crml-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	crml-1
Entrez Gene ID	172455
Full Name	CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001322686.1	NP_001309450.1	CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog
NM_001322686.2	NP_001309450.1	Carm_PH domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UNC-73, the C. elegans ortholog of Trio, is a guanine nucleotide exchange factor (GEF) upstream of both CED-10 and MIG-2 that is suppressed by CMRL1
Title: Polarized Rac-dependent protrusions drive epithelial intercalation in the embryonic epidermis of C. elegans.

propose a role for CRML-1 as a novel regulator of cell and axon migrations that acts through inhibition of Rac signaling
Title: C. elegans CARMIL negatively regulates UNC-73/Trio function during neuronal development.

The following crml-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crml-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335267	NM_001322686.2 Latest version!	Caenorhabditis elegans Carm_PH domain-containing protein (crml-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa335267	NM_001322686.1	Caenorhabditis elegans CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog (crml-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crml-1 ( NM_001322686.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001309450.1 Caenorhabditis elegans CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog (crml-1), partial mRNA.

Price & Availability↑

CloneID	OCa335267
Clone ID Related Accession (Same CDS sequence)	NM_001322686.1 , NM_001322686.2
Accession Version	NM_001322686.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGTCCATGT CGAGGAGCAC CATCCAGGAA TTGTGTGATT TCGTACAACA TAAACCATCA 
CAAATCCTAG GAAACAAATT CTTCGACTCG CGGATTGTGG TTCAGGTGGA TGTGGCACAC 
AAAGCAGATA AATATGAGCC ACGTATTTTT ATCATATCGA AATTTAGAAT ATTCTTTCTT 
ATGGGTAAAA CTACATCGTC ATTGAAAATC GAAAAATCAT TTCATGTGCT GGCGATAAAA 
GCGATTCATG TGATAAGTGA AGAAGAATTG GTGATTCATT TGGATGATGG TGTACATAAA 
AAGAAGATTA ATATTAAATG TTCAATTCAT CCAGCTGTTC ATTTGGCTAG ACAACTGTTA 
TCAGCTGTCA AACACTATTT TCCAGATTTC GGAGTTTATC TACAATCATC AATCGATGTG 
AATCCATCTG CTCTCTACAC AGAATTTCCA TCTCTTCCTA CAGCTTCTCC ACGCCCATGT 
CATTCTTTTC GGCGAACTTA CGCGGCTTTG TGTGATTTCT ATGATCAACC TTATCGAGAT 
GAAGTATCGT GGGATGTCGA GAAAATATAC ACAGCAAACA AATTACGAGA TTTGAAAATT 
GACGACTTCT CGCATCTCCT TCCAAAAGAT CTTCTTCCAA TCGTTGGCGT CCTTCAATAT 
TCAGCATATT TTACGGGACT TATCTGTGAT GCAGTCCGAG TATCCTCCGA AGTTATCGAT 
GTGGTGTTGA GTGTAATCCG AAAGTCACAA AATCTGAAAA AGCTTCAACT GAGGAGTTGC 
AGTCTCCCAA AAGATTTCAT TACTCTCCTT GCATCAGCTT TTCACAATAA CCAAAATGCC 
TGCCTCGAGT ATTTGGATCT TTCAAGGAAT CCGATTGATG ACAAAAAAGG ATTCACAATT 
TTGTCCTCCG TGCTTCCTCG GCTCAATCAG CTAAAATATG TCAACTTTTC TGAATGTCAA 
TTATCGGATA AATCTATTAA TTTGTTATGC ACTGGTCTAT ATAATGGAAT GACGTCATGC 
AAAAGTGGAG GAATGCAACT AACAGAGCTC ATTTTGTCAG CAAATCCTAT GAAGGATGAT 
ATATCTGGAA TCATCAATCT GGTTTCAATT TCCACTTCGC TCCGAGTTCT CGACTTTTCT 
GATACTGGTA TACATCTGGA CAAACTCTGG AATTCTTTAA AATTCGGTGG CCTTCAAATC 
GAAAAGCTAA TATTGAATGG TTGCTCATTG AGCAAAAAAT CAGAAGGAGT TCAAACCGCG 
AAGGAATATT TTTCGATGGC AGTCAATCTA TCGCATATTT CATTCAACAA CACGTCAATG 
CCATCTGAAT ATCTTAAAGC AGTTCTTCTT GGACTTGCTT CGAATCAACA ACTTCAACCA 
