glp-4 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	glp-4
Entrez Gene ID	173224
Full Name	Valine--tRNA ligase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060976.4	NP_493377.1	Valine--tRNA ligase
NM_060976.5	NP_493377.1	Valine--tRNA ligase

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glp-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glp-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336302	NM_060976.5 Latest version!	Caenorhabditis elegans Valine--tRNA ligase (glp-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa336302	NM_060976.4	Caenorhabditis elegans Valine--tRNA ligase (glp-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glp-4 ( NM_060976.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493377.1 Caenorhabditis elegans Valine--tRNA ligase (glp-4), partial mRNA.

Price & Availability↑

CloneID	OCa336302
Clone ID Related Accession (Same CDS sequence)	NM_060976.4 , NM_060976.5
Accession Version	NM_060976.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Valine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060976.3. COMPLETENESS: incomplete on both ends.

ATGTCGGACC CCGCAGGAAA CCGTCCGAAG ACGGAAAAAG AGCTGAAAAA AGAGGCGGAG 
AAGGCGGCGA AACTCGCGAA ATTCGAGGAA AAGCAGAAAA AGCTTGCCGA GAAGAAGGCT 
GCCGCAAGTG ATAAGCCGGT GAAGGAGGCC AAGGCCAAAA AGGAGCAAAC TGTGGAAGCG 
GCGGAGCCTG TAGATCAGAC TCCAACTGGT CAGAGGAAGA AAATCGACGG AGAAATCCCC 
GCGGCCTACT TCCCAGGATA CGTCGAATCC GGCTGGTACT CTTGGTGGGA AAAGGAGGGA 
TTCTTCAAGC CCGAGTACAT TGACAAGCTG AATCCAGGCT CGAATCCCGC CGACTCGTTC 
ACAGTTTGCA TTCCACCACC AAATGTGACA GGAAATCTAC ACGTTGGACA CGCGCTGGCC 
ACTACCGTCG AGGATACTAT CACCAGATTC AACAGAATGC ACGGAAAACG GACACTTTTT 
AATCCGGGAT GTGATCATGC TGGAATCGCA ACGCAAGTCG TCGTCGAGAA ACGTCTGAAA 
CGAGAACGTG GATTGACACG TCACGATTTG GGACGGGATC GATTCAATCA AGAAGTTTGG 
CACTGGAAGA ATGAGAAAGG GGATGTGATT TATGATCAAT TCCGAAAACT CGGCGCCTCT 
GTCGACTGGG ATCGTGCCGT TTTTACGATG GATCCGAAAA TGTGCCGGGC TGTAACGGAG 
GCTTTTATTC GAATGCACGA GTCTGGAACT ATTTACAGAT CTAATAGACT TGTCAATTGG 
AGTTGCGCGC TGAGATCTGC GATTTCTGAC ATCGAAGTGG ACAAGAAAGA GCTAACCGGA 
AGCACACTGA TCGCCGTGCC CGGATACGAT AAAAAAATCG AATTCGGCGT GCTCAACTCG 
TTCGCCTACA AGATTCAAGG ATCCGACGAG GAGATTGTCG TCTCCACAAC ACGTATCGAA 
ACAATGCTCG GAGATTCAGG AGTTGCTGTT CATCCAGACG ATCAGAGATA CAAGCATCTC 
GTCGGAAAAC AGTGTATTCA TCCATTCATC CCCACCCGAA ACCTCCCGAT CTTTGCCGAC 
TCATTCGTCG AGATGGAATT CGGAACCGGA GCGGTAAAGA TCACTCCAGC ACACGATCAT 
AATGATTATG AAGTCGGAAT TCGTCAAAAC CTTCCATTCC ACAATTGTAT CACAGATGAT 
GGTCTGATCT CTCAGGGATG CGGGGAATTC TCCGGAATGA AGAGATTCGA CGCCAGGACC 
GCCGTTATTG AGGCTCTCAA GGAGAAGGGT CTCTACCGTG GAAAAGAGGA TAATCCGATG 
GTTGTGCCGA CGTGTTCTAG ATCCAAAGAT GTCATTGAGC CTATTCTCAA GCCTCAATGG 
TATGTCAAGT GTGCTCATAT GGCAGAGAAA GCCGTCGCCG CTGTCGCCAA CGGTGATCTA 
CAAATCATTC CGGAATTCCA TAAAGCCACG TGGAATCGGT GGTTGGAGTC GAGCAGAGAT 
TGGTGTATCT CTAGGCAGCT ATGGTGGGGA CACCGGATCC CTGCATACTA TATCTCATTT 
