lin-35 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	lin-35
Entrez Gene ID	172249
Full Name	Retinoblastoma-like protein homolog lin-35
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059285.3	NP_491686.1	Retinoblastoma-like protein homolog lin-35
NM_059285.4	NP_491686.1	Retinoblastoma-like protein homolog lin-35

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RBL1	NP_001179531.1
Mus musculus (house mouse)	Rbl1	NP_035379.2
Danio rerio (zebrafish)	rbl1	NP_001124082.1
Oryza sativa (rice)	Os11g0533500	NP_001068020.1
Xenopus tropicalis (tropical clawed frog)	rbl1	NP_001135587.1
Homo sapiens (human)	RBL1	NP_002886.2
	RBL1	XP_002798195.1
Rattus norvegicus (Norway rat)	Rbl1	NP_001177995.1
Caenorhabditis elegans (roundworm)	lin-35	NP_491686.1
Arabidopsis thaliana (thale cress)	RBR1	NP_566417.3
Pan troglodytes (chimpanzee)	RBL1	XP_001139919.1
Canis lupus familiaris (dog)	RBL1	XP_534412.2
Gallus gallus (chicken)	RBL1	XP_417312.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports that LIN-35 indeed mediates the association of E2F-DP and MuvB, a function that stabilizes (Dp/Retinoblastoma(Rb)-like/E2F/MuvB) or DREAM transcriptional repressor complex subunit occupancy at target genes. In the absence of LIN-35, the occupancy of E2F-DP and MuvB at most DREAM target genes decreases dramatically and many of those genes become upregulated.
Title: Loss of the Caenorhabditis elegans pocket protein LIN-35 reveals MuvB's innate function as the repressor of DREAM target genes.

Simultaneous mutation of lin-35 and fzr-1, an orthologue to Cdh1, completely eliminates the essential requirement of CDK4/6-cyclin D (CDK-4/CYD-1) in C. elegans.
Title: Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells.

CED-9/Bcl-2 downregulation via LIN-35/Rb triggers germ cell apoptosis in C. elegans in response to starvation.
Title: LIN-35/Rb causes starvation-induced germ cell apoptosis via CED-9/Bcl2 downregulation in Caenorhabditis elegans.

Tumor suppressor Rb maintains the "starvation-induced" transcriptome and represses the "refeeding-induced" transcriptome, including the repression of many pathogen-, toxin-, and oxidative-stress-inducible and metabolic genes.
Title: The tumor suppressor Rb critically regulates starvation-induced stress response in C. elegans.

The function of LIN-35/Rb is to prevent germline gene expression in the soma
Title: Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes.

The following lin-35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lin-35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa350603	NM_059285.4 Latest version!	Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa350603	NM_059285.3	Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lin-35 ( NM_059285.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491686.1 Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), partial mRNA.

Price & Availability↑

CloneID	OCa350603
Clone ID Related Accession (Same CDS sequence)	NM_059285.3 , NM_059285.4
Accession Version	NM_059285.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Retinoblastoma-like protein homolog lin-35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059285.2. COMPLETENESS: incomplete on both ends.

