rtel-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rtel-1
Entrez Gene ID	172946
Full Name	Regulator of telomere elongation helicase 1 homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060368.5	NP_492769.1	Regulator of telomere elongation helicase 1 homolog
NM_060368.4	NP_492769.1	Regulator of telomere elongation helicase 1 homolog

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rtel1	NP_001001882.3
Drosophila melanogaster (fruit fly)	CG4078	NP_572254.1
Arabidopsis thaliana (thale cress)	AT1G79950	NP_178113.3
Oryza sativa (rice)	Os01g0592900	NP_001043456.2
Gallus gallus (chicken)	RTEL1	XP_004947187.1
Danio rerio (zebrafish)	rtel1	NP_001013328.2
Anopheles gambiae (African malaria mosquito)	RTEL1_ANOGA	XP_311162.5
	LOC719519	XP_001113864.2
Canis lupus familiaris (dog)	RTEL1	XP_005635362.1
Bos taurus (cattle)	RTEL1	NP_001091044.1
Caenorhabditis elegans (roundworm)	rtel-1	NP_492769.1
Homo sapiens (human)	RTEL1	NP_001269938.1
Pan troglodytes (chimpanzee)	RTEL1	XP_003953846.1
Rattus norvegicus (Norway rat)	Rtel1	NP_001178786.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analyses of jmjd-5 genetic interactions with genes required for resolving recombination intermediates (rtel-1) or promoting the resolution of RAD-51 double stranded DNA filaments (rfs-1 and helq-1) suggest that jmjd-5 prevents the formation of stalled postsynaptic recombination intermediates and favors RAD-51 removal.
Title: JMJD-5/KDM8 regulates H3K36me2 and is required for late steps of homologous recombination and genome integrity.

findings show that RTEL-1 is required to prevent excess meiotic crossovers, probably by promoting meiotic synthesis-dependent strand annealing
Title: RTEL-1 enforces meiotic crossover interference and homeostasis.

Study finds that rtel-1 mutant worms and RTEL1-depleted human cells share characteristic phenotypes with yeast srs2 mutants: lethality upon deletion of the sgs1/BLM homolog, hyperrecombination, and DNA damage sensitivity.
Title: RTEL1 maintains genomic stability by suppressing homologous recombination.

The following rtel-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rtel-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335668	NM_060368.5 Latest version!	Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa335668	NM_060368.4	Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rtel-1 ( NM_060368.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492769.1 Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), mRNA.

Price & Availability↑

CloneID	OCa335668
Clone ID Related Accession (Same CDS sequence)	NM_060368.5 , NM_060368.4
Accession Version	NM_060368.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Regulator of telomere elongation helicase 1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060368.4.

