gcy-28 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gcy-28
Entrez Gene ID	172051
Full Name	Receptor-type guanylate cyclase gcy-28
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001026429.2	NP_001021600.1	Receptor-type guanylate cyclase gcy-28
NM_001262699.2	NP_001249628.1	Receptor-type guanylate cyclase gcy-28
NM_058978.4	NP_491379.3	Receptor-type guanylate cyclase gcy-28
NM_001026429.3	NP_001021600.1	Receptor-type guanylate cyclase gcy-28
NM_001262699.1	NP_001249628.1	Receptor-type guanylate cyclase gcy-28
NM_058979.1	NP_491380.1	Receptor-type guanylate cyclase gcy-28
NM_001372632.1	NP_001360115.1	Receptor-type guanylate cyclase gcy-28
NM_058978.3	NP_491379.3	Receptor-type guanylate cyclase gcy-28

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Npr2	NP_446290.1
Mus musculus (house mouse)	Npr2	NP_776149.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003283	XP_307791.5
Gallus gallus (chicken)	NPR2	XP_004937202.1
Drosophila melanogaster (fruit fly)	CG31183	NP_650505.2
Homo sapiens (human)	NPR2	NP_003986.2
Pan troglodytes (chimpanzee)	NPR2	XP_001168194.1
	NPR2	XP_001085221.1
Canis lupus familiaris (dog)	NPR2	XP_005626847.1
Caenorhabditis elegans (roundworm)	gcy-28	NP_001249628.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GCY-28 and SCD-2 regulate sensory integration by modulating the activity of interneurons. Proper sensory integration is important for proper behavioral choices.
Title: Behavioral choice between conflicting alternatives is regulated by a receptor guanylyl cyclase, GCY-28, and a receptor tyrosine kinase, SCD-2, in AIA interneurons of Caenorhabditis elegans.

Mutations in the receptor-type guanylate cyclase gcy-28 lead to an avoidance behavior in place of the attractive behavior normally directed by the olfactory AWC(ON) neuron.
Title: A behavioral switch: cGMP and PKC signaling in olfactory neurons reverses odor preference in C. elegans.

The following gcy-28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcy-28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337135	NM_058979.1 Latest version!	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa337135	NM_001372632.1 Latest version!	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa337134	NM_058978.4 Latest version!	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa337136	NM_001026429.3 Latest version!	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa337137	NM_001262699.2 Latest version!	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa337136	NM_001026429.2	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa337137	NM_001262699.1	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa337134	NM_058978.3	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcy-28 ( NM_058979.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491380.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

Price & Availability↑

CloneID	OCa337135
Clone ID Related Accession (Same CDS sequence)	NM_058979.1 , NM_001372632.1
Accession Version	NM_058979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGATTGATC GAAAGTTGAG CATTTTCACG CGAAAAAAGA GCACACCACC ATCTGAATCA 
CAAAAGAACG GAGGACTCAC ACCGAATTCG CTTCAAAAAG CGGAAAATGG AGATTGTTCA 
CCGATTAATG AAGTTCAATT CAGACTTCCA TTAAATGATC GGAGAGTATC ATCACCATCA 
TCAGAAGCGA CGAGGAAAAA GAATAGTAAT GAAGAAGATC CGGAGAATGG TGCAAAGAAA 
AGTTTGAGCT TGAAAAATCG AAAACTCAGC TTTGGAATGG TTTCATTCAA GAGTGGAAGT 
GGTGGATCAG TGGAAACAAT TGCTCAAAAT AATACGCAAA TATACACGAA AACGGCAATA 
TTCAAGGGAG TTGTTGTGGC GATCAAGAAG TTGAATATAG ATCCAAAGAA GTATCCACGT 
CTTGATTTAT CACGTGCTCA ATTGATGGAA TTAAAAAAGA TGAAGGATCT TCAACATGAT 
CATATTACGA GATTCACCGG AGCATGTATC GATTTTCCAC ATTATTGTGT AGTAACTGAA 
TACTGTCCGA AAGGTTCATT GGAAGATATA TTGGAAAATG AAAAAATCGA ATTGGACAAA 
CTGATGAAGT ACTCACTACT TCACGATTTG GTTAAGGGTC TATTCTTCCT GCACAATAGT 
GAAATTCGAT CTCACGGACG TCTGAAATCA TCGAATTGTG TCGTTGACAG TCGATTTGTG 
CTGAAAGTCA CCGATTTCGG ACTTCACAGG CTTCATTGTT TAGAAGAAAT TAATTTGGAA 
GAAATTGGAG AGCACGCGTA TTATAAAAAA ATGCTGTGGA CGGCTCCAGA ATTGCTCCGA 
GATTCGAATG CTCCACCAAT GGGAACCCAG AAGGGTGATA TCTATTCATT TGCAATTATT 
TTGCATGAAA TGATGTTCCG GAAGGGAGTT TTCGCATTGG AGAATGAGGA TTTATCACCG 
AATGAAATAG TGCAACGGGT ACGAAAACCA GTATCAGAAG ATCAAGAACC ACTTCGACCG 
TGGGTATCAG AAACTGGTGA AGGTGAAGGT GATGATGCAT TAAATGATAC ATTATTATCA 
TTAATGGTTG CATGTTGGTC TGAAGATCCA CATGAACGGC CTGAAGTATC ATCTGTTCGA 
AAAGCGGTTC GAAGTTTGAA TCGAGATAAT GAAACATCGA ATCTTGTGGA TAATTTACTC 
AAAAGAATGG AACAATATGC TAATAATTTG GAAGGTCTTG TTGAGGAACG CACGCAAGAA 
TATTTGGCGG AAAAGAAAAA GGTAGAAGAA CTTCTTCATC AACTTCTTCC ACCTGCAATC 
GCCGACACTT TAATTGCTGG ACGAGCTGTT CAAGCTGAAT CGTATGATTG TGTAACTATC 
TACTTTTCTG ATATTGTCGG ATTCACATCA CTATCATCAC AATCAACACC AATGCAAGTG 
GTCACATTAC TCAATGATTT ATATTTGGCA TTTGATGGAG TTGTAGACAA TTTCAAAGTA 
TATAAAGTTG AGACTATTGG TGATGCATAC ATGGTAGTAT CTGGATTACC AGAACGACGA 
GATGATCATG CTAATCAAAT AGCTCAAATG TCACTATCAT TATTGCATAA AGTTAAGAAT 
TTTGTGATTC GACATCGACC TCATGAACAG CTCAAACTTC GTATCGGAAT GCATTCAGGA 
TCCGTTGTGG CAGGAGTCGT AGGTTCAAAA ATGCCTCGAT ACTGTCTTTT CGGAGATACT 
GTGAACACTT CGAGTCGAAT GGAAAGCAAT GGATTACGTA TGTTTTGGAA TTTTTCGTTA 
TATAAAATTT ACAAATTTAA GGTTTTTAAA AATAAAATTG AGTTGGATTT TCTGAAAAAC 
GAAAAAGAAT CAATTGAATT TGAAGGCAAA AAAATGGTTA CCGAATTTAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491380.1
CDS	1..1914
Translation	MIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLRMFWNFSLYKIYKFKVFKNKIELDFLKNEKESIEFEGKKMVTEFN

Target ORF information:

RefSeq Version	NM_058979.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058979.1

ORF Insert Sequence:

ATGATTGATC GAAAGTTGAG CATTTTCACG CGAAAAAAGA GCACACCACC ATCTGAATCA 
CAAAAGAACG GAGGACTCAC ACCGAATTCG CTTCAAAAAG CGGAAAATGG AGATTGTTCA 
CCGATTAATG AAGTTCAATT CAGACTTCCA TTAAATGATC GGAGAGTATC ATCACCATCA 
TCAGAAGCGA CGAGGAAAAA GAATAGTAAT GAAGAAGATC CGGAGAATGG TGCAAAGAAA 
AGTTTGAGCT TGAAAAATCG AAAACTCAGC TTTGGAATGG TTTCATTCAA GAGTGGAAGT 
GGTGGATCAG TGGAAACAAT TGCTCAAAAT AATACGCAAA TATACACGAA AACGGCAATA 
TTCAAGGGAG TTGTTGTGGC GATCAAGAAG TTGAATATAG ATCCAAAGAA GTATCCACGT 
CTTGATTTAT CACGTGCTCA ATTGATGGAA TTAAAAAAGA TGAAGGATCT TCAACATGAT 
CATATTACGA GATTCACCGG AGCATGTATC GATTTTCCAC ATTATTGTGT AGTAACTGAA 
TACTGTCCGA AAGGTTCATT GGAAGATATA TTGGAAAATG AAAAAATCGA ATTGGACAAA 
CTGATGAAGT ACTCACTACT TCACGATTTG GTTAAGGGTC TATTCTTCCT GCACAATAGT 
GAAATTCGAT CTCACGGACG TCTGAAATCA TCGAATTGTG TCGTTGACAG TCGATTTGTG 
CTGAAAGTCA CCGATTTCGG ACTTCACAGG CTTCATTGTT TAGAAGAAAT TAATTTGGAA 
GAAATTGGAG AGCACGCGTA TTATAAAAAA ATGCTGTGGA CGGCTCCAGA ATTGCTCCGA 
GATTCGAATG CTCCACCAAT GGGAACCCAG AAGGGTGATA TCTATTCATT TGCAATTATT 
TTGCATGAAA TGATGTTCCG GAAGGGAGTT TTCGCATTGG AGAATGAGGA TTTATCACCG 
AATGAAATAG TGCAACGGGT ACGAAAACCA GTATCAGAAG ATCAAGAACC ACTTCGACCG 
TGGGTATCAG AAACTGGTGA AGGTGAAGGT GATGATGCAT TAAATGATAC ATTATTATCA 
TTAATGGTTG CATGTTGGTC TGAAGATCCA CATGAACGGC CTGAAGTATC ATCTGTTCGA 
AAAGCGGTTC GAAGTTTGAA TCGAGATAAT GAAACATCGA ATCTTGTGGA TAATTTACTC 
AAAAGAATGG AACAATATGC TAATAATTTG GAAGGTCTTG TTGAGGAACG CACGCAAGAA 
TATTTGGCGG AAAAGAAAAA GGTAGAAGAA CTTCTTCATC AACTTCTTCC ACCTGCAATC 
GCCGACACTT TAATTGCTGG ACGAGCTGTT CAAGCTGAAT CGTATGATTG TGTAACTATC 
TACTTTTCTG ATATTGTCGG ATTCACATCA CTATCATCAC AATCAACACC AATGCAAGTG 
GTCACATTAC TCAATGATTT ATATTTGGCA TTTGATGGAG TTGTAGACAA TTTCAAAGTA 
TATAAAGTTG AGACTATTGG TGATGCATAC ATGGTAGTAT CTGGATTACC AGAACGACGA 
GATGATCATG CTAATCAAAT AGCTCAAATG TCACTATCAT TATTGCATAA AGTTAAGAAT 
TTTGTGATTC GACATCGACC TCATGAACAG CTCAAACTTC GTATCGGAAT GCATTCAGGA 
TCCGTTGTGG CAGGAGTCGT AGGTTCAAAA ATGCCTCGAT ACTGTCTTTT CGGAGATACT 
GTGAACACTT CGAGTCGAAT GGAAAGCAAT GGATTACGTA TGTTTTGGAA TTTTTCGTTA 
TATAAAATTT ACAAATTTAA GGTTTTTAAA AATAAAATTG AGTTGGATTT TCTGAAAAAC 
GAAAAAGAAT CAATTGAATT TGAAGGCAAA AAAATGGTTA CCGAATTTAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_001372632.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360115.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Price & Availability↑

CloneID	OCa337135
Clone ID Related Accession (Same CDS sequence)	NM_058979.1 , NM_001372632.1
Accession Version	NM_001372632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279).

ATGATTGATC GAAAGTTGAG CATTTTCACG CGAAAAAAGA GCACACCACC ATCTGAATCA 
CAAAAGAACG GAGGACTCAC ACCGAATTCG CTTCAAAAAG CGGAAAATGG AGATTGTTCA 
CCGATTAATG AAGTTCAATT CAGACTTCCA TTAAATGATC GGAGAGTATC ATCACCATCA 
TCAGAAGCGA CGAGGAAAAA GAATAGTAAT GAAGAAGATC CGGAGAATGG TGCAAAGAAA 
AGTTTGAGCT TGAAAAATCG AAAACTCAGC TTTGGAATGG TTTCATTCAA GAGTGGAAGT 
GGTGGATCAG TGGAAACAAT TGCTCAAAAT AATACGCAAA TATACACGAA AACGGCAATA 
TTCAAGGGAG TTGTTGTGGC GATCAAGAAG TTGAATATAG ATCCAAAGAA GTATCCACGT 
CTTGATTTAT CACGTGCTCA ATTGATGGAA TTAAAAAAGA TGAAGGATCT TCAACATGAT 
CATATTACGA GATTCACCGG AGCATGTATC GATTTTCCAC ATTATTGTGT AGTAACTGAA 
TACTGTCCGA AAGGTTCATT GGAAGATATA TTGGAAAATG AAAAAATCGA ATTGGACAAA 
CTGATGAAGT ACTCACTACT TCACGATTTG GTTAAGGGTC TATTCTTCCT GCACAATAGT 
GAAATTCGAT CTCACGGACG TCTGAAATCA TCGAATTGTG TCGTTGACAG TCGATTTGTG 
CTGAAAGTCA CCGATTTCGG ACTTCACAGG CTTCATTGTT TAGAAGAAAT TAATTTGGAA 
GAAATTGGAG AGCACGCGTA TTATAAAAAA ATGCTGTGGA CGGCTCCAGA ATTGCTCCGA 
GATTCGAATG CTCCACCAAT GGGAACCCAG AAGGGTGATA TCTATTCATT TGCAATTATT 
TTGCATGAAA TGATGTTCCG GAAGGGAGTT TTCGCATTGG AGAATGAGGA TTTATCACCG 
AATGAAATAG TGCAACGGGT ACGAAAACCA GTATCAGAAG ATCAAGAACC ACTTCGACCG 
TGGGTATCAG AAACTGGTGA AGGTGAAGGT GATGATGCAT TAAATGATAC ATTATTATCA 
TTAATGGTTG CATGTTGGTC TGAAGATCCA CATGAACGGC CTGAAGTATC ATCTGTTCGA 
AAAGCGGTTC GAAGTTTGAA TCGAGATAAT GAAACATCGA ATCTTGTGGA TAATTTACTC 
AAAAGAATGG AACAATATGC TAATAATTTG GAAGGTCTTG TTGAGGAACG CACGCAAGAA 
TATTTGGCGG AAAAGAAAAA GGTAGAAGAA CTTCTTCATC AACTTCTTCC ACCTGCAATC 
GCCGACACTT TAATTGCTGG ACGAGCTGTT CAAGCTGAAT CGTATGATTG TGTAACTATC 
TACTTTTCTG ATATTGTCGG ATTCACATCA CTATCATCAC AATCAACACC AATGCAAGTG 
GTCACATTAC TCAATGATTT ATATTTGGCA TTTGATGGAG TTGTAGACAA TTTCAAAGTA 
TATAAAGTTG AGACTATTGG TGATGCATAC ATGGTAGTAT CTGGATTACC AGAACGACGA 
GATGATCATG CTAATCAAAT AGCTCAAATG TCACTATCAT TATTGCATAA AGTTAAGAAT 
TTTGTGATTC GACATCGACC TCATGAACAG CTCAAACTTC GTATCGGAAT GCATTCAGGA 
TCCGTTGTGG CAGGAGTCGT AGGTTCAAAA ATGCCTCGAT ACTGTCTTTT CGGAGATACT 
GTGAACACTT CGAGTCGAAT GGAAAGCAAT GGATTACGTA TGTTTTGGAA TTTTTCGTTA 
TATAAAATTT ACAAATTTAA GGTTTTTAAA AATAAAATTG AGTTGGATTT TCTGAAAAAC 
GAAAAAGAAT CAATTGAATT TGAAGGCAAA AAAATGGTTA CCGAATTTAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360115.1
CDS	1800..3713
Translation	MIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLRMFWNFSLYKIYKFKVFKNKIELDFLKNEKESIEFEGKKMVTEFN

Target ORF information:

RefSeq Version	NM_001372632.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372632.1

ORF Insert Sequence:

ATGATTGATC GAAAGTTGAG CATTTTCACG CGAAAAAAGA GCACACCACC ATCTGAATCA 
CAAAAGAACG GAGGACTCAC ACCGAATTCG CTTCAAAAAG CGGAAAATGG AGATTGTTCA 
CCGATTAATG AAGTTCAATT CAGACTTCCA TTAAATGATC GGAGAGTATC ATCACCATCA 
TCAGAAGCGA CGAGGAAAAA GAATAGTAAT GAAGAAGATC CGGAGAATGG TGCAAAGAAA 
AGTTTGAGCT TGAAAAATCG AAAACTCAGC TTTGGAATGG TTTCATTCAA GAGTGGAAGT 
GGTGGATCAG TGGAAACAAT TGCTCAAAAT AATACGCAAA TATACACGAA AACGGCAATA 
TTCAAGGGAG TTGTTGTGGC GATCAAGAAG TTGAATATAG ATCCAAAGAA GTATCCACGT 
CTTGATTTAT CACGTGCTCA ATTGATGGAA TTAAAAAAGA TGAAGGATCT TCAACATGAT 
CATATTACGA GATTCACCGG AGCATGTATC GATTTTCCAC ATTATTGTGT AGTAACTGAA 
TACTGTCCGA AAGGTTCATT GGAAGATATA TTGGAAAATG AAAAAATCGA ATTGGACAAA 
CTGATGAAGT ACTCACTACT TCACGATTTG GTTAAGGGTC TATTCTTCCT GCACAATAGT 
GAAATTCGAT CTCACGGACG TCTGAAATCA TCGAATTGTG TCGTTGACAG TCGATTTGTG 
CTGAAAGTCA CCGATTTCGG ACTTCACAGG CTTCATTGTT TAGAAGAAAT TAATTTGGAA 
GAAATTGGAG AGCACGCGTA TTATAAAAAA ATGCTGTGGA CGGCTCCAGA ATTGCTCCGA 
GATTCGAATG CTCCACCAAT GGGAACCCAG AAGGGTGATA TCTATTCATT TGCAATTATT 
TTGCATGAAA TGATGTTCCG GAAGGGAGTT TTCGCATTGG AGAATGAGGA TTTATCACCG 
AATGAAATAG TGCAACGGGT ACGAAAACCA GTATCAGAAG ATCAAGAACC ACTTCGACCG 
TGGGTATCAG AAACTGGTGA AGGTGAAGGT GATGATGCAT TAAATGATAC ATTATTATCA 
TTAATGGTTG CATGTTGGTC TGAAGATCCA CATGAACGGC CTGAAGTATC ATCTGTTCGA 
AAAGCGGTTC GAAGTTTGAA TCGAGATAAT GAAACATCGA ATCTTGTGGA TAATTTACTC 
AAAAGAATGG AACAATATGC TAATAATTTG GAAGGTCTTG TTGAGGAACG CACGCAAGAA 
TATTTGGCGG AAAAGAAAAA GGTAGAAGAA CTTCTTCATC AACTTCTTCC ACCTGCAATC 
GCCGACACTT TAATTGCTGG ACGAGCTGTT CAAGCTGAAT CGTATGATTG TGTAACTATC 
TACTTTTCTG ATATTGTCGG ATTCACATCA CTATCATCAC AATCAACACC AATGCAAGTG 
GTCACATTAC TCAATGATTT ATATTTGGCA TTTGATGGAG TTGTAGACAA TTTCAAAGTA 
TATAAAGTTG AGACTATTGG TGATGCATAC ATGGTAGTAT CTGGATTACC AGAACGACGA 
GATGATCATG CTAATCAAAT AGCTCAAATG TCACTATCAT TATTGCATAA AGTTAAGAAT 
TTTGTGATTC GACATCGACC TCATGAACAG CTCAAACTTC GTATCGGAAT GCATTCAGGA 
TCCGTTGTGG CAGGAGTCGT AGGTTCAAAA ATGCCTCGAT ACTGTCTTTT CGGAGATACT 
GTGAACACTT CGAGTCGAAT GGAAAGCAAT GGATTACGTA TGTTTTGGAA TTTTTCGTTA 
TATAAAATTT ACAAATTTAA GGTTTTTAAA AATAAAATTG AGTTGGATTT TCTGAAAAAC 
GAAAAAGAAT CAATTGAATT TGAAGGCAAA AAAATGGTTA CCGAATTTAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_058978.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491379.3 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Price & Availability↑

CloneID	OCa337134
Clone ID Related Accession (Same CDS sequence)	NM_058978.4 , NM_058978.3
Accession Version	NM_058978.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058978.3.

ATGATTCTAA GCGACGTACT ATTATTATCT CTGGTGATCA GCAGCAGCAG CAGTAGCAGT 
CATCAACATT CACTACTTCC TATTGATATT GTCGTCGGTC TGCCGATCGA AGAAGGCGAC 
CGTGGGAAGA ACCCATTCTT ATTAACTCTT GCCAAATCGA AGCCGGTGTT TGATGTGGCT 
CTTCAAGACG TGTATCACCT CCGTATCCTC CCATATGGAT CACTCAAAGT CACATTCGAG 
AACTCGGCTC TGTCGGATGC AGTCGGTCCG CAAAAAATGA TCGAGCACTA TTGCAACAAG 
ACGGTTGATG CGATAATGGG TCTTCCCTAC GTGTATGCTC TGGCTCCAGT TGCTCGTATT 
TCAAAGTTTT GGGGTCAAGG AGTTCCGGTG TTCACGACGA CTGCACTCGT TGATGAATTA 
GGCGATCGAA ACGAGTTTCC ATTGTTGACA AGAATGATGG GAAGTTACAA ATCACTTGGC 
AAACTGGTGA CTCGAATTGC TGAACGGTTC GAGTGGCAAC ATTACTTTTT CATGTTCAAT 
GATGAAGTTG CCCGAGGGCC TAGAAATAAA GGACGATCTG AATGTTACTT CTCACTGTCA 
GCTATAAAAA ATCTTATAAT GAACAATAAG ACTTCCACAT GGAATGTGAA AATGTTCAGC 
GAGTTTGAAG CCGATCGTCT ACAGTACCGA GCTCTGTTGA GTGAGGCATC AGTAATGAGC 
AACTTGATTA TCCTATGTGC ATCACCGGAT ACTGTACGTG AAATAATGTT GGCTGCTCAT 
GATCTTGGAA TGGCGACAAG TGGTGAATAT GTATTCATTA ATATCGATGT TTCAACGGGA 
TCACACGCCG AACAACCATG GATTCGAGCA AATGACACGA ATAATGAGGA GAACGAGAAA 
GCAAAAGAAG CATATCGAGC ATTGAAAACA ATTTCATTGA GACGATCCGA TTTGGATGAA 
TATAAGAATT TTGAACTTCG AGTCAAAGAA CGAGCAGATC AAAAATATAA TTATACGAAT 
ATCACCGGAA AAGATTATGA GATGAACAAT TTCATCAGTG CATTTTACGA TGCGGTTCTT 
CTCTATGCCA TTGCTCTAAA TGAGACAATT CAATCAGGTC TTGATCCACG AAATGGACAT 
AATATCACGT CACGAATGTG GGGACGAACA TTTGTCGGAA TTACTGGAAA TGTATCGATT 
GATCACAATG GTGATAGGTA TTCGGATTAT TCTCTACTCG ATTTGGATCC CGTTCAGAAT 
AGATTTGTGG AAGTAGCCTA CTACAGTGGA GCATCAAATC AACTAAAAAC TGTGGGACAA 
CTGCATTGGG TTGGCGGAAA ACCTCCAACA GATCTACCTA TATGTGGTTA CGATAAGAGC 
AAGTGTCCGC AGGGTTATCC ACTTCATGTC TATCTTCTAA TGGGTTCATT CCTCCTAATT 
CTCGTCCTTG TCGGTCTATT CATCTTCTTC TGGCGTCGCT ACAAACTTGA ACAAGAGCTC 
GCCGCGATGA GCTGGAAGAT TCGATGGGAA GAGCTGGATG GTGAAGAGAG TCAAAAGAAG 
AATGAAAAGA AGAAGGCGAA GAAAAGGAAA AATCATAACG ATTATCTACC TGAATCTGAT 
CCATTATTAA GATCCACATC GAGGTCGTCA GTGAATTCGG ACAAGAGATC GTCCAGCTCG 
GGAACAACTC GTAAAATCTC AGCGATGATT GATCGAAAGT TGAGCATTTT CACGCGAAAA 
AAGAGCACAC CACCATCTGA ATCACAAAAG AACGGAGGAC TCACACCGAA TTCGCTTCAA 
AAAGCGGAAA ATGGAGATTG TTCACCGATT AATGAAGTTC AATTCAGACT TCCATTAAAT 
GATCGGAGAG TATCATCACC ATCATCAGAA GCGACGAGGA AAAAGAATAG TAATGAAGAA 
GATCCGGAGA ATGGTGCAAA GAAAAGTTTG AGCTTGAAAA ATCGAAAACT CAGCTTTGGA 
ATGGTTTCAT TCAAGAGTGG AAGTGGTGGA TCAGTGGAAA CAATTGCTCA AAATAATACG 
CAAATATACA CGAAAACGGC AATATTCAAG GGAGTTGTTG TGGCGATCAA GAAGTTGAAT 
ATAGATCCAA AGAAGTATCC ACGTCTTGAT TTATCACGTG CTCAATTGAT GGAATTAAAA 
AAGATGAAGG ATCTTCAACA TGATCATATT ACGAGATTCA CCGGAGCATG TATCGATTTT 
CCACATTATT GTGTAGTAAC TGAATACTGT CCGAAAGGTT CATTGGAAGA TATATTGGAA 
AATGAAAAAA TCGAATTGGA CAAACTGATG AAGTACTCAC TACTTCACGA TTTGGTTAAG 
GGTCTATTCT TCCTGCACAA TAGTGAAATT CGATCTCACG GACGTCTGAA ATCATCGAAT 
TGTGTCGTTG ACAGTCGATT TGTGCTGAAA GTCACCGATT TCGGACTTCA CAGGCTTCAT 
TGTTTAGAAG AAATTAATTT GGAAGAAATT GGAGAGCACG CGTATTATAA AAAAATGCTG 
TGGACGGCTC CAGAATTGCT CCGAGATTCG AATGCTCCAC CAATGGGAAC CCAGAAGGGT 
GATATCTATT CATTTGCAAT TATTTTGCAT GAAATGATGT TCCGGAAGGG AGTTTTCGCA 
TTGGAGAATG AGGATTTATC ACCGAATGAA ATAGTGCAAC GGGTACGAAA ACCAGTATCA 
GAAGATCAAG AACCACTTCG ACCGTGGGTA TCAGAAACTG GTGAAGGTGA AGGTGATGAT 
GCATTAAATG ATACATTATT ATCATTAATG GTTGCATGTT GGTCTGAAGA TCCACATGAA 
CGGCCTGAAG TATCATCTGT TCGAAAAGCG GTTCGAAGTT TGAATCGAGA TAATGAAACA 
TCGAATCTTG TGGATAATTT ACTCAAAAGA ATGGAACAAT ATGCTAATAA TTTGGAAGGT 
CTTGTTGAGG AACGCACGCA AGAATATTTG GCGGAAAAGA AAAAGGTAGA AGAACTTCTT 
CATCAACTTC TTCCACCTGC AATCGCCGAC ACTTTAATTG CTGGACGAGC TGTTCAAGCT 
GAATCGTATG ATTGTGTAAC TATCTACTTT TCTGATATTG TCGGATTCAC ATCACTATCA 
TCACAATCAA CACCAATGCA AGTGGTCACA TTACTCAATG ATTTATATTT GGCATTTGAT 
GGAGTTGTAG ACAATTTCAA AGTATATAAA GTTGAGACTA TTGGTGATGC ATACATGGTA 
GTATCTGGAT TACCAGAACG ACGAGATGAT CATGCTAATC AAATAGCTCA AATGTCACTA 
TCATTATTGC ATAAAGTTAA GAATTTTGTG ATTCGACATC GACCTCATGA ACAGCTCAAA 
CTTCGTATCG GAATGCATTC AGGATCCGTT GTGGCAGGAG TCGTAGGTTC AAAAATGCCT 
CGATACTGTC TTTTCGGAGA TACTGTGAAC ACTTCGAGTC GAATGGAAAG CAATGGATTA 
CCTCTCAAAA TCCACGTGTC ACAACAAACG TACGATATTC TGATGCAAGA AGCGGGCTTT 
AAACTCGAAC TACGAGGTTC AGTTGAGATG AAAGGCAAAG GAATGCAGAC TACTTATTGG 
TTACGAGGTT ACAAAGACGT TGAGATTCCC GATTTTGGCG AAGAATTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491379.3
CDS	195..3848
Translation	MILSDVLLLSLVISSSSSSSHQHSLLPIDIVVGLPIEEGDRGKNPFLLTLAKSKPVFDVALQDVYHLRILPYGSLKVTFENSALSDAVGPQKMIEHYCNKTVDAIMGLPYVYALAPVARISKFWGQGVPVFTTTALVDELGDRNEFPLLTRMMGSYKSLGKLVTRIAERFEWQHYFFMFNDEVARGPRNKGRSECYFSLSAIKNLIMNNKTSTWNVKMFSEFEADRLQYRALLSEASVMSNLIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPQGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_058978.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058978.4

ORF Insert Sequence:

ATGATTCTAA GCGACGTACT ATTATTATCT CTGGTGATCA GCAGCAGCAG CAGTAGCAGT 
CATCAACATT CACTACTTCC TATTGATATT GTCGTCGGTC TGCCGATCGA AGAAGGCGAC 
CGTGGGAAGA ACCCATTCTT ATTAACTCTT GCCAAATCGA AGCCGGTGTT TGATGTGGCT 
CTTCAAGACG TGTATCACCT CCGTATCCTC CCATATGGAT CACTCAAAGT CACATTCGAG 
AACTCGGCTC TGTCGGATGC AGTCGGTCCG CAAAAAATGA TCGAGCACTA TTGCAACAAG 
ACGGTTGATG CGATAATGGG TCTTCCCTAC GTGTATGCTC TGGCTCCAGT TGCTCGTATT 
TCAAAGTTTT GGGGTCAAGG AGTTCCGGTG TTCACGACGA CTGCACTCGT TGATGAATTA 
GGCGATCGAA ACGAGTTTCC ATTGTTGACA AGAATGATGG GAAGTTACAA ATCACTTGGC 
AAACTGGTGA CTCGAATTGC TGAACGGTTC GAGTGGCAAC ATTACTTTTT CATGTTCAAT 
GATGAAGTTG CCCGAGGGCC TAGAAATAAA GGACGATCTG AATGTTACTT CTCACTGTCA 
GCTATAAAAA ATCTTATAAT GAACAATAAG ACTTCCACAT GGAATGTGAA AATGTTCAGC 
GAGTTTGAAG CCGATCGTCT ACAGTACCGA GCTCTGTTGA GTGAGGCATC AGTAATGAGC 
AACTTGATTA TCCTATGTGC ATCACCGGAT ACTGTACGTG AAATAATGTT GGCTGCTCAT 
GATCTTGGAA TGGCGACAAG TGGTGAATAT GTATTCATTA ATATCGATGT TTCAACGGGA 
TCACACGCCG AACAACCATG GATTCGAGCA AATGACACGA ATAATGAGGA GAACGAGAAA 
GCAAAAGAAG CATATCGAGC ATTGAAAACA ATTTCATTGA GACGATCCGA TTTGGATGAA 
TATAAGAATT TTGAACTTCG AGTCAAAGAA CGAGCAGATC AAAAATATAA TTATACGAAT 
ATCACCGGAA AAGATTATGA GATGAACAAT TTCATCAGTG CATTTTACGA TGCGGTTCTT 
CTCTATGCCA TTGCTCTAAA TGAGACAATT CAATCAGGTC TTGATCCACG AAATGGACAT 
AATATCACGT CACGAATGTG GGGACGAACA TTTGTCGGAA TTACTGGAAA TGTATCGATT 
GATCACAATG GTGATAGGTA TTCGGATTAT TCTCTACTCG ATTTGGATCC CGTTCAGAAT 
AGATTTGTGG AAGTAGCCTA CTACAGTGGA GCATCAAATC AACTAAAAAC TGTGGGACAA 
CTGCATTGGG TTGGCGGAAA ACCTCCAACA GATCTACCTA TATGTGGTTA CGATAAGAGC 
AAGTGTCCGC AGGGTTATCC ACTTCATGTC TATCTTCTAA TGGGTTCATT CCTCCTAATT 
CTCGTCCTTG TCGGTCTATT CATCTTCTTC TGGCGTCGCT ACAAACTTGA ACAAGAGCTC 
GCCGCGATGA GCTGGAAGAT TCGATGGGAA GAGCTGGATG GTGAAGAGAG TCAAAAGAAG 
AATGAAAAGA AGAAGGCGAA GAAAAGGAAA AATCATAACG ATTATCTACC TGAATCTGAT 
CCATTATTAA GATCCACATC GAGGTCGTCA GTGAATTCGG ACAAGAGATC GTCCAGCTCG 
GGAACAACTC GTAAAATCTC AGCGATGATT GATCGAAAGT TGAGCATTTT CACGCGAAAA 
AAGAGCACAC CACCATCTGA ATCACAAAAG AACGGAGGAC TCACACCGAA TTCGCTTCAA 
AAAGCGGAAA ATGGAGATTG TTCACCGATT AATGAAGTTC AATTCAGACT TCCATTAAAT 
GATCGGAGAG TATCATCACC ATCATCAGAA GCGACGAGGA AAAAGAATAG TAATGAAGAA 
GATCCGGAGA ATGGTGCAAA GAAAAGTTTG AGCTTGAAAA ATCGAAAACT CAGCTTTGGA 
ATGGTTTCAT TCAAGAGTGG AAGTGGTGGA TCAGTGGAAA CAATTGCTCA AAATAATACG 
CAAATATACA CGAAAACGGC AATATTCAAG GGAGTTGTTG TGGCGATCAA GAAGTTGAAT 
ATAGATCCAA AGAAGTATCC ACGTCTTGAT TTATCACGTG CTCAATTGAT GGAATTAAAA 
AAGATGAAGG ATCTTCAACA TGATCATATT ACGAGATTCA CCGGAGCATG TATCGATTTT 
CCACATTATT GTGTAGTAAC TGAATACTGT CCGAAAGGTT CATTGGAAGA TATATTGGAA 
AATGAAAAAA TCGAATTGGA CAAACTGATG AAGTACTCAC TACTTCACGA TTTGGTTAAG 
GGTCTATTCT TCCTGCACAA TAGTGAAATT CGATCTCACG GACGTCTGAA ATCATCGAAT 
TGTGTCGTTG ACAGTCGATT TGTGCTGAAA GTCACCGATT TCGGACTTCA CAGGCTTCAT 
TGTTTAGAAG AAATTAATTT GGAAGAAATT GGAGAGCACG CGTATTATAA AAAAATGCTG 
TGGACGGCTC CAGAATTGCT CCGAGATTCG AATGCTCCAC CAATGGGAAC CCAGAAGGGT 
GATATCTATT CATTTGCAAT TATTTTGCAT GAAATGATGT TCCGGAAGGG AGTTTTCGCA 
TTGGAGAATG AGGATTTATC ACCGAATGAA ATAGTGCAAC GGGTACGAAA ACCAGTATCA 
GAAGATCAAG AACCACTTCG ACCGTGGGTA TCAGAAACTG GTGAAGGTGA AGGTGATGAT 
GCATTAAATG ATACATTATT ATCATTAATG GTTGCATGTT GGTCTGAAGA TCCACATGAA 
CGGCCTGAAG TATCATCTGT TCGAAAAGCG GTTCGAAGTT TGAATCGAGA TAATGAAACA 
TCGAATCTTG TGGATAATTT ACTCAAAAGA ATGGAACAAT ATGCTAATAA TTTGGAAGGT 
CTTGTTGAGG AACGCACGCA AGAATATTTG GCGGAAAAGA AAAAGGTAGA AGAACTTCTT 
CATCAACTTC TTCCACCTGC AATCGCCGAC ACTTTAATTG CTGGACGAGC TGTTCAAGCT 
GAATCGTATG ATTGTGTAAC TATCTACTTT TCTGATATTG TCGGATTCAC ATCACTATCA 
TCACAATCAA CACCAATGCA AGTGGTCACA TTACTCAATG ATTTATATTT GGCATTTGAT 
GGAGTTGTAG ACAATTTCAA AGTATATAAA GTTGAGACTA TTGGTGATGC ATACATGGTA 
GTATCTGGAT TACCAGAACG ACGAGATGAT CATGCTAATC AAATAGCTCA AATGTCACTA 
TCATTATTGC ATAAAGTTAA GAATTTTGTG ATTCGACATC GACCTCATGA ACAGCTCAAA 
CTTCGTATCG GAATGCATTC AGGATCCGTT GTGGCAGGAG TCGTAGGTTC AAAAATGCCT 
CGATACTGTC TTTTCGGAGA TACTGTGAAC ACTTCGAGTC GAATGGAAAG CAATGGATTA 
CCTCTCAAAA TCCACGTGTC ACAACAAACG TACGATATTC TGATGCAAGA AGCGGGCTTT 
AAACTCGAAC TACGAGGTTC AGTTGAGATG AAAGGCAAAG GAATGCAGAC TACTTATTGG 
TTACGAGGTT ACAAAGACGT TGAGATTCCC GATTTTGGCG AAGAATTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_058978.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491379.3 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

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CloneID	OCa337134
Clone ID Related Accession (Same CDS sequence)	NM_058978.4 , NM_058978.3
Accession Version	NM_058978.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Aug 9, 2005 this sequence version replaced NM_058978.2. COMPLETENESS: incomplete on both ends.

ATGATTCTAA GCGACGTACT ATTATTATCT CTGGTGATCA GCAGCAGCAG CAGTAGCAGT 
CATCAACATT CACTACTTCC TATTGATATT GTCGTCGGTC TGCCGATCGA AGAAGGCGAC 
CGTGGGAAGA ACCCATTCTT ATTAACTCTT GCCAAATCGA AGCCGGTGTT TGATGTGGCT 
CTTCAAGACG TGTATCACCT CCGTATCCTC CCATATGGAT CACTCAAAGT CACATTCGAG 
AACTCGGCTC TGTCGGATGC AGTCGGTCCG CAAAAAATGA TCGAGCACTA TTGCAACAAG 
ACGGTTGATG CGATAATGGG TCTTCCCTAC GTGTATGCTC TGGCTCCAGT TGCTCGTATT 
TCAAAGTTTT GGGGTCAAGG AGTTCCGGTG TTCACGACGA CTGCACTCGT TGATGAATTA 
GGCGATCGAA ACGAGTTTCC ATTGTTGACA AGAATGATGG GAAGTTACAA ATCACTTGGC 
AAACTGGTGA CTCGAATTGC TGAACGGTTC GAGTGGCAAC ATTACTTTTT CATGTTCAAT 
GATGAAGTTG CCCGAGGGCC TAGAAATAAA GGACGATCTG AATGTTACTT CTCACTGTCA 
GCTATAAAAA ATCTTATAAT GAACAATAAG ACTTCCACAT GGAATGTGAA AATGTTCAGC 
GAGTTTGAAG CCGATCGTCT ACAGTACCGA GCTCTGTTGA GTGAGGCATC AGTAATGAGC 
AACTTGATTA TCCTATGTGC ATCACCGGAT ACTGTACGTG AAATAATGTT GGCTGCTCAT 
GATCTTGGAA TGGCGACAAG TGGTGAATAT GTATTCATTA ATATCGATGT TTCAACGGGA 
TCACACGCCG AACAACCATG GATTCGAGCA AATGACACGA ATAATGAGGA GAACGAGAAA 
GCAAAAGAAG CATATCGAGC ATTGAAAACA ATTTCATTGA GACGATCCGA TTTGGATGAA 
TATAAGAATT TTGAACTTCG AGTCAAAGAA CGAGCAGATC AAAAATATAA TTATACGAAT 
ATCACCGGAA AAGATTATGA GATGAACAAT TTCATCAGTG CATTTTACGA TGCGGTTCTT 
CTCTATGCCA TTGCTCTAAA TGAGACAATT CAATCAGGTC TTGATCCACG AAATGGACAT 
AATATCACGT CACGAATGTG GGGACGAACA TTTGTCGGAA TTACTGGAAA TGTATCGATT 
GATCACAATG GTGATAGGTA TTCGGATTAT TCTCTACTCG ATTTGGATCC CGTTCAGAAT 
AGATTTGTGG AAGTAGCCTA CTACAGTGGA GCATCAAATC AACTAAAAAC TGTGGGACAA 
CTGCATTGGG TTGGCGGAAA ACCTCCAACA GATCTACCTA TATGTGGTTA CGATAAGAGC 
AAGTGTCCGC AGGGTTATCC ACTTCATGTC TATCTTCTAA TGGGTTCATT CCTCCTAATT 
CTCGTCCTTG TCGGTCTATT CATCTTCTTC TGGCGTCGCT ACAAACTTGA ACAAGAGCTC 
GCCGCGATGA GCTGGAAGAT TCGATGGGAA GAGCTGGATG GTGAAGAGAG TCAAAAGAAG 
AATGAAAAGA AGAAGGCGAA GAAAAGGAAA AATCATAACG ATTATCTACC TGAATCTGAT 
CCATTATTAA GATCCACATC GAGGTCGTCA GTGAATTCGG ACAAGAGATC GTCCAGCTCG 
GGAACAACTC GTAAAATCTC AGCGATGATT GATCGAAAGT TGAGCATTTT CACGCGAAAA 
AAGAGCACAC CACCATCTGA ATCACAAAAG AACGGAGGAC TCACACCGAA TTCGCTTCAA 
AAAGCGGAAA ATGGAGATTG TTCACCGATT AATGAAGTTC AATTCAGACT TCCATTAAAT 
GATCGGAGAG TATCATCACC ATCATCAGAA GCGACGAGGA AAAAGAATAG TAATGAAGAA 
GATCCGGAGA ATGGTGCAAA GAAAAGTTTG AGCTTGAAAA ATCGAAAACT CAGCTTTGGA 
ATGGTTTCAT TCAAGAGTGG AAGTGGTGGA TCAGTGGAAA CAATTGCTCA AAATAATACG 
CAAATATACA CGAAAACGGC AATATTCAAG GGAGTTGTTG TGGCGATCAA GAAGTTGAAT 
ATAGATCCAA AGAAGTATCC ACGTCTTGAT TTATCACGTG CTCAATTGAT GGAATTAAAA 
AAGATGAAGG ATCTTCAACA TGATCATATT ACGAGATTCA CCGGAGCATG TATCGATTTT 
CCACATTATT GTGTAGTAAC TGAATACTGT CCGAAAGGTT CATTGGAAGA TATATTGGAA 
AATGAAAAAA TCGAATTGGA CAAACTGATG AAGTACTCAC TACTTCACGA TTTGGTTAAG 
GGTCTATTCT TCCTGCACAA TAGTGAAATT CGATCTCACG GACGTCTGAA ATCATCGAAT 
TGTGTCGTTG ACAGTCGATT TGTGCTGAAA GTCACCGATT TCGGACTTCA CAGGCTTCAT 
TGTTTAGAAG AAATTAATTT GGAAGAAATT GGAGAGCACG CGTATTATAA AAAAATGCTG 
TGGACGGCTC CAGAATTGCT CCGAGATTCG AATGCTCCAC CAATGGGAAC CCAGAAGGGT 
GATATCTATT CATTTGCAAT TATTTTGCAT GAAATGATGT TCCGGAAGGG AGTTTTCGCA 
TTGGAGAATG AGGATTTATC ACCGAATGAA ATAGTGCAAC GGGTACGAAA ACCAGTATCA 
GAAGATCAAG AACCACTTCG ACCGTGGGTA TCAGAAACTG GTGAAGGTGA AGGTGATGAT 
GCATTAAATG ATACATTATT ATCATTAATG GTTGCATGTT GGTCTGAAGA TCCACATGAA 
CGGCCTGAAG TATCATCTGT TCGAAAAGCG GTTCGAAGTT TGAATCGAGA TAATGAAACA 
TCGAATCTTG TGGATAATTT ACTCAAAAGA ATGGAACAAT ATGCTAATAA TTTGGAAGGT 
CTTGTTGAGG AACGCACGCA AGAATATTTG GCGGAAAAGA AAAAGGTAGA AGAACTTCTT 
CATCAACTTC TTCCACCTGC AATCGCCGAC ACTTTAATTG CTGGACGAGC TGTTCAAGCT 
GAATCGTATG ATTGTGTAAC TATCTACTTT TCTGATATTG TCGGATTCAC ATCACTATCA 
TCACAATCAA CACCAATGCA AGTGGTCACA TTACTCAATG ATTTATATTT GGCATTTGAT 
GGAGTTGTAG ACAATTTCAA AGTATATAAA GTTGAGACTA TTGGTGATGC ATACATGGTA 
GTATCTGGAT TACCAGAACG ACGAGATGAT CATGCTAATC AAATAGCTCA AATGTCACTA 
TCATTATTGC ATAAAGTTAA GAATTTTGTG ATTCGACATC GACCTCATGA ACAGCTCAAA 
CTTCGTATCG GAATGCATTC AGGATCCGTT GTGGCAGGAG TCGTAGGTTC AAAAATGCCT 
CGATACTGTC TTTTCGGAGA TACTGTGAAC ACTTCGAGTC GAATGGAAAG CAATGGATTA 
CCTCTCAAAA TCCACGTGTC ACAACAAACG TACGATATTC TGATGCAAGA AGCGGGCTTT 
AAACTCGAAC TACGAGGTTC AGTTGAGATG AAAGGCAAAG GAATGCAGAC TACTTATTGG 
TTACGAGGTT ACAAAGACGT TGAGATTCCC GATTTTGGCG AAGAATTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491379.3
CDS	1..3654
Translation	MILSDVLLLSLVISSSSSSSHQHSLLPIDIVVGLPIEEGDRGKNPFLLTLAKSKPVFDVALQDVYHLRILPYGSLKVTFENSALSDAVGPQKMIEHYCNKTVDAIMGLPYVYALAPVARISKFWGQGVPVFTTTALVDELGDRNEFPLLTRMMGSYKSLGKLVTRIAERFEWQHYFFMFNDEVARGPRNKGRSECYFSLSAIKNLIMNNKTSTWNVKMFSEFEADRLQYRALLSEASVMSNLIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPQGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_058978.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058978.3

ORF Insert Sequence:

ATGATTCTAA GCGACGTACT ATTATTATCT CTGGTGATCA GCAGCAGCAG CAGTAGCAGT 
CATCAACATT CACTACTTCC TATTGATATT GTCGTCGGTC TGCCGATCGA AGAAGGCGAC 
CGTGGGAAGA ACCCATTCTT ATTAACTCTT GCCAAATCGA AGCCGGTGTT TGATGTGGCT 
CTTCAAGACG TGTATCACCT CCGTATCCTC CCATATGGAT CACTCAAAGT CACATTCGAG 
AACTCGGCTC TGTCGGATGC AGTCGGTCCG CAAAAAATGA TCGAGCACTA TTGCAACAAG 
ACGGTTGATG CGATAATGGG TCTTCCCTAC GTGTATGCTC TGGCTCCAGT TGCTCGTATT 
TCAAAGTTTT GGGGTCAAGG AGTTCCGGTG TTCACGACGA CTGCACTCGT TGATGAATTA 
GGCGATCGAA ACGAGTTTCC ATTGTTGACA AGAATGATGG GAAGTTACAA ATCACTTGGC 
AAACTGGTGA CTCGAATTGC TGAACGGTTC GAGTGGCAAC ATTACTTTTT CATGTTCAAT 
GATGAAGTTG CCCGAGGGCC TAGAAATAAA GGACGATCTG AATGTTACTT CTCACTGTCA 
GCTATAAAAA ATCTTATAAT GAACAATAAG ACTTCCACAT GGAATGTGAA AATGTTCAGC 
GAGTTTGAAG CCGATCGTCT ACAGTACCGA GCTCTGTTGA GTGAGGCATC AGTAATGAGC 
AACTTGATTA TCCTATGTGC ATCACCGGAT ACTGTACGTG AAATAATGTT GGCTGCTCAT 
GATCTTGGAA TGGCGACAAG TGGTGAATAT GTATTCATTA ATATCGATGT TTCAACGGGA 
TCACACGCCG AACAACCATG GATTCGAGCA AATGACACGA ATAATGAGGA GAACGAGAAA 
GCAAAAGAAG CATATCGAGC ATTGAAAACA ATTTCATTGA GACGATCCGA TTTGGATGAA 
TATAAGAATT TTGAACTTCG AGTCAAAGAA CGAGCAGATC AAAAATATAA TTATACGAAT 
ATCACCGGAA AAGATTATGA GATGAACAAT TTCATCAGTG CATTTTACGA TGCGGTTCTT 
CTCTATGCCA TTGCTCTAAA TGAGACAATT CAATCAGGTC TTGATCCACG AAATGGACAT 
AATATCACGT CACGAATGTG GGGACGAACA TTTGTCGGAA TTACTGGAAA TGTATCGATT 
GATCACAATG GTGATAGGTA TTCGGATTAT TCTCTACTCG ATTTGGATCC CGTTCAGAAT 
AGATTTGTGG AAGTAGCCTA CTACAGTGGA GCATCAAATC AACTAAAAAC TGTGGGACAA 
CTGCATTGGG TTGGCGGAAA ACCTCCAACA GATCTACCTA TATGTGGTTA CGATAAGAGC 
AAGTGTCCGC AGGGTTATCC ACTTCATGTC TATCTTCTAA TGGGTTCATT CCTCCTAATT 
CTCGTCCTTG TCGGTCTATT CATCTTCTTC TGGCGTCGCT ACAAACTTGA ACAAGAGCTC 
GCCGCGATGA GCTGGAAGAT TCGATGGGAA GAGCTGGATG GTGAAGAGAG TCAAAAGAAG 
AATGAAAAGA AGAAGGCGAA GAAAAGGAAA AATCATAACG ATTATCTACC TGAATCTGAT 
CCATTATTAA GATCCACATC GAGGTCGTCA GTGAATTCGG ACAAGAGATC GTCCAGCTCG 
GGAACAACTC GTAAAATCTC AGCGATGATT GATCGAAAGT TGAGCATTTT CACGCGAAAA 
AAGAGCACAC CACCATCTGA ATCACAAAAG AACGGAGGAC TCACACCGAA TTCGCTTCAA 
AAAGCGGAAA ATGGAGATTG TTCACCGATT AATGAAGTTC AATTCAGACT TCCATTAAAT 
GATCGGAGAG TATCATCACC ATCATCAGAA GCGACGAGGA AAAAGAATAG TAATGAAGAA 
GATCCGGAGA ATGGTGCAAA GAAAAGTTTG AGCTTGAAAA ATCGAAAACT CAGCTTTGGA 
ATGGTTTCAT TCAAGAGTGG AAGTGGTGGA TCAGTGGAAA CAATTGCTCA AAATAATACG 
CAAATATACA CGAAAACGGC AATATTCAAG GGAGTTGTTG TGGCGATCAA GAAGTTGAAT 
ATAGATCCAA AGAAGTATCC ACGTCTTGAT TTATCACGTG CTCAATTGAT GGAATTAAAA 
AAGATGAAGG ATCTTCAACA TGATCATATT ACGAGATTCA CCGGAGCATG TATCGATTTT 
CCACATTATT GTGTAGTAAC TGAATACTGT CCGAAAGGTT CATTGGAAGA TATATTGGAA 
AATGAAAAAA TCGAATTGGA CAAACTGATG AAGTACTCAC TACTTCACGA TTTGGTTAAG 
GGTCTATTCT TCCTGCACAA TAGTGAAATT CGATCTCACG GACGTCTGAA ATCATCGAAT 
TGTGTCGTTG ACAGTCGATT TGTGCTGAAA GTCACCGATT TCGGACTTCA CAGGCTTCAT 
TGTTTAGAAG AAATTAATTT GGAAGAAATT GGAGAGCACG CGTATTATAA AAAAATGCTG 
TGGACGGCTC CAGAATTGCT CCGAGATTCG AATGCTCCAC CAATGGGAAC CCAGAAGGGT 
GATATCTATT CATTTGCAAT TATTTTGCAT GAAATGATGT TCCGGAAGGG AGTTTTCGCA 
TTGGAGAATG AGGATTTATC ACCGAATGAA ATAGTGCAAC GGGTACGAAA ACCAGTATCA 
GAAGATCAAG AACCACTTCG ACCGTGGGTA TCAGAAACTG GTGAAGGTGA AGGTGATGAT 
GCATTAAATG ATACATTATT ATCATTAATG GTTGCATGTT GGTCTGAAGA TCCACATGAA 
CGGCCTGAAG TATCATCTGT TCGAAAAGCG GTTCGAAGTT TGAATCGAGA TAATGAAACA 
TCGAATCTTG TGGATAATTT ACTCAAAAGA ATGGAACAAT ATGCTAATAA TTTGGAAGGT 
CTTGTTGAGG AACGCACGCA AGAATATTTG GCGGAAAAGA AAAAGGTAGA AGAACTTCTT 
CATCAACTTC TTCCACCTGC AATCGCCGAC ACTTTAATTG CTGGACGAGC TGTTCAAGCT 
GAATCGTATG ATTGTGTAAC TATCTACTTT TCTGATATTG TCGGATTCAC ATCACTATCA 
TCACAATCAA CACCAATGCA AGTGGTCACA TTACTCAATG ATTTATATTT GGCATTTGAT 
GGAGTTGTAG ACAATTTCAA AGTATATAAA GTTGAGACTA TTGGTGATGC ATACATGGTA 
GTATCTGGAT TACCAGAACG ACGAGATGAT CATGCTAATC AAATAGCTCA AATGTCACTA 
TCATTATTGC ATAAAGTTAA GAATTTTGTG ATTCGACATC GACCTCATGA ACAGCTCAAA 
CTTCGTATCG GAATGCATTC AGGATCCGTT GTGGCAGGAG TCGTAGGTTC AAAAATGCCT 
CGATACTGTC TTTTCGGAGA TACTGTGAAC ACTTCGAGTC GAATGGAAAG CAATGGATTA 
CCTCTCAAAA TCCACGTGTC ACAACAAACG TACGATATTC TGATGCAAGA AGCGGGCTTT 
AAACTCGAAC TACGAGGTTC AGTTGAGATG AAAGGCAAAG GAATGCAGAC TACTTATTGG 
TTACGAGGTT ACAAAGACGT TGAGATTCCC GATTTTGGCG AAGAATTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_001026429.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021600.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

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CloneID	OCa337136
Clone ID Related Accession (Same CDS sequence)	NM_001026429.2 , NM_001026429.3
Accession Version	NM_001026429.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001026429.1. COMPLETENESS: incomplete on both ends.

ATGCTCAGAT GGCTAACTCT ACTTTCATGT ATTCTCCTGA CCGCGCTGCA CGGAAATATC 
GTCGAAGACG TCGGCGCCGC ACAACAGGCT AGCTATCCAC CCCCACCACC ACCGTTCCCG 
TTCAATGTGA TTGTTATTCT GCCGAAGCGG GAGTCGACGT ACGACAATTT TGGAATGACT 
CTACAAAAGG CGATGCCAGT TATCGATATT GCCGTGCAAG AAGTTATCAA AGCGAAGAAG 
CTTCCTCCCG GATGGATCAA TTTGACATAT TGGGATTCTC GTCTTTATGA GGATATTCTT 
CTAGCTGAAC GACATGCGAC TGTTGGAGTT ATTCAAGCAT ACTGTGAACA TCGACTCGAC 
GCAATTCTTG GATTTGCTGA CAACTATGGA TTAGCTACAG TAACCAAGGT GACGGCGGGA 
CTGAATGGTG GTATCCCAAT ATTAACGACT TCTGGAATGC CATCCTTGTT AAATTCAAAG 
AAAGAATATC CATTTTTGAC TAGAATGCAA GGAAGTTACC GTCTTTTGGC TGATTCCATG 
TATCAACTAA TTGCCTATCA TGATGAGGAT TCGGTGTCCA AGTCGAATTC GTCTCTCAAC 
TACCTCAATT TAATATTTTT CTATCACGAT AAACGTCGTG CAGTCAATCG GGTAATTGCA 
CAAGATGAAA GTCAGGAGAC AGGTGCAACG TCGTCACATT GTTACTTCTC GCTGTACGCC 
ATTAAACGTT ATTTCACTGA AAAAAGTAAG ACGTTCAAGA GGGAATGGGC GCTGAACACT 
CCGCAATTTC CGTTCGACGA GGACTTGGTC ATCGAGAGGG AAACGTTTAA ACAGTGGCTG 
CGAGAAATTT CGTTACAGTC GAATGTGATT ATCCTATGTG CATCACCGGA TACTGTACGT 
GAAATAATGT TGGCTGCTCA TGATCTTGGA ATGGCGACAA GTGGTGAATA TGTATTCATT 
AATATCGATG TTTCAACGGG ATCACACGCC GAACAACCAT GGATTCGAGC AAATGACACG 
AATAATGAGG AGAACGAGAA AGCAAAAGAA GCATATCGAG CATTGAAAAC AATTTCATTG 
AGACGATCCG ATTTGGATGA ATATAAGAAT TTTGAACTTC GAGTCAAAGA ACGAGCAGAT 
CAAAAATATA ATTATACGAA TATCACCGGA AAAGATTATG AGATGAACAA TTTCATCAGT 
GCATTTTACG ATGCGGTTCT TCTCTATGCC ATTGCTCTAA ATGAGACAAT TCAATCAGGT 
CTTGATCCAC GAAATGGACA TAATATCACG TCACGAATGT GGGGACGAAC ATTTGTCGGA 
ATTACTGGAA ATGTATCGAT TGATCACAAT GGTGATAGGT ATTCGGATTA TTCTCTACTC 
GATTTGGATC CCGTTCAGAA TAGATTTGTG GAAGTAGCCT ACTACAGTGG AGCATCAAAT 
CAACTAAAAA CTGTGGGACA ACTGCATTGG GTTGGCGGAA AACCTCCAAC AGATCTACCT 
ATATGTGGTT ACGATAAGAG CAAGTGTCCG GGTTATCCAC TTCATGTCTA TCTTCTAATG 
GGTTCATTCC TCCTAATTCT CGTCCTTGTC GGTCTATTCA TCTTCTTCTG GCGTCGCTAC 
AAACTTGAAC AAGAGCTCGC CGCGATGAGC TGGAAGATTC GATGGGAAGA GCTGGATGGT 
GAAGAGAGTC AAAAGAAGAA TGAAAAGAAG AAGGCGAAGA AAAGGAAAAA TCATAACGAT 
TATCTACCTG AATCTGATCC ATTATTAAGA TCCACATCGA GGTCGTCAGT GAATTCGGAC 
AAGTTCGACG AGGACAGTTT GATTCCAATC AGATTTCGTC TGAGATCGTC CAGCTCGGGA 
ACAACTCGTA AAATCTCAGC GATGATTGAT CGAAAGTTGA GCATTTTCAC GCGAAAAAAG 
AGCACACCAC CATCTGAATC ACAAAAGAAC GGAGGACTCA CACCGAATTC GCTTCAAAAA 
GCGGAAAATG GAGATTGTTC ACCGATTAAT GAAGTTCAAT TCAGACTTCC ATTAAATGAT 
CGGAGAGTAT CATCACCATC ATCAGAAGCG ACGAGGAAAA AGAATAGTAA TGAAGAAGAT 
CCGGAGAATG GTGCAAAGAA AAGTTTGAGC TTGAAAAATC GAAAACTCAG CTTTGGAATG 
GTTTCATTCA AGAGTGGAAG TGGTGGATCA GTGGAAACAA TTGCTCAAAA TAATACGCAA 
ATATACACGA AAACGGCAAT ATTCAAGGGA GTTGTTGTGG CGATCAAGAA GTTGAATATA 
GATCCAAAGA AGTATCCACG TCTTGATTTA TCACGTGCTC AATTGATGGA ATTAAAAAAG 
ATGAAGGATC TTCAACATGA TCATATTACG AGATTCACCG GAGCATGTAT CGATTTTCCA 
CATTATTGTG TAGTAACTGA ATACTGTCCG AAAGGTTCAT TGGAAGATAT ATTGGAAAAT 
GAAAAAATCG AATTGGACAA ACTGATGAAG TACTCACTAC TTCACGATTT GGTTAAGGGT 
CTATTCTTCC TGCACAATAG TGAAATTCGA TCTCACGGAC GTCTGAAATC ATCGAATTGT 
GTCGTTGACA GTCGATTTGT GCTGAAAGTC ACCGATTTCG GACTTCACAG GCTTCATTGT 
TTAGAAGAAA TTAATTTGGA AGAAATTGGA GAGCACGCGT ATTATAAAAA AATGCTGTGG 
ACGGCTCCAG AATTGCTCCG AGATTCGAAT GCTCCACCAA TGGGAACCCA GAAGGGTGAT 
ATCTATTCAT TTGCAATTAT TTTGCATGAA ATGATGTTCC GGAAGGGAGT TTTCGCATTG 
GAGAATGAGG ATTTATCACC GAATGAAATA GTGCAACGGG TACGAAAACC AGTATCAGAA 
GATCAAGAAC CACTTCGACC GTGGGTATCA GAAACTGGTG AAGGTGAAGG TGATGATGCA 
TTAAATGATA CATTATTATC ATTAATGGTT GCATGTTGGT CTGAAGATCC ACATGAACGG 
CCTGAAGTAT CATCTGTTCG AAAAGCGGTT CGAAGTTTGA ATCGAGATAA TGAAACATCG 
AATCTTGTGG ATAATTTACT CAAAAGAATG GAACAATATG CTAATAATTT GGAAGGTCTT 
GTTGAGGAAC GCACGCAAGA ATATTTGGCG GAAAAGAAAA AGGTAGAAGA ACTTCTTCAT 
CAACTTCTTC CACCTGCAAT CGCCGACACT TTAATTGCTG GACGAGCTGT TCAAGCTGAA 
TCGTATGATT GTGTAACTAT CTACTTTTCT GATATTGTCG GATTCACATC ACTATCATCA 
CAATCAACAC CAATGCAAGT GGTCACATTA CTCAATGATT TATATTTGGC ATTTGATGGA 
GTTGTAGACA ATTTCAAAGT ATATAAAGTT GAGACTATTG GTGATGCATA CATGGTAGTA 
TCTGGATTAC CAGAACGACG AGATGATCAT GCTAATCAAA TAGCTCAAAT GTCACTATCA 
TTATTGCATA AAGTTAAGAA TTTTGTGATT CGACATCGAC CTCATGAACA GCTCAAACTT 
CGTATCGGAA TGCATTCAGG ATCCGTTGTG GCAGGAGTCG TAGGTTCAAA AATGCCTCGA 
TACTGTCTTT TCGGAGATAC TGTGAACACT TCGAGTCGAA TGGAAAGCAA TGGATTACCT 
CTCAAAATCC ACGTGTCACA ACAAACGTAC GATATTCTGA TGCAAGAAGC GGGCTTTAAA 
CTCGAACTAC GAGGTTCAGT TGAGATGAAA GGCAAAGGAA TGCAGACTAC TTATTGGTTA 
CGAGGTTACA AAGACGTTGA GATTCCCGAT TTTGGCGAAG AATTTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021600.1
CDS	1..3831
Translation	MLRWLTLLSCILLTALHGNIVEDVGAAQQASYPPPPPPFPFNVIVILPKRESTYDNFGMTLQKAMPVIDIAVQEVIKAKKLPPGWINLTYWDSRLYEDILLAERHATVGVIQAYCEHRLDAILGFADNYGLATVTKVTAGLNGGIPILTTSGMPSLLNSKKEYPFLTRMQGSYRLLADSMYQLIAYHDEDSVSKSNSSLNYLNLIFFYHDKRRAVNRVIAQDESQETGATSSHCYFSLYAIKRYFTEKSKTFKREWALNTPQFPFDEDLVIERETFKQWLREISLQSNVIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKFDEDSLIPIRFRLRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_001026429.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026429.2