TTCCGTCTGG ATTTAGATGC GACGTGTGAG AAAGGATCCG CAAGTGTACT TGATGCATGC 
ATTGGAGGAA TTCGTTGTGA GACCCTCAGT CTTCGAGATA ATAATCTGGA TGGTGAACTG 
CAAGGAGTTT TGCAGAGTCT GATGATGGTT ACATGTCTAC GAAGACTTGA TATTGGAGGA 
TCTAATATGA ATCAATTGAA GAAAAACAAC AAACAAGTGC ATGTGATCAA TAAAGTTCTT 
CTGGATGTAG TAAAGCTATA CAGCGAGGAA GGGTGTCTCG AAGAGCTCAA TCTCTCGGAT 
TGCCGTCTCG GTGCATATCT AAGCGTACTT ATCAACACAT TGGCAGCAAC TACAACGCTC 
AAAAGTCTTG ATATAAGTGG AAATGAGATG GGAAACTTTG GAGCCAGAAT TCTTTCGAAA 
GCACTACAAG TTAATGTATC TCTTCGATCT GTGTCCATTG ACAATAATCA TATTGGTGCT 
GATGGTTTTG TCGATTTGGC AACGTCTATC AAGATGAACC ACACATTAAC TCATTTCCCT 
TATCCTGTTC ATGATGCATT TGACTGTATG CAGCGTGTAG AACGACCGAG GACAGTGGCA 
GCATTATCAC AGATTCAAAA CTGCTTGTAT CGGAATAGAA CAGCTCTCGG AGCCGACGAA 
GCCAATTGCA AGAAAATGTT CGCAGAGGGA GTGCTACAGA AAATGGAGAA AGCGAATGAT 
GAGGTTGTTG GAAGCATTGT TGACTCAATT TCAGCTTTTG GACAAGAACC ATTTCCACCA 
AAAATGATTG AAATCATTGA AGAATTCGTT GGAAAGTTTC AAATGGAAGG CGGTCGGATC 
ATTTCTGAGA GCATTTCAAG AACAATCGGA GGTGATTTAA GTGCAACATC GCGTCGTGCA 
GAGCAACTTG CAACAGCTAG ACTTGCTGAA GTTTGCACAG AACAAGTGAA ACATGTGTTT 
AGTGAATGGA AATGGAGGGA AATGTGCGAA CACGTGGAAT CTGAACTGTC CAAAATGTCA 
ATTGGAGGAT CTGGTGATGC ATGCAGCCTT GGAAGAACGT CATCTGTGGG GACAGGATCA 
CCTGCAATGT CATCTCCATT CACTCCGAAA CGTTCTCAGG CAGGACATCG TCCGAGAAGT 
ATAATTGGAG ATCTGACATC ATCGACAACA TCTTCCGAAA CAGTTGGAGA TTCTACGGAG 
CAAGGAGTCA ATTTAGATTT GCCACCAAAG CCTTCAGTCC TATCACATCT ACAAAAGGCA 
AGACCAAGGA AAAGAGGAGG AGCTTCATCT ATTCTAAATT CATCCCTTTC TGAAGACGTG 
ATGATGACAA GTAAATCAAG TGGAGGACCA GAGAATGGAG ATTTGGCTGA TCTAATTGAA 
GAAGTTGATG AAGTTGAACA AGAACCAAGA AAAGTTCCAA CTGGTGTTCG TGTTGCTATG 
ATACCAGATA GTTCCCTCAT TTCACAAGTG CATCTTCGTC CTTCAGCTGA ACGTACTCTT 
TCTGCTTCTT CTGACTTCTC ACTGTCTCCA CGTGAAGCTT CTTCTCCAAC AACAAGTAAC 
AGTAAGATCA TGACGTCATC AAGTGCTTCG GATTCTCCAC CACCTTTGCC TCAACGTAAT 
AGAGTTGGAC CTGGCGGTGC TCCACCCCCA TTACTTCCTC CTAAACCAGA ACCTCGAAGT 
CGTTTGATTG GAATGACGTC TCCAACTACA CCTCCACCTA CAGCAGATGA TGATGAAAAT 
GCAAATAGTC GTCGCTCTGT GGCTGATATG GCTAGAATTT TCCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309450.1
CDS	1..3171
Translation	MSMSRSTIQELCDFVQHKPSQILGNKFFDSRIVVQVDVAHKADKYEPRIFIISKFRIFFLMGKTTSSLKIEKSFHVLAIKAIHVISEEELVIHLDDGVHKKKINIKCSIHPAVHLARQLLSAVKHYFPDFGVYLQSSIDVNPSALYTEFPSLPTASPRPCHSFRRTYAALCDFYDQPYRDEVSWDVEKIYTANKLRDLKIDDFSHLLPKDLLPIVGVLQYSAYFTGLICDAVRVSSEVIDVVLSVIRKSQNLKKLQLRSCSLPKDFITLLASAFHNNQNACLEYLDLSRNPIDDKKGFTILSSVLPRLNQLKYVNFSECQLSDKSINLLCTGLYNGMTSCKSGGMQLTELILSANPMKDDISGIINLVSISTSLRVLDFSDTGIHLDKLWNSLKFGGLQIEKLILNGCSLSKKSEGVQTAKEYFSMAVNLSHISFNNTSMPSEYLKAVLLGLASNQQLQPFRLDLDATCEKGSASVLDACIGGIRCETLSLRDNNLDGELQGVLQSLMMVTCLRRLDIGGSNMNQLKKNNKQVHVINKVLLDVVKLYSEEGCLEELNLSDCRLGAYLSVLINTLAATTTLKSLDISGNEMGNFGARILSKALQVNVSLRSVSIDNNHIGADGFVDLATSIKMNHTLTHFPYPVHDAFDCMQRVERPRTVAALSQIQNCLYRNRTALGADEANCKKMFAEGVLQKMEKANDEVVGSIVDSISAFGQEPFPPKMIEIIEEFVGKFQMEGGRIISESISRTIGGDLSATSRRAEQLATARLAEVCTEQVKHVFSEWKWREMCEHVESELSKMSIGGSGDACSLGRTSSVGTGSPAMSSPFTPKRSQAGHRPRSIIGDLTSSTTSSETVGDSTEQGVNLDLPPKPSVLSHLQKARPRKRGGASSILNSSLSEDVMMTSKSSGGPENGDLADLIEEVDEVEQEPRKVPTGVRVAMIPDSSLISQVHLRPSAERTLSASSDFSLSPREASSPTTSNSKIMTSSSASDSPPPLPQRNRVGPGGAPPPLLPPKPEPRSRLIGMTSPTTPPPTADDDENANSRRSVADMARIFQK

