let-363 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-363
Entrez Gene ID	172167
Full Name	Target of rapamycin homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059151.6	NP_491552.2	Target of rapamycin homolog
NM_059148.5	NP_491549.2	Target of rapamycin homolog
NM_059148.4	NP_491549.2	Target of rapamycin homolog
NM_059151.5	NP_491552.2	Target of rapamycin homolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dafachronic acid signaling through let-363 is essential for diet restriction-mediated longevity.
Title: Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans.

Mutations result in dauer-like larval arrest, implying that CeTOR (let-363)regulates dauer diapause.
Title: The TOR pathway interacts with the insulin signaling pathway to regulate C. elegans larval development, metabolism and life span.

C. elegans target of rapamycin (TOR) and the DAF-2/insulin-signaling pathways cross-talk with pep-2
Title: Deletion of the intestinal peptide transporter affects insulin and TOR signaling in Caenorhabditis elegans.

influence on lifespan
Title: Genetics: influence of TOR kinase on lifespan in C. elegans.

The following let-363 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-363 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336351	NM_059148.5 Latest version!	Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa336352	NM_059151.6 Latest version!	Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCa336351	NM_059148.4	Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa336352	NM_059151.5	Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-363 ( NM_059148.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491549.2 Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.

Price & Availability↑

CloneID	OCa336351
Clone ID Related Accession (Same CDS sequence)	NM_059148.5 , NM_059148.4
Accession Version	NM_059148.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Target of rapamycin homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059148.4.

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG TAATTATAGA TTACCCTGTT 
CCACCACCAC TTGGTTCACT CGTCGGTCTT CCACCAATGC CAGCTTATTT GTCTTCGAAT 
TCATCACTTC CACCACAACA TCATCATGTC TCACCATTGA GCAACGATTC TCCATCGAAT 
TCAGCTGAAA ATAAGCTTTA TTTGAAACAC GCGGCTCACG CTGTGCGATG CTTCGCAAAG 
GCTCTGATGT GCTCACCGGG TTCTCGACTT GAAGATACTC TCCGATTGAT GCAATTGTGG 
TTTGATCATG GAGATGATAA AGATCAAGAT GTTTATTTTG CTCTTACGGA GACAATCTTT 
GATCTTCCTG TGACAACATG GTTAGAAGCT ATTCCTCAAT TGATGGCTCG TTTGGATTGT 
CCAGATGATC AGAAAAGTGT TCAACTTGTT CTTCGAGTTC TTTGTGAAAT TGCCAGGCAT 
AGACCACAAG CAGTAATCTA TGCACTAACT GTTGCATCGA GAAGTAAGGA TGTACATAGA 
AGTAAGAATG CTGGAACAGT TCTCGAAAAA ATGATGGAAT ATCATTCAAA ATTGGTTCGA 
GAGGCAAGTC TTGTGACAGA AGAGCTTGTT CGTTGCGCAA TTCTGTGGCA TGAACAGTGG 
CATGATGCAC TTGATGATGC GAGCCGTGTG TATTTCCACG ATAACAATGT GCAGGCGATG 
TTTGATGCTT TGAGAAATAT GAATGATTTG ATGCAAAAAG GAGCTCCAAC AACCATGAAA 
GAGCACAGTT TCCAACAGAC GTATAGTTCC GATTTGAAAG AAGCAGGACG ATATGTACAA 
GCATTTGAGA GTAGTGGAAA TGTGAAAGAT CTTAATCAGG CGTGGGAGAT TTATTGTTCG 
GTTTTCAAAA AGCTGCGTGA TCAACTTGCC ACTTTGAACT CGTTGGATCT TGTCTACGTA 
TCACCTAATT TGGTCAGCGC AAAAGATTTG GAACTCGTTG TTCCTGGTAC ATACGATCCA 
TCAGCTCCAA TAGTTTCCAT TCAAAGTTTC AGCTCAAAAA TGAATGTAAT CACTAGCAAG 
CAACGTCCTC GTAAAATGGT GATCCGTGGA TCAAATGGTT TAGACTATCA ATTCTTGCTA 
AAAGGACATG AAGATCCGAG ACAAGATGAA CGTGTGATGC AACTTTTTGG ACTAGTCAAC 
ACACTTCTTG CCAACAATTC TGAGACTTGT CGCCGCAATT TGACTATTCA AAGATACTCG 
ATTGTAGCTT TGAGCAAAGA TTCTGGATTG ATTGGTTGGG TTCCAAATTG TGATACACTT 
CATACACTTG TCAAGGAATA TCGAGAAAAG AAAGCAAAGA TTCCATTGTC GATTGAGCAC 
AAAACACTTC AGAAATTATC ACTTGAAACT GAACATTTGA CTTTAATGCA GAAGCTTCAA 
TTATTTGAAA GTGCTCTAAG TGTAACACAA GGAGAAGATC TCCGACATGT ATTATGGTTG 
AAGAGTCCGA GTTCTGAAGT TTGGTTTGAT CGACGAACCA ATTATACAAG ATCAGTTGCA 
TGTATGTCAA TGGTTGGATA TATTCTTGGT CTCGGTGACA GACATCCATC CAACTTGATG 
CTCGATCGAT TGACCGGAAA AGTTGTACAC ATTGATTTCG GTGACTGTTT CGAAGTTGCT 
ATGCTCCGTG AAAAATTCCC TGAACGAGTT CCATTCCGAC TTACCAGAAT GCTCATCAAC 
GCTATGGAAG TAACTGGATT GGACGGAGTA TACAATTACA CTGCCGAACG TGTCTTGAAA 
ATGTTGCGAA CAAATCAAGA ATCATTGTTA GCAGTTTTAG AAGCATTTGT CTATGATCCA 
GTAATTAATT GGCGTCTTGT GGAAGGAATG AAAAAGGATC CGAAAACTAG AAAAGATACA 
GGTGGACGTC AGAATATGGC TGGTGCTGTA TTACCATCAT CAAGTACAAC TGATTCAATT 
ATGGAGACGA TTAAGAGGAA ACTCGATGGG ACCGAATTCG TTCACACTGA CGGATCAACT 
CCACCTGAAC CACTTCAAGT AACTGAGCAA CTTGCAATGC TCACAGAGCA AGCAACATCT 
CCATTGAATT TGTGTCAAAG TTATATTGGA TGGTGTCCGT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491549.2
CDS	39..8126
Translation	MLQQHGISFQMNADRQNKAATTSNRIVSAPATNEFDERHRKQGIDAARALASQYRSRITENRDANQRQAARELSRYVRSELKDEPNTFSDAFLNAIDGRTDIASQSAIYNCMKSNSNIDQKRAGIYLIVCLAETHSGNVIRYANYLLKMLNNGNGLDEDTVKMASKALAFLIATCKSYAAELVDRCLDHCHEWLGQNVPHSQQPKNQQEIDQIRRLAASHLSRELALATPTAFFLRVNLFFKYIFNAVRDKNPAVRIAGIDALHVVLTIVSQREAKNKTEWFKKCFDEALEGQPNPSQKDDLDRWHAVALILNELLRISDQRFELIRCESSQFIKQKFLKEDEEEGVEWLVLTKQQTIVESVTARKLVLERFPKILDCVRQIIPLANKTSSTKQQSSIYLNTVLMQLLPRICAFPQCDRTFQTISFDTSFTILQRNAVAAPAIGMMMLSNPDVHATHIEKTISFISAAIKKTTNSDVLDNYFTFLFLFVDAYHEQVTTQIKAIIPQLMDITLSRSLANVLKMIMMRIPKLRLNVQDGVMASVYQTLTGSLIPPKSEPIGRPASPKAILQKAETDPKELQRIVLAVDVLGEFYFSRGALQRIMQYVADYYLTADNVEIRLAAVSSCCEMVVPFVGVYKKVTSDKRNSLLQTIYGVLRAVCSVIVNDQDVRVRMQVISCFGQMPRPFLAHLAQPEMLEVQFMALHDEKLEMQQACVTLLGRLAELNPALVLPRLRLMLLETLSQMQQSGQARLEQHSAKMIAQLAKQSPKFMRPYVGSLMIAMIPKLRNDQKYAEVTAQVLNAVSEIAVIGGAEIVKNLKPLFEKLTHMINDSSSLHKREAALRAIGGICRSTAYVVDPYRDYPSLLDDLLRILKTVMSNTMRREAIKTLGILGAIDPYTHKVFTGSVQSSTAISTALSLPISETDSKDPRQDIIHWFNYEKCTLEEFYPAITIANLMLMMQDEDSQSYAEIAQAIVTIFRSLGDMAPLYTEQVIPRLIEVCRRATESSNRANLREFFLQQLANFVAIIRKHAAPYMPAIFTIIADAWKEDISVKMVVIEVLTDMGTAIGNDFSKYTGELIPYLLTVLQTDKTKERVLTVKVMESIQKLTHCIVQHLHLVLPPLLIILDDFSLKLSIRNTALSTVLHMTQQVDVSAYAPRMMQSWHHNISTAEMRDKLLLLLIEIIKQLGKFFDIFKRGVDQKLRDYNLDKSVHYEQYRKLAQRAQMSRDVLTSSVFAGSNGNIQYSSTQAGMRGQANNVYANNDLHERLMNGSIDSGASRQDNRDDYYRYGVEEKISEEVPKVAPTTARPTSELVTVQITKQRLNKDALMPQWKNENLTSKDEWLQWLMKIRIGFLTYGSSPSLRAASSLGDQHPHLARDLFPAAFMSVWTELDSDVQNDLTSCLLRAISTGIPELIQTILNLAEFMDHSEKGPLPISHDVLGRWAEQTKAFAKACRYKEMSVLKKSGSMQTTFTRKVKLEPNDCQSLITYANKLNVQEEAAGVVRYAERNEMNFQMRGRWYEKLNEWEKALGAYELEEKKKSSCPNLQVYDEKDHLMTPEEAATAEEARMHEMRCLEALGRWDELNSKSVVWADQRGNRNDSVRDEINKKQLDHKMAVIAARGAWAVDNWERMADYVSVISENTQDGAMLRAVVAVHNDENTKAMGLIEKVREMIDSELTAMANESYERAYIPMVSVQQMAELEEAIEYKTRPERRPRIALLWSRRLQGCRRNVEQWQRLIMLRGLVLSPQEMHPLRVKFSSMCRKQGKNSMSRAVLRELLSLPANSDLVRAKAPFDKPLLVLALAKQLYQDDHKDEAIRALEDLANHWNKRINPIPKATGRELIPPSTKEPARICAKVLLKLGEWTELKSKTSNNMQVGELSFVRQQVSPQYRTKESRTPETIAFENTINYYQQATQYDPGWHKVWHKLASTHFYAVCRERPHPTTVISPPQQPQQPKKMHIPPVTRATSPPPPAQKSPQPAPFHSITEPLSVIIDYPVPPPLGSLVGLPPMPAYLSSNSSLPPQHHHVSPLSNDSPSNSAENKLYLKHAAHAVRCFAKALMCSPGSRLEDTLRLMQLWFDHGDDKDQDVYFALTETIFDLPVTTWLEAIPQLMARLDCPDDQKSVQLVLRVLCEIARHRPQAVIYALTVASRSKDVHRSKNAGTVLEKMMEYHSKLVREASLVTEELVRCAILWHEQWHDALDDASRVYFHDNNVQAMFDALRNMNDLMQKGAPTTMKEHSFQQTYSSDLKEAGRYVQAFESSGNVKDLNQAWEIYCSVFKKLRDQLATLNSLDLVYVSPNLVSAKDLELVVPGTYDPSAPIVSIQSFSSKMNVITSKQRPRKMVIRGSNGLDYQFLLKGHEDPRQDERVMQLFGLVNTLLANNSETCRRNLTIQRYSIVALSKDSGLIGWVPNCDTLHTLVKEYREKKAKIPLSIEHKTLQKLSLETEHLTLMQKLQLFESALSVTQGEDLRHVLWLKSPSSEVWFDRRTNYTRSVACMSMVGYILGLGDRHPSNLMLDRLTGKVVHIDFGDCFEVAMLREKFPERVPFRLTRMLINAMEVTGLDGVYNYTAERVLKMLRTNQESLLAVLEAFVYDPVINWRLVEGMKKDPKTRKDTGGRQNMAGAVLPSSSTTDSIMETIKRKLDGTEFVHTDGSTPPEPLQVTEQLAMLTEQATSPLNLCQSYIGWCPFW

