RNF216 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006044283.1	XP_006044345.1	E3 ubiquitin-protein ligase RNF216 isoform X4
XM_006044280.2	XP_006044342.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_006044282.1	XP_006044344.1	E3 ubiquitin-protein ligase RNF216 isoform X3
XM_006044284.1	XP_006044346.1	E3 ubiquitin-protein ligase RNF216 isoform X5
XM_006044281.2	XP_006044343.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_025274740.1	XP_025130525.1	E3 ubiquitin-protein ligase RNF216 isoform X3
XM_025274738.1	XP_025130523.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_006044282.2	XP_006044344.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_006044283.2	XP_006044345.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_025274739.1	XP_025130524.1	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_006044280.1	XP_006044342.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_006044281.1	XP_006044343.1	E3 ubiquitin-protein ligase RNF216 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu03482	XM_006044283.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu03482	XM_006044281.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu03482	XM_006044280.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu03482	XM_006044282.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu03483	XM_006044284.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu59657	XM_006044283.2 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59657	XM_006044280.2 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59657	XM_006044281.2 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59657	XM_006044282.2 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59657	XM_025274738.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59658	XM_025274739.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu59659	XM_025274740.1 Latest version!	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

RNF216 ( XM_006044283.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044345.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu03482
Clone ID Related Accession (Same CDS sequence)	XM_006044283.1 , XM_006044282.1 , XM_006044280.1 , XM_006044281.1
Accession Version	XM_006044283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044345.1
CDS	455..3235
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044283.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044283.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044282.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044344.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu03482
Clone ID Related Accession (Same CDS sequence)	XM_006044283.1 , XM_006044282.1 , XM_006044280.1 , XM_006044281.1
Accession Version	XM_006044282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044344.1
CDS	199..2979
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044282.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044282.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044280.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044342.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu03482
Clone ID Related Accession (Same CDS sequence)	XM_006044283.1 , XM_006044282.1 , XM_006044280.1 , XM_006044281.1
Accession Version	XM_006044280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044342.1
CDS	309..3089
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044280.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044280.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044281.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044343.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu03482
Clone ID Related Accession (Same CDS sequence)	XM_006044283.1 , XM_006044282.1 , XM_006044280.1 , XM_006044281.1
Accession Version	XM_006044281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044343.1
CDS	346..3126
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044281.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044281.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCCGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044284.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044346.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu03483
Clone ID Related Accession (Same CDS sequence)	XM_006044284.1
Accession Version	XM_006044284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783599.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC CTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAA TGCTGTATCA GCATGAACCC CGGGGGTCAG CTTTTCCAGG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAGCAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GGAGCCCAAT TTGGGAAACC TTTGGGGGCA AGAAGCTTCT 
GACGTAGACC AGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAA GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGATGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGCATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA TGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCCGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCCGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGGCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAGAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCATGCA CCCGCCCTTC CCCCTGCCGC CCGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AGCACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044346.1
CDS	309..2918
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAEDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044284.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044284.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC CTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAA TGCTGTATCA GCATGAACCC CGGGGGTCAG CTTTTCCAGG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAGCAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GGAGCCCAAT TTGGGAAACC TTTGGGGGCA AGAAGCTTCT 
GACGTAGACC AGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAA GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGATGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGCATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA TGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCCGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCCGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGGCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAGAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCATGCA CCCGCCCTTC CCCCTGCCGC CCGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AGCACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044280.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044342.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu59657
Clone ID Related Accession (Same CDS sequence)	XM_006044280.2 , XM_006044281.2 , XM_025274738.1 , XM_006044282.2 , XM_006044283.2
Accession Version	XM_006044280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044342.1
CDS	322..3102
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044280.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044280.2

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044281.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044343.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu59657
Clone ID Related Accession (Same CDS sequence)	XM_006044280.2 , XM_006044281.2 , XM_025274738.1 , XM_006044282.2 , XM_006044283.2
Accession Version	XM_006044281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044281.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044343.1
CDS	349..3129
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044281.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044281.2

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_025274738.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130523.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu59657
Clone ID Related Accession (Same CDS sequence)	XM_006044280.2 , XM_006044281.2 , XM_025274738.1 , XM_006044282.2 , XM_006044283.2
Accession Version	XM_025274738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130523.1
CDS	389..3169
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_025274738.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274738.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044282.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044344.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu59657
Clone ID Related Accession (Same CDS sequence)	XM_006044280.2 , XM_006044281.2 , XM_025274738.1 , XM_006044282.2 , XM_006044283.2
Accession Version	XM_006044282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044344.1
CDS	215..2995
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044282.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044282.2

