ubr-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr-1
Entrez Gene ID	171953
Full Name	E3 ubiquitin-protein ligase ubr-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_058830.5	NP_491231.2	E3 ubiquitin-protein ligase ubr-1
NM_058830.4	NP_491231.2	E3 ubiquitin-protein ligase ubr-1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that CED-3 caspase and the E3 ubiquitin ligase UBR-1 form a complex that couples their in vivo activities, allowing for recognition and rapid degradation of LIN-28
Title: Coupled Caspase and N-End Rule Ligase Activities Allow Recognition and Degradation of Pluripotency Factor LIN-28 during Non-Apoptotic Development.

The following ubr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336635	NM_058830.5 Latest version!	Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa336635	NM_058830.4	Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr-1 ( NM_058830.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491231.2 Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), mRNA.

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CloneID	OCa336635
Clone ID Related Accession (Same CDS sequence)	NM_058830.5 , NM_058830.4
Accession Version	NM_058830.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase ubr-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058830.4.

ATGATTGTCG ATTTAATACA ATCAGCTCGA CAGGGTGAAT GGGCCCAAGT ACGCCAGCTG 
CTGCTCAAGC ACTGGCTTGT ACAGGTCCCA GAGGTGTTCG AAGTGAATTC GGATTTGCCA 
TGGGACAATA CAGCCGCGAA TGAACGAATT CTAGGTTCCC AAGGAGAGAT TCTCCTAGCC 
CCACTGGTCA GTGCATTTGT ACTAGACGTT CGGAACACAA AGAGCACACT GGAAGCAATG 
AATGGCATTG CCGGAGTTGA TCCTGCAAGA CGGGGACAAA TTTGTGGCCA TGTATTTAAA 
AACGGAGAAC TTACGTATAC ATGTCTGGAT TGTGCCACTG ATGGGACATG TGTCATGTGT 
CTTCAGTGTT TTGAAGTTTC AATTCATAAA TCTCATAAAT ACAAAATGCA CTCTTCCTCC 
GGTTCGGGAT ATTGTGACTG TGGTGATGCA GATGCATGGA CAGAAGGTTA CGCATGTGCA 
AATCACGAGA AAAAGGACGA TGAAGAGGCG GCAGTGTTGG CTCCAGAATT GAAAAAACGA 
TGTGAACAGC TGGTGGAAAT CATTTTACAG TTTTCCCTTT CAATGATCAC TCATAAAGAT 
GATCTCAAAC TTCCCGAAAT TTTCGAGAAA ATGAAGCCAG AGGTTACAAA TGAGGCACAG 
CAATATTTGA CGGTTCTTTA CAATGATGAA ACTCACACTT ATGAATCTGT GATCAAAGTT 
CTGGAATTGT ACATACATTG TACAAAGGAT CAGGCGATGC TTGTAGCCAC AATTGTCGAT 
CGAGAAGGTC GAAGTGCTGT GAAACTTGGC AGTAAGGCTG ATTGCACCAA AGCTAAGGAT 
GATGTTCAGA GAAAAACAGC TCGAGATCCA ACGTCAATTC GAAGATCATC GAATCATAAT 
CTACCGTTAA GTGTTAAGGT TATGGATACC ACGCTTTTCG CGCTTCAGAA TTTTTCGATC 
TCCCTTCTTA CTTGGCTCAA CACACAGATG GATGTTTTTC CTCCCCTTCG CGAAATTGTT 
GGAGAAATTC TGCTGAGTTC AAAGTTTGCA CTGAAAAAGA ATTACACGCG GAAAATGAAG 
TCAGAGGATC AACGTCTAGT TGCCGGAATT ATCAGAAATG TTATGGTTTT GCCGGATGAT 
GAAGAGGAAG AACTATTCGC ACTTGATGGA AGAATGGATG TTGATGAAAT GGATGACGAC 
GATATTGGTG GAGAAGCTCT GCAAATGGAA ATTGACGCGG ATGATGAAGA AGAGATAACA 
GCAGCGTTGG CAGGAGTTTC AGAACATCAA GAATCTCCAG GTCCTTCACG AGATTCTTCA 
ACATTCACAA TGCTAGAGAA TATTCTTCTC CAGGATACAC AAATGTGGAA AGCTGGAAGA 
TCGATTTTGC ATCAAATGCT CATGCGGACA GTTTTCATGA TTTATGATCA GAAAGTTCGA 
TTTGCAAAGG CTTTTATGCT ACATTATAAT GAAATTTACG AGGATTTCAT TAAAGATGAT 
CATGAGATGG ATGTTTCAGT TGTTGGGTTA TCAGTTCAAT TCATGACTGT TCCTAGTCTG 
GCTCGGAAAC TTGTCGCCGA AGATCAAGCA TTCAGTGTGA TATCGAAAGC GATTCGGGAT 
CAGACTGATA AATTTGTGAA GTATTACAAT GACGGAAAAA TCGCGAGATT TGATTTCACA 
AGTCGCTCAT TTCCACCAGA ATTAAGAAGA TCCCTTCATA TCACTCGAGA TATGGCCTAT 
ATCCTGAATG CTGTGCCATC GGAAAGCGAC TGGAATCGAG AACTTATCGA TGGATTTGTT 
CAAGGATTCG CTGATTTTCT GCTTTTCCTG CAGCATCTGC AAGGAATGGA TGAGGTGAAA 
CGACAAGCCG TTGAGCATCA AGTGTGGGAA TCTGAGTGGG AAACAGCGTT CAATATCCTT 
TTACGATTGA AAGACGCGAT TTCGATGATT ATTGGATGGG CAGAAACTAA TGAAGAAGTT 
CATAATCGTC TAATGATAAT GTGCCTTGAA TTGATGAATC GTATGCCACC TGTCTATACA 
AAATCCGAGG AGGATACCTA TGAATTGACG GTCACTATCA ACGGAGAATC ATGTCGAATA 
TCACATTTTG ACGTGCTTAA ATCATCCACC TCGGTCCATC AGCCGGTTGT TCGAATTATC 
GCGGGTCTCT TTAGTGCTTC GAATTACACG GGATTTTTGT TGAATAGAAG TAATAATTCA 
CATACGAGTT TGAATCAAGA AAGAATCAAA GAACTTATCA ACTGTAAGGA CGAGACGAAT 
CTCTACGAAC TGAGTCTCCG AGTGCTCGTC CTGTGTGCAC AATCCAACGC GACACTTTGG 
AGAAGAAATG GATTTTCGCT GATAAACCAG ATTCATAATT ATTTCTCTCC ACTCTGTCGA 
AATGAGATGT TCGATCGTGA TGTTCTGATG ATGCAAGTAG GAGCTGCACT CACACCGTCA 
ACAAAATTCA TTTTCCACCT TCTTCATCGA TTCCGTCTCT TTAAATGGGC TACATCCGAG 
TTTGAACAAG ACAAGGCGAA TGAGAAACCA GCGAAACCGG AATCAGAGGA TCTGAGTAAG 
ACGTTGGTGA TGATCGCTGA AGAGTTCATA CAATGTCTGA TTCTGATTCT CTGTGAACGG 
TATACATACG GAGTTGGAAA GACTCGTCCG ATGGATCAGA TGAAACGAGA AGTTATCCAT 
ATTCTTTGTA CTGGATCTCA TACGTTCAGT CATATTCAAC AGAAAATGTC ACATGATATC 
