ANO2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following ANO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu23371 XM_006068393.1
Latest version!
Bubalus bubalis anoctamin 2 (ANO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu64014 XM_006068393.2
Latest version!
Bubalus bubalis anoctamin 2 (ANO2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu64015 XM_025283061.1
Latest version!
Bubalus bubalis anoctamin 2 (ANO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu64016 XM_025283062.1
Latest version!
Bubalus bubalis anoctamin 2 (ANO2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu23372 XM_006068394.1
Latest version!
Bubalus bubalis anoctamin 2 (ANO2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu23371
    Clone ID Related Accession (Same CDS sequence) XM_006068393.1
    Accession Version XM_006068393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product anoctamin-2-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785312.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GTCAGAGGGG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GCCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCGTCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TCGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCC GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCAA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACGGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCCGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGGATCT GGTTTGATAT CCTCTCTGGC
    ATCGGCAAAT TCTCTGTTAT CATCAACGCT TTCGTCATCG CCGTCACCTC CGACTTCATC
    CCCCGCCTGG TGTATCAGTA CTCCTACAGT CACAATGGGA CTCTGCACGG CTTTGTCAAC
    CACACCCTCT CCTTTTTCAA CGTCAGCCAG CTGAAGGAGG GAACGCAGCC CGAGAACTCC
    CAGTTTGAGC AGGAGGTTCA GTTCTGCAGG TTTAAGGATT ACCGGGAGCC TCCGTGGGCC
    CCAAACCCGT ACGAGTTTTC CAAGCAGTAC TGGGCTGTTC TGTCTGCACG TCTGGCTTTT
    GTCATAATCT TCCAGAACCT GGTGATGTTC CTGAGTGTCC TCGTGGACTG GATGATCCCT
    GACATCCCCA CGGACATCAG CGAGCAGATC AAGAGGGAGA AGAGCCTGTT TGTGGACTTC
    TTCCTGAAAG AGGAGCACGA GAAACTCAAG CTGGCAGAGG AGCCAGCCCG GAGGAGCCAG
    AGCAGCGGCC ACCGCAGCAG AAGGGGCCCA GCAGCCAGCC CGGCGCCCTC TGGCCGGAAC
    CAGCCGGGCA GCAGTGCGTC CTCGGGGTCC CAGCACACCA GCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068455.1
    CDS67..3054
    Translation

    Target ORF information:

    RefSeq Version XM_006068393.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis anoctamin 2 (ANO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GTCAGAGGGG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GCCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCGTCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TCGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCC GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCAA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACGGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCCGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGGATCT GGTTTGATAT CCTCTCTGGC
    ATCGGCAAAT TCTCTGTTAT CATCAACGCT TTCGTCATCG CCGTCACCTC CGACTTCATC
    CCCCGCCTGG TGTATCAGTA CTCCTACAGT CACAATGGGA CTCTGCACGG CTTTGTCAAC
