smg-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	smg-2
Entrez Gene ID	171696
Full Name	Regulator of nonsense transcripts 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_058428.4	NP_490829.1	Regulator of nonsense transcripts 1
NM_058428.5	NP_490829.1	Regulator of nonsense transcripts 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UPF1	XP_512972.4
Mus musculus (house mouse)	Upf1	NP_001116301.1
Eremothecium gossypii	AGOS_ABR022C	NP_982968.4
	LOC720288	XP_002808229.1
Canis lupus familiaris (dog)	UPF1	XP_005632737.1
Bos taurus (cattle)	UPF1	XP_005208537.1
Saccharomyces cerevisiae (baker's yeast)	NAM7	NP_013797.1
Schizosaccharomyces pombe (fission yeast)	upf1	NP_593080.1
Magnaporthe oryzae (rice blast fungus)	MGG_00976	XP_003717973.1
Gallus gallus (chicken)	UPF1	XP_418237.3
Danio rerio (zebrafish)	upf1	NP_998639.1
Drosophila melanogaster (fruit fly)	Upf1	NP_572767.1
Oryza sativa (rice)	Os07g0495900	NP_001059698.1
Homo sapiens (human)	UPF1	NP_002902.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001133	XP_322028.4
Caenorhabditis elegans (roundworm)	smg-2	NP_490829.1
Kluyveromyces lactis	KLLA0B06435g	XP_451821.1
Neurospora crassa	NCU04242	XP_961233.1
Rattus norvegicus (Norway rat)	Upf1	XP_003751614.1
Arabidopsis thaliana (thale cress)	LBA1	NP_199512.2
Xenopus tropicalis (tropical clawed frog)	upf1	XP_002938872.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SMG-2 interacts with SMG-3, SMG-3 interacts with SMG-4, and SMG-2 interacts indirectly with SMG-4 via shared interactions with SMG-3. SMG-2 preferentially associates with PTC-containing mRNAs.
Title: Caenorhabditis elegans SMG-2 selectively marks mRNAs containing premature translation termination codons.

results suggest that the role of SMG-5 is to direct protein phosphatase 2A to its SMG-2 substrate
Title: SMG-5, required for C.elegans nonsense-mediated mRNA decay, associates with SMG-2 and protein phosphatase 2A.

The following smg-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337176	NM_058428.5 Latest version!	Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa337176	NM_058428.4	Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg-2 ( NM_058428.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_490829.1 Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), partial mRNA.

Price & Availability↑

CloneID	OCa337176
Clone ID Related Accession (Same CDS sequence)	NM_058428.4 , NM_058428.5
Accession Version	NM_058428.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Regulator of nonsense transcripts 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_058428.3. COMPLETENESS: incomplete on both ends.