ORF Insert Sequence:

ATGCTCAGAT GGCTAACTCT ACTTTCATGT ATTCTCCTGA CCGCGCTGCA CGGAAATATC 
GTCGAAGACG TCGGCGCCGC ACAACAGGCT AGCTATCCAC CCCCACCACC ACCGTTCCCG 
TTCAATGTGA TTGTTATTCT GCCGAAGCGG GAGTCGACGT ACGACAATTT TGGAATGACT 
CTACAAAAGG CGATGCCAGT TATCGATATT GCCGTGCAAG AAGTTATCAA AGCGAAGAAG 
CTTCCTCCCG GATGGATCAA TTTGACATAT TGGGATTCTC GTCTTTATGA GGATATTCTT 
CTAGCTGAAC GACATGCGAC TGTTGGAGTT ATTCAAGCAT ACTGTGAACA TCGACTCGAC 
GCAATTCTTG GATTTGCTGA CAACTATGGA TTAGCTACAG TAACCAAGGT GACGGCGGGA 
CTGAATGGTG GTATCCCAAT ATTAACGACT TCTGGAATGC CATCCTTGTT AAATTCAAAG 
AAAGAATATC CATTTTTGAC TAGAATGCAA GGAAGTTACC GTCTTTTGGC TGATTCCATG 
TATCAACTAA TTGCCTATCA TGATGAGGAT TCGGTGTCCA AGTCGAATTC GTCTCTCAAC 
TACCTCAATT TAATATTTTT CTATCACGAT AAACGTCGTG CAGTCAATCG GGTAATTGCA 
CAAGATGAAA GTCAGGAGAC AGGTGCAACG TCGTCACATT GTTACTTCTC GCTGTACGCC 
ATTAAACGTT ATTTCACTGA AAAAAGTAAG ACGTTCAAGA GGGAATGGGC GCTGAACACT 
CCGCAATTTC CGTTCGACGA GGACTTGGTC ATCGAGAGGG AAACGTTTAA ACAGTGGCTG 
CGAGAAATTT CGTTACAGTC GAATGTGATT ATCCTATGTG CATCACCGGA TACTGTACGT 
GAAATAATGT TGGCTGCTCA TGATCTTGGA ATGGCGACAA GTGGTGAATA TGTATTCATT 
AATATCGATG TTTCAACGGG ATCACACGCC GAACAACCAT GGATTCGAGC AAATGACACG 
AATAATGAGG AGAACGAGAA AGCAAAAGAA GCATATCGAG CATTGAAAAC AATTTCATTG 
AGACGATCCG ATTTGGATGA ATATAAGAAT TTTGAACTTC GAGTCAAAGA ACGAGCAGAT 
CAAAAATATA ATTATACGAA TATCACCGGA AAAGATTATG AGATGAACAA TTTCATCAGT 
GCATTTTACG ATGCGGTTCT TCTCTATGCC ATTGCTCTAA ATGAGACAAT TCAATCAGGT 
CTTGATCCAC GAAATGGACA TAATATCACG TCACGAATGT GGGGACGAAC ATTTGTCGGA 
ATTACTGGAA ATGTATCGAT TGATCACAAT GGTGATAGGT ATTCGGATTA TTCTCTACTC 
GATTTGGATC CCGTTCAGAA TAGATTTGTG GAAGTAGCCT ACTACAGTGG AGCATCAAAT 
CAACTAAAAA CTGTGGGACA ACTGCATTGG GTTGGCGGAA AACCTCCAAC AGATCTACCT 
ATATGTGGTT ACGATAAGAG CAAGTGTCCG GGTTATCCAC TTCATGTCTA TCTTCTAATG 
GGTTCATTCC TCCTAATTCT CGTCCTTGTC GGTCTATTCA TCTTCTTCTG GCGTCGCTAC 
AAACTTGAAC AAGAGCTCGC CGCGATGAGC TGGAAGATTC GATGGGAAGA GCTGGATGGT 
GAAGAGAGTC AAAAGAAGAA TGAAAAGAAG AAGGCGAAGA AAAGGAAAAA TCATAACGAT 
TATCTACCTG AATCTGATCC ATTATTAAGA TCCACATCGA GGTCGTCAGT GAATTCGGAC 
AAGTTCGACG AGGACAGTTT GATTCCAATC AGATTTCGTC TGAGATCGTC CAGCTCGGGA 
ACAACTCGTA AAATCTCAGC GATGATTGAT CGAAAGTTGA GCATTTTCAC GCGAAAAAAG 
AGCACACCAC CATCTGAATC ACAAAAGAAC GGAGGACTCA CACCGAATTC GCTTCAAAAA 
GCGGAAAATG GAGATTGTTC ACCGATTAAT GAAGTTCAAT TCAGACTTCC ATTAAATGAT 
CGGAGAGTAT CATCACCATC ATCAGAAGCG ACGAGGAAAA AGAATAGTAA TGAAGAAGAT 
CCGGAGAATG GTGCAAAGAA AAGTTTGAGC TTGAAAAATC GAAAACTCAG CTTTGGAATG 
GTTTCATTCA AGAGTGGAAG TGGTGGATCA GTGGAAACAA TTGCTCAAAA TAATACGCAA 
ATATACACGA AAACGGCAAT ATTCAAGGGA GTTGTTGTGG CGATCAAGAA GTTGAATATA 
GATCCAAAGA AGTATCCACG TCTTGATTTA TCACGTGCTC AATTGATGGA ATTAAAAAAG 
ATGAAGGATC TTCAACATGA TCATATTACG AGATTCACCG GAGCATGTAT CGATTTTCCA 
CATTATTGTG TAGTAACTGA ATACTGTCCG AAAGGTTCAT TGGAAGATAT ATTGGAAAAT 
GAAAAAATCG AATTGGACAA ACTGATGAAG TACTCACTAC TTCACGATTT GGTTAAGGGT 
CTATTCTTCC TGCACAATAG TGAAATTCGA TCTCACGGAC GTCTGAAATC ATCGAATTGT 
GTCGTTGACA GTCGATTTGT GCTGAAAGTC ACCGATTTCG GACTTCACAG GCTTCATTGT 
TTAGAAGAAA TTAATTTGGA AGAAATTGGA GAGCACGCGT ATTATAAAAA AATGCTGTGG 
ACGGCTCCAG AATTGCTCCG AGATTCGAAT GCTCCACCAA TGGGAACCCA GAAGGGTGAT 
ATCTATTCAT TTGCAATTAT TTTGCATGAA ATGATGTTCC GGAAGGGAGT TTTCGCATTG 
GAGAATGAGG ATTTATCACC GAATGAAATA GTGCAACGGG TACGAAAACC AGTATCAGAA 
GATCAAGAAC CACTTCGACC GTGGGTATCA GAAACTGGTG AAGGTGAAGG TGATGATGCA 
TTAAATGATA CATTATTATC ATTAATGGTT GCATGTTGGT CTGAAGATCC ACATGAACGG 
CCTGAAGTAT CATCTGTTCG AAAAGCGGTT CGAAGTTTGA ATCGAGATAA TGAAACATCG 
AATCTTGTGG ATAATTTACT CAAAAGAATG GAACAATATG CTAATAATTT GGAAGGTCTT 
GTTGAGGAAC GCACGCAAGA ATATTTGGCG GAAAAGAAAA AGGTAGAAGA ACTTCTTCAT 
CAACTTCTTC CACCTGCAAT CGCCGACACT TTAATTGCTG GACGAGCTGT TCAAGCTGAA 
TCGTATGATT GTGTAACTAT CTACTTTTCT GATATTGTCG GATTCACATC ACTATCATCA 
CAATCAACAC CAATGCAAGT GGTCACATTA CTCAATGATT TATATTTGGC ATTTGATGGA 
GTTGTAGACA ATTTCAAAGT ATATAAAGTT GAGACTATTG GTGATGCATA CATGGTAGTA 
TCTGGATTAC CAGAACGACG AGATGATCAT GCTAATCAAA TAGCTCAAAT GTCACTATCA 
TTATTGCATA AAGTTAAGAA TTTTGTGATT CGACATCGAC CTCATGAACA GCTCAAACTT 
CGTATCGGAA TGCATTCAGG ATCCGTTGTG GCAGGAGTCG TAGGTTCAAA AATGCCTCGA 
TACTGTCTTT TCGGAGATAC TGTGAACACT TCGAGTCGAA TGGAAAGCAA TGGATTACCT 
CTCAAAATCC ACGTGTCACA ACAAACGTAC GATATTCTGA TGCAAGAAGC GGGCTTTAAA 
CTCGAACTAC GAGGTTCAGT TGAGATGAAA GGCAAAGGAA TGCAGACTAC TTATTGGTTA 
CGAGGTTACA AAGACGTTGA GATTCCCGAT TTTGGCGAAG AATTTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_001026429.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001021600.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Price & Availability↑

CloneID	OCa337136
Clone ID Related Accession (Same CDS sequence)	NM_001026429.2 , NM_001026429.3
Accession Version	NM_001026429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001026429.2.

ATGCTCAGAT GGCTAACTCT ACTTTCATGT ATTCTCCTGA CCGCGCTGCA CGGAAATATC 
GTCGAAGACG TCGGCGCCGC ACAACAGGCT AGCTATCCAC CCCCACCACC ACCGTTCCCG 
TTCAATGTGA TTGTTATTCT GCCGAAGCGG GAGTCGACGT ACGACAATTT TGGAATGACT 
CTACAAAAGG CGATGCCAGT TATCGATATT GCCGTGCAAG AAGTTATCAA AGCGAAGAAG 
CTTCCTCCCG GATGGATCAA TTTGACATAT TGGGATTCTC GTCTTTATGA GGATATTCTT 
CTAGCTGAAC GACATGCGAC TGTTGGAGTT ATTCAAGCAT ACTGTGAACA TCGACTCGAC 
GCAATTCTTG GATTTGCTGA CAACTATGGA TTAGCTACAG TAACCAAGGT GACGGCGGGA 
CTGAATGGTG GTATCCCAAT ATTAACGACT TCTGGAATGC CATCCTTGTT AAATTCAAAG 
AAAGAATATC CATTTTTGAC TAGAATGCAA GGAAGTTACC GTCTTTTGGC TGATTCCATG 
TATCAACTAA TTGCCTATCA TGATGAGGAT TCGGTGTCCA AGTCGAATTC GTCTCTCAAC 
TACCTCAATT TAATATTTTT CTATCACGAT AAACGTCGTG CAGTCAATCG GGTAATTGCA 
CAAGATGAAA GTCAGGAGAC AGGTGCAACG TCGTCACATT GTTACTTCTC GCTGTACGCC 
ATTAAACGTT ATTTCACTGA AAAAAGTAAG ACGTTCAAGA GGGAATGGGC GCTGAACACT 
CCGCAATTTC CGTTCGACGA GGACTTGGTC ATCGAGAGGG AAACGTTTAA ACAGTGGCTG 
CGAGAAATTT CGTTACAGTC GAATGTGATT ATCCTATGTG CATCACCGGA TACTGTACGT 
GAAATAATGT TGGCTGCTCA TGATCTTGGA ATGGCGACAA GTGGTGAATA TGTATTCATT 
AATATCGATG TTTCAACGGG ATCACACGCC GAACAACCAT GGATTCGAGC AAATGACACG 
AATAATGAGG AGAACGAGAA AGCAAAAGAA GCATATCGAG CATTGAAAAC AATTTCATTG 
AGACGATCCG ATTTGGATGA ATATAAGAAT TTTGAACTTC GAGTCAAAGA ACGAGCAGAT 
CAAAAATATA ATTATACGAA TATCACCGGA AAAGATTATG AGATGAACAA TTTCATCAGT 
GCATTTTACG ATGCGGTTCT TCTCTATGCC ATTGCTCTAA ATGAGACAAT TCAATCAGGT 
CTTGATCCAC GAAATGGACA TAATATCACG TCACGAATGT GGGGACGAAC ATTTGTCGGA 
ATTACTGGAA ATGTATCGAT TGATCACAAT GGTGATAGGT ATTCGGATTA TTCTCTACTC 
GATTTGGATC CCGTTCAGAA TAGATTTGTG GAAGTAGCCT ACTACAGTGG AGCATCAAAT 
CAACTAAAAA CTGTGGGACA ACTGCATTGG GTTGGCGGAA AACCTCCAAC AGATCTACCT 
ATATGTGGTT ACGATAAGAG CAAGTGTCCG GGTTATCCAC TTCATGTCTA TCTTCTAATG 
GGTTCATTCC TCCTAATTCT CGTCCTTGTC GGTCTATTCA TCTTCTTCTG GCGTCGCTAC 
AAACTTGAAC AAGAGCTCGC CGCGATGAGC TGGAAGATTC GATGGGAAGA GCTGGATGGT 
GAAGAGAGTC AAAAGAAGAA TGAAAAGAAG AAGGCGAAGA AAAGGAAAAA TCATAACGAT 
TATCTACCTG AATCTGATCC ATTATTAAGA TCCACATCGA GGTCGTCAGT GAATTCGGAC 
AAGTTCGACG AGGACAGTTT GATTCCAATC AGATTTCGTC TGAGATCGTC CAGCTCGGGA 
ACAACTCGTA AAATCTCAGC GATGATTGAT CGAAAGTTGA GCATTTTCAC GCGAAAAAAG 
AGCACACCAC CATCTGAATC ACAAAAGAAC GGAGGACTCA CACCGAATTC GCTTCAAAAA 
GCGGAAAATG GAGATTGTTC ACCGATTAAT GAAGTTCAAT TCAGACTTCC ATTAAATGAT 
CGGAGAGTAT CATCACCATC ATCAGAAGCG ACGAGGAAAA AGAATAGTAA TGAAGAAGAT 
CCGGAGAATG GTGCAAAGAA AAGTTTGAGC TTGAAAAATC GAAAACTCAG CTTTGGAATG 
GTTTCATTCA AGAGTGGAAG TGGTGGATCA GTGGAAACAA TTGCTCAAAA TAATACGCAA 
ATATACACGA AAACGGCAAT ATTCAAGGGA GTTGTTGTGG CGATCAAGAA GTTGAATATA 
GATCCAAAGA AGTATCCACG TCTTGATTTA TCACGTGCTC AATTGATGGA ATTAAAAAAG 
ATGAAGGATC TTCAACATGA TCATATTACG AGATTCACCG GAGCATGTAT CGATTTTCCA 
CATTATTGTG TAGTAACTGA ATACTGTCCG AAAGGTTCAT TGGAAGATAT ATTGGAAAAT 
GAAAAAATCG AATTGGACAA ACTGATGAAG TACTCACTAC TTCACGATTT GGTTAAGGGT 
CTATTCTTCC TGCACAATAG TGAAATTCGA TCTCACGGAC GTCTGAAATC ATCGAATTGT 
GTCGTTGACA GTCGATTTGT GCTGAAAGTC ACCGATTTCG GACTTCACAG GCTTCATTGT 
TTAGAAGAAA TTAATTTGGA AGAAATTGGA GAGCACGCGT ATTATAAAAA AATGCTGTGG 
ACGGCTCCAG AATTGCTCCG AGATTCGAAT GCTCCACCAA TGGGAACCCA GAAGGGTGAT 
ATCTATTCAT TTGCAATTAT TTTGCATGAA ATGATGTTCC GGAAGGGAGT TTTCGCATTG 
GAGAATGAGG ATTTATCACC GAATGAAATA GTGCAACGGG TACGAAAACC AGTATCAGAA 
GATCAAGAAC CACTTCGACC GTGGGTATCA GAAACTGGTG AAGGTGAAGG TGATGATGCA 
TTAAATGATA CATTATTATC ATTAATGGTT GCATGTTGGT CTGAAGATCC ACATGAACGG 
CCTGAAGTAT CATCTGTTCG AAAAGCGGTT CGAAGTTTGA ATCGAGATAA TGAAACATCG 
AATCTTGTGG ATAATTTACT CAAAAGAATG GAACAATATG CTAATAATTT GGAAGGTCTT 
GTTGAGGAAC GCACGCAAGA ATATTTGGCG GAAAAGAAAA AGGTAGAAGA ACTTCTTCAT 
CAACTTCTTC CACCTGCAAT CGCCGACACT TTAATTGCTG GACGAGCTGT TCAAGCTGAA 
TCGTATGATT GTGTAACTAT CTACTTTTCT GATATTGTCG GATTCACATC ACTATCATCA 
CAATCAACAC CAATGCAAGT GGTCACATTA CTCAATGATT TATATTTGGC ATTTGATGGA 
GTTGTAGACA ATTTCAAAGT ATATAAAGTT GAGACTATTG GTGATGCATA CATGGTAGTA 
TCTGGATTAC CAGAACGACG AGATGATCAT GCTAATCAAA TAGCTCAAAT GTCACTATCA 
TTATTGCATA AAGTTAAGAA TTTTGTGATT CGACATCGAC CTCATGAACA GCTCAAACTT 
CGTATCGGAA TGCATTCAGG ATCCGTTGTG GCAGGAGTCG TAGGTTCAAA AATGCCTCGA 
TACTGTCTTT TCGGAGATAC TGTGAACACT TCGAGTCGAA TGGAAAGCAA TGGATTACCT 
CTCAAAATCC ACGTGTCACA ACAAACGTAC GATATTCTGA TGCAAGAAGC GGGCTTTAAA 
CTCGAACTAC GAGGTTCAGT TGAGATGAAA GGCAAAGGAA TGCAGACTAC TTATTGGTTA 
CGAGGTTACA AAGACGTTGA GATTCCCGAT TTTGGCGAAG AATTTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001021600.1
CDS	33..3863
Translation	MLRWLTLLSCILLTALHGNIVEDVGAAQQASYPPPPPPFPFNVIVILPKRESTYDNFGMTLQKAMPVIDIAVQEVIKAKKLPPGWINLTYWDSRLYEDILLAERHATVGVIQAYCEHRLDAILGFADNYGLATVTKVTAGLNGGIPILTTSGMPSLLNSKKEYPFLTRMQGSYRLLADSMYQLIAYHDEDSVSKSNSSLNYLNLIFFYHDKRRAVNRVIAQDESQETGATSSHCYFSLYAIKRYFTEKSKTFKREWALNTPQFPFDEDLVIERETFKQWLREISLQSNVIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKFDEDSLIPIRFRLRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_001026429.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001026429.3