AATTCGAAGC GTCTTTCTCT TCATGAAGCA GTAAATCTGG TTGCAGACTT CCGAAAGCCA 
CTGGCCACCA CAGCTGGACA ATTCCATTGC AAAGAGTCGA GCCTTCCAAC CTATTCACCA 
TTCTTCATGC ATTATTCAAA AAGTGATCAG TCAGCTGCTG AACAGAGTCA GGCAAGAGTT 
CGTGCGAAAA TGGAAAAGTC GGTTCGAGCT TGTGCGCCGC CTATTCTTCC GGATTTTCAA 
ACATTTTTCG AGCGGATTCC CGAAATTCTT ACCACAAATG TTCTTATTCA CGTTGTTCGT 
CTGATTATCG ATAGAACAGC TCGACGTAGC CGTTTCTCAA GTGATCGTCT CTTCCACAAA 
ACACTATATC TGATCGGAAT CGCGTTAAAC GAAGAGGAAA AGAATCCATC TTTCGGATTT 
ACACAACGTG CAGAGGAATC GATCGGTCTT TTGTCGCTTT TAGAAGGACT TGTTGGAAAA 
CCAGAGTCAT CGATTTGTCC GATCCTTCTC GAAGTGACCG TCGAGAAATA CAGAAAATTG 
ATTAAAGCTA GAGCAGGTGT TCCTGAAGCA GCTCCAGCTC CTGAAAACAA GCCAGCACAA 
TCCGAAGAAG AAATCAAAGC GAAGCGAGCT GCAAGGGCGG CGGAAATGAG ACAAAAAGCG 
ATGGCAAAAA TGTCGAATAT GCAGTCAAAG TTTATGAAGA AAATTGAGGA CGAAGAGAAG 
AAAGACGAAT CACAGACACC TTCTGAAAAA TCTGAAACTG TTGTGAAGAA GGACGACTAT 
GATAATAAGC ATTTCTTCGA TGAAGATGTT GTGAAGCAAG TTGGTCACGA TTTTCCGGTT 
TGTATCGGTG CAAATAAGTG GCACGCGGAG CTCGTGAAAC CCCGAACATT GACGTGTATT 
CTGTGTCAAG AGGATGAAAT AATTGCACCG CAACAGGGAA AACCAATGGT TTGCGCAGCG 
TTTATTCAAC AATCTCAACT TTTCACTCAT AAGAACAAAA ATGGAGAGCT CATGACGGCT 
TCAAGTGGGA CAATATCTAC ACGTGATCTT CTAACAGCAC CAGCTACACT TCAATATGGA 
GTTGATGTTT CCACGTGTTC ACATAGTATG CATTATGAGT GTTATCGAAG TCTCGCCGAA 
GCGAATCGAT CTCGCGAGAG TTTGAGAGCC CGTCAAGTTG GTCAACATTC GCATAAAATG 
GTTGATACCG AGAATGGAGA ATATCAGTGT CCGTTGTGCA AACGATTGTC GAATGCGGCG 
ATTCCAGTAT TGCCAGCCTA TCAGCTGACG AATCAAAATG GCTTCTCCAC TGTCTCTGGA 
GCCGGAAAGG AGAACTTTGA CACATGGGTG GCACGAGTCA AACGGAATCT GGAAATGCCG 
CTCAGTTCGG AATCTGTCAG CAAGAAGGGT CATAGTAGGA AGAGATCACA TTCCGAACGA 
TCACTGCTGG ATCTTGAGAA GCTCAGCAAG GATCCGGATA CTGCCAACAC GTCTGCTGGT 
GTATTACAAT TTGGAGCCAT GGAAATGAGT AGTGCTACAC ATATGCCAGC GTCTGCCGAA 
TCTCAAATGC TTATGACAAC GTCACCTTCT CAAGATGACG TAGAATTCTA TAATGAATTG 
GCTGCGATGT TTGTGGATCA AGATGTGAAT AATACTACAT CACCGGCAGC TACACCTGAA 
ACAATCCCAG CTATAGGTTC ATCGTCTCGA ATTCCTGAAT CTCAAGAAAG TGGAAAAAAG 
CCGTTGAGTA GTCAAATCCA GCATGTACTC TATTCACTAA TTCGACCATT CCCCGCGTTG 
ATCAACTCGA ATCGTATATG TTCTTCTTCT TTTGAAGGTT TTGAGGAACC AATAAAAGAT 
CTTGGGAAGA ATATGATGAA GTTCCGGAAA CGTGGCAATG AGCTGAAGAC GAATTTCATA 
GAAAAACATC TGAAGGGGTA CGTGATTTCG ACAGTTACCT GGCAAAGTAC TGCTCACGTG 
GCTCGAGCCA TTTCATCGTA CCTCCACTAC GACAAAAAAC CACTTTTTGG AGCATTAAAC 
ACTCGTCAAA GAGATTGTCT TTCTGCAATG GCTCGTCTAT GTGCATCACT ATCACATAAT 
ATGCAGTTCT TGCTTCACGC AGTCTCCGAC ATGCTTCGAG TATTCCTTTG TGAACCACCG 
AGACCGAAAT TAGCTCAAAC ACCGGGATCT CCGTTGCTAA GTGCTCCGTC GACATCTTCG 
TTCACACCTG CACCTGCTCA AATTCCGCAT TCGGGTACAA ACTTTGCATT TTTGGTGCAG 
TTATTCAATC CAGCTGGTCC GAGGAAGAAT GTGAATCTGA ACATTTTGCA AATCGATATT 
TTGAGTTTGG CGATTTCTCT GATGATGACA ATCGGATGGA CGTGGAACAA CGGAACACAA 
TCCATGAACT CCTCGTCAAC GCATCAAAAA GCTCGATTAC TGACACCTGA TGGATCTGTA 
GATGAGGCAT ATGTTCTTCG TTTGTCACTT TTGGCACATT ATTTCCAAAT CATCGCAACA 
CACTCAGAAG CGGACGGAAA TGATGTTAAT ATGGAAGAGG AACAGGAATC ACAGATGGAA 
GTGGATCCGG TTGCTGCTCA GACGATCAGG AAGCTTTACG CTCTTTGTCA TCCATTTGAT 
GGGCCATTGC GACGAGTTGA TATTCTTTGG AGGAAGATGG AAGAAGGTGC TCAATCTCTA 
CTTCGTCCGA TTGCTCTTTT GTATCATTTC ATCACACTTG TCGATCCACC AGAGGCTCTG 
AAAGATCCGT CAATCAATAG TTCGGAGCCA TTATTCCGCT ATCTGGGTTT ACCGCATAAG 
ATTGAAGAAC AAATCAGTGG AAGTATGCTC GAAAAGTTGT TCACAATGTG GTCATCATCG 
ATTCCAAGTG ATCAAGCTCT TCGACAGGAT CTTGTTGTTC AACCAGTTCG ACCGAATCTT 
CTTGTTGAAT TGCCAGAGAA GTATAGTCAA TTAATAAATC AAGTTGCCAC ATTCAAATGT 
CCAACGATAC CGATTGAAGA GTCAACGTCA AATGTTCCGA CACTATGTCT AGTGTGCGGA 
ACTATCCTCT GCTCACAGGC ATATTGCTGT CAGAAAATTA TCAACAAACA ATCATATGGA 
GCATGTCGTT ATCATATGTC ACAGTGTTCC GGATCAGTGG GAATGTTTCT ACGAGTTCGA 
GACTGTAGCC TTGTATTGAT GACGACACGA AAACGCGGTT GCTTCCGTCC AGCACCATAT 
GTCGACGAAT TCGGAGAGGT TGATCAAGGA TTCCGACGTG GAAATCCCCT TCACCTGAAC 
CCTGAACTCT ATCAGAAGCT GAAGTCTTTG TGGCTTCAGC AGGGTATCAC TGAAGAAGTT 
GTGAACTACA ATGAAATTGA CTTCCGCAAT GTGCAATACG ATTGGGGTCA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491231.2
CDS	14..6190
