pdi-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pdi-3
Entrez Gene ID	172433
Full Name	Protein disulfide-isomerase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059594.6	NP_491995.1	Protein disulfide-isomerase
NM_059594.7	NP_491995.1	Protein disulfide-isomerase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDIA3	NP_001233381.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007393	XP_564835.2
Caenorhabditis elegans (roundworm)	pdi-3	NP_491995.1
Xenopus tropicalis (tropical clawed frog)	pdia3	NP_989329.1
Homo sapiens (human)	PDIA3	NP_005304.3
	PDIA3	XP_001109061.1
Canis lupus familiaris (dog)	PDIA3	XP_535453.3
Mus musculus (house mouse)	Pdia3	NP_031978.2
Drosophila melanogaster (fruit fly)	ERp60	NP_725084.2
Bos taurus (cattle)	PDIA3	NP_776758.2
Danio rerio (zebrafish)	pdia3	NP_001186666.1
Oryza sativa (rice)	Os02g0554900	NP_001047119.1
Rattus norvegicus (Norway rat)	Pdia3	NP_059015.1
Gallus gallus (chicken)	PDIA3	NP_989441.1
Oryza sativa (rice)	Os04g0436300	NP_001052852.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pdi-1, pdi-2, and pdi-3 have comparable kinetic properties in catalyzing thiol-disulfide exchange reactions in two simple peptide-based assays. However, the three enzymes exhibited clear differences in their reactivity towards protein substrates.
Title: Protein disulfide isomerases from C. elegans are equally efficient at thiol-disulfide exchange in simple peptide-based systems but show differences in reactivity towards protein substrates.

Thioredoxin motif of PDI-3 provides Cys and His catalytic residues for transglutaminase activity.
Title: Thioredoxin motif of Caenorhabditis elegans PDI-3 provides Cys and His catalytic residues for transglutaminase activity.

determination of disulfide isomerase and transglutaminase-like cross linking activity and involvement in maintaining body morphology.
Title: The Caenorhabditis elegans ERp60 homolog protein disulfide isomerase-3 has disulfide isomerase and transglutaminase-like cross-linking activity and is involved in the maintenance of body morphology.

The following pdi-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pdi-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335866	NM_059594.7 Latest version!	Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCa335866	NM_059594.6	Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pdi-3 ( NM_059594.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491995.1 Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), partial mRNA.

Price & Availability↑

CloneID	OCa335866
Clone ID Related Accession (Same CDS sequence)	NM_059594.6 , NM_059594.7
Accession Version	NM_059594.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein disulfide-isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059594.5. COMPLETENESS: incomplete on both ends.

