msh-6 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	msh-6
Entrez Gene ID	171914
Full Name	MSH (MutS Homolog) family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_058762.4	NP_491163.1	MSH (MutS Homolog) family
NM_058762.5	NP_491163.1	DNA mismatch repair protein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	msh6	NP_878280.1
Drosophila melanogaster (fruit fly)	Msh6	NP_648755.1
Caenorhabditis elegans (roundworm)	msh-6	NP_491163.1
Saccharomyces cerevisiae (baker's yeast)	MSH6	NP_010382.3
Kluyveromyces lactis	KLLA0C17732g	XP_452992.1
Xenopus tropicalis (tropical clawed frog)	msh6	XP_002935421.2
Pan troglodytes (chimpanzee)	MSH6	XP_003309101.1
Mus musculus (house mouse)	Msh6	NP_034960.1
Eremothecium gossypii	AGOS_AGR116W	NP_986782.2
Canis lupus familiaris (dog)	MSH6	XP_531814.2
Gallus gallus (chicken)	MSH6	XP_419359.3
Schizosaccharomyces pombe (fission yeast)	msh6	NP_588344.1
Magnaporthe oryzae (rice blast fungus)	MGG_00704	XP_003718302.1
Homo sapiens (human)	MSH6	NP_000170.1
	MSH6	XP_001113749.1
Bos taurus (cattle)	MSH6	NP_001179666.1
Rattus norvegicus (Norway rat)	LOC100360342	XP_006239796.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003190	XP_312887.5
Neurospora crassa	NCU08135	XP_959678.2
Arabidopsis thaliana (thale cress)	MSH6	NP_192116.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MMR surveillance is carried out by a single Msh-2/Msh-6 heterodimer
Title: Mutation rates, spectra and hotspots in mismatch repair-deficient Caenorhabditis elegans.

The following msh-6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msh-6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337088	NM_058762.5 Latest version!	Caenorhabditis elegans DNA mismatch repair protein (msh-6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa337088	NM_058762.4	Caenorhabditis elegans MSH (MutS Homolog) family (msh-6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msh-6 ( NM_058762.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491163.1 Caenorhabditis elegans MSH (MutS Homolog) family (msh-6), partial mRNA.

Price & Availability↑

CloneID	OCa337088
Clone ID Related Accession (Same CDS sequence)	NM_058762.4 , NM_058762.5
Accession Version	NM_058762.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	MSH (MutS Homolog) family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_058762.3. COMPLETENESS: incomplete on both ends.