Target ORF information:

RefSeq Version	NM_001322686.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans CARMIL (Capping, ARp2/3, Myosin I Linker protein) homolog (crml-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322686.1

ORF Insert Sequence:

ATGTCCATGT CGAGGAGCAC CATCCAGGAA TTGTGTGATT TCGTACAACA TAAACCATCA 
CAAATCCTAG GAAACAAATT CTTCGACTCG CGGATTGTGG TTCAGGTGGA TGTGGCACAC 
AAAGCAGATA AATATGAGCC ACGTATTTTT ATCATATCGA AATTTAGAAT ATTCTTTCTT 
ATGGGTAAAA CTACATCGTC ATTGAAAATC GAAAAATCAT TTCATGTGCT GGCGATAAAA 
GCGATTCATG TGATAAGTGA AGAAGAATTG GTGATTCATT TGGATGATGG TGTACATAAA 
AAGAAGATTA ATATTAAATG TTCAATTCAT CCAGCTGTTC ATTTGGCTAG ACAACTGTTA 
TCAGCTGTCA AACACTATTT TCCAGATTTC GGAGTTTATC TACAATCATC AATCGATGTG 
AATCCATCTG CTCTCTACAC AGAATTTCCA TCTCTTCCTA CAGCTTCTCC ACGCCCATGT 
CATTCTTTTC GGCGAACTTA CGCGGCTTTG TGTGATTTCT ATGATCAACC TTATCGAGAT 
GAAGTATCGT GGGATGTCGA GAAAATATAC ACAGCAAACA AATTACGAGA TTTGAAAATT 
GACGACTTCT CGCATCTCCT TCCAAAAGAT CTTCTTCCAA TCGTTGGCGT CCTTCAATAT 
TCAGCATATT TTACGGGACT TATCTGTGAT GCAGTCCGAG TATCCTCCGA AGTTATCGAT 
GTGGTGTTGA GTGTAATCCG AAAGTCACAA AATCTGAAAA AGCTTCAACT GAGGAGTTGC 
AGTCTCCCAA AAGATTTCAT TACTCTCCTT GCATCAGCTT TTCACAATAA CCAAAATGCC 
TGCCTCGAGT ATTTGGATCT TTCAAGGAAT CCGATTGATG ACAAAAAAGG ATTCACAATT 
TTGTCCTCCG TGCTTCCTCG GCTCAATCAG CTAAAATATG TCAACTTTTC TGAATGTCAA 
TTATCGGATA AATCTATTAA TTTGTTATGC ACTGGTCTAT ATAATGGAAT GACGTCATGC 
AAAAGTGGAG GAATGCAACT AACAGAGCTC ATTTTGTCAG CAAATCCTAT GAAGGATGAT 
ATATCTGGAA TCATCAATCT GGTTTCAATT TCCACTTCGC TCCGAGTTCT CGACTTTTCT 
GATACTGGTA TACATCTGGA CAAACTCTGG AATTCTTTAA AATTCGGTGG CCTTCAAATC 
GAAAAGCTAA TATTGAATGG TTGCTCATTG AGCAAAAAAT CAGAAGGAGT TCAAACCGCG 
AAGGAATATT TTTCGATGGC AGTCAATCTA TCGCATATTT CATTCAACAA CACGTCAATG 
CCATCTGAAT ATCTTAAAGC AGTTCTTCTT GGACTTGCTT CGAATCAACA ACTTCAACCA 
TTCCGTCTGG ATTTAGATGC GACGTGTGAG AAAGGATCCG CAAGTGTACT TGATGCATGC 
ATTGGAGGAA TTCGTTGTGA GACCCTCAGT CTTCGAGATA ATAATCTGGA TGGTGAACTG 
CAAGGAGTTT TGCAGAGTCT GATGATGGTT ACATGTCTAC GAAGACTTGA TATTGGAGGA 