ATGATTTGGG TCCAGGCAGC TTTGGTCGCC TCCTTCCTGG CGTTCGCCAG TGCTGGAGGA 
GCCGTTCTCG AGTATACCGA CGGAAACTTC GATGATCTCA TCCAAACCCA CGACATTGCT 
CTTGTCAAGT TCTACGCTCC CTGGTGCGGA CACTGCAAGA AAATTGCCCC AGAGTATGAG 
AGAGCTGCCC CAAAACTCGC ATCTAACGAT CCACCAGTCG CTCTCGTCAA GGTCGATTGT 
ACCACCGAGA AGACTGTTTG TGACAAATTC GGAGTTAAGG GATTCCCAAC TCTTAAGATC 
TTCCGCAATG GAGTTCCAGC CCAAGACTAC GATGGACCAC GTGATGCCGA TGGTATCGTT 
AAGTTCATGC GCGGACAGTC GGGACCTTCT TCCAAGGAAC TTAAGACCGT TGCCGAATTC 
GAGAAGTTCA CTGGTGGAGA TGAAAATGTT GTCATCGGAT TCTTCGAGAG CGAAAGCAAG 
CTTAAGGATT CTTATCTCAA GGTTGCTGAT ACCGAACGTG ATCGTTTCTC CTTCGCTCAC 
ACTTCCAACA AAGACATCAT CAAGAAGGCT GGATACTCTG ATGACGTCGT TGTCTTCGTT 
CCAAAAAAGC TCCATAACAA GTTTGACACT AACGAGTTCA AATATGATGG TAACTACGAC 
ACCGATAAGA TTAAGAACTT CTTGGTCCAT GAAACTGTTG GATTTGCCGG AATTCGTACT 
CAAGGAAACT TGTTCCAATT CGAGCAAAAG CCAATCGTCA TTGTTTACTA CAACGTTGAT 
TACGTCAAGG ATCCTAAGGG ATCCAACTAC TGGCGCAACC GTGTCTTGAA AGTTGCTCAA 
AACTATAAGA GAAAAGTTCA ATTCGCCGTT TCCAACAAAG AAGAATTCTC TTCTGAAATT 
GAAACCAATG GACTAGGAGA GCGCAAGGAC TCGGATAAGC CAATCGTTGC CATTCTCACT 
AATGAAGGAA AGTATCCAAT GGACCAAGAA TTCAGCGTTG ACAATCTCCA GCAATTCGTT 
GATGAAGTTC TCGCCGGAAA TGCCGAACCA TACATGAAGT CTGAGCCAAT CCCAGATGAA 
CAAGGAGACG TTAAGGTTGC CGTTGGAAAG AACTTCAAGG AACTTATCAT GGATGCTGAT 
AAGGATGTGC TCATTGAATT TTATGCTCCA TGGTGCGGAC ATTGCAAATC ATTGGCTCCA 
AAATACGAGG AACTTGCTGA GAAGCTCAAC AAGGAAGATG TTATCATTGC TAAGATGGAT 
GCCACCGCCA ATGATGTTCC ACCAATGTTC GAAGTTAGAG GATTCCCAAC TCTCTTCTGG 
CTTCCAAAGA ACGCTAAGTC GAACCCGATC CCATACAATG GAGGACGCGA AGTTAAGGAT 
TTCGTCAGCT TCATCTCAAA GCACTCTACT GATGGGCTCA AGGGTTTCAG CCGCGACGGA 
AAGAAGAAGA AGAAGACTGA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491995.1
CDS	1..1467
Translation	MIWVQAALVASFLAFASAGGAVLEYTDGNFDDLIQTHDIALVKFYAPWCGHCKKIAPEYERAAPKLASNDPPVALVKVDCTTEKTVCDKFGVKGFPTLKIFRNGVPAQDYDGPRDADGIVKFMRGQSGPSSKELKTVAEFEKFTGGDENVVIGFFESESKLKDSYLKVADTERDRFSFAHTSNKDIIKKAGYSDDVVVFVPKKLHNKFDTNEFKYDGNYDTDKIKNFLVHETVGFAGIRTQGNLFQFEQKPIVIVYYNVDYVKDPKGSNYWRNRVLKVAQNYKRKVQFAVSNKEEFSSEIETNGLGERKDSDKPIVAILTNEGKYPMDQEFSVDNLQQFVDEVLAGNAEPYMKSEPIPDEQGDVKVAVGKNFKELIMDADKDVLIEFYAPWCGHCKSLAPKYEELAEKLNKEDVIIAKMDATANDVPPMFEVRGFPTLFWLPKNAKSNPIPYNGGREVKDFVSFISKHSTDGLKGFSRDGKKKKKTEL

Target ORF information:

RefSeq Version	NM_059594.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059594.6

ORF Insert Sequence:

ATGATTTGGG TCCAGGCAGC TTTGGTCGCC TCCTTCCTGG CGTTCGCCAG TGCTGGAGGA 
GCCGTTCTCG AGTATACCGA CGGAAACTTC GATGATCTCA TCCAAACCCA CGACATTGCT 
CTTGTCAAGT TCTACGCTCC CTGGTGCGGA CACTGCAAGA AAATTGCCCC AGAGTATGAG 
AGAGCTGCCC CAAAACTCGC ATCTAACGAT CCACCAGTCG CTCTCGTCAA GGTCGATTGT 
ACCACCGAGA AGACTGTTTG TGACAAATTC GGAGTTAAGG GATTCCCAAC TCTTAAGATC 
TTCCGCAATG GAGTTCCAGC CCAAGACTAC GATGGACCAC GTGATGCCGA TGGTATCGTT 
AAGTTCATGC GCGGACAGTC GGGACCTTCT TCCAAGGAAC TTAAGACCGT TGCCGAATTC 
GAGAAGTTCA CTGGTGGAGA TGAAAATGTT GTCATCGGAT TCTTCGAGAG CGAAAGCAAG 
CTTAAGGATT CTTATCTCAA GGTTGCTGAT ACCGAACGTG ATCGTTTCTC CTTCGCTCAC 
ACTTCCAACA AAGACATCAT CAAGAAGGCT GGATACTCTG ATGACGTCGT TGTCTTCGTT 
CCAAAAAAGC TCCATAACAA GTTTGACACT AACGAGTTCA AATATGATGG TAACTACGAC 
ACCGATAAGA TTAAGAACTT CTTGGTCCAT GAAACTGTTG GATTTGCCGG AATTCGTACT 
CAAGGAAACT TGTTCCAATT CGAGCAAAAG CCAATCGTCA TTGTTTACTA CAACGTTGAT 
TACGTCAAGG ATCCTAAGGG ATCCAACTAC TGGCGCAACC GTGTCTTGAA AGTTGCTCAA 
AACTATAAGA GAAAAGTTCA ATTCGCCGTT TCCAACAAAG AAGAATTCTC TTCTGAAATT 
GAAACCAATG GACTAGGAGA GCGCAAGGAC TCGGATAAGC CAATCGTTGC CATTCTCACT 
AATGAAGGAA AGTATCCAAT GGACCAAGAA TTCAGCGTTG ACAATCTCCA GCAATTCGTT 
GATGAAGTTC TCGCCGGAAA TGCCGAACCA TACATGAAGT CTGAGCCAAT CCCAGATGAA 
CAAGGAGACG TTAAGGTTGC CGTTGGAAAG AACTTCAAGG AACTTATCAT GGATGCTGAT 
AAGGATGTGC TCATTGAATT TTATGCTCCA TGGTGCGGAC ATTGCAAATC ATTGGCTCCA 
AAATACGAGG AACTTGCTGA GAAGCTCAAC AAGGAAGATG TTATCATTGC TAAGATGGAT 
GCCACCGCCA ATGATGTTCC ACCAATGTTC GAAGTTAGAG GATTCCCAAC TCTCTTCTGG 
CTTCCAAAGA ACGCTAAGTC GAACCCGATC CCATACAATG GAGGACGCGA AGTTAAGGAT 
TTCGTCAGCT TCATCTCAAA GCACTCTACT GATGGGCTCA AGGGTTTCAG CCGCGACGGA 
AAGAAGAAGA AGAAGACTGA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdi-3 ( NM_059594.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491995.1 Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), mRNA.

Price & Availability↑

CloneID	OCa335866
Clone ID Related Accession (Same CDS sequence)	NM_059594.6 , NM_059594.7
Accession Version	NM_059594.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein disulfide-isomerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059594.6.