ATGGACGATT CGGACGACGA ATATTCGAGA AGCCACGGGG AAACCCTCAC ATTTGTGGAT 
CCCGAGGACG ATGGAGTCTC TATTGGGAAC ACGCAGGACT CTCAGTTCGC CTATGAGCAG 
TTTAGTGTGC CTACGCAGAG TTCTCAGGCG ACAGATCTGC TGCCAGGAGG AACCGATGGA 
ACCACCAACG ATTTGCCATT TCACGACGTG GAAGACGACG AGTCGGACAG TGAAAAATCA 
CTGACAGAAG AGCAGCAGCA GCAGAAGCTG CCGGAGCACG CGTGTCGCTA TTGTGGAATC 
TCTGATCCTC TGTGTGTCGC CAAATGCACA GTCTGCAGGA AATGGTTCTG TAATTCCAAT 
GATGGAACGT CGGGCGGGCA TATTGTTCAT CATATGGTTC GGAGTCAGCA TAAGGAAGCC 
TACACGCATA AGGATTCACC GTGTGGTGAC ACTCAATTGG AATGCTATCG TTGTGGTTCG 
AAAAACGTGT TTAATCTCGG CTTCATCCCC GGAAAAAAGG ATCAAGTCGT CGTGATAATC 
TGTAGAACAC CGTGCGCCAG TATTGCATTC CAGAATGATG ATAATTGGTC GCCCGAGGAC 
TGGAAATCTG TAATCGCTGA AAAACAGCTG CTATCGTGGA TTGTTAATGT GCCCAGCGAG 
GAGCAGGTGG CCAGAGCTAG GAAAATCACT GCTACCCAAG CAGTCCGAAT GGAAGAGCTC 
TGGCGTGATC ACCCAGAAGC AACCGTTGAC GATTTAAATA AACCGGGACT CGATCGAGAA 
CCGGATCATG TTCAATTAAG ATATGTGGAT GCTCATCACT ATTCGAAAGT GTTCCGTCCA 
TTAGTTGCAA TTGAAGCAGA ATATGATCGA AGAGTTAAAG AATCAGCATC ACAAGCAGTC 
GGAACTGTTA GATGGGAACA AGGACTTCGT CAATCGGTTC TCGCGTTCTT TCATCTTCCA 
CAATTTGCTG ATGGTGTCAT GAAGCTTGCG AAAGGCGATG AACTTCGATT GAAACATTCT 
CAGACGGTTG ATGGATCGGA ATGGACCAAG ATTGGAAGTG TTTTCAAGAT TCCTGATAAC 
CACGGCGACG AGGTAGGCAT CGAAATCCGC GGAGCTGTCG ACAAATCAGT GATGGAGAGT 
CGGATTATGT TCACCGTCGA CGTCGTCTGG AACGCCACCA CCTTCGAGCG ACAGTACAAG 
GCTCTTGCGG CGCTGCTCAA CGACTCCAAA GCAATATCTC CATATCTCTA CCAGAAGCTT 
CTGGGTCATC CAGCCGAGGA AATGATGCTC AAGTTTGATC TACCGCGTCG GCTATCTGTC 
GCTGGACTCC CCGAGCTTAA TAGCTCCCAG ATGCAGGCAG TGAAGCAGGT GCTCACCCGA 
CCTTTAAGCT TGATTCAAGG ACCTCCGGGA ACTGGAAAAA CCGTAGTTTC GGCGACAATC 
GTCTATCATT TGGTGCAGAA AACCGAGGGA AACGTGCTCG TGTGCTCGCC GAGCAACATC 
GCCGTCGATC ACTTGGCCGA GAAGATTCAC AAGACGGGAT TGAAGGTTGT GAGGCTTTGT 
GCGAGATCTC GAGAGCACAG TGAGACTACA GTACCCTACT TGACGCTTCA ACATCAGCTA 
AAAGTGATGG GCGGTGCGGA GCTTCAGAAG CTGATTCAGT TGAAAGATGA AGCCGGGGAG 
CTCGAGTTTA AGGATGATTT GAGATATATG CAATTGAAAA GAGTCAAAGA GCATGAGCTT 
CTCGCCGCCG CTGACGTCAT CTGTTGTACG TGCTCATCGG CGGCTGATGC GAGATTGTCG 
AAGATCCGTA CGAGAACTGT GCTCATTGAT GAGAGCACAC AGGCGACAGA GCCCGAGATT 
TTGGTCTCTA TTATGAGAGG AGTCAGGCAA TTGGTTTTAG TTGGAGATCA CTGTCAACTG 
GGACCGGTTG TTATATGTAA AAAGGCGGCG ATTGCCGGAT TGAGCCAATC TTTGTTCGAG 
AGACTTGTGC TTCTCGGTAT TCGACCGTTC CGGCTTCAGG TGCAATACCG TATGCATCCA 
GTGCTCTCAG AGTTCCCGAG CAACGTGTTT TACGATGGAA GTCTCCAGAA TGGTGTCACT 