Translation	MIVDLIQSARQGEWAQVRQLLLKHWLVQVPEVFEVNSDLPWDNTAANERILGSQGEILLAPLVSAFVLDVRNTKSTLEAMNGIAGVDPARRGQICGHVFKNGELTYTCLDCATDGTCVMCLQCFEVSIHKSHKYKMHSSSGSGYCDCGDADAWTEGYACANHEKKDDEEAAVLAPELKKRCEQLVEIILQFSLSMITHKDDLKLPEIFEKMKPEVTNEAQQYLTVLYNDETHTYESVIKVLELYIHCTKDQAMLVATIVDREGRSAVKLGSKADCTKAKDDVQRKTARDPTSIRRSSNHNLPLSVKVMDTTLFALQNFSISLLTWLNTQMDVFPPLREIVGEILLSSKFALKKNYTRKMKSEDQRLVAGIIRNVMVLPDDEEEELFALDGRMDVDEMDDDDIGGEALQMEIDADDEEEITAALAGVSEHQESPGPSRDSSTFTMLENILLQDTQMWKAGRSILHQMLMRTVFMIYDQKVRFAKAFMLHYNEIYEDFIKDDHEMDVSVVGLSVQFMTVPSLARKLVAEDQAFSVISKAIRDQTDKFVKYYNDGKIARFDFTSRSFPPELRRSLHITRDMAYILNAVPSESDWNRELIDGFVQGFADFLLFLQHLQGMDEVKRQAVEHQVWESEWETAFNILLRLKDAISMIIGWAETNEEVHNRLMIMCLELMNRMPPVYTKSEEDTYELTVTINGESCRISHFDVLKSSTSVHQPVVRIIAGLFSASNYTGFLLNRSNNSHTSLNQERIKELINCKDETNLYELSLRVLVLCAQSNATLWRRNGFSLINQIHNYFSPLCRNEMFDRDVLMMQVGAALTPSTKFIFHLLHRFRLFKWATSEFEQDKANEKPAKPESEDLSKTLVMIAEEFIQCLILILCERYTYGVGKTRPMDQMKREVIHILCTGSHTFSHIQQKMSHDINSKRLSLHEAVNLVADFRKPLATTAGQFHCKESSLPTYSPFFMHYSKSDQSAAEQSQARVRAKMEKSVRACAPPILPDFQTFFERIPEILTTNVLIHVVRLIIDRTARRSRFSSDRLFHKTLYLIGIALNEEEKNPSFGFTQRAEESIGLLSLLEGLVGKPESSICPILLEVTVEKYRKLIKARAGVPEAAPAPENKPAQSEEEIKAKRAARAAEMRQKAMAKMSNMQSKFMKKIEDEEKKDESQTPSEKSETVVKKDDYDNKHFFDEDVVKQVGHDFPVCIGANKWHAELVKPRTLTCILCQEDEIIAPQQGKPMVCAAFIQQSQLFTHKNKNGELMTASSGTISTRDLLTAPATLQYGVDVSTCSHSMHYECYRSLAEANRSRESLRARQVGQHSHKMVDTENGEYQCPLCKRLSNAAIPVLPAYQLTNQNGFSTVSGAGKENFDTWVARVKRNLEMPLSSESVSKKGHSRKRSHSERSLLDLEKLSKDPDTANTSAGVLQFGAMEMSSATHMPASAESQMLMTTSPSQDDVEFYNELAAMFVDQDVNNTTSPAATPETIPAIGSSSRIPESQESGKKPLSSQIQHVLYSLIRPFPALINSNRICSSSFEGFEEPIKDLGKNMMKFRKRGNELKTNFIEKHLKGYVISTVTWQSTAHVARAISSYLHYDKKPLFGALNTRQRDCLSAMARLCASLSHNMQFLLHAVSDMLRVFLCEPPRPKLAQTPGSPLLSAPSTSSFTPAPAQIPHSGTNFAFLVQLFNPAGPRKNVNLNILQIDILSLAISLMMTIGWTWNNGTQSMNSSSTHQKARLLTPDGSVDEAYVLRLSLLAHYFQIIATHSEADGNDVNMEEEQESQMEVDPVAAQTIRKLYALCHPFDGPLRRVDILWRKMEEGAQSLLRPIALLYHFITLVDPPEALKDPSINSSEPLFRYLGLPHKIEEQISGSMLEKLFTMWSSSIPSDQALRQDLVVQPVRPNLLVELPEKYSQLINQVATFKCPTIPIEESTSNVPTLCLVCGTILCSQAYCCQKIINKQSYGACRYHMSQCSGSVGMFLRVRDCSLVLMTTRKRGCFRPAPYVDEFGEVDQGFRRGNPLHLNPELYQKLKSLWLQQGITEEVVNYNEIDFRNVQYDWGHF

Target ORF information:

RefSeq Version	NM_058830.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058830.5

ORF Insert Sequence:

ATGATTGTCG ATTTAATACA ATCAGCTCGA CAGGGTGAAT GGGCCCAAGT ACGCCAGCTG 
CTGCTCAAGC ACTGGCTTGT ACAGGTCCCA GAGGTGTTCG AAGTGAATTC GGATTTGCCA 
TGGGACAATA CAGCCGCGAA TGAACGAATT CTAGGTTCCC AAGGAGAGAT TCTCCTAGCC 
CCACTGGTCA GTGCATTTGT ACTAGACGTT CGGAACACAA AGAGCACACT GGAAGCAATG 
AATGGCATTG CCGGAGTTGA TCCTGCAAGA CGGGGACAAA TTTGTGGCCA TGTATTTAAA 
AACGGAGAAC TTACGTATAC ATGTCTGGAT TGTGCCACTG ATGGGACATG TGTCATGTGT 
CTTCAGTGTT TTGAAGTTTC AATTCATAAA TCTCATAAAT ACAAAATGCA CTCTTCCTCC 
GGTTCGGGAT ATTGTGACTG TGGTGATGCA GATGCATGGA CAGAAGGTTA CGCATGTGCA 
AATCACGAGA AAAAGGACGA TGAAGAGGCG GCAGTGTTGG CTCCAGAATT GAAAAAACGA 
TGTGAACAGC TGGTGGAAAT CATTTTACAG TTTTCCCTTT CAATGATCAC TCATAAAGAT 
GATCTCAAAC TTCCCGAAAT TTTCGAGAAA ATGAAGCCAG AGGTTACAAA TGAGGCACAG 
CAATATTTGA CGGTTCTTTA CAATGATGAA ACTCACACTT ATGAATCTGT GATCAAAGTT 
CTGGAATTGT ACATACATTG TACAAAGGAT CAGGCGATGC TTGTAGCCAC AATTGTCGAT 
CGAGAAGGTC GAAGTGCTGT GAAACTTGGC AGTAAGGCTG ATTGCACCAA AGCTAAGGAT 
GATGTTCAGA GAAAAACAGC TCGAGATCCA ACGTCAATTC GAAGATCATC GAATCATAAT 
CTACCGTTAA GTGTTAAGGT TATGGATACC ACGCTTTTCG CGCTTCAGAA TTTTTCGATC 
TCCCTTCTTA CTTGGCTCAA CACACAGATG GATGTTTTTC CTCCCCTTCG CGAAATTGTT 
GGAGAAATTC TGCTGAGTTC AAAGTTTGCA CTGAAAAAGA ATTACACGCG GAAAATGAAG 
TCAGAGGATC AACGTCTAGT TGCCGGAATT ATCAGAAATG TTATGGTTTT GCCGGATGAT 
GAAGAGGAAG AACTATTCGC ACTTGATGGA AGAATGGATG TTGATGAAAT GGATGACGAC 
GATATTGGTG GAGAAGCTCT GCAAATGGAA ATTGACGCGG ATGATGAAGA AGAGATAACA 
GCAGCGTTGG CAGGAGTTTC AGAACATCAA GAATCTCCAG GTCCTTCACG AGATTCTTCA 
ACATTCACAA TGCTAGAGAA TATTCTTCTC CAGGATACAC AAATGTGGAA AGCTGGAAGA 
TCGATTTTGC ATCAAATGCT CATGCGGACA GTTTTCATGA TTTATGATCA GAAAGTTCGA 
TTTGCAAAGG CTTTTATGCT ACATTATAAT GAAATTTACG AGGATTTCAT TAAAGATGAT 
CATGAGATGG ATGTTTCAGT TGTTGGGTTA TCAGTTCAAT TCATGACTGT TCCTAGTCTG 
GCTCGGAAAC TTGTCGCCGA AGATCAAGCA TTCAGTGTGA TATCGAAAGC GATTCGGGAT 
CAGACTGATA AATTTGTGAA GTATTACAAT GACGGAAAAA TCGCGAGATT TGATTTCACA 
AGTCGCTCAT TTCCACCAGA ATTAAGAAGA TCCCTTCATA TCACTCGAGA TATGGCCTAT 
ATCCTGAATG CTGTGCCATC GGAAAGCGAC TGGAATCGAG AACTTATCGA TGGATTTGTT 
CAAGGATTCG CTGATTTTCT GCTTTTCCTG CAGCATCTGC AAGGAATGGA TGAGGTGAAA 
CGACAAGCCG TTGAGCATCA AGTGTGGGAA TCTGAGTGGG AAACAGCGTT CAATATCCTT 
TTACGATTGA AAGACGCGAT TTCGATGATT ATTGGATGGG CAGAAACTAA TGAAGAAGTT 
CATAATCGTC TAATGATAAT GTGCCTTGAA TTGATGAATC GTATGCCACC TGTCTATACA 
AAATCCGAGG AGGATACCTA TGAATTGACG GTCACTATCA ACGGAGAATC ATGTCGAATA 
TCACATTTTG ACGTGCTTAA ATCATCCACC TCGGTCCATC AGCCGGTTGT TCGAATTATC 
GCGGGTCTCT TTAGTGCTTC GAATTACACG GGATTTTTGT TGAATAGAAG TAATAATTCA 
CATACGAGTT TGAATCAAGA AAGAATCAAA GAACTTATCA ACTGTAAGGA CGAGACGAAT 
CTCTACGAAC TGAGTCTCCG AGTGCTCGTC CTGTGTGCAC AATCCAACGC GACACTTTGG 
AGAAGAAATG GATTTTCGCT GATAAACCAG ATTCATAATT ATTTCTCTCC ACTCTGTCGA 
