTGAGAAGAC AGCGAAAAGG AAAAAGAGAT TTGGCCTACT GCAGTCGAGG AAACCCACAA 
ATGGCGACTA GTCAAAGTGT GCCGAGCAAT GTTTCAAATA ACACGCAGAT GTCATCACGT 
GCCGAGTTGG CTGGCTACGA GAAGCAGGCC GACGAGTATA AGCGTCACGA GCCGTGGTAC 
ACCGTCGGAG GATTCCTCAA CAAGATCTAC ATTCCGGCAC TCAAGAATAA TGTTGTTAAG 
GCTTGCGTTC TCATCGGAAC TACCACCGCC GTCGTCTTCG GACTCTACGG AATGTACACC 
TCGACTCTCG GTCTCGAGCT CGCCGACGTG CTTCCAGAGC ACACCCCACC AGCGGCCTTC 
CTTCGTGCTC GCGAGCAATA CTTCTCCTTC TACCCGATGT TTGCAGTTCT TCGTGGTGAC 
AAGCTTGACA TTCCGAACCA ACAGCAACTT ATCGAAGAGT ACAGAGCACA GTTGGGATCT 
TCAAAGTTTA TGATCAAGGC TGAAGGTAAA CTTCAACCCT ACTGGATGTC TATGCTCAGA 
GTCTGGCTGC AATCTTTGGA CATGGCCTTG GAAAAAGACC TCGCCGCTGG CAAATTCGAT 
TTGACAAATG GAAATCCTAT CAAGGTGAAC GGAGAGAAGC CAAGTCCCGA ATCGATGATC 
GCGGCTAGAC TTGTATGCTC CTTCGGCACC AACTACAACT GTGACGGGCG GCTCGGAAAG 
ATGAAGATGG TGGAAAATGA GGTTATTAAT CCAGAAGGAT TCTATAACTA TCTGACGGGA 
TGGTTCAACG TTGACAACAT GATGTACTAC GTGTCCCAGG CTTCATTCTA TCCAACACCA 
CCTGGATGGG AGTATAATGA GAAGCTCGCC AAGGTGGTTC CGGCTGCCGA GCCATTGTTG 
TACTCACAGA TGCCGTTTTA TCAGAATGAT TTGATCGATA CACCAGCGAT TGTAAAAATG 
ATTGAGGAAA TCCGTGCCAC TTGCGAGGAA TACTCCGAAC GAGGCCTTTC CAACCATCCA 
TCGGGTATCG CCTTCACCTT CTGGGAGCAA TATCTTACTT TGAGATGGAA CTTGTTCCAA 
GCCATCTGCA TCATCGCGCT CGCCGTGTTC TGTGTCATTT CAATTCTAAT GTTCAATCCA 
TGGGCAGCCA CTCTTATCAT GTGCATCGTA GTGATCACTA CCATCGAGCT GGGAGGATTC 
ATGGGGCTTA TGGGCATCAA GATGAACCCG ATTTCCGCTG TAACCCTCAT CTGTGCCGTC 
GGAATCGGTG TCGAGTTCAC TGCTCACGTC GAGTTGGCCT TCCTGACAGC TCTAGGAACC 
ATCGATCAAC GCCTGGAGTC TTGCCTCCAG CACATGTTCG TGCCAGTCTA TCACGGAGCC 
ATCTCAACGT TCCTCGGAGT TGTGATGCTT GTGTTCTCCG AGTTCGACTT CGTCGTCACC 
TACTTCTTCT ACACAATGAC GTTGCTGGTA GCGTTGGGTG TGTTCAACGG GTTGTGTGTT 
CTTCCAGTGA TCCTTACGCT GGTCGGCCCG AAGCCTGAGC TGACACCAAC CGATGGTAGC 
AGTGTGCTTC CACCACCTCC ACCATTGCGG CAGCAGTACG CTGAGAAGAG TGGAGGTGTG 
GAAGGTGGGA TGAGGAAACG GAAGGAGAAG AGGCCTGCGG AGGTCGAAAT GTCGGCTCGC 
GACTCTCCAT CAACATCCTC AGCGTCCCAT TCTTCTGATG ACGAGTCATC GCCAGCGCAC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptc-3 ( NM_001129178.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122650.1 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Price & Availability↑

CloneID	OCa339381
Clone ID Related Accession (Same CDS sequence)	NM_001129178.2 , NM_001129178.1
Accession Version	NM_001129178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001129178.1. COMPLETENESS: incomplete on the 5' end.