ATGCCGAAAC GAGCAGCCGA TGAGCCTGGA ACATCAACAA CTGACCCATT TCACGAGCAA 
AGCCCATTCG ATGCCGTGTT AGCCGGCACG GAGACAACGG ATACAATATG TGAAGAGCCA 
CCAGCAAAAC GAATCGACTT AGATATAAAG CAAGAATTCA ATGGTGGAGT GCAAAGTGGA 
GGGCTGATTA AAAATGAATC CGAATTGACT CAAATGACAA TCAAACAAGA AACAGAAGGA 
AACATAAATG AAGCTAGACG AGAAGAAGAA GACGAAGAAC AAGATGAAGA TTCCAGAACA 
TCAATGCCAC CTGCATTGGG AGAAGATGAT GATTATGAGG AGGATGATGC TGATAGTTTT 
ATTGATAAAA CTAATACACC GCCACCATCA CAATCATTTC TGGAAGGATG TCGAGCAGCT 
AATTTACCAA ATGACATTGT TACTGGTGCA TGGGAAACGT ACAACCACGC GGTTCAACGG 
GTTTCTCTTG AGGGTTCGGA ATCGGCGTGG CAACTATCAG CAATTTACTA TTATCTTCTA 
TCAAAAGGAA TAAAACGTCG TGGAAAAACA ATCCGTATTC TCATTCAACC GTTTCCTGTT 
TCGATACTTA CAATTGCCAA CTCATTTGAC ATATCCGTTG CTGAAATGCT TGACAAAACT 
GCTCGATTTG TGGAAATTAT ACATTCCAGA AAAATTCGTC GTTATCAAGA ATATATTCGA 
CGAATTCAAG AAGGACTCGC AGTTTCTTGT GTGATATTCA AAAAGTTTTG CCGAATTTTC 
TGCAAAATAT TCGAGGAGAT CAAAGTTGGA TCCGAAAATT GTCCATCTTC TCATGAACTT 
TTTACGGTTC TTTGGACATC TTTTCTGGTG ATGAAAAGTC GAATGACAGT GGACGATTTG 
ATTTCAAATT ATCAACTTCT TTTTTCAATA CTTGATCAAG TATATACCGA AATGTGTTCA 
ATGAAAGAGG GAATAGTTCA TCATTTGAAT CAAAAATTTG TTGAAGATCT TCTTGAAAAT 
GATTGTACGA TTATTCGAGC TCTTTGCACA CAATTTGGTG GAAGTGTTCT TGATGCACGG 
CACTTTTCTG ATCATACTTT TAAGAAAATG GAGAAGACTG GAATTCCGTC CACTTGGAAT 
TTTCAAGAGT TTCGAGATTT GATCATGAAC GTTCCAAAAA CGGCATATGA GAATTATCTA 
TTGCAACGTG GAAGTATTGA TGAGCGGATT TTCATTCCAA GCGTTGAGGA CTTTTCAAAA 
ATTTTCCAAT CCCCGGACAC ATACTCAGTA GCAGATATTC TCAAAGTGTC TTACTCTGGA 
AGACGTTTCC GTGATGCAGA ATTTCTTACA AAAATCTCAA ATAATCATTG TCTGGAAAAG 
TTGGCATTAG GTGGAAAAGT AGCATCAGAA AAGTTGGTAA CACAGTCAAA AGAACAGCCG 
AGAGTTCCGT GTGTTGAGTA TAATCTCGAA TTGGGAAATT ATCCAGACGA TTTGGAATCG 
AACAATCAAA GTCTTTATAA TAGATTGACA AAAATTATTG GAAGCTGGAA ATTGGAGAAT 
TCGAAACTCG AAGAAGTGTG TGGCACAATG TCCGACAGTC CAATGGCAAC AATTCTTCTG 
AAAAGTGATG AAATGACAAA TAAATTCGAG CGAACTTTAT CTGCAGAACT CGGAGAGACG 
ATCAATGAGA ATATTCCTAA ATATCACTAT AATGTTCGAA AAGAATTGGA ATTAGTTTTT 
CTCATTTTCA TGGAGAAAAT AATTGTTGCA GAATTGAAAA AGAAAGTACG AGAGGAGGAC 
TTGCTGAATG TGATTCGTCG GGAAGAATTT CTTGATTCAG TTTTCTGTTT CTGTGTTGAA 
CTGATCCTTG TTTCCAATGG ATATGATCGT CCATTTCCAT GGAGTGCTGA ACTGTGTGGA 