ORF Insert Sequence:

ATGCTCAGAT GGCTAACTCT ACTTTCATGT ATTCTCCTGA CCGCGCTGCA CGGAAATATC 
GTCGAAGACG TCGGCGCCGC ACAACAGGCT AGCTATCCAC CCCCACCACC ACCGTTCCCG 
TTCAATGTGA TTGTTATTCT GCCGAAGCGG GAGTCGACGT ACGACAATTT TGGAATGACT 
CTACAAAAGG CGATGCCAGT TATCGATATT GCCGTGCAAG AAGTTATCAA AGCGAAGAAG 
CTTCCTCCCG GATGGATCAA TTTGACATAT TGGGATTCTC GTCTTTATGA GGATATTCTT 
CTAGCTGAAC GACATGCGAC TGTTGGAGTT ATTCAAGCAT ACTGTGAACA TCGACTCGAC 
GCAATTCTTG GATTTGCTGA CAACTATGGA TTAGCTACAG TAACCAAGGT GACGGCGGGA 
CTGAATGGTG GTATCCCAAT ATTAACGACT TCTGGAATGC CATCCTTGTT AAATTCAAAG 
AAAGAATATC CATTTTTGAC TAGAATGCAA GGAAGTTACC GTCTTTTGGC TGATTCCATG 
TATCAACTAA TTGCCTATCA TGATGAGGAT TCGGTGTCCA AGTCGAATTC GTCTCTCAAC 
TACCTCAATT TAATATTTTT CTATCACGAT AAACGTCGTG CAGTCAATCG GGTAATTGCA 
CAAGATGAAA GTCAGGAGAC AGGTGCAACG TCGTCACATT GTTACTTCTC GCTGTACGCC 
ATTAAACGTT ATTTCACTGA AAAAAGTAAG ACGTTCAAGA GGGAATGGGC GCTGAACACT 
CCGCAATTTC CGTTCGACGA GGACTTGGTC ATCGAGAGGG AAACGTTTAA ACAGTGGCTG 
CGAGAAATTT CGTTACAGTC GAATGTGATT ATCCTATGTG CATCACCGGA TACTGTACGT 
GAAATAATGT TGGCTGCTCA TGATCTTGGA ATGGCGACAA GTGGTGAATA TGTATTCATT 
AATATCGATG TTTCAACGGG ATCACACGCC GAACAACCAT GGATTCGAGC AAATGACACG 
AATAATGAGG AGAACGAGAA AGCAAAAGAA GCATATCGAG CATTGAAAAC AATTTCATTG 
AGACGATCCG ATTTGGATGA ATATAAGAAT TTTGAACTTC GAGTCAAAGA ACGAGCAGAT 
CAAAAATATA ATTATACGAA TATCACCGGA AAAGATTATG AGATGAACAA TTTCATCAGT 
GCATTTTACG ATGCGGTTCT TCTCTATGCC ATTGCTCTAA ATGAGACAAT TCAATCAGGT 
CTTGATCCAC GAAATGGACA TAATATCACG TCACGAATGT GGGGACGAAC ATTTGTCGGA 
ATTACTGGAA ATGTATCGAT TGATCACAAT GGTGATAGGT ATTCGGATTA TTCTCTACTC 
GATTTGGATC CCGTTCAGAA TAGATTTGTG GAAGTAGCCT ACTACAGTGG AGCATCAAAT 
CAACTAAAAA CTGTGGGACA ACTGCATTGG GTTGGCGGAA AACCTCCAAC AGATCTACCT 
ATATGTGGTT ACGATAAGAG CAAGTGTCCG GGTTATCCAC TTCATGTCTA TCTTCTAATG 
GGTTCATTCC TCCTAATTCT CGTCCTTGTC GGTCTATTCA TCTTCTTCTG GCGTCGCTAC 
AAACTTGAAC AAGAGCTCGC CGCGATGAGC TGGAAGATTC GATGGGAAGA GCTGGATGGT 
GAAGAGAGTC AAAAGAAGAA TGAAAAGAAG AAGGCGAAGA AAAGGAAAAA TCATAACGAT 
TATCTACCTG AATCTGATCC ATTATTAAGA TCCACATCGA GGTCGTCAGT GAATTCGGAC 
AAGTTCGACG AGGACAGTTT GATTCCAATC AGATTTCGTC TGAGATCGTC CAGCTCGGGA 
ACAACTCGTA AAATCTCAGC GATGATTGAT CGAAAGTTGA GCATTTTCAC GCGAAAAAAG 
AGCACACCAC CATCTGAATC ACAAAAGAAC GGAGGACTCA CACCGAATTC GCTTCAAAAA 
GCGGAAAATG GAGATTGTTC ACCGATTAAT GAAGTTCAAT TCAGACTTCC ATTAAATGAT 
CGGAGAGTAT CATCACCATC ATCAGAAGCG ACGAGGAAAA AGAATAGTAA TGAAGAAGAT 
CCGGAGAATG GTGCAAAGAA AAGTTTGAGC TTGAAAAATC GAAAACTCAG CTTTGGAATG 
GTTTCATTCA AGAGTGGAAG TGGTGGATCA GTGGAAACAA TTGCTCAAAA TAATACGCAA 
ATATACACGA AAACGGCAAT ATTCAAGGGA GTTGTTGTGG CGATCAAGAA GTTGAATATA 
GATCCAAAGA AGTATCCACG TCTTGATTTA TCACGTGCTC AATTGATGGA ATTAAAAAAG 
ATGAAGGATC TTCAACATGA TCATATTACG AGATTCACCG GAGCATGTAT CGATTTTCCA 
CATTATTGTG TAGTAACTGA ATACTGTCCG AAAGGTTCAT TGGAAGATAT ATTGGAAAAT 
GAAAAAATCG AATTGGACAA ACTGATGAAG TACTCACTAC TTCACGATTT GGTTAAGGGT 
CTATTCTTCC TGCACAATAG TGAAATTCGA TCTCACGGAC GTCTGAAATC ATCGAATTGT 
GTCGTTGACA GTCGATTTGT GCTGAAAGTC ACCGATTTCG GACTTCACAG GCTTCATTGT 
TTAGAAGAAA TTAATTTGGA AGAAATTGGA GAGCACGCGT ATTATAAAAA AATGCTGTGG 
ACGGCTCCAG AATTGCTCCG AGATTCGAAT GCTCCACCAA TGGGAACCCA GAAGGGTGAT 
ATCTATTCAT TTGCAATTAT TTTGCATGAA ATGATGTTCC GGAAGGGAGT TTTCGCATTG 
GAGAATGAGG ATTTATCACC GAATGAAATA GTGCAACGGG TACGAAAACC AGTATCAGAA 
GATCAAGAAC CACTTCGACC GTGGGTATCA GAAACTGGTG AAGGTGAAGG TGATGATGCA 
TTAAATGATA CATTATTATC ATTAATGGTT GCATGTTGGT CTGAAGATCC ACATGAACGG 
CCTGAAGTAT CATCTGTTCG AAAAGCGGTT CGAAGTTTGA ATCGAGATAA TGAAACATCG 
AATCTTGTGG ATAATTTACT CAAAAGAATG GAACAATATG CTAATAATTT GGAAGGTCTT 
GTTGAGGAAC GCACGCAAGA ATATTTGGCG GAAAAGAAAA AGGTAGAAGA ACTTCTTCAT 
CAACTTCTTC CACCTGCAAT CGCCGACACT TTAATTGCTG GACGAGCTGT TCAAGCTGAA 
TCGTATGATT GTGTAACTAT CTACTTTTCT GATATTGTCG GATTCACATC ACTATCATCA 
CAATCAACAC CAATGCAAGT GGTCACATTA CTCAATGATT TATATTTGGC ATTTGATGGA 
GTTGTAGACA ATTTCAAAGT ATATAAAGTT GAGACTATTG GTGATGCATA CATGGTAGTA 
TCTGGATTAC CAGAACGACG AGATGATCAT GCTAATCAAA TAGCTCAAAT GTCACTATCA 
TTATTGCATA AAGTTAAGAA TTTTGTGATT CGACATCGAC CTCATGAACA GCTCAAACTT 
CGTATCGGAA TGCATTCAGG ATCCGTTGTG GCAGGAGTCG TAGGTTCAAA AATGCCTCGA 
TACTGTCTTT TCGGAGATAC TGTGAACACT TCGAGTCGAA TGGAAAGCAA TGGATTACCT 
CTCAAAATCC ACGTGTCACA ACAAACGTAC GATATTCTGA TGCAAGAAGC GGGCTTTAAA 
CTCGAACTAC GAGGTTCAGT TGAGATGAAA GGCAAAGGAA TGCAGACTAC TTATTGGTTA 
CGAGGTTACA AAGACGTTGA GATTCCCGAT TTTGGCGAAG AATTTGCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_001262699.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001249628.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

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CloneID	OCa337137
Clone ID Related Accession (Same CDS sequence)	NM_001262699.2 , NM_001262699.1
Accession Version	NM_001262699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001262699.1.

ATGTGGATTA ACAGCACGCG AACACTTATA TTTAATCATT TCGTACGCAT TGTACTGGTG 
CTTCTTCTGT CTCACGAGGT CGTCACAAAG TCTAATAGAA AATTTGAGGA GATCTCTGTC 
AAGCATCCGA TCCATTTGGT CATTCCATTG CCAGACGATG ATGATTTTAG TGATGGAAAA 
AATCCATTTT TGTTCTCATC TCACAAAGTA AAACCGCTCG CTGATTTGGC TCTGGAGAGA 
GTTTATCGAG AAGGAATCCT ACCCAACAAC TCTGTAAATC TAATTTATCG AGACTCAAAA 
CTATCGGATG CTATTGGTCC AAACGTGGCA GTTGAGCAAC TTCTTCGAAA ACAAATTGAT 
TGTATAATTG GTTATGCGTA TGGATATGCA CTTGCACCAG TTGCCAGAAT GTCTCCATAT 
TGGAAAAATG GAATTCCGAT AATCACACCA ATTGGCTTGA CAATGAGTTT AGATGATAAA 
CGAGAATATC AGTTAATGAC AAGAATTAAT AGTCCGTATA AAGTAGTCTC CAGCGCTGTA 
TCAACACTAT TCAACACATA TAAATGGAAA CGCCACATTT TTATGTTTCA CCACGCAAAA 
GCGCCGTCTG TGGCCGTTGG AGAATGCTTC CTTCTGATGG CAAGTCTACA GCATCCACTT 
CGAAAGGTCA TGGAAATGCA ACACAACTTC TTCACGTTCA ACGAAGATCA CGGAGCGAAT 
ATATCAAAAG CGGAACGGCA CAATCAATTT CGGAACTATT TGCGAGCGAG CAGTAATATG 
GCGAATGTGA TTATCCTATG TGCATCACCG GATACTGTAC GTGAAATAAT GTTGGCTGCT 
CATGATCTTG GAATGGCGAC AAGTGGTGAA TATGTATTCA TTAATATCGA TGTTTCAACG 
GGATCACACG CCGAACAACC ATGGATTCGA GCAAATGACA CGAATAATGA GGAGAACGAG 
AAAGCAAAAG AAGCATATCG AGCATTGAAA ACAATTTCAT TGAGACGATC CGATTTGGAT 
GAATATAAGA ATTTTGAACT TCGAGTCAAA GAACGAGCAG ATCAAAAATA TAATTATACG 
AATATCACCG GAAAAGATTA TGAGATGAAC AATTTCATCA GTGCATTTTA CGATGCGGTT 
CTTCTCTATG CCATTGCTCT AAATGAGACA ATTCAATCAG GTCTTGATCC ACGAAATGGA 
CATAATATCA CGTCACGAAT GTGGGGACGA ACATTTGTCG GAATTACTGG AAATGTATCG 
ATTGATCACA ATGGTGATAG GTATTCGGAT TATTCTCTAC TCGATTTGGA TCCCGTTCAG 
AATAGATTTG TGGAAGTAGC CTACTACAGT GGAGCATCAA ATCAACTAAA AACTGTGGGA 
CAACTGCATT GGGTTGGCGG AAAACCTCCA ACAGATCTAC CTATATGTGG TTACGATAAG 
AGCAAGTGTC CGCAGGGTTA TCCACTTCAT GTCTATCTTC TAATGGGTTC ATTCCTCCTA 
ATTCTCGTCC TTGTCGGTCT ATTCATCTTC TTCTGGCGTC GCTACAAACT TGAACAAGAG 
CTCGCCGCGA TGAGCTGGAA GATTCGATGG GAAGAGCTGG ATGGTGAAGA GAGTCAAAAG 
AAGAATGAAA AGAAGAAGGC GAAGAAAAGG AAAAATCATA ACGATTATCT ACCTGAATCT 
GATCCATTAT TAAGATCCAC ATCGAGGTCG TCAGTGAATT CGGACAAGAG ATCGTCCAGC 
TCGGGAACAA CTCGTAAAAT CTCAGCGATG ATTGATCGAA AGTTGAGCAT TTTCACGCGA 
AAAAAGAGCA CACCACCATC TGAATCACAA AAGAACGGAG GACTCACACC GAATTCGCTT 
CAAAAAGCGG AAAATGGAGA TTGTTCACCG ATTAATGAAG TTCAATTCAG ACTTCCATTA 
AATGATCGGA GAGTATCATC ACCATCATCA GAAGCGACGA GGAAAAAGAA TAGTAATGAA 
GAAGATCCGG AGAATGGTGC AAAGAAAAGT TTGAGCTTGA AAAATCGAAA ACTCAGCTTT 
GGAATGGTTT CATTCAAGAG TGGAAGTGGT GGATCAGTGG AAACAATTGC TCAAAATAAT 
ACGCAAATAT ACACGAAAAC GGCAATATTC AAGGGAGTTG TTGTGGCGAT CAAGAAGTTG 
AATATAGATC CAAAGAAGTA TCCACGTCTT GATTTATCAC GTGCTCAATT GATGGAATTA 
AAAAAGATGA AGGATCTTCA ACATGATCAT ATTACGAGAT TCACCGGAGC ATGTATCGAT 
TTTCCACATT ATTGTGTAGT AACTGAATAC TGTCCGAAAG GTTCATTGGA AGATATATTG 
GAAAATGAAA AAATCGAATT GGACAAACTG ATGAAGTACT CACTACTTCA CGATTTGGTT 
AAGGGTCTAT TCTTCCTGCA CAATAGTGAA ATTCGATCTC ACGGACGTCT GAAATCATCG 
AATTGTGTCG TTGACAGTCG ATTTGTGCTG AAAGTCACCG ATTTCGGACT TCACAGGCTT 
CATTGTTTAG AAGAAATTAA TTTGGAAGAA ATTGGAGAGC ACGCGTATTA TAAAAAAATG 
CTGTGGACGG CTCCAGAATT GCTCCGAGAT TCGAATGCTC CACCAATGGG AACCCAGAAG 
GGTGATATCT ATTCATTTGC AATTATTTTG CATGAAATGA TGTTCCGGAA GGGAGTTTTC 
GCATTGGAGA ATGAGGATTT ATCACCGAAT GAAATAGTGC AACGGGTACG AAAACCAGTA 
TCAGAAGATC AAGAACCACT TCGACCGTGG GTATCAGAAA CTGGTGAAGG TGAAGGTGAT 
GATGCATTAA ATGATACATT ATTATCATTA ATGGTTGCAT GTTGGTCTGA AGATCCACAT 
GAACGGCCTG AAGTATCATC TGTTCGAAAA GCGGTTCGAA GTTTGAATCG AGATAATGAA 
ACATCGAATC TTGTGGATAA TTTACTCAAA AGAATGGAAC AATATGCTAA TAATTTGGAA 
GGTCTTGTTG AGGAACGCAC GCAAGAATAT TTGGCGGAAA AGAAAAAGGT AGAAGAACTT 
CTTCATCAAC TTCTTCCACC TGCAATCGCC GACACTTTAA TTGCTGGACG AGCTGTTCAA 
GCTGAATCGT ATGATTGTGT AACTATCTAC TTTTCTGATA TTGTCGGATT CACATCACTA 
TCATCACAAT CAACACCAAT GCAAGTGGTC ACATTACTCA ATGATTTATA TTTGGCATTT 
GATGGAGTTG TAGACAATTT CAAAGTATAT AAAGTTGAGA CTATTGGTGA TGCATACATG 
GTAGTATCTG GATTACCAGA ACGACGAGAT GATCATGCTA ATCAAATAGC TCAAATGTCA 
CTATCATTAT TGCATAAAGT TAAGAATTTT GTGATTCGAC ATCGACCTCA TGAACAGCTC 
AAACTTCGTA TCGGAATGCA TTCAGGATCC GTTGTGGCAG GAGTCGTAGG TTCAAAAATG 
CCTCGATACT GTCTTTTCGG AGATACTGTG AACACTTCGA GTCGAATGGA AAGCAATGGA 
TTACCTCTCA AAATCCACGT GTCACAACAA ACGTACGATA TTCTGATGCA AGAAGCGGGC 
TTTAAACTCG AACTACGAGG TTCAGTTGAG ATGAAAGGCA AAGGAATGCA GACTACTTAT 
TGGTTACGAG GTTACAAAGA CGTTGAGATT CCCGATTTTG GCGAAGAATT TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001249628.1
CDS	41..3757
Translation	MWINSTRTLIFNHFVRIVLVLLLSHEVVTKSNRKFEEISVKHPIHLVIPLPDDDDFSDGKNPFLFSSHKVKPLADLALERVYREGILPNNSVNLIYRDSKLSDAIGPNVAVEQLLRKQIDCIIGYAYGYALAPVARMSPYWKNGIPIITPIGLTMSLDDKREYQLMTRINSPYKVVSSAVSTLFNTYKWKRHIFMFHHAKAPSVAVGECFLLMASLQHPLRKVMEMQHNFFTFNEDHGANISKAERHNQFRNYLRASSNMANVIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPQGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_001262699.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001262699.2