ATGTCGTTTC CTGATGAGGA AACTGATTTA TTATCAGAAG CTAAACATTG TATTCGGAAT 
GTTATTCATC GTACTCATAG GAAGTTTCTG GAAAACCGCC TGATCGGAAA TTTCGATTCC 
GAAGATTTCT CAGATGCGTG GAAGAAAAAA TTCGCCCACG CGCCGACTTG GTGTGATGCA 
GACATGTCTC TTCAGCAGAT TAAGCGGGGT AAAGCCGTTG GAAACACCGT TGCGCTCACC 
GCCAGAGCAT TTTTCCAATT ATGGTTGTTC AGGATAGGAT GTTTTGTGCA ACGATGGGCT 
TGGTCCACTA TTTTTATCAG TTTATTCCTC TACTGCCTCT GCCTCGGAGG CCTTCGCCAC 
GTCACAATCG AAACCGATCT CGTGAAACTT TGGGTGTCAG AAGGTGGACG GCTGAATGAA 
GAAATGGGAT ATCTCGGCCA AGTTAAATTT GAACGAGAAT CCGGACACCT AGTGCATAAA 
GTGAAAAGAG CCGTCGAACA GACTACGGAG CCGCCGGCAC CGGCAGTGAA GGCAGAAGTC 
CGGCGTGGTC CCGAGCTGCC AAAGGAAAAC GGATTGGGAG GAGGTTTTCA GGTGGTAATC 
CAAACTCCGT CCTTCGATGG TCAGAATATT CTCTCCAAGG AGGCTCTGCA GCAGCATACC 
AAGTTGATGG AGGAAATTTC TACGTATGAA GTGAAAATGT TCAATGAAAC CTGGACCCTC 
TCCGACATCT GCTTCAAGCC GCCAGGCCCA TCTTTCAACA GTGGCCCGCT GGCTGGAATC 
ATGTCCAAGC TCCTTGACAA GATCATTCCA TGCATCTGGA TCACACCGAT CGATTGCTAT 
TGGGATGGTG CAAAGCCACT GGGACCAAAT CCTCCGTTGA ACTTGGGCCC AGAAGTCGCC 
AGCTTCGTGT CCTCCCTCCC ACCTGGCAAC GTTACCTGGA AGAACTTGAA TCCAACATCT 
GTCATCAAAG AGGTCGGCAC CCTATTCGAC TTGGGACCCA TCGGCAACTT TTTCGAAAGA 
GCCGGTATTG ACGGAGCCTA CCTCGACAGG CCATGCATTG ACCCACTGGA GGAGGAATGC 
CCAAAGTCTG CTCCGAACTA CTTCGATAGG TGTCATGCAC TGACAAAGTT TAACGAGTGG 
AATATGGCAA AGGCCATGAG CGAGCAGGTC ACCCTGGAGC GCAAAATAAT CCCAAAGGAC 
GATGGAAAGT CAGACATCGC CGAAACGATT CTCAACGACA TTTTTGGAAA GAAGAGAAGG 
AAGCGACAAG CGGATACTAC GACCAAGAAG CCTGCCACGA AAAAAGACGA AGACTACTAC 
GAGTACGAAG ACGACGCGGA CTACCTAGCA GGAATCGCCA ACTCCACAAT CGCCAAGAAG 
AATCCGGAAA AGGAAGCCAA GGACCTTCTG TGCCTTGAGT ACGGAAGCTC ACTTCTCAAG 
TGGATGCAGG AGAATCCCGA GAGACTCGGC GAGTTCTTGA CCAAGGAGGA GATGCCAGAT 
TACCCGAACT ATGGAGATGT GATGACCGGT GGATGTAAGG GATTCGGAAA GAAGATCATG 
GAGTGGCCGG AGGACTTGAT CATTGGAGGA ATCCAGCGGG ACAATGGGAA GTTGGTGTCA 
GCAGAGGCCC TGCAATCCGT GTTCCTGGTC TCAGGAGCCT ATGACGTGTT TGCCAGAATC 
AAGAACGACA AAACCGACAG CCACCCCGGC CTTGACCGCC ACCACTTCCA ACCATGGATG 
GCTGGAGAGA TCATCAGCAC GTGGCAGAGA AACTTCACCA AGCGACTCTA CAGCCATGAG 
CTCAACCGTG AACGCCGTCA GTTCCACCCC CTAGCTTCCA CCTCAATCGC TGACATGCTC 
GAGGAGTTCT CCCAGTTCAA CTACATCATC ATCGTCATCG GATACATCTT GATGGTGATC 
TACGCCGCGT TTACCCAGGG AAGGTTCCAA GGATGGTGGC TGGCTGTGCA GTCAAATGTG 
GCATTGGCAA TTTGCGGAGT TATCCTGGTT ACTATATCAT CGATTTGTGG ATTAGGATTC 
GCTACTCATC TTGGGATCAA TTTCAATGCA GCGACAACTC AAGTTGTGCC ATTCCTTTCC 
TTAGGTCTAG GAATCGATGA TATGTTCCTC CTTCTGCATA ACTACGATGA AATCATCAAT 
ATTTGCAACA AAAACGAGAT CGGAGTATTG CTAAAAGAGA CCGGAATGTC TGTGATGTTG 
ACCTCTATCA ACAACATTCT CGCCTTCATC TCGGGATACG TTCTCCCGAT CCCTGCTCTA 
CGATCCTTCT GCTCCCAAAC CGCGATTCTC CTTGCCTTCA ACTTGATCTT CCTCATGTTC 
ATCTTCCCAG CAATGATCGG AATCGATCTG AGAAGACAGC GAAAAGGAAA AAGAGATTTG 
GCCTACTGCA GTCGAGGAAA CCCACAAATG GCGACTAGTC AAAGTGTGCC GAGCAATGTT 
TCAAATATGT CATCACGTGC CGAGTTGGCT GGCTACGAGA AGCAGGCCGA CGAGTATAAG 
CGTCACGAGC CGTGGTACAC CGTCGGAGGA TTCCTCAACA AGATCTACAT TCCGGCACTC 
AAGAATAATG TTGTTAAGGC TTGCGTTCTC ATCGGAACTA CCACCGCCGT CGTCTTCGGA 