GTACATCCAT TTATGTTTCA TAAAGTAATT GATTTGATGA TAACACATGA GAAACAGCTA 
AGTCGTCAAA TGGTTCAACA TTTTAGTCGA ATTGAAGAAA CTGTAATTGA GTATTTTTCG 
TGGAAGTCTG ATAGTCCATT ATGGCCAATG GTTGTCAGGT GTCCATTTGC ACATTTTCAA 
GAATTCGGAG AGGATTGGGC TGATAAATTA AACTCGTACT CACCAATAAA ATTCACTCCA 
ATCAAGAAGC CTGATGATCT ACGAGACGAA CTTGGAAGAC CTATAGTTCC TCAAAATCAA 
ACTTCAAGAA CTCTAAGAAT TTTTTTGAAA AGAACTTATT TCACCGCCGC TCGTCGACTT 
CAAGATCTCA CTGATCGTGT TTCAATGGGA GCTCGTGCAA AATCACAATG CTGGTCACTT 
TTCGATTATC TTCTTCGCAA TGACACTTTG ATTTTTATGG ATAGACATCT TGATCAAATT 
CTTCTTTGTT GCGTGTTTGT CATTATGAAG ATAAATGAGT CATCAATGCT TTTCACGGAA 
ATAATGGCTC AATATCGACG ACAATCAGCC AATTCTTTGC TGGTCTACCG AAGTGTTACA 
GTATTCCAAG AACAACTGAA TCCCGAAAAT CCACAGGCAG TAAACACGAA GGAGACAATT 
TTGGAACGTC TTGAAGGTCC ACAAAAAGAA AAAACGACAG TTGATATAAT CAAATATTAT 
AATATCGAGT TTCGGGATCG TATCAAGTAT ATTATCGGTC AAATTGATAG TGCTTCAGAT 
GAAGATTTGA TGGAAATGCC GGTTGCAACA GAATCTGGAT TGATGCCTGT TCGAGTTTAT 
TTAACACATA AATTATCGAT TCAAACGCTT CCAAAAACGA AACACGGAGA GTCGAAACAA 
GAAAGAGCTA TTGCGAACCT TGAAAAATCT GGAATTACGA TCGCTATGGA ACGGTCTGGA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491686.1
CDS	1..2886
Translation	MPKRAADEPGTSTTDPFHEQSPFDAVLAGTETTDTICEEPPAKRIDLDIKQEFNGGVQSGGLIKNESELTQMTIKQETEGNINEARREEEDEEQDEDSRTSMPPALGEDDDYEEDDADSFIDKTNTPPPSQSFLEGCRAANLPNDIVTGAWETYNHAVQRVSLEGSESAWQLSAIYYYLLSKGIKRRGKTIRILIQPFPVSILTIANSFDISVAEMLDKTARFVEIIHSRKIRRYQEYIRRIQEGLAVSCVIFKKFCRIFCKIFEEIKVGSENCPSSHELFTVLWTSFLVMKSRMTVDDLISNYQLLFSILDQVYTEMCSMKEGIVHHLNQKFVEDLLENDCTIIRALCTQFGGSVLDARHFSDHTFKKMEKTGIPSTWNFQEFRDLIMNVPKTAYENYLLQRGSIDERIFIPSVEDFSKIFQSPDTYSVADILKVSYSGRRFRDAEFLTKISNNHCLEKLALGGKVASEKLVTQSKEQPRVPCVEYNLELGNYPDDLESNNQSLYNRLTKIIGSWKLENSKLEEVCGTMSDSPMATILLKSDEMTNKFERTLSAELGETINENIPKYHYNVRKELELVFLIFMEKIIVAELKKKVREEDLLNVIRREEFLDSVFCFCVELILVSNGYDRPFPWSAELCGVHPFMFHKVIDLMITHEKQLSRQMVQHFSRIEETVIEYFSWKSDSPLWPMVVRCPFAHFQEFGEDWADKLNSYSPIKFTPIKKPDDLRDELGRPIVPQNQTSRTLRIFLKRTYFTAARRLQDLTDRVSMGARAKSQCWSLFDYLLRNDTLIFMDRHLDQILLCCVFVIMKINESSMLFTEIMAQYRRQSANSLLVYRSVTVFQEQLNPENPQAVNTKETILERLEGPQKEKTTVDIIKYYNIEFRDRIKYIIGQIDSASDEDLMEMPVATESGLMPVRVYLTHKLSIQTLPKTKHGESKQERAIANLEKSGITIAMERSGD