TCTAATATGA ATCAATTGAA GAAAAACAAC AAACAAGTGC ATGTGATCAA TAAAGTTCTT 
CTGGATGTAG TAAAGCTATA CAGCGAGGAA GGGTGTCTCG AAGAGCTCAA TCTCTCGGAT 
TGCCGTCTCG GTGCATATCT AAGCGTACTT ATCAACACAT TGGCAGCAAC TACAACGCTC 
AAAAGTCTTG ATATAAGTGG AAATGAGATG GGAAACTTTG GAGCCAGAAT TCTTTCGAAA 
GCACTACAAG TTAATGTATC TCTTCGATCT GTGTCCATTG ACAATAATCA TATTGGTGCT 
GATGGTTTTG TCGATTTGGC AACGTCTATC AAGATGAACC ACACATTAAC TCATTTCCCT 
TATCCTGTTC ATGATGCATT TGACTGTATG CAGCGTGTAG AACGACCGAG GACAGTGGCA 
GCATTATCAC AGATTCAAAA CTGCTTGTAT CGGAATAGAA CAGCTCTCGG AGCCGACGAA 
GCCAATTGCA AGAAAATGTT CGCAGAGGGA GTGCTACAGA AAATGGAGAA AGCGAATGAT 
GAGGTTGTTG GAAGCATTGT TGACTCAATT TCAGCTTTTG GACAAGAACC ATTTCCACCA 
AAAATGATTG AAATCATTGA AGAATTCGTT GGAAAGTTTC AAATGGAAGG CGGTCGGATC 
ATTTCTGAGA GCATTTCAAG AACAATCGGA GGTGATTTAA GTGCAACATC GCGTCGTGCA 
GAGCAACTTG CAACAGCTAG ACTTGCTGAA GTTTGCACAG AACAAGTGAA ACATGTGTTT 
AGTGAATGGA AATGGAGGGA AATGTGCGAA CACGTGGAAT CTGAACTGTC CAAAATGTCA 
ATTGGAGGAT CTGGTGATGC ATGCAGCCTT GGAAGAACGT CATCTGTGGG GACAGGATCA 
CCTGCAATGT CATCTCCATT CACTCCGAAA CGTTCTCAGG CAGGACATCG TCCGAGAAGT 
ATAATTGGAG ATCTGACATC ATCGACAACA TCTTCCGAAA CAGTTGGAGA TTCTACGGAG 
CAAGGAGTCA ATTTAGATTT GCCACCAAAG CCTTCAGTCC TATCACATCT ACAAAAGGCA 
AGACCAAGGA AAAGAGGAGG AGCTTCATCT ATTCTAAATT CATCCCTTTC TGAAGACGTG 
ATGATGACAA GTAAATCAAG TGGAGGACCA GAGAATGGAG ATTTGGCTGA TCTAATTGAA 
GAAGTTGATG AAGTTGAACA AGAACCAAGA AAAGTTCCAA CTGGTGTTCG TGTTGCTATG 
ATACCAGATA GTTCCCTCAT TTCACAAGTG CATCTTCGTC CTTCAGCTGA ACGTACTCTT 
TCTGCTTCTT CTGACTTCTC ACTGTCTCCA CGTGAAGCTT CTTCTCCAAC AACAAGTAAC 
AGTAAGATCA TGACGTCATC AAGTGCTTCG GATTCTCCAC CACCTTTGCC TCAACGTAAT 
AGAGTTGGAC CTGGCGGTGC TCCACCCCCA TTACTTCCTC CTAAACCAGA ACCTCGAAGT 
CGTTTGATTG GAATGACGTC TCCAACTACA CCTCCACCTA CAGCAGATGA TGATGAAAAT 
GCAAATAGTC GTCGCTCTGT GGCTGATATG GCTAGAATTT TCCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crml-1 ( NM_001322686.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001309450.1 Caenorhabditis elegans Carm_PH domain-containing protein (crml-1), mRNA.