    CACACCCTCT CCTTTTTCAA CGTCAGCCAG CTGAAGGAGG GAACGCAGCC CGAGAACTCC
    CAGTTTGAGC AGGAGGTTCA GTTCTGCAGG TTTAAGGATT ACCGGGAGCC TCCGTGGGCC
    CCAAACCCGT ACGAGTTTTC CAAGCAGTAC TGGGCTGTTC TGTCTGCACG TCTGGCTTTT
    GTCATAATCT TCCAGAACCT GGTGATGTTC CTGAGTGTCC TCGTGGACTG GATGATCCCT
    GACATCCCCA CGGACATCAG CGAGCAGATC AAGAGGGAGA AGAGCCTGTT TGTGGACTTC
    TTCCTGAAAG AGGAGCACGA GAAACTCAAG CTGGCAGAGG AGCCAGCCCG GAGGAGCCAG
    AGCAGCGGCC ACCGCAGCAG AAGGGGCCCA GCAGCCAGCC CGGCGCCCTC TGGCCGGAAC
    CAGCCGGGCA GCAGTGCGTC CTCGGGGTCC CAGCACACCA GCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu64014
    Clone ID Related Accession (Same CDS sequence) XM_006068393.2
    Accession Version XM_006068393.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product anoctamin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006068393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GCCAGAGGAG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GGCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCAGCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TGGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCA GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCGA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACAGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCTGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGGATCT GGTTTGATAT CCTCTCTGGC
    ATCGGCAAAT TCTCTGTTAT CATCAACGCT TTCGTCATCG CCGTCACCTC CGACTTCATC
    CCCCGCCTGG TGTATCAGTA CTCCTACAGT CACAATGGGA CTCTGCACGG CTTTGTCAAC
    CACACCCTCT CCTTTTTCAA CGTCAGCCAG CTGAAGGAGG GAACGCAGCC CGAGAACTCC
    CAGTTTGAGC AGGAGGTTCA GTTCTGCAGG TTTAAGGATT ACCGGGAGCC TCCGTGGGCC
    CCAAACCCGT ACGAGTTTTC CAAGCAGTAC TGGGCTGTTC TGTCTGCACG TCTGGCTTTT
    GTCATAATCT TCCAGAACCT GGTGATGTTC CTGAGTGTCC TCGTGGACTG GATGATCCCT
    GACATCCCCA CGGACATCAG CGAGCAGATC AAGAGGGAGA AGAGCCTGTT TGTGGACTTC
    TTCCTGAAAG AGGAGCACGA GAAACTCAAG CTGGCAGAGG AGCCAGCCCG GAGGAGCCAG
    AGCAGCGGCC ACCGCAGCAG AAGGGGCCCA GCAGCCAGCC CGGCGCCCTC TGGCCGGAAC
    CAGCCGGGCA GCAGTGCGTC CTCGGGGTCC CAGCACACCA GCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068455.2
    CDS72..3059
    Translation

    Target ORF information:

    RefSeq Version XM_006068393.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis anoctamin 2 (ANO2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068393.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GCCAGAGGAG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GGCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCAGCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TGGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCA GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCGA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACAGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCTGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGGATCT GGTTTGATAT CCTCTCTGGC
    ATCGGCAAAT TCTCTGTTAT CATCAACGCT TTCGTCATCG CCGTCACCTC CGACTTCATC
    CCCCGCCTGG TGTATCAGTA CTCCTACAGT CACAATGGGA CTCTGCACGG CTTTGTCAAC
    CACACCCTCT CCTTTTTCAA CGTCAGCCAG CTGAAGGAGG GAACGCAGCC CGAGAACTCC