ATGCCGCCGA AAGCTTCAAA TTCAACGGAA GTTTGGATTA ACCCGAAGTT AAGTGTAAAA 
TTCCCATTTG AACCATACGA ATGTCAGAGG ATCTTCATGA AGAACGTTGT CGATGTACTG 
GATAGAAAAC TAGACGCAGC ACTTGAAAGT CCCACGGGTA CCGGAAAAAC GCTGTCTCTT 
CTCTGTTCGA CGCTCGCATG GGTTCAACGT CAAAAAGAGA CGAAGCCGCT GGATTTTGCC 
ACGTGGCAGA CGTCAGGAGC CGGTGGAGCA GAAAAAACTG ATGAGAAATT GAAGAGTGCA 
TACGTTCCGA CGATATTTTA TGCATCAAGA ACACATTCAC AGTTGGAACA AGTTGTTCAT 
GAATTGAATA GAACTGAATA TAAATGGGTC AAAACCACAA TTCTAGGATC CCGTGAACAC 
TTCTGCATCA ATCAAAAAGT CAAGAAAATC AAAGAATCCA ATCGACAGGC TCATGTTTGT 
CGTGGATTGG TCTCGAAGCG AGCATGTCAT TATTATAATA AATTTGACGC GTGCACCACA 
GACAAAATGA CAGAATTTTT GGATAAAGGC GAAGCAATGG ATATTGAAGA TTTCGTGAAA 
CTTGGAACTC AAAATTCATT ATGCCCATAT TTCATGTCTC GGCAACGTTC TGAAACTGCT 
GAACTCATTC TTCTTCCCTA CAACTACATT ATCGATCCAA AAATGAGACG AAGATACAAG 
CTGGACCTGA AAAATTCAAT TGTGATCTTC GATGAGGCTC ATAATCTAGA ATCAATTTGC 
GAATCCAATG CATCTGCTGA GCTCACTTCC ACGTCAATTG CTCTTTGTAT TGAAGAGCTG 
AAAAAGGTTC TGGCGTTGTT GGTAGATGAA GAAGAGACTG CAAGAAGTGA AGCAGATGCT 
GAAACTGGAG CATTCGGAAG TGCAAAAATT GATTTAACAA AGAAATTGAT TGAGAATTTG 
AGAACTGAAG ATTTGATGAC AGTTTTAGAG AAAATATTTT CATTGGAAGA AGAAATGGAT 
AAATTATTTG GATCATCTCA ATTAAAATCT GTACCACCAT TGAGTGGAAA AGCAAGTGAC 
GGTGAAATTC TTCTTGAAAC TCTTGCAAAA GCTGGATTCG ATGCAAATTC TGTTGAACGG 
CTTGTTGATG TTCTTCGAGA TGCCATTTCT TATCTTTTGT CTAAAAATGA AGAAGTCGCT 
CTAACCGAAA AAGGAGATGG AATGGAAAAA GTGGCGGATT TCTTGTTGTC CATCTATTCG 
ACACACGCTC AAGATGTTGC TGCTGCTGTT GGCGAGGAAA CTGTTAAACT AGTTGATCGA 
GTTGATCCAA AAACTGTGGC TAGAAATTGC AAATTGTATA TTCAAAAGGA TAAGGACAAT 
GAGAAGCTTA CAATCAAATA TTTCTGTTTC CAAGCTTCAA TCTCGATGAG AATGCTCAAA 
ATGCGAGGAG TTCGAAATGT TCTTCTGGCC AGTGGAACCC TTTCACCAAT ACAGGCGTTC 
ACTTATAATA TGGGATTGAA TTTCGGTGCA ATTCTTGAGA ATGAGCATGC TCTGAAGCAA 
GTTCCAGTTT TAACTTCAAT TGTGACACGT GGAAAACGAG GAGGACTCGC CGGTTCATTC 
CAAAATCGAA AAAATCTGGA TTACGTGACA GGTGTAGCAG AAGCATTGTT ACGTGTCATG 
GAAGTTATTC CGCAAGGAAT TCTCATTTTC TTCTCATCAT ATTCTCAAAT GGATGAACTT 
GTTGCGACGT GGAAAACGAC GAAATGGTCG AGTAATTCGA ATGAATCGTT TTGGGAAAAA 
ATGGAGAAGA CGAAGAGAGT TGTGGTAGAA CCAAGAGCTA AAGAAGAACT AGCTGCTATT 
CGTCTGAGAT ATACACAAGG CGTCTCTGAA CAACACGGCG CTGCTCTATT AGCAGTTTGT 
CGTGGAAAAG TATCAGAAGG AATCGATTTT TGTGATGCCG AATCAAGAGC CGTCATTATT 