Target ORF information:

RefSeq Version	NM_059285.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059285.3

ORF Insert Sequence:

ATGCCGAAAC GAGCAGCCGA TGAGCCTGGA ACATCAACAA CTGACCCATT TCACGAGCAA 
AGCCCATTCG ATGCCGTGTT AGCCGGCACG GAGACAACGG ATACAATATG TGAAGAGCCA 
CCAGCAAAAC GAATCGACTT AGATATAAAG CAAGAATTCA ATGGTGGAGT GCAAAGTGGA 
GGGCTGATTA AAAATGAATC CGAATTGACT CAAATGACAA TCAAACAAGA AACAGAAGGA 
AACATAAATG AAGCTAGACG AGAAGAAGAA GACGAAGAAC AAGATGAAGA TTCCAGAACA 
TCAATGCCAC CTGCATTGGG AGAAGATGAT GATTATGAGG AGGATGATGC TGATAGTTTT 
ATTGATAAAA CTAATACACC GCCACCATCA CAATCATTTC TGGAAGGATG TCGAGCAGCT 
AATTTACCAA ATGACATTGT TACTGGTGCA TGGGAAACGT ACAACCACGC GGTTCAACGG 
GTTTCTCTTG AGGGTTCGGA ATCGGCGTGG CAACTATCAG CAATTTACTA TTATCTTCTA 
TCAAAAGGAA TAAAACGTCG TGGAAAAACA ATCCGTATTC TCATTCAACC GTTTCCTGTT 
TCGATACTTA CAATTGCCAA CTCATTTGAC ATATCCGTTG CTGAAATGCT TGACAAAACT 
GCTCGATTTG TGGAAATTAT ACATTCCAGA AAAATTCGTC GTTATCAAGA ATATATTCGA 
CGAATTCAAG AAGGACTCGC AGTTTCTTGT GTGATATTCA AAAAGTTTTG CCGAATTTTC 
TGCAAAATAT TCGAGGAGAT CAAAGTTGGA TCCGAAAATT GTCCATCTTC TCATGAACTT 
TTTACGGTTC TTTGGACATC TTTTCTGGTG ATGAAAAGTC GAATGACAGT GGACGATTTG 
ATTTCAAATT ATCAACTTCT TTTTTCAATA CTTGATCAAG TATATACCGA AATGTGTTCA 
ATGAAAGAGG GAATAGTTCA TCATTTGAAT CAAAAATTTG TTGAAGATCT TCTTGAAAAT 
GATTGTACGA TTATTCGAGC TCTTTGCACA CAATTTGGTG GAAGTGTTCT TGATGCACGG 
CACTTTTCTG ATCATACTTT TAAGAAAATG GAGAAGACTG GAATTCCGTC CACTTGGAAT 
TTTCAAGAGT TTCGAGATTT GATCATGAAC GTTCCAAAAA CGGCATATGA GAATTATCTA 
TTGCAACGTG GAAGTATTGA TGAGCGGATT TTCATTCCAA GCGTTGAGGA CTTTTCAAAA 
ATTTTCCAAT CCCCGGACAC ATACTCAGTA GCAGATATTC TCAAAGTGTC TTACTCTGGA 
AGACGTTTCC GTGATGCAGA ATTTCTTACA AAAATCTCAA ATAATCATTG TCTGGAAAAG 
TTGGCATTAG GTGGAAAAGT AGCATCAGAA AAGTTGGTAA CACAGTCAAA AGAACAGCCG 
AGAGTTCCGT GTGTTGAGTA TAATCTCGAA TTGGGAAATT ATCCAGACGA TTTGGAATCG 
AACAATCAAA GTCTTTATAA TAGATTGACA AAAATTATTG GAAGCTGGAA ATTGGAGAAT 
TCGAAACTCG AAGAAGTGTG TGGCACAATG TCCGACAGTC CAATGGCAAC AATTCTTCTG 
AAAAGTGATG AAATGACAAA TAAATTCGAG CGAACTTTAT CTGCAGAACT CGGAGAGACG 
ATCAATGAGA ATATTCCTAA ATATCACTAT AATGTTCGAA AAGAATTGGA ATTAGTTTTT 
CTCATTTTCA TGGAGAAAAT AATTGTTGCA GAATTGAAAA AGAAAGTACG AGAGGAGGAC 
TTGCTGAATG TGATTCGTCG GGAAGAATTT CTTGATTCAG TTTTCTGTTT CTGTGTTGAA 
CTGATCCTTG TTTCCAATGG ATATGATCGT CCATTTCCAT GGAGTGCTGA ACTGTGTGGA 
GTACATCCAT TTATGTTTCA TAAAGTAATT GATTTGATGA TAACACATGA GAAACAGCTA 
AGTCGTCAAA TGGTTCAACA TTTTAGTCGA ATTGAAGAAA CTGTAATTGA GTATTTTTCG 
TGGAAGTCTG ATAGTCCATT ATGGCCAATG GTTGTCAGGT GTCCATTTGC ACATTTTCAA 
GAATTCGGAG AGGATTGGGC TGATAAATTA AACTCGTACT CACCAATAAA ATTCACTCCA 
ATCAAGAAGC CTGATGATCT ACGAGACGAA CTTGGAAGAC CTATAGTTCC TCAAAATCAA 
ACTTCAAGAA CTCTAAGAAT TTTTTTGAAA AGAACTTATT TCACCGCCGC TCGTCGACTT 
CAAGATCTCA CTGATCGTGT TTCAATGGGA GCTCGTGCAA AATCACAATG CTGGTCACTT 
TTCGATTATC TTCTTCGCAA TGACACTTTG ATTTTTATGG ATAGACATCT TGATCAAATT 
CTTCTTTGTT GCGTGTTTGT CATTATGAAG ATAAATGAGT CATCAATGCT TTTCACGGAA 
ATAATGGCTC AATATCGACG ACAATCAGCC AATTCTTTGC TGGTCTACCG AAGTGTTACA 
GTATTCCAAG AACAACTGAA TCCCGAAAAT CCACAGGCAG TAAACACGAA GGAGACAATT 
TTGGAACGTC TTGAAGGTCC ACAAAAAGAA AAAACGACAG TTGATATAAT CAAATATTAT 
AATATCGAGT TTCGGGATCG TATCAAGTAT ATTATCGGTC AAATTGATAG TGCTTCAGAT 
GAAGATTTGA TGGAAATGCC GGTTGCAACA GAATCTGGAT TGATGCCTGT TCGAGTTTAT 
TTAACACATA AATTATCGAT TCAAACGCTT CCAAAAACGA AACACGGAGA GTCGAAACAA 
GAAAGAGCTA TTGCGAACCT TGAAAAATCT GGAATTACGA TCGCTATGGA ACGGTCTGGA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lin-35 ( NM_059285.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491686.1 Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), mRNA.