CTCTACGGAA TGTACACCTC GACTCTCGGT CTCGAGCTCG CCGACGTGCT TCCAGAGCAC 
ACCCCACCAG CGGCCTTCCT TCGTGCTCGC GAGCAATACT TCTCCTTCTA CCCGATGTTT 
GCAGTTCTTC GTGGTGACAA GCTTGACATT CCGAACCAAC AGCAACTTAT CGAAGAGTAC 
AGAGCACAGT TGGGATCTTC AAAGTTTATG ATCAAGGCTG AAGGTAAACT TCAACCCTAC 
TGGATGTCTA TGCTCAGAGT CTGGCTGCAA TCTTTGGACA TGGCCTTGGA AAAAGACCTC 
GCCGCTGGCA AATTCGATTT GACAAATGGA AATCCTATCA AGGTGAACGG AGAGAAGCCA 
AGTCCCGAAT CGATGATCGC GGCTAGACTT GTATGCTCCT TCGGCACCAA CTACAACTGT 
GACGGGCGGC TCGGAAAGAT GAAGATGGTG GAAAATGAGG TTATTAATCC AGAAGGATTC 
TATAACTATC TGACGGGATG GTTCAACGTT GACAACATGA TGTACTACGT GTCCCAGGCT 
TCATTCTATC CAACACCACC TGGATGGGAG TATAATGAGA AGCTCGCCAA GGTGGTTCCG 
GCTGCCGAGC CATTGTTGTA CTCACAGATG CCGTTTTATC AGAATGATTT GATCGATACA 
CCAGCGATTG TAAAAATGAT TGAGGAAATC CGTGCCACTT GCGAGGAATA CTCCGAACGA 
GGCCTTTCCA ACCATCCATC GGGTATCGCC TTCACCTTCT GGGAGCAATA TCTTACTTTG 
AGATGGAACT TGTTCCAAGC CATCTGCATC ATCGCGCTCG CCGTGTTCTG TGTCATTTCA 
ATTCTAATGT TCAATCCATG GGCAGCCACT CTTATCATGT GCATCGTAGT GATCACTACC 
ATCGAGCTGG GAGGATTCAT GGGGCTTATG GGCATCAAGA TGAACCCGAT TTCCGCTGTA 
ACCCTCATCT GTGCCGTCGG AATCGGTGTC GAGTTCACTG CTCACGTCGA GTTGGCCTTC 
CTGACAGCTC TAGGAACCAT CGATCAACGC CTGGAGTCTT GCCTCCAGCA CATGTTCGTG 
CCAGTCTATC ACGGAGCCAT CTCAACGTTC CTCGGAGTTG TGATGCTTGT GTTCTCCGAG 
TTCGACTTCG TCGTCACCTA CTTCTTCTAC ACAATGACGT TGCTGGTAGC GTTGGGTGTG 
TTCAACGGGT TGTGTGTTCT TCCAGTGATC CTTACGCTGG TCGGCCCGAA GCCTGAGCTG 
ACACCAACCG ATGGTAGCAG TGTGCTTCCA CCACCTCCAC CATTGCGGCA GCAGTACGCT 
GAGAAGAGTG GAGGTGTGGA AGGTGGGATG AGGAAACGGA AGGAGAAGAG GCCTGCGGAG 
GTCGAAATGT CGGCTCGCGA CTCTCCATCA ACATCCTCAG CGTCCCATTC TTCTGATGAC 
GAGTCATCGC CAGCGCACAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122650.1
CDS	1..4104
Translation	MSFPDEETDLLSEAKHCIRNVIHRTHRKFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_001129178.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129178.2

ORF Insert Sequence:

ATGTCGTTTC CTGATGAGGA AACTGATTTA TTATCAGAAG CTAAACATTG TATTCGGAAT 
GTTATTCATC GTACTCATAG GAAGTTTCTG GAAAACCGCC TGATCGGAAA TTTCGATTCC 
GAAGATTTCT CAGATGCGTG GAAGAAAAAA TTCGCCCACG CGCCGACTTG GTGTGATGCA 
GACATGTCTC TTCAGCAGAT TAAGCGGGGT AAAGCCGTTG GAAACACCGT TGCGCTCACC 
GCCAGAGCAT TTTTCCAATT ATGGTTGTTC AGGATAGGAT GTTTTGTGCA ACGATGGGCT 
TGGTCCACTA TTTTTATCAG TTTATTCCTC TACTGCCTCT GCCTCGGAGG CCTTCGCCAC 
GTCACAATCG AAACCGATCT CGTGAAACTT TGGGTGTCAG AAGGTGGACG GCTGAATGAA 
GAAATGGGAT ATCTCGGCCA AGTTAAATTT GAACGAGAAT CCGGACACCT AGTGCATAAA 
GTGAAAAGAG CCGTCGAACA GACTACGGAG CCGCCGGCAC CGGCAGTGAA GGCAGAAGTC 
CGGCGTGGTC CCGAGCTGCC AAAGGAAAAC GGATTGGGAG GAGGTTTTCA GGTGGTAATC 
CAAACTCCGT CCTTCGATGG TCAGAATATT CTCTCCAAGG AGGCTCTGCA GCAGCATACC 
AAGTTGATGG AGGAAATTTC TACGTATGAA GTGAAAATGT TCAATGAAAC CTGGACCCTC 
TCCGACATCT GCTTCAAGCC GCCAGGCCCA TCTTTCAACA GTGGCCCGCT GGCTGGAATC 
ATGTCCAAGC TCCTTGACAA GATCATTCCA TGCATCTGGA TCACACCGAT CGATTGCTAT 
TGGGATGGTG CAAAGCCACT GGGACCAAAT CCTCCGTTGA ACTTGGGCCC AGAAGTCGCC 
AGCTTCGTGT CCTCCCTCCC ACCTGGCAAC GTTACCTGGA AGAACTTGAA TCCAACATCT 