Price & Availability↑

CloneID	OCa335267
Clone ID Related Accession (Same CDS sequence)	NM_001322686.1 , NM_001322686.2
Accession Version	NM_001322686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Carm_PH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001322686.1.

ATGTCCATGT CGAGGAGCAC CATCCAGGAA TTGTGTGATT TCGTACAACA TAAACCATCA 
CAAATCCTAG GAAACAAATT CTTCGACTCG CGGATTGTGG TTCAGGTGGA TGTGGCACAC 
AAAGCAGATA AATATGAGCC ACGTATTTTT ATCATATCGA AATTTAGAAT ATTCTTTCTT 
ATGGGTAAAA CTACATCGTC ATTGAAAATC GAAAAATCAT TTCATGTGCT GGCGATAAAA 
GCGATTCATG TGATAAGTGA AGAAGAATTG GTGATTCATT TGGATGATGG TGTACATAAA 
AAGAAGATTA ATATTAAATG TTCAATTCAT CCAGCTGTTC ATTTGGCTAG ACAACTGTTA 
TCAGCTGTCA AACACTATTT TCCAGATTTC GGAGTTTATC TACAATCATC AATCGATGTG 
AATCCATCTG CTCTCTACAC AGAATTTCCA TCTCTTCCTA CAGCTTCTCC ACGCCCATGT 
CATTCTTTTC GGCGAACTTA CGCGGCTTTG TGTGATTTCT ATGATCAACC TTATCGAGAT 
GAAGTATCGT GGGATGTCGA GAAAATATAC ACAGCAAACA AATTACGAGA TTTGAAAATT 
GACGACTTCT CGCATCTCCT TCCAAAAGAT CTTCTTCCAA TCGTTGGCGT CCTTCAATAT 
TCAGCATATT TTACGGGACT TATCTGTGAT GCAGTCCGAG TATCCTCCGA AGTTATCGAT 
GTGGTGTTGA GTGTAATCCG AAAGTCACAA AATCTGAAAA AGCTTCAACT GAGGAGTTGC 
AGTCTCCCAA AAGATTTCAT TACTCTCCTT GCATCAGCTT TTCACAATAA CCAAAATGCC 
TGCCTCGAGT ATTTGGATCT TTCAAGGAAT CCGATTGATG ACAAAAAAGG ATTCACAATT 
TTGTCCTCCG TGCTTCCTCG GCTCAATCAG CTAAAATATG TCAACTTTTC TGAATGTCAA 
TTATCGGATA AATCTATTAA TTTGTTATGC ACTGGTCTAT ATAATGGAAT GACGTCATGC 
AAAAGTGGAG GAATGCAACT AACAGAGCTC ATTTTGTCAG CAAATCCTAT GAAGGATGAT 
ATATCTGGAA TCATCAATCT GGTTTCAATT TCCACTTCGC TCCGAGTTCT CGACTTTTCT 
GATACTGGTA TACATCTGGA CAAACTCTGG AATTCTTTAA AATTCGGTGG CCTTCAAATC 
GAAAAGCTAA TATTGAATGG TTGCTCATTG AGCAAAAAAT CAGAAGGAGT TCAAACCGCG 
AAGGAATATT TTTCGATGGC AGTCAATCTA TCGCATATTT CATTCAACAA CACGTCAATG 
CCATCTGAAT ATCTTAAAGC AGTTCTTCTT GGACTTGCTT CGAATCAACA ACTTCAACCA 
TTCCGTCTGG ATTTAGATGC GACGTGTGAG AAAGGATCCG CAAGTGTACT TGATGCATGC 
ATTGGAGGAA TTCGTTGTGA GACCCTCAGT CTTCGAGATA ATAATCTGGA TGGTGAACTG 
CAAGGAGTTT TGCAGAGTCT GATGATGGTT ACATGTCTAC GAAGACTTGA TATTGGAGGA 
TCTAATATGA ATCAATTGAA GAAAAACAAC AAACAAGTGC ATGTGATCAA TAAAGTTCTT 
CTGGATGTAG TAAAGCTATA CAGCGAGGAA GGGTGTCTCG AAGAGCTCAA TCTCTCGGAT 
TGCCGTCTCG GTGCATATCT AAGCGTACTT ATCAACACAT TGGCAGCAAC TACAACGCTC 
AAAAGTCTTG ATATAAGTGG AAATGAGATG GGAAACTTTG GAGCCAGAAT TCTTTCGAAA 
GCACTACAAG TTAATGTATC TCTTCGATCT GTGTCCATTG ACAATAATCA TATTGGTGCT 
GATGGTTTTG TCGATTTGGC AACGTCTATC AAGATGAACC ACACATTAAC TCATTTCCCT 
TATCCTGTTC ATGATGCATT TGACTGTATG CAGCGTGTAG AACGACCGAG GACAGTGGCA 