Price & Availability↑

CloneID	OCa350603
Clone ID Related Accession (Same CDS sequence)	NM_059285.3 , NM_059285.4
Accession Version	NM_059285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Retinoblastoma-like protein homolog lin-35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059285.3.

ATGCCGAAAC GAGCAGCCGA TGAGCCTGGA ACATCAACAA CTGACCCATT TCACGAGCAA 
AGCCCATTCG ATGCCGTGTT AGCCGGCACG GAGACAACGG ATACAATATG TGAAGAGCCA 
CCAGCAAAAC GAATCGACTT AGATATAAAG CAAGAATTCA ATGGTGGAGT GCAAAGTGGA 
GGGCTGATTA AAAATGAATC CGAATTGACT CAAATGACAA TCAAACAAGA AACAGAAGGA 
AACATAAATG AAGCTAGACG AGAAGAAGAA GACGAAGAAC AAGATGAAGA TTCCAGAACA 
TCAATGCCAC CTGCATTGGG AGAAGATGAT GATTATGAGG AGGATGATGC TGATAGTTTT 
ATTGATAAAA CTAATACACC GCCACCATCA CAATCATTTC TGGAAGGATG TCGAGCAGCT 
AATTTACCAA ATGACATTGT TACTGGTGCA TGGGAAACGT ACAACCACGC GGTTCAACGG 
GTTTCTCTTG AGGGTTCGGA ATCGGCGTGG CAACTATCAG CAATTTACTA TTATCTTCTA 
TCAAAAGGAA TAAAACGTCG TGGAAAAACA ATCCGTATTC TCATTCAACC GTTTCCTGTT 
TCGATACTTA CAATTGCCAA CTCATTTGAC ATATCCGTTG CTGAAATGCT TGACAAAACT 
GCTCGATTTG TGGAAATTAT ACATTCCAGA AAAATTCGTC GTTATCAAGA ATATATTCGA 
CGAATTCAAG AAGGACTCGC AGTTTCTTGT GTGATATTCA AAAAGTTTTG CCGAATTTTC 
TGCAAAATAT TCGAGGAGAT CAAAGTTGGA TCCGAAAATT GTCCATCTTC TCATGAACTT 
TTTACGGTTC TTTGGACATC TTTTCTGGTG ATGAAAAGTC GAATGACAGT GGACGATTTG 
ATTTCAAATT ATCAACTTCT TTTTTCAATA CTTGATCAAG TATATACCGA AATGTGTTCA 
ATGAAAGAGG GAATAGTTCA TCATTTGAAT CAAAAATTTG TTGAAGATCT TCTTGAAAAT 
GATTGTACGA TTATTCGAGC TCTTTGCACA CAATTTGGTG GAAGTGTTCT TGATGCACGG 
CACTTTTCTG ATCATACTTT TAAGAAAATG GAGAAGACTG GAATTCCGTC CACTTGGAAT 
TTTCAAGAGT TTCGAGATTT GATCATGAAC GTTCCAAAAA CGGCATATGA GAATTATCTA 
TTGCAACGTG GAAGTATTGA TGAGCGGATT TTCATTCCAA GCGTTGAGGA CTTTTCAAAA 
ATTTTCCAAT CCCCGGACAC ATACTCAGTA GCAGATATTC TCAAAGTGTC TTACTCTGGA 
AGACGTTTCC GTGATGCAGA ATTTCTTACA AAAATCTCAA ATAATCATTG TCTGGAAAAG 
TTGGCATTAG GTGGAAAAGT AGCATCAGAA AAGTTGGTAA CACAGTCAAA AGAACAGCCG 
AGAGTTCCGT GTGTTGAGTA TAATCTCGAA TTGGGAAATT ATCCAGACGA TTTGGAATCG 
AACAATCAAA GTCTTTATAA TAGATTGACA AAAATTATTG GAAGCTGGAA ATTGGAGAAT 
TCGAAACTCG AAGAAGTGTG TGGCACAATG TCCGACAGTC CAATGGCAAC AATTCTTCTG 
AAAAGTGATG AAATGACAAA TAAATTCGAG CGAACTTTAT CTGCAGAACT CGGAGAGACG 
ATCAATGAGA ATATTCCTAA ATATCACTAT AATGTTCGAA AAGAATTGGA ATTAGTTTTT 
CTCATTTTCA TGGAGAAAAT AATTGTTGCA GAATTGAAAA AGAAAGTACG AGAGGAGGAC 
