cle-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	cle-1
Entrez Gene ID	172678
Full Name	CoLlagen with Endostatin domain
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_170904.2	NP_740896.2	CoLlagen with Endostatin domain
NM_170906.5	NP_740898.1	CoLlagen with Endostatin domain
NM_059957.4	NP_492358.3	CoLlagen with Endostatin domain
NM_170904.3	NP_740896.2	CoLlagen with Endostatin domain
NM_170906.6	NP_740898.1	CoLlagen with Endostatin domain
NM_170905.2	NP_740897.1	CoLlagen with Endostatin domain
NM_170905.1	NP_740897.1	CoLlagen with Endostatin domain
NM_059957.3	NP_492358.3	CoLlagen with Endostatin domain

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	cle-1	NP_740897.1
Caenorhabditis elegans (roundworm)	cle-1	NP_740898.1
Danio rerio (zebrafish)	LOC100334893	XP_002662448.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cle-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cle-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335714	NM_170906.6 Latest version!	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa336025	NM_170905.2 Latest version!	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa335344	NM_170904.3 Latest version!	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa335345	NM_059957.4 Latest version!	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa335344	NM_170904.2	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCa335714	NM_170906.5	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCa336025	NM_170905.1	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCa335345	NM_059957.3	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cle-1 ( NM_170906.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740898.1 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Price & Availability↑

CloneID	OCa335714
Clone ID Related Accession (Same CDS sequence)	NM_170906.5 , NM_170906.6
Accession Version	NM_170906.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170906.5. COMPLETENESS: incomplete on the 5' end.

ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA 
CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC 
CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA 
CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT 
GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC 
AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT 
CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA 
CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT 
CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA 
GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT 
GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA 
CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT 
CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA 
CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT 
GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA 
GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA 
CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT 
GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG 
TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC 
GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG 
CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT 
GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT 
AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG 
CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA 
TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT 
TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT 
GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG 
CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA 
AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA 
GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT 
AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC 
TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740898.1
CDS	1..1953
Translation	MHLLALFIVLFLSPPAVLGGAEVFENDDFPLLSSEQEPIQPEIREFSTSPEDSSNPGTVFQVHVPRDDARVRQARASSEEREFVTQSVMLPVEIVEVIDDETAFEDDGKGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170906.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170906.6

ORF Insert Sequence:

ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA 
CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC 
CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA 
CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT 
GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC 
AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT 
CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA 
CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT 
CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA 
GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT 
GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA 
CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT 
CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA 
CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT 
GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA 
GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA 
CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT 
GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG 
TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC 
GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG 
CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT 
GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT 
AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG 
CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA 
TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT 
TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT 
GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG 
CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA 
AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA 
GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT 
AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC 
TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_170905.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740897.1 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), mRNA.

Price & Availability↑

CloneID	OCa336025
Clone ID Related Accession (Same CDS sequence)	NM_170905.2 , NM_170905.1
Accession Version	NM_170905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170905.1.

ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740897.1
CDS	91..2427
Translation	MLLPRHHLGGLLYFCALYLLRASQAQPPTFFALNALPGDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170905.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170905.2

ORF Insert Sequence:

ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_170904.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740896.2 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Price & Availability↑

CloneID	OCa335344
Clone ID Related Accession (Same CDS sequence)	NM_170904.2 , NM_170904.3
Accession Version	NM_170904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_170904.2. COMPLETENESS: incomplete on the 5' end.

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740896.2
CDS	1..3417
Translation	MIETGSQYLLFLLFICSLFNSGSTALQHEDRVPNAPQNVRIKTQSTSATLWWDAPPDPTVLIRGYTVEYGEGSISQRILIEGPDSTSFTVTRLSPNTNYVFAVSAYNEAEGEDGTKVMVAAKTRPSEGSQTEKLWPPTSVRARIDEKSAAGSAFVSWDDPNPESSSENSIDSTQKQYVINYGIYESDTQQKVRSNAKAVRLTGLIPGKEYEVAVKVVAGDGRESPWSIRDLFLVPETKTVSKFDWFCRLNDTEMCSIHSSPHWKLCSEKHDTYTQRDAGACPRVQYPSSPAHLTTPAINLPDAQRLCLYFRFALLNFHPGQMKVEIFRDGDMANKQIVWKTRMANVRTHVVQNVYVPFSQQIKPFKVSVSLKWDGQPMPRVIVHEMDVLSGGCSERSTDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170904.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170904.3

ORF Insert Sequence:

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_059957.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492358.3 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

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CloneID	OCa335345
Clone ID Related Accession (Same CDS sequence)	NM_059957.4 , NM_059957.3
Accession Version	NM_059957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059957.3. COMPLETENESS: incomplete on the 5' end.