    CAGTTTGAGC AGGAGGTTCA GTTCTGCAGG TTTAAGGATT ACCGGGAGCC TCCGTGGGCC
    CCAAACCCGT ACGAGTTTTC CAAGCAGTAC TGGGCTGTTC TGTCTGCACG TCTGGCTTTT
    GTCATAATCT TCCAGAACCT GGTGATGTTC CTGAGTGTCC TCGTGGACTG GATGATCCCT
    GACATCCCCA CGGACATCAG CGAGCAGATC AAGAGGGAGA AGAGCCTGTT TGTGGACTTC
    TTCCTGAAAG AGGAGCACGA GAAACTCAAG CTGGCAGAGG AGCCAGCCCG GAGGAGCCAG
    AGCAGCGGCC ACCGCAGCAG AAGGGGCCCA GCAGCCAGCC CGGCGCCCTC TGGCCGGAAC
    CAGCCGGGCA GCAGTGCGTC CTCGGGGTCC CAGCACACCA GCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu64015
    Clone ID Related Accession (Same CDS sequence) XM_025283061.1
    Accession Version XM_025283061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2733bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product anoctamin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCACTTCC ATGACAACCA GAGGAAGGTG GACTACGTGC TTGCCTACCA CTACCGGAAA 
    CGCGGGGCGC ACCCCGGCCA CAGCTCCCCG GGCCACAATC TGGCTATTGT CTCCAATGGC
    GAGACGGGCA AGGACCCCCA GGCGGGGGCT CCCGGTGACA TCGAGCTGGG GCCGCTGGAT
    GCCCTGGAGG AGGAGAGGAA GGAGCAGCGA GAGGAGTTTG AGCACAACCT GATGGAGGCT
    GGGCTGGAGC TGGAGAAGGA CTTGGAGAGC AAAAGCCAGG GCTCCGTCTT CGTCCGGATC
    CACGCCCCGT GGCAGGTGCT GGCCAGAGAG GCGGAATTCT TGAAGATCAA AGTTCCCACC
    AAGAAAATGT ACGAGATCAA GTCACAAGGC AGCATTGCAA AGACGTTCAA CAAGATTCTG
    CACAAGCTGA GTGCGCCCCT GAAGCCTCGA GTGCCCGAGC ACAGTAACAG CAGGATGAAG
    AACCTCTCGT ACCCGTTCTC CAGGGAGAAA ATGTACCTGT ACAACATCCA GGACAAGGAC
    ACCTTCTTTG ATAATGCCAC CCGTAGTCGC ATTGTGCATG AGATCCTGAA GCGCACAACC
    TGCTCTCGAG CCAACAACAC GATGGGTATT AACTCTCTGA TAGCAAACAA TATCTATGAG
    GCTGCCTACC CATTGCACGA TGGTGAATAT GACAGTCCAG GGGATGATAT GAATGACAGA
    AAGCTGCTGT ATCAAGAATG GGCACGCTAC GGGGTGTTTT ATAAGTTTCA ACCCATTGAC
    CTCATCAGAA AGTATTTTGG AGAAAAGATC GGACTATATT TTGCCTGGCT GGGATTATAT
    ACATCCTTCC TCATCCCGTC ATCTGTCATC GGGATCATCG TGTTTCTTTA CGGATGTGCA
    ACCATCGAAG AAGATATTCC CAGCAAGGAG ATGTGTGACC AGCAGAACGC CTTCACCATG
    TGTCCCCTGT GCGACACGTC CTGTGACTAC TGGAACCTCA GCTCGGCCTG TGGGACAGCG
    CGGGCCAGCC ACCTGTTTGA CAACCCTGCC ACCGTCTTCT TCTCCATCTT CATGGCTCTG
    TGGGCTACCA TGTTTCTTGA AAACTGGAAG CGATTACAGA TGCGACTGGG CTACTTCTGG
    GACCTGACTG GCATTGAAGA GGAAGAAGAG CATTCCCGGC CTGAGTATGA AACGAAAGTT
    CGTGAGAAAA TGCTGAAGGA GAGCAGCAAG TCTGTGGTCC AGAAACTGGG AACCAGTGGG
    ACTGAGGCTG AGGATGAGGA CGATGAAGAT AAGCTGACCT GGAAAGATCG TTTCCCAGGT
    TACCTGATGA ACTTCGCCTC CATCTTGTTC ATGATCGCCT TGACGTTCTC CATCGTCTTT
    GGGGTCATCG TCTATCGCAT CACTACTGCG GCTGCTCTGT CTCTCAACAA GGCCACACGC
    TCCAACGTCC GGGTGACGGT GACGGCCACA GCGGTCATCA TCAACCTGGT GGTCATTCTC
    ATTCTGGACG AGATCTACGG CGCCGTGGCC AAGTGGCTCA CCAAAATCGA GGTTCCAAAA
    ACAGAGCAGA CATTTGAAGA GCGCCTGATC CTCAAGGCTT TCTTACTGAA GTTTGTCAAT
    GCCTACTCAC CCATCTTCTA TGTGGCTTTT TTCAAGGGAA GGTTTGTCGG TAGACCCGGA
    AGCTATGTTT ATGTGTTTGA TGGTTACCGC ATGGAAGAGT GCGCCCCTGG GGGCTGCCTC
    ATGGAGCTGT GCATCCAACT CAGCATCATC ATGTTGGGGA AGCAGTTGAT CCAGAACAAC
    ATCTTTGAGA TCGGAGTCCC GAAGCTAAAG AAGCTATTTC GGAAGCTGAA AGATGAGACA
    GAGCCTGAAG AAGCTGACTC TGCCCATTCA AAGCATCCAG AGCAGTGGGA CCTAGACTAC
