ppfr-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ppfr-1
Entrez Gene ID	172761
Full Name	Protein Phosphatase Four Regulatory subunit
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001026217.3	NP_001021388.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001026219.3	NP_001021390.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001128984.2	NP_001122456.2	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001128984.3	NP_001122456.2	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001026217.4	NP_001021388.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001026218.4	NP_001021389.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001026219.4	NP_001021390.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1
NM_001026218.3	NP_001021389.1	Serine/threonine-protein phosphatase 4 regulatory subunit 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPFR-1, a regulatory subunit of a trimeric protein phosphatase 4 complex, enhanced katanin MT-severing activity during C. elegans meiosis.
Title: Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation.

The following ppfr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppfr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335884	NM_001026219.4 Latest version!	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa335885	NM_001026217.4 Latest version!	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa335886	NM_001026218.4 Latest version!	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa335394	NM_001128984.3 Latest version!	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa335394	NM_001128984.2	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa335885	NM_001026217.3	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa335884	NM_001026219.3	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa335886	NM_001026218.3	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppfr-1 ( NM_001026219.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021390.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Price & Availability↑

CloneID	OCa335884
Clone ID Related Accession (Same CDS sequence)	NM_001026219.3 , NM_001026219.4
Accession Version	NM_001026219.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026219.2. COMPLETENESS: incomplete on both ends.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG TTCCTGTCGC 
CAGAAAAGCG TGACGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021390.1
CDS	1..3141
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLSSCRQKSVTR

Target ORF information:

RefSeq Version	NM_001026219.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026219.3

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG TTCCTGTCGC 
CAGAAAAGCG TGACGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001026219.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021390.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

Price & Availability↑

CloneID	OCa335884
Clone ID Related Accession (Same CDS sequence)	NM_001026219.3 , NM_001026219.4
Accession Version	NM_001026219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026219.3.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG TTCCTGTCGC 
CAGAAAAGCG TGACGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021390.1
CDS	50..3190
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLSSCRQKSVTR

Target ORF information:

RefSeq Version	NM_001026219.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026219.4

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG TTCCTGTCGC 
CAGAAAAGCG TGACGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001026217.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021388.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

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CloneID	OCa335885
Clone ID Related Accession (Same CDS sequence)	NM_001026217.3 , NM_001026217.4
Accession Version	NM_001026217.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026217.2. COMPLETENESS: incomplete on both ends.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG AAACGATCAA 
AATCCAGACG TTCGCAATGG AATATTAATT CATTTGTATG ATTTCGTGAA GTTCCTGTCG 
CCAGAAAAGC GTGACGAGAT GATTCTTTCC CTTCCACAAT TCTTCCCAAT CGGAGCTCAA 
CCAGGAAATC AGGCACAGAA TGGTGATTGG AGATCTAGAT TTGAACTTAT ATCTCAACTT 
TCGAAGCTGT GCTCTCTGTA CAGTATTCAA GATGTCAATT TTCACATGTC TGGAATTGCT 
CTTACATTGG CTGATGATCG TGTCGCAGAA GTTCGACGTG AAGCTGTGAT GCTTGTTTCA 
ACGATCGTCG GAGCCCTTGT TACAGCTGAA TGGGATAACA TCAAAGGAGT TCGGGATATT 
GAAAATGGAC ATGTTGATGT GAAAAAGAAG AGTAACAAAA CTGATTTTTT GTCCGAACAA 
TTTGTCGATG ATATTGTATC CAGTTTTGCA AAAACTCAGA AATGGACAAG ACGTCAAACA 
TTCTGCTTCA TCTGCGCCCA AATTTTACGC GATAAGCAGT GCGACGGGAT TCAGTTCCGA 
TATTTCTTTG CTACTCCTCT TCAGCAGTTG GCTCAAGATA AAGTTCCAAA TGTCCGATTG 
GCATCTTGCG AGGCGTTAAG TGTGTGGAGA AAAACTTTGA TCGCTGCTCG CGCTCAGGAA 
AGAAGAGGTC AATCTCCGAT CTCTTCGCCA GCATCAGAAC CATCAGCTAT CCGTCGTGAC 
ATCAATCTTG TTTCCGATAT GATGGTCAAA CTCTCCAACG ATTCTGATAT TGATGCGGCA 
TTCATTGCAA AGAAGTGTCA AGGTTTAACA GATGATCATC AGCCAGTTGA TGTTGCTTCA 
CGAACTACAC GGATGCGTGA ACGAGAAGAA CAGTTCTTTG GAGATATTAT TCTTAGCTAT 
TCTCCTGGTT GTAATGCAAG AGTTTCTGGT AAAGAAATTA AACAGTTGAT CCGGCATGAT 
CAAGGAGCAG TTCAGAAAGC AGCTCCACTT CAATCAATGG AAGATTTTGG AGATGAGATA 
TTGGCTCATG CGGCAGATTC GGTGAATATT CAAACACCTC CGGAAGTAGT TGCTACTGTT 
CAACGAGTCT CAATTACAAA TGAACAATTT GAGGATAACA CTGAAGCGGT TGATATGGAA 
ATTGAAGAGG ATGAAGATGA AAACTCTACA GAGAAGAAGG AAAACACTGA AAAAACCGCA 
GAGGAAGAAT CTGAGAATGT TAAGAATACT GAGGATGAAC TGGATATACC TATGGATCCT 
GCACCTACTC TTCCACCACC AGTGACATCA AAAAATCAAT CTTCATCACC TATTACCGCC 
TCAAGTTCAG ATAACAACGA GACAAGTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021388.1
CDS	1..4473
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001026217.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026217.3

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG AAACGATCAA 
AATCCAGACG TTCGCAATGG AATATTAATT CATTTGTATG ATTTCGTGAA GTTCCTGTCG 
CCAGAAAAGC GTGACGAGAT GATTCTTTCC CTTCCACAAT TCTTCCCAAT CGGAGCTCAA 
CCAGGAAATC AGGCACAGAA TGGTGATTGG AGATCTAGAT TTGAACTTAT ATCTCAACTT 
TCGAAGCTGT GCTCTCTGTA CAGTATTCAA GATGTCAATT TTCACATGTC TGGAATTGCT 
CTTACATTGG CTGATGATCG TGTCGCAGAA GTTCGACGTG AAGCTGTGAT GCTTGTTTCA 
ACGATCGTCG GAGCCCTTGT TACAGCTGAA TGGGATAACA TCAAAGGAGT TCGGGATATT 
GAAAATGGAC ATGTTGATGT GAAAAAGAAG AGTAACAAAA CTGATTTTTT GTCCGAACAA 
TTTGTCGATG ATATTGTATC CAGTTTTGCA AAAACTCAGA AATGGACAAG ACGTCAAACA 
TTCTGCTTCA TCTGCGCCCA AATTTTACGC GATAAGCAGT GCGACGGGAT TCAGTTCCGA 
TATTTCTTTG CTACTCCTCT TCAGCAGTTG GCTCAAGATA AAGTTCCAAA TGTCCGATTG 
GCATCTTGCG AGGCGTTAAG TGTGTGGAGA AAAACTTTGA TCGCTGCTCG CGCTCAGGAA 
AGAAGAGGTC AATCTCCGAT CTCTTCGCCA GCATCAGAAC CATCAGCTAT CCGTCGTGAC 
ATCAATCTTG TTTCCGATAT GATGGTCAAA CTCTCCAACG ATTCTGATAT TGATGCGGCA 
TTCATTGCAA AGAAGTGTCA AGGTTTAACA GATGATCATC AGCCAGTTGA TGTTGCTTCA 
CGAACTACAC GGATGCGTGA ACGAGAAGAA CAGTTCTTTG GAGATATTAT TCTTAGCTAT 
TCTCCTGGTT GTAATGCAAG AGTTTCTGGT AAAGAAATTA AACAGTTGAT CCGGCATGAT 
CAAGGAGCAG TTCAGAAAGC AGCTCCACTT CAATCAATGG AAGATTTTGG AGATGAGATA 
TTGGCTCATG CGGCAGATTC GGTGAATATT CAAACACCTC CGGAAGTAGT TGCTACTGTT 
CAACGAGTCT CAATTACAAA TGAACAATTT GAGGATAACA CTGAAGCGGT TGATATGGAA 
ATTGAAGAGG ATGAAGATGA AAACTCTACA GAGAAGAAGG AAAACACTGA AAAAACCGCA 
GAGGAAGAAT CTGAGAATGT TAAGAATACT GAGGATGAAC TGGATATACC TATGGATCCT 
GCACCTACTC TTCCACCACC AGTGACATCA AAAAATCAAT CTTCATCACC TATTACCGCC 
TCAAGTTCAG ATAACAACGA GACAAGTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001026217.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021388.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

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CloneID	OCa335885
Clone ID Related Accession (Same CDS sequence)	NM_001026217.3 , NM_001026217.4
Accession Version	NM_001026217.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026217.3.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG AAACGATCAA 
AATCCAGACG TTCGCAATGG AATATTAATT CATTTGTATG ATTTCGTGAA GTTCCTGTCG 
CCAGAAAAGC GTGACGAGAT GATTCTTTCC CTTCCACAAT TCTTCCCAAT CGGAGCTCAA 
CCAGGAAATC AGGCACAGAA TGGTGATTGG AGATCTAGAT TTGAACTTAT ATCTCAACTT 
TCGAAGCTGT GCTCTCTGTA CAGTATTCAA GATGTCAATT TTCACATGTC TGGAATTGCT 
CTTACATTGG CTGATGATCG TGTCGCAGAA GTTCGACGTG AAGCTGTGAT GCTTGTTTCA 
ACGATCGTCG GAGCCCTTGT TACAGCTGAA TGGGATAACA TCAAAGGAGT TCGGGATATT 
GAAAATGGAC ATGTTGATGT GAAAAAGAAG AGTAACAAAA CTGATTTTTT GTCCGAACAA 
TTTGTCGATG ATATTGTATC CAGTTTTGCA AAAACTCAGA AATGGACAAG ACGTCAAACA 
TTCTGCTTCA TCTGCGCCCA AATTTTACGC GATAAGCAGT GCGACGGGAT TCAGTTCCGA 
TATTTCTTTG CTACTCCTCT TCAGCAGTTG GCTCAAGATA AAGTTCCAAA TGTCCGATTG 
GCATCTTGCG AGGCGTTAAG TGTGTGGAGA AAAACTTTGA TCGCTGCTCG CGCTCAGGAA 
AGAAGAGGTC AATCTCCGAT CTCTTCGCCA GCATCAGAAC CATCAGCTAT CCGTCGTGAC 
ATCAATCTTG TTTCCGATAT GATGGTCAAA CTCTCCAACG ATTCTGATAT TGATGCGGCA 
TTCATTGCAA AGAAGTGTCA AGGTTTAACA GATGATCATC AGCCAGTTGA TGTTGCTTCA 
CGAACTACAC GGATGCGTGA ACGAGAAGAA CAGTTCTTTG GAGATATTAT TCTTAGCTAT 
TCTCCTGGTT GTAATGCAAG AGTTTCTGGT AAAGAAATTA AACAGTTGAT CCGGCATGAT 
CAAGGAGCAG TTCAGAAAGC AGCTCCACTT CAATCAATGG AAGATTTTGG AGATGAGATA 
TTGGCTCATG CGGCAGATTC GGTGAATATT CAAACACCTC CGGAAGTAGT TGCTACTGTT 
CAACGAGTCT CAATTACAAA TGAACAATTT GAGGATAACA CTGAAGCGGT TGATATGGAA 
ATTGAAGAGG ATGAAGATGA AAACTCTACA GAGAAGAAGG AAAACACTGA AAAAACCGCA 
GAGGAAGAAT CTGAGAATGT TAAGAATACT GAGGATGAAC TGGATATACC TATGGATCCT 
GCACCTACTC TTCCACCACC AGTGACATCA AAAAATCAAT CTTCATCACC TATTACCGCC 
TCAAGTTCAG ATAACAACGA GACAAGTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021388.1
CDS	50..4522
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001026217.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026217.4