ATTGGAATCC CATATCCACC GATTCATGAT GAGAGAGTTG TTTTGAAGAA AATGTATTTG 
GACGATCTGA TGGGCAGAAA AGACACGAAA TCTGAACGTC AATCGTCTCA AGATTGGTAT 
CAAATGGAAG CGTTTAGAGC TGTAAATCAA GCAATCGGCC GAGTTCTTCG TCATAAGGAT 
GATTTTGGAA CTGTTGTTCT GATGGATACA CGTTATGCTT CAGCAAAACC TGAAATGTTT 
CCGAAATGGC TCAGAAATAC GATTTCTCGG AGTGACACTG ATGGGTGCGC TCTCAAGACA 
TCACGATTTT TCAAAGAAAG AGGACATTTG ATTGAGAATT CGAAGACCGA ATACATAAAA 
AAGCAGGCAA AACAGTGCAA ATCGTTCCGA CAAGTGAAGC AAACAGCTGC ATCAGATTCC 
AAAGATGATA TTATTGAAAT CACATTAGAA GATATGTTCT CGCCTGCAAA TATGAAATTG 
GAAAAGAAAG AAATCACTAA ATTACGTCCT CCACAATTGA TACCTTCAAC TTCTTCAGTT 
TTCAGCCTAC CAACGAACGA AGATGAGCTG AAAATCAAAA AATGGGAGCA AGAAAACGAT 
ATTCAATGCT TATCTTCAAT ACCCCTTGAA TCTAATAAAC GAAAGTTTAA AATTGAAACA 
CCAGGGCCAT CGACATCTAC GCTGACACAG AAAAGTGAAC CACCAAAAAA GAAGAAAATT 
CTGCTTTTGA CACGAAATAC TCTACCTGAT GAATATCAAA AAGCAATTGA AATTCCAACT 
TCTGAACTTT TGAAAGATAT GTCTGACGAC AATAAAAAGC AATTTGCCAC CACACTCAGA 
AGCTACAAAG CTGAAAGTAT CAGATGGGAT GAAGTTTTTC AAAGATTTCG GCCAATTTTT 
GTTCCGCACA AGGCTGATTT GTTTATTGCT TGCTCCAACG TTCTCCGATC GGAAGACAAA 
ATGAAATATC TGAAAAAGGC GCTTGAATCG AAGATCCACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492769.1
CDS	2..2986
Translation	MPPKASNSTEVWINPKLSVKFPFEPYECQRIFMKNVVDVLDRKLDAALESPTGTGKTLSLLCSTLAWVQRQKETKPLDFATWQTSGAGGAEKTDEKLKSAYVPTIFYASRTHSQLEQVVHELNRTEYKWVKTTILGSREHFCINQKVKKIKESNRQAHVCRGLVSKRACHYYNKFDACTTDKMTEFLDKGEAMDIEDFVKLGTQNSLCPYFMSRQRSETAELILLPYNYIIDPKMRRRYKLDLKNSIVIFDEAHNLESICESNASAELTSTSIALCIEELKKVLALLVDEEETARSEADAETGAFGSAKIDLTKKLIENLRTEDLMTVLEKIFSLEEEMDKLFGSSQLKSVPPLSGKASDGEILLETLAKAGFDANSVERLVDVLRDAISYLLSKNEEVALTEKGDGMEKVADFLLSIYSTHAQDVAAAVGEETVKLVDRVDPKTVARNCKLYIQKDKDNEKLTIKYFCFQASISMRMLKMRGVRNVLLASGTLSPIQAFTYNMGLNFGAILENEHALKQVPVLTSIVTRGKRGGLAGSFQNRKNLDYVTGVAEALLRVMEVIPQGILIFFSSYSQMDELVATWKTTKWSSNSNESFWEKMEKTKRVVVEPRAKEELAAIRLRYTQGVSEQHGAALLAVCRGKVSEGIDFCDAESRAVIIIGIPYPPIHDERVVLKKMYLDDLMGRKDTKSERQSSQDWYQMEAFRAVNQAIGRVLRHKDDFGTVVLMDTRYASAKPEMFPKWLRNTISRSDTDGCALKTSRFFKERGHLIENSKTEYIKKQAKQCKSFRQVKQTAASDSKDDIIEITLEDMFSPANMKLEKKEITKLRPPQLIPSTSSVFSLPTNEDELKIKKWEQENDIQCLSSIPLESNKRKFKIETPGPSTSTLTQKSEPPKKKKILLLTRNTLPDEYQKAIEIPTSELLKDMSDDNKKQFATTLRSYKAESIRWDEVFQRFRPIFVPHKADLFIACSNVLRSEDKMKYLKKALESKIHT