ATGTCGAAGC GACAAAGCTC ACTGATGTCT TTCTTCACCA AAACTCCGAA ATCTGAGAAG 
CCGGAGGAAG AAGTTAAAGA AAAAAGTGTT GAGGAGCCGA AATCACTGAA AAATGATACT 
CCGAAGATAT CAAATGATTC CGAAAAGAAG GTCTTGAAAC GATCCAACTC AAAAACCGTC 
AGCTCACCGG TAAAAACTCC AAGAAACGCG TCAAAACGTC CAAAAGTCGT CGTTTGTTCC 
TCTTCTGAAG GTGAAGATGA CGATGGAGAT GAGGATTTTG AGATGAAAGA AGCCGAAGAA 
CACGAATCAT CGGATGAATC GGAAGCTGAT GAGAATGCGA GTGACTGTGA AGTTGTTGAA 
TCGCCAGAGA GCACACCGCA GAGCACACCG AAACGAGGAG GAAAGAAGAA GATTTCGAAA 
CCTCTCCTCG CTGAAAACAC CCCAAAAAGC GTAAAAATGG CTGGAAAATC GAAAAAAGTG 
ATTCCCGACG GAGAAGCCGT GTCGATGGCC GGAGTTTTGG ACAAAATGGA TAAAATTATG 
GACGGAGAGG GTGAACGCAA GCGAATCGTC GAGAAAACCA CCGGAGCCAA GAACAAGGCA 
GTGGAGCTGG AGCCCGCTGA AAGATTTGAT CATGAATCTT TTGATTTTCT GAAGCCGGAT 
AAAATTCGTG ATGGTTTCAA GCGTCCGATG TCTGATCCAG AATATGATCC AAAAACTCTC 
TGGGTTCCTC CAGATTTTCA TCAAAAACAG ACACCAGGTC ATCGACAATG GTGGACTATG 
AAATCACAGC ATTTCGATAC AATTCTCCTT TTTAAAGTTG GAAAATTCTA TGAAACATAT 
CATATGGATG CAGTGGAAGT TGTTCGTGCA CTTAATATTG CTTTTATGCG TGGATCATAT 
GCTCATGCTG GCTTCCCGGA GCACGCAGCC TCAAAATTCG CTGATCAACT AATGAATCAC 
GGCTATAAAG TGGCTCGGAT CGAGCAGACT GAGACACCTC AAATGTTGGA GGAGAGAAAT 
CAGAAAACGA AAACAAAGGA AAAAGTTGTG AGGAGAGAAG TGTGTAGGGT TACATCGAAT 
GGAACACGAA CTTATGGAGT TTTGGATGGT GTTGATTTGG GAAGTGCTTC GTCGACGTTG 
GATCCGACTG CTAAGCATTT GTTGGCGATT AAGGAATTCC ATAACCCAGA AACAGGAAAA 
TCCTCCTACG GAGTGTGCAT GATTGACACA ACGACTGCAC ACATTCGTAT CGGACAATTT 
GAAGATGATG ACTATCGTTC ACAACTGCGT ACACTTCTCG CGAATGTGAT TGTTGTTCAG 
GCGATCGTTG AGCGTGGATC CATCTCTTCA ACCACTAAAT CAATTATTAA TGGAATTCTT 
TTCTCGGTTC CCGTTGAGCA TTTACTCCCG AAAAAGCAAT TTATGACAGC CGAAGATGTT 
GTGAGAATTG TTTCAAATGA AGATTATTAT GGATCTGATG CTTCTGAATG GCCGGAAGTT 
CTGAAGGGAA TGCTGGAGGA TTCCAGTATT CTTCCAAAAC CTTCAACCGA CTGGCAACTG 
GCTCTTTCAG CATTTGGAGC AATTTTCTGG TATCTTCGTG ACAGCCTAAT CGATGTGGAT 
ATGTTATCAA TGAGGAATGT GACTATTTAT AATTCGAATT CTATGGAAAA TGATCAGAAA 
AAGGAAAAGA TTGACTGGAA CGGAAAGAAT CTGATTCTCG ACGGAACTGC ACTGGAAAAT 
CTGAATATTG TACCAAATGG CAGAGATTCT CATCTCACTT CCCTCTACTA TGTCATTAAC 
AAATGCTCCA CACCATTTGG CCGTCGACTT CTCCGTTCGT GGCTTCTTCA ACCGACTTGT 
GATCCGAAAA AGCTCGAGCA ACGACAGAAA GCCATTAAGT GGCTCGTTTC ACCGGATGCC 
TCGTCATTTA TGACGACTGC GACGGCTACG TTGAAAAAGA TTCCAGATTT GGATAGACTT 
TTGCAAAAAA TCCACACAAT TGGCCTCAAA TACCGTTCCG AAAAGCATCC GGACAGTCGT 
GCAATCTTCT TCGATACAAT CAAAACAAAT CAGAAAAAAA TCGCCGAACT TTTGGCCGCA 
ATTGATGGAT TCAAACTGTG TAATAAACTG AGAAAAGAGT ATATAAAAGT ACAAAAAGAG 
GGTGAAGGAT GTGAATTACT TGATGAATTA CTTGGAAATG AGCAAGAAAT GGAAGAAGTC 
GATGAGAATA TCTACTTTTT CGAGAAAATG TTCGATCGAA GCACGGCTAT GAAAGATGGG 
AAAATTGTGC CGAATGCGGG ATGTGATGAG GAATATGATG AAGCTTTGAA TCGAGTGAAG 