AATGAGATGT TCGATCGTGA TGTTCTGATG ATGCAAGTAG GAGCTGCACT CACACCGTCA 
ACAAAATTCA TTTTCCACCT TCTTCATCGA TTCCGTCTCT TTAAATGGGC TACATCCGAG 
TTTGAACAAG ACAAGGCGAA TGAGAAACCA GCGAAACCGG AATCAGAGGA TCTGAGTAAG 
ACGTTGGTGA TGATCGCTGA AGAGTTCATA CAATGTCTGA TTCTGATTCT CTGTGAACGG 
TATACATACG GAGTTGGAAA GACTCGTCCG ATGGATCAGA TGAAACGAGA AGTTATCCAT 
ATTCTTTGTA CTGGATCTCA TACGTTCAGT CATATTCAAC AGAAAATGTC ACATGATATC 
AATTCGAAGC GTCTTTCTCT TCATGAAGCA GTAAATCTGG TTGCAGACTT CCGAAAGCCA 
CTGGCCACCA CAGCTGGACA ATTCCATTGC AAAGAGTCGA GCCTTCCAAC CTATTCACCA 
TTCTTCATGC ATTATTCAAA AAGTGATCAG TCAGCTGCTG AACAGAGTCA GGCAAGAGTT 
CGTGCGAAAA TGGAAAAGTC GGTTCGAGCT TGTGCGCCGC CTATTCTTCC GGATTTTCAA 
ACATTTTTCG AGCGGATTCC CGAAATTCTT ACCACAAATG TTCTTATTCA CGTTGTTCGT 
CTGATTATCG ATAGAACAGC TCGACGTAGC CGTTTCTCAA GTGATCGTCT CTTCCACAAA 
ACACTATATC TGATCGGAAT CGCGTTAAAC GAAGAGGAAA AGAATCCATC TTTCGGATTT 
ACACAACGTG CAGAGGAATC GATCGGTCTT TTGTCGCTTT TAGAAGGACT TGTTGGAAAA 
CCAGAGTCAT CGATTTGTCC GATCCTTCTC GAAGTGACCG TCGAGAAATA CAGAAAATTG 
ATTAAAGCTA GAGCAGGTGT TCCTGAAGCA GCTCCAGCTC CTGAAAACAA GCCAGCACAA 
TCCGAAGAAG AAATCAAAGC GAAGCGAGCT GCAAGGGCGG CGGAAATGAG ACAAAAAGCG 
ATGGCAAAAA TGTCGAATAT GCAGTCAAAG TTTATGAAGA AAATTGAGGA CGAAGAGAAG 
AAAGACGAAT CACAGACACC TTCTGAAAAA TCTGAAACTG TTGTGAAGAA GGACGACTAT 
GATAATAAGC ATTTCTTCGA TGAAGATGTT GTGAAGCAAG TTGGTCACGA TTTTCCGGTT 
TGTATCGGTG CAAATAAGTG GCACGCGGAG CTCGTGAAAC CCCGAACATT GACGTGTATT 
CTGTGTCAAG AGGATGAAAT AATTGCACCG CAACAGGGAA AACCAATGGT TTGCGCAGCG 
TTTATTCAAC AATCTCAACT TTTCACTCAT AAGAACAAAA ATGGAGAGCT CATGACGGCT 
TCAAGTGGGA CAATATCTAC ACGTGATCTT CTAACAGCAC CAGCTACACT TCAATATGGA 
GTTGATGTTT CCACGTGTTC ACATAGTATG CATTATGAGT GTTATCGAAG TCTCGCCGAA 
GCGAATCGAT CTCGCGAGAG TTTGAGAGCC CGTCAAGTTG GTCAACATTC GCATAAAATG 
GTTGATACCG AGAATGGAGA ATATCAGTGT CCGTTGTGCA AACGATTGTC GAATGCGGCG 
ATTCCAGTAT TGCCAGCCTA TCAGCTGACG AATCAAAATG GCTTCTCCAC TGTCTCTGGA 
GCCGGAAAGG AGAACTTTGA CACATGGGTG GCACGAGTCA AACGGAATCT GGAAATGCCG 
CTCAGTTCGG AATCTGTCAG CAAGAAGGGT CATAGTAGGA AGAGATCACA TTCCGAACGA 
TCACTGCTGG ATCTTGAGAA GCTCAGCAAG GATCCGGATA CTGCCAACAC GTCTGCTGGT 
GTATTACAAT TTGGAGCCAT GGAAATGAGT AGTGCTACAC ATATGCCAGC GTCTGCCGAA 
TCTCAAATGC TTATGACAAC GTCACCTTCT CAAGATGACG TAGAATTCTA TAATGAATTG 
GCTGCGATGT TTGTGGATCA AGATGTGAAT AATACTACAT CACCGGCAGC TACACCTGAA 
ACAATCCCAG CTATAGGTTC ATCGTCTCGA ATTCCTGAAT CTCAAGAAAG TGGAAAAAAG 
CCGTTGAGTA GTCAAATCCA GCATGTACTC TATTCACTAA TTCGACCATT CCCCGCGTTG 
ATCAACTCGA ATCGTATATG TTCTTCTTCT TTTGAAGGTT TTGAGGAACC AATAAAAGAT 
CTTGGGAAGA ATATGATGAA GTTCCGGAAA CGTGGCAATG AGCTGAAGAC GAATTTCATA 
GAAAAACATC TGAAGGGGTA CGTGATTTCG ACAGTTACCT GGCAAAGTAC TGCTCACGTG 
GCTCGAGCCA TTTCATCGTA CCTCCACTAC GACAAAAAAC CACTTTTTGG AGCATTAAAC 
ACTCGTCAAA GAGATTGTCT TTCTGCAATG GCTCGTCTAT GTGCATCACT ATCACATAAT 
ATGCAGTTCT TGCTTCACGC AGTCTCCGAC ATGCTTCGAG TATTCCTTTG TGAACCACCG 
AGACCGAAAT TAGCTCAAAC ACCGGGATCT CCGTTGCTAA GTGCTCCGTC GACATCTTCG 
TTCACACCTG CACCTGCTCA AATTCCGCAT TCGGGTACAA ACTTTGCATT TTTGGTGCAG 
TTATTCAATC CAGCTGGTCC GAGGAAGAAT GTGAATCTGA ACATTTTGCA AATCGATATT 
TTGAGTTTGG CGATTTCTCT GATGATGACA ATCGGATGGA CGTGGAACAA CGGAACACAA 
TCCATGAACT CCTCGTCAAC GCATCAAAAA GCTCGATTAC TGACACCTGA TGGATCTGTA 
GATGAGGCAT ATGTTCTTCG TTTGTCACTT TTGGCACATT ATTTCCAAAT CATCGCAACA 
CACTCAGAAG CGGACGGAAA TGATGTTAAT ATGGAAGAGG AACAGGAATC ACAGATGGAA 
GTGGATCCGG TTGCTGCTCA GACGATCAGG AAGCTTTACG CTCTTTGTCA TCCATTTGAT 
GGGCCATTGC GACGAGTTGA TATTCTTTGG AGGAAGATGG AAGAAGGTGC TCAATCTCTA 
CTTCGTCCGA TTGCTCTTTT GTATCATTTC ATCACACTTG TCGATCCACC AGAGGCTCTG 
AAAGATCCGT CAATCAATAG TTCGGAGCCA TTATTCCGCT ATCTGGGTTT ACCGCATAAG 
ATTGAAGAAC AAATCAGTGG AAGTATGCTC GAAAAGTTGT TCACAATGTG GTCATCATCG 
ATTCCAAGTG ATCAAGCTCT TCGACAGGAT CTTGTTGTTC AACCAGTTCG ACCGAATCTT 
CTTGTTGAAT TGCCAGAGAA GTATAGTCAA TTAATAAATC AAGTTGCCAC ATTCAAATGT 
CCAACGATAC CGATTGAAGA GTCAACGTCA AATGTTCCGA CACTATGTCT AGTGTGCGGA 
ACTATCCTCT GCTCACAGGC ATATTGCTGT CAGAAAATTA TCAACAAACA ATCATATGGA 
GCATGTCGTT ATCATATGTC ACAGTGTTCC GGATCAGTGG GAATGTTTCT ACGAGTTCGA 
GACTGTAGCC TTGTATTGAT GACGACACGA AAACGCGGTT GCTTCCGTCC AGCACCATAT 
GTCGACGAAT TCGGAGAGGT TGATCAAGGA TTCCGACGTG GAAATCCCCT TCACCTGAAC 
CCTGAACTCT ATCAGAAGCT GAAGTCTTTG TGGCTTCAGC AGGGTATCAC TGAAGAAGTT 
GTGAACTACA ATGAAATTGA CTTCCGCAAT GTGCAATACG ATTGGGGTCA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr-1 ( NM_058830.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491231.2 Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), partial mRNA.