TTGCTGAATG TGATTCGTCG GGAAGAATTT CTTGATTCAG TTTTCTGTTT CTGTGTTGAA 
CTGATCCTTG TTTCCAATGG ATATGATCGT CCATTTCCAT GGAGTGCTGA ACTGTGTGGA 
GTACATCCAT TTATGTTTCA TAAAGTAATT GATTTGATGA TAACACATGA GAAACAGCTA 
AGTCGTCAAA TGGTTCAACA TTTTAGTCGA ATTGAAGAAA CTGTAATTGA GTATTTTTCG 
TGGAAGTCTG ATAGTCCATT ATGGCCAATG GTTGTCAGGT GTCCATTTGC ACATTTTCAA 
GAATTCGGAG AGGATTGGGC TGATAAATTA AACTCGTACT CACCAATAAA ATTCACTCCA 
ATCAAGAAGC CTGATGATCT ACGAGACGAA CTTGGAAGAC CTATAGTTCC TCAAAATCAA 
ACTTCAAGAA CTCTAAGAAT TTTTTTGAAA AGAACTTATT TCACCGCCGC TCGTCGACTT 
CAAGATCTCA CTGATCGTGT TTCAATGGGA GCTCGTGCAA AATCACAATG CTGGTCACTT 
TTCGATTATC TTCTTCGCAA TGACACTTTG ATTTTTATGG ATAGACATCT TGATCAAATT 
CTTCTTTGTT GCGTGTTTGT CATTATGAAG ATAAATGAGT CATCAATGCT TTTCACGGAA 
ATAATGGCTC AATATCGACG ACAATCAGCC AATTCTTTGC TGGTCTACCG AAGTGTTACA 
GTATTCCAAG AACAACTGAA TCCCGAAAAT CCACAGGCAG TAAACACGAA GGAGACAATT 
TTGGAACGTC TTGAAGGTCC ACAAAAAGAA AAAACGACAG TTGATATAAT CAAATATTAT 
AATATCGAGT TTCGGGATCG TATCAAGTAT ATTATCGGTC AAATTGATAG TGCTTCAGAT 
GAAGATTTGA TGGAAATGCC GGTTGCAACA GAATCTGGAT TGATGCCTGT TCGAGTTTAT 
TTAACACATA AATTATCGAT TCAAACGCTT CCAAAAACGA AACACGGAGA GTCGAAACAA 
GAAAGAGCTA TTGCGAACCT TGAAAAATCT GGAATTACGA TCGCTATGGA ACGGTCTGGA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491686.1
CDS	22..2907
Translation	MPKRAADEPGTSTTDPFHEQSPFDAVLAGTETTDTICEEPPAKRIDLDIKQEFNGGVQSGGLIKNESELTQMTIKQETEGNINEARREEEDEEQDEDSRTSMPPALGEDDDYEEDDADSFIDKTNTPPPSQSFLEGCRAANLPNDIVTGAWETYNHAVQRVSLEGSESAWQLSAIYYYLLSKGIKRRGKTIRILIQPFPVSILTIANSFDISVAEMLDKTARFVEIIHSRKIRRYQEYIRRIQEGLAVSCVIFKKFCRIFCKIFEEIKVGSENCPSSHELFTVLWTSFLVMKSRMTVDDLISNYQLLFSILDQVYTEMCSMKEGIVHHLNQKFVEDLLENDCTIIRALCTQFGGSVLDARHFSDHTFKKMEKTGIPSTWNFQEFRDLIMNVPKTAYENYLLQRGSIDERIFIPSVEDFSKIFQSPDTYSVADILKVSYSGRRFRDAEFLTKISNNHCLEKLALGGKVASEKLVTQSKEQPRVPCVEYNLELGNYPDDLESNNQSLYNRLTKIIGSWKLENSKLEEVCGTMSDSPMATILLKSDEMTNKFERTLSAELGETINENIPKYHYNVRKELELVFLIFMEKIIVAELKKKVREEDLLNVIRREEFLDSVFCFCVELILVSNGYDRPFPWSAELCGVHPFMFHKVIDLMITHEKQLSRQMVQHFSRIEETVIEYFSWKSDSPLWPMVVRCPFAHFQEFGEDWADKLNSYSPIKFTPIKKPDDLRDELGRPIVPQNQTSRTLRIFLKRTYFTAARRLQDLTDRVSMGARAKSQCWSLFDYLLRNDTLIFMDRHLDQILLCCVFVIMKINESSMLFTEIMAQYRRQSANSLLVYRSVTVFQEQLNPENPQAVNTKETILERLEGPQKEKTTVDIIKYYNIEFRDRIKYIIGQIDSASDEDLMEMPVATESGLMPVRVYLTHKLSIQTLPKTKHGESKQERAIANLEKSGITIAMERSGD