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTCACCAGAT 
GCAGCTGAAG ATTCCGATGA TTTCACATCG GAAACACGGA CGACAAGTCC ACGTGGTCGG 
CGTATTGCCA CCAACGCGTG GTCGAAGCTT GATATTCGGC AGAAGATATT TCTCCGGCGA 
CTTGGAGCTC TACCACCGAG CTTTCCGAAG AAAAAGGCGA CACACAGCAG CAGCAGCAGT 
CCGAGAAAAT CACCAACAAG ATCGCCACTG CTGTCGTCGC GAAAAAAGGG ACTTTTGTTT 
GATTTTGCGG CGCCTATTGA AATCAAATCA TCGTCTTCTG ATCATTCGGA GACAATTCCA 
GTCCGGACAG CCAATCAACA GCAAGAAAAA GCAGATGATG ATAAGCTGAA TACCGATGAA 
CGGCAGCAAT ACATGGAAAA CTCTGGTCCA AAGTTCAACA CTGATGAAGA TTCAACGAAT 
GATTCAGATA GTGACGATGA GGATAATGAT TTTGAAAAGT CTTTTGTTGC TGACGAAGAT 
GACGGAGAAC AAGACGATAG TCCATTATCA ATTGAAATTA AATCATCTTC TTCGTCAGAC 
TTTGAATCAG TTTCGGCAGC CACTCCAACA TCTCAGGAGC CAGTATCTAA TGAGTTTTCA 
TTGACTTATT GGTCGTCAAA CTATGCTATT TCGTCTATCG AAGATGAGCT CAAACCTTTT 
GGATCCTCAT CCTCATTTAC CAATTCTCCA ACTTCTAACA GTTATTTGGA GAGCCGATTC 
GATGTGATGA AGGTTAATCT TTCGCCAAGA GCTCGATCAG TGTCATTTGG AACTAATGGA 
TCTCCGATAT CCACTTCACC ACGAAGAAAA GCCAACAGTA CATCTGAACC ATCTCCACCA 
AACACCGGAA ACTTGAAAAG CGATGACTCC ATTGTAATGC TCAATACGGA AGAAAAGGAG 
AAACTGGGAA TTTTAGATTT CGATGAAATG AACATGTCAA TATCATCGAA TTCATCGATT 
CATGGAGAAG CCGCAATGAC GGAAGATTCG GATATTAGTC TAACTTCGAT TGAACAGGCA 
GCACTCCAAC ATGTTCCAGT AGATCTTGTT GAATCTTATA TGAGAGTCAT GGGGCCGATT 
GGAGGTAGTG GAAGCGGAGG TGGTGGTAAT GGAAGTTCTA CAACCATAGC TACTGGAGAT 
CCATCGCTTT GCGCGGAGAC CTATCGTCAT TGTGCTCACA ACTTTCCCGC AGTGTGCTAT 
ACGCTTGGAC GTGCTGCATG GCCTCGGCTT AAGCTTGTAT TCCGGAAGCT TGCGATGGAT 
GAGCAGGCTC GAGTTCGTCA GTCGATATCT CATTCGATTC ATGAAATAGC AAATATGCTT 
GGACAGGAGA TCACAGATGA AGATTTGTTG CCAGTTTTCT ATGATTTGAG AAACGATCAA 
AATCCAGACG TTCGCAATGG AATATTAATT CATTTGTATG ATTTCGTGAA GTTCCTGTCG 
CCAGAAAAGC GTGACGAGAT GATTCTTTCC CTTCCACAAT TCTTCCCAAT CGGAGCTCAA 
CCAGGAAATC AGGCACAGAA TGGTGATTGG AGATCTAGAT TTGAACTTAT ATCTCAACTT 
TCGAAGCTGT GCTCTCTGTA CAGTATTCAA GATGTCAATT TTCACATGTC TGGAATTGCT 
CTTACATTGG CTGATGATCG TGTCGCAGAA GTTCGACGTG AAGCTGTGAT GCTTGTTTCA 
ACGATCGTCG GAGCCCTTGT TACAGCTGAA TGGGATAACA TCAAAGGAGT TCGGGATATT 
GAAAATGGAC ATGTTGATGT GAAAAAGAAG AGTAACAAAA CTGATTTTTT GTCCGAACAA 
TTTGTCGATG ATATTGTATC CAGTTTTGCA AAAACTCAGA AATGGACAAG ACGTCAAACA 
TTCTGCTTCA TCTGCGCCCA AATTTTACGC GATAAGCAGT GCGACGGGAT TCAGTTCCGA 
TATTTCTTTG CTACTCCTCT TCAGCAGTTG GCTCAAGATA AAGTTCCAAA TGTCCGATTG 
GCATCTTGCG AGGCGTTAAG TGTGTGGAGA AAAACTTTGA TCGCTGCTCG CGCTCAGGAA 
AGAAGAGGTC AATCTCCGAT CTCTTCGCCA GCATCAGAAC CATCAGCTAT CCGTCGTGAC 
ATCAATCTTG TTTCCGATAT GATGGTCAAA CTCTCCAACG ATTCTGATAT TGATGCGGCA 
TTCATTGCAA AGAAGTGTCA AGGTTTAACA GATGATCATC AGCCAGTTGA TGTTGCTTCA 
CGAACTACAC GGATGCGTGA ACGAGAAGAA CAGTTCTTTG GAGATATTAT TCTTAGCTAT 
TCTCCTGGTT GTAATGCAAG AGTTTCTGGT AAAGAAATTA AACAGTTGAT CCGGCATGAT 
CAAGGAGCAG TTCAGAAAGC AGCTCCACTT CAATCAATGG AAGATTTTGG AGATGAGATA 
TTGGCTCATG CGGCAGATTC GGTGAATATT CAAACACCTC CGGAAGTAGT TGCTACTGTT 
CAACGAGTCT CAATTACAAA TGAACAATTT GAGGATAACA CTGAAGCGGT TGATATGGAA 
ATTGAAGAGG ATGAAGATGA AAACTCTACA GAGAAGAAGG AAAACACTGA AAAAACCGCA 
GAGGAAGAAT CTGAGAATGT TAAGAATACT GAGGATGAAC TGGATATACC TATGGATCCT 
GCACCTACTC TTCCACCACC AGTGACATCA AAAAATCAAT CTTCATCACC TATTACCGCC 
TCAAGTTCAG ATAACAACGA GACAAGTGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001026218.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021389.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

Price & Availability↑

CloneID	OCa335886
Clone ID Related Accession (Same CDS sequence)	NM_001026218.4 , NM_001026218.3
Accession Version	NM_001026218.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026218.3.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTACATGGAA 
AACTCTGGTC CAAAGTTCAA CACTGATGAA GATTCAACGA ATGATTCAGA TAGTGACGAT 
GAGGATAATG ATTTTGAAAA GTCTTTTGTT GCTGACGAAG ATGACGGAGA ACAAGACGAT 
AGTCCATTAT CAATTGAAAT TAAATCATCT TCTTCGTCAG ACTTTGAATC AGTTTCGGCA 
GCCACTCCAA CATCTCAGGA GCCAGTATCT AATGAGTTTT CATTGACTTA TTGGTCGTCA 
AACTATGCTA TTTCGTCTAT CGAAGATGAG CTCAAACCTT TTGGATCCTC ATCCTCATTT 
ACCAATTCTC CAACTTCTAA CAGTTATTTG GAGAGCCGAT TCGATGTGAT GAAGGTTAAT 
CTTTCGCCAA GAGCTCGATC AGTGTCATTT GGAACTAATG GATCTCCGAT ATCCACTTCA 
CCACGAAGAA AAGCCAACAG TACATCTGAA CCATCTCCAC CAAACACCGG AAACTTGAAA 
AGCGATGACT CCATTGTAAT GCTCAATACG GAAGAAAAGG AGAAACTGGG AATTTTAGAT 
TTCGATGAAA TGAACATGTC AATATCATCG AATTCATCGA TTCATGGAGA AGCCGCAATG 
ACGGAAGATT CGGATATTAG TCTAACTTCG ATTGAACAGG CAGCACTCCA ACATGTTCCA 
GTAGATCTTG TTGAATCTTA TATGAGAGTC ATGGGGCCGA TTGGAGGTAG TGGAAGCGGA 
GGTGGTGGTA ATGGAAGTTC TACAACCATA GCTACTGGAG ATCCATCGCT TTGCGCGGAG 
ACCTATCGTC ATTGTGCTCA CAACTTTCCC GCAGTGTGCT ATACGCTTGG ACGTGCTGCA 
TGGCCTCGGC TTAAGCTTGT ATTCCGGAAG CTTGCGATGG ATGAGCAGGC TCGAGTTCGT 
CAGTCGATAT CTCATTCGAT TCATGAAATA GCAAATATGC TTGGACAGGA GATCACAGAT 
GAAGATTTGT TGCCAGTTTT CTATGATTTG AGAAACGATC AAAATCCAGA CGTTCGCAAT 
GGAATATTAA TTCATTTGTA TGATTTCGTG AAGTTCCTGT CGCCAGAAAA GCGTGACGAG 
ATGATTCTTT CCCTTCCACA ATTCTTCCCA ATCGGAGCTC AACCAGGAAA TCAGGCACAG 
AATGGTGATT GGAGATCTAG ATTTGAACTT ATATCTCAAC TTTCGAAGCT GTGCTCTCTG 
TACAGTATTC AAGATGTCAA TTTTCACATG TCTGGAATTG CTCTTACATT GGCTGATGAT 
CGTGTCGCAG AAGTTCGACG TGAAGCTGTG ATGCTTGTTT CAACGATCGT CGGAGCCCTT 
GTTACAGCTG AATGGGATAA CATCAAAGGA GTTCGGGATA TTGAAAATGG ACATGTTGAT 
GTGAAAAAGA AGAGTAACAA AACTGATTTT TTGTCCGAAC AATTTGTCGA TGATATTGTA 
TCCAGTTTTG CAAAAACTCA GAAATGGACA AGACGTCAAA CATTCTGCTT CATCTGCGCC 
CAAATTTTAC GCGATAAGCA GTGCGACGGG ATTCAGTTCC GATATTTCTT TGCTACTCCT 
CTTCAGCAGT TGGCTCAAGA TAAAGTTCCA AATGTCCGAT TGGCATCTTG CGAGGCGTTA 
AGTGTGTGGA GAAAAACTTT GATCGCTGCT CGCGCTCAGG AAAGAAGAGG TCAATCTCCG 
ATCTCTTCGC CAGCATCAGA ACCATCAGCT ATCCGTCGTG ACATCAATCT TGTTTCCGAT 
ATGATGGTCA AACTCTCCAA CGATTCTGAT ATTGATGCGG CATTCATTGC AAAGAAGTGT 
CAAGGTTTAA CAGATGATCA TCAGCCAGTT GATGTTGCTT CACGAACTAC ACGGATGCGT 
GAACGAGAAG AACAGTTCTT TGGAGATATT ATTCTTAGCT ATTCTCCTGG TTGTAATGCA 
AGAGTTTCTG GTAAAGAAAT TAAACAGTTG ATCCGGCATG ATCAAGGAGC AGTTCAGAAA 
GCAGCTCCAC TTCAATCAAT GGAAGATTTT GGAGATGAGA TATTGGCTCA TGCGGCAGAT 
TCGGTGAATA TTCAAACACC TCCGGAAGTA GTTGCTACTG TTCAACGAGT CTCAATTACA 
AATGAACAAT TTGAGGATAA CACTGAAGCG GTTGATATGG AAATTGAAGA GGATGAAGAT 
GAAAACTCTA CAGAGAAGAA GGAAAACACT GAAAAAACCG CAGAGGAAGA ATCTGAGAAT 
GTTAAGAATA CTGAGGATGA ACTGGATATA CCTATGGATC CTGCACCTAC TCTTCCACCA 
CCAGTGACAT CAAAAAATCA ATCTTCATCA CCTATTACCG CCTCAAGTTC AGATAACAAC 
GAGACAAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021389.1
CDS	50..4144
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001026218.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026218.4