    AGCTTGGAAC CGTACACTGG ACTGACGCCG GAGTATATGG AAATGATCAT CCAGTTTGGC
    TTTGTCACCC TCTTCGTGGC CTCCTTTCCT CTGGCACCTG TGTTCGCCCT CCTCAACAAC
    GTCATTGAAG TGCGCCTGGA TGCGAAGAAG TTCGTTACAG AGCTGAGACG GCCAGATGCT
    GTGAGGACCA AGGATATCGG GATCTGGTTT GATATCCTCT CTGGCATCGG CAAATTCTCT
    GTTATCATCA ACGCTTTCGT CATCGCCGTC ACCTCCGACT TCATCCCCCG CCTGGTGTAT
    CAGTACTCCT ACAGTCACAA TGGGACTCTG CACGGCTTTG TCAACCACAC CCTCTCCTTT
    TTCAACGTCA GCCAGCTGAA GGAGGGAACG CAGCCCGAGA ACTCCCAGTT TGAGCAGGAG
    GTTCAGTTCT GCAGGTTTAA GGATTACCGG GAGCCTCCGT GGGCCCCAAA CCCGTACGAG
    TTTTCCAAGC AGTACTGGGC TGTTCTGTCT GCACGTCTGG CTTTTGTCAT AATCTTCCAG
    AACCTGGTGA TGTTCCTGAG TGTCCTCGTG GACTGGATGA TCCCTGACAT CCCCACGGAC
    ATCAGCGAGC AGATCAAGAG GGAGAAGAGC CTGTTTGTGG ACTTCTTCCT GAAAGAGGAG
    CACGAGAAAC TCAAGCTGGC AGAGGAGCCA GCCCGGAGGA GCCAGAGCAG CGGCCACCGC
    AGCAGAAGGG GCCCAGCAGC CAGCCCGGCG CCCTCTGGCC GGAACCAGCC GGGCAGCAGT
    GCGTCCTCGG GGTCCCAGCA CACCAGCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025138846.1
    CDS358..3090
    Translation

    Target ORF information:

    RefSeq Version XM_025283061.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis anoctamin 2 (ANO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025283061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCACTTCC ATGACAACCA GAGGAAGGTG GACTACGTGC TTGCCTACCA CTACCGGAAA 
    CGCGGGGCGC ACCCCGGCCA CAGCTCCCCG GGCCACAATC TGGCTATTGT CTCCAATGGC
    GAGACGGGCA AGGACCCCCA GGCGGGGGCT CCCGGTGACA TCGAGCTGGG GCCGCTGGAT
    GCCCTGGAGG AGGAGAGGAA GGAGCAGCGA GAGGAGTTTG AGCACAACCT GATGGAGGCT
    GGGCTGGAGC TGGAGAAGGA CTTGGAGAGC AAAAGCCAGG GCTCCGTCTT CGTCCGGATC
    CACGCCCCGT GGCAGGTGCT GGCCAGAGAG GCGGAATTCT TGAAGATCAA AGTTCCCACC
    AAGAAAATGT ACGAGATCAA GTCACAAGGC AGCATTGCAA AGACGTTCAA CAAGATTCTG
    CACAAGCTGA GTGCGCCCCT GAAGCCTCGA GTGCCCGAGC ACAGTAACAG CAGGATGAAG
    AACCTCTCGT ACCCGTTCTC CAGGGAGAAA ATGTACCTGT ACAACATCCA GGACAAGGAC
    ACCTTCTTTG ATAATGCCAC CCGTAGTCGC ATTGTGCATG AGATCCTGAA GCGCACAACC
    TGCTCTCGAG CCAACAACAC GATGGGTATT AACTCTCTGA TAGCAAACAA TATCTATGAG
    GCTGCCTACC CATTGCACGA TGGTGAATAT GACAGTCCAG GGGATGATAT GAATGACAGA
    AAGCTGCTGT ATCAAGAATG GGCACGCTAC GGGGTGTTTT ATAAGTTTCA ACCCATTGAC
    CTCATCAGAA AGTATTTTGG AGAAAAGATC GGACTATATT TTGCCTGGCT GGGATTATAT
    ACATCCTTCC TCATCCCGTC ATCTGTCATC GGGATCATCG TGTTTCTTTA CGGATGTGCA
    ACCATCGAAG AAGATATTCC CAGCAAGGAG ATGTGTGACC AGCAGAACGC CTTCACCATG
    TGTCCCCTGT GCGACACGTC CTGTGACTAC TGGAACCTCA GCTCGGCCTG TGGGACAGCG
    CGGGCCAGCC ACCTGTTTGA CAACCCTGCC ACCGTCTTCT TCTCCATCTT CATGGCTCTG
    TGGGCTACCA TGTTTCTTGA AAACTGGAAG CGATTACAGA TGCGACTGGG CTACTTCTGG
    GACCTGACTG GCATTGAAGA GGAAGAAGAG CATTCCCGGC CTGAGTATGA AACGAAAGTT
    CGTGAGAAAA TGCTGAAGGA GAGCAGCAAG TCTGTGGTCC AGAAACTGGG AACCAGTGGG
    ACTGAGGCTG AGGATGAGGA CGATGAAGAT AAGCTGACCT GGAAAGATCG TTTCCCAGGT
    TACCTGATGA ACTTCGCCTC CATCTTGTTC ATGATCGCCT TGACGTTCTC CATCGTCTTT
    GGGGTCATCG TCTATCGCAT CACTACTGCG GCTGCTCTGT CTCTCAACAA GGCCACACGC
    TCCAACGTCC GGGTGACGGT GACGGCCACA GCGGTCATCA TCAACCTGGT GGTCATTCTC
    ATTCTGGACG AGATCTACGG CGCCGTGGCC AAGTGGCTCA CCAAAATCGA GGTTCCAAAA