GAAGCATTGA ATGAATTGAA TGATTATAAG GATTCTGTTG CCAAGAAATA TAGTTGCTCG 
ATCAAATTCG TGGATTCAGG AAAAGTAAAG TACCTACTGG AAATGCCTGA AAACACGAAA 
GTCTCATCGT CATTCGAGCT GAAATCTCGT CGAAAGGGCT TCATCCGATA CTCTACACCG 
GATTCTGAGC AACTGGTCGC CGCTCTTGAT GCAGTTGAAA AGGAAAAATC AAAATTGGGT 
GATGATGCGA CTCGTCGTGT ATTCGAACAA TTTGGACACA AAAATCCAAT TTGGTTGGAG 
ACTGTGAAGC TTGTCTCGAG CTTTGATGTG CTCACCTCAT TGGCTTTGTT CGCAAAATCT 
TCTCCATTCG AGATGTGTAT GCCGGAATTC GATTTCAACG CCACGGATCC TTATCTGATC 
GTTGACAAGG GTGTACATCC ATGTTTGGCG TTACAATCAA GAAATGAAGT TACCCAGACA 
ACATCGTTCA TTGCGAATAG TACCACAATG GGAGCATCTG AAGCTGCTGT GATGCTTCTG 
ACTGGTCCGA ATATGGGTGG AAAATCTACG TTGATGAGGC AAACCGCTGT TCTGGCGATT 
CTCGCTCATA TTGGTTCAAT GGTTCCTGCC TTCTCGATGC GGCTCACTCC AATCGATCGA 
ATTTTCACTC GTATTGGAGC CAACGATAGG ATTATGTGCG GAGAATCAAC ATTCTTCATT 
GAGCTAAAAG AAACTGATAT TATGCTCAAA AATGCGACAA AGCATTCTCT CTTGCTCGTC 
GACGAGCTCG GTCGTGGCAC AAGTACATTC GATGGAACTG CCATTGCTTC TGCTGTTTTA 
CAAAAGATTT CTGATGATTT GGCGTGTCGA ACATTCTTTT CTACACATTA TCACTCGATT 
TGTGACTCAT TTACTAATCA TCCGAATGTC AGATTGGCCC ATATGAAATG TGTCGTGGAT 
AAGGAGAACA ATGAGGATCC AACAATGGAA GATGTCACTT TCTTATACGA ACTCGAATCT 
GGAATTTGCC CAAAATCCTA CGGTTTCTAT GCAGCAAAAC TGGCTGGAAT TGACCATCAA 
GTCGTTCGAA ATGCCTATTT GGAAAGCAAT AAATTCGCAT CAAATCTCAT CATTGATCCC 
AAAATCCGAC ATCTTGTTGA ATGTGCTCGT GACGATAATT TTGATGTTGG AGAGTTGAAG 
CGAATGATTG AAGCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491163.1
CDS	1..3561
Translation	MSKRQSSLMSFFTKTPKSEKPEEEVKEKSVEEPKSLKNDTPKISNDSEKKVLKRSNSKTVSSPVKTPRNASKRPKVVVCSSSEGEDDDGDEDFEMKEAEEHESSDESEADENASDCEVVESPESTPQSTPKRGGKKKISKPLLAENTPKSVKMAGKSKKVIPDGEAVSMAGVLDKMDKIMDGEGERKRIVEKTTGAKNKAVELEPAERFDHESFDFLKPDKIRDGFKRPMSDPEYDPKTLWVPPDFHQKQTPGHRQWWTMKSQHFDTILLFKVGKFYETYHMDAVEVVRALNIAFMRGSYAHAGFPEHAASKFADQLMNHGYKVARIEQTETPQMLEERNQKTKTKEKVVRREVCRVTSNGTRTYGVLDGVDLGSASSTLDPTAKHLLAIKEFHNPETGKSSYGVCMIDTTTAHIRIGQFEDDDYRSQLRTLLANVIVVQAIVERGSISSTTKSIINGILFSVPVEHLLPKKQFMTAEDVVRIVSNEDYYGSDASEWPEVLKGMLEDSSILPKPSTDWQLALSAFGAIFWYLRDSLIDVDMLSMRNVTIYNSNSMENDQKKEKIDWNGKNLILDGTALENLNIVPNGRDSHLTSLYYVINKCSTPFGRRLLRSWLLQPTCDPKKLEQRQKAIKWLVSPDASSFMTTATATLKKIPDLDRLLQKIHTIGLKYRSEKHPDSRAIFFDTIKTNQKKIAELLAAIDGFKLCNKLRKEYIKVQKEGEGCELLDELLGNEQEMEEVDENIYFFEKMFDRSTAMKDGKIVPNAGCDEEYDEALNRVKEALNELNDYKDSVAKKYSCSIKFVDSGKVKYLLEMPENTKVSSSFELKSRRKGFIRYSTPDSEQLVAALDAVEKEKSKLGDDATRRVFEQFGHKNPIWLETVKLVSSFDVLTSLALFAKSSPFEMCMPEFDFNATDPYLIVDKGVHPCLALQSRNEVTQTTSFIANSTTMGASEAAVMLLTGPNMGGKSTLMRQTAVLAILAHIGSMVPAFSMRLTPIDRIFTRIGANDRIMCGESTFFIELKETDIMLKNATKHSLLLVDELGRGTSTFDGTAIASAVLQKISDDLACRTFFSTHYHSICDSFTNHPNVRLAHMKCVVDKENNEDPTMEDVTFLYELESGICPKSYGFYAAKLAGIDHQVVRNAYLESNKFASNLIIDPKIRHLVECARDDNFDVGELKRMIEAI