Target ORF information:

RefSeq Version	NM_060368.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060368.5

ORF Insert Sequence:

ATGCCGCCGA AAGCTTCAAA TTCAACGGAA GTTTGGATTA ACCCGAAGTT AAGTGTAAAA 
TTCCCATTTG AACCATACGA ATGTCAGAGG ATCTTCATGA AGAACGTTGT CGATGTACTG 
GATAGAAAAC TAGACGCAGC ACTTGAAAGT CCCACGGGTA CCGGAAAAAC GCTGTCTCTT 
CTCTGTTCGA CGCTCGCATG GGTTCAACGT CAAAAAGAGA CGAAGCCGCT GGATTTTGCC 
ACGTGGCAGA CGTCAGGAGC CGGTGGAGCA GAAAAAACTG ATGAGAAATT GAAGAGTGCA 
TACGTTCCGA CGATATTTTA TGCATCAAGA ACACATTCAC AGTTGGAACA AGTTGTTCAT 
GAATTGAATA GAACTGAATA TAAATGGGTC AAAACCACAA TTCTAGGATC CCGTGAACAC 
TTCTGCATCA ATCAAAAAGT CAAGAAAATC AAAGAATCCA ATCGACAGGC TCATGTTTGT 
CGTGGATTGG TCTCGAAGCG AGCATGTCAT TATTATAATA AATTTGACGC GTGCACCACA 
GACAAAATGA CAGAATTTTT GGATAAAGGC GAAGCAATGG ATATTGAAGA TTTCGTGAAA 
CTTGGAACTC AAAATTCATT ATGCCCATAT TTCATGTCTC GGCAACGTTC TGAAACTGCT 
GAACTCATTC TTCTTCCCTA CAACTACATT ATCGATCCAA AAATGAGACG AAGATACAAG 
CTGGACCTGA AAAATTCAAT TGTGATCTTC GATGAGGCTC ATAATCTAGA ATCAATTTGC 
GAATCCAATG CATCTGCTGA GCTCACTTCC ACGTCAATTG CTCTTTGTAT TGAAGAGCTG 
AAAAAGGTTC TGGCGTTGTT GGTAGATGAA GAAGAGACTG CAAGAAGTGA AGCAGATGCT 
GAAACTGGAG CATTCGGAAG TGCAAAAATT GATTTAACAA AGAAATTGAT TGAGAATTTG 
AGAACTGAAG ATTTGATGAC AGTTTTAGAG AAAATATTTT CATTGGAAGA AGAAATGGAT 
AAATTATTTG GATCATCTCA ATTAAAATCT GTACCACCAT TGAGTGGAAA AGCAAGTGAC 
GGTGAAATTC TTCTTGAAAC TCTTGCAAAA GCTGGATTCG ATGCAAATTC TGTTGAACGG 
CTTGTTGATG TTCTTCGAGA TGCCATTTCT TATCTTTTGT CTAAAAATGA AGAAGTCGCT 
CTAACCGAAA AAGGAGATGG AATGGAAAAA GTGGCGGATT TCTTGTTGTC CATCTATTCG 
ACACACGCTC AAGATGTTGC TGCTGCTGTT GGCGAGGAAA CTGTTAAACT AGTTGATCGA 
GTTGATCCAA AAACTGTGGC TAGAAATTGC AAATTGTATA TTCAAAAGGA TAAGGACAAT 
GAGAAGCTTA CAATCAAATA TTTCTGTTTC CAAGCTTCAA TCTCGATGAG AATGCTCAAA 
ATGCGAGGAG TTCGAAATGT TCTTCTGGCC AGTGGAACCC TTTCACCAAT ACAGGCGTTC 
ACTTATAATA TGGGATTGAA TTTCGGTGCA ATTCTTGAGA ATGAGCATGC TCTGAAGCAA 
GTTCCAGTTT TAACTTCAAT TGTGACACGT GGAAAACGAG GAGGACTCGC CGGTTCATTC 
CAAAATCGAA AAAATCTGGA TTACGTGACA GGTGTAGCAG AAGCATTGTT ACGTGTCATG 
GAAGTTATTC CGCAAGGAAT TCTCATTTTC TTCTCATCAT ATTCTCAAAT GGATGAACTT 
GTTGCGACGT GGAAAACGAC GAAATGGTCG AGTAATTCGA ATGAATCGTT TTGGGAAAAA 
ATGGAGAAGA CGAAGAGAGT TGTGGTAGAA CCAAGAGCTA AAGAAGAACT AGCTGCTATT 
CGTCTGAGAT ATACACAAGG CGTCTCTGAA CAACACGGCG CTGCTCTATT AGCAGTTTGT 
CGTGGAAAAG TATCAGAAGG AATCGATTTT TGTGATGCCG AATCAAGAGC CGTCATTATT 
ATTGGAATCC CATATCCACC GATTCATGAT GAGAGAGTTG TTTTGAAGAA AATGTATTTG 
GACGATCTGA TGGGCAGAAA AGACACGAAA TCTGAACGTC AATCGTCTCA AGATTGGTAT 
CAAATGGAAG CGTTTAGAGC TGTAAATCAA GCAATCGGCC GAGTTCTTCG TCATAAGGAT 
GATTTTGGAA CTGTTGTTCT GATGGATACA CGTTATGCTT CAGCAAAACC TGAAATGTTT 
CCGAAATGGC TCAGAAATAC GATTTCTCGG AGTGACACTG ATGGGTGCGC TCTCAAGACA 
TCACGATTTT TCAAAGAAAG AGGACATTTG ATTGAGAATT CGAAGACCGA ATACATAAAA 
AAGCAGGCAA AACAGTGCAA ATCGTTCCGA CAAGTGAAGC AAACAGCTGC ATCAGATTCC 
AAAGATGATA TTATTGAAAT CACATTAGAA GATATGTTCT CGCCTGCAAA TATGAAATTG 
GAAAAGAAAG AAATCACTAA ATTACGTCCT CCACAATTGA TACCTTCAAC TTCTTCAGTT 
TTCAGCCTAC CAACGAACGA AGATGAGCTG AAAATCAAAA AATGGGAGCA AGAAAACGAT 
ATTCAATGCT TATCTTCAAT ACCCCTTGAA TCTAATAAAC GAAAGTTTAA AATTGAAACA 
CCAGGGCCAT CGACATCTAC GCTGACACAG AAAAGTGAAC CACCAAAAAA GAAGAAAATT 
CTGCTTTTGA CACGAAATAC TCTACCTGAT GAATATCAAA AAGCAATTGA AATTCCAACT 
TCTGAACTTT TGAAAGATAT GTCTGACGAC AATAAAAAGC AATTTGCCAC CACACTCAGA 
AGCTACAAAG CTGAAAGTAT CAGATGGGAT GAAGTTTTTC AAAGATTTCG GCCAATTTTT 
GTTCCGCACA AGGCTGATTT GTTTATTGCT TGCTCCAACG TTCTCCGATC GGAAGACAAA 
ATGAAATATC TGAAAAAGGC GCTTGAATCG AAGATCCACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rtel-1 ( NM_060368.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492769.1 Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), partial mRNA.