GCATTATCAC AGATTCAAAA CTGCTTGTAT CGGAATAGAA CAGCTCTCGG AGCCGACGAA 
GCCAATTGCA AGAAAATGTT CGCAGAGGGA GTGCTACAGA AAATGGAGAA AGCGAATGAT 
GAGGTTGTTG GAAGCATTGT TGACTCAATT TCAGCTTTTG GACAAGAACC ATTTCCACCA 
AAAATGATTG AAATCATTGA AGAATTCGTT GGAAAGTTTC AAATGGAAGG CGGTCGGATC 
ATTTCTGAGA GCATTTCAAG AACAATCGGA GGTGATTTAA GTGCAACATC GCGTCGTGCA 
GAGCAACTTG CAACAGCTAG ACTTGCTGAA GTTTGCACAG AACAAGTGAA ACATGTGTTT 
AGTGAATGGA AATGGAGGGA AATGTGCGAA CACGTGGAAT CTGAACTGTC CAAAATGTCA 
ATTGGAGGAT CTGGTGATGC ATGCAGCCTT GGAAGAACGT CATCTGTGGG GACAGGATCA 
CCTGCAATGT CATCTCCATT CACTCCGAAA CGTTCTCAGG CAGGACATCG TCCGAGAAGT 
ATAATTGGAG ATCTGACATC ATCGACAACA TCTTCCGAAA CAGTTGGAGA TTCTACGGAG 
CAAGGAGTCA ATTTAGATTT GCCACCAAAG CCTTCAGTCC TATCACATCT ACAAAAGGCA 
AGACCAAGGA AAAGAGGAGG AGCTTCATCT ATTCTAAATT CATCCCTTTC TGAAGACGTG 
ATGATGACAA GTAAATCAAG TGGAGGACCA GAGAATGGAG ATTTGGCTGA TCTAATTGAA 
GAAGTTGATG AAGTTGAACA AGAACCAAGA AAAGTTCCAA CTGGTGTTCG TGTTGCTATG 
ATACCAGATA GTTCCCTCAT TTCACAAGTG CATCTTCGTC CTTCAGCTGA ACGTACTCTT 
TCTGCTTCTT CTGACTTCTC ACTGTCTCCA CGTGAAGCTT CTTCTCCAAC AACAAGTAAC 
AGTAAGATCA TGACGTCATC AAGTGCTTCG GATTCTCCAC CACCTTTGCC TCAACGTAAT 
AGAGTTGGAC CTGGCGGTGC TCCACCCCCA TTACTTCCTC CTAAACCAGA ACCTCGAAGT 
CGTTTGATTG GAATGACGTC TCCAACTACA CCTCCACCTA CAGCAGATGA TGATGAAAAT 
GCAAATAGTC GTCGCTCTGT GGCTGATATG GCTAGAATTT TCCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309450.1
CDS	67..3237
Translation	MSMSRSTIQELCDFVQHKPSQILGNKFFDSRIVVQVDVAHKADKYEPRIFIISKFRIFFLMGKTTSSLKIEKSFHVLAIKAIHVISEEELVIHLDDGVHKKKINIKCSIHPAVHLARQLLSAVKHYFPDFGVYLQSSIDVNPSALYTEFPSLPTASPRPCHSFRRTYAALCDFYDQPYRDEVSWDVEKIYTANKLRDLKIDDFSHLLPKDLLPIVGVLQYSAYFTGLICDAVRVSSEVIDVVLSVIRKSQNLKKLQLRSCSLPKDFITLLASAFHNNQNACLEYLDLSRNPIDDKKGFTILSSVLPRLNQLKYVNFSECQLSDKSINLLCTGLYNGMTSCKSGGMQLTELILSANPMKDDISGIINLVSISTSLRVLDFSDTGIHLDKLWNSLKFGGLQIEKLILNGCSLSKKSEGVQTAKEYFSMAVNLSHISFNNTSMPSEYLKAVLLGLASNQQLQPFRLDLDATCEKGSASVLDACIGGIRCETLSLRDNNLDGELQGVLQSLMMVTCLRRLDIGGSNMNQLKKNNKQVHVINKVLLDVVKLYSEEGCLEELNLSDCRLGAYLSVLINTLAATTTLKSLDISGNEMGNFGARILSKALQVNVSLRSVSIDNNHIGADGFVDLATSIKMNHTLTHFPYPVHDAFDCMQRVERPRTVAALSQIQNCLYRNRTALGADEANCKKMFAEGVLQKMEKANDEVVGSIVDSISAFGQEPFPPKMIEIIEEFVGKFQMEGGRIISESISRTIGGDLSATSRRAEQLATARLAEVCTEQVKHVFSEWKWREMCEHVESELSKMSIGGSGDACSLGRTSSVGTGSPAMSSPFTPKRSQAGHRPRSIIGDLTSSTTSSETVGDSTEQGVNLDLPPKPSVLSHLQKARPRKRGGASSILNSSLSEDVMMTSKSSGGPENGDLADLIEEVDEVEQEPRKVPTGVRVAMIPDSSLISQVHLRPSAERTLSASSDFSLSPREASSPTTSNSKIMTSSSASDSPPPLPQRNRVGPGGAPPPLLPPKPEPRSRLIGMTSPTTPPPTADDDENANSRRSVADMARIFQK