GCCGACGGAA GAGAACAACC TCTGCCAGAG GAGAATTATT GGGTCTCGGC ACGAACTGAA 
CAGGAGGCAC TTGCCAAGGC GGCACAGAAA TTCCAAGTTC CTGAAGCGGA GATCCTGCTG 
AAATGGGATG AGGATGTGCT TGACACGTGG TTTTCGTCTG GAATGTGGCC GTTTGCAGTT 
TTTGGATGGC CTGATGCCAC AAAGGATATG GATTTATTCT TCCCTGGTGC TGTGTTGGAG 
ACTGGACATG ATATTCTCTT CTTTTGGGTT GCAAGAATGG TTTTTATGGC TCAGGAGTTG 
ACTGGAAAAC TTCCGTTTAA AGAGATCCTG CTACACGCAA TGATCCGAGA TGCCCATGGA 
CGTAAAATGT CAAAATCCCT CGGAAATGTG ATTGATCCAC TCGATGTGAT TCGTGGAATC 
TCTCTAAACG ATCTTCAAGC TCAACTTCTC GGCGGAAATC TCGACGAAAA AGAGATTGCA 
GTTGCAAAAG AAGGACAAGC ACGTGATTAT CCAGATGGAA TTCCTGAATG TGGTGTAGAT 
GCTCTTCGAT TTGCACTACT TTCCTATACA TCACAAGGAA GAGATATTAA TTTAGATGTT 
CTCAGAGTTC ATGGATATCG AAAATTCTGT AATAAGCTCT GGCAAGTTGT GAGATTCGCG 
TTGGCACGAA TTTCTGATAA ACCGGAACAA AAACCAACGT TCGAGATTAA TCTGAAATCA 
GCTACACCTA CTGATCTATG GATTCTATCA AGACTCGCAA AAGCTGTCAA AGAGACGAAT 
GAGGCATTGA AGGCATATAA CTTCACTCAG GCAACCACTG TTACCTACAA TTTCTGGTTA 
TACGACTTCT GTGATGTTTA TGTCGAAACT ATCAAGCCTG TGCTTTATGG AGACAACACG 
ACTCTCCGCC AGGTGGCAAT TTCCGTCCTT CACAAATGTA TTGATACAGG ACTTCGTCTT 
ATTTCTCCAT TAATGCCATT CATCTCAGAA GAATTATGGC AACGTATGCC ACGTCTCGAC 
GATTCGGATT ACACGTCACC ATCGATTATT GTTGCACAAT ATCCATTAAC ACAGAAATAT 
GAAAAATATC AAAATGAGAA GCTGGAGGCT GCATTCGAAT TTGCACAGGA GCTCATTGGA 
AAAGTCCGAT CGTTGAGAGC TGATTATGAT TTGAAGAAGA CTAAGATTAC TATGCAAATT 
CTCTCCGAAA CACCAGAAGA CGAGTCAATG CTCAATGATA TATCTGCAGT GATAACCACC 
TTAACATTCA GTGAAAAAGT CTCAATTCTC AATAAATGTG AATCCGATAA AATTGAAAAA 
GGTTCTGCAC ATATTGCTTG TGGAGGACGT TGTCAAGTAT ACATCAATTT GACTGGAATT 
ATCGATGTTC CAAAAGAAAT CGAAAAGCTC GGAGCCAAAT TGCAAAAGAA TCAGATTTCT 
GTGAAAAAGA TTGGAGATAT TCAATCGAGT GCTGATTATG AGCAAAAAGT TCCAGTTGAT 
ATTCGTGCTC TGGATCAGGA AAAGAAAGCA ACCCTGGAGA AGGAAATCGA AAACATCACT 
GCGGCGATTG CACAACTCAA GGCTCTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493377.1
CDS	1..3153
Translation	MSDPAGNRPKTEKELKKEAEKAAKLAKFEEKQKKLAEKKAAASDKPVKEAKAKKEQTVEAAEPVDQTPTGQRKKIDGEIPAAYFPGYVESGWYSWWEKEGFFKPEYIDKLNPGSNPADSFTVCIPPPNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHDLGRDRFNQEVWHWKNEKGDVIYDQFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVDKKELTGSTLIAVPGYDKKIEFGVLNSFAYKIQGSDEEIVVSTTRIETMLGDSGVAVHPDDQRYKHLVGKQCIHPFIPTRNLPIFADSFVEMEFGTGAVKITPAHDHNDYEVGIRQNLPFHNCITDDGLISQGCGEFSGMKRFDARTAVIEALKEKGLYRGKEDNPMVVPTCSRSKDVIEPILKPQWYVKCAHMAEKAVAAVANGDLQIIPEFHKATWNRWLESSRDWCISRQLWWGHRIPAYYISFADGREQPLPEENYWVSARTEQEALAKAAQKFQVPEAEILLKWDEDVLDTWFSSGMWPFAVFGWPDATKDMDLFFPGAVLETGHDILFFWVARMVFMAQELTGKLPFKEILLHAMIRDAHGRKMSKSLGNVIDPLDVIRGISLNDLQAQLLGGNLDEKEIAVAKEGQARDYPDGIPECGVDALRFALLSYTSQGRDINLDVLRVHGYRKFCNKLWQVVRFALARISDKPEQKPTFEINLKSATPTDLWILSRLAKAVKETNEALKAYNFTQATTVTYNFWLYDFCDVYVETIKPVLYGDNTTLRQVAISVLHKCIDTGLRLISPLMPFISEELWQRMPRLDDSDYTSPSIIVAQYPLTQKYEKYQNEKLEAAFEFAQELIGKVRSLRADYDLKKTKITMQILSETPEDESMLNDISAVITTLTFSEKVSILNKCESDKIEKGSAHIACGGRCQVYINLTGIIDVPKEIEKLGAKLQKNQISVKKIGDIQSSADYEQKVPVDIRALDQEKKATLEKEIENITAAIAQLKALN