GTCATCAAAG AGGTCGGCAC CCTATTCGAC TTGGGACCCA TCGGCAACTT TTTCGAAAGA 
GCCGGTATTG ACGGAGCCTA CCTCGACAGG CCATGCATTG ACCCACTGGA GGAGGAATGC 
CCAAAGTCTG CTCCGAACTA CTTCGATAGG TGTCATGCAC TGACAAAGTT TAACGAGTGG 
AATATGGCAA AGGCCATGAG CGAGCAGGTC ACCCTGGAGC GCAAAATAAT CCCAAAGGAC 
GATGGAAAGT CAGACATCGC CGAAACGATT CTCAACGACA TTTTTGGAAA GAAGAGAAGG 
AAGCGACAAG CGGATACTAC GACCAAGAAG CCTGCCACGA AAAAAGACGA AGACTACTAC 
GAGTACGAAG ACGACGCGGA CTACCTAGCA GGAATCGCCA ACTCCACAAT CGCCAAGAAG 
AATCCGGAAA AGGAAGCCAA GGACCTTCTG TGCCTTGAGT ACGGAAGCTC ACTTCTCAAG 
TGGATGCAGG AGAATCCCGA GAGACTCGGC GAGTTCTTGA CCAAGGAGGA GATGCCAGAT 
TACCCGAACT ATGGAGATGT GATGACCGGT GGATGTAAGG GATTCGGAAA GAAGATCATG 
GAGTGGCCGG AGGACTTGAT CATTGGAGGA ATCCAGCGGG ACAATGGGAA GTTGGTGTCA 
GCAGAGGCCC TGCAATCCGT GTTCCTGGTC TCAGGAGCCT ATGACGTGTT TGCCAGAATC 
AAGAACGACA AAACCGACAG CCACCCCGGC CTTGACCGCC ACCACTTCCA ACCATGGATG 
GCTGGAGAGA TCATCAGCAC GTGGCAGAGA AACTTCACCA AGCGACTCTA CAGCCATGAG 
CTCAACCGTG AACGCCGTCA GTTCCACCCC CTAGCTTCCA CCTCAATCGC TGACATGCTC 
GAGGAGTTCT CCCAGTTCAA CTACATCATC ATCGTCATCG GATACATCTT GATGGTGATC 
TACGCCGCGT TTACCCAGGG AAGGTTCCAA GGATGGTGGC TGGCTGTGCA GTCAAATGTG 
GCATTGGCAA TTTGCGGAGT TATCCTGGTT ACTATATCAT CGATTTGTGG ATTAGGATTC 
GCTACTCATC TTGGGATCAA TTTCAATGCA GCGACAACTC AAGTTGTGCC ATTCCTTTCC 
TTAGGTCTAG GAATCGATGA TATGTTCCTC CTTCTGCATA ACTACGATGA AATCATCAAT 
ATTTGCAACA AAAACGAGAT CGGAGTATTG CTAAAAGAGA CCGGAATGTC TGTGATGTTG 
ACCTCTATCA ACAACATTCT CGCCTTCATC TCGGGATACG TTCTCCCGAT CCCTGCTCTA 
CGATCCTTCT GCTCCCAAAC CGCGATTCTC CTTGCCTTCA ACTTGATCTT CCTCATGTTC 
ATCTTCCCAG CAATGATCGG AATCGATCTG AGAAGACAGC GAAAAGGAAA AAGAGATTTG 
GCCTACTGCA GTCGAGGAAA CCCACAAATG GCGACTAGTC AAAGTGTGCC GAGCAATGTT 
TCAAATATGT CATCACGTGC CGAGTTGGCT GGCTACGAGA AGCAGGCCGA CGAGTATAAG 
CGTCACGAGC CGTGGTACAC CGTCGGAGGA TTCCTCAACA AGATCTACAT TCCGGCACTC 
AAGAATAATG TTGTTAAGGC TTGCGTTCTC ATCGGAACTA CCACCGCCGT CGTCTTCGGA 
CTCTACGGAA TGTACACCTC GACTCTCGGT CTCGAGCTCG CCGACGTGCT TCCAGAGCAC 
ACCCCACCAG CGGCCTTCCT TCGTGCTCGC GAGCAATACT TCTCCTTCTA CCCGATGTTT 
GCAGTTCTTC GTGGTGACAA GCTTGACATT CCGAACCAAC AGCAACTTAT CGAAGAGTAC 
AGAGCACAGT TGGGATCTTC AAAGTTTATG ATCAAGGCTG AAGGTAAACT TCAACCCTAC 
TGGATGTCTA TGCTCAGAGT CTGGCTGCAA TCTTTGGACA TGGCCTTGGA AAAAGACCTC 
GCCGCTGGCA AATTCGATTT GACAAATGGA AATCCTATCA AGGTGAACGG AGAGAAGCCA 
AGTCCCGAAT CGATGATCGC GGCTAGACTT GTATGCTCCT TCGGCACCAA CTACAACTGT 
GACGGGCGGC TCGGAAAGAT GAAGATGGTG GAAAATGAGG TTATTAATCC AGAAGGATTC 
TATAACTATC TGACGGGATG GTTCAACGTT GACAACATGA TGTACTACGT GTCCCAGGCT 
TCATTCTATC CAACACCACC TGGATGGGAG TATAATGAGA AGCTCGCCAA GGTGGTTCCG 
GCTGCCGAGC CATTGTTGTA CTCACAGATG CCGTTTTATC AGAATGATTT GATCGATACA 
CCAGCGATTG TAAAAATGAT TGAGGAAATC CGTGCCACTT GCGAGGAATA CTCCGAACGA 
GGCCTTTCCA ACCATCCATC GGGTATCGCC TTCACCTTCT GGGAGCAATA TCTTACTTTG 
AGATGGAACT TGTTCCAAGC CATCTGCATC ATCGCGCTCG CCGTGTTCTG TGTCATTTCA 
ATTCTAATGT TCAATCCATG GGCAGCCACT CTTATCATGT GCATCGTAGT GATCACTACC 
ATCGAGCTGG GAGGATTCAT GGGGCTTATG GGCATCAAGA TGAACCCGAT TTCCGCTGTA 