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT 
CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA 
GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA 
CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA 
GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA 
CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA 
TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC 
CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA 
CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC 
GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA 
GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA 
AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT 
ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT 
GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA 
GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT 
GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC 
TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC 
ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA 
ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA 
ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT 
GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT 
GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT 
GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT 
GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA 
AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC 
GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC 
AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT 
CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC 
GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT 
GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492358.3
CDS	1..3528
Translation	MIETGSQYLLFLLFICSLFNSGSTALQHEDRVPNAPQNVRIKTQSTSATLWWDAPPDPTVLIRGYTVEYGEGSISQRILIEGPDSTSFTVTRLSPNTNYVFAVSAYNEAEGEDGTKVMVAAKTRPSEGSQTEKLWPPTSVRARIDEKSAAGSAFVSWDDPNPESSSENSIDSTQKQYVINYGIYESDTQQKVRSNAKAVRLTGLIPGKEYEVAVKVVAGDGRESPWSIRDLFLVPETKTVSKFDWFCRLNDTEMCSIHSSPHWKLCSEKHDTYTQRDAGACPRVQYPSSPAHLTTPAINLPDAQRLCLYFRFALLNFHPGQMKVEIFRDGDMANKQIVWKTRMANVRTHVVQNVYVPFSQQIKPFKVSVSLKWDGQPMPRVIVHEMDVLSGGCSERSTDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWWRRRKIEKELQGRKGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLFLSDDSLVVPFIGGYGSPAARDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_059957.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059957.4

ORF Insert Sequence:

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT 
CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA 
GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA 
CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA 
GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA 
CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA 
TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC 
CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA 
CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC 
GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA 
GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA 
AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT 
ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT 
GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA 
GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT 
GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC 
TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC 
ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA 
ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA 
ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT 
GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT 
GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT 
GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT 
GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA 
AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC 
GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC 
AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT 
CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC 
GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT 
GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_170904.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740896.2 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Price & Availability↑

CloneID	OCa335344
Clone ID Related Accession (Same CDS sequence)	NM_170904.2 , NM_170904.3
Accession Version	NM_170904.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_170904.1. COMPLETENESS: incomplete on both ends.

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740896.2
CDS	1..3417
Translation	MIETGSQYLLFLLFICSLFNSGSTALQHEDRVPNAPQNVRIKTQSTSATLWWDAPPDPTVLIRGYTVEYGEGSISQRILIEGPDSTSFTVTRLSPNTNYVFAVSAYNEAEGEDGTKVMVAAKTRPSEGSQTEKLWPPTSVRARIDEKSAAGSAFVSWDDPNPESSSENSIDSTQKQYVINYGIYESDTQQKVRSNAKAVRLTGLIPGKEYEVAVKVVAGDGRESPWSIRDLFLVPETKTVSKFDWFCRLNDTEMCSIHSSPHWKLCSEKHDTYTQRDAGACPRVQYPSSPAHLTTPAINLPDAQRLCLYFRFALLNFHPGQMKVEIFRDGDMANKQIVWKTRMANVRTHVVQNVYVPFSQQIKPFKVSVSLKWDGQPMPRVIVHEMDVLSGGCSERSTDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170904.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170904.2

ORF Insert Sequence:

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_059957.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492358.3 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Price & Availability↑

CloneID	OCa335345
Clone ID Related Accession (Same CDS sequence)	NM_059957.4 , NM_059957.3
Accession Version	NM_059957.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_059957.2. COMPLETENESS: incomplete on both ends.