ATGATTTGGG TCCAGGCAGC TTTGGTCGCC TCCTTCCTGG CGTTCGCCAG TGCTGGAGGA 
GCCGTTCTCG AGTATACCGA CGGAAACTTC GATGATCTCA TCCAAACCCA CGACATTGCT 
CTTGTCAAGT TCTACGCTCC CTGGTGCGGA CACTGCAAGA AAATTGCCCC AGAGTATGAG 
AGAGCTGCCC CAAAACTCGC ATCTAACGAT CCACCAGTCG CTCTCGTCAA GGTCGATTGT 
ACCACCGAGA AGACTGTTTG TGACAAATTC GGAGTTAAGG GATTCCCAAC TCTTAAGATC 
TTCCGCAATG GAGTTCCAGC CCAAGACTAC GATGGACCAC GTGATGCCGA TGGTATCGTT 
AAGTTCATGC GCGGACAGTC GGGACCTTCT TCCAAGGAAC TTAAGACCGT TGCCGAATTC 
GAGAAGTTCA CTGGTGGAGA TGAAAATGTT GTCATCGGAT TCTTCGAGAG CGAAAGCAAG 
CTTAAGGATT CTTATCTCAA GGTTGCTGAT ACCGAACGTG ATCGTTTCTC CTTCGCTCAC 
ACTTCCAACA AAGACATCAT CAAGAAGGCT GGATACTCTG ATGACGTCGT TGTCTTCGTT 
CCAAAAAAGC TCCATAACAA GTTTGACACT AACGAGTTCA AATATGATGG TAACTACGAC 
ACCGATAAGA TTAAGAACTT CTTGGTCCAT GAAACTGTTG GATTTGCCGG AATTCGTACT 
CAAGGAAACT TGTTCCAATT CGAGCAAAAG CCAATCGTCA TTGTTTACTA CAACGTTGAT 
TACGTCAAGG ATCCTAAGGG ATCCAACTAC TGGCGCAACC GTGTCTTGAA AGTTGCTCAA 
AACTATAAGA GAAAAGTTCA ATTCGCCGTT TCCAACAAAG AAGAATTCTC TTCTGAAATT 
GAAACCAATG GACTAGGAGA GCGCAAGGAC TCGGATAAGC CAATCGTTGC CATTCTCACT 
AATGAAGGAA AGTATCCAAT GGACCAAGAA TTCAGCGTTG ACAATCTCCA GCAATTCGTT 
GATGAAGTTC TCGCCGGAAA TGCCGAACCA TACATGAAGT CTGAGCCAAT CCCAGATGAA 
CAAGGAGACG TTAAGGTTGC CGTTGGAAAG AACTTCAAGG AACTTATCAT GGATGCTGAT 
AAGGATGTGC TCATTGAATT TTATGCTCCA TGGTGCGGAC ATTGCAAATC ATTGGCTCCA 
AAATACGAGG AACTTGCTGA GAAGCTCAAC AAGGAAGATG TTATCATTGC TAAGATGGAT 
GCCACCGCCA ATGATGTTCC ACCAATGTTC GAAGTTAGAG GATTCCCAAC TCTCTTCTGG 
CTTCCAAAGA ACGCTAAGTC GAACCCGATC CCATACAATG GAGGACGCGA AGTTAAGGAT 
TTCGTCAGCT TCATCTCAAA GCACTCTACT GATGGGCTCA AGGGTTTCAG CCGCGACGGA 
AAGAAGAAGA AGAAGACTGA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491995.1
CDS	11..1477
Translation	MIWVQAALVASFLAFASAGGAVLEYTDGNFDDLIQTHDIALVKFYAPWCGHCKKIAPEYERAAPKLASNDPPVALVKVDCTTEKTVCDKFGVKGFPTLKIFRNGVPAQDYDGPRDADGIVKFMRGQSGPSSKELKTVAEFEKFTGGDENVVIGFFESESKLKDSYLKVADTERDRFSFAHTSNKDIIKKAGYSDDVVVFVPKKLHNKFDTNEFKYDGNYDTDKIKNFLVHETVGFAGIRTQGNLFQFEQKPIVIVYYNVDYVKDPKGSNYWRNRVLKVAQNYKRKVQFAVSNKEEFSSEIETNGLGERKDSDKPIVAILTNEGKYPMDQEFSVDNLQQFVDEVLAGNAEPYMKSEPIPDEQGDVKVAVGKNFKELIMDADKDVLIEFYAPWCGHCKSLAPKYEELAEKLNKEDVIIAKMDATANDVPPMFEVRGFPTLFWLPKNAKSNPIPYNGGREVKDFVSFISKHSTDGLKGFSRDGKKKKKTEL