ORF Insert Sequence:

ATGTGGATTA ACAGCACGCG AACACTTATA TTTAATCATT TCGTACGCAT TGTACTGGTG 
CTTCTTCTGT CTCACGAGGT CGTCACAAAG TCTAATAGAA AATTTGAGGA GATCTCTGTC 
AAGCATCCGA TCCATTTGGT CATTCCATTG CCAGACGATG ATGATTTTAG TGATGGAAAA 
AATCCATTTT TGTTCTCATC TCACAAAGTA AAACCGCTCG CTGATTTGGC TCTGGAGAGA 
GTTTATCGAG AAGGAATCCT ACCCAACAAC TCTGTAAATC TAATTTATCG AGACTCAAAA 
CTATCGGATG CTATTGGTCC AAACGTGGCA GTTGAGCAAC TTCTTCGAAA ACAAATTGAT 
TGTATAATTG GTTATGCGTA TGGATATGCA CTTGCACCAG TTGCCAGAAT GTCTCCATAT 
TGGAAAAATG GAATTCCGAT AATCACACCA ATTGGCTTGA CAATGAGTTT AGATGATAAA 
CGAGAATATC AGTTAATGAC AAGAATTAAT AGTCCGTATA AAGTAGTCTC CAGCGCTGTA 
TCAACACTAT TCAACACATA TAAATGGAAA CGCCACATTT TTATGTTTCA CCACGCAAAA 
GCGCCGTCTG TGGCCGTTGG AGAATGCTTC CTTCTGATGG CAAGTCTACA GCATCCACTT 
CGAAAGGTCA TGGAAATGCA ACACAACTTC TTCACGTTCA ACGAAGATCA CGGAGCGAAT 
ATATCAAAAG CGGAACGGCA CAATCAATTT CGGAACTATT TGCGAGCGAG CAGTAATATG 
GCGAATGTGA TTATCCTATG TGCATCACCG GATACTGTAC GTGAAATAAT GTTGGCTGCT 
CATGATCTTG GAATGGCGAC AAGTGGTGAA TATGTATTCA TTAATATCGA TGTTTCAACG 
GGATCACACG CCGAACAACC ATGGATTCGA GCAAATGACA CGAATAATGA GGAGAACGAG 
AAAGCAAAAG AAGCATATCG AGCATTGAAA ACAATTTCAT TGAGACGATC CGATTTGGAT 
GAATATAAGA ATTTTGAACT TCGAGTCAAA GAACGAGCAG ATCAAAAATA TAATTATACG 
AATATCACCG GAAAAGATTA TGAGATGAAC AATTTCATCA GTGCATTTTA CGATGCGGTT 
CTTCTCTATG CCATTGCTCT AAATGAGACA ATTCAATCAG GTCTTGATCC ACGAAATGGA 
CATAATATCA CGTCACGAAT GTGGGGACGA ACATTTGTCG GAATTACTGG AAATGTATCG 
ATTGATCACA ATGGTGATAG GTATTCGGAT TATTCTCTAC TCGATTTGGA TCCCGTTCAG 
AATAGATTTG TGGAAGTAGC CTACTACAGT GGAGCATCAA ATCAACTAAA AACTGTGGGA 
CAACTGCATT GGGTTGGCGG AAAACCTCCA ACAGATCTAC CTATATGTGG TTACGATAAG 
AGCAAGTGTC CGCAGGGTTA TCCACTTCAT GTCTATCTTC TAATGGGTTC ATTCCTCCTA 
ATTCTCGTCC TTGTCGGTCT ATTCATCTTC TTCTGGCGTC GCTACAAACT TGAACAAGAG 
CTCGCCGCGA TGAGCTGGAA GATTCGATGG GAAGAGCTGG ATGGTGAAGA GAGTCAAAAG 
AAGAATGAAA AGAAGAAGGC GAAGAAAAGG AAAAATCATA ACGATTATCT ACCTGAATCT 
GATCCATTAT TAAGATCCAC ATCGAGGTCG TCAGTGAATT CGGACAAGAG ATCGTCCAGC 
TCGGGAACAA CTCGTAAAAT CTCAGCGATG ATTGATCGAA AGTTGAGCAT TTTCACGCGA 
AAAAAGAGCA CACCACCATC TGAATCACAA AAGAACGGAG GACTCACACC GAATTCGCTT 
CAAAAAGCGG AAAATGGAGA TTGTTCACCG ATTAATGAAG TTCAATTCAG ACTTCCATTA 
AATGATCGGA GAGTATCATC ACCATCATCA GAAGCGACGA GGAAAAAGAA TAGTAATGAA 
GAAGATCCGG AGAATGGTGC AAAGAAAAGT TTGAGCTTGA AAAATCGAAA ACTCAGCTTT 
GGAATGGTTT CATTCAAGAG TGGAAGTGGT GGATCAGTGG AAACAATTGC TCAAAATAAT 
ACGCAAATAT ACACGAAAAC GGCAATATTC AAGGGAGTTG TTGTGGCGAT CAAGAAGTTG 
AATATAGATC CAAAGAAGTA TCCACGTCTT GATTTATCAC GTGCTCAATT GATGGAATTA 
AAAAAGATGA AGGATCTTCA ACATGATCAT ATTACGAGAT TCACCGGAGC ATGTATCGAT 
TTTCCACATT ATTGTGTAGT AACTGAATAC TGTCCGAAAG GTTCATTGGA AGATATATTG 
GAAAATGAAA AAATCGAATT GGACAAACTG ATGAAGTACT CACTACTTCA CGATTTGGTT 
AAGGGTCTAT TCTTCCTGCA CAATAGTGAA ATTCGATCTC ACGGACGTCT GAAATCATCG 
AATTGTGTCG TTGACAGTCG ATTTGTGCTG AAAGTCACCG ATTTCGGACT TCACAGGCTT 
CATTGTTTAG AAGAAATTAA TTTGGAAGAA ATTGGAGAGC ACGCGTATTA TAAAAAAATG 
CTGTGGACGG CTCCAGAATT GCTCCGAGAT TCGAATGCTC CACCAATGGG AACCCAGAAG 
GGTGATATCT ATTCATTTGC AATTATTTTG CATGAAATGA TGTTCCGGAA GGGAGTTTTC 
GCATTGGAGA ATGAGGATTT ATCACCGAAT GAAATAGTGC AACGGGTACG AAAACCAGTA 
TCAGAAGATC AAGAACCACT TCGACCGTGG GTATCAGAAA CTGGTGAAGG TGAAGGTGAT 
GATGCATTAA ATGATACATT ATTATCATTA ATGGTTGCAT GTTGGTCTGA AGATCCACAT 
GAACGGCCTG AAGTATCATC TGTTCGAAAA GCGGTTCGAA GTTTGAATCG AGATAATGAA 
ACATCGAATC TTGTGGATAA TTTACTCAAA AGAATGGAAC AATATGCTAA TAATTTGGAA 
GGTCTTGTTG AGGAACGCAC GCAAGAATAT TTGGCGGAAA AGAAAAAGGT AGAAGAACTT 
CTTCATCAAC TTCTTCCACC TGCAATCGCC GACACTTTAA TTGCTGGACG AGCTGTTCAA 
GCTGAATCGT ATGATTGTGT AACTATCTAC TTTTCTGATA TTGTCGGATT CACATCACTA 
TCATCACAAT CAACACCAAT GCAAGTGGTC ACATTACTCA ATGATTTATA TTTGGCATTT 
GATGGAGTTG TAGACAATTT CAAAGTATAT AAAGTTGAGA CTATTGGTGA TGCATACATG 
GTAGTATCTG GATTACCAGA ACGACGAGAT GATCATGCTA ATCAAATAGC TCAAATGTCA 
CTATCATTAT TGCATAAAGT TAAGAATTTT GTGATTCGAC ATCGACCTCA TGAACAGCTC 
AAACTTCGTA TCGGAATGCA TTCAGGATCC GTTGTGGCAG GAGTCGTAGG TTCAAAAATG 
CCTCGATACT GTCTTTTCGG AGATACTGTG AACACTTCGA GTCGAATGGA AAGCAATGGA 
TTACCTCTCA AAATCCACGT GTCACAACAA ACGTACGATA TTCTGATGCA AGAAGCGGGC 
TTTAAACTCG AACTACGAGG TTCAGTTGAG ATGAAAGGCA AAGGAATGCA GACTACTTAT 
TGGTTACGAG GTTACAAAGA CGTTGAGATT CCCGATTTTG GCGAAGAATT TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcy-28 ( NM_001262699.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001249628.1 Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

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CloneID	OCa337137
Clone ID Related Accession (Same CDS sequence)	NM_001262699.2 , NM_001262699.1
Accession Version	NM_001262699.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Receptor-type guanylate cyclase gcy-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). COMPLETENESS: incomplete on both ends.