Price & Availability↑

CloneID	OCa335668
Clone ID Related Accession (Same CDS sequence)	NM_060368.5 , NM_060368.4
Accession Version	NM_060368.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Regulator of telomere elongation helicase 1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060368.3. COMPLETENESS: incomplete on both ends.

ATGCCGCCGA AAGCTTCAAA TTCAACGGAA GTTTGGATTA ACCCGAAGTT AAGTGTAAAA 
TTCCCATTTG AACCATACGA ATGTCAGAGG ATCTTCATGA AGAACGTTGT CGATGTACTG 
GATAGAAAAC TAGACGCAGC ACTTGAAAGT CCCACGGGTA CCGGAAAAAC GCTGTCTCTT 
CTCTGTTCGA CGCTCGCATG GGTTCAACGT CAAAAAGAGA CGAAGCCGCT GGATTTTGCC 
ACGTGGCAGA CGTCAGGAGC CGGTGGAGCA GAAAAAACTG ATGAGAAATT GAAGAGTGCA 
TACGTTCCGA CGATATTTTA TGCATCAAGA ACACATTCAC AGTTGGAACA AGTTGTTCAT 
GAATTGAATA GAACTGAATA TAAATGGGTC AAAACCACAA TTCTAGGATC CCGTGAACAC 
TTCTGCATCA ATCAAAAAGT CAAGAAAATC AAAGAATCCA ATCGACAGGC TCATGTTTGT 
CGTGGATTGG TCTCGAAGCG AGCATGTCAT TATTATAATA AATTTGACGC GTGCACCACA 
GACAAAATGA CAGAATTTTT GGATAAAGGC GAAGCAATGG ATATTGAAGA TTTCGTGAAA 
CTTGGAACTC AAAATTCATT ATGCCCATAT TTCATGTCTC GGCAACGTTC TGAAACTGCT 
GAACTCATTC TTCTTCCCTA CAACTACATT ATCGATCCAA AAATGAGACG AAGATACAAG 
CTGGACCTGA AAAATTCAAT TGTGATCTTC GATGAGGCTC ATAATCTAGA ATCAATTTGC 
GAATCCAATG CATCTGCTGA GCTCACTTCC ACGTCAATTG CTCTTTGTAT TGAAGAGCTG 
AAAAAGGTTC TGGCGTTGTT GGTAGATGAA GAAGAGACTG CAAGAAGTGA AGCAGATGCT 
GAAACTGGAG CATTCGGAAG TGCAAAAATT GATTTAACAA AGAAATTGAT TGAGAATTTG 
AGAACTGAAG ATTTGATGAC AGTTTTAGAG AAAATATTTT CATTGGAAGA AGAAATGGAT 
AAATTATTTG GATCATCTCA ATTAAAATCT GTACCACCAT TGAGTGGAAA AGCAAGTGAC 
GGTGAAATTC TTCTTGAAAC TCTTGCAAAA GCTGGATTCG ATGCAAATTC TGTTGAACGG 
CTTGTTGATG TTCTTCGAGA TGCCATTTCT TATCTTTTGT CTAAAAATGA AGAAGTCGCT 
CTAACCGAAA AAGGAGATGG AATGGAAAAA GTGGCGGATT TCTTGTTGTC CATCTATTCG 
ACACACGCTC AAGATGTTGC TGCTGCTGTT GGCGAGGAAA CTGTTAAACT AGTTGATCGA 
GTTGATCCAA AAACTGTGGC TAGAAATTGC AAATTGTATA TTCAAAAGGA TAAGGACAAT 
GAGAAGCTTA CAATCAAATA TTTCTGTTTC CAAGCTTCAA TCTCGATGAG AATGCTCAAA 
ATGCGAGGAG TTCGAAATGT TCTTCTGGCC AGTGGAACCC TTTCACCAAT ACAGGCGTTC 
ACTTATAATA TGGGATTGAA TTTCGGTGCA ATTCTTGAGA ATGAGCATGC TCTGAAGCAA 
GTTCCAGTTT TAACTTCAAT TGTGACACGT GGAAAACGAG GAGGACTCGC CGGTTCATTC 
CAAAATCGAA AAAATCTGGA TTACGTGACA GGTGTAGCAG AAGCATTGTT ACGTGTCATG 
GAAGTTATTC CGCAAGGAAT TCTCATTTTC TTCTCATCAT ATTCTCAAAT GGATGAACTT 
GTTGCGACGT GGAAAACGAC GAAATGGTCG AGTAATTCGA ATGAATCGTT TTGGGAAAAA 