    ACAGAGCAGA CATTTGAAGA GCGCCTGATC CTCAAGGCTT TCTTACTGAA GTTTGTCAAT
    GCCTACTCAC CCATCTTCTA TGTGGCTTTT TTCAAGGGAA GGTTTGTCGG TAGACCCGGA
    AGCTATGTTT ATGTGTTTGA TGGTTACCGC ATGGAAGAGT GCGCCCCTGG GGGCTGCCTC
    ATGGAGCTGT GCATCCAACT CAGCATCATC ATGTTGGGGA AGCAGTTGAT CCAGAACAAC
    ATCTTTGAGA TCGGAGTCCC GAAGCTAAAG AAGCTATTTC GGAAGCTGAA AGATGAGACA
    GAGCCTGAAG AAGCTGACTC TGCCCATTCA AAGCATCCAG AGCAGTGGGA CCTAGACTAC
    AGCTTGGAAC CGTACACTGG ACTGACGCCG GAGTATATGG AAATGATCAT CCAGTTTGGC
    TTTGTCACCC TCTTCGTGGC CTCCTTTCCT CTGGCACCTG TGTTCGCCCT CCTCAACAAC
    GTCATTGAAG TGCGCCTGGA TGCGAAGAAG TTCGTTACAG AGCTGAGACG GCCAGATGCT
    GTGAGGACCA AGGATATCGG GATCTGGTTT GATATCCTCT CTGGCATCGG CAAATTCTCT
    GTTATCATCA ACGCTTTCGT CATCGCCGTC ACCTCCGACT TCATCCCCCG CCTGGTGTAT
    CAGTACTCCT ACAGTCACAA TGGGACTCTG CACGGCTTTG TCAACCACAC CCTCTCCTTT
    TTCAACGTCA GCCAGCTGAA GGAGGGAACG CAGCCCGAGA ACTCCCAGTT TGAGCAGGAG
    GTTCAGTTCT GCAGGTTTAA GGATTACCGG GAGCCTCCGT GGGCCCCAAA CCCGTACGAG
    TTTTCCAAGC AGTACTGGGC TGTTCTGTCT GCACGTCTGG CTTTTGTCAT AATCTTCCAG
    AACCTGGTGA TGTTCCTGAG TGTCCTCGTG GACTGGATGA TCCCTGACAT CCCCACGGAC
    ATCAGCGAGC AGATCAAGAG GGAGAAGAGC CTGTTTGTGG ACTTCTTCCT GAAAGAGGAG
    CACGAGAAAC TCAAGCTGGC AGAGGAGCCA GCCCGGAGGA GCCAGAGCAG CGGCCACCGC
    AGCAGAAGGG GCCCAGCAGC CAGCCCGGCG CCCTCTGGCC GGAACCAGCC GGGCAGCAGT
    GCGTCCTCGG GGTCCCAGCA CACCAGCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu64016
    Clone ID Related Accession (Same CDS sequence) XM_025283062.1
    Accession Version XM_025283062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product anoctamin-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GCCAGAGGAG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GGCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCAGCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TGGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCA GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCGA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACAGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCTGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGCTTTC GTCATCGCCG TCACCTCCGA
    CTTCATCCCC CGCCTGGTGT ATCAGTACTC CTACAGTCAC AATGGGACTC TGCACGGCTT
    TGTCAACCAC ACCCTCTCCT TTTTCAACGT CAGCCAGCTG AAGGAGGGAA CGCAGCCCGA
    GAACTCCCAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025138847.1
    CDS72..2606
    Translation

    Target ORF information:

    RefSeq Version XM_025283062.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis anoctamin 2 (ANO2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025283062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GCCAGAGGAG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GGCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCAGCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TGGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCA GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AATGTACGAG ATCAAGTCAC AAGGCAGCAT TGCAAAGACG
    TTCAACAAGA TTCTGCACAA GCTGAGTGCG CCCCTGAAGC CTCGAGTGCC CGAGCACAGT
    AACAGCAGGA TGAAGAACCT CTCGTACCCG TTCTCCAGGG AGAAAATGTA CCTGTACAAC
