ears-1 cDNA ORF clone, Caenorhabditis elegans

The following ears-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ears-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa335643 NM_060310.6
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Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
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OCa335643 NM_060310.5 Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa335643
    Clone ID Related Accession (Same CDS sequence) NM_060310.5 , NM_060310.6
    Accession Version NM_060310.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product glutamyl(E) Amino-acyl tRNA Synthetase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060310.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
    ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG
    CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA
    ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC
    GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC
    CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT
    TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT
    ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC
    AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA
    GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA
    GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT
    CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG
    AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT
    CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG
    AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA
    CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA
    GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG
    AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT
    GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC
    GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT
    CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA
    CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA
    GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA
    GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT
    GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG
    CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA
    ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA
    GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA
    GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG
    GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA
    ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA
    GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG
    AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC
    AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC
    CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC
    ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC
    GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG
    GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA
    ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG
    CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG
    GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA
    AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA
    CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT
    GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC
    CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA
    GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT
    GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC
    GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG
    AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG
    AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT
    CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG
    ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA
    ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT
    TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA
    GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC
    CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT
    CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT
    GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492711.1
    CDS19..3468
    Translation

    Target ORF information:

    RefSeq Version NM_060310.6
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_060310.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
    ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG
    CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA
    ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC
    GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC
    CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT
    TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT
    ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC
    AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA
    GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA
    GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT
    CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG
    AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT
    CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG
    AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA
    CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA
    GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG
    AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT
    GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC
    GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT
    CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA
    CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA
    GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA
    GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT
    GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG
    CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA
    ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA
    GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA
    GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG
    GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA
    ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA
    GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG
    AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC
    AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC
    CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC
    ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC
    GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG
    GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA
    ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG
    CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG
    GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA
    AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA
    CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT
    GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC
    CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA
    GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT
    GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC
    GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG
    AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG
    AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT
    CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG
    ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA
    ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT
    TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA
    GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC
    CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT
    CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT
    GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335643
    Clone ID Related Accession (Same CDS sequence) NM_060310.5 , NM_060310.6
    Accession Version NM_060310.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product glutamyl(E) Amino-acyl tRNA Synthetase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060310.4. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
    ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG
    CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA
    ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC
    GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC
    CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT
    TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT
    ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC
    AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA
    GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA
    GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT
    CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG
    AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT
    CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG
    AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA
    CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA
    GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG
    AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT
    GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC
    GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT
    CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA
    CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA
    GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA
    GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT
    GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG
    CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA
    ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA
    GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA
    GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG
    GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA
    ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA
    GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG
    AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC
    AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC
    CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC
    ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC
    GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG
    GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA
    ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG
    CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG
    GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA
    AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA
    CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT
    GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC
    CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA
    GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT
    GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC
    GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG
    AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG
    AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT
    CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG
    ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA
    ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT
    TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA
    GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC
    CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT
    CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT
    GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492711.1
    CDS1..3450
    Translation

    Target ORF information:

    RefSeq Version NM_060310.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans glutamyl(E) Amino-acyl tRNA Synthetase (ears-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_060310.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGATCA CCAGAGAGTT GGTGCTGAAA GCGAACCGAG AGCAACCGCC GTATGCGTCG 
    ATTTTGGCTC TTGCCGCGTC TGGATTTTCC CTCGAGAAGT CTGTTCAATT CTCTGAAAAG
    CAGCAGCTTG CTCTTAACTT AGACGGAGAA CTCCTGTCCA ATGACGTTGA AATTGCTAGA
    ATCATCGCTC AAAGCACTGA TGCTGCTGAT AGTCTTCTAG GATCTTCTAT CATCGATTTC
    GCTGTCGTTG ACGATCTTAC AAACTTCATT GCTGATGCTG TCACGAAGAA TGACTATTCC
    CTCCTTTTGG GAAAGGATTT TCCGACCAAA ATTCTCGATA ATTCCTTGAC TGTTGCTGAT
    TTTGCCATCT TCTCTGTGGC GCACAACAAT CCTCAATTGA AGGCCAAGTT CTCTGGAATT
    ATCGACAAAG TTCTCAAAGA ACCAACCCTG GCTGCTGCTC ACAACTTTGT CGGATTATAC
    AAAAGTGCAG CTGCTCCAAC TGCAACGGCA TCTACTGGAA AAGAAAAGAA GAAGGACGAA
    GGAAAGTTCG TTGAGCTTCC AGGAGCCGAG AAAGGAAAGG TTGTTGTCCG ATTCCCACCA
    GAGGCAAGTG GATATTTGCA TATTGGACAC GCTAAGGCAG CACTTCTCAA TCAATACTAT
    CAACAAGCTT TCGAAGGGCA ACTCATCATG CGATTCGATG ACACAAATCC AGCCAAAGAG
    AATGCTCATT TCGAACATGT TATCAAAGAG GACCTCTCAA TGCTCAACAT CGTTCCTGAT
    CGTTGGACTC ACTCTTCTGA TCATTTTGAA ATGCTTCTTA CCATGTGTGA GAAATTGTTG
    AAGGAAGGAA AGGCATTCGT CGATGATACT GACACTGAAA CTATGCGTAA TGAACGAGAA
    CAGAGACAAG ACAGTCGTAA TCGTTCAAAC ACTCCAGAAA AGAATCTCCA ATTGTGGGAA
    GAAATGAAAA AAGGTTCTCC AAAAGGATTA ACTTGCTGTG TTCGTATGAA GATCGACATG
    AAATCAAATA ATGGAGCCAT GAGAGATCCA ACTATCTACC GATGCAAGCC AGAGGAGCAT
    GTGAGAACAG GATTAAAGTA CAAGGTCTAT CCAACTTATG ACTTCACTTG TCCAATTGTC
    GATAGTGTAG AAGGTGTAAC TCATGCTCTC AGAACCACTG AATACCATGA TCGTGATGAT
    CAGTACTACT TCATCTGCGA TGCTCTCGGT CTTCGTCGTC CACACATTTG GGAATATGCA
    CGTCTAAACA TGACGAACAC AGTTATGAGC AAGCGTAAGC TCACCTGGTT TGTCGATGAA
    GGACATGTCG AGGGATGGGA TGATCCAAGA CTTCCAACTG TTCGAGGAGT TATGAGAAGA
    GGATTGACTG TCGAAGGATT GAAACAATTT ATTGTTGCAC AAGGAGGATC GAGATCAGTT
    GTCATGATGG AATGGGATAA GATTTGGGCG TTCAATAAGA AGGTTATTGA TCCAGTTGCG
    CCAAGATACA CAGCTCTCGA CTCCACATCT CCGCTTGTTT CCATCGAATT AACAGATTCA
    ATCTCTGACG ACACCTCCAA TGTGTCTCTT CATCCAAAGA ATGCTGAAAT TGGAAGCAAA
    GACGTTCACA AGGGCAAAAA ACTTTTGCTC GAACAAGTCG ATGCTGCAGC TCTGAAGGAA
    GGAGAAATTG TCACATTCGT CAACTGGGGT AACATAAAGA TTGGAAAAAT CGAGAAGAAG
    GGCGCCGTAA TAACGAAAAT CAGTGCAACT CTTCAGTTGG ATAATACTGA TTACAAGAAA
    ACTACAAAAG TGACATGGCT GGGAGATGTC AAGGCTGAGG CTGGAAAAAC TATTCCAGTA
    GTGACTGCTG ACTATGATCA TATTATCAGC AAAGCTATTA TTGGAAAGGA TGAAGATTGG
    AAGCAATTCA TCAATTTTGA CTCTGTGCAC TACACAAAGA TGGTTGGAGA ACCAGCAATC
    AAGAATGTGA AGAAGGGTGA TATCATTCAA ATTCAACGCA AAGGATTCTA CATTGTTGAC
    CAACCGTACA ATCCAAAGAG TGAACTCAGC GGAGTTGAAA CTCCTCTGCT TCTCATTGCC
    ATTCCTGATG GACATACTGG CAAAGAAGCT GAGAAGCCAC CGAAGGGAGC TCCAGTTAGC
    GCTGCTGCTT CTTCCGGAGG TTCCGATGCT CTCCAATTGT ACAATTCCAT TGAACAACAG
    GGTGGAATTG TCAGAGATTT GAAAACAAAA GATGCTAAAT CTCAAGCTAC AAAGGATGCA
    ATTGCTAAGC TTCTTGATTT GAAAAAGAAG TATAAGGAAT TGACCGGCTC TGATCACAAG
    CCAGGAGCTC CACCATCCGC TCCAGCTCCT ACTACTCCAT CGTCTTCTGA ATCGAACTCG
    GCTTTGAACA TTTACAATCT GATTGAAGCT CAAGGTCTAT TGGTTAGAGA ACTCAAAGGA
    AAAGATGCCA AGTCTCAAGC AACAAAAGAT GCTATCGCGA AGCTTCTTGA ATTGAAGAAA
    CAATACAAAG AGGTCAGTGG ATCCGATCAC AAGCCAGGTG TACCACCTGC TGCATCTGTT
    GCATCAGCTC CAGCACCAGC ACCTGCTGCA GGATCGACCA ACGCCTTGGA TATCTACCAC
    CAAATCGAAT CCCAAGGAGC TTTGGTTAGA GAACTCAAAG GAAAAGATGC GAAGTCTCAA
    GCAACAAAAG ATGCTATCGC TAAGCTTCTT GCTCTGAAGA AACAATACAA AGAGGTGACT
    GGTTCCGATC ATAAGCCAGG TGTTGTTCCA GTCTCTGCAC CAGCTCCAAC CCCTCTTGCC
    GGAGGAGCCG ATATTGCAGG TCAAATTGAA GCACAAGGAG TTCTTGTCAG AGATTTGAAG
    AACAAGGATT CCAAATCTCA AGAAACAAAG GATGCTATTG CGAAACTCCT AGAGCTCAAG
    AAGAACTATA AAGAGGTTAC TGGTTCCGAT TACAAGCCAG GCCCTGCTCC AGCAGCTGCT
    CCCGCGAAGG TTACGGTACC AGCTCCATCG GTCTCCGGGG GGGATACACT TTCCAAGCAG
    ATTGATGATC AAGCACTTCT TGTGAGAGAG TTGAAGATGA AAGACGCTAA ATCTCAAGAA
    ACCAAGGACG CCATCGCAAA ACTGTTGCAG TTGAAGAAAC AGTACAAGGA TGCGACCGGT
    TCGGATTATA AGCCAGCTCC AGCTCAATTG GCAGCTTCAG CCGCCGCCGC ACCAGCTCCA
    GCTCCAGCTT TCGACGAAGC TGCGCTTCTC AAGGAAATTG AAGAGCAAGG AGCAGTTGTC
    CGTGACGCAA AATCCAAAGA TCCAAAATCG CAAGATTCCG CCGATGCCAT TAACAAGCTT
    CTGGCTTTGA AGGCTAATTT CAAGAAAGCC ACTGGAAAAG ACTTCCCAGC TCCATCGAAT
    GCTCAATCGA AGAAAGGAAA GAAGAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.