Target ORF information:

RefSeq Version	NM_001322686.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Carm_PH domain-containing protein (crml-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322686.2

ORF Insert Sequence:

ATGTCCATGT CGAGGAGCAC CATCCAGGAA TTGTGTGATT TCGTACAACA TAAACCATCA 
CAAATCCTAG GAAACAAATT CTTCGACTCG CGGATTGTGG TTCAGGTGGA TGTGGCACAC 
AAAGCAGATA AATATGAGCC ACGTATTTTT ATCATATCGA AATTTAGAAT ATTCTTTCTT 
ATGGGTAAAA CTACATCGTC ATTGAAAATC GAAAAATCAT TTCATGTGCT GGCGATAAAA 
GCGATTCATG TGATAAGTGA AGAAGAATTG GTGATTCATT TGGATGATGG TGTACATAAA 
AAGAAGATTA ATATTAAATG TTCAATTCAT CCAGCTGTTC ATTTGGCTAG ACAACTGTTA 
TCAGCTGTCA AACACTATTT TCCAGATTTC GGAGTTTATC TACAATCATC AATCGATGTG 
AATCCATCTG CTCTCTACAC AGAATTTCCA TCTCTTCCTA CAGCTTCTCC ACGCCCATGT 
CATTCTTTTC GGCGAACTTA CGCGGCTTTG TGTGATTTCT ATGATCAACC TTATCGAGAT 
GAAGTATCGT GGGATGTCGA GAAAATATAC ACAGCAAACA AATTACGAGA TTTGAAAATT 
GACGACTTCT CGCATCTCCT TCCAAAAGAT CTTCTTCCAA TCGTTGGCGT CCTTCAATAT 
TCAGCATATT TTACGGGACT TATCTGTGAT GCAGTCCGAG TATCCTCCGA AGTTATCGAT 
GTGGTGTTGA GTGTAATCCG AAAGTCACAA AATCTGAAAA AGCTTCAACT GAGGAGTTGC 
AGTCTCCCAA AAGATTTCAT TACTCTCCTT GCATCAGCTT TTCACAATAA CCAAAATGCC 
TGCCTCGAGT ATTTGGATCT TTCAAGGAAT CCGATTGATG ACAAAAAAGG ATTCACAATT 
TTGTCCTCCG TGCTTCCTCG GCTCAATCAG CTAAAATATG TCAACTTTTC TGAATGTCAA 
TTATCGGATA AATCTATTAA TTTGTTATGC ACTGGTCTAT ATAATGGAAT GACGTCATGC 
AAAAGTGGAG GAATGCAACT AACAGAGCTC ATTTTGTCAG CAAATCCTAT GAAGGATGAT 
ATATCTGGAA TCATCAATCT GGTTTCAATT TCCACTTCGC TCCGAGTTCT CGACTTTTCT 
GATACTGGTA TACATCTGGA CAAACTCTGG AATTCTTTAA AATTCGGTGG CCTTCAAATC 
GAAAAGCTAA TATTGAATGG TTGCTCATTG AGCAAAAAAT CAGAAGGAGT TCAAACCGCG 
AAGGAATATT TTTCGATGGC AGTCAATCTA TCGCATATTT CATTCAACAA CACGTCAATG 
CCATCTGAAT ATCTTAAAGC AGTTCTTCTT GGACTTGCTT CGAATCAACA ACTTCAACCA 
TTCCGTCTGG ATTTAGATGC GACGTGTGAG AAAGGATCCG CAAGTGTACT TGATGCATGC 
ATTGGAGGAA TTCGTTGTGA GACCCTCAGT CTTCGAGATA ATAATCTGGA TGGTGAACTG 
CAAGGAGTTT TGCAGAGTCT GATGATGGTT ACATGTCTAC GAAGACTTGA TATTGGAGGA 