ATGGAGAAGA CGAAGAGAGT TGTGGTAGAA CCAAGAGCTA AAGAAGAACT AGCTGCTATT 
CGTCTGAGAT ATACACAAGG CGTCTCTGAA CAACACGGCG CTGCTCTATT AGCAGTTTGT 
CGTGGAAAAG TATCAGAAGG AATCGATTTT TGTGATGCCG AATCAAGAGC CGTCATTATT 
ATTGGAATCC CATATCCACC GATTCATGAT GAGAGAGTTG TTTTGAAGAA AATGTATTTG 
GACGATCTGA TGGGCAGAAA AGACACGAAA TCTGAACGTC AATCGTCTCA AGATTGGTAT 
CAAATGGAAG CGTTTAGAGC TGTAAATCAA GCAATCGGCC GAGTTCTTCG TCATAAGGAT 
GATTTTGGAA CTGTTGTTCT GATGGATACA CGTTATGCTT CAGCAAAACC TGAAATGTTT 
CCGAAATGGC TCAGAAATAC GATTTCTCGG AGTGACACTG ATGGGTGCGC TCTCAAGACA 
TCACGATTTT TCAAAGAAAG AGGACATTTG ATTGAGAATT CGAAGACCGA ATACATAAAA 
AAGCAGGCAA AACAGTGCAA ATCGTTCCGA CAAGTGAAGC AAACAGCTGC ATCAGATTCC 
AAAGATGATA TTATTGAAAT CACATTAGAA GATATGTTCT CGCCTGCAAA TATGAAATTG 
GAAAAGAAAG AAATCACTAA ATTACGTCCT CCACAATTGA TACCTTCAAC TTCTTCAGTT 
TTCAGCCTAC CAACGAACGA AGATGAGCTG AAAATCAAAA AATGGGAGCA AGAAAACGAT 
ATTCAATGCT TATCTTCAAT ACCCCTTGAA TCTAATAAAC GAAAGTTTAA AATTGAAACA 
CCAGGGCCAT CGACATCTAC GCTGACACAG AAAAGTGAAC CACCAAAAAA GAAGAAAATT 
CTGCTTTTGA CACGAAATAC TCTACCTGAT GAATATCAAA AAGCAATTGA AATTCCAACT 
TCTGAACTTT TGAAAGATAT GTCTGACGAC AATAAAAAGC AATTTGCCAC CACACTCAGA 
AGCTACAAAG CTGAAAGTAT CAGATGGGAT GAAGTTTTTC AAAGATTTCG GCCAATTTTT 
GTTCCGCACA AGGCTGATTT GTTTATTGCT TGCTCCAACG TTCTCCGATC GGAAGACAAA 
ATGAAATATC TGAAAAAGGC GCTTGAATCG AAGATCCACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492769.1
CDS	1..2985
Translation	MPPKASNSTEVWINPKLSVKFPFEPYECQRIFMKNVVDVLDRKLDAALESPTGTGKTLSLLCSTLAWVQRQKETKPLDFATWQTSGAGGAEKTDEKLKSAYVPTIFYASRTHSQLEQVVHELNRTEYKWVKTTILGSREHFCINQKVKKIKESNRQAHVCRGLVSKRACHYYNKFDACTTDKMTEFLDKGEAMDIEDFVKLGTQNSLCPYFMSRQRSETAELILLPYNYIIDPKMRRRYKLDLKNSIVIFDEAHNLESICESNASAELTSTSIALCIEELKKVLALLVDEEETARSEADAETGAFGSAKIDLTKKLIENLRTEDLMTVLEKIFSLEEEMDKLFGSSQLKSVPPLSGKASDGEILLETLAKAGFDANSVERLVDVLRDAISYLLSKNEEVALTEKGDGMEKVADFLLSIYSTHAQDVAAAVGEETVKLVDRVDPKTVARNCKLYIQKDKDNEKLTIKYFCFQASISMRMLKMRGVRNVLLASGTLSPIQAFTYNMGLNFGAILENEHALKQVPVLTSIVTRGKRGGLAGSFQNRKNLDYVTGVAEALLRVMEVIPQGILIFFSSYSQMDELVATWKTTKWSSNSNESFWEKMEKTKRVVVEPRAKEELAAIRLRYTQGVSEQHGAALLAVCRGKVSEGIDFCDAESRAVIIIGIPYPPIHDERVVLKKMYLDDLMGRKDTKSERQSSQDWYQMEAFRAVNQAIGRVLRHKDDFGTVVLMDTRYASAKPEMFPKWLRNTISRSDTDGCALKTSRFFKERGHLIENSKTEYIKKQAKQCKSFRQVKQTAASDSKDDIIEITLEDMFSPANMKLEKKEITKLRPPQLIPSTSSVFSLPTNEDELKIKKWEQENDIQCLSSIPLESNKRKFKIETPGPSTSTLTQKSEPPKKKKILLLTRNTLPDEYQKAIEIPTSELLKDMSDDNKKQFATTLRSYKAESIRWDEVFQRFRPIFVPHKADLFIACSNVLRSEDKMKYLKKALESKIHT