    ATCCAGGACA AGGACACCTT CTTTGATAAT GCCACCCGTA GTCGCATTGT GCATGAGATC
    CTGAAGCGCA CAACCTGCTC TCGAGCCAAC AACACGATGG GTATTAACTC TCTGATAGCA
    AACAATATCT ATGAGGCTGC CTACCCATTG CACGATGGTG AATATGACAG TCCAGGGGAT
    GATATGAATG ACAGAAAGCT GCTGTATCAA GAATGGGCAC GCTACGGGGT GTTTTATAAG
    TTTCAACCCA TTGACCTCAT CAGAAAGTAT TTTGGAGAAA AGATCGGACT ATATTTTGCC
    TGGCTGGGAT TATATACATC CTTCCTCATC CCGTCATCTG TCATCGGGAT CATCGTGTTT
    CTTTACGGAT GTGCAACCAT CGAAGAAGAT ATTCCCAGCA AGGAGATGTG TGACCAGCAG
    AACGCCTTCA CCATGTGTCC CCTGTGCGAC ACGTCCTGTG ACTACTGGAA CCTCAGCTCG
    GCCTGTGGGA CAGCGCGGGC CAGCCACCTG TTTGACAACC CTGCCACCGT CTTCTTCTCC
    ATCTTCATGG CTCTGTGGGC TACCATGTTT CTTGAAAACT GGAAGCGATT ACAGATGCGA
    CTGGGCTACT TCTGGGACCT GACTGGCATT GAAGAGGAAG AAGAGCATTC CCGGCCTGAG
    TATGAAACGA AAGTTCGTGA GAAAATGCTG AAGGAGAGCA GCAAGTCTGT GGTCCAGAAA
    CTGGGAACCA GTGGGACTGA GGCTGAGGAT GAGGACGATG AAGATAAGCT GACCTGGAAA
    GATCGTTTCC CAGGTTACCT GATGAACTTC GCCTCCATCT TGTTCATGAT CGCCTTGACG
    TTCTCCATCG TCTTTGGGGT CATCGTCTAT CGCATCACTA CTGCGGCTGC TCTGTCTCTC
    AACAAGGCCA CACGCTCCAA CGTCCGGGTG ACGGTGACGG CCACAGCGGT CATCATCAAC
    CTGGTGGTCA TTCTCATTCT GGACGAGATC TACGGCGCCG TGGCCAAGTG GCTCACCAAA
    ATCGAGGTTC CAAAAACAGA GCAGACATTT GAAGAGCGCC TGATCCTCAA GGCTTTCTTA
    CTGAAGTTTG TCAATGCCTA CTCACCCATC TTCTATGTGG CTTTTTTCAA GGGAAGGTTT
    GTCGGTAGAC CCGGAAGCTA TGTTTATGTG TTTGATGGTT ACCGCATGGA AGAGTGCGCC
    CCTGGGGGCT GCCTCATGGA GCTGTGCATC CAACTCAGCA TCATCATGTT GGGGAAGCAG
    TTGATCCAGA ACAACATCTT TGAGATCGGA GTCCCGAAGC TAAAGAAGCT ATTTCGGAAG
    CTGAAAGATG AGACAGAGCC TGAAGAAGCT GACTCTGCCC ATTCAAAGCA TCCAGAGCAG
    TGGGACCTAG ACTACAGCTT GGAACCGTAC ACTGGACTGA CGCCGGAGTA TATGGAAATG
    ATCATCCAGT TTGGCTTTGT CACCCTCTTC GTGGCCTCCT TTCCTCTGGC ACCTGTGTTC
    GCCCTCCTCA ACAACGTCAT TGAAGTGCGC CTGGATGCGA AGAAGTTCGT TACAGAGCTG
    AGACGGCCAG ATGCTGTGAG GACCAAGGAT ATCGGCTTTC GTCATCGCCG TCACCTCCGA
    CTTCATCCCC CGCCTGGTGT ATCAGTACTC CTACAGTCAC AATGGGACTC TGCACGGCTT
    TGTCAACCAC ACCCTCTCCT TTTTCAACGT CAGCCAGCTG AAGGAGGGAA CGCAGCCCGA
    GAACTCCCAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu23372
    Clone ID Related Accession (Same CDS sequence) XM_006068394.1
    Accession Version XM_006068394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product anoctamin-2-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785312.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GTCAGAGGGG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GCCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCGTCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TCGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCC GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AGAGATGTAC GAGATCAAGT CACAAGGCAG CATTGCAAAG
    ACGTTCAACA AGATTCTGCA CAAGCTGAGT GCGCCCCTGA AGCCTCGAGT GCCCGAGCAC
    AGTAACAGCA GGATGAAGAA CCTCTCGTAC CCGTTCTCCA GGGAGAAAAT GTACCTGTAC
    AACATCCAGG ACAAGGACAC CTTCTTTGAT AATGCCACCC GTAGTCGCAT TGTGCATGAG
    ATCCTGAAGC GCACAACCTG CTCTCGAGCC AACAACACGA TGGGTATTAA CTCTCTGATA
    GCAAACAATA TCTATGAGGC TGCCTACCCA TTGCACGATG GTGAATATGA CAGTCCAGGG
