SSH2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following SSH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu01729 XM_006042141.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBu01730 XM_006042142.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBu62246 XM_025280956.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu62247 XM_025280957.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu62248 XM_006042141.2
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu62249 XM_025280958.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu62250 XM_025280959.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu62251 XM_025280960.1
Latest version!
Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu01729
    Clone ID Related Accession (Same CDS sequence) XM_006042141.1
    Accession Version XM_006042141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GTCTTCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006042203.1
    CDS164..4393
    Translation

    Target ORF information:

    RefSeq Version XM_006042141.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006042141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GTCTTCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu01730
    Clone ID Related Accession (Same CDS sequence) XM_006042142.1
    Accession Version XM_006042142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGTTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CTGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCAAAAGAG
    ATACGAACTG AACTGGAAAT GCAAATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAT
    GTGTTCTTGG GCTCGGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAAATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAGTTCA TCTCTAAAGC AAAGAAACAC GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTAATC GCCTATGCAA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CCAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AGCTCAACAA AAAGGAGATT ACCACCTCAG CAGATCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC AACCATCCCT CCCGTCTTTG TGGAACACGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACGTCT
    CGGGAATTTC ACGCTGGACA GATTGAAGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA TGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCAG ATCCCGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTAGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AGTCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCAATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTCGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGTC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCACCGAGA ACAGACCTGG AAACCTGGAG CAGAATGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGAGAC CCCGAGGGGT GCCCAGGCGC ACACTCCTCC
    ACCGCGGAGG TAGAAGAAGA GGAACCAGCG GAGGGGGAAC AAGAGCTCTG GGGTCCCGGG
    ATGCCTCCGG GTGCCAAGTG GTATCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAT CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCG GTGGCAGACC CGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACCGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG ATCTGCGGGA GTCTCACCCG GCGCCAGCAA CTCAGGATGG CCCACAGCAC
    GAAGGTGTCC TGCGGGAGCC GAGGACTGTG GTCTCAGGCC AGGGGCCGGA GACACCAGCG
    GCCCCTGCTC CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGT GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCTCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCACGAGCA CAGCAGGCCG GTCAACCTGC CCCCTACCCT GAGCAGCCCA
    GCAGCGCCTG TTGTCAGTCC CACGGCCCAA CCATGTGGCG CCAGCCTGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGTC TTCAGAAAAA CTGGACCCGA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTTCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCGCCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAATGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTCATAGAG CAGCTCAGAA CCACAGAGTG TATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGTACAG TATGCCAAAG AGTTCGGTTA CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGAATTG ACTAGTTCGG AAGGAGGCCT TCCTTTGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCA GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006042204.1
    CDS164..4390
    Translation

    Target ORF information:

    RefSeq Version XM_006042142.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006042142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGTTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CTGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCAAAAGAG
    ATACGAACTG AACTGGAAAT GCAAATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAT
    GTGTTCTTGG GCTCGGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAAATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAGTTCA TCTCTAAAGC AAAGAAACAC GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTAATC GCCTATGCAA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CCAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AGCTCAACAA AAAGGAGATT ACCACCTCAG CAGATCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC AACCATCCCT CCCGTCTTTG TGGAACACGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACGTCT
    CGGGAATTTC ACGCTGGACA GATTGAAGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA TGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCAG ATCCCGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTAGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AGTCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCAATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTCGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGTC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCACCGAGA ACAGACCTGG AAACCTGGAG CAGAATGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGAGAC CCCGAGGGGT GCCCAGGCGC ACACTCCTCC
    ACCGCGGAGG TAGAAGAAGA GGAACCAGCG GAGGGGGAAC AAGAGCTCTG GGGTCCCGGG
    ATGCCTCCGG GTGCCAAGTG GTATCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAT CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCG GTGGCAGACC CGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACCGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG ATCTGCGGGA GTCTCACCCG GCGCCAGCAA CTCAGGATGG CCCACAGCAC
    GAAGGTGTCC TGCGGGAGCC GAGGACTGTG GTCTCAGGCC AGGGGCCGGA GACACCAGCG
    GCCCCTGCTC CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGT GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCTCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCACGAGCA CAGCAGGCCG GTCAACCTGC CCCCTACCCT GAGCAGCCCA
    GCAGCGCCTG TTGTCAGTCC CACGGCCCAA CCATGTGGCG CCAGCCTGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGTC TTCAGAAAAA CTGGACCCGA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTTCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCGCCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAATGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTCATAGAG CAGCTCAGAA CCACAGAGTG TATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGTACAG TATGCCAAAG AGTTCGGTTA CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGAATTG ACTAGTTCGG AAGGAGGCCT TCCTTTGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCA GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62246
    Clone ID Related Accession (Same CDS sequence) XM_025280956.1
    Accession Version XM_025280956.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136741.1
    CDS1..4290
    Translation

