ears-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ears-1
Entrez Gene ID	172904
Full Name	glutamyl(E) Amino-acyl tRNA Synthetase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060310.5	NP_492711.1	glutamyl(E) Amino-acyl tRNA Synthetase
NM_060310.6	NP_492711.1	glutamyl(E) Amino-acyl tRNA Synthetase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AEL021C	NP_984840.1
Drosophila melanogaster (fruit fly)	Aats-glupro	NP_524471.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002945	XP_311956.5
Caenorhabditis elegans (roundworm)	ears-1	NP_492711.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ears-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ears-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335643	NM_060310.6 Latest version!	Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa335643	NM_060310.5	Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ears-1 ( NM_060310.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492711.1 Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), mRNA.

Price & Availability↑

CloneID	OCa335643
Clone ID Related Accession (Same CDS sequence)	NM_060310.5 , NM_060310.6
Accession Version	NM_060310.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	glutamyl(E) Amino-acyl tRNA Synthetase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060310.5.

ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG 
CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA 
ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC 
GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC 
CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT 
TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT 
ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC 
AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA 
GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA 
GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT 
CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG 
AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT 
CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG 
AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA 
CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA 
GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG 
AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT 
GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC 
GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT 
CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA 
CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA 
GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA 
GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT 
GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG 
CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA 
ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA 
GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA 
GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG 
GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA 
ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA 
GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG 
AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC 
AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC 
CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC 
ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC 
GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG 
GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA 
ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG 
CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG 
GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA 
AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA 
CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT 
GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC 
CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA 
GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT 
GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC 
GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG 
AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG 
AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT 
CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG 
ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA 
ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT 
TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA 
GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC 
CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT 
CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT 
GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492711.1
CDS	19..3468
Translation	MMITRELVLKANREQPPYASILALAASGFSLEKSVQFSEKQQLALNLDGELLSNDVEIARIIAQSTDAADSLLGSSIIDFAVVDDLTNFIADAVTKNDYSLLLGKDFPTKILDNSLTVADFAIFSVAHNNPQLKAKFSGIIDKVLKEPTLAAAHNFVGLYKSAAAPTATASTGKEKKKDEGKFVELPGAEKGKVVVRFPPEASGYLHIGHAKAALLNQYYQQAFEGQLIMRFDDTNPAKENAHFEHVIKEDLSMLNIVPDRWTHSSDHFEMLLTMCEKLLKEGKAFVDDTDTETMRNEREQRQDSRNRSNTPEKNLQLWEEMKKGSPKGLTCCVRMKIDMKSNNGAMRDPTIYRCKPEEHVRTGLKYKVYPTYDFTCPIVDSVEGVTHALRTTEYHDRDDQYYFICDALGLRRPHIWEYARLNMTNTVMSKRKLTWFVDEGHVEGWDDPRLPTVRGVMRRGLTVEGLKQFIVAQGGSRSVVMMEWDKIWAFNKKVIDPVAPRYTALDSTSPLVSIELTDSISDDTSNVSLHPKNAEIGSKDVHKGKKLLLEQVDAAALKEGEIVTFVNWGNIKIGKIEKKGAVITKISATLQLDNTDYKKTTKVTWLGDVKAEAGKTIPVVTADYDHIISKAIIGKDEDWKQFINFDSVHYTKMVGEPAIKNVKKGDIIQIQRKGFYIVDQPYNPKSELSGVETPLLLIAIPDGHTGKEAEKPPKGAPVSAAASSGGSDALQLYNSIEQQGGIVRDLKTKDAKSQATKDAIAKLLDLKKKYKELTGSDHKPGAPPSAPAPTTPSSSESNSALNIYNLIEAQGLLVRELKGKDAKSQATKDAIAKLLELKKQYKEVSGSDHKPGVPPAASVASAPAPAPAAGSTNALDIYHQIESQGALVRELKGKDAKSQATKDAIAKLLALKKQYKEVTGSDHKPGVVPVSAPAPTPLAGGADIAGQIEAQGVLVRDLKNKDSKSQETKDAIAKLLELKKNYKEVTGSDYKPGPAPAAAPAKVTVPAPSVSGGDTLSKQIDDQALLVRELKMKDAKSQETKDAIAKLLQLKKQYKDATGSDYKPAPAQLAASAAAAPAPAPAFDEAALLKEIEEQGAVVRDAKSKDPKSQDSADAINKLLALKANFKKATGKDFPAPSNAQSKKGKKK

Target ORF information:

RefSeq Version	NM_060310.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060310.6

ORF Insert Sequence:

ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG 
CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA 
ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC 
GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC 
CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT 
TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT 
ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC 
AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA 
GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA 
GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT 
CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG 
AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT 
CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG 
AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA 
CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA 
GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG 
AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT 
GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC 
GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT 
CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA 
CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA 
GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA 
GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT 
GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG 
CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA 
ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA 
GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA 
GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG 
GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA 
ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA 
GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG 
AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC 
AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC 
CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC 
ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC 
GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG 
GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA 
ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG 
CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG 
GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA 
AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA 
CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT 
GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC 
CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA 
GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT 
GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC 
GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG 
AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG 
AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT 
CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG 
ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA 
ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT 
TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA 
GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC 
CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT 
CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT 
GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ears-1 ( NM_060310.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492711.1 Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), partial mRNA.