GAAAACGATC GCCATATGAC AGGAGTCGAT TGGCATTGGC CTAAACCAAA TAAGCCGGCC 
TTCTTCTGGC ATTGCAGTGG ATCCGAAGAG CTTTCAGCAT CTGGAACCTC ATTTCTCAAT 
AGAACAGAAG CCGCGAATGT GGAGAAACTT GTGTCGAAAT TGATAAAAGC AGGCGTTCAA 
CCACATCAAA TCGGTGTTAT TACTCCGTAT GAGGGACAAC GTAGTTTTAT AGTCAATTAT 
ATGCATACAC AAGGAACTCT CAACTCGAAA CTCTATGAAA ATGTCGAAAT TGCTAGTGTT 
GACGCTTTTC AGGGTCGCGA AAAAGACTAT ATCATCGTAA CGTGTGTTCG ATCGAACGAT 
ATTCTCGGAA TCGGATTTCT CAGTGATCCA CGTCGTCTCA ACGTTGCAAT TACACGTGCC 
AAATACGGTT TGGTTCTAGT CGGAAATGCA AAGGTCTTGG CACGACATGA TCTATGGCAT 
GAGCTGATTA ATCACTATAA ATCGAAGGAA ATGCTCTACG AGGGACCGAT TAACGCGCTG 
AAACCGCTGA ATTTGGCATT GCCAAAGGCG ACGATTCGCA CCAAGAATAA TATAGCGGGC 
AACGCAAATC GATTCGGAAT CAAACGTATG CAATATACAT TCAATGAATA CAAATCCAAT 
GATCCATCAC AACCACGTCT TCCACCAACA TATTCAAACT CACAGAATCT GTTGAGTATG 
AGCAAGTTGG CTCAGACTTT TAACAAGAAT GTGCCCATCC CAGCACATAT GATGGATCCG 
AATGTCTATG CGGCCGCTAG AAATCAGAAA GACCGCCGCC GTGGTGACCA ACGCCGTCCT 
CCTCCACAAG CTGAAGCAGC AATGGATTTA TCGCAAGGAA TGATGTCTCA ACAATCTCAA 
CAATATCCAC CACAAGGAGC TTCTTCTCAA TCACAATATC TTCTCGACGG AGCTTCATCA 
CTATCCGGAT GGTCTCAATC TCAGACCACG ACCACCACCA CGCGTCACCA CCACCACCGT 
CAAAACCGCA ACTCTCAGCA GCAAATGTCT CAGGATATGG ACGATATTCA GCAGAAAATG 
GACGATTTGC TCTTTTCGCA GGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_490829.1
CDS	1..3210
Translation	MDDSDDEYSRSHGETLTFVDPEDDGVSIGNTQDSQFAYEQFSVPTQSSQATDLLPGGTDGTTNDLPFHDVEDDESDSEKSLTEEQQQQKLPEHACRYCGISDPLCVAKCTVCRKWFCNSNDGTSGGHIVHHMVRSQHKEAYTHKDSPCGDTQLECYRCGSKNVFNLGFIPGKKDQVVVIICRTPCASIAFQNDDNWSPEDWKSVIAEKQLLSWIVNVPSEEQVARARKITATQAVRMEELWRDHPEATVDDLNKPGLDREPDHVQLRYVDAHHYSKVFRPLVAIEAEYDRRVKESASQAVGTVRWEQGLRQSVLAFFHLPQFADGVMKLAKGDELRLKHSQTVDGSEWTKIGSVFKIPDNHGDEVGIEIRGAVDKSVMESRIMFTVDVVWNATTFERQYKALAALLNDSKAISPYLYQKLLGHPAEEMMLKFDLPRRLSVAGLPELNSSQMQAVKQVLTRPLSLIQGPPGTGKTVVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVRQLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRPFRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHCSGSEELSASGTSFLNRTEAANVEKLVSKLIKAGVQPHQIGVITPYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILGIGFLSDPRRLNVAITRAKYGLVLVGNAKVLARHDLWHELINHYKSKEMLYEGPINALKPLNLALPKATIRTKNNIAGNANRFGIKRMQYTFNEYKSNDPSQPRLPPTYSNSQNLLSMSKLAQTFNKNVPIPAHMMDPNVYAAARNQKDRRRGDQRRPPPQAEAAMDLSQGMMSQQSQQYPPQGASSQSQYLLDGASSLSGWSQSQTTTTTTRHHHHRQNRNSQQQMSQDMDDIQQKMDDLLFSQDC