Target ORF information:

RefSeq Version	NM_058762.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans MSH (MutS Homolog) family (msh-6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058762.4

ORF Insert Sequence:

ATGTCGAAGC GACAAAGCTC ACTGATGTCT TTCTTCACCA AAACTCCGAA ATCTGAGAAG 
CCGGAGGAAG AAGTTAAAGA AAAAAGTGTT GAGGAGCCGA AATCACTGAA AAATGATACT 
CCGAAGATAT CAAATGATTC CGAAAAGAAG GTCTTGAAAC GATCCAACTC AAAAACCGTC 
AGCTCACCGG TAAAAACTCC AAGAAACGCG TCAAAACGTC CAAAAGTCGT CGTTTGTTCC 
TCTTCTGAAG GTGAAGATGA CGATGGAGAT GAGGATTTTG AGATGAAAGA AGCCGAAGAA 
CACGAATCAT CGGATGAATC GGAAGCTGAT GAGAATGCGA GTGACTGTGA AGTTGTTGAA 
TCGCCAGAGA GCACACCGCA GAGCACACCG AAACGAGGAG GAAAGAAGAA GATTTCGAAA 
CCTCTCCTCG CTGAAAACAC CCCAAAAAGC GTAAAAATGG CTGGAAAATC GAAAAAAGTG 
ATTCCCGACG GAGAAGCCGT GTCGATGGCC GGAGTTTTGG ACAAAATGGA TAAAATTATG 
GACGGAGAGG GTGAACGCAA GCGAATCGTC GAGAAAACCA CCGGAGCCAA GAACAAGGCA 
GTGGAGCTGG AGCCCGCTGA AAGATTTGAT CATGAATCTT TTGATTTTCT GAAGCCGGAT 
AAAATTCGTG ATGGTTTCAA GCGTCCGATG TCTGATCCAG AATATGATCC AAAAACTCTC 
TGGGTTCCTC CAGATTTTCA TCAAAAACAG ACACCAGGTC ATCGACAATG GTGGACTATG 
AAATCACAGC ATTTCGATAC AATTCTCCTT TTTAAAGTTG GAAAATTCTA TGAAACATAT 
CATATGGATG CAGTGGAAGT TGTTCGTGCA CTTAATATTG CTTTTATGCG TGGATCATAT 
GCTCATGCTG GCTTCCCGGA GCACGCAGCC TCAAAATTCG CTGATCAACT AATGAATCAC 
GGCTATAAAG TGGCTCGGAT CGAGCAGACT GAGACACCTC AAATGTTGGA GGAGAGAAAT 
CAGAAAACGA AAACAAAGGA AAAAGTTGTG AGGAGAGAAG TGTGTAGGGT TACATCGAAT 
GGAACACGAA CTTATGGAGT TTTGGATGGT GTTGATTTGG GAAGTGCTTC GTCGACGTTG 
GATCCGACTG CTAAGCATTT GTTGGCGATT AAGGAATTCC ATAACCCAGA AACAGGAAAA 
TCCTCCTACG GAGTGTGCAT GATTGACACA ACGACTGCAC ACATTCGTAT CGGACAATTT 
GAAGATGATG ACTATCGTTC ACAACTGCGT ACACTTCTCG CGAATGTGAT TGTTGTTCAG 
GCGATCGTTG AGCGTGGATC CATCTCTTCA ACCACTAAAT CAATTATTAA TGGAATTCTT 
TTCTCGGTTC CCGTTGAGCA TTTACTCCCG AAAAAGCAAT TTATGACAGC CGAAGATGTT 
GTGAGAATTG TTTCAAATGA AGATTATTAT GGATCTGATG CTTCTGAATG GCCGGAAGTT 
CTGAAGGGAA TGCTGGAGGA TTCCAGTATT CTTCCAAAAC CTTCAACCGA CTGGCAACTG 
GCTCTTTCAG CATTTGGAGC AATTTTCTGG TATCTTCGTG ACAGCCTAAT CGATGTGGAT 
ATGTTATCAA TGAGGAATGT GACTATTTAT AATTCGAATT CTATGGAAAA TGATCAGAAA 
AAGGAAAAGA TTGACTGGAA CGGAAAGAAT CTGATTCTCG ACGGAACTGC ACTGGAAAAT 