Target ORF information:

RefSeq Version	NM_060368.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Regulator of telomere elongation helicase 1 homolog (rtel-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060368.4

ORF Insert Sequence:

ATGCCGCCGA AAGCTTCAAA TTCAACGGAA GTTTGGATTA ACCCGAAGTT AAGTGTAAAA 
TTCCCATTTG AACCATACGA ATGTCAGAGG ATCTTCATGA AGAACGTTGT CGATGTACTG 
GATAGAAAAC TAGACGCAGC ACTTGAAAGT CCCACGGGTA CCGGAAAAAC GCTGTCTCTT 
CTCTGTTCGA CGCTCGCATG GGTTCAACGT CAAAAAGAGA CGAAGCCGCT GGATTTTGCC 
ACGTGGCAGA CGTCAGGAGC CGGTGGAGCA GAAAAAACTG ATGAGAAATT GAAGAGTGCA 
TACGTTCCGA CGATATTTTA TGCATCAAGA ACACATTCAC AGTTGGAACA AGTTGTTCAT 
GAATTGAATA GAACTGAATA TAAATGGGTC AAAACCACAA TTCTAGGATC CCGTGAACAC 
TTCTGCATCA ATCAAAAAGT CAAGAAAATC AAAGAATCCA ATCGACAGGC TCATGTTTGT 
CGTGGATTGG TCTCGAAGCG AGCATGTCAT TATTATAATA AATTTGACGC GTGCACCACA 
GACAAAATGA CAGAATTTTT GGATAAAGGC GAAGCAATGG ATATTGAAGA TTTCGTGAAA 
CTTGGAACTC AAAATTCATT ATGCCCATAT TTCATGTCTC GGCAACGTTC TGAAACTGCT 
GAACTCATTC TTCTTCCCTA CAACTACATT ATCGATCCAA AAATGAGACG AAGATACAAG 
CTGGACCTGA AAAATTCAAT TGTGATCTTC GATGAGGCTC ATAATCTAGA ATCAATTTGC 
GAATCCAATG CATCTGCTGA GCTCACTTCC ACGTCAATTG CTCTTTGTAT TGAAGAGCTG 
AAAAAGGTTC TGGCGTTGTT GGTAGATGAA GAAGAGACTG CAAGAAGTGA AGCAGATGCT 
GAAACTGGAG CATTCGGAAG TGCAAAAATT GATTTAACAA AGAAATTGAT TGAGAATTTG 
AGAACTGAAG ATTTGATGAC AGTTTTAGAG AAAATATTTT CATTGGAAGA AGAAATGGAT 
AAATTATTTG GATCATCTCA ATTAAAATCT GTACCACCAT TGAGTGGAAA AGCAAGTGAC 
GGTGAAATTC TTCTTGAAAC TCTTGCAAAA GCTGGATTCG ATGCAAATTC TGTTGAACGG 
CTTGTTGATG TTCTTCGAGA TGCCATTTCT TATCTTTTGT CTAAAAATGA AGAAGTCGCT 
CTAACCGAAA AAGGAGATGG AATGGAAAAA GTGGCGGATT TCTTGTTGTC CATCTATTCG 
ACACACGCTC AAGATGTTGC TGCTGCTGTT GGCGAGGAAA CTGTTAAACT AGTTGATCGA 
GTTGATCCAA AAACTGTGGC TAGAAATTGC AAATTGTATA TTCAAAAGGA TAAGGACAAT 
GAGAAGCTTA CAATCAAATA TTTCTGTTTC CAAGCTTCAA TCTCGATGAG AATGCTCAAA 