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT 
CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA 
GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA 
CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA 
GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA 
CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA 
TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC 
CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA 
CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC 
GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA 
GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA 
AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT 
ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT 
GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA 
GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT 
GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC 
TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC 
ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA 
ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA 
ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT 
GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT 
GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT 
GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT 
GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA 
AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC 
GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC 
AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT 
CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC 
GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT 
GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492358.3
CDS	1..3528
Translation	MIETGSQYLLFLLFICSLFNSGSTALQHEDRVPNAPQNVRIKTQSTSATLWWDAPPDPTVLIRGYTVEYGEGSISQRILIEGPDSTSFTVTRLSPNTNYVFAVSAYNEAEGEDGTKVMVAAKTRPSEGSQTEKLWPPTSVRARIDEKSAAGSAFVSWDDPNPESSSENSIDSTQKQYVINYGIYESDTQQKVRSNAKAVRLTGLIPGKEYEVAVKVVAGDGRESPWSIRDLFLVPETKTVSKFDWFCRLNDTEMCSIHSSPHWKLCSEKHDTYTQRDAGACPRVQYPSSPAHLTTPAINLPDAQRLCLYFRFALLNFHPGQMKVEIFRDGDMANKQIVWKTRMANVRTHVVQNVYVPFSQQIKPFKVSVSLKWDGQPMPRVIVHEMDVLSGGCSERSTDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWWRRRKIEKELQGRKGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLFLSDDSLVVPFIGGYGSPAARDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_059957.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059957.3

ORF Insert Sequence:

ATGATAGAAA CTGGATCTCA ATATTTATTA TTTCTACTAT TTATTTGTTC TTTATTTAAT 
TCTGGTTCAA CAGCATTACA ACATGAGGAT CGTGTTCCAA ATGCACCACA AAACGTTCGT 
ATCAAGACCC AATCCACATC AGCCACCTTA TGGTGGGATG CACCTCCGGA TCCTACAGTT 
TTAATTAGAG GATATACTGT AGAGTATGGA GAAGGAAGTA TTTCACAGAG AATTCTTATT 
GAAGGACCAG ATAGCACTTC ATTTACTGTT ACTAGACTTT CGCCAAATAC AAATTACGTG 
TTTGCTGTTT CTGCGTACAA TGAAGCAGAG GGAGAGGATG GTACAAAAGT TATGGTTGCT 
GCAAAAACTA GGCCATCAGA AGGAAGTCAA ACAGAAAAAC TATGGCCTCC GACTTCTGTT 
CGTGCAAGAA TCGATGAAAA ATCAGCCGCT GGAAGTGCAT TTGTATCATG GGACGATCCG 
AATCCGGAGA GCTCATCTGA AAATTCAATT GATTCGACAC AAAAACAATA TGTTATTAAT 
TATGGAATAT ACGAAAGTGA TACGCAACAA AAGGTACGTT CAAATGCGAA AGCAGTTCGT 
TTAACCGGGT TAATACCTGG CAAAGAATAT GAAGTAGCTG TAAAAGTGGT GGCTGGGGAT 
GGAAGGGAAA GTCCATGGAG TATTAGAGAT TTGTTTTTAG TTCCCGAGAC GAAAACAGTT 
TCGAAGTTTG ATTGGTTCTG TCGTCTTAAT GACACTGAAA TGTGCTCAAT CCATAGCTCT 
CCTCACTGGA AACTTTGCTC TGAAAAACAT GATACTTACA CTCAACGTGA CGCAGGAGCA 
TGCCCACGAG TACAGTATCC ATCATCACCT GCACATCTAA CAACACCAGC TATCAATCTA 
CCAGATGCTC AACGATTGTG TTTATACTTC AGATTTGCAT TACTCAACTT TCATCCCGGT 
CAAATGAAAG TAGAAATCTT TCGTGATGGA GATATGGCCA ATAAACAAAT TGTTTGGAAA 
ACTCGAATGG CAAATGTTCG AACTCATGTT GTTCAAAACG TCTATGTTCC ATTTTCTCAA 
CAGATAAAGC CATTCAAGGT ATCAGTTTCT CTCAAATGGG ATGGTCAACC AATGCCACGT 
GTAATTGTTC ATGAAATGGA TGTACTGAGT GGGGGTTGTT CAGAAAGATC AACTGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GTGGCGTCGT 
CGAAAGATTG AGAAAGAGCT TCAGGGTCGA AAAGGTGCTG AAGGTTCGGG TACTCCAGAA 
GAATTCAAGA AAACTCAAGA ATCATCGGAA CTTCAAAAGA TATCCGATTT TCCACCGACA 
CCTGCTCCCC CACCCCCATA CCCAACACCT CAGTTGGCTC CACATGACTT GTTTCTATCA 
GATGATTCTC TTGTTGTTCC ATTTATCGGT GGTTACGGTA GCCCCGCAGC TAGAGATCTA 
CGCTCATACG AACAACAAGC TGCGACAACT CCATTCCAAG GTGCCCCACC TTCCAATCAA 
TGCACTCAAG TTTGTCGTGG AGAACCGGGA CCTCAAGGTC CATCAGGAAA CGATGGAATC 
CCTGGATCAC ACGGAGCTCC GGGTCATCAA GGAGAAAGAG GAGCTGATGG TGCTCCAGGA 
CTTCATGGAT CCCGTGGTGA TCAGGGGCTG CCAGGTCCAC CTGGTATGCC TGGCCTTGCC 
GGACCACCTG GACCACCTGG TTCAGGTACT GGACATGGAT CTGGAGCTGA TGGGCCACAA 
GGACCACCGG GACTTCCTGG AGCTCCTGGA AGAGATGGAA CATCGGGAGT TGAAGGACAA 
AGAGGACCTC AAGGACCACC TGGACCAAAA GGAGATGATG GTGTTAGTAT GGAAAGTCTT 
ACTGATGATG ATATTGAAAG AATTGCAAGA AGAGTAGCAT CAATTCAAAA AGAATCAAAT 
GAACCAATGA GAGGAGACCT CCCAGAATAC AATTCAAAAG TTCATAGTTT CACAACAAAA 
GGAGAAAAGG GAGAACGAGG CGCGCCTGGT GCCCCGGGAG CACCAGCACC ACCAGCTTAT 
GGAACACTCA CCACTGGAAC TGCAGTAAAT GTTCATGCAA CCACGGTGGA GTTATTCGCC 
TCAGCCCGTT CTACGTCAGT TGGGCAACTG GCATTTGCCA CGTCATCTCA ACAATTATTC 
ATTCGTGTTA CAAACGGATG GAAAGAAATT CAATTAACCC ATTTCCATCC ATTTGTAGAA 
ACAAGACATT CAACGCAAAA CTCTCGACAA AACGATGCAA GTCACGCGGG AAGATCAAGA 
ACTGGATCAG CAGCTGCTCC ATGGTACCCA AAAGCAAATC TTGATGAACC ACAGAGAGAT 
GCGGGAGTTC ATAATAAAGA TCGAGTTATT CATATGATTG CCCTGTCACA ACCATTCTCT 
GGTAATCTTC ACGGGCTCCG AGGAGCAGAT CTTCAATGTT ATCGTGAAGC TCGTGCAGCT 
GGATATACTA CAACATTCCG TGCAATGCTT TCGAGTAATG TACAGGATTT GGTTAGAATT 
GTGCATTCCG TTGATTTTGA CACAACTGTT GTGAATGTTG CTGGACATCA TTTGTTCCCA 
AGTTGGAGAT CATTTGTCAA TGGAGCACAA ATGAATCCTC ATGCCAAACT TTTCTCATTC 
GATAGACATG ATGTGCTCAA CGATTCTAGA TGGCCAGATA AGCGAGTTTG GCATGGATCC 
AAAGATGGTG GAATAAGAGC TGAACAATAC TGTGATGGAT GGAGAAGAGC CGATTCATCT 
CTGACTTCTT TGGCAGGTCA CATCTCATCG AATACAAGCA TTTTCCAATC AAGTGGATCC 
GAAAAATGTG AAAATAAATT AGTGGTATTG TGTGTTGAGA ACATGTCCAA GTATCACGGT 
GACCGAATTC TCCGCTTGCA TCGAATCACA TCCGACTTCA AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_170906.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740898.1 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

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CloneID	OCa335714
Clone ID Related Accession (Same CDS sequence)	NM_170906.5 , NM_170906.6
Accession Version	NM_170906.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_170906.4. COMPLETENESS: incomplete on both ends.

ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA 
CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC 
CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA 
CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT 
GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC 
AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT 
CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA 
CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT 
CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA 
GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT 
GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA 
CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT 
CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA 
CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT 
GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA 
GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA 
CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT 
GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG 
TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC 
GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG 
CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT 
GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT 
AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG 
CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA 
TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT 
TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT 
GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG 
CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA 
AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA 
GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT 
AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC 
TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740898.1
CDS	1..1953
Translation	MHLLALFIVLFLSPPAVLGGAEVFENDDFPLLSSEQEPIQPEIREFSTSPEDSSNPGTVFQVHVPRDDARVRQARASSEEREFVTQSVMLPVEIVEVIDDETAFEDDGKGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170906.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170906.5

ORF Insert Sequence:

ATGCATCTCC TTGCACTATT TATAGTGCTC TTTCTCTCAC CACCGGCCGT TCTTGGCGGA 
GCAGAAGTGT TTGAAAATGA TGATTTTCCA CTTTTGTCAT CCGAACAAGA GCCCATTCAA 
CCGGAAATTC GTGAATTCTC GACGTCTCCA GAAGACTCAT CAAATCCTGG TACCGTTTTC 
CAAGTTCATG TACCACGTGA CGATGCACGT GTCAGACAGG CACGAGCATC TTCTGAAGAA 
CGTGAATTTG TGACACAGAG TGTGATGTTA CCTGTGGAAA TTGTGGAAGT GATCGATGAT 
GAGACTGCAT TTGAAGATGA TGGAAAGGGT GCTGAAGGTT CGGGTACTCC AGAAGAATTC 
AAGAAAACTC AAGAATCATC GGAACTTCAA AAGATATCCG ATTTTCCACC GACACCTGCT 
CCCCCACCCC CATACCCAAC ACCTCAGTTG GCTCCACATG ATCTACGCTC ATACGAACAA 
CAAGCTGCGA CAACTCCATT CCAAGGTGCC CCACCTTCCA ATCAATGCAC TCAAGTTTGT 
CGTGGAGAAC CGGGACCTCA AGGTCCATCA GGAAACGATG GAATCCCTGG ATCACACGGA 
GCTCCGGGTC ATCAAGGAGA AAGAGGAGCT GATGGTGCTC CAGGACTTCA TGGATCCCGT 
GGTGATCAGG GGCTGCCAGG TCCACCTGGT ATGCCTGGCC TTGCCGGACC ACCTGGACCA 
CCTGGTTCAG GTACTGGACA TGGATCTGGA GCTGATGGGC CACAAGGACC ACCGGGACTT 
CCTGGAGCTC CTGGAAGAGA TGGAACATCG GGAGTTGAAG GACAAAGAGG ACCTCAAGGA 
CCACCTGGAC CAAAAGGAGA TGATGGTGTT AGTATGGAAA GTCTTACTGA TGATGATATT 
GAAAGAATTG CAAGAAGAGT AGCATCAATT CAAAAAGAAT CAAATGAACC AATGAGAGGA 
GACCTCCCAG AATACAATTC AAAAGTTCAT AGTTTCACAA CAAAAGGAGA AAAGGGAGAA 
CGAGGCGCGC CTGGTGCCCC GGGAGCACCA GCACCACCAG CTTATGGAAC ACTCACCACT 
GGAACTGCAG TAAATGTTCA TGCAACCACG GTGGAGTTAT TCGCCTCAGC CCGTTCTACG 
TCAGTTGGGC AACTGGCATT TGCCACGTCA TCTCAACAAT TATTCATTCG TGTTACAAAC 
GGATGGAAAG AAATTCAATT AACCCATTTC CATCCATTTG TAGAAACAAG ACATTCAACG 
CAAAACTCTC GACAAAACGA TGCAAGTCAC GCGGGAAGAT CAAGAACTGG ATCAGCAGCT 
GCTCCATGGT ACCCAAAAGC AAATCTTGAT GAACCACAGA GAGATGCGGG AGTTCATAAT 
AAAGATCGAG TTATTCATAT GATTGCCCTG TCACAACCAT TCTCTGGTAA TCTTCACGGG 
CTCCGAGGAG CAGATCTTCA ATGTTATCGT GAAGCTCGTG CAGCTGGATA TACTACAACA 
TTCCGTGCAA TGCTTTCGAG TAATGTACAG GATTTGGTTA GAATTGTGCA TTCCGTTGAT 
TTTGACACAA CTGTTGTGAA TGTTGCTGGA CATCATTTGT TCCCAAGTTG GAGATCATTT 
GTCAATGGAG CACAAATGAA TCCTCATGCC AAACTTTTCT CATTCGATAG ACATGATGTG 
CTCAACGATT CTAGATGGCC AGATAAGCGA GTTTGGCATG GATCCAAAGA TGGTGGAATA 
AGAGCTGAAC AATACTGTGA TGGATGGAGA AGAGCCGATT CATCTCTGAC TTCTTTGGCA 
GGTCACATCT CATCGAATAC AAGCATTTTC CAATCAAGTG GATCCGAAAA ATGTGAAAAT 
AAATTAGTGG TATTGTGTGT TGAGAACATG TCCAAGTATC ACGGTGACCG AATTCTCCGC 
TTGCATCGAA TCACATCCGA CTTCAAAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cle-1 ( NM_170905.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_740897.1 Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Price & Availability↑