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CloneID	OCa336635
Clone ID Related Accession (Same CDS sequence)	NM_058830.5 , NM_058830.4
Accession Version	NM_058830.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	E3 ubiquitin-protein ligase ubr-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_058830.3. COMPLETENESS: incomplete on both ends.

ATGATTGTCG ATTTAATACA ATCAGCTCGA CAGGGTGAAT GGGCCCAAGT ACGCCAGCTG 
CTGCTCAAGC ACTGGCTTGT ACAGGTCCCA GAGGTGTTCG AAGTGAATTC GGATTTGCCA 
TGGGACAATA CAGCCGCGAA TGAACGAATT CTAGGTTCCC AAGGAGAGAT TCTCCTAGCC 
CCACTGGTCA GTGCATTTGT ACTAGACGTT CGGAACACAA AGAGCACACT GGAAGCAATG 
AATGGCATTG CCGGAGTTGA TCCTGCAAGA CGGGGACAAA TTTGTGGCCA TGTATTTAAA 
AACGGAGAAC TTACGTATAC ATGTCTGGAT TGTGCCACTG ATGGGACATG TGTCATGTGT 
CTTCAGTGTT TTGAAGTTTC AATTCATAAA TCTCATAAAT ACAAAATGCA CTCTTCCTCC 
GGTTCGGGAT ATTGTGACTG TGGTGATGCA GATGCATGGA CAGAAGGTTA CGCATGTGCA 
AATCACGAGA AAAAGGACGA TGAAGAGGCG GCAGTGTTGG CTCCAGAATT GAAAAAACGA 
TGTGAACAGC TGGTGGAAAT CATTTTACAG TTTTCCCTTT CAATGATCAC TCATAAAGAT 
GATCTCAAAC TTCCCGAAAT TTTCGAGAAA ATGAAGCCAG AGGTTACAAA TGAGGCACAG 
CAATATTTGA CGGTTCTTTA CAATGATGAA ACTCACACTT ATGAATCTGT GATCAAAGTT 
CTGGAATTGT ACATACATTG TACAAAGGAT CAGGCGATGC TTGTAGCCAC AATTGTCGAT 
CGAGAAGGTC GAAGTGCTGT GAAACTTGGC AGTAAGGCTG ATTGCACCAA AGCTAAGGAT 
GATGTTCAGA GAAAAACAGC TCGAGATCCA ACGTCAATTC GAAGATCATC GAATCATAAT 
CTACCGTTAA GTGTTAAGGT TATGGATACC ACGCTTTTCG CGCTTCAGAA TTTTTCGATC 
TCCCTTCTTA CTTGGCTCAA CACACAGATG GATGTTTTTC CTCCCCTTCG CGAAATTGTT 
GGAGAAATTC TGCTGAGTTC AAAGTTTGCA CTGAAAAAGA ATTACACGCG GAAAATGAAG 
TCAGAGGATC AACGTCTAGT TGCCGGAATT ATCAGAAATG TTATGGTTTT GCCGGATGAT 
GAAGAGGAAG AACTATTCGC ACTTGATGGA AGAATGGATG TTGATGAAAT GGATGACGAC 
GATATTGGTG GAGAAGCTCT GCAAATGGAA ATTGACGCGG ATGATGAAGA AGAGATAACA 
GCAGCGTTGG CAGGAGTTTC AGAACATCAA GAATCTCCAG GTCCTTCACG AGATTCTTCA 
ACATTCACAA TGCTAGAGAA TATTCTTCTC CAGGATACAC AAATGTGGAA AGCTGGAAGA 
TCGATTTTGC ATCAAATGCT CATGCGGACA GTTTTCATGA TTTATGATCA GAAAGTTCGA 
TTTGCAAAGG CTTTTATGCT ACATTATAAT GAAATTTACG AGGATTTCAT TAAAGATGAT 
CATGAGATGG ATGTTTCAGT TGTTGGGTTA TCAGTTCAAT TCATGACTGT TCCTAGTCTG 
GCTCGGAAAC TTGTCGCCGA AGATCAAGCA TTCAGTGTGA TATCGAAAGC GATTCGGGAT 
CAGACTGATA AATTTGTGAA GTATTACAAT GACGGAAAAA TCGCGAGATT TGATTTCACA 
AGTCGCTCAT TTCCACCAGA ATTAAGAAGA TCCCTTCATA TCACTCGAGA TATGGCCTAT 
ATCCTGAATG CTGTGCCATC GGAAAGCGAC TGGAATCGAG AACTTATCGA TGGATTTGTT 
CAAGGATTCG CTGATTTTCT GCTTTTCCTG CAGCATCTGC AAGGAATGGA TGAGGTGAAA 
CGACAAGCCG TTGAGCATCA AGTGTGGGAA TCTGAGTGGG AAACAGCGTT CAATATCCTT 
TTACGATTGA AAGACGCGAT TTCGATGATT ATTGGATGGG CAGAAACTAA TGAAGAAGTT 
CATAATCGTC TAATGATAAT GTGCCTTGAA TTGATGAATC GTATGCCACC TGTCTATACA 
AAATCCGAGG AGGATACCTA TGAATTGACG GTCACTATCA ACGGAGAATC ATGTCGAATA 
TCACATTTTG ACGTGCTTAA ATCATCCACC TCGGTCCATC AGCCGGTTGT TCGAATTATC 
GCGGGTCTCT TTAGTGCTTC GAATTACACG GGATTTTTGT TGAATAGAAG TAATAATTCA 
CATACGAGTT TGAATCAAGA AAGAATCAAA GAACTTATCA ACTGTAAGGA CGAGACGAAT 
CTCTACGAAC TGAGTCTCCG AGTGCTCGTC CTGTGTGCAC AATCCAACGC GACACTTTGG 
AGAAGAAATG GATTTTCGCT GATAAACCAG ATTCATAATT ATTTCTCTCC ACTCTGTCGA 
AATGAGATGT TCGATCGTGA TGTTCTGATG ATGCAAGTAG GAGCTGCACT CACACCGTCA 
ACAAAATTCA TTTTCCACCT TCTTCATCGA TTCCGTCTCT TTAAATGGGC TACATCCGAG 
TTTGAACAAG ACAAGGCGAA TGAGAAACCA GCGAAACCGG AATCAGAGGA TCTGAGTAAG 
ACGTTGGTGA TGATCGCTGA AGAGTTCATA CAATGTCTGA TTCTGATTCT CTGTGAACGG 
TATACATACG GAGTTGGAAA GACTCGTCCG ATGGATCAGA TGAAACGAGA AGTTATCCAT 
ATTCTTTGTA CTGGATCTCA TACGTTCAGT CATATTCAAC AGAAAATGTC ACATGATATC 
AATTCGAAGC GTCTTTCTCT TCATGAAGCA GTAAATCTGG TTGCAGACTT CCGAAAGCCA 
CTGGCCACCA CAGCTGGACA ATTCCATTGC AAAGAGTCGA GCCTTCCAAC CTATTCACCA 
TTCTTCATGC ATTATTCAAA AAGTGATCAG TCAGCTGCTG AACAGAGTCA GGCAAGAGTT 
CGTGCGAAAA TGGAAAAGTC GGTTCGAGCT TGTGCGCCGC CTATTCTTCC GGATTTTCAA 
ACATTTTTCG AGCGGATTCC CGAAATTCTT ACCACAAATG TTCTTATTCA CGTTGTTCGT 
CTGATTATCG ATAGAACAGC TCGACGTAGC CGTTTCTCAA GTGATCGTCT CTTCCACAAA 
ACACTATATC TGATCGGAAT CGCGTTAAAC GAAGAGGAAA AGAATCCATC TTTCGGATTT 
ACACAACGTG CAGAGGAATC GATCGGTCTT TTGTCGCTTT TAGAAGGACT TGTTGGAAAA 
CCAGAGTCAT CGATTTGTCC GATCCTTCTC GAAGTGACCG TCGAGAAATA CAGAAAATTG 
ATTAAAGCTA GAGCAGGTGT TCCTGAAGCA GCTCCAGCTC CTGAAAACAA GCCAGCACAA 
TCCGAAGAAG AAATCAAAGC GAAGCGAGCT GCAAGGGCGG CGGAAATGAG ACAAAAAGCG 