Target ORF information:

RefSeq Version	NM_060976.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Valine--tRNA ligase (glp-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060976.4

ORF Insert Sequence:

ATGTCGGACC CCGCAGGAAA CCGTCCGAAG ACGGAAAAAG AGCTGAAAAA AGAGGCGGAG 
AAGGCGGCGA AACTCGCGAA ATTCGAGGAA AAGCAGAAAA AGCTTGCCGA GAAGAAGGCT 
GCCGCAAGTG ATAAGCCGGT GAAGGAGGCC AAGGCCAAAA AGGAGCAAAC TGTGGAAGCG 
GCGGAGCCTG TAGATCAGAC TCCAACTGGT CAGAGGAAGA AAATCGACGG AGAAATCCCC 
GCGGCCTACT TCCCAGGATA CGTCGAATCC GGCTGGTACT CTTGGTGGGA AAAGGAGGGA 
TTCTTCAAGC CCGAGTACAT TGACAAGCTG AATCCAGGCT CGAATCCCGC CGACTCGTTC 
ACAGTTTGCA TTCCACCACC AAATGTGACA GGAAATCTAC ACGTTGGACA CGCGCTGGCC 
ACTACCGTCG AGGATACTAT CACCAGATTC AACAGAATGC ACGGAAAACG GACACTTTTT 
AATCCGGGAT GTGATCATGC TGGAATCGCA ACGCAAGTCG TCGTCGAGAA ACGTCTGAAA 
CGAGAACGTG GATTGACACG TCACGATTTG GGACGGGATC GATTCAATCA AGAAGTTTGG 
CACTGGAAGA ATGAGAAAGG GGATGTGATT TATGATCAAT TCCGAAAACT CGGCGCCTCT 
GTCGACTGGG ATCGTGCCGT TTTTACGATG GATCCGAAAA TGTGCCGGGC TGTAACGGAG 
GCTTTTATTC GAATGCACGA GTCTGGAACT ATTTACAGAT CTAATAGACT TGTCAATTGG 
AGTTGCGCGC TGAGATCTGC GATTTCTGAC ATCGAAGTGG ACAAGAAAGA GCTAACCGGA 
AGCACACTGA TCGCCGTGCC CGGATACGAT AAAAAAATCG AATTCGGCGT GCTCAACTCG 
TTCGCCTACA AGATTCAAGG ATCCGACGAG GAGATTGTCG TCTCCACAAC ACGTATCGAA 
ACAATGCTCG GAGATTCAGG AGTTGCTGTT CATCCAGACG ATCAGAGATA CAAGCATCTC 
GTCGGAAAAC AGTGTATTCA TCCATTCATC CCCACCCGAA ACCTCCCGAT CTTTGCCGAC 
TCATTCGTCG AGATGGAATT CGGAACCGGA GCGGTAAAGA TCACTCCAGC ACACGATCAT 
AATGATTATG AAGTCGGAAT TCGTCAAAAC CTTCCATTCC ACAATTGTAT CACAGATGAT 
GGTCTGATCT CTCAGGGATG CGGGGAATTC TCCGGAATGA AGAGATTCGA CGCCAGGACC 
GCCGTTATTG AGGCTCTCAA GGAGAAGGGT CTCTACCGTG GAAAAGAGGA TAATCCGATG 
GTTGTGCCGA CGTGTTCTAG ATCCAAAGAT GTCATTGAGC CTATTCTCAA GCCTCAATGG 
TATGTCAAGT GTGCTCATAT GGCAGAGAAA GCCGTCGCCG CTGTCGCCAA CGGTGATCTA 
CAAATCATTC CGGAATTCCA TAAAGCCACG TGGAATCGGT GGTTGGAGTC GAGCAGAGAT 
TGGTGTATCT CTAGGCAGCT ATGGTGGGGA CACCGGATCC CTGCATACTA TATCTCATTT 