Target ORF information:

RefSeq Version	NM_059148.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059148.5

ORF Insert Sequence:

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG TAATTATAGA TTACCCTGTT 
CCACCACCAC TTGGTTCACT CGTCGGTCTT CCACCAATGC CAGCTTATTT GTCTTCGAAT 
TCATCACTTC CACCACAACA TCATCATGTC TCACCATTGA GCAACGATTC TCCATCGAAT 
TCAGCTGAAA ATAAGCTTTA TTTGAAACAC GCGGCTCACG CTGTGCGATG CTTCGCAAAG 
GCTCTGATGT GCTCACCGGG TTCTCGACTT GAAGATACTC TCCGATTGAT GCAATTGTGG 
TTTGATCATG GAGATGATAA AGATCAAGAT GTTTATTTTG CTCTTACGGA GACAATCTTT 
GATCTTCCTG TGACAACATG GTTAGAAGCT ATTCCTCAAT TGATGGCTCG TTTGGATTGT 
CCAGATGATC AGAAAAGTGT TCAACTTGTT CTTCGAGTTC TTTGTGAAAT TGCCAGGCAT 
AGACCACAAG CAGTAATCTA TGCACTAACT GTTGCATCGA GAAGTAAGGA TGTACATAGA 
AGTAAGAATG CTGGAACAGT TCTCGAAAAA ATGATGGAAT ATCATTCAAA ATTGGTTCGA 
GAGGCAAGTC TTGTGACAGA AGAGCTTGTT CGTTGCGCAA TTCTGTGGCA TGAACAGTGG 
CATGATGCAC TTGATGATGC GAGCCGTGTG TATTTCCACG ATAACAATGT GCAGGCGATG 
TTTGATGCTT TGAGAAATAT GAATGATTTG ATGCAAAAAG GAGCTCCAAC AACCATGAAA 
GAGCACAGTT TCCAACAGAC GTATAGTTCC GATTTGAAAG AAGCAGGACG ATATGTACAA 
GCATTTGAGA GTAGTGGAAA TGTGAAAGAT CTTAATCAGG CGTGGGAGAT TTATTGTTCG 
GTTTTCAAAA AGCTGCGTGA TCAACTTGCC ACTTTGAACT CGTTGGATCT TGTCTACGTA 
TCACCTAATT TGGTCAGCGC AAAAGATTTG GAACTCGTTG TTCCTGGTAC ATACGATCCA 
TCAGCTCCAA TAGTTTCCAT TCAAAGTTTC AGCTCAAAAA TGAATGTAAT CACTAGCAAG 
CAACGTCCTC GTAAAATGGT GATCCGTGGA TCAAATGGTT TAGACTATCA ATTCTTGCTA 
AAAGGACATG AAGATCCGAG ACAAGATGAA CGTGTGATGC AACTTTTTGG ACTAGTCAAC 
ACACTTCTTG CCAACAATTC TGAGACTTGT CGCCGCAATT TGACTATTCA AAGATACTCG 
ATTGTAGCTT TGAGCAAAGA TTCTGGATTG ATTGGTTGGG TTCCAAATTG TGATACACTT 
CATACACTTG TCAAGGAATA TCGAGAAAAG AAAGCAAAGA TTCCATTGTC GATTGAGCAC 
AAAACACTTC AGAAATTATC ACTTGAAACT GAACATTTGA CTTTAATGCA GAAGCTTCAA 
TTATTTGAAA GTGCTCTAAG TGTAACACAA GGAGAAGATC TCCGACATGT ATTATGGTTG 
AAGAGTCCGA GTTCTGAAGT TTGGTTTGAT CGACGAACCA ATTATACAAG ATCAGTTGCA 
TGTATGTCAA TGGTTGGATA TATTCTTGGT CTCGGTGACA GACATCCATC CAACTTGATG 
CTCGATCGAT TGACCGGAAA AGTTGTACAC ATTGATTTCG GTGACTGTTT CGAAGTTGCT 
ATGCTCCGTG AAAAATTCCC TGAACGAGTT CCATTCCGAC TTACCAGAAT GCTCATCAAC 
GCTATGGAAG TAACTGGATT GGACGGAGTA TACAATTACA CTGCCGAACG TGTCTTGAAA 
ATGTTGCGAA CAAATCAAGA ATCATTGTTA GCAGTTTTAG AAGCATTTGT CTATGATCCA 
GTAATTAATT GGCGTCTTGT GGAAGGAATG AAAAAGGATC CGAAAACTAG AAAAGATACA 
GGTGGACGTC AGAATATGGC TGGTGCTGTA TTACCATCAT CAAGTACAAC TGATTCAATT 
ATGGAGACGA TTAAGAGGAA ACTCGATGGG ACCGAATTCG TTCACACTGA CGGATCAACT 
CCACCTGAAC CACTTCAAGT AACTGAGCAA CTTGCAATGC TCACAGAGCA AGCAACATCT 
CCATTGAATT TGTGTCAAAG TTATATTGGA TGGTGTCCGT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-363 ( NM_059148.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491549.2 Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.

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CloneID	OCa336351
Clone ID Related Accession (Same CDS sequence)	NM_059148.5 , NM_059148.4
Accession Version	NM_059148.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Target of rapamycin homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059148.3. COMPLETENESS: incomplete on both ends.