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_006044283.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044345.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu59657
Clone ID Related Accession (Same CDS sequence)	XM_006044280.2 , XM_006044281.2 , XM_025274738.1 , XM_006044282.2 , XM_006044283.2
Accession Version	XM_006044283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044345.1
CDS	467..3247
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_006044283.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044283.2

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG GAAACCTGTA GGAAGTGTCA AGGACTCTGG 
AAAGAGCACA ATGGCCTCAC TTGTGAGGAG CTGGCTGAAA AAGATGACAT CAAGTACCGA 
ACATCTATTG AAGAAAAAAT GACTGCCGCC CGCATTAGGA AATGCCACAA GTGTGGGACC 
AGCCTCATCA AATCCGAAGG CTGCAACCGC ATGTCCTGCC GCTGCGGTGC CCAGATGTGC 
TACCTCTGCC GCGTGTCCAT CAATGGCTAC GACCACTTCT GCCAGCACCC TCGCTCCCCA 
GGAGCCCCCT GCCAGGAGTG TTCCCGATGC TCTCTCTGGA CCGACCCCAC TGAAGATGAT 
GAGAAGCTAA TTGAGGAAAT CCAGAAGGAG GCCGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT CGGGCCCCCC TTGGAGAAGC CCCCTGAGAA AGTGCAGAGG 
ACCGAGGCCC TGCCGAGGCC CGTGCCCCAG AACCTGCACC AGCCGCAGAT ACCCGCCTAC 
GCCTTCATGC ACCCGCCCTT CCCCCTGCCG CCAGTCCGGC CCGTGTTCAA CAACTTCCCC 
CTCAACATGG GCCCCATCCC GGCACCCTAC GTGCCGCCCC TGCCCAATAT GCGCGTCAAC 
TACGAATTTG CCCCCATCCA CCTGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCTCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_025274739.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130524.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu59658
Clone ID Related Accession (Same CDS sequence)	XM_025274739.1
Accession Version	XM_025274739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC CTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAA TGCTGTATCA GCATGAACCC CGGGGGTCAG CTTTTCCAGG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAGCAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GGAGCCCAAT TTGGGAAACC TTTGGGGGCA AGAAGCTTCT 
GACGTAGACC AGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAA GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGATGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGCATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA TGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCCGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCCGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGGCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAGAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCATGCA CCCGCCCTTC CCCCTGCCGC CAGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AGCACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130524.1
CDS	3540..6149
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAEDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTSLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPPEKVQRTEALPRPVPQNLHQPQIPAYAFMHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNMRVNYEFAPIHLPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_025274739.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274739.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAGA TGATTCTGAG GATGACTACA GTGAATTTCT AGATCTTGGG 
CCTCCTGGAG TTTCTGGATT CATGAAGCCA AGTGGCCAAA CAGAAAGAGA ACCTAAGCCT 
GGACCAAGTC ACAACCAAGC AACCAATGAC CTCATCAACC CCAGATCAGA GCAGAAAGTC 
ATTATCTTAA AAGAAGGTAA TCTTTTTCCA GAAGCTGATC CTTTGGACAC TCAGAACCAG 
TCATCTGAAG AGTCAGAGAC AGAGCTTTTA TCAAACCTCG GAGAATCATC TGATATCCTC 
GATGACCAGG CCATCGAAGA AGACTGCTGG TTAGACCACT CTTACTTCCA GTCTCTGAAC 