    GATGATATGA ATGACAGAAA GCTGCTGTAT CAAGAATGGG CACGCTACGG GGTGTTTTAT
    AAGTTTCAAC CCATTGACCT CATCAGAAAG TATTTTGGAG AAAAGATCGG ACTATATTTT
    GCCTGGCTGG GATTATATAC ATCCTTCCTC ATCCCGTCAT CTGTCATCGG GATCATCGTG
    TTTCTTTACG GATGTGCAAC CATCGAAGAA GATATTCCCA GCAAGGAGAT GTGTGACCAG
    CAGAACGCCT TCACCATGTG TCCCCTGTGC GACACGTCCT GTGACTACTG GAACCTCAGC
    TCGGCCTGTG GGACAGCGCG GGCCAGCCAC CTGTTTGACA ACCCTGCCAC CGTCTTCTTC
    TCCATCTTCA TGGCTCTGTG GGCTACCATG TTTCTTGAAA ACTGGAAGCG ATTACAGATG
    CAACTGGGCT ACTTCTGGGA CCTGACTGGC ATTGAAGAGG AAGAAGAACG TGCTCAGGAG
    CATTCCCGGC CTGAGTATGA AACGAAAGTT CGTGAGAAAA TGCTGAAGGA GAGCAGCAAG
    TCTGTGGTCC AGAAACTGGG AACCAGTGGG ACTGAGGCTG AGGATGAGGA CGATGAAGAT
    AAGCTGACCT GGAAAGATCG TTTCCCAGGT TACCTGATGA ACTTCGCCTC CATCTTGTTC
    ATGATCGCCT TGACGTTCTC CATCGTCTTT GGGGTCATCG TCTATCGCAT CACTACTGCG
    GCTGCTCTGT CTCTCAACAA GGCCACACGC TCCAACGTCC GGGTGACGGT GACGGCCACG
    GCGGTCATCA TCAACCTGGT GGTCATTCTC ATTCTGGACG AGATCTACGG CGCCGTGGCC
    AAGTGGCTCA CCAAAATCGA GGTTCCAAAA ACAGAGCAGA CATTTGAAGA GCGCCTGATC
    CTCAAGGCTT TCTTACTGAA GTTTGTCAAT GCCTACTCAC CCATCTTCTA TGTGGCTTTT
    TTCAAGGGAA GGTTTGTCGG TAGACCCGGA AGCTATGTTT ATGTGTTTGA TGGTTACCGC
    ATGGAAGAGT GCGCCCCCGG GGGCTGCCTC ATGGAGCTGT GCATCCAACT CAGCATCATC
    ATGTTGGGGA AGCAGTTGAT CCAGAACAAC ATCTTTGAGA TCGGAGTCCC GAAGCTAAAG
    AAGCTATTTC GGAAGCTGAA AGATGAGACA GAGCCTGAAG AAGCTGACTC TGCCCATTCA
    AAGCATCCAG AGCAGTGGGA CCTAGACTAC AGCTTGGAAC CGTACACTGG ACTGACGCCG
    GAGTATATGG AAATGATCAT CCAGTTTGGC TTTGTCACCC TCTTCGTGGC CTCCTTTCCT
    CTGGCACCTG TGTTCGCCCT CCTCAACAAC GTCATTGAAG TGCGCCTGGA TGCGAAGAAG
    TTCGTTACAG AGCTGAGACG GCCAGATGCT GTGAGGACCA AGGATATCGG GATCTGGTTT
    GATATCCTCT CTGGCATCGG CAAATTCTCT GTTATCATCA ACGCTTTCGT CATCGCCGTC
    ACCTCCGACT TCATCCCCCG CCTGGTGTAT CAGTACTCCT ACAGTCACAA TGGGACTCTG
    CACGGCTTTG TCAACCACAC CCTCTCCTTT TTCAACGTCA GCCAGCTGAA GGAGGGAACG
    CAGCCCGAGA ACTCCCAGTT TGAGCAGGAG GTTCAGTTCT GCAGGTTTAA GGATTACCGG
    GAGCCTCCGT GGGCCCCAAA CCCGTACGAG TTTTCCAAGC AGTACTGGGC TGTTCTGTCT
    GCACGTCTGG CTTTTGTCAT AATCTTCCAG AACCTGGTGA TGTTCCTGAG TGTCCTCGTG
    GACTGGATGA TCCCTGACAT CCCCACGGAC ATCAGCGAGC AGATCAAGAG GGAGAAGAGC
    CTGTTTGTGG ACTTCTTCCT GAAAGAGGAG CACGAGAAAC TCAAGCTGGC AGAGGAGCCA
    GCCCGGAGGA GCCAGAGCAG CGGCCACCGC AGCAGAAGGG GCCCAGCAGC CAGCCCGGCG
    CCCTCTGGCC GGAACCAGCC GGGCAGCAGT GCGTCCTCGG GGTCCCAGCA CACCAGCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068456.1
    CDS67..3069
    Translation

    Target ORF information:

    RefSeq Version XM_006068394.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis anoctamin 2 (ANO2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATTTTG ACATCCCCCT GCTCCCTGGC TCCCCACGCC GGCTGAGCCC GCGGGCAGTG 
    GTCAGAGGGG GTCAGGGCCC CAAACACGGA CAGCAATGCC TCCAGGTGCC GGGTCCCCCA
    GCCCAGGGTC TGCAGGGCTG TGTCCCTCAA GGTTCCGTCC AGGCGTCCGG TGGAGGGAGC
    CCCCCCAACA GCTCTGTCAT CAACAACTAC TTGGAGGCCA GCGAGCCGGT GTCTTCGGAG
    GCCCGCGTGA GCCGCATGCA CTTCCATGAC AACCAGAGGA AGGTGGACTA CGTGCTTGCC
    TACCACTACC GGAAACGCGG GGCGCACCCC GGCCACAGCT CCCCGGGCCA CAATCTGGCT
    ATTGTCTCCA ATGGCGAGAC GGGCAAGGAC CCCCAGGCGG GGGCTCCCGG TGACATCGAG