CTGAATATTG TACCAAATGG CAGAGATTCT CATCTCACTT CCCTCTACTA TGTCATTAAC 
AAATGCTCCA CACCATTTGG CCGTCGACTT CTCCGTTCGT GGCTTCTTCA ACCGACTTGT 
GATCCGAAAA AGCTCGAGCA ACGACAGAAA GCCATTAAGT GGCTCGTTTC ACCGGATGCC 
TCGTCATTTA TGACGACTGC GACGGCTACG TTGAAAAAGA TTCCAGATTT GGATAGACTT 
TTGCAAAAAA TCCACACAAT TGGCCTCAAA TACCGTTCCG AAAAGCATCC GGACAGTCGT 
GCAATCTTCT TCGATACAAT CAAAACAAAT CAGAAAAAAA TCGCCGAACT TTTGGCCGCA 
ATTGATGGAT TCAAACTGTG TAATAAACTG AGAAAAGAGT ATATAAAAGT ACAAAAAGAG 
GGTGAAGGAT GTGAATTACT TGATGAATTA CTTGGAAATG AGCAAGAAAT GGAAGAAGTC 
GATGAGAATA TCTACTTTTT CGAGAAAATG TTCGATCGAA GCACGGCTAT GAAAGATGGG 
AAAATTGTGC CGAATGCGGG ATGTGATGAG GAATATGATG AAGCTTTGAA TCGAGTGAAG 
GAAGCATTGA ATGAATTGAA TGATTATAAG GATTCTGTTG CCAAGAAATA TAGTTGCTCG 
ATCAAATTCG TGGATTCAGG AAAAGTAAAG TACCTACTGG AAATGCCTGA AAACACGAAA 
GTCTCATCGT CATTCGAGCT GAAATCTCGT CGAAAGGGCT TCATCCGATA CTCTACACCG 
GATTCTGAGC AACTGGTCGC CGCTCTTGAT GCAGTTGAAA AGGAAAAATC AAAATTGGGT 
GATGATGCGA CTCGTCGTGT ATTCGAACAA TTTGGACACA AAAATCCAAT TTGGTTGGAG 
ACTGTGAAGC TTGTCTCGAG CTTTGATGTG CTCACCTCAT TGGCTTTGTT CGCAAAATCT 
TCTCCATTCG AGATGTGTAT GCCGGAATTC GATTTCAACG CCACGGATCC TTATCTGATC 
GTTGACAAGG GTGTACATCC ATGTTTGGCG TTACAATCAA GAAATGAAGT TACCCAGACA 
ACATCGTTCA TTGCGAATAG TACCACAATG GGAGCATCTG AAGCTGCTGT GATGCTTCTG 
ACTGGTCCGA ATATGGGTGG AAAATCTACG TTGATGAGGC AAACCGCTGT TCTGGCGATT 
CTCGCTCATA TTGGTTCAAT GGTTCCTGCC TTCTCGATGC GGCTCACTCC AATCGATCGA 
ATTTTCACTC GTATTGGAGC CAACGATAGG ATTATGTGCG GAGAATCAAC ATTCTTCATT 
GAGCTAAAAG AAACTGATAT TATGCTCAAA AATGCGACAA AGCATTCTCT CTTGCTCGTC 
GACGAGCTCG GTCGTGGCAC AAGTACATTC GATGGAACTG CCATTGCTTC TGCTGTTTTA 
CAAAAGATTT CTGATGATTT GGCGTGTCGA ACATTCTTTT CTACACATTA TCACTCGATT 
TGTGACTCAT TTACTAATCA TCCGAATGTC AGATTGGCCC ATATGAAATG TGTCGTGGAT 
AAGGAGAACA ATGAGGATCC AACAATGGAA GATGTCACTT TCTTATACGA ACTCGAATCT 
GGAATTTGCC CAAAATCCTA CGGTTTCTAT GCAGCAAAAC TGGCTGGAAT TGACCATCAA 
GTCGTTCGAA ATGCCTATTT GGAAAGCAAT AAATTCGCAT CAAATCTCAT CATTGATCCC 
AAAATCCGAC ATCTTGTTGA ATGTGCTCGT GACGATAATT TTGATGTTGG AGAGTTGAAG 
CGAATGATTG AAGCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msh-6 ( NM_058762.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491163.1 Caenorhabditis elegans DNA mismatch repair protein (msh-6), mRNA.