CAGCAGCCCC GTGAGGTACC AAGCCAGGTT GTACCTCAGG AGCGGCGTCC AGAAGCAGAG 
CTGGGCCCAA TGCTGTATCA GCATGAACCC CGGGGGTCAG CTTTTCCAGG ACCGGCGTTT 
GCAAGACCAG AGCCCCAGCA GGATTGGATT CCAGACCCTG CTTCTCCTCG GCCTGCCCAT 
CCTCTGGGAG AGCTTGAAGA CCAGCAACTA GCAATAGATG ATGAAGAACC AGGCCCAGCA 
TTTCCGCTAG AAGAAACTCG GGAGCCCAAT TTGGGAAACC TTTGGGGGCA AGAAGCTTCT 
GACGTAGACC AGGAACTCAT TGCACTGTTG GTGAAAGAGA CAGAAGTAAG ATTTCCTGAT 
GTAGCAAATG GGTATGTTGA GGAAATAATT CATTTGAAGA ATTACTATGA TTTGAATGTA 
CTTTGTAATT TTCTTCTGGA AAATCCAGAT TATCCAAAGA GAGAAGACAG AGTCCTTATG 
AATCCCAGTA GCAGCCTGCT CGCCAGCCAA GATGAGATGA AGTTGCCTAC AGTAGACTTT 
TTTGACTATT CTAAATTAGC TCCTCTTGAC CAGCACTGCT TCATCCAAGC TGCCGACCTC 
CTGATGGCCG ACTTCAAAAT GCTTAGCAGT CAGGACATCA AGTGGGCCCT GCACGAGCTC 
AAAGGACACT ATGCAATCAC CCGAAAGGCC TTTTCTGACG CCATTAAAAA ATGGCAGGAG 
CTATCGCCAG AAACCAGTGG AAAAAGAAAA AAGAGAAAAG AAATGAATCA GTATTCTTAC 
ATAGATTTCA AATTTGAACA AGGTGACAAA AAAATAGAGA AGAGGATGTT CTTTCTGGAA 
AATAAGCGGC GACATTGTAG GTCCTATGAC CGGCGTGCCC TCCTTCCAGC TGTGCAGCAG 
GAGCAGGAGT TCTACGAGCA GAAAATCAAA GAGATGGCAG AGCATGAAGA CTTTTTGCTT 
GCCCTGCAGA TGAATGAAGA ACAGTATCAA AAGGATGGCC AGCTGATTGA GTGCCGCTGC 
TGCTATGGGG AGTTCCCATT CGAGGAGCTG ACGCAGTGTG CTGATGCTCA CTTATTCTGC 
AAAGAGTGTC TCATCAGATA CGCCCAAGAG GCAGTCTTTG GATCTGGAAA GTCAGAGCTC 
AGCTGCATGG AGGGCAGCTG CACATGCTCA TTCCCAGCCA GTGAACTGGA GAAGGTGCTT 
CCCCAGACCA TCCTGTATAA ATACTATGAG CGAAAAGCAG AGGAAGAAGT TGCAGCAGCC 
TACGCTGATG AGCTTGTCAG GTGCCCCTCC TGCAGCTTTC CTGCTCTCCT GGACAGTGAC 
GTGAAGAGGT TCAGTTGTCC CAATCCTCGC TGTCGAAAGG AAACCTGTAG GAAGTGTCAA 
GGACTCTGGA AAGAGCACAA TGGCCTCACT TGTGAGGAGC TGGCTGAAAA AGATGACATC 
AAGTACCGAA CATCTATTGA AGAAAAAATG ACTGCCGCCC GCATTAGGAA ATGCCACAAG 
TGTGGGACCA GCCTCATCAA ATCCGAAGGC TGCAACCGCA TGTCCTGCCG CTGCGGTGCC 
CAGATGTGCT ACCTCTGCCG CGTGTCCATC AATGGCTACG ACCACTTCTG CCAGCACCCT 
CGCTCCCCAG GAGCCCCCTG CCAGGAGTGT TCCCGATGCT CTCTCTGGAC CGACCCCACT 
GAAGATGATG AGAAGCTAAT TGAGGAAATC CAGAAGGAGG CCGAAGAGGA ACAGAAAAGA 
AAGAATGGAG AGAACACCTT CAAACGCATC GGGCCCCCCT TGGAGAAGCC CCCTGAGAAA 
GTGCAGAGGA CCGAGGCCCT GCCGAGGCCC GTGCCCCAGA ACCTGCACCA GCCGCAGATA 
CCCGCCTACG CCTTCATGCA CCCGCCCTTC CCCCTGCCGC CAGTCCGGCC CGTGTTCAAC 
AACTTCCCCC TCAACATGGG CCCCATCCCG GCACCCTACG TGCCGCCCCT GCCCAATATG 
CGCGTCAACT ACGAATTTGC CCCCATCCAC CTGCCCCTGG AGCACAACCT GCCCATGCAC 
TTTGGCCCCC AGCCTCGGCA TCGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_025274740.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130525.1 Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu59659
Clone ID Related Accession (Same CDS sequence)	XM_025274740.1
Accession Version	XM_025274740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG AACTGGGCAA CATGGTGGCA TCTCTGTCAC 
ATTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130525.1
CDS	3540..5648
Translation	MAEGNSNEEVIHLNNFHCHRGQDWINLRDGPITISDSSDEEGVPMLVTPAPQQHDEDLDDDVILAETYKPQTSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDTNGYFPVYNSSLFGSGTQDDSEDDYSEFLDLGPPGVSGFMKPSGQTEREPKPGPSHNQATNDLINPRSEQKVIILKEGNLFPEADPLDTQNQSSEESETELLSNLGESSDILDDQAIEEDCWLDHSYFQSLNQQPREVPSQVVPQERRPEAELGPMLYQHEPRGSAFPGPAFARPEPQQDWIPDPASPRPAHPLGELEDQQLAIDDEEPGPAFPLEETREPNLGNLWGQEASDVDQELIALLVKETEVRFPDVANGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRVLMNPSSSLLASQDEMKLPTVDFFDYSKLAPLDQHCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSYIDFKFEQGDKKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPASELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKNWATWWHLCHIW

Target ORF information:

RefSeq Version	XM_025274740.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ring finger protein 216 (RNF216), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274740.1

ORF Insert Sequence:

ATGGCAGAGG GAAACAGCAA TGAAGAGGTG ATTCACTTAA ACAATTTCCA CTGCCACCGG 
GGACAAGACT GGATCAATCT CAGAGATGGA CCCATCACCA TATCCGACTC CTCTGATGAG 
GAAGGGGTTC CAATGCTGGT CACCCCAGCT CCTCAGCAAC ATGATGAGGA CCTGGATGAT 
GATGTCATCC TGGCGGAAAC ATACAAACCT CAGACATCAC GACCCAATCT CATCAAACCA 
GCTGCCCAAT GGCAAGATCT CAAAAGATTG GGAGAAGAAA GGCCTAAAAA ATCTAGAGCA 
GCCTTTGAAT CAGATACGAA TGGCTATTTT CCCGTGTATA ACAGCTCATT GTTTGGTTCT 
GGGACACAGG ATGATTCTGA GGATGACTAC AGTGAATTTC TAGATCTTGG GCCTCCTGGA 
GTTTCTGGAT TCATGAAGCC AAGTGGCCAA ACAGAAAGAG AACCTAAGCC TGGACCAAGT 
CACAACCAAG CAACCAATGA CCTCATCAAC CCCAGATCAG AGCAGAAAGT CATTATCTTA 
AAAGAAGGTA ATCTTTTTCC AGAAGCTGAT CCTTTGGACA CTCAGAACCA GTCATCTGAA 
GAGTCAGAGA CAGAGCTTTT ATCAAACCTC GGAGAATCAT CTGATATCCT CGATGACCAG 
GCCATCGAAG AAGACTGCTG GTTAGACCAC TCTTACTTCC AGTCTCTGAA CCAGCAGCCC 
CGTGAGGTAC CAAGCCAGGT TGTACCTCAG GAGCGGCGTC CAGAAGCAGA GCTGGGCCCA 
ATGCTGTATC AGCATGAACC CCGGGGGTCA GCTTTTCCAG GACCGGCGTT TGCAAGACCA 
GAGCCCCAGC AGGATTGGAT TCCAGACCCT GCTTCTCCTC GGCCTGCCCA TCCTCTGGGA 
GAGCTTGAAG ACCAGCAACT AGCAATAGAT GATGAAGAAC CAGGCCCAGC ATTTCCGCTA 
GAAGAAACTC GGGAGCCCAA TTTGGGAAAC CTTTGGGGGC AAGAAGCTTC TGACGTAGAC 
CAGGAACTCA TTGCACTGTT GGTGAAAGAG ACAGAAGTAA GATTTCCTGA TGTAGCAAAT 
GGGTATGTTG AGGAAATAAT TCATTTGAAG AATTACTATG ATTTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAATCCAGA TTATCCAAAG AGAGAAGACA GAGTCCTTAT GAATCCCAGT 
AGCAGCCTGC TCGCCAGCCA AGATGAGATG AAGTTGCCTA CAGTAGACTT TTTTGACTAT 
TCTAAATTAG CTCCTCTTGA CCAGCACTGC TTCATCCAAG CTGCCGACCT CCTGATGGCC 
GACTTCAAAA TGCTTAGCAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTTTCTGAC GCCATTAAAA AATGGCAGGA GCTATCGCCA 
GAAACCAGTG GAAAAAGAAA AAAGAGAAAA GAAATGAATC AGTATTCTTA CATAGATTTC 
AAATTTGAAC AAGGTGACAA AAAAATAGAG AAGAGGATGT TCTTTCTGGA AAATAAGCGG 
CGACATTGTA GGTCCTATGA CCGGCGTGCC CTCCTTCCAG CTGTGCAGCA GGAGCAGGAG 
TTCTACGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTGCAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGCCGCTG CTGCTATGGG 
GAGTTCCCAT TCGAGGAGCT GACGCAGTGT GCTGATGCTC ACTTATTCTG CAAAGAGTGT 
CTCATCAGAT ACGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTCAGAGCT CAGCTGCATG 
GAGGGCAGCT GCACATGCTC ATTCCCAGCC AGTGAACTGG AGAAGGTGCT TCCCCAGACC 
ATCCTGTATA AATACTATGA GCGAAAAGCA GAGGAAGAAG TTGCAGCAGC CTACGCTGAT 
GAGCTTGTCA GGTGCCCCTC CTGCAGCTTT CCTGCTCTCC TGGACAGTGA CGTGAAGAGG 
TTCAGTTGTC CCAATCCTCG CTGTCGAAAG AACTGGGCAA CATGGTGGCA TCTCTGTCAC 
ATTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RNF216
Entrez Gene ID	102415593
Full Name	ring finger protein 216
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RNF216 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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