    CTGGGGCCGC TCGATGCCCT GGAGGAGGAG AGGAAGGAGC AGCGAGAGGA GTTTGAGCAC
    AACCTGATGG AGGCTGGGCT GGAGCTGGAG AAGGACTTGG AGAGCAAAAG CCAGGGCTCC
    GTCTTCGTCC GGATCCACGC CCCGTGGCAG GTGCTGGCCC GAGAGGCGGA ATTCTTGAAG
    ATCAAAGTTC CCACCAAGAA AGAGATGTAC GAGATCAAGT CACAAGGCAG CATTGCAAAG
    ACGTTCAACA AGATTCTGCA CAAGCTGAGT GCGCCCCTGA AGCCTCGAGT GCCCGAGCAC
    AGTAACAGCA GGATGAAGAA CCTCTCGTAC CCGTTCTCCA GGGAGAAAAT GTACCTGTAC
    AACATCCAGG ACAAGGACAC CTTCTTTGAT AATGCCACCC GTAGTCGCAT TGTGCATGAG
    ATCCTGAAGC GCACAACCTG CTCTCGAGCC AACAACACGA TGGGTATTAA CTCTCTGATA
    GCAAACAATA TCTATGAGGC TGCCTACCCA TTGCACGATG GTGAATATGA CAGTCCAGGG
    GATGATATGA ATGACAGAAA GCTGCTGTAT CAAGAATGGG CACGCTACGG GGTGTTTTAT
    AAGTTTCAAC CCATTGACCT CATCAGAAAG TATTTTGGAG AAAAGATCGG ACTATATTTT
    GCCTGGCTGG GATTATATAC ATCCTTCCTC ATCCCGTCAT CTGTCATCGG GATCATCGTG
    TTTCTTTACG GATGTGCAAC CATCGAAGAA GATATTCCCA GCAAGGAGAT GTGTGACCAG
    CAGAACGCCT TCACCATGTG TCCCCTGTGC GACACGTCCT GTGACTACTG GAACCTCAGC
    TCGGCCTGTG GGACAGCGCG GGCCAGCCAC CTGTTTGACA ACCCTGCCAC CGTCTTCTTC
    TCCATCTTCA TGGCTCTGTG GGCTACCATG TTTCTTGAAA ACTGGAAGCG ATTACAGATG
    CAACTGGGCT ACTTCTGGGA CCTGACTGGC ATTGAAGAGG AAGAAGAACG TGCTCAGGAG
    CATTCCCGGC CTGAGTATGA AACGAAAGTT CGTGAGAAAA TGCTGAAGGA GAGCAGCAAG
    TCTGTGGTCC AGAAACTGGG AACCAGTGGG ACTGAGGCTG AGGATGAGGA CGATGAAGAT
    AAGCTGACCT GGAAAGATCG TTTCCCAGGT TACCTGATGA ACTTCGCCTC CATCTTGTTC
    ATGATCGCCT TGACGTTCTC CATCGTCTTT GGGGTCATCG TCTATCGCAT CACTACTGCG
    GCTGCTCTGT CTCTCAACAA GGCCACACGC TCCAACGTCC GGGTGACGGT GACGGCCACG
    GCGGTCATCA TCAACCTGGT GGTCATTCTC ATTCTGGACG AGATCTACGG CGCCGTGGCC
    AAGTGGCTCA CCAAAATCGA GGTTCCAAAA ACAGAGCAGA CATTTGAAGA GCGCCTGATC
    CTCAAGGCTT TCTTACTGAA GTTTGTCAAT GCCTACTCAC CCATCTTCTA TGTGGCTTTT
    TTCAAGGGAA GGTTTGTCGG TAGACCCGGA AGCTATGTTT ATGTGTTTGA TGGTTACCGC
    ATGGAAGAGT GCGCCCCCGG GGGCTGCCTC ATGGAGCTGT GCATCCAACT CAGCATCATC
    ATGTTGGGGA AGCAGTTGAT CCAGAACAAC ATCTTTGAGA TCGGAGTCCC GAAGCTAAAG
    AAGCTATTTC GGAAGCTGAA AGATGAGACA GAGCCTGAAG AAGCTGACTC TGCCCATTCA
    AAGCATCCAG AGCAGTGGGA CCTAGACTAC AGCTTGGAAC CGTACACTGG ACTGACGCCG
    GAGTATATGG AAATGATCAT CCAGTTTGGC TTTGTCACCC TCTTCGTGGC CTCCTTTCCT
    CTGGCACCTG TGTTCGCCCT CCTCAACAAC GTCATTGAAG TGCGCCTGGA TGCGAAGAAG
    TTCGTTACAG AGCTGAGACG GCCAGATGCT GTGAGGACCA AGGATATCGG GATCTGGTTT
    GATATCCTCT CTGGCATCGG CAAATTCTCT GTTATCATCA ACGCTTTCGT CATCGCCGTC
    ACCTCCGACT TCATCCCCCG CCTGGTGTAT CAGTACTCCT ACAGTCACAA TGGGACTCTG
    CACGGCTTTG TCAACCACAC CCTCTCCTTT TTCAACGTCA GCCAGCTGAA GGAGGGAACG
    CAGCCCGAGA ACTCCCAGTT TGAGCAGGAG GTTCAGTTCT GCAGGTTTAA GGATTACCGG
    GAGCCTCCGT GGGCCCCAAA CCCGTACGAG TTTTCCAAGC AGTACTGGGC TGTTCTGTCT
    GCACGTCTGG CTTTTGTCAT AATCTTCCAG AACCTGGTGA TGTTCCTGAG TGTCCTCGTG
    GACTGGATGA TCCCTGACAT CCCCACGGAC ATCAGCGAGC AGATCAAGAG GGAGAAGAGC
    CTGTTTGTGG ACTTCTTCCT GAAAGAGGAG CACGAGAAAC TCAAGCTGGC AGAGGAGCCA
    GCCCGGAGGA GCCAGAGCAG CGGCCACCGC AGCAGAAGGG GCCCAGCAGC CAGCCCGGCG
    CCCTCTGGCC GGAACCAGCC GGGCAGCAGT GCGTCCTCGG GGTCCCAGCA CACCAGCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.