CloneID	OCa336025
Clone ID Related Accession (Same CDS sequence)	NM_170905.2 , NM_170905.1
Accession Version	NM_170905.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	CoLlagen with Endostatin domain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740897.1
CDS	1..2337
Translation	MLLPRHHLGGLLYFCALYLLRASQAQPPTFFALNALPGDSETEVDLLAPVKASLNADARVFRAKGIESLPAIGLQRGVEIAVPYRLYLPRNFFKQFSLLATIKPMDKRGGYLFAAVNAYDSAVDIGLLIEPAGTKQTNISLIVRSVAIVSFLVEDFSQQWTQFALEVVDQTVTFYFKCRRFASRQVTSLPDFSFDEAEKLYIASAGPIIDNGFEGAIQELKLIDDATQGSRQCDEQWGAEGSGTPEEFKKTQESSELQKISDFPPTPAPPPPYPTPQLAPHDLRSYEQQAATTPFQGAPPSNQCTQVCRGEPGPQGPSGNDGIPGSHGAPGHQGERGADGAPGLHGSRGDQGLPGPPGMPGLAGPPGPPGSGTGHGSGADGPQGPPGLPGAPGRDGTSGVEGQRGPQGPPGPKGDDGVSMESLTDDDIERIARRVASIQKESNEPMRGDLPEYNSKVHSFTTKGEKGERGAPGAPGAPAPPAYGTLTTGTAVNVHATTVELFASARSTSVGQLAFATSSQQLFIRVTNGWKEIQLTHFHPFVETRHSTQNSRQNDASHAGRSRTGSAAAPWYPKANLDEPQRDAGVHNKDRVIHMIALSQPFSGNLHGLRGADLQCYREARAAGYTTTFRAMLSSNVQDLVRIVHSVDFDTTVVNVAGHHLFPSWRSFVNGAQMNPHAKLFSFDRHDVLNDSRWPDKRVWHGSKDGGIRAEQYCDGWRRADSSLTSLAGHISSNTSIFQSSGSEKCENKLVVLCVENMSKYHGDRILRLHRITSDFKK

Target ORF information:

RefSeq Version	NM_170905.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CoLlagen with Endostatin domain (cle-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170905.1

ORF Insert Sequence:

ATGTTGTTGC CACGTCATCA TCTTGGAGGG TTACTGTACT TTTGTGCTCT ATATTTATTA 
CGAGCATCAC AAGCACAGCC ACCTACATTC TTTGCTCTTA ACGCGCTTCC AGGAGATTCT 
GAAACGGAAG TAGATTTATT GGCTCCAGTT AAAGCATCTT TAAATGCAGA CGCACGGGTT 
TTTAGAGCCA AAGGAATTGA ATCTTTGCCA GCAATCGGGT TACAGAGAGG TGTTGAAATT 
GCAGTCCCTT ATCGATTATA TTTGCCACGA AATTTTTTCA AACAATTTTC TCTACTTGCC 
ACTATCAAGC CAATGGATAA ACGAGGTGGT TATCTATTTG CTGCAGTTAA TGCATATGAT 
TCAGCAGTTG ATATTGGATT GCTTATTGAA CCAGCAGGAA CAAAGCAAAC GAATATTTCA 
CTGATAGTTC GTTCTGTGGC AATTGTATCG TTTCTTGTCG AAGATTTCTC TCAGCAATGG 
ACACAATTTG CATTAGAAGT TGTAGATCAA ACTGTCACAT TTTACTTCAA ATGCAGACGT 
TTTGCATCAA GACAGGTTAC CTCTCTCCCT GATTTCTCAT TTGATGAAGC AGAGAAGCTT 
TATATTGCAA GTGCAGGACC AATCATCGAT AACGGATTTG AGGGAGCTAT TCAAGAACTT 
AAATTAATTG ATGATGCTAC ACAAGGTTCA CGACAGTGTG ATGAACAATG GGGTGCTGAA 
GGTTCGGGTA CTCCAGAAGA ATTCAAGAAA ACTCAAGAAT CATCGGAACT TCAAAAGATA 
TCCGATTTTC CACCGACACC TGCTCCCCCA CCCCCATACC CAACACCTCA GTTGGCTCCA 
CATGATCTAC GCTCATACGA ACAACAAGCT GCGACAACTC CATTCCAAGG TGCCCCACCT 
TCCAATCAAT GCACTCAAGT TTGTCGTGGA GAACCGGGAC CTCAAGGTCC ATCAGGAAAC 
GATGGAATCC CTGGATCACA CGGAGCTCCG GGTCATCAAG GAGAAAGAGG AGCTGATGGT 
GCTCCAGGAC TTCATGGATC CCGTGGTGAT CAGGGGCTGC CAGGTCCACC TGGTATGCCT 
GGCCTTGCCG GACCACCTGG ACCACCTGGT TCAGGTACTG GACATGGATC TGGAGCTGAT 
GGGCCACAAG GACCACCGGG ACTTCCTGGA GCTCCTGGAA GAGATGGAAC ATCGGGAGTT 
GAAGGACAAA GAGGACCTCA AGGACCACCT GGACCAAAAG GAGATGATGG TGTTAGTATG 
GAAAGTCTTA CTGATGATGA TATTGAAAGA ATTGCAAGAA GAGTAGCATC AATTCAAAAA 
GAATCAAATG AACCAATGAG AGGAGACCTC CCAGAATACA ATTCAAAAGT TCATAGTTTC 
ACAACAAAAG GAGAAAAGGG AGAACGAGGC GCGCCTGGTG CCCCGGGAGC ACCAGCACCA 
CCAGCTTATG GAACACTCAC CACTGGAACT GCAGTAAATG TTCATGCAAC CACGGTGGAG 
TTATTCGCCT CAGCCCGTTC TACGTCAGTT GGGCAACTGG CATTTGCCAC GTCATCTCAA 
CAATTATTCA TTCGTGTTAC AAACGGATGG AAAGAAATTC AATTAACCCA TTTCCATCCA 
TTTGTAGAAA CAAGACATTC AACGCAAAAC TCTCGACAAA ACGATGCAAG TCACGCGGGA 
AGATCAAGAA CTGGATCAGC AGCTGCTCCA TGGTACCCAA AAGCAAATCT TGATGAACCA 
CAGAGAGATG CGGGAGTTCA TAATAAAGAT CGAGTTATTC ATATGATTGC CCTGTCACAA 
CCATTCTCTG GTAATCTTCA CGGGCTCCGA GGAGCAGATC TTCAATGTTA TCGTGAAGCT 
CGTGCAGCTG GATATACTAC AACATTCCGT GCAATGCTTT CGAGTAATGT ACAGGATTTG 
GTTAGAATTG TGCATTCCGT TGATTTTGAC ACAACTGTTG TGAATGTTGC TGGACATCAT 
TTGTTCCCAA GTTGGAGATC ATTTGTCAAT GGAGCACAAA TGAATCCTCA TGCCAAACTT 
TTCTCATTCG ATAGACATGA TGTGCTCAAC GATTCTAGAT GGCCAGATAA GCGAGTTTGG 
CATGGATCCA AAGATGGTGG AATAAGAGCT GAACAATACT GTGATGGATG GAGAAGAGCC 
GATTCATCTC TGACTTCTTT GGCAGGTCAC ATCTCATCGA ATACAAGCAT TTTCCAATCA 
AGTGGATCCG AAAAATGTGA AAATAAATTA GTGGTATTGT GTGTTGAGAA CATGTCCAAG 
TATCACGGTG ACCGAATTCT CCGCTTGCAT CGAATCACAT CCGACTTCAA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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cle-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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