TCTAATATGA ATCAATTGAA GAAAAACAAC AAACAAGTGC ATGTGATCAA TAAAGTTCTT 
CTGGATGTAG TAAAGCTATA CAGCGAGGAA GGGTGTCTCG AAGAGCTCAA TCTCTCGGAT 
TGCCGTCTCG GTGCATATCT AAGCGTACTT ATCAACACAT TGGCAGCAAC TACAACGCTC 
AAAAGTCTTG ATATAAGTGG AAATGAGATG GGAAACTTTG GAGCCAGAAT TCTTTCGAAA 
GCACTACAAG TTAATGTATC TCTTCGATCT GTGTCCATTG ACAATAATCA TATTGGTGCT 
GATGGTTTTG TCGATTTGGC AACGTCTATC AAGATGAACC ACACATTAAC TCATTTCCCT 
TATCCTGTTC ATGATGCATT TGACTGTATG CAGCGTGTAG AACGACCGAG GACAGTGGCA 
GCATTATCAC AGATTCAAAA CTGCTTGTAT CGGAATAGAA CAGCTCTCGG AGCCGACGAA 
GCCAATTGCA AGAAAATGTT CGCAGAGGGA GTGCTACAGA AAATGGAGAA AGCGAATGAT 
GAGGTTGTTG GAAGCATTGT TGACTCAATT TCAGCTTTTG GACAAGAACC ATTTCCACCA 
AAAATGATTG AAATCATTGA AGAATTCGTT GGAAAGTTTC AAATGGAAGG CGGTCGGATC 
ATTTCTGAGA GCATTTCAAG AACAATCGGA GGTGATTTAA GTGCAACATC GCGTCGTGCA 
GAGCAACTTG CAACAGCTAG ACTTGCTGAA GTTTGCACAG AACAAGTGAA ACATGTGTTT 
AGTGAATGGA AATGGAGGGA AATGTGCGAA CACGTGGAAT CTGAACTGTC CAAAATGTCA 
ATTGGAGGAT CTGGTGATGC ATGCAGCCTT GGAAGAACGT CATCTGTGGG GACAGGATCA 
CCTGCAATGT CATCTCCATT CACTCCGAAA CGTTCTCAGG CAGGACATCG TCCGAGAAGT 
ATAATTGGAG ATCTGACATC ATCGACAACA TCTTCCGAAA CAGTTGGAGA TTCTACGGAG 
CAAGGAGTCA ATTTAGATTT GCCACCAAAG CCTTCAGTCC TATCACATCT ACAAAAGGCA 
AGACCAAGGA AAAGAGGAGG AGCTTCATCT ATTCTAAATT CATCCCTTTC TGAAGACGTG 
ATGATGACAA GTAAATCAAG TGGAGGACCA GAGAATGGAG ATTTGGCTGA TCTAATTGAA 
GAAGTTGATG AAGTTGAACA AGAACCAAGA AAAGTTCCAA CTGGTGTTCG TGTTGCTATG 
ATACCAGATA GTTCCCTCAT TTCACAAGTG CATCTTCGTC CTTCAGCTGA ACGTACTCTT 
TCTGCTTCTT CTGACTTCTC ACTGTCTCCA CGTGAAGCTT CTTCTCCAAC AACAAGTAAC 
AGTAAGATCA TGACGTCATC AAGTGCTTCG GATTCTCCAC CACCTTTGCC TCAACGTAAT 
AGAGTTGGAC CTGGCGGTGC TCCACCCCCA TTACTTCCTC CTAAACCAGA ACCTCGAAGT 
CGTTTGATTG GAATGACGTC TCCAACTACA CCTCCACCTA CAGCAGATGA TGATGAAAAT 
GCAAATAGTC GTCGCTCTGT GGCTGATATG GCTAGAATTT TCCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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crml-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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