ACCCTCATCT GTGCCGTCGG AATCGGTGTC GAGTTCACTG CTCACGTCGA GTTGGCCTTC 
CTGACAGCTC TAGGAACCAT CGATCAACGC CTGGAGTCTT GCCTCCAGCA CATGTTCGTG 
CCAGTCTATC ACGGAGCCAT CTCAACGTTC CTCGGAGTTG TGATGCTTGT GTTCTCCGAG 
TTCGACTTCG TCGTCACCTA CTTCTTCTAC ACAATGACGT TGCTGGTAGC GTTGGGTGTG 
TTCAACGGGT TGTGTGTTCT TCCAGTGATC CTTACGCTGG TCGGCCCGAA GCCTGAGCTG 
ACACCAACCG ATGGTAGCAG TGTGCTTCCA CCACCTCCAC CATTGCGGCA GCAGTACGCT 
GAGAAGAGTG GAGGTGTGGA AGGTGGGATG AGGAAACGGA AGGAGAAGAG GCCTGCGGAG 
GTCGAAATGT CGGCTCGCGA CTCTCCATCA ACATCCTCAG CGTCCCATTC TTCTGATGAC 
GAGTCATCGC CAGCGCACAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptc-3 ( NM_001129178.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122650.1 Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

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CloneID	OCa339381
Clone ID Related Accession (Same CDS sequence)	NM_001129178.2 , NM_001129178.1
Accession Version	NM_001129178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein patched homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGTCGTTTC CTGATGAGGA AACTGATTTA TTATCAGAAG CTAAACATTG TATTCGGAAT 
GTTATTCATC GTACTCATAG GAAGTTTCTG GAAAACCGCC TGATCGGAAA TTTCGATTCC 
GAAGATTTCT CAGATGCGTG GAAGAAAAAA TTCGCCCACG CGCCGACTTG GTGTGATGCA 
GACATGTCTC TTCAGCAGAT TAAGCGGGGT AAAGCCGTTG GAAACACCGT TGCGCTCACC 
GCCAGAGCAT TTTTCCAATT ATGGTTGTTC AGGATAGGAT GTTTTGTGCA ACGATGGGCT 
TGGTCCACTA TTTTTATCAG TTTATTCCTC TACTGCCTCT GCCTCGGAGG CCTTCGCCAC 
GTCACAATCG AAACCGATCT CGTGAAACTT TGGGTGTCAG AAGGTGGACG GCTGAATGAA 
GAAATGGGAT ATCTCGGCCA AGTTAAATTT GAACGAGAAT CCGGACACCT AGTGCATAAA 
GTGAAAAGAG CCGTCGAACA GACTACGGAG CCGCCGGCAC CGGCAGTGAA GGCAGAAGTC 
CGGCGTGGTC CCGAGCTGCC AAAGGAAAAC GGATTGGGAG GAGGTTTTCA GGTGGTAATC 
CAAACTCCGT CCTTCGATGG TCAGAATATT CTCTCCAAGG AGGCTCTGCA GCAGCATACC 
AAGTTGATGG AGGAAATTTC TACGTATGAA GTGAAAATGT TCAATGAAAC CTGGACCCTC 
TCCGACATCT GCTTCAAGCC GCCAGGCCCA TCTTTCAACA GTGGCCCGCT GGCTGGAATC 
ATGTCCAAGC TCCTTGACAA GATCATTCCA TGCATCTGGA TCACACCGAT CGATTGCTAT 
TGGGATGGTG CAAAGCCACT GGGACCAAAT CCTCCGTTGA ACTTGGGCCC AGAAGTCGCC 
AGCTTCGTGT CCTCCCTCCC ACCTGGCAAC GTTACCTGGA AGAACTTGAA TCCAACATCT 
GTCATCAAAG AGGTCGGCAC CCTATTCGAC TTGGGACCCA TCGGCAACTT TTTCGAAAGA 
GCCGGTATTG ACGGAGCCTA CCTCGACAGG CCATGCATTG ACCCACTGGA GGAGGAATGC 
CCAAAGTCTG CTCCGAACTA CTTCGATAGG TGTCATGCAC TGACAAAGTT TAACGAGTGG 
AATATGGCAA AGGCCATGAG CGAGCAGGTC ACCCTGGAGC GCAAAATAAT CCCAAAGGAC 
GATGGAAAGT CAGACATCGC CGAAACGATT CTCAACGACA TTTTTGGAAA GAAGAGAAGG 
AAGCGACAAG CGGATACTAC GACCAAGAAG CCTGCCACGA AAAAAGACGA AGACTACTAC 
GAGTACGAAG ACGACGCGGA CTACCTAGCA GGAATCGCCA ACTCCACAAT CGCCAAGAAG 
AATCCGGAAA AGGAAGCCAA GGACCTTCTG TGCCTTGAGT ACGGAAGCTC ACTTCTCAAG 
TGGATGCAGG AGAATCCCGA GAGACTCGGC GAGTTCTTGA CCAAGGAGGA GATGCCAGAT 
TACCCGAACT ATGGAGATGT GATGACCGGT GGATGTAAGG GATTCGGAAA GAAGATCATG 
GAGTGGCCGG AGGACTTGAT CATTGGAGGA ATCCAGCGGG ACAATGGGAA GTTGGTGTCA 
GCAGAGGCCC TGCAATCCGT GTTCCTGGTC TCAGGAGCCT ATGACGTGTT TGCCAGAATC 