Target ORF information:

RefSeq Version	NM_058428.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058428.4

ORF Insert Sequence:

ATGGACGATT CGGACGACGA ATATTCGAGA AGCCACGGGG AAACCCTCAC ATTTGTGGAT 
CCCGAGGACG ATGGAGTCTC TATTGGGAAC ACGCAGGACT CTCAGTTCGC CTATGAGCAG 
TTTAGTGTGC CTACGCAGAG TTCTCAGGCG ACAGATCTGC TGCCAGGAGG AACCGATGGA 
ACCACCAACG ATTTGCCATT TCACGACGTG GAAGACGACG AGTCGGACAG TGAAAAATCA 
CTGACAGAAG AGCAGCAGCA GCAGAAGCTG CCGGAGCACG CGTGTCGCTA TTGTGGAATC 
TCTGATCCTC TGTGTGTCGC CAAATGCACA GTCTGCAGGA AATGGTTCTG TAATTCCAAT 
GATGGAACGT CGGGCGGGCA TATTGTTCAT CATATGGTTC GGAGTCAGCA TAAGGAAGCC 
TACACGCATA AGGATTCACC GTGTGGTGAC ACTCAATTGG AATGCTATCG TTGTGGTTCG 
AAAAACGTGT TTAATCTCGG CTTCATCCCC GGAAAAAAGG ATCAAGTCGT CGTGATAATC 
TGTAGAACAC CGTGCGCCAG TATTGCATTC CAGAATGATG ATAATTGGTC GCCCGAGGAC 
TGGAAATCTG TAATCGCTGA AAAACAGCTG CTATCGTGGA TTGTTAATGT GCCCAGCGAG 
GAGCAGGTGG CCAGAGCTAG GAAAATCACT GCTACCCAAG CAGTCCGAAT GGAAGAGCTC 
TGGCGTGATC ACCCAGAAGC AACCGTTGAC GATTTAAATA AACCGGGACT CGATCGAGAA 
CCGGATCATG TTCAATTAAG ATATGTGGAT GCTCATCACT ATTCGAAAGT GTTCCGTCCA 
TTAGTTGCAA TTGAAGCAGA ATATGATCGA AGAGTTAAAG AATCAGCATC ACAAGCAGTC 
GGAACTGTTA GATGGGAACA AGGACTTCGT CAATCGGTTC TCGCGTTCTT TCATCTTCCA 
CAATTTGCTG ATGGTGTCAT GAAGCTTGCG AAAGGCGATG AACTTCGATT GAAACATTCT 
CAGACGGTTG ATGGATCGGA ATGGACCAAG ATTGGAAGTG TTTTCAAGAT TCCTGATAAC 
CACGGCGACG AGGTAGGCAT CGAAATCCGC GGAGCTGTCG ACAAATCAGT GATGGAGAGT 
CGGATTATGT TCACCGTCGA CGTCGTCTGG AACGCCACCA CCTTCGAGCG ACAGTACAAG 
GCTCTTGCGG CGCTGCTCAA CGACTCCAAA GCAATATCTC CATATCTCTA CCAGAAGCTT 
CTGGGTCATC CAGCCGAGGA AATGATGCTC AAGTTTGATC TACCGCGTCG GCTATCTGTC 
GCTGGACTCC CCGAGCTTAA TAGCTCCCAG ATGCAGGCAG TGAAGCAGGT GCTCACCCGA 
CCTTTAAGCT TGATTCAAGG ACCTCCGGGA ACTGGAAAAA CCGTAGTTTC GGCGACAATC 
GTCTATCATT TGGTGCAGAA AACCGAGGGA AACGTGCTCG TGTGCTCGCC GAGCAACATC 
GCCGTCGATC ACTTGGCCGA GAAGATTCAC AAGACGGGAT TGAAGGTTGT GAGGCTTTGT 
GCGAGATCTC GAGAGCACAG TGAGACTACA GTACCCTACT TGACGCTTCA ACATCAGCTA 
AAAGTGATGG GCGGTGCGGA GCTTCAGAAG CTGATTCAGT TGAAAGATGA AGCCGGGGAG 
CTCGAGTTTA AGGATGATTT GAGATATATG CAATTGAAAA GAGTCAAAGA GCATGAGCTT 
CTCGCCGCCG CTGACGTCAT CTGTTGTACG TGCTCATCGG CGGCTGATGC GAGATTGTCG 
AAGATCCGTA CGAGAACTGT GCTCATTGAT GAGAGCACAC AGGCGACAGA GCCCGAGATT 
TTGGTCTCTA TTATGAGAGG AGTCAGGCAA TTGGTTTTAG TTGGAGATCA CTGTCAACTG 
GGACCGGTTG TTATATGTAA AAAGGCGGCG ATTGCCGGAT TGAGCCAATC TTTGTTCGAG 
AGACTTGTGC TTCTCGGTAT TCGACCGTTC CGGCTTCAGG TGCAATACCG TATGCATCCA 
GTGCTCTCAG AGTTCCCGAG CAACGTGTTT TACGATGGAA GTCTCCAGAA TGGTGTCACT 
GAAAACGATC GCCATATGAC AGGAGTCGAT TGGCATTGGC CTAAACCAAA TAAGCCGGCC 
TTCTTCTGGC ATTGCAGTGG ATCCGAAGAG CTTTCAGCAT CTGGAACCTC ATTTCTCAAT 
AGAACAGAAG CCGCGAATGT GGAGAAACTT GTGTCGAAAT TGATAAAAGC AGGCGTTCAA 
CCACATCAAA TCGGTGTTAT TACTCCGTAT GAGGGACAAC GTAGTTTTAT AGTCAATTAT 
ATGCATACAC AAGGAACTCT CAACTCGAAA CTCTATGAAA ATGTCGAAAT TGCTAGTGTT 
GACGCTTTTC AGGGTCGCGA AAAAGACTAT ATCATCGTAA CGTGTGTTCG ATCGAACGAT 
ATTCTCGGAA TCGGATTTCT CAGTGATCCA CGTCGTCTCA ACGTTGCAAT TACACGTGCC 
AAATACGGTT TGGTTCTAGT CGGAAATGCA AAGGTCTTGG CACGACATGA TCTATGGCAT 
GAGCTGATTA ATCACTATAA ATCGAAGGAA ATGCTCTACG AGGGACCGAT TAACGCGCTG 
AAACCGCTGA ATTTGGCATT GCCAAAGGCG ACGATTCGCA CCAAGAATAA TATAGCGGGC 
AACGCAAATC GATTCGGAAT CAAACGTATG CAATATACAT TCAATGAATA CAAATCCAAT 
GATCCATCAC AACCACGTCT TCCACCAACA TATTCAAACT CACAGAATCT GTTGAGTATG 
AGCAAGTTGG CTCAGACTTT TAACAAGAAT GTGCCCATCC CAGCACATAT GATGGATCCG 
AATGTCTATG CGGCCGCTAG AAATCAGAAA GACCGCCGCC GTGGTGACCA ACGCCGTCCT 
CCTCCACAAG CTGAAGCAGC AATGGATTTA TCGCAAGGAA TGATGTCTCA ACAATCTCAA 
CAATATCCAC CACAAGGAGC TTCTTCTCAA TCACAATATC TTCTCGACGG AGCTTCATCA 
CTATCCGGAT GGTCTCAATC TCAGACCACG ACCACCACCA CGCGTCACCA CCACCACCGT 
CAAAACCGCA ACTCTCAGCA GCAAATGTCT CAGGATATGG ACGATATTCA GCAGAAAATG 
GACGATTTGC TCTTTTCGCA GGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg-2 ( NM_058428.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_490829.1 Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), mRNA.