Price & Availability↑

CloneID	OCa337088
Clone ID Related Accession (Same CDS sequence)	NM_058762.4 , NM_058762.5
Accession Version	NM_058762.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA mismatch repair protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058762.4.

ATGTCGAAGC GACAAAGCTC ACTGATGTCT TTCTTCACCA AAACTCCGAA ATCTGAGAAG 
CCGGAGGAAG AAGTTAAAGA AAAAAGTGTT GAGGAGCCGA AATCACTGAA AAATGATACT 
CCGAAGATAT CAAATGATTC CGAAAAGAAG GTCTTGAAAC GATCCAACTC AAAAACCGTC 
AGCTCACCGG TAAAAACTCC AAGAAACGCG TCAAAACGTC CAAAAGTCGT CGTTTGTTCC 
TCTTCTGAAG GTGAAGATGA CGATGGAGAT GAGGATTTTG AGATGAAAGA AGCCGAAGAA 
CACGAATCAT CGGATGAATC GGAAGCTGAT GAGAATGCGA GTGACTGTGA AGTTGTTGAA 
TCGCCAGAGA GCACACCGCA GAGCACACCG AAACGAGGAG GAAAGAAGAA GATTTCGAAA 
CCTCTCCTCG CTGAAAACAC CCCAAAAAGC GTAAAAATGG CTGGAAAATC GAAAAAAGTG 
ATTCCCGACG GAGAAGCCGT GTCGATGGCC GGAGTTTTGG ACAAAATGGA TAAAATTATG 
GACGGAGAGG GTGAACGCAA GCGAATCGTC GAGAAAACCA CCGGAGCCAA GAACAAGGCA 
GTGGAGCTGG AGCCCGCTGA AAGATTTGAT CATGAATCTT TTGATTTTCT GAAGCCGGAT 
AAAATTCGTG ATGGTTTCAA GCGTCCGATG TCTGATCCAG AATATGATCC AAAAACTCTC 
TGGGTTCCTC CAGATTTTCA TCAAAAACAG ACACCAGGTC ATCGACAATG GTGGACTATG 
AAATCACAGC ATTTCGATAC AATTCTCCTT TTTAAAGTTG GAAAATTCTA TGAAACATAT 
CATATGGATG CAGTGGAAGT TGTTCGTGCA CTTAATATTG CTTTTATGCG TGGATCATAT 
GCTCATGCTG GCTTCCCGGA GCACGCAGCC TCAAAATTCG CTGATCAACT AATGAATCAC 
GGCTATAAAG TGGCTCGGAT CGAGCAGACT GAGACACCTC AAATGTTGGA GGAGAGAAAT 
CAGAAAACGA AAACAAAGGA AAAAGTTGTG AGGAGAGAAG TGTGTAGGGT TACATCGAAT 
GGAACACGAA CTTATGGAGT TTTGGATGGT GTTGATTTGG GAAGTGCTTC GTCGACGTTG 
GATCCGACTG CTAAGCATTT GTTGGCGATT AAGGAATTCC ATAACCCAGA AACAGGAAAA 
TCCTCCTACG GAGTGTGCAT GATTGACACA ACGACTGCAC ACATTCGTAT CGGACAATTT 
GAAGATGATG ACTATCGTTC ACAACTGCGT ACACTTCTCG CGAATGTGAT TGTTGTTCAG 
GCGATCGTTG AGCGTGGATC CATCTCTTCA ACCACTAAAT CAATTATTAA TGGAATTCTT 
TTCTCGGTTC CCGTTGAGCA TTTACTCCCG AAAAAGCAAT TTATGACAGC CGAAGATGTT 
GTGAGAATTG TTTCAAATGA AGATTATTAT GGATCTGATG CTTCTGAATG GCCGGAAGTT 
CTGAAGGGAA TGCTGGAGGA TTCCAGTATT CTTCCAAAAC CTTCAACCGA CTGGCAACTG 
GCTCTTTCAG CATTTGGAGC AATTTTCTGG TATCTTCGTG ACAGCCTAAT CGATGTGGAT 
ATGTTATCAA TGAGGAATGT GACTATTTAT AATTCGAATT CTATGGAAAA TGATCAGAAA 
AAGGAAAAGA TTGACTGGAA CGGAAAGAAT CTGATTCTCG ACGGAACTGC ACTGGAAAAT 
CTGAATATTG TACCAAATGG CAGAGATTCT CATCTCACTT CCCTCTACTA TGTCATTAAC 
AAATGCTCCA CACCATTTGG CCGTCGACTT CTCCGTTCGT GGCTTCTTCA ACCGACTTGT 
GATCCGAAAA AGCTCGAGCA ACGACAGAAA GCCATTAAGT GGCTCGTTTC ACCGGATGCC 
TCGTCATTTA TGACGACTGC GACGGCTACG TTGAAAAAGA TTCCAGATTT GGATAGACTT 
TTGCAAAAAA TCCACACAAT TGGCCTCAAA TACCGTTCCG AAAAGCATCC GGACAGTCGT 
GCAATCTTCT TCGATACAAT CAAAACAAAT CAGAAAAAAA TCGCCGAACT TTTGGCCGCA 
ATTGATGGAT TCAAACTGTG TAATAAACTG AGAAAAGAGT ATATAAAAGT ACAAAAAGAG 
GGTGAAGGAT GTGAATTACT TGATGAATTA CTTGGAAATG AGCAAGAAAT GGAAGAAGTC 