AAGAACGACA AAACCGACAG CCACCCCGGC CTTGACCGCC ACCACTTCCA ACCATGGATG 
GCTGGAGAGA TCATCAGCAC GTGGCAGAGA AACTTCACCA AGCGACTCTA CAGCCATGAG 
CTCAACCGTG AACGCCGTCA GTTCCACCCC CTAGCTTCCA CCTCAATCGC TGACATGCTC 
GAGGAGTTCT CCCAGTTCAA CTACATCATC ATCGTCATCG GATACATCTT GATGGTGATC 
TACGCCGCGT TTACCCAGGG AAGGTTCCAA GGATGGTGGC TGGCTGTGCA GTCAAATGTG 
GCATTGGCAA TTTGCGGAGT TATCCTGGTT ACTATATCAT CGATTTGTGG ATTAGGATTC 
GCTACTCATC TTGGGATCAA TTTCAATGCA GCGACAACTC AAGTTGTGCC ATTCCTTTCC 
TTAGGTCTAG GAATCGATGA TATGTTCCTC CTTCTGCATA ACTACGATGA AATCATCAAT 
ATTTGCAACA AAAACGAGAT CGGAGTATTG CTAAAAGAGA CCGGAATGTC TGTGATGTTG 
ACCTCTATCA ACAACATTCT CGCCTTCATC TCGGGATACG TTCTCCCGAT CCCTGCTCTA 
CGATCCTTCT GCTCCCAAAC CGCGATTCTC CTTGCCTTCA ACTTGATCTT CCTCATGTTC 
ATCTTCCCAG CAATGATCGG AATCGATCTG AGAAGACAGC GAAAAGGAAA AAGAGATTTG 
GCCTACTGCA GTCGAGGAAA CCCACAAATG GCGACTAGTC AAAGTGTGCC GAGCAATGTT 
TCAAATATGT CATCACGTGC CGAGTTGGCT GGCTACGAGA AGCAGGCCGA CGAGTATAAG 
CGTCACGAGC CGTGGTACAC CGTCGGAGGA TTCCTCAACA AGATCTACAT TCCGGCACTC 
AAGAATAATG TTGTTAAGGC TTGCGTTCTC ATCGGAACTA CCACCGCCGT CGTCTTCGGA 
CTCTACGGAA TGTACACCTC GACTCTCGGT CTCGAGCTCG CCGACGTGCT TCCAGAGCAC 
ACCCCACCAG CGGCCTTCCT TCGTGCTCGC GAGCAATACT TCTCCTTCTA CCCGATGTTT 
GCAGTTCTTC GTGGTGACAA GCTTGACATT CCGAACCAAC AGCAACTTAT CGAAGAGTAC 
AGAGCACAGT TGGGATCTTC AAAGTTTATG ATCAAGGCTG AAGGTAAACT TCAACCCTAC 
TGGATGTCTA TGCTCAGAGT CTGGCTGCAA TCTTTGGACA TGGCCTTGGA AAAAGACCTC 
GCCGCTGGCA AATTCGATTT GACAAATGGA AATCCTATCA AGGTGAACGG AGAGAAGCCA 
AGTCCCGAAT CGATGATCGC GGCTAGACTT GTATGCTCCT TCGGCACCAA CTACAACTGT 
GACGGGCGGC TCGGAAAGAT GAAGATGGTG GAAAATGAGG TTATTAATCC AGAAGGATTC 
TATAACTATC TGACGGGATG GTTCAACGTT GACAACATGA TGTACTACGT GTCCCAGGCT 
TCATTCTATC CAACACCACC TGGATGGGAG TATAATGAGA AGCTCGCCAA GGTGGTTCCG 
GCTGCCGAGC CATTGTTGTA CTCACAGATG CCGTTTTATC AGAATGATTT GATCGATACA 
CCAGCGATTG TAAAAATGAT TGAGGAAATC CGTGCCACTT GCGAGGAATA CTCCGAACGA 
GGCCTTTCCA ACCATCCATC GGGTATCGCC TTCACCTTCT GGGAGCAATA TCTTACTTTG 
AGATGGAACT TGTTCCAAGC CATCTGCATC ATCGCGCTCG CCGTGTTCTG TGTCATTTCA 
ATTCTAATGT TCAATCCATG GGCAGCCACT CTTATCATGT GCATCGTAGT GATCACTACC 
ATCGAGCTGG GAGGATTCAT GGGGCTTATG GGCATCAAGA TGAACCCGAT TTCCGCTGTA 
ACCCTCATCT GTGCCGTCGG AATCGGTGTC GAGTTCACTG CTCACGTCGA GTTGGCCTTC 
CTGACAGCTC TAGGAACCAT CGATCAACGC CTGGAGTCTT GCCTCCAGCA CATGTTCGTG 
CCAGTCTATC ACGGAGCCAT CTCAACGTTC CTCGGAGTTG TGATGCTTGT GTTCTCCGAG 
TTCGACTTCG TCGTCACCTA CTTCTTCTAC ACAATGACGT TGCTGGTAGC GTTGGGTGTG 
TTCAACGGGT TGTGTGTTCT TCCAGTGATC CTTACGCTGG TCGGCCCGAA GCCTGAGCTG 
ACACCAACCG ATGGTAGCAG TGTGCTTCCA CCACCTCCAC CATTGCGGCA GCAGTACGCT 
GAGAAGAGTG GAGGTGTGGA AGGTGGGATG AGGAAACGGA AGGAGAAGAG GCCTGCGGAG 
GTCGAAATGT CGGCTCGCGA CTCTCCATCA ACATCCTCAG CGTCCCATTC TTCTGATGAC 
GAGTCATCGC CAGCGCACAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122650.1
CDS	1..4104