ATGTGGATTA ACAGCACGCG AACACTTATA TTTAATCATT TCGTACGCAT TGTACTGGTG 
CTTCTTCTGT CTCACGAGGT CGTCACAAAG TCTAATAGAA AATTTGAGGA GATCTCTGTC 
AAGCATCCGA TCCATTTGGT CATTCCATTG CCAGACGATG ATGATTTTAG TGATGGAAAA 
AATCCATTTT TGTTCTCATC TCACAAAGTA AAACCGCTCG CTGATTTGGC TCTGGAGAGA 
GTTTATCGAG AAGGAATCCT ACCCAACAAC TCTGTAAATC TAATTTATCG AGACTCAAAA 
CTATCGGATG CTATTGGTCC AAACGTGGCA GTTGAGCAAC TTCTTCGAAA ACAAATTGAT 
TGTATAATTG GTTATGCGTA TGGATATGCA CTTGCACCAG TTGCCAGAAT GTCTCCATAT 
TGGAAAAATG GAATTCCGAT AATCACACCA ATTGGCTTGA CAATGAGTTT AGATGATAAA 
CGAGAATATC AGTTAATGAC AAGAATTAAT AGTCCGTATA AAGTAGTCTC CAGCGCTGTA 
TCAACACTAT TCAACACATA TAAATGGAAA CGCCACATTT TTATGTTTCA CCACGCAAAA 
GCGCCGTCTG TGGCCGTTGG AGAATGCTTC CTTCTGATGG CAAGTCTACA GCATCCACTT 
CGAAAGGTCA TGGAAATGCA ACACAACTTC TTCACGTTCA ACGAAGATCA CGGAGCGAAT 
ATATCAAAAG CGGAACGGCA CAATCAATTT CGGAACTATT TGCGAGCGAG CAGTAATATG 
GCGAATGTGA TTATCCTATG TGCATCACCG GATACTGTAC GTGAAATAAT GTTGGCTGCT 
CATGATCTTG GAATGGCGAC AAGTGGTGAA TATGTATTCA TTAATATCGA TGTTTCAACG 
GGATCACACG CCGAACAACC ATGGATTCGA GCAAATGACA CGAATAATGA GGAGAACGAG 
AAAGCAAAAG AAGCATATCG AGCATTGAAA ACAATTTCAT TGAGACGATC CGATTTGGAT 
GAATATAAGA ATTTTGAACT TCGAGTCAAA GAACGAGCAG ATCAAAAATA TAATTATACG 
AATATCACCG GAAAAGATTA TGAGATGAAC AATTTCATCA GTGCATTTTA CGATGCGGTT 
CTTCTCTATG CCATTGCTCT AAATGAGACA ATTCAATCAG GTCTTGATCC ACGAAATGGA 
CATAATATCA CGTCACGAAT GTGGGGACGA ACATTTGTCG GAATTACTGG AAATGTATCG 
ATTGATCACA ATGGTGATAG GTATTCGGAT TATTCTCTAC TCGATTTGGA TCCCGTTCAG 
AATAGATTTG TGGAAGTAGC CTACTACAGT GGAGCATCAA ATCAACTAAA AACTGTGGGA 
CAACTGCATT GGGTTGGCGG AAAACCTCCA ACAGATCTAC CTATATGTGG TTACGATAAG 
AGCAAGTGTC CGCAGGGTTA TCCACTTCAT GTCTATCTTC TAATGGGTTC ATTCCTCCTA 
ATTCTCGTCC TTGTCGGTCT ATTCATCTTC TTCTGGCGTC GCTACAAACT TGAACAAGAG 
CTCGCCGCGA TGAGCTGGAA GATTCGATGG GAAGAGCTGG ATGGTGAAGA GAGTCAAAAG 
AAGAATGAAA AGAAGAAGGC GAAGAAAAGG AAAAATCATA ACGATTATCT ACCTGAATCT 
GATCCATTAT TAAGATCCAC ATCGAGGTCG TCAGTGAATT CGGACAAGAG ATCGTCCAGC 
TCGGGAACAA CTCGTAAAAT CTCAGCGATG ATTGATCGAA AGTTGAGCAT TTTCACGCGA 
AAAAAGAGCA CACCACCATC TGAATCACAA AAGAACGGAG GACTCACACC GAATTCGCTT 
CAAAAAGCGG AAAATGGAGA TTGTTCACCG ATTAATGAAG TTCAATTCAG ACTTCCATTA 
AATGATCGGA GAGTATCATC ACCATCATCA GAAGCGACGA GGAAAAAGAA TAGTAATGAA 
GAAGATCCGG AGAATGGTGC AAAGAAAAGT TTGAGCTTGA AAAATCGAAA ACTCAGCTTT 
GGAATGGTTT CATTCAAGAG TGGAAGTGGT GGATCAGTGG AAACAATTGC TCAAAATAAT 
ACGCAAATAT ACACGAAAAC GGCAATATTC AAGGGAGTTG TTGTGGCGAT CAAGAAGTTG 
AATATAGATC CAAAGAAGTA TCCACGTCTT GATTTATCAC GTGCTCAATT GATGGAATTA 
AAAAAGATGA AGGATCTTCA ACATGATCAT ATTACGAGAT TCACCGGAGC ATGTATCGAT 
TTTCCACATT ATTGTGTAGT AACTGAATAC TGTCCGAAAG GTTCATTGGA AGATATATTG 
GAAAATGAAA AAATCGAATT GGACAAACTG ATGAAGTACT CACTACTTCA CGATTTGGTT 
AAGGGTCTAT TCTTCCTGCA CAATAGTGAA ATTCGATCTC ACGGACGTCT GAAATCATCG 
AATTGTGTCG TTGACAGTCG ATTTGTGCTG AAAGTCACCG ATTTCGGACT TCACAGGCTT 
CATTGTTTAG AAGAAATTAA TTTGGAAGAA ATTGGAGAGC ACGCGTATTA TAAAAAAATG 
CTGTGGACGG CTCCAGAATT GCTCCGAGAT TCGAATGCTC CACCAATGGG AACCCAGAAG 
GGTGATATCT ATTCATTTGC AATTATTTTG CATGAAATGA TGTTCCGGAA GGGAGTTTTC 
GCATTGGAGA ATGAGGATTT ATCACCGAAT GAAATAGTGC AACGGGTACG AAAACCAGTA 
TCAGAAGATC AAGAACCACT TCGACCGTGG GTATCAGAAA CTGGTGAAGG TGAAGGTGAT 
GATGCATTAA ATGATACATT ATTATCATTA ATGGTTGCAT GTTGGTCTGA AGATCCACAT 
GAACGGCCTG AAGTATCATC TGTTCGAAAA GCGGTTCGAA GTTTGAATCG AGATAATGAA 
ACATCGAATC TTGTGGATAA TTTACTCAAA AGAATGGAAC AATATGCTAA TAATTTGGAA 
GGTCTTGTTG AGGAACGCAC GCAAGAATAT TTGGCGGAAA AGAAAAAGGT AGAAGAACTT 
CTTCATCAAC TTCTTCCACC TGCAATCGCC GACACTTTAA TTGCTGGACG AGCTGTTCAA 
GCTGAATCGT ATGATTGTGT AACTATCTAC TTTTCTGATA TTGTCGGATT CACATCACTA 
TCATCACAAT CAACACCAAT GCAAGTGGTC ACATTACTCA ATGATTTATA TTTGGCATTT 
GATGGAGTTG TAGACAATTT CAAAGTATAT AAAGTTGAGA CTATTGGTGA TGCATACATG 
GTAGTATCTG GATTACCAGA ACGACGAGAT GATCATGCTA ATCAAATAGC TCAAATGTCA 
CTATCATTAT TGCATAAAGT TAAGAATTTT GTGATTCGAC ATCGACCTCA TGAACAGCTC 
AAACTTCGTA TCGGAATGCA TTCAGGATCC GTTGTGGCAG GAGTCGTAGG TTCAAAAATG 
CCTCGATACT GTCTTTTCGG AGATACTGTG AACACTTCGA GTCGAATGGA AAGCAATGGA 
TTACCTCTCA AAATCCACGT GTCACAACAA ACGTACGATA TTCTGATGCA AGAAGCGGGC 
TTTAAACTCG AACTACGAGG TTCAGTTGAG ATGAAAGGCA AAGGAATGCA GACTACTTAT 
TGGTTACGAG GTTACAAAGA CGTTGAGATT CCCGATTTTG GCGAAGAATT TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001249628.1
CDS	1..3717
Translation	MWINSTRTLIFNHFVRIVLVLLLSHEVVTKSNRKFEEISVKHPIHLVIPLPDDDDFSDGKNPFLFSSHKVKPLADLALERVYREGILPNNSVNLIYRDSKLSDAIGPNVAVEQLLRKQIDCIIGYAYGYALAPVARMSPYWKNGIPIITPIGLTMSLDDKREYQLMTRINSPYKVVSSAVSTLFNTYKWKRHIFMFHHAKAPSVAVGECFLLMASLQHPLRKVMEMQHNFFTFNEDHGANISKAERHNQFRNYLRASSNMANVIILCASPDTVREIMLAAHDLGMATSGEYVFINIDVSTGSHAEQPWIRANDTNNEENEKAKEAYRALKTISLRRSDLDEYKNFELRVKERADQKYNYTNITGKDYEMNNFISAFYDAVLLYAIALNETIQSGLDPRNGHNITSRMWGRTFVGITGNVSIDHNGDRYSDYSLLDLDPVQNRFVEVAYYSGASNQLKTVGQLHWVGGKPPTDLPICGYDKSKCPQGYPLHVYLLMGSFLLILVLVGLFIFFWRRYKLEQELAAMSWKIRWEELDGEESQKKNEKKKAKKRKNHNDYLPESDPLLRSTSRSSVNSDKRSSSSGTTRKISAMIDRKLSIFTRKKSTPPSESQKNGGLTPNSLQKAENGDCSPINEVQFRLPLNDRRVSSPSSEATRKKNSNEEDPENGAKKSLSLKNRKLSFGMVSFKSGSGGSVETIAQNNTQIYTKTAIFKGVVVAIKKLNIDPKKYPRLDLSRAQLMELKKMKDLQHDHITRFTGACIDFPHYCVVTEYCPKGSLEDILENEKIELDKLMKYSLLHDLVKGLFFLHNSEIRSHGRLKSSNCVVDSRFVLKVTDFGLHRLHCLEEINLEEIGEHAYYKKMLWTAPELLRDSNAPPMGTQKGDIYSFAIILHEMMFRKGVFALENEDLSPNEIVQRVRKPVSEDQEPLRPWVSETGEGEGDDALNDTLLSLMVACWSEDPHERPEVSSVRKAVRSLNRDNETSNLVDNLLKRMEQYANNLEGLVEERTQEYLAEKKKVEELLHQLLPPAIADTLIAGRAVQAESYDCVTIYFSDIVGFTSLSSQSTPMQVVTLLNDLYLAFDGVVDNFKVYKVETIGDAYMVVSGLPERRDDHANQIAQMSLSLLHKVKNFVIRHRPHEQLKLRIGMHSGSVVAGVVGSKMPRYCLFGDTVNTSSRMESNGLPLKIHVSQQTYDILMQEAGFKLELRGSVEMKGKGMQTTYWLRGYKDVEIPDFGEEFA

Target ORF information:

RefSeq Version	NM_001262699.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Receptor-type guanylate cyclase gcy-28 (gcy-28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001262699.1

ORF Insert Sequence:

ATGTGGATTA ACAGCACGCG AACACTTATA TTTAATCATT TCGTACGCAT TGTACTGGTG 
CTTCTTCTGT CTCACGAGGT CGTCACAAAG TCTAATAGAA AATTTGAGGA GATCTCTGTC 
AAGCATCCGA TCCATTTGGT CATTCCATTG CCAGACGATG ATGATTTTAG TGATGGAAAA 
AATCCATTTT TGTTCTCATC TCACAAAGTA AAACCGCTCG CTGATTTGGC TCTGGAGAGA 
GTTTATCGAG AAGGAATCCT ACCCAACAAC TCTGTAAATC TAATTTATCG AGACTCAAAA 
CTATCGGATG CTATTGGTCC AAACGTGGCA GTTGAGCAAC TTCTTCGAAA ACAAATTGAT 
TGTATAATTG GTTATGCGTA TGGATATGCA CTTGCACCAG TTGCCAGAAT GTCTCCATAT 
TGGAAAAATG GAATTCCGAT AATCACACCA ATTGGCTTGA CAATGAGTTT AGATGATAAA 
CGAGAATATC AGTTAATGAC AAGAATTAAT AGTCCGTATA AAGTAGTCTC CAGCGCTGTA 
TCAACACTAT TCAACACATA TAAATGGAAA CGCCACATTT TTATGTTTCA CCACGCAAAA 
GCGCCGTCTG TGGCCGTTGG AGAATGCTTC CTTCTGATGG CAAGTCTACA GCATCCACTT 
CGAAAGGTCA TGGAAATGCA ACACAACTTC TTCACGTTCA ACGAAGATCA CGGAGCGAAT 
ATATCAAAAG CGGAACGGCA CAATCAATTT CGGAACTATT TGCGAGCGAG CAGTAATATG 
GCGAATGTGA TTATCCTATG TGCATCACCG GATACTGTAC GTGAAATAAT GTTGGCTGCT 
CATGATCTTG GAATGGCGAC AAGTGGTGAA TATGTATTCA TTAATATCGA TGTTTCAACG 
GGATCACACG CCGAACAACC ATGGATTCGA GCAAATGACA CGAATAATGA GGAGAACGAG 
AAAGCAAAAG AAGCATATCG AGCATTGAAA ACAATTTCAT TGAGACGATC CGATTTGGAT 
GAATATAAGA ATTTTGAACT TCGAGTCAAA GAACGAGCAG ATCAAAAATA TAATTATACG 
AATATCACCG GAAAAGATTA TGAGATGAAC AATTTCATCA GTGCATTTTA CGATGCGGTT 
CTTCTCTATG CCATTGCTCT AAATGAGACA ATTCAATCAG GTCTTGATCC ACGAAATGGA 
CATAATATCA CGTCACGAAT GTGGGGACGA ACATTTGTCG GAATTACTGG AAATGTATCG 
ATTGATCACA ATGGTGATAG GTATTCGGAT TATTCTCTAC TCGATTTGGA TCCCGTTCAG 
AATAGATTTG TGGAAGTAGC CTACTACAGT GGAGCATCAA ATCAACTAAA AACTGTGGGA 
CAACTGCATT GGGTTGGCGG AAAACCTCCA ACAGATCTAC CTATATGTGG TTACGATAAG 
AGCAAGTGTC CGCAGGGTTA TCCACTTCAT GTCTATCTTC TAATGGGTTC ATTCCTCCTA 
ATTCTCGTCC TTGTCGGTCT ATTCATCTTC TTCTGGCGTC GCTACAAACT TGAACAAGAG 
CTCGCCGCGA TGAGCTGGAA GATTCGATGG GAAGAGCTGG ATGGTGAAGA GAGTCAAAAG 
AAGAATGAAA AGAAGAAGGC GAAGAAAAGG AAAAATCATA ACGATTATCT ACCTGAATCT 
GATCCATTAT TAAGATCCAC ATCGAGGTCG TCAGTGAATT CGGACAAGAG ATCGTCCAGC 
TCGGGAACAA CTCGTAAAAT CTCAGCGATG ATTGATCGAA AGTTGAGCAT TTTCACGCGA 
AAAAAGAGCA CACCACCATC TGAATCACAA AAGAACGGAG GACTCACACC GAATTCGCTT 
CAAAAAGCGG AAAATGGAGA TTGTTCACCG ATTAATGAAG TTCAATTCAG ACTTCCATTA 
AATGATCGGA GAGTATCATC ACCATCATCA GAAGCGACGA GGAAAAAGAA TAGTAATGAA 
GAAGATCCGG AGAATGGTGC AAAGAAAAGT TTGAGCTTGA AAAATCGAAA ACTCAGCTTT 
GGAATGGTTT CATTCAAGAG TGGAAGTGGT GGATCAGTGG AAACAATTGC TCAAAATAAT 
ACGCAAATAT ACACGAAAAC GGCAATATTC AAGGGAGTTG TTGTGGCGAT CAAGAAGTTG 
AATATAGATC CAAAGAAGTA TCCACGTCTT GATTTATCAC GTGCTCAATT GATGGAATTA 
AAAAAGATGA AGGATCTTCA ACATGATCAT ATTACGAGAT TCACCGGAGC ATGTATCGAT 
TTTCCACATT ATTGTGTAGT AACTGAATAC TGTCCGAAAG GTTCATTGGA AGATATATTG 
GAAAATGAAA AAATCGAATT GGACAAACTG ATGAAGTACT CACTACTTCA CGATTTGGTT 
AAGGGTCTAT TCTTCCTGCA CAATAGTGAA ATTCGATCTC ACGGACGTCT GAAATCATCG 
AATTGTGTCG TTGACAGTCG ATTTGTGCTG AAAGTCACCG ATTTCGGACT TCACAGGCTT 
CATTGTTTAG AAGAAATTAA TTTGGAAGAA ATTGGAGAGC ACGCGTATTA TAAAAAAATG 
CTGTGGACGG CTCCAGAATT GCTCCGAGAT TCGAATGCTC CACCAATGGG AACCCAGAAG 
GGTGATATCT ATTCATTTGC AATTATTTTG CATGAAATGA TGTTCCGGAA GGGAGTTTTC 
GCATTGGAGA ATGAGGATTT ATCACCGAAT GAAATAGTGC AACGGGTACG AAAACCAGTA 
TCAGAAGATC AAGAACCACT TCGACCGTGG GTATCAGAAA CTGGTGAAGG TGAAGGTGAT 
GATGCATTAA ATGATACATT ATTATCATTA ATGGTTGCAT GTTGGTCTGA AGATCCACAT 
GAACGGCCTG AAGTATCATC TGTTCGAAAA GCGGTTCGAA GTTTGAATCG AGATAATGAA 
ACATCGAATC TTGTGGATAA TTTACTCAAA AGAATGGAAC AATATGCTAA TAATTTGGAA 
GGTCTTGTTG AGGAACGCAC GCAAGAATAT TTGGCGGAAA AGAAAAAGGT AGAAGAACTT 
CTTCATCAAC TTCTTCCACC TGCAATCGCC GACACTTTAA TTGCTGGACG AGCTGTTCAA 
GCTGAATCGT ATGATTGTGT AACTATCTAC TTTTCTGATA TTGTCGGATT CACATCACTA 
TCATCACAAT CAACACCAAT GCAAGTGGTC ACATTACTCA ATGATTTATA TTTGGCATTT 
GATGGAGTTG TAGACAATTT CAAAGTATAT AAAGTTGAGA CTATTGGTGA TGCATACATG 
GTAGTATCTG GATTACCAGA ACGACGAGAT GATCATGCTA ATCAAATAGC TCAAATGTCA 
CTATCATTAT TGCATAAAGT TAAGAATTTT GTGATTCGAC ATCGACCTCA TGAACAGCTC 
AAACTTCGTA TCGGAATGCA TTCAGGATCC GTTGTGGCAG GAGTCGTAGG TTCAAAAATG 
CCTCGATACT GTCTTTTCGG AGATACTGTG AACACTTCGA GTCGAATGGA AAGCAATGGA 
TTACCTCTCA AAATCCACGT GTCACAACAA ACGTACGATA TTCTGATGCA AGAAGCGGGC 
TTTAAACTCG AACTACGAGG TTCAGTTGAG ATGAAAGGCA AAGGAATGCA GACTACTTAT 
TGGTTACGAG GTTACAAAGA CGTTGAGATT CCCGATTTTG GCGAAGAATT TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gcy-28 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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