GATGAGAATA TCTACTTTTT CGAGAAAATG TTCGATCGAA GCACGGCTAT GAAAGATGGG 
AAAATTGTGC CGAATGCGGG ATGTGATGAG GAATATGATG AAGCTTTGAA TCGAGTGAAG 
GAAGCATTGA ATGAATTGAA TGATTATAAG GATTCTGTTG CCAAGAAATA TAGTTGCTCG 
ATCAAATTCG TGGATTCAGG AAAAGTAAAG TACCTACTGG AAATGCCTGA AAACACGAAA 
GTCTCATCGT CATTCGAGCT GAAATCTCGT CGAAAGGGCT TCATCCGATA CTCTACACCG 
GATTCTGAGC AACTGGTCGC CGCTCTTGAT GCAGTTGAAA AGGAAAAATC AAAATTGGGT 
GATGATGCGA CTCGTCGTGT ATTCGAACAA TTTGGACACA AAAATCCAAT TTGGTTGGAG 
ACTGTGAAGC TTGTCTCGAG CTTTGATGTG CTCACCTCAT TGGCTTTGTT CGCAAAATCT 
TCTCCATTCG AGATGTGTAT GCCGGAATTC GATTTCAACG CCACGGATCC TTATCTGATC 
GTTGACAAGG GTGTACATCC ATGTTTGGCG TTACAATCAA GAAATGAAGT TACCCAGACA 
ACATCGTTCA TTGCGAATAG TACCACAATG GGAGCATCTG AAGCTGCTGT GATGCTTCTG 
ACTGGTCCGA ATATGGGTGG AAAATCTACG TTGATGAGGC AAACCGCTGT TCTGGCGATT 
CTCGCTCATA TTGGTTCAAT GGTTCCTGCC TTCTCGATGC GGCTCACTCC AATCGATCGA 
ATTTTCACTC GTATTGGAGC CAACGATAGG ATTATGTGCG GAGAATCAAC ATTCTTCATT 
GAGCTAAAAG AAACTGATAT TATGCTCAAA AATGCGACAA AGCATTCTCT CTTGCTCGTC 
GACGAGCTCG GTCGTGGCAC AAGTACATTC GATGGAACTG CCATTGCTTC TGCTGTTTTA 
CAAAAGATTT CTGATGATTT GGCGTGTCGA ACATTCTTTT CTACACATTA TCACTCGATT 
TGTGACTCAT TTACTAATCA TCCGAATGTC AGATTGGCCC ATATGAAATG TGTCGTGGAT 
AAGGAGAACA ATGAGGATCC AACAATGGAA GATGTCACTT TCTTATACGA ACTCGAATCT 
GGAATTTGCC CAAAATCCTA CGGTTTCTAT GCAGCAAAAC TGGCTGGAAT TGACCATCAA 
GTCGTTCGAA ATGCCTATTT GGAAAGCAAT AAATTCGCAT CAAATCTCAT CATTGATCCC 
AAAATCCGAC ATCTTGTTGA ATGTGCTCGT GACGATAATT TTGATGTTGG AGAGTTGAAG 
CGAATGATTG AAGCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491163.1
CDS	2..3562
Translation	MSKRQSSLMSFFTKTPKSEKPEEEVKEKSVEEPKSLKNDTPKISNDSEKKVLKRSNSKTVSSPVKTPRNASKRPKVVVCSSSEGEDDDGDEDFEMKEAEEHESSDESEADENASDCEVVESPESTPQSTPKRGGKKKISKPLLAENTPKSVKMAGKSKKVIPDGEAVSMAGVLDKMDKIMDGEGERKRIVEKTTGAKNKAVELEPAERFDHESFDFLKPDKIRDGFKRPMSDPEYDPKTLWVPPDFHQKQTPGHRQWWTMKSQHFDTILLFKVGKFYETYHMDAVEVVRALNIAFMRGSYAHAGFPEHAASKFADQLMNHGYKVARIEQTETPQMLEERNQKTKTKEKVVRREVCRVTSNGTRTYGVLDGVDLGSASSTLDPTAKHLLAIKEFHNPETGKSSYGVCMIDTTTAHIRIGQFEDDDYRSQLRTLLANVIVVQAIVERGSISSTTKSIINGILFSVPVEHLLPKKQFMTAEDVVRIVSNEDYYGSDASEWPEVLKGMLEDSSILPKPSTDWQLALSAFGAIFWYLRDSLIDVDMLSMRNVTIYNSNSMENDQKKEKIDWNGKNLILDGTALENLNIVPNGRDSHLTSLYYVINKCSTPFGRRLLRSWLLQPTCDPKKLEQRQKAIKWLVSPDASSFMTTATATLKKIPDLDRLLQKIHTIGLKYRSEKHPDSRAIFFDTIKTNQKKIAELLAAIDGFKLCNKLRKEYIKVQKEGEGCELLDELLGNEQEMEEVDENIYFFEKMFDRSTAMKDGKIVPNAGCDEEYDEALNRVKEALNELNDYKDSVAKKYSCSIKFVDSGKVKYLLEMPENTKVSSSFELKSRRKGFIRYSTPDSEQLVAALDAVEKEKSKLGDDATRRVFEQFGHKNPIWLETVKLVSSFDVLTSLALFAKSSPFEMCMPEFDFNATDPYLIVDKGVHPCLALQSRNEVTQTTSFIANSTTMGASEAAVMLLTGPNMGGKSTLMRQTAVLAILAHIGSMVPAFSMRLTPIDRIFTRIGANDRIMCGESTFFIELKETDIMLKNATKHSLLLVDELGRGTSTFDGTAIASAVLQKISDDLACRTFFSTHYHSICDSFTNHPNVRLAHMKCVVDKENNEDPTMEDVTFLYELESGICPKSYGFYAAKLAGIDHQVVRNAYLESNKFASNLIIDPKIRHLVECARDDNFDVGELKRMIEAI