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG TAATTATAGA TTACCCTGTT 
CCACCACCAC TTGGTTCACT CGTCGGTCTT CCACCAATGC CAGCTTATTT GTCTTCGAAT 
TCATCACTTC CACCACAACA TCATCATGTC TCACCATTGA GCAACGATTC TCCATCGAAT 
TCAGCTGAAA ATAAGCTTTA TTTGAAACAC GCGGCTCACG CTGTGCGATG CTTCGCAAAG 
GCTCTGATGT GCTCACCGGG TTCTCGACTT GAAGATACTC TCCGATTGAT GCAATTGTGG 
TTTGATCATG GAGATGATAA AGATCAAGAT GTTTATTTTG CTCTTACGGA GACAATCTTT 
GATCTTCCTG TGACAACATG GTTAGAAGCT ATTCCTCAAT TGATGGCTCG TTTGGATTGT 
CCAGATGATC AGAAAAGTGT TCAACTTGTT CTTCGAGTTC TTTGTGAAAT TGCCAGGCAT 
AGACCACAAG CAGTAATCTA TGCACTAACT GTTGCATCGA GAAGTAAGGA TGTACATAGA 
AGTAAGAATG CTGGAACAGT TCTCGAAAAA ATGATGGAAT ATCATTCAAA ATTGGTTCGA 
GAGGCAAGTC TTGTGACAGA AGAGCTTGTT CGTTGCGCAA TTCTGTGGCA TGAACAGTGG 
CATGATGCAC TTGATGATGC GAGCCGTGTG TATTTCCACG ATAACAATGT GCAGGCGATG 
TTTGATGCTT TGAGAAATAT GAATGATTTG ATGCAAAAAG GAGCTCCAAC AACCATGAAA 
GAGCACAGTT TCCAACAGAC GTATAGTTCC GATTTGAAAG AAGCAGGACG ATATGTACAA 
GCATTTGAGA GTAGTGGAAA TGTGAAAGAT CTTAATCAGG CGTGGGAGAT TTATTGTTCG 
GTTTTCAAAA AGCTGCGTGA TCAACTTGCC ACTTTGAACT CGTTGGATCT TGTCTACGTA 
TCACCTAATT TGGTCAGCGC AAAAGATTTG GAACTCGTTG TTCCTGGTAC ATACGATCCA 
TCAGCTCCAA TAGTTTCCAT TCAAAGTTTC AGCTCAAAAA TGAATGTAAT CACTAGCAAG 
CAACGTCCTC GTAAAATGGT GATCCGTGGA TCAAATGGTT TAGACTATCA ATTCTTGCTA 
AAAGGACATG AAGATCCGAG ACAAGATGAA CGTGTGATGC AACTTTTTGG ACTAGTCAAC 
ACACTTCTTG CCAACAATTC TGAGACTTGT CGCCGCAATT TGACTATTCA AAGATACTCG 
ATTGTAGCTT TGAGCAAAGA TTCTGGATTG ATTGGTTGGG TTCCAAATTG TGATACACTT 
CATACACTTG TCAAGGAATA TCGAGAAAAG AAAGCAAAGA TTCCATTGTC GATTGAGCAC 
AAAACACTTC AGAAATTATC ACTTGAAACT GAACATTTGA CTTTAATGCA GAAGCTTCAA 
TTATTTGAAA GTGCTCTAAG TGTAACACAA GGAGAAGATC TCCGACATGT ATTATGGTTG 
AAGAGTCCGA GTTCTGAAGT TTGGTTTGAT CGACGAACCA ATTATACAAG ATCAGTTGCA 
TGTATGTCAA TGGTTGGATA TATTCTTGGT CTCGGTGACA GACATCCATC CAACTTGATG 
CTCGATCGAT TGACCGGAAA AGTTGTACAC ATTGATTTCG GTGACTGTTT CGAAGTTGCT 
ATGCTCCGTG AAAAATTCCC TGAACGAGTT CCATTCCGAC TTACCAGAAT GCTCATCAAC 
GCTATGGAAG TAACTGGATT GGACGGAGTA TACAATTACA CTGCCGAACG TGTCTTGAAA 
ATGTTGCGAA CAAATCAAGA ATCATTGTTA GCAGTTTTAG AAGCATTTGT CTATGATCCA 
GTAATTAATT GGCGTCTTGT GGAAGGAATG AAAAAGGATC CGAAAACTAG AAAAGATACA 
GGTGGACGTC AGAATATGGC TGGTGCTGTA TTACCATCAT CAAGTACAAC TGATTCAATT 
ATGGAGACGA TTAAGAGGAA ACTCGATGGG ACCGAATTCG TTCACACTGA CGGATCAACT 
CCACCTGAAC CACTTCAAGT AACTGAGCAA CTTGCAATGC TCACAGAGCA AGCAACATCT 
CCATTGAATT TGTGTCAAAG TTATATTGGA TGGTGTCCGT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491549.2
CDS	1..8088
Translation	MLQQHGISFQMNADRQNKAATTSNRIVSAPATNEFDERHRKQGIDAARALASQYRSRITENRDANQRQAARELSRYVRSELKDEPNTFSDAFLNAIDGRTDIASQSAIYNCMKSNSNIDQKRAGIYLIVCLAETHSGNVIRYANYLLKMLNNGNGLDEDTVKMASKALAFLIATCKSYAAELVDRCLDHCHEWLGQNVPHSQQPKNQQEIDQIRRLAASHLSRELALATPTAFFLRVNLFFKYIFNAVRDKNPAVRIAGIDALHVVLTIVSQREAKNKTEWFKKCFDEALEGQPNPSQKDDLDRWHAVALILNELLRISDQRFELIRCESSQFIKQKFLKEDEEEGVEWLVLTKQQTIVESVTARKLVLERFPKILDCVRQIIPLANKTSSTKQQSSIYLNTVLMQLLPRICAFPQCDRTFQTISFDTSFTILQRNAVAAPAIGMMMLSNPDVHATHIEKTISFISAAIKKTTNSDVLDNYFTFLFLFVDAYHEQVTTQIKAIIPQLMDITLSRSLANVLKMIMMRIPKLRLNVQDGVMASVYQTLTGSLIPPKSEPIGRPASPKAILQKAETDPKELQRIVLAVDVLGEFYFSRGALQRIMQYVADYYLTADNVEIRLAAVSSCCEMVVPFVGVYKKVTSDKRNSLLQTIYGVLRAVCSVIVNDQDVRVRMQVISCFGQMPRPFLAHLAQPEMLEVQFMALHDEKLEMQQACVTLLGRLAELNPALVLPRLRLMLLETLSQMQQSGQARLEQHSAKMIAQLAKQSPKFMRPYVGSLMIAMIPKLRNDQKYAEVTAQVLNAVSEIAVIGGAEIVKNLKPLFEKLTHMINDSSSLHKREAALRAIGGICRSTAYVVDPYRDYPSLLDDLLRILKTVMSNTMRREAIKTLGILGAIDPYTHKVFTGSVQSSTAISTALSLPISETDSKDPRQDIIHWFNYEKCTLEEFYPAITIANLMLMMQDEDSQSYAEIAQAIVTIFRSLGDMAPLYTEQVIPRLIEVCRRATESSNRANLREFFLQQLANFVAIIRKHAAPYMPAIFTIIADAWKEDISVKMVVIEVLTDMGTAIGNDFSKYTGELIPYLLTVLQTDKTKERVLTVKVMESIQKLTHCIVQHLHLVLPPLLIILDDFSLKLSIRNTALSTVLHMTQQVDVSAYAPRMMQSWHHNISTAEMRDKLLLLLIEIIKQLGKFFDIFKRGVDQKLRDYNLDKSVHYEQYRKLAQRAQMSRDVLTSSVFAGSNGNIQYSSTQAGMRGQANNVYANNDLHERLMNGSIDSGASRQDNRDDYYRYGVEEKISEEVPKVAPTTARPTSELVTVQITKQRLNKDALMPQWKNENLTSKDEWLQWLMKIRIGFLTYGSSPSLRAASSLGDQHPHLARDLFPAAFMSVWTELDSDVQNDLTSCLLRAISTGIPELIQTILNLAEFMDHSEKGPLPISHDVLGRWAEQTKAFAKACRYKEMSVLKKSGSMQTTFTRKVKLEPNDCQSLITYANKLNVQEEAAGVVRYAERNEMNFQMRGRWYEKLNEWEKALGAYELEEKKKSSCPNLQVYDEKDHLMTPEEAATAEEARMHEMRCLEALGRWDELNSKSVVWADQRGNRNDSVRDEINKKQLDHKMAVIAARGAWAVDNWERMADYVSVISENTQDGAMLRAVVAVHNDENTKAMGLIEKVREMIDSELTAMANESYERAYIPMVSVQQMAELEEAIEYKTRPERRPRIALLWSRRLQGCRRNVEQWQRLIMLRGLVLSPQEMHPLRVKFSSMCRKQGKNSMSRAVLRELLSLPANSDLVRAKAPFDKPLLVLALAKQLYQDDHKDEAIRALEDLANHWNKRINPIPKATGRELIPPSTKEPARICAKVLLKLGEWTELKSKTSNNMQVGELSFVRQQVSPQYRTKESRTPETIAFENTINYYQQATQYDPGWHKVWHKLASTHFYAVCRERPHPTTVISPPQQPQQPKKMHIPPVTRATSPPPPAQKSPQPAPFHSITEPLSVIIDYPVPPPLGSLVGLPPMPAYLSSNSSLPPQHHHVSPLSNDSPSNSAENKLYLKHAAHAVRCFAKALMCSPGSRLEDTLRLMQLWFDHGDDKDQDVYFALTETIFDLPVTTWLEAIPQLMARLDCPDDQKSVQLVLRVLCEIARHRPQAVIYALTVASRSKDVHRSKNAGTVLEKMMEYHSKLVREASLVTEELVRCAILWHEQWHDALDDASRVYFHDNNVQAMFDALRNMNDLMQKGAPTTMKEHSFQQTYSSDLKEAGRYVQAFESSGNVKDLNQAWEIYCSVFKKLRDQLATLNSLDLVYVSPNLVSAKDLELVVPGTYDPSAPIVSIQSFSSKMNVITSKQRPRKMVIRGSNGLDYQFLLKGHEDPRQDERVMQLFGLVNTLLANNSETCRRNLTIQRYSIVALSKDSGLIGWVPNCDTLHTLVKEYREKKAKIPLSIEHKTLQKLSLETEHLTLMQKLQLFESALSVTQGEDLRHVLWLKSPSSEVWFDRRTNYTRSVACMSMVGYILGLGDRHPSNLMLDRLTGKVVHIDFGDCFEVAMLREKFPERVPFRLTRMLINAMEVTGLDGVYNYTAERVLKMLRTNQESLLAVLEAFVYDPVINWRLVEGMKKDPKTRKDTGGRQNMAGAVLPSSSTTDSIMETIKRKLDGTEFVHTDGSTPPEPLQVTEQLAMLTEQATSPLNLCQSYIGWCPFW

Target ORF information:

RefSeq Version	NM_059148.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059148.4

ORF Insert Sequence:

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG TAATTATAGA TTACCCTGTT 
CCACCACCAC TTGGTTCACT CGTCGGTCTT CCACCAATGC CAGCTTATTT GTCTTCGAAT 
TCATCACTTC CACCACAACA TCATCATGTC TCACCATTGA GCAACGATTC TCCATCGAAT 
TCAGCTGAAA ATAAGCTTTA TTTGAAACAC GCGGCTCACG CTGTGCGATG CTTCGCAAAG 
GCTCTGATGT GCTCACCGGG TTCTCGACTT GAAGATACTC TCCGATTGAT GCAATTGTGG 
TTTGATCATG GAGATGATAA AGATCAAGAT GTTTATTTTG CTCTTACGGA GACAATCTTT 
GATCTTCCTG TGACAACATG GTTAGAAGCT ATTCCTCAAT TGATGGCTCG TTTGGATTGT 
CCAGATGATC AGAAAAGTGT TCAACTTGTT CTTCGAGTTC TTTGTGAAAT TGCCAGGCAT 
AGACCACAAG CAGTAATCTA TGCACTAACT GTTGCATCGA GAAGTAAGGA TGTACATAGA 
AGTAAGAATG CTGGAACAGT TCTCGAAAAA ATGATGGAAT ATCATTCAAA ATTGGTTCGA 
GAGGCAAGTC TTGTGACAGA AGAGCTTGTT CGTTGCGCAA TTCTGTGGCA TGAACAGTGG 
CATGATGCAC TTGATGATGC GAGCCGTGTG TATTTCCACG ATAACAATGT GCAGGCGATG 
TTTGATGCTT TGAGAAATAT GAATGATTTG ATGCAAAAAG GAGCTCCAAC AACCATGAAA 
GAGCACAGTT TCCAACAGAC GTATAGTTCC GATTTGAAAG AAGCAGGACG ATATGTACAA 
GCATTTGAGA GTAGTGGAAA TGTGAAAGAT CTTAATCAGG CGTGGGAGAT TTATTGTTCG 
GTTTTCAAAA AGCTGCGTGA TCAACTTGCC ACTTTGAACT CGTTGGATCT TGTCTACGTA 
TCACCTAATT TGGTCAGCGC AAAAGATTTG GAACTCGTTG TTCCTGGTAC ATACGATCCA 
TCAGCTCCAA TAGTTTCCAT TCAAAGTTTC AGCTCAAAAA TGAATGTAAT CACTAGCAAG 
CAACGTCCTC GTAAAATGGT GATCCGTGGA TCAAATGGTT TAGACTATCA ATTCTTGCTA 
AAAGGACATG AAGATCCGAG ACAAGATGAA CGTGTGATGC AACTTTTTGG ACTAGTCAAC 
ACACTTCTTG CCAACAATTC TGAGACTTGT CGCCGCAATT TGACTATTCA AAGATACTCG 
ATTGTAGCTT TGAGCAAAGA TTCTGGATTG ATTGGTTGGG TTCCAAATTG TGATACACTT 
CATACACTTG TCAAGGAATA TCGAGAAAAG AAAGCAAAGA TTCCATTGTC GATTGAGCAC 
AAAACACTTC AGAAATTATC ACTTGAAACT GAACATTTGA CTTTAATGCA GAAGCTTCAA 
TTATTTGAAA GTGCTCTAAG TGTAACACAA GGAGAAGATC TCCGACATGT ATTATGGTTG 
AAGAGTCCGA GTTCTGAAGT TTGGTTTGAT CGACGAACCA ATTATACAAG ATCAGTTGCA 
TGTATGTCAA TGGTTGGATA TATTCTTGGT CTCGGTGACA GACATCCATC CAACTTGATG 
CTCGATCGAT TGACCGGAAA AGTTGTACAC ATTGATTTCG GTGACTGTTT CGAAGTTGCT 
ATGCTCCGTG AAAAATTCCC TGAACGAGTT CCATTCCGAC TTACCAGAAT GCTCATCAAC 
GCTATGGAAG TAACTGGATT GGACGGAGTA TACAATTACA CTGCCGAACG TGTCTTGAAA 
ATGTTGCGAA CAAATCAAGA ATCATTGTTA GCAGTTTTAG AAGCATTTGT CTATGATCCA 
GTAATTAATT GGCGTCTTGT GGAAGGAATG AAAAAGGATC CGAAAACTAG AAAAGATACA 
GGTGGACGTC AGAATATGGC TGGTGCTGTA TTACCATCAT CAAGTACAAC TGATTCAATT 
ATGGAGACGA TTAAGAGGAA ACTCGATGGG ACCGAATTCG TTCACACTGA CGGATCAACT 
CCACCTGAAC CACTTCAAGT AACTGAGCAA CTTGCAATGC TCACAGAGCA AGCAACATCT 
CCATTGAATT TGTGTCAAAG TTATATTGGA TGGTGTCCGT TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-363 ( NM_059151.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491552.2 Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.

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CloneID	OCa336352
Clone ID Related Accession (Same CDS sequence)	NM_059151.6 , NM_059151.5
Accession Version	NM_059151.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Target of rapamycin homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059151.5.

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG ATTACCCTGT TCCACCACCA 
CTTGGTTCAC TCGTCGGTCT TCCACCAATG CCAGCTTATT TGTCTTCGAA TTCATCACTT 
CCACCACAAC ATCATCATGT CTCACCATTG AGCAACGATT CTCCATCGAA TTCAGCTGAA 
AATAAGCTTT ATTTGAAACA CGCGGCTCAC GCTGTGCGAT GCTTCGCAAA GGCTCTGATG 
TGCTCACCGG GTTCTCGACT TGAAGATACT CTCCGATTGA TGCAATTGTG GTTTGATCAT 
GGAGATGATA AAGATCAAGA TGTTTATTTT GCTCTTACGG AGACAATCTT TGATCTTCCT 
GTGACAACAT GGTTAGAAGC TATTCCTCAA TTGATGGCTC GTTTGGATTG TCCAGATGAT 
CAGAAAAGTG TTCAACTTGT TCTTCGAGTT CTTTGTGAAA TTGCCAGGCA TAGACCACAA 
GCAGTAATCT ATGCACTAAC TGTTGCATCG AGAAGTAAGG ATGTACATAG AAGTAAGAAT 
GCTGGAACAG TTCTCGAAAA AATGATGGAA TATCATTCAA AATTGGTTCG AGAGGCAAGT 
CTTGTGACAG AAGAGCTTGT TCGTTGCGCA ATTCTGTGGC ATGAACAGTG GCATGATGCA 
CTTGATGATG CGAGCCGTGT GTATTTCCAC GATAACAATG TGCAGGCGAT GTTTGATGCT 
TTGAGAAATA TGAATGATTT GATGCAAAAA GGAGCTCCAA CAACCATGAA AGAGCACAGT 
TTCCAACAGA CGTATAGTTC CGATTTGAAA GAAGCAGGAC GATATGTACA AGCATTTGAG 
AGTAGTGGAA ATGTGAAAGA TCTTAATCAG GCGTGGGAGA TTTATTGTTC GGTTTTCAAA 
AAGCTGCGTG ATCAACTTGC CACTTTGAAC TCGTTGGATC TTGTCTACGT ATCACCTAAT 
TTGGTCAGCG CAAAAGATTT GGAACTCGTT GTTCCTGGTA CATACGATCC ATCAGCTCCA 
ATAGTTTCCA TTCAAAGTTT CAGCTCAAAA ATGAATGTAA TCACTAGCAA GCAACGTCCT 
CGTAAAATGG TGATCCGTGG ATCAAATGGT TTAGACTATC AATTCTTGCT AAAAGGACAT 
GAAGATCCGA GACAAGATGA ACGTGTGATG CAACTTTTTG GACTAGTCAA CACACTTCTT 
GCCAACAATT CTGAGACTTG TCGCCGCAAT TTGACTATTC AAAGATACTC GATTGTAGCT 
TTGAGCAAAG ATTCTGGATT GATTGGTTGG GTTCCAAATT GTGATACACT TCATACACTT 
GTCAAGGAAT ATCGAGAAAA GAAAGCAAAG ATTCCATTGT CGATTGAGCA CAAAACACTT 
CAGAAATTAT CACTTGAAAC TGAACATTTG ACTTTAATGC AGAAGCTTCA ATTATTTGAA 
AGTGCTCTAA GTGTAACACA AGGAGAAGAT CTCCGACATG TATTATGGTT GAAGAGTCCG 
AGTTCTGAAG TTTGGTTTGA TCGACGAACC AATTATACAA GATCAGTTGC ATGTATGTCA 
ATGGTTGGAT ATATTCTTGG TCTCGGTGAC AGACATCCAT CCAACTTGAT GCTCGATCGA 
TTGACCGGAA AAGTTGTACA CATTGATTTC GGTGACTGTT TCGAAGTTGC TATGCTCCGT 
GAAAAATTCC CTGAACGAGT TCCATTCCGA CTTACCAGAA TGCTCATCAA CGCTATGGAA 
GTAACTGGAT TGGACGGAGT ATACAATTAC ACTGCCGAAC GTGTCTTGAA AATGTTGCGA 
ACAAATCAAG AATCATTGTT AGCAGTTTTA GAAGCATTTG TCTATGATCC AGTAATTAAT 
TGGCGTCTTG TGGAAGGAAT GAAAAAGGAT CCGAAAACTA GAAAAGATAC AGGTGGACGT 
CAGAATATGG CTGGTGCTGT ATTACCATCA TCAAGTACAA CTGATTCAAT TATGGAGACG 
ATTAAGAGGA AACTCGATGG GACCGAATTC GTTCACACTG ACGGATCAAC TCCACCTGAA 
CCACTTCAAG TAACTGAGCA ACTTGCAATG CTCACAGAGC AAGCAACATC TCCATTGAAT 
TTGTGTCAAA GTTATATTGG ATGGTGTCCG TTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491552.2
CDS	39..8117
Translation	MLQQHGISFQMNADRQNKAATTSNRIVSAPATNEFDERHRKQGIDAARALASQYRSRITENRDANQRQAARELSRYVRSELKDEPNTFSDAFLNAIDGRTDIASQSAIYNCMKSNSNIDQKRAGIYLIVCLAETHSGNVIRYANYLLKMLNNGNGLDEDTVKMASKALAFLIATCKSYAAELVDRCLDHCHEWLGQNVPHSQQPKNQQEIDQIRRLAASHLSRELALATPTAFFLRVNLFFKYIFNAVRDKNPAVRIAGIDALHVVLTIVSQREAKNKTEWFKKCFDEALEGQPNPSQKDDLDRWHAVALILNELLRISDQRFELIRCESSQFIKQKFLKEDEEEGVEWLVLTKQQTIVESVTARKLVLERFPKILDCVRQIIPLANKTSSTKQQSSIYLNTVLMQLLPRICAFPQCDRTFQTISFDTSFTILQRNAVAAPAIGMMMLSNPDVHATHIEKTISFISAAIKKTTNSDVLDNYFTFLFLFVDAYHEQVTTQIKAIIPQLMDITLSRSLANVLKMIMMRIPKLRLNVQDGVMASVYQTLTGSLIPPKSEPIGRPASPKAILQKAETDPKELQRIVLAVDVLGEFYFSRGALQRIMQYVADYYLTADNVEIRLAAVSSCCEMVVPFVGVYKKVTSDKRNSLLQTIYGVLRAVCSVIVNDQDVRVRMQVISCFGQMPRPFLAHLAQPEMLEVQFMALHDEKLEMQQACVTLLGRLAELNPALVLPRLRLMLLETLSQMQQSGQARLEQHSAKMIAQLAKQSPKFMRPYVGSLMIAMIPKLRNDQKYAEVTAQVLNAVSEIAVIGGAEIVKNLKPLFEKLTHMINDSSSLHKREAALRAIGGICRSTAYVVDPYRDYPSLLDDLLRILKTVMSNTMRREAIKTLGILGAIDPYTHKVFTGSVQSSTAISTALSLPISETDSKDPRQDIIHWFNYEKCTLEEFYPAITIANLMLMMQDEDSQSYAEIAQAIVTIFRSLGDMAPLYTEQVIPRLIEVCRRATESSNRANLREFFLQQLANFVAIIRKHAAPYMPAIFTIIADAWKEDISVKMVVIEVLTDMGTAIGNDFSKYTGELIPYLLTVLQTDKTKERVLTVKVMESIQKLTHCIVQHLHLVLPPLLIILDDFSLKLSIRNTALSTVLHMTQQVDVSAYAPRMMQSWHHNISTAEMRDKLLLLLIEIIKQLGKFFDIFKRGVDQKLRDYNLDKSVHYEQYRKLAQRAQMSRDVLTSSVFAGSNGNIQYSSTQAGMRGQANNVYANNDLHERLMNGSIDSGASRQDNRDDYYRYGVEEKISEEVPKVAPTTARPTSELVTVQITKQRLNKDALMPQWKNENLTSKDEWLQWLMKIRIGFLTYGSSPSLRAASSLGDQHPHLARDLFPAAFMSVWTELDSDVQNDLTSCLLRAISTGIPELIQTILNLAEFMDHSEKGPLPISHDVLGRWAEQTKAFAKACRYKEMSVLKKSGSMQTTFTRKVKLEPNDCQSLITYANKLNVQEEAAGVVRYAERNEMNFQMRGRWYEKLNEWEKALGAYELEEKKKSSCPNLQVYDEKDHLMTPEEAATAEEARMHEMRCLEALGRWDELNSKSVVWADQRGNRNDSVRDEINKKQLDHKMAVIAARGAWAVDNWERMADYVSVISENTQDGAMLRAVVAVHNDENTKAMGLIEKVREMIDSELTAMANESYERAYIPMVSVQQMAELEEAIEYKTRPERRPRIALLWSRRLQGCRRNVEQWQRLIMLRGLVLSPQEMHPLRVKFSSMCRKQGKNSMSRAVLRELLSLPANSDLVRAKAPFDKPLLVLALAKQLYQDDHKDEAIRALEDLANHWNKRINPIPKATGRELIPPSTKEPARICAKVLLKLGEWTELKSKTSNNMQVGELSFVRQQVSPQYRTKESRTPETIAFENTINYYQQATQYDPGWHKVWHKLASTHFYAVCRERPHPTTVISPPQQPQQPKKMHIPPVTRATSPPPPAQKSPQPAPFHSITEPLSDYPVPPPLGSLVGLPPMPAYLSSNSSLPPQHHHVSPLSNDSPSNSAENKLYLKHAAHAVRCFAKALMCSPGSRLEDTLRLMQLWFDHGDDKDQDVYFALTETIFDLPVTTWLEAIPQLMARLDCPDDQKSVQLVLRVLCEIARHRPQAVIYALTVASRSKDVHRSKNAGTVLEKMMEYHSKLVREASLVTEELVRCAILWHEQWHDALDDASRVYFHDNNVQAMFDALRNMNDLMQKGAPTTMKEHSFQQTYSSDLKEAGRYVQAFESSGNVKDLNQAWEIYCSVFKKLRDQLATLNSLDLVYVSPNLVSAKDLELVVPGTYDPSAPIVSIQSFSSKMNVITSKQRPRKMVIRGSNGLDYQFLLKGHEDPRQDERVMQLFGLVNTLLANNSETCRRNLTIQRYSIVALSKDSGLIGWVPNCDTLHTLVKEYREKKAKIPLSIEHKTLQKLSLETEHLTLMQKLQLFESALSVTQGEDLRHVLWLKSPSSEVWFDRRTNYTRSVACMSMVGYILGLGDRHPSNLMLDRLTGKVVHIDFGDCFEVAMLREKFPERVPFRLTRMLINAMEVTGLDGVYNYTAERVLKMLRTNQESLLAVLEAFVYDPVINWRLVEGMKKDPKTRKDTGGRQNMAGAVLPSSSTTDSIMETIKRKLDGTEFVHTDGSTPPEPLQVTEQLAMLTEQATSPLNLCQSYIGWCPFW