GCCGACGGAA GAGAACAACC TCTGCCAGAG GAGAATTATT GGGTCTCGGC ACGAACTGAA 
CAGGAGGCAC TTGCCAAGGC GGCACAGAAA TTCCAAGTTC CTGAAGCGGA GATCCTGCTG 
AAATGGGATG AGGATGTGCT TGACACGTGG TTTTCGTCTG GAATGTGGCC GTTTGCAGTT 
TTTGGATGGC CTGATGCCAC AAAGGATATG GATTTATTCT TCCCTGGTGC TGTGTTGGAG 
ACTGGACATG ATATTCTCTT CTTTTGGGTT GCAAGAATGG TTTTTATGGC TCAGGAGTTG 
ACTGGAAAAC TTCCGTTTAA AGAGATCCTG CTACACGCAA TGATCCGAGA TGCCCATGGA 
CGTAAAATGT CAAAATCCCT CGGAAATGTG ATTGATCCAC TCGATGTGAT TCGTGGAATC 
TCTCTAAACG ATCTTCAAGC TCAACTTCTC GGCGGAAATC TCGACGAAAA AGAGATTGCA 
GTTGCAAAAG AAGGACAAGC ACGTGATTAT CCAGATGGAA TTCCTGAATG TGGTGTAGAT 
GCTCTTCGAT TTGCACTACT TTCCTATACA TCACAAGGAA GAGATATTAA TTTAGATGTT 
CTCAGAGTTC ATGGATATCG AAAATTCTGT AATAAGCTCT GGCAAGTTGT GAGATTCGCG 
TTGGCACGAA TTTCTGATAA ACCGGAACAA AAACCAACGT TCGAGATTAA TCTGAAATCA 
GCTACACCTA CTGATCTATG GATTCTATCA AGACTCGCAA AAGCTGTCAA AGAGACGAAT 
GAGGCATTGA AGGCATATAA CTTCACTCAG GCAACCACTG TTACCTACAA TTTCTGGTTA 
TACGACTTCT GTGATGTTTA TGTCGAAACT ATCAAGCCTG TGCTTTATGG AGACAACACG 
ACTCTCCGCC AGGTGGCAAT TTCCGTCCTT CACAAATGTA TTGATACAGG ACTTCGTCTT 
ATTTCTCCAT TAATGCCATT CATCTCAGAA GAATTATGGC AACGTATGCC ACGTCTCGAC 
GATTCGGATT ACACGTCACC ATCGATTATT GTTGCACAAT ATCCATTAAC ACAGAAATAT 
GAAAAATATC AAAATGAGAA GCTGGAGGCT GCATTCGAAT TTGCACAGGA GCTCATTGGA 
AAAGTCCGAT CGTTGAGAGC TGATTATGAT TTGAAGAAGA CTAAGATTAC TATGCAAATT 
CTCTCCGAAA CACCAGAAGA CGAGTCAATG CTCAATGATA TATCTGCAGT GATAACCACC 
TTAACATTCA GTGAAAAAGT CTCAATTCTC AATAAATGTG AATCCGATAA AATTGAAAAA 
GGTTCTGCAC ATATTGCTTG TGGAGGACGT TGTCAAGTAT ACATCAATTT GACTGGAATT 
ATCGATGTTC CAAAAGAAAT CGAAAAGCTC GGAGCCAAAT TGCAAAAGAA TCAGATTTCT 
GTGAAAAAGA TTGGAGATAT TCAATCGAGT GCTGATTATG AGCAAAAAGT TCCAGTTGAT 
ATTCGTGCTC TGGATCAGGA AAAGAAAGCA ACCCTGGAGA AGGAAATCGA AAACATCACT 
GCGGCGATTG CACAACTCAA GGCTCTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glp-4 ( NM_060976.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493377.1 Caenorhabditis elegans Valine--tRNA ligase (glp-4), mRNA.