    Target ORF information:

    RefSeq Version XM_025280956.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62247
    Clone ID Related Accession (Same CDS sequence) XM_025280957.1
    Accession Version XM_025280957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGTTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CTGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCAAAAGAG
    ATACGAACTG AACTGGAAAT GCAAATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAT
    GTGTTCTTGG GCTCGGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAAATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAGTTCA TCTCTAAAGC AAAGAAACAC GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTAATC GCCTATGCAA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CCAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AGCTCAACAA AAAGGAGATT ACCACCTCAG CAGATCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC AACCATCCCT CCCGTCTTTG TGGAACACGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACGTCT
    CGGGAATTTC ACGCTGGACA GATTGAAGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA TGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCAG ATCCCGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTAGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AGTCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCAATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTCGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGTC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCACCGAGA ACAGACCTGG AAACCTGGAG CAGAATGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGAGAC CCCGAGGGGT GCCCAGGCGC ACACTCCTCC
    ACCGCGGAGG TAGAAGAAGA GGAACCAGCG GAGGGGGAAC AAGAGCTCTG GGGTCCCGGG
    ATGCCTCCGG GTGCCAAGTG GTATCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAT CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCG GTGGCAGACC CGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACCGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG ATCTGCGGGA GTCTCACCCG GCGCCAGCAA CTCAGGATGG CCCACAGCAC
    GAAGGTGTCC TGCGGGAGCC GAGGACTGTG GTCTCAGGCC AGGGGCCGGA GACACCAGCG
    GCCCCTGCTC CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGT GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCTCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCACGAGCA CAGCAGGCCG GTCAACCTGC CCCCTACCCT GAGCAGCCCA
    GCAGCGCCTG TTGTCAGTCC CACGGCCCAA CCATGTGGCG CCAGCCTGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCGA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTTCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCGCCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAATGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTCATAGAG CAGCTCAGAA CCACAGAGTG TATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGTACAG TATGCCAAAG AGTTCGGTTA CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGAATTG ACTAGTTCGG AAGGAGGCCT TCCTTTGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCA GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136742.1
    CDS71..4357
    Translation

    Target ORF information:

    RefSeq Version XM_025280957.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGTTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CTGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCAAAAGAG
    ATACGAACTG AACTGGAAAT GCAAATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAT
    GTGTTCTTGG GCTCGGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAAATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAGTTCA TCTCTAAAGC AAAGAAACAC GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTAATC GCCTATGCAA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CCAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AGCTCAACAA AAAGGAGATT ACCACCTCAG CAGATCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC AACCATCCCT CCCGTCTTTG TGGAACACGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACGTCT
    CGGGAATTTC ACGCTGGACA GATTGAAGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA TGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCAG ATCCCGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTAGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AGTCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCAATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTCGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGTC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCACCGAGA ACAGACCTGG AAACCTGGAG CAGAATGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGAGAC CCCGAGGGGT GCCCAGGCGC ACACTCCTCC
    ACCGCGGAGG TAGAAGAAGA GGAACCAGCG GAGGGGGAAC AAGAGCTCTG GGGTCCCGGG
    ATGCCTCCGG GTGCCAAGTG GTATCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAT CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCG GTGGCAGACC CGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACCGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG ATCTGCGGGA GTCTCACCCG GCGCCAGCAA CTCAGGATGG CCCACAGCAC
    GAAGGTGTCC TGCGGGAGCC GAGGACTGTG GTCTCAGGCC AGGGGCCGGA GACACCAGCG
    GCCCCTGCTC CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGT GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCTCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCACGAGCA CAGCAGGCCG GTCAACCTGC CCCCTACCCT GAGCAGCCCA
    GCAGCGCCTG TTGTCAGTCC CACGGCCCAA CCATGTGGCG CCAGCCTGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCGA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTTCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCGCCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAATGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTCATAGAG CAGCTCAGAA CCACAGAGTG TATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGTACAG TATGCCAAAG AGTTCGGTTA CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGAATTG ACTAGTTCGG AAGGAGGCCT TCCTTTGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCA GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62248
    Clone ID Related Accession (Same CDS sequence) XM_006042141.2
    Accession Version XM_006042141.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006042203.2
    CDS126..4355
    Translation

    Target ORF information:

    RefSeq Version XM_006042141.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006042141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCTTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CGACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTTCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCGCCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA TGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTCATA GAGCAGCTCA GAACCACAGA GTGTATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGTA CAGTATGCCA AAGAGTTCGG TTACAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGAA TTGACTAGTT CGGAAGGAGG CCTTCCTTTG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCAGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62249
    Clone ID Related Accession (Same CDS sequence) XM_025280958.1
    Accession Version XM_025280958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAAGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGTTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCTGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCAAAAG AGATACGAAC TGAACTGGAA
    ATGCAAATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ATGTGTTCTT GGGCTCGGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAAA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAGTT CATCTCTAAA
    GCAAAGAAAC ACGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTAA TCGCCTATGC AATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCCAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAGCTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGATCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCAACCATCC CTCCCGTCTT TGTGGAACAC GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACGT CTCGGGAATT TCACGCTGGA
    CAGATTGAAG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AATGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC AGATCCCGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTAGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAGTCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCAAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTCG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG TCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCACCGA GAACAGACCT
    GGAAACCTGG AGCAGAATGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGAG ACCCCGAGGG GTGCCCAGGC GCACACTCCT CCACCGCGGA GGTAGAAGAA
    GAGGAACCAG CGGAGGGGGA ACAAGAGCTC TGGGGTCCCG GGATGCCTCC GGGTGCCAAG
    TGGTATCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ATCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CGGTGGCAGA CCCGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACCGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGATCTGCGG
    GAGTCTCACC CGGCGCCAGC AACTCAGGAT GGCCCACAGC ACGAAGGTGT CCTGCGGGAG
    CCGAGGACTG TGGTCTCAGG CCAGGGGCCG GAGACACCAG CGGCCCCTGC TCCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GTGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCTCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCACGAG
    CACAGCAGGC CGGTCAACCT GCCCCCTACC CTGAGCAGCC CAGCAGCGCC TGTTGTCAGT
    CCCACGGCCC AACCATGTGG CGCCAGCCTG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC GACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTTCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCGCCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAT GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTCATAG AGCAGCTCAG AACCACAGAG TGTATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGTAC AGTATGCCAA AGAGTTCGGT TACAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGAAT TGACTAGTTC GGAAGGAGGC CTTCCTTTGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CAGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136743.1
    CDS53..4261
    Translation

    Target ORF information:

    RefSeq Version XM_025280958.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAAGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGTTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCTGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCAAAAG AGATACGAAC TGAACTGGAA
    ATGCAAATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ATGTGTTCTT GGGCTCGGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAAA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAGTT CATCTCTAAA
    GCAAAGAAAC ACGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTAA TCGCCTATGC AATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCCAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAGCTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGATCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCAACCATCC CTCCCGTCTT TGTGGAACAC GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACGT CTCGGGAATT TCACGCTGGA
    CAGATTGAAG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AATGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC AGATCCCGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTAGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAGTCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCAAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTCG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG TCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCACCGA GAACAGACCT
    GGAAACCTGG AGCAGAATGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGAG ACCCCGAGGG GTGCCCAGGC GCACACTCCT CCACCGCGGA GGTAGAAGAA
    GAGGAACCAG CGGAGGGGGA ACAAGAGCTC TGGGGTCCCG GGATGCCTCC GGGTGCCAAG
    TGGTATCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ATCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CGGTGGCAGA CCCGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACCGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGATCTGCGG
    GAGTCTCACC CGGCGCCAGC AACTCAGGAT GGCCCACAGC ACGAAGGTGT CCTGCGGGAG
    CCGAGGACTG TGGTCTCAGG CCAGGGGCCG GAGACACCAG CGGCCCCTGC TCCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GTGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCTCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCACGAG
    CACAGCAGGC CGGTCAACCT GCCCCCTACC CTGAGCAGCC CAGCAGCGCC TGTTGTCAGT
    CCCACGGCCC AACCATGTGG CGCCAGCCTG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC GACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTTCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCGCCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAT GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTCATAG AGCAGCTCAG AACCACAGAG TGTATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGTAC AGTATGCCAA AGAGTTCGGT TACAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGAAT TGACTAGTTC GGAAGGAGGC CTTCCTTTGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CAGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62250
    Clone ID Related Accession (Same CDS sequence) XM_025280959.1
    Accession Version XM_025280959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGTTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCT GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CAAAAGAGAT ACGAACTGAA CTGGAAATGC AAATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCATGT GTTCTTGGGC TCGGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAAATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAGTTCATC TCTAAAGCAA AGAAACACGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTAATCGC CTATGCAATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCC
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAG CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GATCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAA CCATCCCTCC CGTCTTTGTG
    GAACACGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACGTCTCG GGAATTTCAC GCTGGACAGA TTGAAGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAATG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCAGAT
    CCCGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTAGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAG TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCAATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTCGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGTCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CACCGAGAAC AGACCTGGAA ACCTGGAGCA GAATGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGAGACCC CGAGGGGTGC
    CCAGGCGCAC ACTCCTCCAC CGCGGAGGTA GAAGAAGAGG AACCAGCGGA GGGGGAACAA
    GAGCTCTGGG GTCCCGGGAT GCCTCCGGGT GCCAAGTGGT ATCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGATCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCGGT GGCAGACCCG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACCGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAT CTGCGGGAGT CTCACCCGGC GCCAGCAACT
    CAGGATGGCC CACAGCACGA AGGTGTCCTG CGGGAGCCGA GGACTGTGGT CTCAGGCCAG
    GGGCCGGAGA CACCAGCGGC CCCTGCTCCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGTGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCTCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CACGAGCACA GCAGGCCGGT CAACCTGCCC
    CCTACCCTGA GCAGCCCAGC AGCGCCTGTT GTCAGTCCCA CGGCCCAACC ATGTGGCGCC
    AGCCTGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCGACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTTCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC GCCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAATGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TCATAGAGCA GCTCAGAACC
    ACAGAGTGTA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGTACAGTA TGCCAAAGAG
    TTCGGTTACA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGAATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTTGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCAGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136744.1
    CDS9231..13355
    Translation