ATGGCAAAAA TGTCGAATAT GCAGTCAAAG TTTATGAAGA AAATTGAGGA CGAAGAGAAG 
AAAGACGAAT CACAGACACC TTCTGAAAAA TCTGAAACTG TTGTGAAGAA GGACGACTAT 
GATAATAAGC ATTTCTTCGA TGAAGATGTT GTGAAGCAAG TTGGTCACGA TTTTCCGGTT 
TGTATCGGTG CAAATAAGTG GCACGCGGAG CTCGTGAAAC CCCGAACATT GACGTGTATT 
CTGTGTCAAG AGGATGAAAT AATTGCACCG CAACAGGGAA AACCAATGGT TTGCGCAGCG 
TTTATTCAAC AATCTCAACT TTTCACTCAT AAGAACAAAA ATGGAGAGCT CATGACGGCT 
TCAAGTGGGA CAATATCTAC ACGTGATCTT CTAACAGCAC CAGCTACACT TCAATATGGA 
GTTGATGTTT CCACGTGTTC ACATAGTATG CATTATGAGT GTTATCGAAG TCTCGCCGAA 
GCGAATCGAT CTCGCGAGAG TTTGAGAGCC CGTCAAGTTG GTCAACATTC GCATAAAATG 
GTTGATACCG AGAATGGAGA ATATCAGTGT CCGTTGTGCA AACGATTGTC GAATGCGGCG 
ATTCCAGTAT TGCCAGCCTA TCAGCTGACG AATCAAAATG GCTTCTCCAC TGTCTCTGGA 
GCCGGAAAGG AGAACTTTGA CACATGGGTG GCACGAGTCA AACGGAATCT GGAAATGCCG 
CTCAGTTCGG AATCTGTCAG CAAGAAGGGT CATAGTAGGA AGAGATCACA TTCCGAACGA 
TCACTGCTGG ATCTTGAGAA GCTCAGCAAG GATCCGGATA CTGCCAACAC GTCTGCTGGT 
GTATTACAAT TTGGAGCCAT GGAAATGAGT AGTGCTACAC ATATGCCAGC GTCTGCCGAA 
TCTCAAATGC TTATGACAAC GTCACCTTCT CAAGATGACG TAGAATTCTA TAATGAATTG 
GCTGCGATGT TTGTGGATCA AGATGTGAAT AATACTACAT CACCGGCAGC TACACCTGAA 
ACAATCCCAG CTATAGGTTC ATCGTCTCGA ATTCCTGAAT CTCAAGAAAG TGGAAAAAAG 
CCGTTGAGTA GTCAAATCCA GCATGTACTC TATTCACTAA TTCGACCATT CCCCGCGTTG 
ATCAACTCGA ATCGTATATG TTCTTCTTCT TTTGAAGGTT TTGAGGAACC AATAAAAGAT 
CTTGGGAAGA ATATGATGAA GTTCCGGAAA CGTGGCAATG AGCTGAAGAC GAATTTCATA 
GAAAAACATC TGAAGGGGTA CGTGATTTCG ACAGTTACCT GGCAAAGTAC TGCTCACGTG 
GCTCGAGCCA TTTCATCGTA CCTCCACTAC GACAAAAAAC CACTTTTTGG AGCATTAAAC 
ACTCGTCAAA GAGATTGTCT TTCTGCAATG GCTCGTCTAT GTGCATCACT ATCACATAAT 
ATGCAGTTCT TGCTTCACGC AGTCTCCGAC ATGCTTCGAG TATTCCTTTG TGAACCACCG 
AGACCGAAAT TAGCTCAAAC ACCGGGATCT CCGTTGCTAA GTGCTCCGTC GACATCTTCG 
TTCACACCTG CACCTGCTCA AATTCCGCAT TCGGGTACAA ACTTTGCATT TTTGGTGCAG 
TTATTCAATC CAGCTGGTCC GAGGAAGAAT GTGAATCTGA ACATTTTGCA AATCGATATT 
TTGAGTTTGG CGATTTCTCT GATGATGACA ATCGGATGGA CGTGGAACAA CGGAACACAA 
TCCATGAACT CCTCGTCAAC GCATCAAAAA GCTCGATTAC TGACACCTGA TGGATCTGTA 
GATGAGGCAT ATGTTCTTCG TTTGTCACTT TTGGCACATT ATTTCCAAAT CATCGCAACA 
CACTCAGAAG CGGACGGAAA TGATGTTAAT ATGGAAGAGG AACAGGAATC ACAGATGGAA 
GTGGATCCGG TTGCTGCTCA GACGATCAGG AAGCTTTACG CTCTTTGTCA TCCATTTGAT 
GGGCCATTGC GACGAGTTGA TATTCTTTGG AGGAAGATGG AAGAAGGTGC TCAATCTCTA 
CTTCGTCCGA TTGCTCTTTT GTATCATTTC ATCACACTTG TCGATCCACC AGAGGCTCTG 
AAAGATCCGT CAATCAATAG TTCGGAGCCA TTATTCCGCT ATCTGGGTTT ACCGCATAAG 
ATTGAAGAAC AAATCAGTGG AAGTATGCTC GAAAAGTTGT TCACAATGTG GTCATCATCG 
ATTCCAAGTG ATCAAGCTCT TCGACAGGAT CTTGTTGTTC AACCAGTTCG ACCGAATCTT 
CTTGTTGAAT TGCCAGAGAA GTATAGTCAA TTAATAAATC AAGTTGCCAC ATTCAAATGT 
CCAACGATAC CGATTGAAGA GTCAACGTCA AATGTTCCGA CACTATGTCT AGTGTGCGGA 
ACTATCCTCT GCTCACAGGC ATATTGCTGT CAGAAAATTA TCAACAAACA ATCATATGGA 
GCATGTCGTT ATCATATGTC ACAGTGTTCC GGATCAGTGG GAATGTTTCT ACGAGTTCGA 
GACTGTAGCC TTGTATTGAT GACGACACGA AAACGCGGTT GCTTCCGTCC AGCACCATAT 
GTCGACGAAT TCGGAGAGGT TGATCAAGGA TTCCGACGTG GAAATCCCCT TCACCTGAAC 
CCTGAACTCT ATCAGAAGCT GAAGTCTTTG TGGCTTCAGC AGGGTATCAC TGAAGAAGTT 
GTGAACTACA ATGAAATTGA CTTCCGCAAT GTGCAATACG ATTGGGGTCA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491231.2
CDS	1..6177
Translation	MIVDLIQSARQGEWAQVRQLLLKHWLVQVPEVFEVNSDLPWDNTAANERILGSQGEILLAPLVSAFVLDVRNTKSTLEAMNGIAGVDPARRGQICGHVFKNGELTYTCLDCATDGTCVMCLQCFEVSIHKSHKYKMHSSSGSGYCDCGDADAWTEGYACANHEKKDDEEAAVLAPELKKRCEQLVEIILQFSLSMITHKDDLKLPEIFEKMKPEVTNEAQQYLTVLYNDETHTYESVIKVLELYIHCTKDQAMLVATIVDREGRSAVKLGSKADCTKAKDDVQRKTARDPTSIRRSSNHNLPLSVKVMDTTLFALQNFSISLLTWLNTQMDVFPPLREIVGEILLSSKFALKKNYTRKMKSEDQRLVAGIIRNVMVLPDDEEEELFALDGRMDVDEMDDDDIGGEALQMEIDADDEEEITAALAGVSEHQESPGPSRDSSTFTMLENILLQDTQMWKAGRSILHQMLMRTVFMIYDQKVRFAKAFMLHYNEIYEDFIKDDHEMDVSVVGLSVQFMTVPSLARKLVAEDQAFSVISKAIRDQTDKFVKYYNDGKIARFDFTSRSFPPELRRSLHITRDMAYILNAVPSESDWNRELIDGFVQGFADFLLFLQHLQGMDEVKRQAVEHQVWESEWETAFNILLRLKDAISMIIGWAETNEEVHNRLMIMCLELMNRMPPVYTKSEEDTYELTVTINGESCRISHFDVLKSSTSVHQPVVRIIAGLFSASNYTGFLLNRSNNSHTSLNQERIKELINCKDETNLYELSLRVLVLCAQSNATLWRRNGFSLINQIHNYFSPLCRNEMFDRDVLMMQVGAALTPSTKFIFHLLHRFRLFKWATSEFEQDKANEKPAKPESEDLSKTLVMIAEEFIQCLILILCERYTYGVGKTRPMDQMKREVIHILCTGSHTFSHIQQKMSHDINSKRLSLHEAVNLVADFRKPLATTAGQFHCKESSLPTYSPFFMHYSKSDQSAAEQSQARVRAKMEKSVRACAPPILPDFQTFFERIPEILTTNVLIHVVRLIIDRTARRSRFSSDRLFHKTLYLIGIALNEEEKNPSFGFTQRAEESIGLLSLLEGLVGKPESSICPILLEVTVEKYRKLIKARAGVPEAAPAPENKPAQSEEEIKAKRAARAAEMRQKAMAKMSNMQSKFMKKIEDEEKKDESQTPSEKSETVVKKDDYDNKHFFDEDVVKQVGHDFPVCIGANKWHAELVKPRTLTCILCQEDEIIAPQQGKPMVCAAFIQQSQLFTHKNKNGELMTASSGTISTRDLLTAPATLQYGVDVSTCSHSMHYECYRSLAEANRSRESLRARQVGQHSHKMVDTENGEYQCPLCKRLSNAAIPVLPAYQLTNQNGFSTVSGAGKENFDTWVARVKRNLEMPLSSESVSKKGHSRKRSHSERSLLDLEKLSKDPDTANTSAGVLQFGAMEMSSATHMPASAESQMLMTTSPSQDDVEFYNELAAMFVDQDVNNTTSPAATPETIPAIGSSSRIPESQESGKKPLSSQIQHVLYSLIRPFPALINSNRICSSSFEGFEEPIKDLGKNMMKFRKRGNELKTNFIEKHLKGYVISTVTWQSTAHVARAISSYLHYDKKPLFGALNTRQRDCLSAMARLCASLSHNMQFLLHAVSDMLRVFLCEPPRPKLAQTPGSPLLSAPSTSSFTPAPAQIPHSGTNFAFLVQLFNPAGPRKNVNLNILQIDILSLAISLMMTIGWTWNNGTQSMNSSSTHQKARLLTPDGSVDEAYVLRLSLLAHYFQIIATHSEADGNDVNMEEEQESQMEVDPVAAQTIRKLYALCHPFDGPLRRVDILWRKMEEGAQSLLRPIALLYHFITLVDPPEALKDPSINSSEPLFRYLGLPHKIEEQISGSMLEKLFTMWSSSIPSDQALRQDLVVQPVRPNLLVELPEKYSQLINQVATFKCPTIPIEESTSNVPTLCLVCGTILCSQAYCCQKIINKQSYGACRYHMSQCSGSVGMFLRVRDCSLVLMTTRKRGCFRPAPYVDEFGEVDQGFRRGNPLHLNPELYQKLKSLWLQQGITEEVVNYNEIDFRNVQYDWGHF

Target ORF information:

RefSeq Version	NM_058830.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans E3 ubiquitin-protein ligase ubr-1 (ubr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058830.4

ORF Insert Sequence:

ATGATTGTCG ATTTAATACA ATCAGCTCGA CAGGGTGAAT GGGCCCAAGT ACGCCAGCTG 
CTGCTCAAGC ACTGGCTTGT ACAGGTCCCA GAGGTGTTCG AAGTGAATTC GGATTTGCCA 
TGGGACAATA CAGCCGCGAA TGAACGAATT CTAGGTTCCC AAGGAGAGAT TCTCCTAGCC 
CCACTGGTCA GTGCATTTGT ACTAGACGTT CGGAACACAA AGAGCACACT GGAAGCAATG 
AATGGCATTG CCGGAGTTGA TCCTGCAAGA CGGGGACAAA TTTGTGGCCA TGTATTTAAA 
AACGGAGAAC TTACGTATAC ATGTCTGGAT TGTGCCACTG ATGGGACATG TGTCATGTGT 
CTTCAGTGTT TTGAAGTTTC AATTCATAAA TCTCATAAAT ACAAAATGCA CTCTTCCTCC 
GGTTCGGGAT ATTGTGACTG TGGTGATGCA GATGCATGGA CAGAAGGTTA CGCATGTGCA 
AATCACGAGA AAAAGGACGA TGAAGAGGCG GCAGTGTTGG CTCCAGAATT GAAAAAACGA 
TGTGAACAGC TGGTGGAAAT CATTTTACAG TTTTCCCTTT CAATGATCAC TCATAAAGAT 
GATCTCAAAC TTCCCGAAAT TTTCGAGAAA ATGAAGCCAG AGGTTACAAA TGAGGCACAG 
CAATATTTGA CGGTTCTTTA CAATGATGAA ACTCACACTT ATGAATCTGT GATCAAAGTT 
CTGGAATTGT ACATACATTG TACAAAGGAT CAGGCGATGC TTGTAGCCAC AATTGTCGAT 
CGAGAAGGTC GAAGTGCTGT GAAACTTGGC AGTAAGGCTG ATTGCACCAA AGCTAAGGAT 
GATGTTCAGA GAAAAACAGC TCGAGATCCA ACGTCAATTC GAAGATCATC GAATCATAAT 
CTACCGTTAA GTGTTAAGGT TATGGATACC ACGCTTTTCG CGCTTCAGAA TTTTTCGATC 
TCCCTTCTTA CTTGGCTCAA CACACAGATG GATGTTTTTC CTCCCCTTCG CGAAATTGTT 
GGAGAAATTC TGCTGAGTTC AAAGTTTGCA CTGAAAAAGA ATTACACGCG GAAAATGAAG 
TCAGAGGATC AACGTCTAGT TGCCGGAATT ATCAGAAATG TTATGGTTTT GCCGGATGAT 
GAAGAGGAAG AACTATTCGC ACTTGATGGA AGAATGGATG TTGATGAAAT GGATGACGAC 
GATATTGGTG GAGAAGCTCT GCAAATGGAA ATTGACGCGG ATGATGAAGA AGAGATAACA 
GCAGCGTTGG CAGGAGTTTC AGAACATCAA GAATCTCCAG GTCCTTCACG AGATTCTTCA 
ACATTCACAA TGCTAGAGAA TATTCTTCTC CAGGATACAC AAATGTGGAA AGCTGGAAGA 
TCGATTTTGC ATCAAATGCT CATGCGGACA GTTTTCATGA TTTATGATCA GAAAGTTCGA 
TTTGCAAAGG CTTTTATGCT ACATTATAAT GAAATTTACG AGGATTTCAT TAAAGATGAT 
CATGAGATGG ATGTTTCAGT TGTTGGGTTA TCAGTTCAAT TCATGACTGT TCCTAGTCTG 
GCTCGGAAAC TTGTCGCCGA AGATCAAGCA TTCAGTGTGA TATCGAAAGC GATTCGGGAT 
CAGACTGATA AATTTGTGAA GTATTACAAT GACGGAAAAA TCGCGAGATT TGATTTCACA 
AGTCGCTCAT TTCCACCAGA ATTAAGAAGA TCCCTTCATA TCACTCGAGA TATGGCCTAT 
ATCCTGAATG CTGTGCCATC GGAAAGCGAC TGGAATCGAG AACTTATCGA TGGATTTGTT 
CAAGGATTCG CTGATTTTCT GCTTTTCCTG CAGCATCTGC AAGGAATGGA TGAGGTGAAA 
CGACAAGCCG TTGAGCATCA AGTGTGGGAA TCTGAGTGGG AAACAGCGTT CAATATCCTT 
TTACGATTGA AAGACGCGAT TTCGATGATT ATTGGATGGG CAGAAACTAA TGAAGAAGTT 
CATAATCGTC TAATGATAAT GTGCCTTGAA TTGATGAATC GTATGCCACC TGTCTATACA 
AAATCCGAGG AGGATACCTA TGAATTGACG GTCACTATCA ACGGAGAATC ATGTCGAATA 
TCACATTTTG ACGTGCTTAA ATCATCCACC TCGGTCCATC AGCCGGTTGT TCGAATTATC 
GCGGGTCTCT TTAGTGCTTC GAATTACACG GGATTTTTGT TGAATAGAAG TAATAATTCA 
CATACGAGTT TGAATCAAGA AAGAATCAAA GAACTTATCA ACTGTAAGGA CGAGACGAAT 
CTCTACGAAC TGAGTCTCCG AGTGCTCGTC CTGTGTGCAC AATCCAACGC GACACTTTGG 
AGAAGAAATG GATTTTCGCT GATAAACCAG ATTCATAATT ATTTCTCTCC ACTCTGTCGA 
AATGAGATGT TCGATCGTGA TGTTCTGATG ATGCAAGTAG GAGCTGCACT CACACCGTCA 
ACAAAATTCA TTTTCCACCT TCTTCATCGA TTCCGTCTCT TTAAATGGGC TACATCCGAG 
TTTGAACAAG ACAAGGCGAA TGAGAAACCA GCGAAACCGG AATCAGAGGA TCTGAGTAAG 