Target ORF information:

RefSeq Version	NM_059151.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Target of rapamycin homolog (let-363), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059151.6

ORF Insert Sequence:

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG ATTACCCTGT TCCACCACCA 
CTTGGTTCAC TCGTCGGTCT TCCACCAATG CCAGCTTATT TGTCTTCGAA TTCATCACTT 
CCACCACAAC ATCATCATGT CTCACCATTG AGCAACGATT CTCCATCGAA TTCAGCTGAA 
AATAAGCTTT ATTTGAAACA CGCGGCTCAC GCTGTGCGAT GCTTCGCAAA GGCTCTGATG 
TGCTCACCGG GTTCTCGACT TGAAGATACT CTCCGATTGA TGCAATTGTG GTTTGATCAT 
GGAGATGATA AAGATCAAGA TGTTTATTTT GCTCTTACGG AGACAATCTT TGATCTTCCT 
GTGACAACAT GGTTAGAAGC TATTCCTCAA TTGATGGCTC GTTTGGATTG TCCAGATGAT 
CAGAAAAGTG TTCAACTTGT TCTTCGAGTT CTTTGTGAAA TTGCCAGGCA TAGACCACAA 
GCAGTAATCT ATGCACTAAC TGTTGCATCG AGAAGTAAGG ATGTACATAG AAGTAAGAAT 
GCTGGAACAG TTCTCGAAAA AATGATGGAA TATCATTCAA AATTGGTTCG AGAGGCAAGT 
CTTGTGACAG AAGAGCTTGT TCGTTGCGCA ATTCTGTGGC ATGAACAGTG GCATGATGCA 
CTTGATGATG CGAGCCGTGT GTATTTCCAC GATAACAATG TGCAGGCGAT GTTTGATGCT 
TTGAGAAATA TGAATGATTT GATGCAAAAA GGAGCTCCAA CAACCATGAA AGAGCACAGT 
TTCCAACAGA CGTATAGTTC CGATTTGAAA GAAGCAGGAC GATATGTACA AGCATTTGAG 
AGTAGTGGAA ATGTGAAAGA TCTTAATCAG GCGTGGGAGA TTTATTGTTC GGTTTTCAAA 
AAGCTGCGTG ATCAACTTGC CACTTTGAAC TCGTTGGATC TTGTCTACGT ATCACCTAAT 
TTGGTCAGCG CAAAAGATTT GGAACTCGTT GTTCCTGGTA CATACGATCC ATCAGCTCCA 
ATAGTTTCCA TTCAAAGTTT CAGCTCAAAA ATGAATGTAA TCACTAGCAA GCAACGTCCT 
CGTAAAATGG TGATCCGTGG ATCAAATGGT TTAGACTATC AATTCTTGCT AAAAGGACAT 
GAAGATCCGA GACAAGATGA ACGTGTGATG CAACTTTTTG GACTAGTCAA CACACTTCTT 
GCCAACAATT CTGAGACTTG TCGCCGCAAT TTGACTATTC AAAGATACTC GATTGTAGCT 
TTGAGCAAAG ATTCTGGATT GATTGGTTGG GTTCCAAATT GTGATACACT TCATACACTT 
GTCAAGGAAT ATCGAGAAAA GAAAGCAAAG ATTCCATTGT CGATTGAGCA CAAAACACTT 
CAGAAATTAT CACTTGAAAC TGAACATTTG ACTTTAATGC AGAAGCTTCA ATTATTTGAA 
AGTGCTCTAA GTGTAACACA AGGAGAAGAT CTCCGACATG TATTATGGTT GAAGAGTCCG 
AGTTCTGAAG TTTGGTTTGA TCGACGAACC AATTATACAA GATCAGTTGC ATGTATGTCA 
ATGGTTGGAT ATATTCTTGG TCTCGGTGAC AGACATCCAT CCAACTTGAT GCTCGATCGA 
TTGACCGGAA AAGTTGTACA CATTGATTTC GGTGACTGTT TCGAAGTTGC TATGCTCCGT 
GAAAAATTCC CTGAACGAGT TCCATTCCGA CTTACCAGAA TGCTCATCAA CGCTATGGAA 
GTAACTGGAT TGGACGGAGT ATACAATTAC ACTGCCGAAC GTGTCTTGAA AATGTTGCGA 
ACAAATCAAG AATCATTGTT AGCAGTTTTA GAAGCATTTG TCTATGATCC AGTAATTAAT 
TGGCGTCTTG TGGAAGGAAT GAAAAAGGAT CCGAAAACTA GAAAAGATAC AGGTGGACGT 
CAGAATATGG CTGGTGCTGT ATTACCATCA TCAAGTACAA CTGATTCAAT TATGGAGACG 
ATTAAGAGGA AACTCGATGG GACCGAATTC GTTCACACTG ACGGATCAAC TCCACCTGAA 
CCACTTCAAG TAACTGAGCA ACTTGCAATG CTCACAGAGC AAGCAACATC TCCATTGAAT 
TTGTGTCAAA GTTATATTGG ATGGTGTCCG TTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-363 ( NM_059151.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491552.2 Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.