Price & Availability↑

CloneID	OCa336302
Clone ID Related Accession (Same CDS sequence)	NM_060976.4 , NM_060976.5
Accession Version	NM_060976.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Valine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060976.4.

ATGTCGGACC CCGCAGGAAA CCGTCCGAAG ACGGAAAAAG AGCTGAAAAA AGAGGCGGAG 
AAGGCGGCGA AACTCGCGAA ATTCGAGGAA AAGCAGAAAA AGCTTGCCGA GAAGAAGGCT 
GCCGCAAGTG ATAAGCCGGT GAAGGAGGCC AAGGCCAAAA AGGAGCAAAC TGTGGAAGCG 
GCGGAGCCTG TAGATCAGAC TCCAACTGGT CAGAGGAAGA AAATCGACGG AGAAATCCCC 
GCGGCCTACT TCCCAGGATA CGTCGAATCC GGCTGGTACT CTTGGTGGGA AAAGGAGGGA 
TTCTTCAAGC CCGAGTACAT TGACAAGCTG AATCCAGGCT CGAATCCCGC CGACTCGTTC 
ACAGTTTGCA TTCCACCACC AAATGTGACA GGAAATCTAC ACGTTGGACA CGCGCTGGCC 
ACTACCGTCG AGGATACTAT CACCAGATTC AACAGAATGC ACGGAAAACG GACACTTTTT 
AATCCGGGAT GTGATCATGC TGGAATCGCA ACGCAAGTCG TCGTCGAGAA ACGTCTGAAA 
CGAGAACGTG GATTGACACG TCACGATTTG GGACGGGATC GATTCAATCA AGAAGTTTGG 
CACTGGAAGA ATGAGAAAGG GGATGTGATT TATGATCAAT TCCGAAAACT CGGCGCCTCT 
GTCGACTGGG ATCGTGCCGT TTTTACGATG GATCCGAAAA TGTGCCGGGC TGTAACGGAG 
GCTTTTATTC GAATGCACGA GTCTGGAACT ATTTACAGAT CTAATAGACT TGTCAATTGG 
AGTTGCGCGC TGAGATCTGC GATTTCTGAC ATCGAAGTGG ACAAGAAAGA GCTAACCGGA 
AGCACACTGA TCGCCGTGCC CGGATACGAT AAAAAAATCG AATTCGGCGT GCTCAACTCG 
TTCGCCTACA AGATTCAAGG ATCCGACGAG GAGATTGTCG TCTCCACAAC ACGTATCGAA 
ACAATGCTCG GAGATTCAGG AGTTGCTGTT CATCCAGACG ATCAGAGATA CAAGCATCTC 
GTCGGAAAAC AGTGTATTCA TCCATTCATC CCCACCCGAA ACCTCCCGAT CTTTGCCGAC 
TCATTCGTCG AGATGGAATT CGGAACCGGA GCGGTAAAGA TCACTCCAGC ACACGATCAT 
AATGATTATG AAGTCGGAAT TCGTCAAAAC CTTCCATTCC ACAATTGTAT CACAGATGAT 
GGTCTGATCT CTCAGGGATG CGGGGAATTC TCCGGAATGA AGAGATTCGA CGCCAGGACC 
GCCGTTATTG AGGCTCTCAA GGAGAAGGGT CTCTACCGTG GAAAAGAGGA TAATCCGATG 
GTTGTGCCGA CGTGTTCTAG ATCCAAAGAT GTCATTGAGC CTATTCTCAA GCCTCAATGG 
TATGTCAAGT GTGCTCATAT GGCAGAGAAA GCCGTCGCCG CTGTCGCCAA CGGTGATCTA 
CAAATCATTC CGGAATTCCA TAAAGCCACG TGGAATCGGT GGTTGGAGTC GAGCAGAGAT 
TGGTGTATCT CTAGGCAGCT ATGGTGGGGA CACCGGATCC CTGCATACTA TATCTCATTT 
GCCGACGGAA GAGAACAACC TCTGCCAGAG GAGAATTATT GGGTCTCGGC ACGAACTGAA 
CAGGAGGCAC TTGCCAAGGC GGCACAGAAA TTCCAAGTTC CTGAAGCGGA GATCCTGCTG 
AAATGGGATG AGGATGTGCT TGACACGTGG TTTTCGTCTG GAATGTGGCC GTTTGCAGTT 
TTTGGATGGC CTGATGCCAC AAAGGATATG GATTTATTCT TCCCTGGTGC TGTGTTGGAG 
ACTGGACATG ATATTCTCTT CTTTTGGGTT GCAAGAATGG TTTTTATGGC TCAGGAGTTG 
ACTGGAAAAC TTCCGTTTAA AGAGATCCTG CTACACGCAA TGATCCGAGA TGCCCATGGA 
CGTAAAATGT CAAAATCCCT CGGAAATGTG ATTGATCCAC TCGATGTGAT TCGTGGAATC 