Translation	MSFPDEETDLLSEAKHCIRNVIHRTHRKFLENRLIGNFDSEDFSDAWKKKFAHAPTWCDADMSLQQIKRGKAVGNTVALTARAFFQLWLFRIGCFVQRWAWSTIFISLFLYCLCLGGLRHVTIETDLVKLWVSEGGRLNEEMGYLGQVKFERESGHLVHKVKRAVEQTTEPPAPAVKAEVRRGPELPKENGLGGGFQVVIQTPSFDGQNILSKEALQQHTKLMEEISTYEVKMFNETWTLSDICFKPPGPSFNSGPLAGIMSKLLDKIIPCIWITPIDCYWDGAKPLGPNPPLNLGPEVASFVSSLPPGNVTWKNLNPTSVIKEVGTLFDLGPIGNFFERAGIDGAYLDRPCIDPLEEECPKSAPNYFDRCHALTKFNEWNMAKAMSEQVTLERKIIPKDDGKSDIAETILNDIFGKKRRKRQADTTTKKPATKKDEDYYEYEDDADYLAGIANSTIAKKNPEKEAKDLLCLEYGSSLLKWMQENPERLGEFLTKEEMPDYPNYGDVMTGGCKGFGKKIMEWPEDLIIGGIQRDNGKLVSAEALQSVFLVSGAYDVFARIKNDKTDSHPGLDRHHFQPWMAGEIISTWQRNFTKRLYSHELNRERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSRGNPQMATSQSVPSNVSNMSSRAELAGYEKQADEYKRHEPWYTVGGFLNKIYIPALKNNVVKACVLIGTTTAVVFGLYGMYTSTLGLELADVLPEHTPPAAFLRAREQYFSFYPMFAVLRGDKLDIPNQQQLIEEYRAQLGSSKFMIKAEGKLQPYWMSMLRVWLQSLDMALEKDLAAGKFDLTNGNPIKVNGEKPSPESMIAARLVCSFGTNYNCDGRLGKMKMVENEVINPEGFYNYLTGWFNVDNMMYYVSQASFYPTPPGWEYNEKLAKVVPAAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTDGSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEMSARDSPSTSSASHSSDDESSPAHK

Target ORF information:

RefSeq Version	NM_001129178.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein patched homolog 3 (ptc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129178.1

ORF Insert Sequence:

ATGTCGTTTC CTGATGAGGA AACTGATTTA TTATCAGAAG CTAAACATTG TATTCGGAAT 
GTTATTCATC GTACTCATAG GAAGTTTCTG GAAAACCGCC TGATCGGAAA TTTCGATTCC 
GAAGATTTCT CAGATGCGTG GAAGAAAAAA TTCGCCCACG CGCCGACTTG GTGTGATGCA 
GACATGTCTC TTCAGCAGAT TAAGCGGGGT AAAGCCGTTG GAAACACCGT TGCGCTCACC 
GCCAGAGCAT TTTTCCAATT ATGGTTGTTC AGGATAGGAT GTTTTGTGCA ACGATGGGCT 
TGGTCCACTA TTTTTATCAG TTTATTCCTC TACTGCCTCT GCCTCGGAGG CCTTCGCCAC 
GTCACAATCG AAACCGATCT CGTGAAACTT TGGGTGTCAG AAGGTGGACG GCTGAATGAA 
GAAATGGGAT ATCTCGGCCA AGTTAAATTT GAACGAGAAT CCGGACACCT AGTGCATAAA 
GTGAAAAGAG CCGTCGAACA GACTACGGAG CCGCCGGCAC CGGCAGTGAA GGCAGAAGTC 
CGGCGTGGTC CCGAGCTGCC AAAGGAAAAC GGATTGGGAG GAGGTTTTCA GGTGGTAATC 
CAAACTCCGT CCTTCGATGG TCAGAATATT CTCTCCAAGG AGGCTCTGCA GCAGCATACC 
AAGTTGATGG AGGAAATTTC TACGTATGAA GTGAAAATGT TCAATGAAAC CTGGACCCTC 
TCCGACATCT GCTTCAAGCC GCCAGGCCCA TCTTTCAACA GTGGCCCGCT GGCTGGAATC 
ATGTCCAAGC TCCTTGACAA GATCATTCCA TGCATCTGGA TCACACCGAT CGATTGCTAT 
TGGGATGGTG CAAAGCCACT GGGACCAAAT CCTCCGTTGA ACTTGGGCCC AGAAGTCGCC 
AGCTTCGTGT CCTCCCTCCC ACCTGGCAAC GTTACCTGGA AGAACTTGAA TCCAACATCT 
GTCATCAAAG AGGTCGGCAC CCTATTCGAC TTGGGACCCA TCGGCAACTT TTTCGAAAGA 
GCCGGTATTG ACGGAGCCTA CCTCGACAGG CCATGCATTG ACCCACTGGA GGAGGAATGC 
CCAAAGTCTG CTCCGAACTA CTTCGATAGG TGTCATGCAC TGACAAAGTT TAACGAGTGG 
AATATGGCAA AGGCCATGAG CGAGCAGGTC ACCCTGGAGC GCAAAATAAT CCCAAAGGAC 
GATGGAAAGT CAGACATCGC CGAAACGATT CTCAACGACA TTTTTGGAAA GAAGAGAAGG 
AAGCGACAAG CGGATACTAC GACCAAGAAG CCTGCCACGA AAAAAGACGA AGACTACTAC 
GAGTACGAAG ACGACGCGGA CTACCTAGCA GGAATCGCCA ACTCCACAAT CGCCAAGAAG 
AATCCGGAAA AGGAAGCCAA GGACCTTCTG TGCCTTGAGT ACGGAAGCTC ACTTCTCAAG 
TGGATGCAGG AGAATCCCGA GAGACTCGGC GAGTTCTTGA CCAAGGAGGA GATGCCAGAT 
TACCCGAACT ATGGAGATGT GATGACCGGT GGATGTAAGG GATTCGGAAA GAAGATCATG 
GAGTGGCCGG AGGACTTGAT CATTGGAGGA ATCCAGCGGG ACAATGGGAA GTTGGTGTCA 