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CloneID	OCa336352
Clone ID Related Accession (Same CDS sequence)	NM_059151.6 , NM_059151.5
Accession Version	NM_059151.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Target of rapamycin homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059151.4. COMPLETENESS: incomplete on both ends.

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG ATTACCCTGT TCCACCACCA 
CTTGGTTCAC TCGTCGGTCT TCCACCAATG CCAGCTTATT TGTCTTCGAA TTCATCACTT 
CCACCACAAC ATCATCATGT CTCACCATTG AGCAACGATT CTCCATCGAA TTCAGCTGAA 
AATAAGCTTT ATTTGAAACA CGCGGCTCAC GCTGTGCGAT GCTTCGCAAA GGCTCTGATG 
TGCTCACCGG GTTCTCGACT TGAAGATACT CTCCGATTGA TGCAATTGTG GTTTGATCAT 
GGAGATGATA AAGATCAAGA TGTTTATTTT GCTCTTACGG AGACAATCTT TGATCTTCCT 
GTGACAACAT GGTTAGAAGC TATTCCTCAA TTGATGGCTC GTTTGGATTG TCCAGATGAT 
CAGAAAAGTG TTCAACTTGT TCTTCGAGTT CTTTGTGAAA TTGCCAGGCA TAGACCACAA 
GCAGTAATCT ATGCACTAAC TGTTGCATCG AGAAGTAAGG ATGTACATAG AAGTAAGAAT 
GCTGGAACAG TTCTCGAAAA AATGATGGAA TATCATTCAA AATTGGTTCG AGAGGCAAGT 
CTTGTGACAG AAGAGCTTGT TCGTTGCGCA ATTCTGTGGC ATGAACAGTG GCATGATGCA 
CTTGATGATG CGAGCCGTGT GTATTTCCAC GATAACAATG TGCAGGCGAT GTTTGATGCT 
TTGAGAAATA TGAATGATTT GATGCAAAAA GGAGCTCCAA CAACCATGAA AGAGCACAGT 
TTCCAACAGA CGTATAGTTC CGATTTGAAA GAAGCAGGAC GATATGTACA AGCATTTGAG 
AGTAGTGGAA ATGTGAAAGA TCTTAATCAG GCGTGGGAGA TTTATTGTTC GGTTTTCAAA 
AAGCTGCGTG ATCAACTTGC CACTTTGAAC TCGTTGGATC TTGTCTACGT ATCACCTAAT 
TTGGTCAGCG CAAAAGATTT GGAACTCGTT GTTCCTGGTA CATACGATCC ATCAGCTCCA 
ATAGTTTCCA TTCAAAGTTT CAGCTCAAAA ATGAATGTAA TCACTAGCAA GCAACGTCCT 
CGTAAAATGG TGATCCGTGG ATCAAATGGT TTAGACTATC AATTCTTGCT AAAAGGACAT 
GAAGATCCGA GACAAGATGA ACGTGTGATG CAACTTTTTG GACTAGTCAA CACACTTCTT 
GCCAACAATT CTGAGACTTG TCGCCGCAAT TTGACTATTC AAAGATACTC GATTGTAGCT 
TTGAGCAAAG ATTCTGGATT GATTGGTTGG GTTCCAAATT GTGATACACT TCATACACTT 
GTCAAGGAAT ATCGAGAAAA GAAAGCAAAG ATTCCATTGT CGATTGAGCA CAAAACACTT 
CAGAAATTAT CACTTGAAAC TGAACATTTG ACTTTAATGC AGAAGCTTCA ATTATTTGAA 
AGTGCTCTAA GTGTAACACA AGGAGAAGAT CTCCGACATG TATTATGGTT GAAGAGTCCG 
AGTTCTGAAG TTTGGTTTGA TCGACGAACC AATTATACAA GATCAGTTGC ATGTATGTCA 
ATGGTTGGAT ATATTCTTGG TCTCGGTGAC AGACATCCAT CCAACTTGAT GCTCGATCGA 
TTGACCGGAA AAGTTGTACA CATTGATTTC GGTGACTGTT TCGAAGTTGC TATGCTCCGT 
GAAAAATTCC CTGAACGAGT TCCATTCCGA CTTACCAGAA TGCTCATCAA CGCTATGGAA 
GTAACTGGAT TGGACGGAGT ATACAATTAC ACTGCCGAAC GTGTCTTGAA AATGTTGCGA 
ACAAATCAAG AATCATTGTT AGCAGTTTTA GAAGCATTTG TCTATGATCC AGTAATTAAT 
TGGCGTCTTG TGGAAGGAAT GAAAAAGGAT CCGAAAACTA GAAAAGATAC AGGTGGACGT 
CAGAATATGG CTGGTGCTGT ATTACCATCA TCAAGTACAA CTGATTCAAT TATGGAGACG 
ATTAAGAGGA AACTCGATGG GACCGAATTC GTTCACACTG ACGGATCAAC TCCACCTGAA 
CCACTTCAAG TAACTGAGCA ACTTGCAATG CTCACAGAGC AAGCAACATC TCCATTGAAT 
TTGTGTCAAA GTTATATTGG ATGGTGTCCG TTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491552.2
CDS	1..8079
Translation	MLQQHGISFQMNADRQNKAATTSNRIVSAPATNEFDERHRKQGIDAARALASQYRSRITENRDANQRQAARELSRYVRSELKDEPNTFSDAFLNAIDGRTDIASQSAIYNCMKSNSNIDQKRAGIYLIVCLAETHSGNVIRYANYLLKMLNNGNGLDEDTVKMASKALAFLIATCKSYAAELVDRCLDHCHEWLGQNVPHSQQPKNQQEIDQIRRLAASHLSRELALATPTAFFLRVNLFFKYIFNAVRDKNPAVRIAGIDALHVVLTIVSQREAKNKTEWFKKCFDEALEGQPNPSQKDDLDRWHAVALILNELLRISDQRFELIRCESSQFIKQKFLKEDEEEGVEWLVLTKQQTIVESVTARKLVLERFPKILDCVRQIIPLANKTSSTKQQSSIYLNTVLMQLLPRICAFPQCDRTFQTISFDTSFTILQRNAVAAPAIGMMMLSNPDVHATHIEKTISFISAAIKKTTNSDVLDNYFTFLFLFVDAYHEQVTTQIKAIIPQLMDITLSRSLANVLKMIMMRIPKLRLNVQDGVMASVYQTLTGSLIPPKSEPIGRPASPKAILQKAETDPKELQRIVLAVDVLGEFYFSRGALQRIMQYVADYYLTADNVEIRLAAVSSCCEMVVPFVGVYKKVTSDKRNSLLQTIYGVLRAVCSVIVNDQDVRVRMQVISCFGQMPRPFLAHLAQPEMLEVQFMALHDEKLEMQQACVTLLGRLAELNPALVLPRLRLMLLETLSQMQQSGQARLEQHSAKMIAQLAKQSPKFMRPYVGSLMIAMIPKLRNDQKYAEVTAQVLNAVSEIAVIGGAEIVKNLKPLFEKLTHMINDSSSLHKREAALRAIGGICRSTAYVVDPYRDYPSLLDDLLRILKTVMSNTMRREAIKTLGILGAIDPYTHKVFTGSVQSSTAISTALSLPISETDSKDPRQDIIHWFNYEKCTLEEFYPAITIANLMLMMQDEDSQSYAEIAQAIVTIFRSLGDMAPLYTEQVIPRLIEVCRRATESSNRANLREFFLQQLANFVAIIRKHAAPYMPAIFTIIADAWKEDISVKMVVIEVLTDMGTAIGNDFSKYTGELIPYLLTVLQTDKTKERVLTVKVMESIQKLTHCIVQHLHLVLPPLLIILDDFSLKLSIRNTALSTVLHMTQQVDVSAYAPRMMQSWHHNISTAEMRDKLLLLLIEIIKQLGKFFDIFKRGVDQKLRDYNLDKSVHYEQYRKLAQRAQMSRDVLTSSVFAGSNGNIQYSSTQAGMRGQANNVYANNDLHERLMNGSIDSGASRQDNRDDYYRYGVEEKISEEVPKVAPTTARPTSELVTVQITKQRLNKDALMPQWKNENLTSKDEWLQWLMKIRIGFLTYGSSPSLRAASSLGDQHPHLARDLFPAAFMSVWTELDSDVQNDLTSCLLRAISTGIPELIQTILNLAEFMDHSEKGPLPISHDVLGRWAEQTKAFAKACRYKEMSVLKKSGSMQTTFTRKVKLEPNDCQSLITYANKLNVQEEAAGVVRYAERNEMNFQMRGRWYEKLNEWEKALGAYELEEKKKSSCPNLQVYDEKDHLMTPEEAATAEEARMHEMRCLEALGRWDELNSKSVVWADQRGNRNDSVRDEINKKQLDHKMAVIAARGAWAVDNWERMADYVSVISENTQDGAMLRAVVAVHNDENTKAMGLIEKVREMIDSELTAMANESYERAYIPMVSVQQMAELEEAIEYKTRPERRPRIALLWSRRLQGCRRNVEQWQRLIMLRGLVLSPQEMHPLRVKFSSMCRKQGKNSMSRAVLRELLSLPANSDLVRAKAPFDKPLLVLALAKQLYQDDHKDEAIRALEDLANHWNKRINPIPKATGRELIPPSTKEPARICAKVLLKLGEWTELKSKTSNNMQVGELSFVRQQVSPQYRTKESRTPETIAFENTINYYQQATQYDPGWHKVWHKLASTHFYAVCRERPHPTTVISPPQQPQQPKKMHIPPVTRATSPPPPAQKSPQPAPFHSITEPLSDYPVPPPLGSLVGLPPMPAYLSSNSSLPPQHHHVSPLSNDSPSNSAENKLYLKHAAHAVRCFAKALMCSPGSRLEDTLRLMQLWFDHGDDKDQDVYFALTETIFDLPVTTWLEAIPQLMARLDCPDDQKSVQLVLRVLCEIARHRPQAVIYALTVASRSKDVHRSKNAGTVLEKMMEYHSKLVREASLVTEELVRCAILWHEQWHDALDDASRVYFHDNNVQAMFDALRNMNDLMQKGAPTTMKEHSFQQTYSSDLKEAGRYVQAFESSGNVKDLNQAWEIYCSVFKKLRDQLATLNSLDLVYVSPNLVSAKDLELVVPGTYDPSAPIVSIQSFSSKMNVITSKQRPRKMVIRGSNGLDYQFLLKGHEDPRQDERVMQLFGLVNTLLANNSETCRRNLTIQRYSIVALSKDSGLIGWVPNCDTLHTLVKEYREKKAKIPLSIEHKTLQKLSLETEHLTLMQKLQLFESALSVTQGEDLRHVLWLKSPSSEVWFDRRTNYTRSVACMSMVGYILGLGDRHPSNLMLDRLTGKVVHIDFGDCFEVAMLREKFPERVPFRLTRMLINAMEVTGLDGVYNYTAERVLKMLRTNQESLLAVLEAFVYDPVINWRLVEGMKKDPKTRKDTGGRQNMAGAVLPSSSTTDSIMETIKRKLDGTEFVHTDGSTPPEPLQVTEQLAMLTEQATSPLNLCQSYIGWCPFW