Price & Availability↑

CloneID	OCa337176
Clone ID Related Accession (Same CDS sequence)	NM_058428.4 , NM_058428.5
Accession Version	NM_058428.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Regulator of nonsense transcripts 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_058428.4.

ATGGACGATT CGGACGACGA ATATTCGAGA AGCCACGGGG AAACCCTCAC ATTTGTGGAT 
CCCGAGGACG ATGGAGTCTC TATTGGGAAC ACGCAGGACT CTCAGTTCGC CTATGAGCAG 
TTTAGTGTGC CTACGCAGAG TTCTCAGGCG ACAGATCTGC TGCCAGGAGG AACCGATGGA 
ACCACCAACG ATTTGCCATT TCACGACGTG GAAGACGACG AGTCGGACAG TGAAAAATCA 
CTGACAGAAG AGCAGCAGCA GCAGAAGCTG CCGGAGCACG CGTGTCGCTA TTGTGGAATC 
TCTGATCCTC TGTGTGTCGC CAAATGCACA GTCTGCAGGA AATGGTTCTG TAATTCCAAT 
GATGGAACGT CGGGCGGGCA TATTGTTCAT CATATGGTTC GGAGTCAGCA TAAGGAAGCC 
TACACGCATA AGGATTCACC GTGTGGTGAC ACTCAATTGG AATGCTATCG TTGTGGTTCG 
AAAAACGTGT TTAATCTCGG CTTCATCCCC GGAAAAAAGG ATCAAGTCGT CGTGATAATC 
TGTAGAACAC CGTGCGCCAG TATTGCATTC CAGAATGATG ATAATTGGTC GCCCGAGGAC 
TGGAAATCTG TAATCGCTGA AAAACAGCTG CTATCGTGGA TTGTTAATGT GCCCAGCGAG 
GAGCAGGTGG CCAGAGCTAG GAAAATCACT GCTACCCAAG CAGTCCGAAT GGAAGAGCTC 
TGGCGTGATC ACCCAGAAGC AACCGTTGAC GATTTAAATA AACCGGGACT CGATCGAGAA 
CCGGATCATG TTCAATTAAG ATATGTGGAT GCTCATCACT ATTCGAAAGT GTTCCGTCCA 
TTAGTTGCAA TTGAAGCAGA ATATGATCGA AGAGTTAAAG AATCAGCATC ACAAGCAGTC 
GGAACTGTTA GATGGGAACA AGGACTTCGT CAATCGGTTC TCGCGTTCTT TCATCTTCCA 
CAATTTGCTG ATGGTGTCAT GAAGCTTGCG AAAGGCGATG AACTTCGATT GAAACATTCT 
CAGACGGTTG ATGGATCGGA ATGGACCAAG ATTGGAAGTG TTTTCAAGAT TCCTGATAAC 
CACGGCGACG AGGTAGGCAT CGAAATCCGC GGAGCTGTCG ACAAATCAGT GATGGAGAGT 
CGGATTATGT TCACCGTCGA CGTCGTCTGG AACGCCACCA CCTTCGAGCG ACAGTACAAG 
GCTCTTGCGG CGCTGCTCAA CGACTCCAAA GCAATATCTC CATATCTCTA CCAGAAGCTT 
CTGGGTCATC CAGCCGAGGA AATGATGCTC AAGTTTGATC TACCGCGTCG GCTATCTGTC 
GCTGGACTCC CCGAGCTTAA TAGCTCCCAG ATGCAGGCAG TGAAGCAGGT GCTCACCCGA 
CCTTTAAGCT TGATTCAAGG ACCTCCGGGA ACTGGAAAAA CCGTAGTTTC GGCGACAATC 
GTCTATCATT TGGTGCAGAA AACCGAGGGA AACGTGCTCG TGTGCTCGCC GAGCAACATC 
GCCGTCGATC ACTTGGCCGA GAAGATTCAC AAGACGGGAT TGAAGGTTGT GAGGCTTTGT 
GCGAGATCTC GAGAGCACAG TGAGACTACA GTACCCTACT TGACGCTTCA ACATCAGCTA 
AAAGTGATGG GCGGTGCGGA GCTTCAGAAG CTGATTCAGT TGAAAGATGA AGCCGGGGAG 
CTCGAGTTTA AGGATGATTT GAGATATATG CAATTGAAAA GAGTCAAAGA GCATGAGCTT 
CTCGCCGCCG CTGACGTCAT CTGTTGTACG TGCTCATCGG CGGCTGATGC GAGATTGTCG 
AAGATCCGTA CGAGAACTGT GCTCATTGAT GAGAGCACAC AGGCGACAGA GCCCGAGATT 
TTGGTCTCTA TTATGAGAGG AGTCAGGCAA TTGGTTTTAG TTGGAGATCA CTGTCAACTG 
GGACCGGTTG TTATATGTAA AAAGGCGGCG ATTGCCGGAT TGAGCCAATC TTTGTTCGAG 