GCAGAGGCCC TGCAATCCGT GTTCCTGGTC TCAGGAGCCT ATGACGTGTT TGCCAGAATC 
AAGAACGACA AAACCGACAG CCACCCCGGC CTTGACCGCC ACCACTTCCA ACCATGGATG 
GCTGGAGAGA TCATCAGCAC GTGGCAGAGA AACTTCACCA AGCGACTCTA CAGCCATGAG 
CTCAACCGTG AACGCCGTCA GTTCCACCCC CTAGCTTCCA CCTCAATCGC TGACATGCTC 
GAGGAGTTCT CCCAGTTCAA CTACATCATC ATCGTCATCG GATACATCTT GATGGTGATC 
TACGCCGCGT TTACCCAGGG AAGGTTCCAA GGATGGTGGC TGGCTGTGCA GTCAAATGTG 
GCATTGGCAA TTTGCGGAGT TATCCTGGTT ACTATATCAT CGATTTGTGG ATTAGGATTC 
GCTACTCATC TTGGGATCAA TTTCAATGCA GCGACAACTC AAGTTGTGCC ATTCCTTTCC 
TTAGGTCTAG GAATCGATGA TATGTTCCTC CTTCTGCATA ACTACGATGA AATCATCAAT 
ATTTGCAACA AAAACGAGAT CGGAGTATTG CTAAAAGAGA CCGGAATGTC TGTGATGTTG 
ACCTCTATCA ACAACATTCT CGCCTTCATC TCGGGATACG TTCTCCCGAT CCCTGCTCTA 
CGATCCTTCT GCTCCCAAAC CGCGATTCTC CTTGCCTTCA ACTTGATCTT CCTCATGTTC 
ATCTTCCCAG CAATGATCGG AATCGATCTG AGAAGACAGC GAAAAGGAAA AAGAGATTTG 
GCCTACTGCA GTCGAGGAAA CCCACAAATG GCGACTAGTC AAAGTGTGCC GAGCAATGTT 
TCAAATATGT CATCACGTGC CGAGTTGGCT GGCTACGAGA AGCAGGCCGA CGAGTATAAG 
CGTCACGAGC CGTGGTACAC CGTCGGAGGA TTCCTCAACA AGATCTACAT TCCGGCACTC 
AAGAATAATG TTGTTAAGGC TTGCGTTCTC ATCGGAACTA CCACCGCCGT CGTCTTCGGA 
CTCTACGGAA TGTACACCTC GACTCTCGGT CTCGAGCTCG CCGACGTGCT TCCAGAGCAC 
ACCCCACCAG CGGCCTTCCT TCGTGCTCGC GAGCAATACT TCTCCTTCTA CCCGATGTTT 
GCAGTTCTTC GTGGTGACAA GCTTGACATT CCGAACCAAC AGCAACTTAT CGAAGAGTAC 
AGAGCACAGT TGGGATCTTC AAAGTTTATG ATCAAGGCTG AAGGTAAACT TCAACCCTAC 
TGGATGTCTA TGCTCAGAGT CTGGCTGCAA TCTTTGGACA TGGCCTTGGA AAAAGACCTC 
GCCGCTGGCA AATTCGATTT GACAAATGGA AATCCTATCA AGGTGAACGG AGAGAAGCCA 
AGTCCCGAAT CGATGATCGC GGCTAGACTT GTATGCTCCT TCGGCACCAA CTACAACTGT 
GACGGGCGGC TCGGAAAGAT GAAGATGGTG GAAAATGAGG TTATTAATCC AGAAGGATTC 
TATAACTATC TGACGGGATG GTTCAACGTT GACAACATGA TGTACTACGT GTCCCAGGCT 
TCATTCTATC CAACACCACC TGGATGGGAG TATAATGAGA AGCTCGCCAA GGTGGTTCCG 
GCTGCCGAGC CATTGTTGTA CTCACAGATG CCGTTTTATC AGAATGATTT GATCGATACA 
CCAGCGATTG TAAAAATGAT TGAGGAAATC CGTGCCACTT GCGAGGAATA CTCCGAACGA 
GGCCTTTCCA ACCATCCATC GGGTATCGCC TTCACCTTCT GGGAGCAATA TCTTACTTTG 
AGATGGAACT TGTTCCAAGC CATCTGCATC ATCGCGCTCG CCGTGTTCTG TGTCATTTCA 
ATTCTAATGT TCAATCCATG GGCAGCCACT CTTATCATGT GCATCGTAGT GATCACTACC 
ATCGAGCTGG GAGGATTCAT GGGGCTTATG GGCATCAAGA TGAACCCGAT TTCCGCTGTA 
ACCCTCATCT GTGCCGTCGG AATCGGTGTC GAGTTCACTG CTCACGTCGA GTTGGCCTTC 
CTGACAGCTC TAGGAACCAT CGATCAACGC CTGGAGTCTT GCCTCCAGCA CATGTTCGTG 
CCAGTCTATC ACGGAGCCAT CTCAACGTTC CTCGGAGTTG TGATGCTTGT GTTCTCCGAG 
TTCGACTTCG TCGTCACCTA CTTCTTCTAC ACAATGACGT TGCTGGTAGC GTTGGGTGTG 
TTCAACGGGT TGTGTGTTCT TCCAGTGATC CTTACGCTGG TCGGCCCGAA GCCTGAGCTG 
ACACCAACCG ATGGTAGCAG TGTGCTTCCA CCACCTCCAC CATTGCGGCA GCAGTACGCT 
GAGAAGAGTG GAGGTGTGGA AGGTGGGATG AGGAAACGGA AGGAGAAGAG GCCTGCGGAG 
GTCGAAATGT CGGCTCGCGA CTCTCCATCA ACATCCTCAG CGTCCCATTC TTCTGATGAC 
GAGTCATCGC CAGCGCACAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ptc-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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