Target ORF information:

RefSeq Version	NM_059151.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Target of rapamycin homolog (let-363), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059151.5

ORF Insert Sequence:

ATGCTCCAAC AACACGGAAT TAGTTTTCAA ATGAATGCCG ATAGACAGAA CAAAGCAGCC 
ACAACTTCCA ATCGAATAGT CTCCGCTCCA GCTACAAATG AATTTGACGA GCGGCATCGT 
AAACAAGGGA TTGATGCTGC AAGAGCTCTT GCTTCACAAT ATCGGAGCCG AATTACGGAA 
AATAGAGATG CAAATCAACG ACAGGCTGCT CGAGAACTCA GCAGATATGT TCGAAGTGAG 
CTGAAAGACG AGCCAAATAC TTTTAGTGAT GCTTTCCTGA ATGCAATCGA TGGACGAACA 
GATATAGCCT CTCAGAGTGC AATTTATAAT TGTATGAAGT CGAATTCAAA TATCGATCAG 
AAACGAGCCG GTATCTATCT CATCGTGTGC TTAGCTGAAA CTCATTCTGG AAATGTTATT 
CGATATGCGA ATTATCTTCT GAAAATGTTA AATAATGGAA ATGGATTAGA TGAAGATACA 
GTCAAGATGG CTTCAAAAGC TCTCGCTTTT CTGATTGCGA CTTGTAAATC ATACGCGGCC 
GAGCTGGTTG ATCGTTGTCT TGATCATTGC CACGAATGGC TTGGTCAAAA TGTTCCACAT 
AGTCAGCAAC CCAAAAATCA ACAGGAAATT GATCAAATTC GTCGACTCGC CGCTTCACAC 
CTCTCTCGTG AGCTCGCACT GGCCACACCG ACAGCATTCT TTCTTCGTGT AAACTTATTT 
TTCAAGTATA TCTTCAACGC TGTCAGAGAC AAGAATCCAG CTGTAAGAAT CGCCGGAATT 
GATGCATTAC ACGTGGTTTT GACAATTGTT TCACAGCGTG AAGCAAAGAA TAAAACTGAG 
TGGTTTAAGA AATGTTTCGA TGAAGCACTG GAAGGTCAAC CAAACCCATC GCAAAAGGAC 
GATTTGGATA GATGGCATGC CGTCGCCCTG ATTCTCAATG AACTTCTTCG AATTTCTGAT 
CAACGATTCG AACTCATCAG ATGTGAATCC TCACAGTTTA TTAAACAAAA GTTTTTGAAA 
GAAGACGAAG AAGAAGGTGT TGAATGGCTT GTCCTAACTA AGCAACAAAC TATTGTGGAA 
AGTGTGACTG CTCGAAAGCT GGTTTTGGAA CGATTTCCGA AGATACTCGA TTGTGTTCGT 
CAGATAATAC CTCTCGCAAA CAAAACTTCA TCCACGAAAC AGCAAAGCTC GATTTACCTG 
AATACAGTTC TCATGCAGCT TCTACCAAGA ATTTGTGCAT TTCCACAATG TGATCGAACT 
TTTCAAACAA TCTCATTCGA CACTTCATTT ACTATTCTTC AAAGAAATGC GGTCGCTGCT 
CCAGCAATCG GAATGATGAT GCTCTCGAAT CCAGATGTAC ATGCCACACA TATCGAGAAA 
ACAATCAGTT TTATCTCTGC TGCTATCAAA AAAACGACGA ATTCTGATGT TCTCGATAAT 
TATTTCACAT TCCTGTTCCT ATTTGTGGAT GCTTATCACG AACAAGTGAC GACACAAATC 
AAGGCAATAA TTCCACAATT AATGGATATT ACCCTGTCTC GATCGCTGGC AAACGTGCTA 
AAAATGATTA TGATGAGAAT TCCGAAACTG CGGCTGAATG TTCAAGATGG TGTTATGGCT 
TCTGTCTATC AAACACTCAC TGGATCTCTA ATTCCACCAA AATCCGAACC GATTGGAAGG 
CCTGCGAGTC CTAAAGCTAT TTTGCAGAAA GCAGAAACCG ATCCAAAAGA GCTTCAACGA 
ATCGTGCTGG CTGTCGATGT GCTCGGTGAA TTCTATTTCT CAAGAGGTGC CTTGCAGAGA 
ATCATGCAAT ACGTTGCAGA TTACTACTTG ACAGCGGACA ATGTGGAAAT CCGTTTGGCT 
GCTGTCTCTT CGTGTTGTGA AATGGTCGTA CCATTTGTTG GAGTTTATAA AAAAGTTACA 
AGTGACAAGA GAAATAGTCT TCTTCAAACT ATTTATGGTG TGCTGCGAGC TGTTTGTTCG 
GTTATTGTTA ATGATCAAGA TGTCCGTGTC AGAATGCAAG TAATCAGCTG TTTCGGACAA 
ATGCCACGGC CATTCCTTGC TCATTTGGCT CAACCCGAAA TGCTCGAAGT ACAATTTATG 
GCTCTTCACG ACGAAAAACT CGAAATGCAG CAAGCATGTG TGACGTTACT CGGACGACTC 
GCAGAGCTCA ATCCGGCACT TGTTTTGCCA CGTCTTCGAT TGATGCTCCT AGAGACATTG 
AGTCAAATGC AACAAAGTGG TCAGGCAAGA TTGGAGCAAC ATTCTGCGAA AATGATCGCG 
CAATTGGCGA AACAGAGTCC AAAGTTCATG AGACCATACG TTGGAAGTTT AATGATTGCT 
ATGATTCCAA AATTGAGAAA TGATCAAAAA TATGCCGAAG TGACTGCCCA AGTTCTGAAT 
GCTGTCAGCG AAATCGCCGT GATTGGTGGA GCCGAAATAG TAAAAAATTT GAAGCCATTG 
TTCGAAAAGT TGACACATAT GATAAATGAT TCGAGCAGCT TGCATAAACG AGAGGCGGCT 
CTCCGTGCAA TTGGTGGAAT ATGTCGTTCA ACTGCATATG TTGTGGATCC ATATCGAGAC 
TATCCAAGTC TACTTGATGA TCTTCTTCGA ATCCTGAAAA CTGTCATGTC AAATACAATG 
AGACGAGAAG CTATCAAAAC TCTCGGAATT CTTGGAGCAA TCGATCCATA TACTCATAAA 
GTATTCACTG GCTCAGTACA AAGCTCGACA GCTATAAGCA CAGCCTTATC ACTTCCAATC 
TCTGAAACTG ATTCAAAAGA TCCACGTCAA GACATTATTC ATTGGTTTAA CTATGAAAAA 
TGTACATTAG AAGAGTTTTA TCCAGCGATT ACGATTGCAA ATTTGATGTT AATGATGCAA 
GACGAAGATT CGCAGAGCTA CGCGGAAATT GCTCAAGCTA TAGTAACAAT CTTCAGAAGT 
CTTGGTGATA TGGCACCATT GTATACAGAA CAAGTCATTC CAAGGCTAAT AGAAGTTTGT 
CGGCGAGCAA CTGAATCATC AAATCGAGCC AATTTGAGAG AGTTCTTTCT TCAACAATTG 
GCAAATTTCG TGGCAATTAT AAGAAAGCAC GCGGCACCGT ATATGCCAGC TATTTTCACG 
ATTATCGCGG ATGCTTGGAA AGAGGATATA TCCGTCAAAA TGGTGGTCAT TGAAGTTCTC 
ACTGATATGG GAACTGCCAT TGGAAACGAT TTCAGTAAAT ACACAGGCGA ACTAATACCG 
TATCTGTTGA CTGTACTACA AACTGATAAG ACCAAGGAAC GAGTTCTCAC TGTGAAGGTA 
ATGGAATCAA TTCAAAAACT GACTCATTGT ATAGTTCAAC ATCTTCATCT TGTTCTTCCG 
CCTCTTCTCA TAATTCTTGA TGATTTCTCG TTGAAGCTAT CAATTCGAAA CACGGCTCTT 
TCTACAGTAC TTCATATGAC TCAACAAGTT GATGTGTCTG CATATGCTCC ACGTATGATG 
CAGAGTTGGC ATCATAATAT TTCAACTGCT GAAATGAGAG ATAAACTGCT CCTTTTATTG 
ATTGAAATCA TCAAGCAACT CGGAAAATTC TTTGATATTT TCAAACGAGG CGTTGATCAG 
AAGCTACGCG ATTATAATCT TGATAAAAGT GTTCACTATG AGCAGTATCG GAAGCTTGCT 
CAACGAGCAC AAATGTCACG TGATGTGCTT ACTTCATCAG TTTTTGCGGG ATCCAATGGA 
AATATACAGT ACTCTTCGAC GCAAGCGGGC ATGCGAGGTC AGGCTAACAA TGTTTATGCA 
AATAATGATC TACACGAGAG ACTGATGAAT GGATCAATTG ATTCGGGAGC GAGTCGACAG 
GACAATCGAG ATGATTATTA TAGATATGGA GTCGAGGAGA AAATTTCAGA AGAAGTTCCA 
AAAGTTGCTC CAACAACTGC TCGCCCTACC AGTGAACTCG TGACGGTTCA AATCACAAAA 
CAACGTCTCA ATAAGGATGC TTTGATGCCA CAATGGAAAA ATGAGAATTT GACTTCAAAA 