AGACTTGTGC TTCTCGGTAT TCGACCGTTC CGGCTTCAGG TGCAATACCG TATGCATCCA 
GTGCTCTCAG AGTTCCCGAG CAACGTGTTT TACGATGGAA GTCTCCAGAA TGGTGTCACT 
GAAAACGATC GCCATATGAC AGGAGTCGAT TGGCATTGGC CTAAACCAAA TAAGCCGGCC 
TTCTTCTGGC ATTGCAGTGG ATCCGAAGAG CTTTCAGCAT CTGGAACCTC ATTTCTCAAT 
AGAACAGAAG CCGCGAATGT GGAGAAACTT GTGTCGAAAT TGATAAAAGC AGGCGTTCAA 
CCACATCAAA TCGGTGTTAT TACTCCGTAT GAGGGACAAC GTAGTTTTAT AGTCAATTAT 
ATGCATACAC AAGGAACTCT CAACTCGAAA CTCTATGAAA ATGTCGAAAT TGCTAGTGTT 
GACGCTTTTC AGGGTCGCGA AAAAGACTAT ATCATCGTAA CGTGTGTTCG ATCGAACGAT 
ATTCTCGGAA TCGGATTTCT CAGTGATCCA CGTCGTCTCA ACGTTGCAAT TACACGTGCC 
AAATACGGTT TGGTTCTAGT CGGAAATGCA AAGGTCTTGG CACGACATGA TCTATGGCAT 
GAGCTGATTA ATCACTATAA ATCGAAGGAA ATGCTCTACG AGGGACCGAT TAACGCGCTG 
AAACCGCTGA ATTTGGCATT GCCAAAGGCG ACGATTCGCA CCAAGAATAA TATAGCGGGC 
AACGCAAATC GATTCGGAAT CAAACGTATG CAATATACAT TCAATGAATA CAAATCCAAT 
GATCCATCAC AACCACGTCT TCCACCAACA TATTCAAACT CACAGAATCT GTTGAGTATG 
AGCAAGTTGG CTCAGACTTT TAACAAGAAT GTGCCCATCC CAGCACATAT GATGGATCCG 
AATGTCTATG CGGCCGCTAG AAATCAGAAA GACCGCCGCC GTGGTGACCA ACGCCGTCCT 
CCTCCACAAG CTGAAGCAGC AATGGATTTA TCGCAAGGAA TGATGTCTCA ACAATCTCAA 
CAATATCCAC CACAAGGAGC TTCTTCTCAA TCACAATATC TTCTCGACGG AGCTTCATCA 
CTATCCGGAT GGTCTCAATC TCAGACCACG ACCACCACCA CGCGTCACCA CCACCACCGT 
CAAAACCGCA ACTCTCAGCA GCAAATGTCT CAGGATATGG ACGATATTCA GCAGAAAATG 
GACGATTTGC TCTTTTCGCA GGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_490829.1
CDS	3..3212
Translation	MDDSDDEYSRSHGETLTFVDPEDDGVSIGNTQDSQFAYEQFSVPTQSSQATDLLPGGTDGTTNDLPFHDVEDDESDSEKSLTEEQQQQKLPEHACRYCGISDPLCVAKCTVCRKWFCNSNDGTSGGHIVHHMVRSQHKEAYTHKDSPCGDTQLECYRCGSKNVFNLGFIPGKKDQVVVIICRTPCASIAFQNDDNWSPEDWKSVIAEKQLLSWIVNVPSEEQVARARKITATQAVRMEELWRDHPEATVDDLNKPGLDREPDHVQLRYVDAHHYSKVFRPLVAIEAEYDRRVKESASQAVGTVRWEQGLRQSVLAFFHLPQFADGVMKLAKGDELRLKHSQTVDGSEWTKIGSVFKIPDNHGDEVGIEIRGAVDKSVMESRIMFTVDVVWNATTFERQYKALAALLNDSKAISPYLYQKLLGHPAEEMMLKFDLPRRLSVAGLPELNSSQMQAVKQVLTRPLSLIQGPPGTGKTVVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVRQLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRPFRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHCSGSEELSASGTSFLNRTEAANVEKLVSKLIKAGVQPHQIGVITPYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILGIGFLSDPRRLNVAITRAKYGLVLVGNAKVLARHDLWHELINHYKSKEMLYEGPINALKPLNLALPKATIRTKNNIAGNANRFGIKRMQYTFNEYKSNDPSQPRLPPTYSNSQNLLSMSKLAQTFNKNVPIPAHMMDPNVYAAARNQKDRRRGDQRRPPPQAEAAMDLSQGMMSQQSQQYPPQGASSQSQYLLDGASSLSGWSQSQTTTTTTRHHHHRQNRNSQQQMSQDMDDIQQKMDDLLFSQDC