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTACATGGAA 
AACTCTGGTC CAAAGTTCAA CACTGATGAA GATTCAACGA ATGATTCAGA TAGTGACGAT 
GAGGATAATG ATTTTGAAAA GTCTTTTGTT GCTGACGAAG ATGACGGAGA ACAAGACGAT 
AGTCCATTAT CAATTGAAAT TAAATCATCT TCTTCGTCAG ACTTTGAATC AGTTTCGGCA 
GCCACTCCAA CATCTCAGGA GCCAGTATCT AATGAGTTTT CATTGACTTA TTGGTCGTCA 
AACTATGCTA TTTCGTCTAT CGAAGATGAG CTCAAACCTT TTGGATCCTC ATCCTCATTT 
ACCAATTCTC CAACTTCTAA CAGTTATTTG GAGAGCCGAT TCGATGTGAT GAAGGTTAAT 
CTTTCGCCAA GAGCTCGATC AGTGTCATTT GGAACTAATG GATCTCCGAT ATCCACTTCA 
CCACGAAGAA AAGCCAACAG TACATCTGAA CCATCTCCAC CAAACACCGG AAACTTGAAA 
AGCGATGACT CCATTGTAAT GCTCAATACG GAAGAAAAGG AGAAACTGGG AATTTTAGAT 
TTCGATGAAA TGAACATGTC AATATCATCG AATTCATCGA TTCATGGAGA AGCCGCAATG 
ACGGAAGATT CGGATATTAG TCTAACTTCG ATTGAACAGG CAGCACTCCA ACATGTTCCA 
GTAGATCTTG TTGAATCTTA TATGAGAGTC ATGGGGCCGA TTGGAGGTAG TGGAAGCGGA 
GGTGGTGGTA ATGGAAGTTC TACAACCATA GCTACTGGAG ATCCATCGCT TTGCGCGGAG 
ACCTATCGTC ATTGTGCTCA CAACTTTCCC GCAGTGTGCT ATACGCTTGG ACGTGCTGCA 
TGGCCTCGGC TTAAGCTTGT ATTCCGGAAG CTTGCGATGG ATGAGCAGGC TCGAGTTCGT 
CAGTCGATAT CTCATTCGAT TCATGAAATA GCAAATATGC TTGGACAGGA GATCACAGAT 
GAAGATTTGT TGCCAGTTTT CTATGATTTG AGAAACGATC AAAATCCAGA CGTTCGCAAT 
GGAATATTAA TTCATTTGTA TGATTTCGTG AAGTTCCTGT CGCCAGAAAA GCGTGACGAG 
ATGATTCTTT CCCTTCCACA ATTCTTCCCA ATCGGAGCTC AACCAGGAAA TCAGGCACAG 
AATGGTGATT GGAGATCTAG ATTTGAACTT ATATCTCAAC TTTCGAAGCT GTGCTCTCTG 
TACAGTATTC AAGATGTCAA TTTTCACATG TCTGGAATTG CTCTTACATT GGCTGATGAT 
CGTGTCGCAG AAGTTCGACG TGAAGCTGTG ATGCTTGTTT CAACGATCGT CGGAGCCCTT 
GTTACAGCTG AATGGGATAA CATCAAAGGA GTTCGGGATA TTGAAAATGG ACATGTTGAT 
GTGAAAAAGA AGAGTAACAA AACTGATTTT TTGTCCGAAC AATTTGTCGA TGATATTGTA 
TCCAGTTTTG CAAAAACTCA GAAATGGACA AGACGTCAAA CATTCTGCTT CATCTGCGCC 
CAAATTTTAC GCGATAAGCA GTGCGACGGG ATTCAGTTCC GATATTTCTT TGCTACTCCT 
CTTCAGCAGT TGGCTCAAGA TAAAGTTCCA AATGTCCGAT TGGCATCTTG CGAGGCGTTA 
AGTGTGTGGA GAAAAACTTT GATCGCTGCT CGCGCTCAGG AAAGAAGAGG TCAATCTCCG 
ATCTCTTCGC CAGCATCAGA ACCATCAGCT ATCCGTCGTG ACATCAATCT TGTTTCCGAT 
ATGATGGTCA AACTCTCCAA CGATTCTGAT ATTGATGCGG CATTCATTGC AAAGAAGTGT 
CAAGGTTTAA CAGATGATCA TCAGCCAGTT GATGTTGCTT CACGAACTAC ACGGATGCGT 
GAACGAGAAG AACAGTTCTT TGGAGATATT ATTCTTAGCT ATTCTCCTGG TTGTAATGCA 
AGAGTTTCTG GTAAAGAAAT TAAACAGTTG ATCCGGCATG ATCAAGGAGC AGTTCAGAAA 
GCAGCTCCAC TTCAATCAAT GGAAGATTTT GGAGATGAGA TATTGGCTCA TGCGGCAGAT 
TCGGTGAATA TTCAAACACC TCCGGAAGTA GTTGCTACTG TTCAACGAGT CTCAATTACA 
AATGAACAAT TTGAGGATAA CACTGAAGCG GTTGATATGG AAATTGAAGA GGATGAAGAT 
GAAAACTCTA CAGAGAAGAA GGAAAACACT GAAAAAACCG CAGAGGAAGA ATCTGAGAAT 
GTTAAGAATA CTGAGGATGA ACTGGATATA CCTATGGATC CTGCACCTAC TCTTCCACCA 
CCAGTGACAT CAAAAAATCA ATCTTCATCA CCTATTACCG CCTCAAGTTC AGATAACAAC 
GAGACAAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001026218.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021389.1 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

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CloneID	OCa335886
Clone ID Related Accession (Same CDS sequence)	NM_001026218.4 , NM_001026218.3
Accession Version	NM_001026218.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026218.2. COMPLETENESS: incomplete on both ends.

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTACATGGAA 
AACTCTGGTC CAAAGTTCAA CACTGATGAA GATTCAACGA ATGATTCAGA TAGTGACGAT 
GAGGATAATG ATTTTGAAAA GTCTTTTGTT GCTGACGAAG ATGACGGAGA ACAAGACGAT 
AGTCCATTAT CAATTGAAAT TAAATCATCT TCTTCGTCAG ACTTTGAATC AGTTTCGGCA 
GCCACTCCAA CATCTCAGGA GCCAGTATCT AATGAGTTTT CATTGACTTA TTGGTCGTCA 
AACTATGCTA TTTCGTCTAT CGAAGATGAG CTCAAACCTT TTGGATCCTC ATCCTCATTT 
ACCAATTCTC CAACTTCTAA CAGTTATTTG GAGAGCCGAT TCGATGTGAT GAAGGTTAAT 
CTTTCGCCAA GAGCTCGATC AGTGTCATTT GGAACTAATG GATCTCCGAT ATCCACTTCA 
CCACGAAGAA AAGCCAACAG TACATCTGAA CCATCTCCAC CAAACACCGG AAACTTGAAA 
AGCGATGACT CCATTGTAAT GCTCAATACG GAAGAAAAGG AGAAACTGGG AATTTTAGAT 
TTCGATGAAA TGAACATGTC AATATCATCG AATTCATCGA TTCATGGAGA AGCCGCAATG 
ACGGAAGATT CGGATATTAG TCTAACTTCG ATTGAACAGG CAGCACTCCA ACATGTTCCA 
GTAGATCTTG TTGAATCTTA TATGAGAGTC ATGGGGCCGA TTGGAGGTAG TGGAAGCGGA 
GGTGGTGGTA ATGGAAGTTC TACAACCATA GCTACTGGAG ATCCATCGCT TTGCGCGGAG 
ACCTATCGTC ATTGTGCTCA CAACTTTCCC GCAGTGTGCT ATACGCTTGG ACGTGCTGCA 
TGGCCTCGGC TTAAGCTTGT ATTCCGGAAG CTTGCGATGG ATGAGCAGGC TCGAGTTCGT 
CAGTCGATAT CTCATTCGAT TCATGAAATA GCAAATATGC TTGGACAGGA GATCACAGAT 
GAAGATTTGT TGCCAGTTTT CTATGATTTG AGAAACGATC AAAATCCAGA CGTTCGCAAT 
GGAATATTAA TTCATTTGTA TGATTTCGTG AAGTTCCTGT CGCCAGAAAA GCGTGACGAG 
ATGATTCTTT CCCTTCCACA ATTCTTCCCA ATCGGAGCTC AACCAGGAAA TCAGGCACAG 
AATGGTGATT GGAGATCTAG ATTTGAACTT ATATCTCAAC TTTCGAAGCT GTGCTCTCTG 
TACAGTATTC AAGATGTCAA TTTTCACATG TCTGGAATTG CTCTTACATT GGCTGATGAT 
CGTGTCGCAG AAGTTCGACG TGAAGCTGTG ATGCTTGTTT CAACGATCGT CGGAGCCCTT 
GTTACAGCTG AATGGGATAA CATCAAAGGA GTTCGGGATA TTGAAAATGG ACATGTTGAT 
GTGAAAAAGA AGAGTAACAA AACTGATTTT TTGTCCGAAC AATTTGTCGA TGATATTGTA 
TCCAGTTTTG CAAAAACTCA GAAATGGACA AGACGTCAAA CATTCTGCTT CATCTGCGCC 
CAAATTTTAC GCGATAAGCA GTGCGACGGG ATTCAGTTCC GATATTTCTT TGCTACTCCT 
CTTCAGCAGT TGGCTCAAGA TAAAGTTCCA AATGTCCGAT TGGCATCTTG CGAGGCGTTA 
AGTGTGTGGA GAAAAACTTT GATCGCTGCT CGCGCTCAGG AAAGAAGAGG TCAATCTCCG 
ATCTCTTCGC CAGCATCAGA ACCATCAGCT ATCCGTCGTG ACATCAATCT TGTTTCCGAT 
ATGATGGTCA AACTCTCCAA CGATTCTGAT ATTGATGCGG CATTCATTGC AAAGAAGTGT 
CAAGGTTTAA CAGATGATCA TCAGCCAGTT GATGTTGCTT CACGAACTAC ACGGATGCGT 
GAACGAGAAG AACAGTTCTT TGGAGATATT ATTCTTAGCT ATTCTCCTGG TTGTAATGCA 
AGAGTTTCTG GTAAAGAAAT TAAACAGTTG ATCCGGCATG ATCAAGGAGC AGTTCAGAAA 
GCAGCTCCAC TTCAATCAAT GGAAGATTTT GGAGATGAGA TATTGGCTCA TGCGGCAGAT 
TCGGTGAATA TTCAAACACC TCCGGAAGTA GTTGCTACTG TTCAACGAGT CTCAATTACA 
AATGAACAAT TTGAGGATAA CACTGAAGCG GTTGATATGG AAATTGAAGA GGATGAAGAT 
GAAAACTCTA CAGAGAAGAA GGAAAACACT GAAAAAACCG CAGAGGAAGA ATCTGAGAAT 
GTTAAGAATA CTGAGGATGA ACTGGATATA CCTATGGATC CTGCACCTAC TCTTCCACCA 
CCAGTGACAT CAAAAAATCA ATCTTCATCA CCTATTACCG CCTCAAGTTC AGATAACAAC 
GAGACAAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021389.1
CDS	1..4095
Translation	MDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001026218.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026218.3

