cle-1 cDNA ORF clone, Caenorhabditis elegans

The following cle-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cle-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa335714 NM_170906.6
Latest version!
Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCa336025 NM_170905.2
Latest version!
Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCa335344 NM_170904.3
Latest version!
Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCa335345 NM_059957.4
Latest version!
Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OCa335344 NM_170904.2 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCa335714 NM_170906.5 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCa336025 NM_170905.1 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCa335345 NM_059957.3 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa335714
    Clone ID Related Accession (Same CDS sequence) NM_170906.5 , NM_170906.6
    Accession Version NM_170906.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170906.5. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
    GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA
    CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC
    CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA
    CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT
    GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC
    AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT
    CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA
    CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT
    CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA
    GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT
    GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA
    CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT
    CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA
    CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT
    GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA
    GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA
    CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT
    GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG
    TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC
    GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG
    CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT
    GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT
    AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG
    CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA
    TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT
    TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT
    GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG
    CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA
    AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA
    GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT
    AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC
    TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740898.1
    CDS1..1953
    Translation

    Target ORF information:

    RefSeq Version NM_170906.6
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170906.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
    GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA
    CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC
    CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA
    CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT
    GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC
    AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT
    CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA
    CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT
    CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA
    GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT
    GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA
    CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT
    CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA
    CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT
    GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA
    GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA
    CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT
    GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG
    TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC
    GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG
    CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT
    GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT
    AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG
    CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA
    TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT
    TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT
    GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG
    CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA
    AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA
    GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT
    AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC
    TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa336025
    Clone ID Related Accession (Same CDS sequence) NM_170905.2 , NM_170905.1
    Accession Version NM_170905.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170905.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
    CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740897.1
    CDS91..2427
    Translation

    Target ORF information:

    RefSeq Version NM_170905.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170905.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
    CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335344
    Clone ID Related Accession (Same CDS sequence) NM_170904.2 , NM_170904.3
    Accession Version NM_170904.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170904.2. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740896.2
    CDS1..3417
    Translation

    Target ORF information:

    RefSeq Version NM_170904.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170904.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335345
    Clone ID Related Accession (Same CDS sequence) NM_059957.4 , NM_059957.3
    Accession Version NM_059957.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059957.3. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT
    CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA
    GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA
    CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA
    GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA
    CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA
    TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC
    CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA
    CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC
    GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA
    GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA
    AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT
    ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT
    GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA
    GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT
    GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC
    TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC
    ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA
    ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA
    ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT
    GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT
    GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT
    GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT
    GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA
    AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC
    GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC
    AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT
    CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC
    GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT
    GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492358.3
    CDS1..3528
    Translation

    Target ORF information:

    RefSeq Version NM_059957.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059957.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT
    CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA
    GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA
    CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA
    GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA
    CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA
    TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC
    CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA
    CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC
    GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA
    GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA
    AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT
    ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT
    GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA
    GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT
    GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC
    TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC
    ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA
    ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA
    ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT
    GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT
    GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT
    GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT
    GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA
    AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC
    GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC
    AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT
    CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC
    GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT
    GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335344
    Clone ID Related Accession (Same CDS sequence) NM_170904.2 , NM_170904.3
    Accession Version NM_170904.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_170904.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740896.2
    CDS1..3417
    Translation

    Target ORF information:

    RefSeq Version NM_170904.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170904.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335345
    Clone ID Related Accession (Same CDS sequence) NM_059957.4 , NM_059957.3
    Accession Version NM_059957.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_059957.2. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT
    CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA
    GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA
    CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA
    GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA
    CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA
    TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC
    CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA
    CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC
    GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA
    GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA
    AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT
    ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT
    GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA
    GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT
    GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC
    TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC
    ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA
    ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA
    ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT
    GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT
    GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT
    GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT
    GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA
    AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC
    GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC
    AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT
    CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC
    GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT
    GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_492358.3
    CDS1..3528
    Translation

    Target ORF information:

    RefSeq Version NM_059957.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059957.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
    TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT
    ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT
    TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT
    GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG
    TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT
    GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT
    CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG
    AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT
    TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT
    TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT
    GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT
    TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT
    CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA
    TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA
    CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT
    CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA
    ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA
    CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT
    GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT
    CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA
    GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA
    CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA
    GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA
    CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA
    TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC
    CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA
    CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC
    GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA
    GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA
    AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT
    ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT
    GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA
    GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT
    GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC
    TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC
    ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA
    ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA
    ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT
    GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT
    GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT
    GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT
    GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA
    AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC
    GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC
    AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT
    CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC
    GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT
    GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa335714
    Clone ID Related Accession (Same CDS sequence) NM_170906.5 , NM_170906.6
    Accession Version NM_170906.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_170906.4. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
    GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA
    CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC
    CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA
    CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT
    GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC
    AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT
    CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA
    CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT
    CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA
    GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT
    GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA
    CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT
    CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA
    CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT
    GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA
    GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA
    CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT
    GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG
    TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC
    GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG
    CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT
    GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT
    AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG
    CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA
    TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT
    TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT
    GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG
    CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA
    AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA
    GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT
    AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC
    TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740898.1
    CDS1..1953
    Translation

    Target ORF information:

    RefSeq Version NM_170906.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170906.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
    GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA
    CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC
    CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA
    CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT
    GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC
    AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT
    CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA
    CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT
    CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA
    GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT
    GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA
    CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT
    CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA
    CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT
    GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA
    GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA
    CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT
    GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG
    TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC
    GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG
    CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT
    GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT
    AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG
    CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA
    TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT
    TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT
    GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG
    CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA
    AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA
    GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT
    AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC
    TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa336025
    Clone ID Related Accession (Same CDS sequence) NM_170905.2 , NM_170905.1
    Accession Version NM_170905.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product CoLlagen with Endostatin domain
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
    CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_740897.1
    CDS1..2337
    Translation

    Target ORF information:

    RefSeq Version NM_170905.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
    CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT
    GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT
    TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT
    GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC
    ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT
    TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA
    CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG
    ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT
    TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT
    TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT
    AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA
    GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA
    TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA
    CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT
    TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC
    GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT
    GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT
    GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT
    GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT
    GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG
    GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA
    GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC
    ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA
    CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG
    TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA
    CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA
    TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA
    AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA
    CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA
    CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT
    CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG
    GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT
    TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT
    TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG
    CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC
    GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA
    AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG
    TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.