TCTCTAAACG ATCTTCAAGC TCAACTTCTC GGCGGAAATC TCGACGAAAA AGAGATTGCA 
GTTGCAAAAG AAGGACAAGC ACGTGATTAT CCAGATGGAA TTCCTGAATG TGGTGTAGAT 
GCTCTTCGAT TTGCACTACT TTCCTATACA TCACAAGGAA GAGATATTAA TTTAGATGTT 
CTCAGAGTTC ATGGATATCG AAAATTCTGT AATAAGCTCT GGCAAGTTGT GAGATTCGCG 
TTGGCACGAA TTTCTGATAA ACCGGAACAA AAACCAACGT TCGAGATTAA TCTGAAATCA 
GCTACACCTA CTGATCTATG GATTCTATCA AGACTCGCAA AAGCTGTCAA AGAGACGAAT 
GAGGCATTGA AGGCATATAA CTTCACTCAG GCAACCACTG TTACCTACAA TTTCTGGTTA 
TACGACTTCT GTGATGTTTA TGTCGAAACT ATCAAGCCTG TGCTTTATGG AGACAACACG 
ACTCTCCGCC AGGTGGCAAT TTCCGTCCTT CACAAATGTA TTGATACAGG ACTTCGTCTT 
ATTTCTCCAT TAATGCCATT CATCTCAGAA GAATTATGGC AACGTATGCC ACGTCTCGAC 
GATTCGGATT ACACGTCACC ATCGATTATT GTTGCACAAT ATCCATTAAC ACAGAAATAT 
GAAAAATATC AAAATGAGAA GCTGGAGGCT GCATTCGAAT TTGCACAGGA GCTCATTGGA 
AAAGTCCGAT CGTTGAGAGC TGATTATGAT TTGAAGAAGA CTAAGATTAC TATGCAAATT 
CTCTCCGAAA CACCAGAAGA CGAGTCAATG CTCAATGATA TATCTGCAGT GATAACCACC 
TTAACATTCA GTGAAAAAGT CTCAATTCTC AATAAATGTG AATCCGATAA AATTGAAAAA 
GGTTCTGCAC ATATTGCTTG TGGAGGACGT TGTCAAGTAT ACATCAATTT GACTGGAATT 
ATCGATGTTC CAAAAGAAAT CGAAAAGCTC GGAGCCAAAT TGCAAAAGAA TCAGATTTCT 
GTGAAAAAGA TTGGAGATAT TCAATCGAGT GCTGATTATG AGCAAAAAGT TCCAGTTGAT 
ATTCGTGCTC TGGATCAGGA AAAGAAAGCA ACCCTGGAGA AGGAAATCGA AAACATCACT 
GCGGCGATTG CACAACTCAA GGCTCTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493377.1
CDS	18..3170
Translation	MSDPAGNRPKTEKELKKEAEKAAKLAKFEEKQKKLAEKKAAASDKPVKEAKAKKEQTVEAAEPVDQTPTGQRKKIDGEIPAAYFPGYVESGWYSWWEKEGFFKPEYIDKLNPGSNPADSFTVCIPPPNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHDLGRDRFNQEVWHWKNEKGDVIYDQFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVDKKELTGSTLIAVPGYDKKIEFGVLNSFAYKIQGSDEEIVVSTTRIETMLGDSGVAVHPDDQRYKHLVGKQCIHPFIPTRNLPIFADSFVEMEFGTGAVKITPAHDHNDYEVGIRQNLPFHNCITDDGLISQGCGEFSGMKRFDARTAVIEALKEKGLYRGKEDNPMVVPTCSRSKDVIEPILKPQWYVKCAHMAEKAVAAVANGDLQIIPEFHKATWNRWLESSRDWCISRQLWWGHRIPAYYISFADGREQPLPEENYWVSARTEQEALAKAAQKFQVPEAEILLKWDEDVLDTWFSSGMWPFAVFGWPDATKDMDLFFPGAVLETGHDILFFWVARMVFMAQELTGKLPFKEILLHAMIRDAHGRKMSKSLGNVIDPLDVIRGISLNDLQAQLLGGNLDEKEIAVAKEGQARDYPDGIPECGVDALRFALLSYTSQGRDINLDVLRVHGYRKFCNKLWQVVRFALARISDKPEQKPTFEINLKSATPTDLWILSRLAKAVKETNEALKAYNFTQATTVTYNFWLYDFCDVYVETIKPVLYGDNTTLRQVAISVLHKCIDTGLRLISPLMPFISEELWQRMPRLDDSDYTSPSIIVAQYPLTQKYEKYQNEKLEAAFEFAQELIGKVRSLRADYDLKKTKITMQILSETPEDESMLNDISAVITTLTFSEKVSILNKCESDKIEKGSAHIACGGRCQVYINLTGIIDVPKEIEKLGAKLQKNQISVKKIGDIQSSADYEQKVPVDIRALDQEKKATLEKEIENITAAIAQLKALN