ORF Insert Sequence:

ATGGATAAAA GAAATACAGG GTTCTCAAAT TTGCAACAAC TTGGCGGTGC TATCTTTATC 
GATGATGCTG AAGATGAGCC ACCAAGAGTC AAAAGTTCAG AAGATTCGAG ACCGGCTCCA 
CATTGGCTGG ACAGTCCAAG AGACAGCACG ACGCTTCCAG ATATCATTGC TTCAACATAT 
AGTGATTTCA TAGATGAAGC AGAATATGCG ACAAAACAGA TGCTGGCCAT CTTGGAAACA 
CTGAATCCTA TCGAAGAGTA CATTGAACTA TTTGAGAGCT CATTCAAAGC CATAGAGGTC 
CGATTGCCAT TCGATGTGAT TTTAAATGAT GAAAAAAAAC CAGATTGGGA TGCGATTCGG 
CGAAAACTGA TTATGGGCAG TCTGACAGTG AATACAACAT CTGAAAAGCC AGGAATAACC 
ACTAATCAGA TGACAGCGAT ATTTGACGCT ATTCCATTTC TTTTTGATGC CGTTGCTTGC 
AATCCGGAAC TTTATGAATA TCAGAATACT TTATCCATGA TTGTGATGAT TGCGATGACC 
GGAAACTCTA TGATCCGACG ACAGAGCATC AATGCAGTAC AGGCACTTAT GGAAAGGAAT 
ATGCTTGATC AGGATTTCAT GAGAGATGGC GTTGTTCCCG GACTCTTTAA CATTTATCAA 
TCAGGTGTCG GTGCGTTCCA GTCAAACGAT CTAAGGATGG AGTGTGTCCA GGCACTTTGC 
CAGGTTATCT CGTATGATGC GATCCACGAT CGACGATGGT TATTCGATAA TTTCCTGCCA 
CGTTTTTCAC AAATGCTCAA AGATCCGACG ATGCATGTCA GGAAGAGTGC CCTTCATATT 
TTTGGAAATC TTGGAAACAT GTTTGGACAG AAATTCACCG AAGTGTTCTT GGTTCCCCAC 
CTGATTACAC TCTCGTGCGA TATGACATGG GCGGTTCGGA AAGTTGCATG CGAGATATTT 
GTGAAAGTGG CTGAATGTGC TTCAAATCAA GTGAGAATTG ACATTTTGTC ACCTAATTTC 
GTGAGATTAT TGAATGATCC GAACAAATGG GTGTCGTTCA TTGCTTATCA ACAACTTGGA 
CCATTCATTT CACTATTTGC TAATCCGGAT ATTACGGGAC TTGAACTTCG AAATGGACAA 
GTCGTTGTCA GAGATCCAGT AGTATTAGAA CCATCAGTAA CTGAACAGGA AGATCCATCA 
AATTCTACAT TTTCGGAAGA CACATCGGAT GATTTTAGAA CACTTCCTAA AGATACGTGG 
CTGCCTGAAG TTGATTTTAA TGATGACTTT GAATATCAAT TGGGATCAAT CGATAATACA 
CAGAATCAAA AGGAATTGAC AACTGTGACG ACAGCATTGG TGAAAACTCC GAAACGAGGG 
GAACGAGTTA TGGACAGCGT TCTGTCTGGA ATCAACAAAA TGTTTGGATC GTTGGAACGA 
AGCTCGCCGA AATCAAAAGA AGTCGAAAAA ATTGCTGCCA CATTTTCAAG TCCATCGAAA 
CGATCACCAC TATCACTTCA CAGTGCAGTC AGTCTGATTG ATAAATTCAG CAAAAGAAGC 
AATCCGAACA ATCCACAGCG ATTCCCAGTA TTTGATTCTG CCAACTCGGA TTCCACATTA 
TCATCTAATT CCTCTAACAA TAACAGCTCA GAGCAGCAGA ATTCGACGGG AATCGCTGCA 
AAGTGCAGTT CAACTGATGA TTTGACTAAA TTAGGACTTG AAGACGATGA CTACATGGAA 
AACTCTGGTC CAAAGTTCAA CACTGATGAA GATTCAACGA ATGATTCAGA TAGTGACGAT 
GAGGATAATG ATTTTGAAAA GTCTTTTGTT GCTGACGAAG ATGACGGAGA ACAAGACGAT 
AGTCCATTAT CAATTGAAAT TAAATCATCT TCTTCGTCAG ACTTTGAATC AGTTTCGGCA 
GCCACTCCAA CATCTCAGGA GCCAGTATCT AATGAGTTTT CATTGACTTA TTGGTCGTCA 
AACTATGCTA TTTCGTCTAT CGAAGATGAG CTCAAACCTT TTGGATCCTC ATCCTCATTT 
ACCAATTCTC CAACTTCTAA CAGTTATTTG GAGAGCCGAT TCGATGTGAT GAAGGTTAAT 
CTTTCGCCAA GAGCTCGATC AGTGTCATTT GGAACTAATG GATCTCCGAT ATCCACTTCA 
CCACGAAGAA AAGCCAACAG TACATCTGAA CCATCTCCAC CAAACACCGG AAACTTGAAA 
AGCGATGACT CCATTGTAAT GCTCAATACG GAAGAAAAGG AGAAACTGGG AATTTTAGAT 
TTCGATGAAA TGAACATGTC AATATCATCG AATTCATCGA TTCATGGAGA AGCCGCAATG 
ACGGAAGATT CGGATATTAG TCTAACTTCG ATTGAACAGG CAGCACTCCA ACATGTTCCA 
GTAGATCTTG TTGAATCTTA TATGAGAGTC ATGGGGCCGA TTGGAGGTAG TGGAAGCGGA 
GGTGGTGGTA ATGGAAGTTC TACAACCATA GCTACTGGAG ATCCATCGCT TTGCGCGGAG 
ACCTATCGTC ATTGTGCTCA CAACTTTCCC GCAGTGTGCT ATACGCTTGG ACGTGCTGCA 
TGGCCTCGGC TTAAGCTTGT ATTCCGGAAG CTTGCGATGG ATGAGCAGGC TCGAGTTCGT 
CAGTCGATAT CTCATTCGAT TCATGAAATA GCAAATATGC TTGGACAGGA GATCACAGAT 
GAAGATTTGT TGCCAGTTTT CTATGATTTG AGAAACGATC AAAATCCAGA CGTTCGCAAT 
GGAATATTAA TTCATTTGTA TGATTTCGTG AAGTTCCTGT CGCCAGAAAA GCGTGACGAG 
ATGATTCTTT CCCTTCCACA ATTCTTCCCA ATCGGAGCTC AACCAGGAAA TCAGGCACAG 
AATGGTGATT GGAGATCTAG ATTTGAACTT ATATCTCAAC TTTCGAAGCT GTGCTCTCTG 
TACAGTATTC AAGATGTCAA TTTTCACATG TCTGGAATTG CTCTTACATT GGCTGATGAT 
CGTGTCGCAG AAGTTCGACG TGAAGCTGTG ATGCTTGTTT CAACGATCGT CGGAGCCCTT 
GTTACAGCTG AATGGGATAA CATCAAAGGA GTTCGGGATA TTGAAAATGG ACATGTTGAT 
GTGAAAAAGA AGAGTAACAA AACTGATTTT TTGTCCGAAC AATTTGTCGA TGATATTGTA 
TCCAGTTTTG CAAAAACTCA GAAATGGACA AGACGTCAAA CATTCTGCTT CATCTGCGCC 
CAAATTTTAC GCGATAAGCA GTGCGACGGG ATTCAGTTCC GATATTTCTT TGCTACTCCT 
CTTCAGCAGT TGGCTCAAGA TAAAGTTCCA AATGTCCGAT TGGCATCTTG CGAGGCGTTA 
AGTGTGTGGA GAAAAACTTT GATCGCTGCT CGCGCTCAGG AAAGAAGAGG TCAATCTCCG 
ATCTCTTCGC CAGCATCAGA ACCATCAGCT ATCCGTCGTG ACATCAATCT TGTTTCCGAT 
ATGATGGTCA AACTCTCCAA CGATTCTGAT ATTGATGCGG CATTCATTGC AAAGAAGTGT 
CAAGGTTTAA CAGATGATCA TCAGCCAGTT GATGTTGCTT CACGAACTAC ACGGATGCGT 
GAACGAGAAG AACAGTTCTT TGGAGATATT ATTCTTAGCT ATTCTCCTGG TTGTAATGCA 
AGAGTTTCTG GTAAAGAAAT TAAACAGTTG ATCCGGCATG ATCAAGGAGC AGTTCAGAAA 
GCAGCTCCAC TTCAATCAAT GGAAGATTTT GGAGATGAGA TATTGGCTCA TGCGGCAGAT 
TCGGTGAATA TTCAAACACC TCCGGAAGTA GTTGCTACTG TTCAACGAGT CTCAATTACA 
AATGAACAAT TTGAGGATAA CACTGAAGCG GTTGATATGG AAATTGAAGA GGATGAAGAT 
GAAAACTCTA CAGAGAAGAA GGAAAACACT GAAAAAACCG CAGAGGAAGA ATCTGAGAAT 
GTTAAGAATA CTGAGGATGA ACTGGATATA CCTATGGATC CTGCACCTAC TCTTCCACCA 
CCAGTGACAT CAAAAAATCA ATCTTCATCA CCTATTACCG CCTCAAGTTC AGATAACAAC 
GAGACAAGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001128984.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122456.2 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Price & Availability↑

CloneID	OCa335394
Clone ID Related Accession (Same CDS sequence)	NM_001128984.2 , NM_001128984.3
Accession Version	NM_001128984.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001128984.1. COMPLETENESS: incomplete on both ends.