Target ORF information:

RefSeq Version	NM_059285.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Retinoblastoma-like protein homolog lin-35 (lin-35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059285.4

ORF Insert Sequence:

ATGCCGAAAC GAGCAGCCGA TGAGCCTGGA ACATCAACAA CTGACCCATT TCACGAGCAA 
AGCCCATTCG ATGCCGTGTT AGCCGGCACG GAGACAACGG ATACAATATG TGAAGAGCCA 
CCAGCAAAAC GAATCGACTT AGATATAAAG CAAGAATTCA ATGGTGGAGT GCAAAGTGGA 
GGGCTGATTA AAAATGAATC CGAATTGACT CAAATGACAA TCAAACAAGA AACAGAAGGA 
AACATAAATG AAGCTAGACG AGAAGAAGAA GACGAAGAAC AAGATGAAGA TTCCAGAACA 
TCAATGCCAC CTGCATTGGG AGAAGATGAT GATTATGAGG AGGATGATGC TGATAGTTTT 
ATTGATAAAA CTAATACACC GCCACCATCA CAATCATTTC TGGAAGGATG TCGAGCAGCT 
AATTTACCAA ATGACATTGT TACTGGTGCA TGGGAAACGT ACAACCACGC GGTTCAACGG 
GTTTCTCTTG AGGGTTCGGA ATCGGCGTGG CAACTATCAG CAATTTACTA TTATCTTCTA 
TCAAAAGGAA TAAAACGTCG TGGAAAAACA ATCCGTATTC TCATTCAACC GTTTCCTGTT 
TCGATACTTA CAATTGCCAA CTCATTTGAC ATATCCGTTG CTGAAATGCT TGACAAAACT 
GCTCGATTTG TGGAAATTAT ACATTCCAGA AAAATTCGTC GTTATCAAGA ATATATTCGA 
CGAATTCAAG AAGGACTCGC AGTTTCTTGT GTGATATTCA AAAAGTTTTG CCGAATTTTC 
TGCAAAATAT TCGAGGAGAT CAAAGTTGGA TCCGAAAATT GTCCATCTTC TCATGAACTT 
TTTACGGTTC TTTGGACATC TTTTCTGGTG ATGAAAAGTC GAATGACAGT GGACGATTTG 
ATTTCAAATT ATCAACTTCT TTTTTCAATA CTTGATCAAG TATATACCGA AATGTGTTCA 
ATGAAAGAGG GAATAGTTCA TCATTTGAAT CAAAAATTTG TTGAAGATCT TCTTGAAAAT 
GATTGTACGA TTATTCGAGC TCTTTGCACA CAATTTGGTG GAAGTGTTCT TGATGCACGG 
CACTTTTCTG ATCATACTTT TAAGAAAATG GAGAAGACTG GAATTCCGTC CACTTGGAAT 
TTTCAAGAGT TTCGAGATTT GATCATGAAC GTTCCAAAAA CGGCATATGA GAATTATCTA 
TTGCAACGTG GAAGTATTGA TGAGCGGATT TTCATTCCAA GCGTTGAGGA CTTTTCAAAA 
ATTTTCCAAT CCCCGGACAC ATACTCAGTA GCAGATATTC TCAAAGTGTC TTACTCTGGA 
AGACGTTTCC GTGATGCAGA ATTTCTTACA AAAATCTCAA ATAATCATTG TCTGGAAAAG 
TTGGCATTAG GTGGAAAAGT AGCATCAGAA AAGTTGGTAA CACAGTCAAA AGAACAGCCG 