Target ORF information:

RefSeq Version	NM_058762.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA mismatch repair protein (msh-6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058762.5

ORF Insert Sequence:

ATGTCGAAGC GACAAAGCTC ACTGATGTCT TTCTTCACCA AAACTCCGAA ATCTGAGAAG 
CCGGAGGAAG AAGTTAAAGA AAAAAGTGTT GAGGAGCCGA AATCACTGAA AAATGATACT 
CCGAAGATAT CAAATGATTC CGAAAAGAAG GTCTTGAAAC GATCCAACTC AAAAACCGTC 
AGCTCACCGG TAAAAACTCC AAGAAACGCG TCAAAACGTC CAAAAGTCGT CGTTTGTTCC 
TCTTCTGAAG GTGAAGATGA CGATGGAGAT GAGGATTTTG AGATGAAAGA AGCCGAAGAA 
CACGAATCAT CGGATGAATC GGAAGCTGAT GAGAATGCGA GTGACTGTGA AGTTGTTGAA 
TCGCCAGAGA GCACACCGCA GAGCACACCG AAACGAGGAG GAAAGAAGAA GATTTCGAAA 
CCTCTCCTCG CTGAAAACAC CCCAAAAAGC GTAAAAATGG CTGGAAAATC GAAAAAAGTG 
ATTCCCGACG GAGAAGCCGT GTCGATGGCC GGAGTTTTGG ACAAAATGGA TAAAATTATG 
GACGGAGAGG GTGAACGCAA GCGAATCGTC GAGAAAACCA CCGGAGCCAA GAACAAGGCA 
GTGGAGCTGG AGCCCGCTGA AAGATTTGAT CATGAATCTT TTGATTTTCT GAAGCCGGAT 
AAAATTCGTG ATGGTTTCAA GCGTCCGATG TCTGATCCAG AATATGATCC AAAAACTCTC 
TGGGTTCCTC CAGATTTTCA TCAAAAACAG ACACCAGGTC ATCGACAATG GTGGACTATG 
AAATCACAGC ATTTCGATAC AATTCTCCTT TTTAAAGTTG GAAAATTCTA TGAAACATAT 
CATATGGATG CAGTGGAAGT TGTTCGTGCA CTTAATATTG CTTTTATGCG TGGATCATAT 
GCTCATGCTG GCTTCCCGGA GCACGCAGCC TCAAAATTCG CTGATCAACT AATGAATCAC 
GGCTATAAAG TGGCTCGGAT CGAGCAGACT GAGACACCTC AAATGTTGGA GGAGAGAAAT 
CAGAAAACGA AAACAAAGGA AAAAGTTGTG AGGAGAGAAG TGTGTAGGGT TACATCGAAT 
GGAACACGAA CTTATGGAGT TTTGGATGGT GTTGATTTGG GAAGTGCTTC GTCGACGTTG 
GATCCGACTG CTAAGCATTT GTTGGCGATT AAGGAATTCC ATAACCCAGA AACAGGAAAA 
TCCTCCTACG GAGTGTGCAT GATTGACACA ACGACTGCAC ACATTCGTAT CGGACAATTT 
GAAGATGATG ACTATCGTTC ACAACTGCGT ACACTTCTCG CGAATGTGAT TGTTGTTCAG 
GCGATCGTTG AGCGTGGATC CATCTCTTCA ACCACTAAAT CAATTATTAA TGGAATTCTT 
TTCTCGGTTC CCGTTGAGCA TTTACTCCCG AAAAAGCAAT TTATGACAGC CGAAGATGTT 
GTGAGAATTG TTTCAAATGA AGATTATTAT GGATCTGATG CTTCTGAATG GCCGGAAGTT 
CTGAAGGGAA TGCTGGAGGA TTCCAGTATT CTTCCAAAAC CTTCAACCGA CTGGCAACTG 
GCTCTTTCAG CATTTGGAGC AATTTTCTGG TATCTTCGTG ACAGCCTAAT CGATGTGGAT 
ATGTTATCAA TGAGGAATGT GACTATTTAT AATTCGAATT CTATGGAAAA TGATCAGAAA 
AAGGAAAAGA TTGACTGGAA CGGAAAGAAT CTGATTCTCG ACGGAACTGC ACTGGAAAAT 
CTGAATATTG TACCAAATGG CAGAGATTCT CATCTCACTT CCCTCTACTA TGTCATTAAC 
AAATGCTCCA CACCATTTGG CCGTCGACTT CTCCGTTCGT GGCTTCTTCA ACCGACTTGT 
GATCCGAAAA AGCTCGAGCA ACGACAGAAA GCCATTAAGT GGCTCGTTTC ACCGGATGCC 
TCGTCATTTA TGACGACTGC GACGGCTACG TTGAAAAAGA TTCCAGATTT GGATAGACTT 
TTGCAAAAAA TCCACACAAT TGGCCTCAAA TACCGTTCCG AAAAGCATCC GGACAGTCGT 
GCAATCTTCT TCGATACAAT CAAAACAAAT CAGAAAAAAA TCGCCGAACT TTTGGCCGCA 
ATTGATGGAT TCAAACTGTG TAATAAACTG AGAAAAGAGT ATATAAAAGT ACAAAAAGAG 
GGTGAAGGAT GTGAATTACT TGATGAATTA CTTGGAAATG AGCAAGAAAT GGAAGAAGTC 
GATGAGAATA TCTACTTTTT CGAGAAAATG TTCGATCGAA GCACGGCTAT GAAAGATGGG 
AAAATTGTGC CGAATGCGGG ATGTGATGAG GAATATGATG AAGCTTTGAA TCGAGTGAAG 
GAAGCATTGA ATGAATTGAA TGATTATAAG GATTCTGTTG CCAAGAAATA TAGTTGCTCG 
ATCAAATTCG TGGATTCAGG AAAAGTAAAG TACCTACTGG AAATGCCTGA AAACACGAAA 
GTCTCATCGT CATTCGAGCT GAAATCTCGT CGAAAGGGCT TCATCCGATA CTCTACACCG 
GATTCTGAGC AACTGGTCGC CGCTCTTGAT GCAGTTGAAA AGGAAAAATC AAAATTGGGT 
GATGATGCGA CTCGTCGTGT ATTCGAACAA TTTGGACACA AAAATCCAAT TTGGTTGGAG 
ACTGTGAAGC TTGTCTCGAG CTTTGATGTG CTCACCTCAT TGGCTTTGTT CGCAAAATCT 
TCTCCATTCG AGATGTGTAT GCCGGAATTC GATTTCAACG CCACGGATCC TTATCTGATC 
GTTGACAAGG GTGTACATCC ATGTTTGGCG TTACAATCAA GAAATGAAGT TACCCAGACA 
ACATCGTTCA TTGCGAATAG TACCACAATG GGAGCATCTG AAGCTGCTGT GATGCTTCTG 
ACTGGTCCGA ATATGGGTGG AAAATCTACG TTGATGAGGC AAACCGCTGT TCTGGCGATT 
CTCGCTCATA TTGGTTCAAT GGTTCCTGCC TTCTCGATGC GGCTCACTCC AATCGATCGA 
ATTTTCACTC GTATTGGAGC CAACGATAGG ATTATGTGCG GAGAATCAAC ATTCTTCATT 
GAGCTAAAAG AAACTGATAT TATGCTCAAA AATGCGACAA AGCATTCTCT CTTGCTCGTC 
GACGAGCTCG GTCGTGGCAC AAGTACATTC GATGGAACTG CCATTGCTTC TGCTGTTTTA 
CAAAAGATTT CTGATGATTT GGCGTGTCGA ACATTCTTTT CTACACATTA TCACTCGATT 
TGTGACTCAT TTACTAATCA TCCGAATGTC AGATTGGCCC ATATGAAATG TGTCGTGGAT 
AAGGAGAACA ATGAGGATCC AACAATGGAA GATGTCACTT TCTTATACGA ACTCGAATCT 
GGAATTTGCC CAAAATCCTA CGGTTTCTAT GCAGCAAAAC TGGCTGGAAT TGACCATCAA 
GTCGTTCGAA ATGCCTATTT GGAAAGCAAT AAATTCGCAT CAAATCTCAT CATTGATCCC 
AAAATCCGAC ATCTTGTTGA ATGTGCTCGT GACGATAATT TTGATGTTGG AGAGTTGAAG 
CGAATGATTG AAGCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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msh-6 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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