Target ORF information:

RefSeq Version	NM_060976.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Valine--tRNA ligase (glp-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060976.5

ORF Insert Sequence:

ATGTCGGACC CCGCAGGAAA CCGTCCGAAG ACGGAAAAAG AGCTGAAAAA AGAGGCGGAG 
AAGGCGGCGA AACTCGCGAA ATTCGAGGAA AAGCAGAAAA AGCTTGCCGA GAAGAAGGCT 
GCCGCAAGTG ATAAGCCGGT GAAGGAGGCC AAGGCCAAAA AGGAGCAAAC TGTGGAAGCG 
GCGGAGCCTG TAGATCAGAC TCCAACTGGT CAGAGGAAGA AAATCGACGG AGAAATCCCC 
GCGGCCTACT TCCCAGGATA CGTCGAATCC GGCTGGTACT CTTGGTGGGA AAAGGAGGGA 
TTCTTCAAGC CCGAGTACAT TGACAAGCTG AATCCAGGCT CGAATCCCGC CGACTCGTTC 
ACAGTTTGCA TTCCACCACC AAATGTGACA GGAAATCTAC ACGTTGGACA CGCGCTGGCC 
ACTACCGTCG AGGATACTAT CACCAGATTC AACAGAATGC ACGGAAAACG GACACTTTTT 
AATCCGGGAT GTGATCATGC TGGAATCGCA ACGCAAGTCG TCGTCGAGAA ACGTCTGAAA 
CGAGAACGTG GATTGACACG TCACGATTTG GGACGGGATC GATTCAATCA AGAAGTTTGG 
CACTGGAAGA ATGAGAAAGG GGATGTGATT TATGATCAAT TCCGAAAACT CGGCGCCTCT 
GTCGACTGGG ATCGTGCCGT TTTTACGATG GATCCGAAAA TGTGCCGGGC TGTAACGGAG 
GCTTTTATTC GAATGCACGA GTCTGGAACT ATTTACAGAT CTAATAGACT TGTCAATTGG 
AGTTGCGCGC TGAGATCTGC GATTTCTGAC ATCGAAGTGG ACAAGAAAGA GCTAACCGGA 
AGCACACTGA TCGCCGTGCC CGGATACGAT AAAAAAATCG AATTCGGCGT GCTCAACTCG 
TTCGCCTACA AGATTCAAGG ATCCGACGAG GAGATTGTCG TCTCCACAAC ACGTATCGAA 
ACAATGCTCG GAGATTCAGG AGTTGCTGTT CATCCAGACG ATCAGAGATA CAAGCATCTC 
GTCGGAAAAC AGTGTATTCA TCCATTCATC CCCACCCGAA ACCTCCCGAT CTTTGCCGAC 
TCATTCGTCG AGATGGAATT CGGAACCGGA GCGGTAAAGA TCACTCCAGC ACACGATCAT 
AATGATTATG AAGTCGGAAT TCGTCAAAAC CTTCCATTCC ACAATTGTAT CACAGATGAT 
GGTCTGATCT CTCAGGGATG CGGGGAATTC TCCGGAATGA AGAGATTCGA CGCCAGGACC 
GCCGTTATTG AGGCTCTCAA GGAGAAGGGT CTCTACCGTG GAAAAGAGGA TAATCCGATG 
GTTGTGCCGA CGTGTTCTAG ATCCAAAGAT GTCATTGAGC CTATTCTCAA GCCTCAATGG 
TATGTCAAGT GTGCTCATAT GGCAGAGAAA GCCGTCGCCG CTGTCGCCAA CGGTGATCTA 
CAAATCATTC CGGAATTCCA TAAAGCCACG TGGAATCGGT GGTTGGAGTC GAGCAGAGAT 
TGGTGTATCT CTAGGCAGCT ATGGTGGGGA CACCGGATCC CTGCATACTA TATCTCATTT 