Target ORF information:

RefSeq Version	NM_059594.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein disulfide-isomerase (pdi-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059594.7

ORF Insert Sequence:

ATGATTTGGG TCCAGGCAGC TTTGGTCGCC TCCTTCCTGG CGTTCGCCAG TGCTGGAGGA 
GCCGTTCTCG AGTATACCGA CGGAAACTTC GATGATCTCA TCCAAACCCA CGACATTGCT 
CTTGTCAAGT TCTACGCTCC CTGGTGCGGA CACTGCAAGA AAATTGCCCC AGAGTATGAG 
AGAGCTGCCC CAAAACTCGC ATCTAACGAT CCACCAGTCG CTCTCGTCAA GGTCGATTGT 
ACCACCGAGA AGACTGTTTG TGACAAATTC GGAGTTAAGG GATTCCCAAC TCTTAAGATC 
TTCCGCAATG GAGTTCCAGC CCAAGACTAC GATGGACCAC GTGATGCCGA TGGTATCGTT 
AAGTTCATGC GCGGACAGTC GGGACCTTCT TCCAAGGAAC TTAAGACCGT TGCCGAATTC 
GAGAAGTTCA CTGGTGGAGA TGAAAATGTT GTCATCGGAT TCTTCGAGAG CGAAAGCAAG 
CTTAAGGATT CTTATCTCAA GGTTGCTGAT ACCGAACGTG ATCGTTTCTC CTTCGCTCAC 
ACTTCCAACA AAGACATCAT CAAGAAGGCT GGATACTCTG ATGACGTCGT TGTCTTCGTT 
CCAAAAAAGC TCCATAACAA GTTTGACACT AACGAGTTCA AATATGATGG TAACTACGAC 
ACCGATAAGA TTAAGAACTT CTTGGTCCAT GAAACTGTTG GATTTGCCGG AATTCGTACT 
CAAGGAAACT TGTTCCAATT CGAGCAAAAG CCAATCGTCA TTGTTTACTA CAACGTTGAT 
TACGTCAAGG ATCCTAAGGG ATCCAACTAC TGGCGCAACC GTGTCTTGAA AGTTGCTCAA 
AACTATAAGA GAAAAGTTCA ATTCGCCGTT TCCAACAAAG AAGAATTCTC TTCTGAAATT 
GAAACCAATG GACTAGGAGA GCGCAAGGAC TCGGATAAGC CAATCGTTGC CATTCTCACT 
AATGAAGGAA AGTATCCAAT GGACCAAGAA TTCAGCGTTG ACAATCTCCA GCAATTCGTT 
GATGAAGTTC TCGCCGGAAA TGCCGAACCA TACATGAAGT CTGAGCCAAT CCCAGATGAA 
CAAGGAGACG TTAAGGTTGC CGTTGGAAAG AACTTCAAGG AACTTATCAT GGATGCTGAT 
AAGGATGTGC TCATTGAATT TTATGCTCCA TGGTGCGGAC ATTGCAAATC ATTGGCTCCA 
AAATACGAGG AACTTGCTGA GAAGCTCAAC AAGGAAGATG TTATCATTGC TAAGATGGAT 
GCCACCGCCA ATGATGTTCC ACCAATGTTC GAAGTTAGAG GATTCCCAAC TCTCTTCTGG 
CTTCCAAAGA ACGCTAAGTC GAACCCGATC CCATACAATG GAGGACGCGA AGTTAAGGAT 
TTCGTCAGCT TCATCTCAAA GCACTCTACT GATGGGCTCA AGGGTTTCAG CCGCGACGGA 
AAGAAGAAGA AGAAGACTGA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pdi-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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