ATGATAAAGA GCCAATTTGC TACCTTGATC CGTGTGCTGA TCGATTTTCA GAAATTTTGG 
GTTTTTCTCT CAATTTTTTG CACATTTTTA GTGGTTTTCA AGTTCGACAA TCTTATGTTT 
CAAAAAGTTC GGCGAGTTTT CCAACAAAAT CGGAAAAACT CAGAAAGTAT TCAACCCAAG 
TTATCAACAG TTAGCGGATC AAATAGTGGC GAAGGAATGG ATAAAAGAAA TACAGGGTTC 
TCAAATTTGC AACAACTTGG CGGTGCTATC TTTATCGATG ATGCTGAAGA TGAGCCACCA 
AGAGTCAAAA GTTCAGAAGA TTCGAGACCG GCTCCACATT GGCTGGACAG TCCAAGAGAC 
AGCACGACGC TTCCAGATAT CATTGCTTCA ACATATAGTG ATTTCATAGA TGAAGCAGAA 
TATGCGACAA AACAGATGCT GGCCATCTTG GAAACACTGA ATCCTATCGA AGAGTACATT 
GAACTATTTG AGAGCTCATT CAAAGCCATA GAGGTCCGAT TGCCATTCGA TGTGATTTTA 
AATGATGAAA AAAAACCAGA TTGGGATGCG ATTCGGCGAA AACTGATTAT GGGCAGTCTG 
ACAGTGAATA CAACATCTGA AAAGCCAGGA ATAACCACTA ATCAGATGAC AGCGATATTT 
GACGCTATTC CATTTCTTTT TGATGCCGTT GCTTGCAATC CGGAACTTTA TGAATATCAG 
AATACTTTAT CCATGATTGT GATGATTGCG ATGACCGGAA ACTCTATGAT CCGACGACAG 
AGCATCAATG CAGTACAGGC ACTTATGGAA AGGAATATGC TTGATCAGGA TTTCATGAGA 
GATGGCGTTG TTCCCGGACT CTTTAACATT TATCAATCAG GTGTCGGTGC GTTCCAGTCA 
AACGATCTAA GGATGGAGTG TGTCCAGGCA CTTTGCCAGG TTATCTCGTA TGATGCGATC 
CACGATCGAC GATGGTTATT CGATAATTTC CTGCCACGTT TTTCACAAAT GCTCAAAGAT 
CCGACGATGC ATGTCAGGAA GAGTGCCCTT CATATTTTTG GAAATCTTGG AAACATGTTT 
GGACAGAAAT TCACCGAAGT GTTCTTGGTT CCCCACCTGA TTACACTCTC GTGCGATATG 
ACATGGGCGG TTCGGAAAGT TGCATGCGAG ATATTTGTGA AAGTGGCTGA ATGTGCTTCA 
AATCAAGTGA GAATTGACAT TTTGTCACCT AATTTCGTGA GATTATTGAA TGATCCGAAC 
AAATGGGTGT CGTTCATTGC TTATCAACAA CTTGGACCAT TCATTTCACT ATTTGCTAAT 
CCGGATATTA CGGGACTTGA ACTTCGAAAT GGACAAGTCG TTGTCAGAGA TCCAGTAGTA 
TTAGAACCAT CAGTAACTGA ACAGGAAGAT CCATCAAATT CTACATTTTC GGAAGACACA 
TCGGATGATT TTAGAACACT TCCTAAAGAT ACGTGGCTGC CTGAAGTTGA TTTTAATGAT 
GACTTTGAAT ATCAATTGGG ATCAATCGAT AATACACAGA ATCAAAAGGA ATTGACAACT 
GTGACGACAG CATTGGTGAA AACTCCGAAA CGAGGGGAAC GAGTTATGGA CAGCGTTCTG 
TCTGGAATCA ACAAAATGTT TGGATCGTTG GAACGAAGCT CGCCGAAATC AAAAGAAGTC 
GAAAAAATTG CTGCCACATT TTCAAGTCCA TCGAAACGAT CACCACTATC ACTTCACAGT 
GCAGTCAGTC TGATTGATAA ATTCAGCAAA AGAAGCAATC CGAACAATCC ACAGCGATTC 
CCAGTATTTG ATTCTGCCAA CTCGGATTCC ACATTATCAT CTAATTCCTC TAACAATAAC 
AGCTCAGAGC AGCAGAATTC GACGGGAATC GCTGCAAAGT GCAGTTCAAC TGATGATTTG 
ACTAAATTAG GACTTGAAGA CGATGACTCA CCAGATGCAG CTGAAGATTC CGATGATTTC 
ACATCGGAAA CACGGACGAC AAGTCCACGT GGTCGGCGTA TTGCCACCAA CGCGTGGTCG 
AAGCTTGATA TTCGGCAGAA GATATTTCTC CGGCGACTTG GAGCTCTACC ACCGAGCTTT 
CCGAAGAAAA AGGCGACACA CAGCAGCAGC AGCAGTCCGA GAAAATCACC AACAAGATCG 
CCACTGCTGT CGTCGCGAAA AAAGGGACTT TTGTTTGATT TTGCGGCGCC TATTGAAATC 
AAATCATCGT CTTCTGATCA TTCGGAGACA ATTCCAGTCC GGACAGCCAA TCAACAGCAA 
GAAAAAGCAG ATGATGATAA GCTGAATACC GATGAACGGC AGCAATACAT GGAAAACTCT 
GGTCCAAAGT TCAACACTGA TGAAGATTCA ACGAATGATT CAGATAGTGA CGATGAGGAT 
AATGATTTTG AAAAGTCTTT TGTTGCTGAC GAAGATGACG GAGAACAAGA CGATAGTCCA 
TTATCAATTG AAATTAAATC ATCTTCTTCG TCAGACTTTG AATCAGTTTC GGCAGCCACT 
CCAACATCTC AGGAGCCAGT ATCTAATGAG TTTTCATTGA CTTATTGGTC GTCAAACTAT 
GCTATTTCGT CTATCGAAGA TGAGCTCAAA CCTTTTGGAT CCTCATCCTC ATTTACCAAT 
TCTCCAACTT CTAACAGTTA TTTGGAGAGC CGATTCGATG TGATGAAGGT TAATCTTTCG 
CCAAGAGCTC GATCAGTGTC ATTTGGAACT AATGGATCTC CGATATCCAC TTCACCACGA 
AGAAAAGCCA ACAGTACATC TGAACCATCT CCACCAAACA CCGGAAACTT GAAAAGCGAT 
GACTCCATTG TAATGCTCAA TACGGAAGAA AAGGAGAAAC TGGGAATTTT AGATTTCGAT 
GAAATGAACA TGTCAATATC ATCGAATTCA TCGATTCATG GAGAAGCCGC AATGACGGAA 
GATTCGGATA TTAGTCTAAC TTCGATTGAA CAGGCAGCAC TCCAACATGT TCCAGTAGAT 
CTTGTTGAAT CTTATATGAG AGTCATGGGG CCGATTGGAG GTAGTGGAAG CGGAGGTGGT 
GGTAATGGAA GTTCTACAAC CATAGCTACT GGAGATCCAT CGCTTTGCGC GGAGACCTAT 
CGTCATTGTG CTCACAACTT TCCCGCAGTG TGCTATACGC TTGGACGTGC TGCATGGCCT 
CGGCTTAAGC TTGTATTCCG GAAGCTTGCG ATGGATGAGC AGGCTCGAGT TCGTCAGTCG 
ATATCTCATT CGATTCATGA AATAGCAAAT ATGCTTGGAC AGGAGATCAC AGATGAAGAT 
TTGTTGCCAG TTTTCTATGA TTTGAGAAAC GATCAAAATC CAGACGTTCG CAATGGAATA 
TTAATTCATT TGTATGATTT CGTGAAGTTC CTGTCGCCAG AAAAGCGTGA CGAGATGATT 
CTTTCCCTTC CACAATTCTT CCCAATCGGA GCTCAACCAG GAAATCAGGC ACAGAATGGT 
GATTGGAGAT CTAGATTTGA ACTTATATCT CAACTTTCGA AGCTGTGCTC TCTGTACAGT 
ATTCAAGATG TCAATTTTCA CATGTCTGGA ATTGCTCTTA CATTGGCTGA TGATCGTGTC 
GCAGAAGTTC GACGTGAAGC TGTGATGCTT GTTTCAACGA TCGTCGGAGC CCTTGTTACA 
GCTGAATGGG ATAACATCAA AGGAGTTCGG GATATTGAAA ATGGACATGT TGATGTGAAA 
AAGAAGAGTA ACAAAACTGA TTTTTTGTCC GAACAATTTG TCGATGATAT TGTATCCAGT 
TTTGCAAAAA CTCAGAAATG GACAAGACGT CAAACATTCT GCTTCATCTG CGCCCAAATT 
TTACGCGATA AGCAGTGCGA CGGGATTCAG TTCCGATATT TCTTTGCTAC TCCTCTTCAG 
CAGTTGGCTC AAGATAAAGT TCCAAATGTC CGATTGGCAT CTTGCGAGGC GTTAAGTGTG 
TGGAGAAAAA CTTTGATCGC TGCTCGCGCT CAGGAAAGAA GAGGTCAATC TCCGATCTCT 
TCGCCAGCAT CAGAACCATC AGCTATCCGT CGTGACATCA ATCTTGTTTC CGATATGATG 
GTCAAACTCT CCAACGATTC TGATATTGAT GCGGCATTCA TTGCAAAGAA GTGTCAAGGT 
TTAACAGATG ATCATCAGCC AGTTGATGTT GCTTCACGAA CTACACGGAT GCGTGAACGA 
GAAGAACAGT TCTTTGGAGA TATTATTCTT AGCTATTCTC CTGGTTGTAA TGCAAGAGTT 
TCTGGTAAAG AAATTAAACA GTTGATCCGG CATGATCAAG GAGCAGTTCA GAAAGCAGCT 
CCACTTCAAT CAATGGAAGA TTTTGGAGAT GAGATATTGG CTCATGCGGC AGATTCGGTG 
AATATTCAAA CACCTCCGGA AGTAGTTGCT ACTGTTCAAC GAGTCTCAAT TACAAATGAA 
CAATTTGAGG ATAACACTGA AGCGGTTGAT ATGGAAATTG AAGAGGATGA AGATGAAAAC 
TCTACAGAGA AGAAGGAAAA CACTGAAAAA ACCGCAGAGG AAGAATCTGA GAATGTTAAG 
AATACTGAGG ATGAACTGGA TATACCTATG GATCCTGCAC CTACTCTTCC ACCACCAGTG 
ACATCAAAAA ATCAATCTTC ATCACCTATT ACCGCCTCAA GTTCAGATAA CAACGAGACA 
AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122456.2
CDS	1..4689
Translation	MIKSQFATLIRVLIDFQKFWVFLSIFCTFLVVFKFDNLMFQKVRRVFQQNRKNSESIQPKLSTVSGSNSGEGMDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001128984.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128984.2

ORF Insert Sequence:

ATGATAAAGA GCCAATTTGC TACCTTGATC CGTGTGCTGA TCGATTTTCA GAAATTTTGG 
GTTTTTCTCT CAATTTTTTG CACATTTTTA GTGGTTTTCA AGTTCGACAA TCTTATGTTT 
CAAAAAGTTC GGCGAGTTTT CCAACAAAAT CGGAAAAACT CAGAAAGTAT TCAACCCAAG 
TTATCAACAG TTAGCGGATC AAATAGTGGC GAAGGAATGG ATAAAAGAAA TACAGGGTTC 
TCAAATTTGC AACAACTTGG CGGTGCTATC TTTATCGATG ATGCTGAAGA TGAGCCACCA 
AGAGTCAAAA GTTCAGAAGA TTCGAGACCG GCTCCACATT GGCTGGACAG TCCAAGAGAC 
AGCACGACGC TTCCAGATAT CATTGCTTCA ACATATAGTG ATTTCATAGA TGAAGCAGAA 
TATGCGACAA AACAGATGCT GGCCATCTTG GAAACACTGA ATCCTATCGA AGAGTACATT 
GAACTATTTG AGAGCTCATT CAAAGCCATA GAGGTCCGAT TGCCATTCGA TGTGATTTTA 
AATGATGAAA AAAAACCAGA TTGGGATGCG ATTCGGCGAA AACTGATTAT GGGCAGTCTG 
ACAGTGAATA CAACATCTGA AAAGCCAGGA ATAACCACTA ATCAGATGAC AGCGATATTT 
GACGCTATTC CATTTCTTTT TGATGCCGTT GCTTGCAATC CGGAACTTTA TGAATATCAG 
AATACTTTAT CCATGATTGT GATGATTGCG ATGACCGGAA ACTCTATGAT CCGACGACAG 
AGCATCAATG CAGTACAGGC ACTTATGGAA AGGAATATGC TTGATCAGGA TTTCATGAGA 
GATGGCGTTG TTCCCGGACT CTTTAACATT TATCAATCAG GTGTCGGTGC GTTCCAGTCA 
AACGATCTAA GGATGGAGTG TGTCCAGGCA CTTTGCCAGG TTATCTCGTA TGATGCGATC 
CACGATCGAC GATGGTTATT CGATAATTTC CTGCCACGTT TTTCACAAAT GCTCAAAGAT 
CCGACGATGC ATGTCAGGAA GAGTGCCCTT CATATTTTTG GAAATCTTGG AAACATGTTT 
GGACAGAAAT TCACCGAAGT GTTCTTGGTT CCCCACCTGA TTACACTCTC GTGCGATATG 
ACATGGGCGG TTCGGAAAGT TGCATGCGAG ATATTTGTGA AAGTGGCTGA ATGTGCTTCA 
AATCAAGTGA GAATTGACAT TTTGTCACCT AATTTCGTGA GATTATTGAA TGATCCGAAC 
AAATGGGTGT CGTTCATTGC TTATCAACAA CTTGGACCAT TCATTTCACT ATTTGCTAAT 
CCGGATATTA CGGGACTTGA ACTTCGAAAT GGACAAGTCG TTGTCAGAGA TCCAGTAGTA 
TTAGAACCAT CAGTAACTGA ACAGGAAGAT CCATCAAATT CTACATTTTC GGAAGACACA 
TCGGATGATT TTAGAACACT TCCTAAAGAT ACGTGGCTGC CTGAAGTTGA TTTTAATGAT 
GACTTTGAAT ATCAATTGGG ATCAATCGAT AATACACAGA ATCAAAAGGA ATTGACAACT 
GTGACGACAG CATTGGTGAA AACTCCGAAA CGAGGGGAAC GAGTTATGGA CAGCGTTCTG 
TCTGGAATCA ACAAAATGTT TGGATCGTTG GAACGAAGCT CGCCGAAATC AAAAGAAGTC 
GAAAAAATTG CTGCCACATT TTCAAGTCCA TCGAAACGAT CACCACTATC ACTTCACAGT 
GCAGTCAGTC TGATTGATAA ATTCAGCAAA AGAAGCAATC CGAACAATCC ACAGCGATTC 
CCAGTATTTG ATTCTGCCAA CTCGGATTCC ACATTATCAT CTAATTCCTC TAACAATAAC 
AGCTCAGAGC AGCAGAATTC GACGGGAATC GCTGCAAAGT GCAGTTCAAC TGATGATTTG 
ACTAAATTAG GACTTGAAGA CGATGACTCA CCAGATGCAG CTGAAGATTC CGATGATTTC 
ACATCGGAAA CACGGACGAC AAGTCCACGT GGTCGGCGTA TTGCCACCAA CGCGTGGTCG 
AAGCTTGATA TTCGGCAGAA GATATTTCTC CGGCGACTTG GAGCTCTACC ACCGAGCTTT 
CCGAAGAAAA AGGCGACACA CAGCAGCAGC AGCAGTCCGA GAAAATCACC AACAAGATCG 
CCACTGCTGT CGTCGCGAAA AAAGGGACTT TTGTTTGATT TTGCGGCGCC TATTGAAATC 
AAATCATCGT CTTCTGATCA TTCGGAGACA ATTCCAGTCC GGACAGCCAA TCAACAGCAA 
GAAAAAGCAG ATGATGATAA GCTGAATACC GATGAACGGC AGCAATACAT GGAAAACTCT 
GGTCCAAAGT TCAACACTGA TGAAGATTCA ACGAATGATT CAGATAGTGA CGATGAGGAT 
AATGATTTTG AAAAGTCTTT TGTTGCTGAC GAAGATGACG GAGAACAAGA CGATAGTCCA 
TTATCAATTG AAATTAAATC ATCTTCTTCG TCAGACTTTG AATCAGTTTC GGCAGCCACT 
CCAACATCTC AGGAGCCAGT ATCTAATGAG TTTTCATTGA CTTATTGGTC GTCAAACTAT 
GCTATTTCGT CTATCGAAGA TGAGCTCAAA CCTTTTGGAT CCTCATCCTC ATTTACCAAT 
TCTCCAACTT CTAACAGTTA TTTGGAGAGC CGATTCGATG TGATGAAGGT TAATCTTTCG 
CCAAGAGCTC GATCAGTGTC ATTTGGAACT AATGGATCTC CGATATCCAC TTCACCACGA 
AGAAAAGCCA ACAGTACATC TGAACCATCT CCACCAAACA CCGGAAACTT GAAAAGCGAT 
GACTCCATTG TAATGCTCAA TACGGAAGAA AAGGAGAAAC TGGGAATTTT AGATTTCGAT 
GAAATGAACA TGTCAATATC ATCGAATTCA TCGATTCATG GAGAAGCCGC AATGACGGAA 
GATTCGGATA TTAGTCTAAC TTCGATTGAA CAGGCAGCAC TCCAACATGT TCCAGTAGAT 
CTTGTTGAAT CTTATATGAG AGTCATGGGG CCGATTGGAG GTAGTGGAAG CGGAGGTGGT 
GGTAATGGAA GTTCTACAAC CATAGCTACT GGAGATCCAT CGCTTTGCGC GGAGACCTAT 
CGTCATTGTG CTCACAACTT TCCCGCAGTG TGCTATACGC TTGGACGTGC TGCATGGCCT 
CGGCTTAAGC TTGTATTCCG GAAGCTTGCG ATGGATGAGC AGGCTCGAGT TCGTCAGTCG 
ATATCTCATT CGATTCATGA AATAGCAAAT ATGCTTGGAC AGGAGATCAC AGATGAAGAT 
TTGTTGCCAG TTTTCTATGA TTTGAGAAAC GATCAAAATC CAGACGTTCG CAATGGAATA 
TTAATTCATT TGTATGATTT CGTGAAGTTC CTGTCGCCAG AAAAGCGTGA CGAGATGATT 
CTTTCCCTTC CACAATTCTT CCCAATCGGA GCTCAACCAG GAAATCAGGC ACAGAATGGT 
GATTGGAGAT CTAGATTTGA ACTTATATCT CAACTTTCGA AGCTGTGCTC TCTGTACAGT 
ATTCAAGATG TCAATTTTCA CATGTCTGGA ATTGCTCTTA CATTGGCTGA TGATCGTGTC 
GCAGAAGTTC GACGTGAAGC TGTGATGCTT GTTTCAACGA TCGTCGGAGC CCTTGTTACA 
GCTGAATGGG ATAACATCAA AGGAGTTCGG GATATTGAAA ATGGACATGT TGATGTGAAA 
AAGAAGAGTA ACAAAACTGA TTTTTTGTCC GAACAATTTG TCGATGATAT TGTATCCAGT 
TTTGCAAAAA CTCAGAAATG GACAAGACGT CAAACATTCT GCTTCATCTG CGCCCAAATT 
TTACGCGATA AGCAGTGCGA CGGGATTCAG TTCCGATATT TCTTTGCTAC TCCTCTTCAG 
CAGTTGGCTC AAGATAAAGT TCCAAATGTC CGATTGGCAT CTTGCGAGGC GTTAAGTGTG 
TGGAGAAAAA CTTTGATCGC TGCTCGCGCT CAGGAAAGAA GAGGTCAATC TCCGATCTCT 
TCGCCAGCAT CAGAACCATC AGCTATCCGT CGTGACATCA ATCTTGTTTC CGATATGATG 
GTCAAACTCT CCAACGATTC TGATATTGAT GCGGCATTCA TTGCAAAGAA GTGTCAAGGT 
TTAACAGATG ATCATCAGCC AGTTGATGTT GCTTCACGAA CTACACGGAT GCGTGAACGA 
GAAGAACAGT TCTTTGGAGA TATTATTCTT AGCTATTCTC CTGGTTGTAA TGCAAGAGTT 
TCTGGTAAAG AAATTAAACA GTTGATCCGG CATGATCAAG GAGCAGTTCA GAAAGCAGCT 
CCACTTCAAT CAATGGAAGA TTTTGGAGAT GAGATATTGG CTCATGCGGC AGATTCGGTG 
AATATTCAAA CACCTCCGGA AGTAGTTGCT ACTGTTCAAC GAGTCTCAAT TACAAATGAA 
CAATTTGAGG ATAACACTGA AGCGGTTGAT ATGGAAATTG AAGAGGATGA AGATGAAAAC 
TCTACAGAGA AGAAGGAAAA CACTGAAAAA ACCGCAGAGG AAGAATCTGA GAATGTTAAG 
AATACTGAGG ATGAACTGGA TATACCTATG GATCCTGCAC CTACTCTTCC ACCACCAGTG 
ACATCAAAAA ATCAATCTTC ATCACCTATT ACCGCCTCAA GTTCAGATAA CAACGAGACA 
AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfr-1 ( NM_001128984.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001122456.2 Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Price & Availability↑

CloneID	OCa335394
Clone ID Related Accession (Same CDS sequence)	NM_001128984.2 , NM_001128984.3
Accession Version	NM_001128984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Serine/threonine-protein phosphatase 4 regulatory subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001128984.2. COMPLETENESS: incomplete on the 5' end.