Target ORF information:

RefSeq Version	NM_058428.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Regulator of nonsense transcripts 1 (smg-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058428.5

ORF Insert Sequence:

ATGGACGATT CGGACGACGA ATATTCGAGA AGCCACGGGG AAACCCTCAC ATTTGTGGAT 
CCCGAGGACG ATGGAGTCTC TATTGGGAAC ACGCAGGACT CTCAGTTCGC CTATGAGCAG 
TTTAGTGTGC CTACGCAGAG TTCTCAGGCG ACAGATCTGC TGCCAGGAGG AACCGATGGA 
ACCACCAACG ATTTGCCATT TCACGACGTG GAAGACGACG AGTCGGACAG TGAAAAATCA 
CTGACAGAAG AGCAGCAGCA GCAGAAGCTG CCGGAGCACG CGTGTCGCTA TTGTGGAATC 
TCTGATCCTC TGTGTGTCGC CAAATGCACA GTCTGCAGGA AATGGTTCTG TAATTCCAAT 
GATGGAACGT CGGGCGGGCA TATTGTTCAT CATATGGTTC GGAGTCAGCA TAAGGAAGCC 
TACACGCATA AGGATTCACC GTGTGGTGAC ACTCAATTGG AATGCTATCG TTGTGGTTCG 
AAAAACGTGT TTAATCTCGG CTTCATCCCC GGAAAAAAGG ATCAAGTCGT CGTGATAATC 
TGTAGAACAC CGTGCGCCAG TATTGCATTC CAGAATGATG ATAATTGGTC GCCCGAGGAC 
TGGAAATCTG TAATCGCTGA AAAACAGCTG CTATCGTGGA TTGTTAATGT GCCCAGCGAG 
GAGCAGGTGG CCAGAGCTAG GAAAATCACT GCTACCCAAG CAGTCCGAAT GGAAGAGCTC 
TGGCGTGATC ACCCAGAAGC AACCGTTGAC GATTTAAATA AACCGGGACT CGATCGAGAA 
CCGGATCATG TTCAATTAAG ATATGTGGAT GCTCATCACT ATTCGAAAGT GTTCCGTCCA 
TTAGTTGCAA TTGAAGCAGA ATATGATCGA AGAGTTAAAG AATCAGCATC ACAAGCAGTC 
GGAACTGTTA GATGGGAACA AGGACTTCGT CAATCGGTTC TCGCGTTCTT TCATCTTCCA 
CAATTTGCTG ATGGTGTCAT GAAGCTTGCG AAAGGCGATG AACTTCGATT GAAACATTCT 
CAGACGGTTG ATGGATCGGA ATGGACCAAG ATTGGAAGTG TTTTCAAGAT TCCTGATAAC 
CACGGCGACG AGGTAGGCAT CGAAATCCGC GGAGCTGTCG ACAAATCAGT GATGGAGAGT 
CGGATTATGT TCACCGTCGA CGTCGTCTGG AACGCCACCA CCTTCGAGCG ACAGTACAAG 
GCTCTTGCGG CGCTGCTCAA CGACTCCAAA GCAATATCTC CATATCTCTA CCAGAAGCTT 
CTGGGTCATC CAGCCGAGGA AATGATGCTC AAGTTTGATC TACCGCGTCG GCTATCTGTC 
GCTGGACTCC CCGAGCTTAA TAGCTCCCAG ATGCAGGCAG TGAAGCAGGT GCTCACCCGA 
CCTTTAAGCT TGATTCAAGG ACCTCCGGGA ACTGGAAAAA CCGTAGTTTC GGCGACAATC 
GTCTATCATT TGGTGCAGAA AACCGAGGGA AACGTGCTCG TGTGCTCGCC GAGCAACATC 
GCCGTCGATC ACTTGGCCGA GAAGATTCAC AAGACGGGAT TGAAGGTTGT GAGGCTTTGT 
GCGAGATCTC GAGAGCACAG TGAGACTACA GTACCCTACT TGACGCTTCA ACATCAGCTA 