    Target ORF information:

    RefSeq Version XM_025280959.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGTTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCT GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CAAAAGAGAT ACGAACTGAA CTGGAAATGC AAATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCATGT GTTCTTGGGC TCGGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAAATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAGTTCATC TCTAAAGCAA AGAAACACGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTAATCGC CTATGCAATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCC
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAG CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GATCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAA CCATCCCTCC CGTCTTTGTG
    GAACACGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACGTCTCG GGAATTTCAC GCTGGACAGA TTGAAGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAATG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCAGAT
    CCCGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTAGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAG TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCAATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTCGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGTCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CACCGAGAAC AGACCTGGAA ACCTGGAGCA GAATGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGAGACCC CGAGGGGTGC
    CCAGGCGCAC ACTCCTCCAC CGCGGAGGTA GAAGAAGAGG AACCAGCGGA GGGGGAACAA
    GAGCTCTGGG GTCCCGGGAT GCCTCCGGGT GCCAAGTGGT ATCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGATCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCGGT GGCAGACCCG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACCGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAT CTGCGGGAGT CTCACCCGGC GCCAGCAACT
    CAGGATGGCC CACAGCACGA AGGTGTCCTG CGGGAGCCGA GGACTGTGGT CTCAGGCCAG
    GGGCCGGAGA CACCAGCGGC CCCTGCTCCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGTGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCTCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CACGAGCACA GCAGGCCGGT CAACCTGCCC
    CCTACCCTGA GCAGCCCAGC AGCGCCTGTT GTCAGTCCCA CGGCCCAACC ATGTGGCGCC
    AGCCTGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCGACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTTCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC GCCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAATGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TCATAGAGCA GCTCAGAACC
    ACAGAGTGTA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGTACAGTA TGCCAAAGAG
    TTCGGTTACA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGAATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTTGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCAGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62251
    Clone ID Related Accession (Same CDS sequence) XM_025280960.1
    Accession Version XM_025280960.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein phosphatase Slingshot homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGCACCC GCCCTGGCCA TCCTCACACA GACCAAGGCC CTGTGGTCAG CCCTCATGGG
    GAAGTGGGGG CCACCTGGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136745.1
    CDS71..3754
    Translation

    Target ORF information:

    RefSeq Version XM_025280960.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTATGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAAGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGTTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCTGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCAAAA
    GAGATACGAA CTGAACTGGA AATGCAAATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CATGTGTTCT TGGGCTCGGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAA ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAGT TCATCTCTAA AGCAAAGAAA CACGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTA ATCGCCTATG CAATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCCAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAGCTCAA CAAAAAGGAG ATTACCACCT CAGCAGATCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCAACCATC CCTCCCGTCT TTGTGGAACA CGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACG
    TCTCGGGAAT TTCACGCTGG ACAGATTGAA GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAATGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CAGATCCCGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTAG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAGTCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCAA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTC GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GTCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCACCG AGAACAGACC TGGAAACCTG GAGCAGAATG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGA GACCCCGAGG GGTGCCCAGG CGCACACTCC
    TCCACCGCGG AGGTAGAAGA AGAGGAACCA GCGGAGGGGG AACAAGAGCT CTGGGGTCCC
    GGGATGCCTC CGGGTGCCAA GTGGTATCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GATCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCGGTGGCAG ACCCGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACCGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGATCTGCG GGAGTCTCAC CCGGCGCCAG CAACTCAGGA TGGCCCACAG
    CACGAAGGTG TCCTGCGGGA GCCGAGGACT GTGGTCTCAG GCCAGGGGCC GGAGACACCA
    GCGGCCCCTG CTCCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGTGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCTCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCACGA GCACAGCAGG CCGGTCAACC TGCCCCCTAC CCTGAGCAGC
    CCAGCAGCGC CTGTTGTCAG TCCCACGGCC CAACCATGTG GCGCCAGCCT GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGCACCC GCCCTGGCCA TCCTCACACA GACCAAGGCC CTGTGGTCAG CCCTCATGGG
    GAAGTGGGGG CCACCTGGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.