ATGATAAAGA GCCAATTTGC TACCTTGATC CGTGTGCTGA TCGATTTTCA GAAATTTTGG 
GTTTTTCTCT CAATTTTTTG CACATTTTTA GTGGTTTTCA AGTTCGACAA TCTTATGTTT 
CAAAAAGTTC GGCGAGTTTT CCAACAAAAT CGGAAAAACT CAGAAAGTAT TCAACCCAAG 
TTATCAACAG TTAGCGGATC AAATAGTGGC GAAGGAATGG ATAAAAGAAA TACAGGGTTC 
TCAAATTTGC AACAACTTGG CGGTGCTATC TTTATCGATG ATGCTGAAGA TGAGCCACCA 
AGAGTCAAAA GTTCAGAAGA TTCGAGACCG GCTCCACATT GGCTGGACAG TCCAAGAGAC 
AGCACGACGC TTCCAGATAT CATTGCTTCA ACATATAGTG ATTTCATAGA TGAAGCAGAA 
TATGCGACAA AACAGATGCT GGCCATCTTG GAAACACTGA ATCCTATCGA AGAGTACATT 
GAACTATTTG AGAGCTCATT CAAAGCCATA GAGGTCCGAT TGCCATTCGA TGTGATTTTA 
AATGATGAAA AAAAACCAGA TTGGGATGCG ATTCGGCGAA AACTGATTAT GGGCAGTCTG 
ACAGTGAATA CAACATCTGA AAAGCCAGGA ATAACCACTA ATCAGATGAC AGCGATATTT 
GACGCTATTC CATTTCTTTT TGATGCCGTT GCTTGCAATC CGGAACTTTA TGAATATCAG 
AATACTTTAT CCATGATTGT GATGATTGCG ATGACCGGAA ACTCTATGAT CCGACGACAG 
AGCATCAATG CAGTACAGGC ACTTATGGAA AGGAATATGC TTGATCAGGA TTTCATGAGA 
GATGGCGTTG TTCCCGGACT CTTTAACATT TATCAATCAG GTGTCGGTGC GTTCCAGTCA 
AACGATCTAA GGATGGAGTG TGTCCAGGCA CTTTGCCAGG TTATCTCGTA TGATGCGATC 
CACGATCGAC GATGGTTATT CGATAATTTC CTGCCACGTT TTTCACAAAT GCTCAAAGAT 
CCGACGATGC ATGTCAGGAA GAGTGCCCTT CATATTTTTG GAAATCTTGG AAACATGTTT 
GGACAGAAAT TCACCGAAGT GTTCTTGGTT CCCCACCTGA TTACACTCTC GTGCGATATG 
ACATGGGCGG TTCGGAAAGT TGCATGCGAG ATATTTGTGA AAGTGGCTGA ATGTGCTTCA 
AATCAAGTGA GAATTGACAT TTTGTCACCT AATTTCGTGA GATTATTGAA TGATCCGAAC 
AAATGGGTGT CGTTCATTGC TTATCAACAA CTTGGACCAT TCATTTCACT ATTTGCTAAT 
CCGGATATTA CGGGACTTGA ACTTCGAAAT GGACAAGTCG TTGTCAGAGA TCCAGTAGTA 
TTAGAACCAT CAGTAACTGA ACAGGAAGAT CCATCAAATT CTACATTTTC GGAAGACACA 
TCGGATGATT TTAGAACACT TCCTAAAGAT ACGTGGCTGC CTGAAGTTGA TTTTAATGAT 
GACTTTGAAT ATCAATTGGG ATCAATCGAT AATACACAGA ATCAAAAGGA ATTGACAACT 
GTGACGACAG CATTGGTGAA AACTCCGAAA CGAGGGGAAC GAGTTATGGA CAGCGTTCTG 
TCTGGAATCA ACAAAATGTT TGGATCGTTG GAACGAAGCT CGCCGAAATC AAAAGAAGTC 
GAAAAAATTG CTGCCACATT TTCAAGTCCA TCGAAACGAT CACCACTATC ACTTCACAGT 
GCAGTCAGTC TGATTGATAA ATTCAGCAAA AGAAGCAATC CGAACAATCC ACAGCGATTC 
CCAGTATTTG ATTCTGCCAA CTCGGATTCC ACATTATCAT CTAATTCCTC TAACAATAAC 
AGCTCAGAGC AGCAGAATTC GACGGGAATC GCTGCAAAGT GCAGTTCAAC TGATGATTTG 
ACTAAATTAG GACTTGAAGA CGATGACTCA CCAGATGCAG CTGAAGATTC CGATGATTTC 
ACATCGGAAA CACGGACGAC AAGTCCACGT GGTCGGCGTA TTGCCACCAA CGCGTGGTCG 
AAGCTTGATA TTCGGCAGAA GATATTTCTC CGGCGACTTG GAGCTCTACC ACCGAGCTTT 
CCGAAGAAAA AGGCGACACA CAGCAGCAGC AGCAGTCCGA GAAAATCACC AACAAGATCG 
CCACTGCTGT CGTCGCGAAA AAAGGGACTT TTGTTTGATT TTGCGGCGCC TATTGAAATC 
AAATCATCGT CTTCTGATCA TTCGGAGACA ATTCCAGTCC GGACAGCCAA TCAACAGCAA 
GAAAAAGCAG ATGATGATAA GCTGAATACC GATGAACGGC AGCAATACAT GGAAAACTCT 
GGTCCAAAGT TCAACACTGA TGAAGATTCA ACGAATGATT CAGATAGTGA CGATGAGGAT 
AATGATTTTG AAAAGTCTTT TGTTGCTGAC GAAGATGACG GAGAACAAGA CGATAGTCCA 
TTATCAATTG AAATTAAATC ATCTTCTTCG TCAGACTTTG AATCAGTTTC GGCAGCCACT 
CCAACATCTC AGGAGCCAGT ATCTAATGAG TTTTCATTGA CTTATTGGTC GTCAAACTAT 
GCTATTTCGT CTATCGAAGA TGAGCTCAAA CCTTTTGGAT CCTCATCCTC ATTTACCAAT 
TCTCCAACTT CTAACAGTTA TTTGGAGAGC CGATTCGATG TGATGAAGGT TAATCTTTCG 
CCAAGAGCTC GATCAGTGTC ATTTGGAACT AATGGATCTC CGATATCCAC TTCACCACGA 
AGAAAAGCCA ACAGTACATC TGAACCATCT CCACCAAACA CCGGAAACTT GAAAAGCGAT 
GACTCCATTG TAATGCTCAA TACGGAAGAA AAGGAGAAAC TGGGAATTTT AGATTTCGAT 
GAAATGAACA TGTCAATATC ATCGAATTCA TCGATTCATG GAGAAGCCGC AATGACGGAA 
GATTCGGATA TTAGTCTAAC TTCGATTGAA CAGGCAGCAC TCCAACATGT TCCAGTAGAT 
CTTGTTGAAT CTTATATGAG AGTCATGGGG CCGATTGGAG GTAGTGGAAG CGGAGGTGGT 
GGTAATGGAA GTTCTACAAC CATAGCTACT GGAGATCCAT CGCTTTGCGC GGAGACCTAT 
CGTCATTGTG CTCACAACTT TCCCGCAGTG TGCTATACGC TTGGACGTGC TGCATGGCCT 
CGGCTTAAGC TTGTATTCCG GAAGCTTGCG ATGGATGAGC AGGCTCGAGT TCGTCAGTCG 
ATATCTCATT CGATTCATGA AATAGCAAAT ATGCTTGGAC AGGAGATCAC AGATGAAGAT 
TTGTTGCCAG TTTTCTATGA TTTGAGAAAC GATCAAAATC CAGACGTTCG CAATGGAATA 
TTAATTCATT TGTATGATTT CGTGAAGTTC CTGTCGCCAG AAAAGCGTGA CGAGATGATT 
CTTTCCCTTC CACAATTCTT CCCAATCGGA GCTCAACCAG GAAATCAGGC ACAGAATGGT 
GATTGGAGAT CTAGATTTGA ACTTATATCT CAACTTTCGA AGCTGTGCTC TCTGTACAGT 
ATTCAAGATG TCAATTTTCA CATGTCTGGA ATTGCTCTTA CATTGGCTGA TGATCGTGTC 
GCAGAAGTTC GACGTGAAGC TGTGATGCTT GTTTCAACGA TCGTCGGAGC CCTTGTTACA 
GCTGAATGGG ATAACATCAA AGGAGTTCGG GATATTGAAA ATGGACATGT TGATGTGAAA 
AAGAAGAGTA ACAAAACTGA TTTTTTGTCC GAACAATTTG TCGATGATAT TGTATCCAGT 
TTTGCAAAAA CTCAGAAATG GACAAGACGT CAAACATTCT GCTTCATCTG CGCCCAAATT 
TTACGCGATA AGCAGTGCGA CGGGATTCAG TTCCGATATT TCTTTGCTAC TCCTCTTCAG 
CAGTTGGCTC AAGATAAAGT TCCAAATGTC CGATTGGCAT CTTGCGAGGC GTTAAGTGTG 
TGGAGAAAAA CTTTGATCGC TGCTCGCGCT CAGGAAAGAA GAGGTCAATC TCCGATCTCT 
TCGCCAGCAT CAGAACCATC AGCTATCCGT CGTGACATCA ATCTTGTTTC CGATATGATG 
GTCAAACTCT CCAACGATTC TGATATTGAT GCGGCATTCA TTGCAAAGAA GTGTCAAGGT 
TTAACAGATG ATCATCAGCC AGTTGATGTT GCTTCACGAA CTACACGGAT GCGTGAACGA 
GAAGAACAGT TCTTTGGAGA TATTATTCTT AGCTATTCTC CTGGTTGTAA TGCAAGAGTT 
TCTGGTAAAG AAATTAAACA GTTGATCCGG CATGATCAAG GAGCAGTTCA GAAAGCAGCT 
CCACTTCAAT CAATGGAAGA TTTTGGAGAT GAGATATTGG CTCATGCGGC AGATTCGGTG 
AATATTCAAA CACCTCCGGA AGTAGTTGCT ACTGTTCAAC GAGTCTCAAT TACAAATGAA 
CAATTTGAGG ATAACACTGA AGCGGTTGAT ATGGAAATTG AAGAGGATGA AGATGAAAAC 
TCTACAGAGA AGAAGGAAAA CACTGAAAAA ACCGCAGAGG AAGAATCTGA GAATGTTAAG 
AATACTGAGG ATGAACTGGA TATACCTATG GATCCTGCAC CTACTCTTCC ACCACCAGTG 
ACATCAAAAA ATCAATCTTC ATCACCTATT ACCGCCTCAA GTTCAGATAA CAACGAGACA 
AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122456.2
CDS	1..4689
Translation	MIKSQFATLIRVLIDFQKFWVFLSIFCTFLVVFKFDNLMFQKVRRVFQQNRKNSESIQPKLSTVSGSNSGEGMDKRNTGFSNLQQLGGAIFIDDAEDEPPRVKSSEDSRPAPHWLDSPRDSTTLPDIIASTYSDFIDEAEYATKQMLAILETLNPIEEYIELFESSFKAIEVRLPFDVILNDEKKPDWDAIRRKLIMGSLTVNTTSEKPGITTNQMTAIFDAIPFLFDAVACNPELYEYQNTLSMIVMIAMTGNSMIRRQSINAVQALMERNMLDQDFMRDGVVPGLFNIYQSGVGAFQSNDLRMECVQALCQVISYDAIHDRRWLFDNFLPRFSQMLKDPTMHVRKSALHIFGNLGNMFGQKFTEVFLVPHLITLSCDMTWAVRKVACEIFVKVAECASNQVRIDILSPNFVRLLNDPNKWVSFIAYQQLGPFISLFANPDITGLELRNGQVVVRDPVVLEPSVTEQEDPSNSTFSEDTSDDFRTLPKDTWLPEVDFNDDFEYQLGSIDNTQNQKELTTVTTALVKTPKRGERVMDSVLSGINKMFGSLERSSPKSKEVEKIAATFSSPSKRSPLSLHSAVSLIDKFSKRSNPNNPQRFPVFDSANSDSTLSSNSSNNNSSEQQNSTGIAAKCSSTDDLTKLGLEDDDSPDAAEDSDDFTSETRTTSPRGRRIATNAWSKLDIRQKIFLRRLGALPPSFPKKKATHSSSSSPRKSPTRSPLLSSRKKGLLFDFAAPIEIKSSSSDHSETIPVRTANQQQEKADDDKLNTDERQQYMENSGPKFNTDEDSTNDSDSDDEDNDFEKSFVADEDDGEQDDSPLSIEIKSSSSSDFESVSAATPTSQEPVSNEFSLTYWSSNYAISSIEDELKPFGSSSSFTNSPTSNSYLESRFDVMKVNLSPRARSVSFGTNGSPISTSPRRKANSTSEPSPPNTGNLKSDDSIVMLNTEEKEKLGILDFDEMNMSISSNSSIHGEAAMTEDSDISLTSIEQAALQHVPVDLVESYMRVMGPIGGSGSGGGGNGSSTTIATGDPSLCAETYRHCAHNFPAVCYTLGRAAWPRLKLVFRKLAMDEQARVRQSISHSIHEIANMLGQEITDEDLLPVFYDLRNDQNPDVRNGILIHLYDFVKFLSPEKRDEMILSLPQFFPIGAQPGNQAQNGDWRSRFELISQLSKLCSLYSIQDVNFHMSGIALTLADDRVAEVRREAVMLVSTIVGALVTAEWDNIKGVRDIENGHVDVKKKSNKTDFLSEQFVDDIVSSFAKTQKWTRRQTFCFICAQILRDKQCDGIQFRYFFATPLQQLAQDKVPNVRLASCEALSVWRKTLIAARAQERRGQSPISSPASEPSAIRRDINLVSDMMVKLSNDSDIDAAFIAKKCQGLTDDHQPVDVASRTTRMREREEQFFGDIILSYSPGCNARVSGKEIKQLIRHDQGAVQKAAPLQSMEDFGDEILAHAADSVNIQTPPEVVATVQRVSITNEQFEDNTEAVDMEIEEDEDENSTEKKENTEKTAEEESENVKNTEDELDIPMDPAPTLPPPVTSKNQSSSPITASSSDNNETSA