GATGAATGGT TGCAGTGGTT GATGAAGATT CGAATAGGAT TTTTAACTTA TGGAAGTTCT 
CCATCGTTAC GTGCAGCTTC TTCACTTGGT GATCAACATC CTCATCTTGC ACGTGATCTA 
TTTCCAGCTG CTTTCATGTC TGTATGGACT GAACTTGATT CGGATGTACA AAACGATCTA 
ACTTCATGTC TTCTCCGAGC AATCTCCACA GGAATTCCAG AATTAATTCA AACGATTCTG 
AATCTTGCCG AGTTTATGGA TCATTCTGAG AAAGGACCAC TTCCAATTTC TCATGATGTT 
CTCGGTCGAT GGGCTGAACA AACGAAGGCT TTTGCCAAAG CATGTAGATA TAAGGAGATG 
AGTGTACTGA AGAAGTCGGG TTCAATGCAA ACGACGTTTA CGAGAAAAGT GAAGCTGGAG 
CCAAATGATT GTCAGAGCCT TATCACATAT GCCAATAAAT TGAACGTACA AGAGGAAGCT 
GCAGGTGTCG TAAGATATGC TGAAAGAAAT GAGATGAACT TCCAAATGCG CGGTCGCTGG 
TACGAAAAAC TGAACGAATG GGAAAAAGCA CTCGGAGCAT ATGAACTTGA GGAAAAGAAA 
AAATCGTCAT GCCCCAATCT CCAGGTTTAT GATGAAAAAG ATCATTTAAT GACACCAGAA 
GAAGCAGCAA CAGCAGAAGA AGCTCGAATG CATGAGATGA GATGTTTAGA AGCATTGGGA 
AGATGGGATG AATTGAATAG TAAATCAGTA GTTTGGGCGG ATCAACGAGG AAATAGGAAT 
GATTCGGTTC GTGATGAGAT CAATAAGAAG CAATTGGATC ATAAAATGGC AGTGATTGCG 
GCAAGAGGAG CATGGGCTGT TGACAATTGG GAACGAATGG CTGATTATGT TTCAGTGATA 
TCTGAAAATA CACAAGACGG TGCAATGCTT CGAGCTGTGG TAGCTGTTCA CAATGATGAA 
AACACAAAAG CCATGGGGCT AATCGAAAAA GTTCGCGAAA TGATTGACAG TGAGCTGACA 
GCAATGGCTA ACGAAAGTTA CGAGAGAGCA TATATTCCAA TGGTTTCTGT TCAACAAATG 
GCTGAATTGG AAGAAGCTAT TGAATATAAG ACCCGTCCCG AACGTCGACC TCGAATCGCA 
CTTCTGTGGA GTCGACGCCT ACAAGGATGT CGGCGAAATG TGGAGCAATG GCAGAGACTT 
ATTATGCTCC GTGGACTTGT ATTATCTCCA CAAGAGATGC ATCCACTACG AGTCAAATTT 
TCATCAATGT GTCGGAAACA GGGAAAGAAT TCAATGTCTC GTGCAGTACT TCGAGAGCTT 
CTCTCTCTTC CAGCAAATTC TGATTTGGTG CGCGCAAAAG CTCCATTTGA CAAGCCGCTG 
TTGGTGTTGG CGTTGGCAAA ACAGTTGTAT CAGGATGATC ACAAAGATGA AGCAATTCGA 
GCACTTGAAG ATTTGGCGAA TCATTGGAAT AAAAGAATTA ATCCGATTCC AAAGGCGACT 
GGTAGAGAAC TGATTCCTCC GTCGACTAAA GAACCCGCGA GAATTTGTGC AAAAGTTTTG 
TTGAAACTTG GAGAATGGAC TGAGCTGAAA TCAAAGACTT CGAATAATAT GCAAGTCGGA 
GAATTATCAT TTGTTCGTCA ACAAGTATCA CCTCAATATC GAACAAAAGA ATCTCGTACA 
CCTGAGACAA TTGCCTTTGA GAATACCATA AATTACTATC AACAGGCAAC ACAATACGAT 
CCTGGATGGC ATAAAGTTTG GCACAAATTG GCTTCTACAC ATTTCTATGC TGTCTGTCGA 
GAACGACCAC ATCCAACAAC GGTCATTTCA CCGCCGCAGC AGCCTCAACA ACCGAAGAAA 
ATGCATATTC CACCAGTTAC GAGGGCAACA TCTCCACCTC CACCGGCTCA AAAGTCGCCT 
CAGCCTGCTC CATTTCATAG TATTACAGAG CCACTCTCTG ATTACCCTGT TCCACCACCA 
CTTGGTTCAC TCGTCGGTCT TCCACCAATG CCAGCTTATT TGTCTTCGAA TTCATCACTT 
CCACCACAAC ATCATCATGT CTCACCATTG AGCAACGATT CTCCATCGAA TTCAGCTGAA 
AATAAGCTTT ATTTGAAACA CGCGGCTCAC GCTGTGCGAT GCTTCGCAAA GGCTCTGATG 
TGCTCACCGG GTTCTCGACT TGAAGATACT CTCCGATTGA TGCAATTGTG GTTTGATCAT 
GGAGATGATA AAGATCAAGA TGTTTATTTT GCTCTTACGG AGACAATCTT TGATCTTCCT 
GTGACAACAT GGTTAGAAGC TATTCCTCAA TTGATGGCTC GTTTGGATTG TCCAGATGAT 
CAGAAAAGTG TTCAACTTGT TCTTCGAGTT CTTTGTGAAA TTGCCAGGCA TAGACCACAA 
GCAGTAATCT ATGCACTAAC TGTTGCATCG AGAAGTAAGG ATGTACATAG AAGTAAGAAT 
GCTGGAACAG TTCTCGAAAA AATGATGGAA TATCATTCAA AATTGGTTCG AGAGGCAAGT 
CTTGTGACAG AAGAGCTTGT TCGTTGCGCA ATTCTGTGGC ATGAACAGTG GCATGATGCA 
CTTGATGATG CGAGCCGTGT GTATTTCCAC GATAACAATG TGCAGGCGAT GTTTGATGCT 
TTGAGAAATA TGAATGATTT GATGCAAAAA GGAGCTCCAA CAACCATGAA AGAGCACAGT 
TTCCAACAGA CGTATAGTTC CGATTTGAAA GAAGCAGGAC GATATGTACA AGCATTTGAG 
AGTAGTGGAA ATGTGAAAGA TCTTAATCAG GCGTGGGAGA TTTATTGTTC GGTTTTCAAA 
AAGCTGCGTG ATCAACTTGC CACTTTGAAC TCGTTGGATC TTGTCTACGT ATCACCTAAT 
TTGGTCAGCG CAAAAGATTT GGAACTCGTT GTTCCTGGTA CATACGATCC ATCAGCTCCA 
ATAGTTTCCA TTCAAAGTTT CAGCTCAAAA ATGAATGTAA TCACTAGCAA GCAACGTCCT 
CGTAAAATGG TGATCCGTGG ATCAAATGGT TTAGACTATC AATTCTTGCT AAAAGGACAT 
GAAGATCCGA GACAAGATGA ACGTGTGATG CAACTTTTTG GACTAGTCAA CACACTTCTT 
GCCAACAATT CTGAGACTTG TCGCCGCAAT TTGACTATTC AAAGATACTC GATTGTAGCT 
TTGAGCAAAG ATTCTGGATT GATTGGTTGG GTTCCAAATT GTGATACACT TCATACACTT 
GTCAAGGAAT ATCGAGAAAA GAAAGCAAAG ATTCCATTGT CGATTGAGCA CAAAACACTT 
CAGAAATTAT CACTTGAAAC TGAACATTTG ACTTTAATGC AGAAGCTTCA ATTATTTGAA 
AGTGCTCTAA GTGTAACACA AGGAGAAGAT CTCCGACATG TATTATGGTT GAAGAGTCCG 
AGTTCTGAAG TTTGGTTTGA TCGACGAACC AATTATACAA GATCAGTTGC ATGTATGTCA 
ATGGTTGGAT ATATTCTTGG TCTCGGTGAC AGACATCCAT CCAACTTGAT GCTCGATCGA 
TTGACCGGAA AAGTTGTACA CATTGATTTC GGTGACTGTT TCGAAGTTGC TATGCTCCGT 
GAAAAATTCC CTGAACGAGT TCCATTCCGA CTTACCAGAA TGCTCATCAA CGCTATGGAA 
GTAACTGGAT TGGACGGAGT ATACAATTAC ACTGCCGAAC GTGTCTTGAA AATGTTGCGA 
ACAAATCAAG AATCATTGTT AGCAGTTTTA GAAGCATTTG TCTATGATCC AGTAATTAAT 
TGGCGTCTTG TGGAAGGAAT GAAAAAGGAT CCGAAAACTA GAAAAGATAC AGGTGGACGT 
CAGAATATGG CTGGTGCTGT ATTACCATCA TCAAGTACAA CTGATTCAAT TATGGAGACG 
ATTAAGAGGA AACTCGATGG GACCGAATTC GTTCACACTG ACGGATCAAC TCCACCTGAA 
CCACTTCAAG TAACTGAGCA ACTTGCAATG CTCACAGAGC AAGCAACATC TCCATTGAAT 
TTGTGTCAAA GTTATATTGG ATGGTGTCCG TTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-363 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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