Price & Availability↑

CloneID	OCa335643
Clone ID Related Accession (Same CDS sequence)	NM_060310.5 , NM_060310.6
Accession Version	NM_060310.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	glutamyl(E) Amino-acyl tRNA Synthetase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060310.4. COMPLETENESS: incomplete on both ends.

ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG 
CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA 
ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC 
GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC 
CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT 
TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT 
ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC 
AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA 
GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA 
GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT 
CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG 
AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT 
CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG 
AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA 
CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA 
GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG 
AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT 
GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC 
GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT 
CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA 
CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA 
GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA 
GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT 
GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG 
CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA 
ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA 
GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA 
GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG 
GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA 
ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA 
GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG 
AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC 
AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC 
CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC 
ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC 
GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG 
GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA 
ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG 
CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG 
GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA 
AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA 
CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT 
GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC 
CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA 
GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT 
GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC 
GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG 
AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG 
AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT 
CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG 
ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA 
ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT 
TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA 
GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC 
CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT 
CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT 
GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492711.1
CDS	1..3450
Translation	MMITRELVLKANREQPPYASILALAASGFSLEKSVQFSEKQQLALNLDGELLSNDVEIARIIAQSTDAADSLLGSSIIDFAVVDDLTNFIADAVTKNDYSLLLGKDFPTKILDNSLTVADFAIFSVAHNNPQLKAKFSGIIDKVLKEPTLAAAHNFVGLYKSAAAPTATASTGKEKKKDEGKFVELPGAEKGKVVVRFPPEASGYLHIGHAKAALLNQYYQQAFEGQLIMRFDDTNPAKENAHFEHVIKEDLSMLNIVPDRWTHSSDHFEMLLTMCEKLLKEGKAFVDDTDTETMRNEREQRQDSRNRSNTPEKNLQLWEEMKKGSPKGLTCCVRMKIDMKSNNGAMRDPTIYRCKPEEHVRTGLKYKVYPTYDFTCPIVDSVEGVTHALRTTEYHDRDDQYYFICDALGLRRPHIWEYARLNMTNTVMSKRKLTWFVDEGHVEGWDDPRLPTVRGVMRRGLTVEGLKQFIVAQGGSRSVVMMEWDKIWAFNKKVIDPVAPRYTALDSTSPLVSIELTDSISDDTSNVSLHPKNAEIGSKDVHKGKKLLLEQVDAAALKEGEIVTFVNWGNIKIGKIEKKGAVITKISATLQLDNTDYKKTTKVTWLGDVKAEAGKTIPVVTADYDHIISKAIIGKDEDWKQFINFDSVHYTKMVGEPAIKNVKKGDIIQIQRKGFYIVDQPYNPKSELSGVETPLLLIAIPDGHTGKEAEKPPKGAPVSAAASSGGSDALQLYNSIEQQGGIVRDLKTKDAKSQATKDAIAKLLDLKKKYKELTGSDHKPGAPPSAPAPTTPSSSESNSALNIYNLIEAQGLLVRELKGKDAKSQATKDAIAKLLELKKQYKEVSGSDHKPGVPPAASVASAPAPAPAAGSTNALDIYHQIESQGALVRELKGKDAKSQATKDAIAKLLALKKQYKEVTGSDHKPGVVPVSAPAPTPLAGGADIAGQIEAQGVLVRDLKNKDSKSQETKDAIAKLLELKKNYKEVTGSDYKPGPAPAAAPAKVTVPAPSVSGGDTLSKQIDDQALLVRELKMKDAKSQETKDAIAKLLQLKKQYKDATGSDYKPAPAQLAASAAAAPAPAPAFDEAALLKEIEEQGAVVRDAKSKDPKSQDSADAINKLLALKANFKKATGKDFPAPSNAQSKKGKKK

Target ORF information:

RefSeq Version	NM_060310.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060310.5

ORF Insert Sequence:

ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG 
CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA 
ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC 
GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC 
CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT 
TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT 
ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC 
AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA 
GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA 
GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT 
CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG 
AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT 
CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG 
AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA 
CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA 
GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG 
AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT 
GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC 
GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT 
CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA 
CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA 
GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA 
GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT 
GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG 
CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA 
ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA 
GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA 
GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG 
GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA 
ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA 
GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG 
AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC 
AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC 
CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC 
ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC 
GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG 
GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA 
ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG 
CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG 
GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA 
AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA 
CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT 
GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC 
CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA 
GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT 
GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC 
GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG 
AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG 
AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT 
CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG 
ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA 
ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT 
TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA 
GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC 
CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT 
CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT 
GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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オンライン注文

ears-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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