AGAGTTCCGT GTGTTGAGTA TAATCTCGAA TTGGGAAATT ATCCAGACGA TTTGGAATCG 
AACAATCAAA GTCTTTATAA TAGATTGACA AAAATTATTG GAAGCTGGAA ATTGGAGAAT 
TCGAAACTCG AAGAAGTGTG TGGCACAATG TCCGACAGTC CAATGGCAAC AATTCTTCTG 
AAAAGTGATG AAATGACAAA TAAATTCGAG CGAACTTTAT CTGCAGAACT CGGAGAGACG 
ATCAATGAGA ATATTCCTAA ATATCACTAT AATGTTCGAA AAGAATTGGA ATTAGTTTTT 
CTCATTTTCA TGGAGAAAAT AATTGTTGCA GAATTGAAAA AGAAAGTACG AGAGGAGGAC 
TTGCTGAATG TGATTCGTCG GGAAGAATTT CTTGATTCAG TTTTCTGTTT CTGTGTTGAA 
CTGATCCTTG TTTCCAATGG ATATGATCGT CCATTTCCAT GGAGTGCTGA ACTGTGTGGA 
GTACATCCAT TTATGTTTCA TAAAGTAATT GATTTGATGA TAACACATGA GAAACAGCTA 
AGTCGTCAAA TGGTTCAACA TTTTAGTCGA ATTGAAGAAA CTGTAATTGA GTATTTTTCG 
TGGAAGTCTG ATAGTCCATT ATGGCCAATG GTTGTCAGGT GTCCATTTGC ACATTTTCAA 
GAATTCGGAG AGGATTGGGC TGATAAATTA AACTCGTACT CACCAATAAA ATTCACTCCA 
ATCAAGAAGC CTGATGATCT ACGAGACGAA CTTGGAAGAC CTATAGTTCC TCAAAATCAA 
ACTTCAAGAA CTCTAAGAAT TTTTTTGAAA AGAACTTATT TCACCGCCGC TCGTCGACTT 
CAAGATCTCA CTGATCGTGT TTCAATGGGA GCTCGTGCAA AATCACAATG CTGGTCACTT 
TTCGATTATC TTCTTCGCAA TGACACTTTG ATTTTTATGG ATAGACATCT TGATCAAATT 
CTTCTTTGTT GCGTGTTTGT CATTATGAAG ATAAATGAGT CATCAATGCT TTTCACGGAA 
ATAATGGCTC AATATCGACG ACAATCAGCC AATTCTTTGC TGGTCTACCG AAGTGTTACA 
GTATTCCAAG AACAACTGAA TCCCGAAAAT CCACAGGCAG TAAACACGAA GGAGACAATT 
TTGGAACGTC TTGAAGGTCC ACAAAAAGAA AAAACGACAG TTGATATAAT CAAATATTAT 
AATATCGAGT TTCGGGATCG TATCAAGTAT ATTATCGGTC AAATTGATAG TGCTTCAGAT 
GAAGATTTGA TGGAAATGCC GGTTGCAACA GAATCTGGAT TGATGCCTGT TCGAGTTTAT 
TTAACACATA AATTATCGAT TCAAACGCTT CCAAAAACGA AACACGGAGA GTCGAAACAA 
GAAAGAGCTA TTGCGAACCT TGAAAAATCT GGAATTACGA TCGCTATGGA ACGGTCTGGA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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lin-35 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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