Target ORF information:

RefSeq Version	NM_001128984.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Serine/threonine-protein phosphatase 4 regulatory subunit 1 (ppfr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128984.3

ORF Insert Sequence:

ATGATAAAGA GCCAATTTGC TACCTTGATC CGTGTGCTGA TCGATTTTCA GAAATTTTGG 
GTTTTTCTCT CAATTTTTTG CACATTTTTA GTGGTTTTCA AGTTCGACAA TCTTATGTTT 
CAAAAAGTTC GGCGAGTTTT CCAACAAAAT CGGAAAAACT CAGAAAGTAT TCAACCCAAG 
TTATCAACAG TTAGCGGATC AAATAGTGGC GAAGGAATGG ATAAAAGAAA TACAGGGTTC 
TCAAATTTGC AACAACTTGG CGGTGCTATC TTTATCGATG ATGCTGAAGA TGAGCCACCA 
AGAGTCAAAA GTTCAGAAGA TTCGAGACCG GCTCCACATT GGCTGGACAG TCCAAGAGAC 
AGCACGACGC TTCCAGATAT CATTGCTTCA ACATATAGTG ATTTCATAGA TGAAGCAGAA 
TATGCGACAA AACAGATGCT GGCCATCTTG GAAACACTGA ATCCTATCGA AGAGTACATT 
GAACTATTTG AGAGCTCATT CAAAGCCATA GAGGTCCGAT TGCCATTCGA TGTGATTTTA 
AATGATGAAA AAAAACCAGA TTGGGATGCG ATTCGGCGAA AACTGATTAT GGGCAGTCTG 
ACAGTGAATA CAACATCTGA AAAGCCAGGA ATAACCACTA ATCAGATGAC AGCGATATTT 
GACGCTATTC CATTTCTTTT TGATGCCGTT GCTTGCAATC CGGAACTTTA TGAATATCAG 
AATACTTTAT CCATGATTGT GATGATTGCG ATGACCGGAA ACTCTATGAT CCGACGACAG 
AGCATCAATG CAGTACAGGC ACTTATGGAA AGGAATATGC TTGATCAGGA TTTCATGAGA 
GATGGCGTTG TTCCCGGACT CTTTAACATT TATCAATCAG GTGTCGGTGC GTTCCAGTCA 
AACGATCTAA GGATGGAGTG TGTCCAGGCA CTTTGCCAGG TTATCTCGTA TGATGCGATC 
CACGATCGAC GATGGTTATT CGATAATTTC CTGCCACGTT TTTCACAAAT GCTCAAAGAT 
CCGACGATGC ATGTCAGGAA GAGTGCCCTT CATATTTTTG GAAATCTTGG AAACATGTTT 
GGACAGAAAT TCACCGAAGT GTTCTTGGTT CCCCACCTGA TTACACTCTC GTGCGATATG 
ACATGGGCGG TTCGGAAAGT TGCATGCGAG ATATTTGTGA AAGTGGCTGA ATGTGCTTCA 
AATCAAGTGA GAATTGACAT TTTGTCACCT AATTTCGTGA GATTATTGAA TGATCCGAAC 
AAATGGGTGT CGTTCATTGC TTATCAACAA CTTGGACCAT TCATTTCACT ATTTGCTAAT 
CCGGATATTA CGGGACTTGA ACTTCGAAAT GGACAAGTCG TTGTCAGAGA TCCAGTAGTA 
TTAGAACCAT CAGTAACTGA ACAGGAAGAT CCATCAAATT CTACATTTTC GGAAGACACA 
TCGGATGATT TTAGAACACT TCCTAAAGAT ACGTGGCTGC CTGAAGTTGA TTTTAATGAT 
GACTTTGAAT ATCAATTGGG ATCAATCGAT AATACACAGA ATCAAAAGGA ATTGACAACT 
GTGACGACAG CATTGGTGAA AACTCCGAAA CGAGGGGAAC GAGTTATGGA CAGCGTTCTG 
TCTGGAATCA ACAAAATGTT TGGATCGTTG GAACGAAGCT CGCCGAAATC AAAAGAAGTC 
GAAAAAATTG CTGCCACATT TTCAAGTCCA TCGAAACGAT CACCACTATC ACTTCACAGT 
GCAGTCAGTC TGATTGATAA ATTCAGCAAA AGAAGCAATC CGAACAATCC ACAGCGATTC 
CCAGTATTTG ATTCTGCCAA CTCGGATTCC ACATTATCAT CTAATTCCTC TAACAATAAC 
AGCTCAGAGC AGCAGAATTC GACGGGAATC GCTGCAAAGT GCAGTTCAAC TGATGATTTG 
ACTAAATTAG GACTTGAAGA CGATGACTCA CCAGATGCAG CTGAAGATTC CGATGATTTC 
ACATCGGAAA CACGGACGAC AAGTCCACGT GGTCGGCGTA TTGCCACCAA CGCGTGGTCG 
AAGCTTGATA TTCGGCAGAA GATATTTCTC CGGCGACTTG GAGCTCTACC ACCGAGCTTT 
CCGAAGAAAA AGGCGACACA CAGCAGCAGC AGCAGTCCGA GAAAATCACC AACAAGATCG 
CCACTGCTGT CGTCGCGAAA AAAGGGACTT TTGTTTGATT TTGCGGCGCC TATTGAAATC 
AAATCATCGT CTTCTGATCA TTCGGAGACA ATTCCAGTCC GGACAGCCAA TCAACAGCAA 
GAAAAAGCAG ATGATGATAA GCTGAATACC GATGAACGGC AGCAATACAT GGAAAACTCT 
GGTCCAAAGT TCAACACTGA TGAAGATTCA ACGAATGATT CAGATAGTGA CGATGAGGAT 
AATGATTTTG AAAAGTCTTT TGTTGCTGAC GAAGATGACG GAGAACAAGA CGATAGTCCA 
TTATCAATTG AAATTAAATC ATCTTCTTCG TCAGACTTTG AATCAGTTTC GGCAGCCACT 
CCAACATCTC AGGAGCCAGT ATCTAATGAG TTTTCATTGA CTTATTGGTC GTCAAACTAT 
GCTATTTCGT CTATCGAAGA TGAGCTCAAA CCTTTTGGAT CCTCATCCTC ATTTACCAAT 
TCTCCAACTT CTAACAGTTA TTTGGAGAGC CGATTCGATG TGATGAAGGT TAATCTTTCG 
CCAAGAGCTC GATCAGTGTC ATTTGGAACT AATGGATCTC CGATATCCAC TTCACCACGA 
AGAAAAGCCA ACAGTACATC TGAACCATCT CCACCAAACA CCGGAAACTT GAAAAGCGAT 
GACTCCATTG TAATGCTCAA TACGGAAGAA AAGGAGAAAC TGGGAATTTT AGATTTCGAT 
GAAATGAACA TGTCAATATC ATCGAATTCA TCGATTCATG GAGAAGCCGC AATGACGGAA 
GATTCGGATA TTAGTCTAAC TTCGATTGAA CAGGCAGCAC TCCAACATGT TCCAGTAGAT 
CTTGTTGAAT CTTATATGAG AGTCATGGGG CCGATTGGAG GTAGTGGAAG CGGAGGTGGT 
GGTAATGGAA GTTCTACAAC CATAGCTACT GGAGATCCAT CGCTTTGCGC GGAGACCTAT 
CGTCATTGTG CTCACAACTT TCCCGCAGTG TGCTATACGC TTGGACGTGC TGCATGGCCT 
CGGCTTAAGC TTGTATTCCG GAAGCTTGCG ATGGATGAGC AGGCTCGAGT TCGTCAGTCG 
ATATCTCATT CGATTCATGA AATAGCAAAT ATGCTTGGAC AGGAGATCAC AGATGAAGAT 
TTGTTGCCAG TTTTCTATGA TTTGAGAAAC GATCAAAATC CAGACGTTCG CAATGGAATA 
TTAATTCATT TGTATGATTT CGTGAAGTTC CTGTCGCCAG AAAAGCGTGA CGAGATGATT 
CTTTCCCTTC CACAATTCTT CCCAATCGGA GCTCAACCAG GAAATCAGGC ACAGAATGGT 
GATTGGAGAT CTAGATTTGA ACTTATATCT CAACTTTCGA AGCTGTGCTC TCTGTACAGT 
ATTCAAGATG TCAATTTTCA CATGTCTGGA ATTGCTCTTA CATTGGCTGA TGATCGTGTC 
GCAGAAGTTC GACGTGAAGC TGTGATGCTT GTTTCAACGA TCGTCGGAGC CCTTGTTACA 
GCTGAATGGG ATAACATCAA AGGAGTTCGG GATATTGAAA ATGGACATGT TGATGTGAAA 
AAGAAGAGTA ACAAAACTGA TTTTTTGTCC GAACAATTTG TCGATGATAT TGTATCCAGT 
TTTGCAAAAA CTCAGAAATG GACAAGACGT CAAACATTCT GCTTCATCTG CGCCCAAATT 
TTACGCGATA AGCAGTGCGA CGGGATTCAG TTCCGATATT TCTTTGCTAC TCCTCTTCAG 
CAGTTGGCTC AAGATAAAGT TCCAAATGTC CGATTGGCAT CTTGCGAGGC GTTAAGTGTG 
TGGAGAAAAA CTTTGATCGC TGCTCGCGCT CAGGAAAGAA GAGGTCAATC TCCGATCTCT 
TCGCCAGCAT CAGAACCATC AGCTATCCGT CGTGACATCA ATCTTGTTTC CGATATGATG 
GTCAAACTCT CCAACGATTC TGATATTGAT GCGGCATTCA TTGCAAAGAA GTGTCAAGGT 
TTAACAGATG ATCATCAGCC AGTTGATGTT GCTTCACGAA CTACACGGAT GCGTGAACGA 
GAAGAACAGT TCTTTGGAGA TATTATTCTT AGCTATTCTC CTGGTTGTAA TGCAAGAGTT 
TCTGGTAAAG AAATTAAACA GTTGATCCGG CATGATCAAG GAGCAGTTCA GAAAGCAGCT 
CCACTTCAAT CAATGGAAGA TTTTGGAGAT GAGATATTGG CTCATGCGGC AGATTCGGTG 
AATATTCAAA CACCTCCGGA AGTAGTTGCT ACTGTTCAAC GAGTCTCAAT TACAAATGAA 
CAATTTGAGG ATAACACTGA AGCGGTTGAT ATGGAAATTG AAGAGGATGA AGATGAAAAC 
TCTACAGAGA AGAAGGAAAA CACTGAAAAA ACCGCAGAGG AAGAATCTGA GAATGTTAAG 
AATACTGAGG ATGAACTGGA TATACCTATG GATCCTGCAC CTACTCTTCC ACCACCAGTG 
ACATCAAAAA ATCAATCTTC ATCACCTATT ACCGCCTCAA GTTCAGATAA CAACGAGACA 
AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ppfr-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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