AAAGTGATGG GCGGTGCGGA GCTTCAGAAG CTGATTCAGT TGAAAGATGA AGCCGGGGAG 
CTCGAGTTTA AGGATGATTT GAGATATATG CAATTGAAAA GAGTCAAAGA GCATGAGCTT 
CTCGCCGCCG CTGACGTCAT CTGTTGTACG TGCTCATCGG CGGCTGATGC GAGATTGTCG 
AAGATCCGTA CGAGAACTGT GCTCATTGAT GAGAGCACAC AGGCGACAGA GCCCGAGATT 
TTGGTCTCTA TTATGAGAGG AGTCAGGCAA TTGGTTTTAG TTGGAGATCA CTGTCAACTG 
GGACCGGTTG TTATATGTAA AAAGGCGGCG ATTGCCGGAT TGAGCCAATC TTTGTTCGAG 
AGACTTGTGC TTCTCGGTAT TCGACCGTTC CGGCTTCAGG TGCAATACCG TATGCATCCA 
GTGCTCTCAG AGTTCCCGAG CAACGTGTTT TACGATGGAA GTCTCCAGAA TGGTGTCACT 
GAAAACGATC GCCATATGAC AGGAGTCGAT TGGCATTGGC CTAAACCAAA TAAGCCGGCC 
TTCTTCTGGC ATTGCAGTGG ATCCGAAGAG CTTTCAGCAT CTGGAACCTC ATTTCTCAAT 
AGAACAGAAG CCGCGAATGT GGAGAAACTT GTGTCGAAAT TGATAAAAGC AGGCGTTCAA 
CCACATCAAA TCGGTGTTAT TACTCCGTAT GAGGGACAAC GTAGTTTTAT AGTCAATTAT 
ATGCATACAC AAGGAACTCT CAACTCGAAA CTCTATGAAA ATGTCGAAAT TGCTAGTGTT 
GACGCTTTTC AGGGTCGCGA AAAAGACTAT ATCATCGTAA CGTGTGTTCG ATCGAACGAT 
ATTCTCGGAA TCGGATTTCT CAGTGATCCA CGTCGTCTCA ACGTTGCAAT TACACGTGCC 
AAATACGGTT TGGTTCTAGT CGGAAATGCA AAGGTCTTGG CACGACATGA TCTATGGCAT 
GAGCTGATTA ATCACTATAA ATCGAAGGAA ATGCTCTACG AGGGACCGAT TAACGCGCTG 
AAACCGCTGA ATTTGGCATT GCCAAAGGCG ACGATTCGCA CCAAGAATAA TATAGCGGGC 
AACGCAAATC GATTCGGAAT CAAACGTATG CAATATACAT TCAATGAATA CAAATCCAAT 
GATCCATCAC AACCACGTCT TCCACCAACA TATTCAAACT CACAGAATCT GTTGAGTATG 
AGCAAGTTGG CTCAGACTTT TAACAAGAAT GTGCCCATCC CAGCACATAT GATGGATCCG 
AATGTCTATG CGGCCGCTAG AAATCAGAAA GACCGCCGCC GTGGTGACCA ACGCCGTCCT 
CCTCCACAAG CTGAAGCAGC AATGGATTTA TCGCAAGGAA TGATGTCTCA ACAATCTCAA 
CAATATCCAC CACAAGGAGC TTCTTCTCAA TCACAATATC TTCTCGACGG AGCTTCATCA 
CTATCCGGAT GGTCTCAATC TCAGACCACG ACCACCACCA CGCGTCACCA CCACCACCGT 
CAAAACCGCA ACTCTCAGCA GCAAATGTCT CAGGATATGG ACGATATTCA GCAGAAAATG 
GACGATTTGC TCTTTTCGCA GGATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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smg-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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