GCCGACGGAA GAGAACAACC TCTGCCAGAG GAGAATTATT GGGTCTCGGC ACGAACTGAA 
CAGGAGGCAC TTGCCAAGGC GGCACAGAAA TTCCAAGTTC CTGAAGCGGA GATCCTGCTG 
AAATGGGATG AGGATGTGCT TGACACGTGG TTTTCGTCTG GAATGTGGCC GTTTGCAGTT 
TTTGGATGGC CTGATGCCAC AAAGGATATG GATTTATTCT TCCCTGGTGC TGTGTTGGAG 
ACTGGACATG ATATTCTCTT CTTTTGGGTT GCAAGAATGG TTTTTATGGC TCAGGAGTTG 
ACTGGAAAAC TTCCGTTTAA AGAGATCCTG CTACACGCAA TGATCCGAGA TGCCCATGGA 
CGTAAAATGT CAAAATCCCT CGGAAATGTG ATTGATCCAC TCGATGTGAT TCGTGGAATC 
TCTCTAAACG ATCTTCAAGC TCAACTTCTC GGCGGAAATC TCGACGAAAA AGAGATTGCA 
GTTGCAAAAG AAGGACAAGC ACGTGATTAT CCAGATGGAA TTCCTGAATG TGGTGTAGAT 
GCTCTTCGAT TTGCACTACT TTCCTATACA TCACAAGGAA GAGATATTAA TTTAGATGTT 
CTCAGAGTTC ATGGATATCG AAAATTCTGT AATAAGCTCT GGCAAGTTGT GAGATTCGCG 
TTGGCACGAA TTTCTGATAA ACCGGAACAA AAACCAACGT TCGAGATTAA TCTGAAATCA 
GCTACACCTA CTGATCTATG GATTCTATCA AGACTCGCAA AAGCTGTCAA AGAGACGAAT 
GAGGCATTGA AGGCATATAA CTTCACTCAG GCAACCACTG TTACCTACAA TTTCTGGTTA 
TACGACTTCT GTGATGTTTA TGTCGAAACT ATCAAGCCTG TGCTTTATGG AGACAACACG 
ACTCTCCGCC AGGTGGCAAT TTCCGTCCTT CACAAATGTA TTGATACAGG ACTTCGTCTT 
ATTTCTCCAT TAATGCCATT CATCTCAGAA GAATTATGGC AACGTATGCC ACGTCTCGAC 
GATTCGGATT ACACGTCACC ATCGATTATT GTTGCACAAT ATCCATTAAC ACAGAAATAT 
GAAAAATATC AAAATGAGAA GCTGGAGGCT GCATTCGAAT TTGCACAGGA GCTCATTGGA 
AAAGTCCGAT CGTTGAGAGC TGATTATGAT TTGAAGAAGA CTAAGATTAC TATGCAAATT 
CTCTCCGAAA CACCAGAAGA CGAGTCAATG CTCAATGATA TATCTGCAGT GATAACCACC 
TTAACATTCA GTGAAAAAGT CTCAATTCTC AATAAATGTG AATCCGATAA AATTGAAAAA 
GGTTCTGCAC ATATTGCTTG TGGAGGACGT TGTCAAGTAT ACATCAATTT GACTGGAATT 
ATCGATGTTC CAAAAGAAAT CGAAAAGCTC GGAGCCAAAT TGCAAAAGAA TCAGATTTCT 
GTGAAAAAGA TTGGAGATAT TCAATCGAGT GCTGATTATG AGCAAAAAGT TCCAGTTGAT 
ATTCGTGCTC TGGATCAGGA AAAGAAAGCA ACCCTGGAGA AGGAAATCGA AAACATCACT 
GCGGCGATTG CACAACTCAA GGCTCTCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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glp-4 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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