ATGCGAGGAG TTCGAAATGT TCTTCTGGCC AGTGGAACCC TTTCACCAAT ACAGGCGTTC 
ACTTATAATA TGGGATTGAA TTTCGGTGCA ATTCTTGAGA ATGAGCATGC TCTGAAGCAA 
GTTCCAGTTT TAACTTCAAT TGTGACACGT GGAAAACGAG GAGGACTCGC CGGTTCATTC 
CAAAATCGAA AAAATCTGGA TTACGTGACA GGTGTAGCAG AAGCATTGTT ACGTGTCATG 
GAAGTTATTC CGCAAGGAAT TCTCATTTTC TTCTCATCAT ATTCTCAAAT GGATGAACTT 
GTTGCGACGT GGAAAACGAC GAAATGGTCG AGTAATTCGA ATGAATCGTT TTGGGAAAAA 
ATGGAGAAGA CGAAGAGAGT TGTGGTAGAA CCAAGAGCTA AAGAAGAACT AGCTGCTATT 
CGTCTGAGAT ATACACAAGG CGTCTCTGAA CAACACGGCG CTGCTCTATT AGCAGTTTGT 
CGTGGAAAAG TATCAGAAGG AATCGATTTT TGTGATGCCG AATCAAGAGC CGTCATTATT 
ATTGGAATCC CATATCCACC GATTCATGAT GAGAGAGTTG TTTTGAAGAA AATGTATTTG 
GACGATCTGA TGGGCAGAAA AGACACGAAA TCTGAACGTC AATCGTCTCA AGATTGGTAT 
CAAATGGAAG CGTTTAGAGC TGTAAATCAA GCAATCGGCC GAGTTCTTCG TCATAAGGAT 
GATTTTGGAA CTGTTGTTCT GATGGATACA CGTTATGCTT CAGCAAAACC TGAAATGTTT 
CCGAAATGGC TCAGAAATAC GATTTCTCGG AGTGACACTG ATGGGTGCGC TCTCAAGACA 
TCACGATTTT TCAAAGAAAG AGGACATTTG ATTGAGAATT CGAAGACCGA ATACATAAAA 
AAGCAGGCAA AACAGTGCAA ATCGTTCCGA CAAGTGAAGC AAACAGCTGC ATCAGATTCC 
AAAGATGATA TTATTGAAAT CACATTAGAA GATATGTTCT CGCCTGCAAA TATGAAATTG 
GAAAAGAAAG AAATCACTAA ATTACGTCCT CCACAATTGA TACCTTCAAC TTCTTCAGTT 
TTCAGCCTAC CAACGAACGA AGATGAGCTG AAAATCAAAA AATGGGAGCA AGAAAACGAT 
ATTCAATGCT TATCTTCAAT ACCCCTTGAA TCTAATAAAC GAAAGTTTAA AATTGAAACA 
CCAGGGCCAT CGACATCTAC GCTGACACAG AAAAGTGAAC CACCAAAAAA GAAGAAAATT 
CTGCTTTTGA CACGAAATAC TCTACCTGAT GAATATCAAA AAGCAATTGA AATTCCAACT 
TCTGAACTTT TGAAAGATAT GTCTGACGAC AATAAAAAGC AATTTGCCAC CACACTCAGA 
AGCTACAAAG CTGAAAGTAT CAGATGGGAT GAAGTTTTTC AAAGATTTCG GCCAATTTTT 
GTTCCGCACA AGGCTGATTT GTTTATTGCT TGCTCCAACG TTCTCCGATC GGAAGACAAA 
ATGAAATATC TGAAAAAGGC GCTTGAATCG AAGATCCACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rtel-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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