ACGTTGGTGA TGATCGCTGA AGAGTTCATA CAATGTCTGA TTCTGATTCT CTGTGAACGG 
TATACATACG GAGTTGGAAA GACTCGTCCG ATGGATCAGA TGAAACGAGA AGTTATCCAT 
ATTCTTTGTA CTGGATCTCA TACGTTCAGT CATATTCAAC AGAAAATGTC ACATGATATC 
AATTCGAAGC GTCTTTCTCT TCATGAAGCA GTAAATCTGG TTGCAGACTT CCGAAAGCCA 
CTGGCCACCA CAGCTGGACA ATTCCATTGC AAAGAGTCGA GCCTTCCAAC CTATTCACCA 
TTCTTCATGC ATTATTCAAA AAGTGATCAG TCAGCTGCTG AACAGAGTCA GGCAAGAGTT 
CGTGCGAAAA TGGAAAAGTC GGTTCGAGCT TGTGCGCCGC CTATTCTTCC GGATTTTCAA 
ACATTTTTCG AGCGGATTCC CGAAATTCTT ACCACAAATG TTCTTATTCA CGTTGTTCGT 
CTGATTATCG ATAGAACAGC TCGACGTAGC CGTTTCTCAA GTGATCGTCT CTTCCACAAA 
ACACTATATC TGATCGGAAT CGCGTTAAAC GAAGAGGAAA AGAATCCATC TTTCGGATTT 
ACACAACGTG CAGAGGAATC GATCGGTCTT TTGTCGCTTT TAGAAGGACT TGTTGGAAAA 
CCAGAGTCAT CGATTTGTCC GATCCTTCTC GAAGTGACCG TCGAGAAATA CAGAAAATTG 
ATTAAAGCTA GAGCAGGTGT TCCTGAAGCA GCTCCAGCTC CTGAAAACAA GCCAGCACAA 
TCCGAAGAAG AAATCAAAGC GAAGCGAGCT GCAAGGGCGG CGGAAATGAG ACAAAAAGCG 
ATGGCAAAAA TGTCGAATAT GCAGTCAAAG TTTATGAAGA AAATTGAGGA CGAAGAGAAG 
AAAGACGAAT CACAGACACC TTCTGAAAAA TCTGAAACTG TTGTGAAGAA GGACGACTAT 
GATAATAAGC ATTTCTTCGA TGAAGATGTT GTGAAGCAAG TTGGTCACGA TTTTCCGGTT 
TGTATCGGTG CAAATAAGTG GCACGCGGAG CTCGTGAAAC CCCGAACATT GACGTGTATT 
CTGTGTCAAG AGGATGAAAT AATTGCACCG CAACAGGGAA AACCAATGGT TTGCGCAGCG 
TTTATTCAAC AATCTCAACT TTTCACTCAT AAGAACAAAA ATGGAGAGCT CATGACGGCT 
TCAAGTGGGA CAATATCTAC ACGTGATCTT CTAACAGCAC CAGCTACACT TCAATATGGA 
GTTGATGTTT CCACGTGTTC ACATAGTATG CATTATGAGT GTTATCGAAG TCTCGCCGAA 
GCGAATCGAT CTCGCGAGAG TTTGAGAGCC CGTCAAGTTG GTCAACATTC GCATAAAATG 
GTTGATACCG AGAATGGAGA ATATCAGTGT CCGTTGTGCA AACGATTGTC GAATGCGGCG 
ATTCCAGTAT TGCCAGCCTA TCAGCTGACG AATCAAAATG GCTTCTCCAC TGTCTCTGGA 
GCCGGAAAGG AGAACTTTGA CACATGGGTG GCACGAGTCA AACGGAATCT GGAAATGCCG 
CTCAGTTCGG AATCTGTCAG CAAGAAGGGT CATAGTAGGA AGAGATCACA TTCCGAACGA 
TCACTGCTGG ATCTTGAGAA GCTCAGCAAG GATCCGGATA CTGCCAACAC GTCTGCTGGT 
GTATTACAAT TTGGAGCCAT GGAAATGAGT AGTGCTACAC ATATGCCAGC GTCTGCCGAA 
TCTCAAATGC TTATGACAAC GTCACCTTCT CAAGATGACG TAGAATTCTA TAATGAATTG 
GCTGCGATGT TTGTGGATCA AGATGTGAAT AATACTACAT CACCGGCAGC TACACCTGAA 
ACAATCCCAG CTATAGGTTC ATCGTCTCGA ATTCCTGAAT CTCAAGAAAG TGGAAAAAAG 
CCGTTGAGTA GTCAAATCCA GCATGTACTC TATTCACTAA TTCGACCATT CCCCGCGTTG 
ATCAACTCGA ATCGTATATG TTCTTCTTCT TTTGAAGGTT TTGAGGAACC AATAAAAGAT 
CTTGGGAAGA ATATGATGAA GTTCCGGAAA CGTGGCAATG AGCTGAAGAC GAATTTCATA 
GAAAAACATC TGAAGGGGTA CGTGATTTCG ACAGTTACCT GGCAAAGTAC TGCTCACGTG 
GCTCGAGCCA TTTCATCGTA CCTCCACTAC GACAAAAAAC CACTTTTTGG AGCATTAAAC 
ACTCGTCAAA GAGATTGTCT TTCTGCAATG GCTCGTCTAT GTGCATCACT ATCACATAAT 
ATGCAGTTCT TGCTTCACGC AGTCTCCGAC ATGCTTCGAG TATTCCTTTG TGAACCACCG 
AGACCGAAAT TAGCTCAAAC ACCGGGATCT CCGTTGCTAA GTGCTCCGTC GACATCTTCG 
TTCACACCTG CACCTGCTCA AATTCCGCAT TCGGGTACAA ACTTTGCATT TTTGGTGCAG 
TTATTCAATC CAGCTGGTCC GAGGAAGAAT GTGAATCTGA ACATTTTGCA AATCGATATT 
TTGAGTTTGG CGATTTCTCT GATGATGACA ATCGGATGGA CGTGGAACAA CGGAACACAA 
TCCATGAACT CCTCGTCAAC GCATCAAAAA GCTCGATTAC TGACACCTGA TGGATCTGTA 
GATGAGGCAT ATGTTCTTCG TTTGTCACTT TTGGCACATT ATTTCCAAAT CATCGCAACA 
CACTCAGAAG CGGACGGAAA TGATGTTAAT ATGGAAGAGG AACAGGAATC ACAGATGGAA 
GTGGATCCGG TTGCTGCTCA GACGATCAGG AAGCTTTACG CTCTTTGTCA TCCATTTGAT 
GGGCCATTGC GACGAGTTGA TATTCTTTGG AGGAAGATGG AAGAAGGTGC TCAATCTCTA 
CTTCGTCCGA TTGCTCTTTT GTATCATTTC ATCACACTTG TCGATCCACC AGAGGCTCTG 
AAAGATCCGT CAATCAATAG TTCGGAGCCA TTATTCCGCT ATCTGGGTTT ACCGCATAAG 
ATTGAAGAAC AAATCAGTGG AAGTATGCTC GAAAAGTTGT TCACAATGTG GTCATCATCG 
ATTCCAAGTG ATCAAGCTCT TCGACAGGAT CTTGTTGTTC AACCAGTTCG ACCGAATCTT 
CTTGTTGAAT TGCCAGAGAA GTATAGTCAA TTAATAAATC AAGTTGCCAC ATTCAAATGT 
CCAACGATAC CGATTGAAGA GTCAACGTCA AATGTTCCGA CACTATGTCT AGTGTGCGGA 
ACTATCCTCT GCTCACAGGC ATATTGCTGT CAGAAAATTA TCAACAAACA ATCATATGGA 
GCATGTCGTT ATCATATGTC ACAGTGTTCC GGATCAGTGG GAATGTTTCT ACGAGTTCGA 
GACTGTAGCC TTGTATTGAT GACGACACGA AAACGCGGTT GCTTCCGTCC AGCACCATAT 
GTCGACGAAT TCGGAGAGGT TGATCAAGGA TTCCGACGTG GAAATCCCCT TCACCTGAAC 
CCTGAACTCT ATCAGAAGCT GAAGTCTTTG TGGCTTCAGC AGGGTATCAC TGAAGAAGTT 
GTGAACTACA ATGAAATTGA CTTCCGCAAT GTGCAATACG ATTGGGGTCA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ubr-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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