CILP gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006050187.1	XP_006050249.1	cartilage intermediate layer protein 1 isoform X3
XM_006050189.1	XP_006050251.1	cartilage intermediate layer protein 1 isoform X5
XM_006050191.1	XP_006050253.1	cartilage intermediate layer protein 1 isoform X7
XM_025296319.1	XP_025152104.1	cartilage intermediate layer protein 1 isoform X2
XM_006050185.1	XP_006050247.1	cartilage intermediate layer protein 1 isoform X1
XM_006050187.2	XP_006050249.2	cartilage intermediate layer protein 1 isoform X1
XM_006050186.1	XP_006050248.1	cartilage intermediate layer protein 1 isoform X2
XM_006050188.1	XP_006050250.1	cartilage intermediate layer protein 1 isoform X4
XM_006050190.1	XP_006050252.1	cartilage intermediate layer protein 1 isoform X6
XM_006050192.1	XP_006050254.1	cartilage intermediate layer protein 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu08312	XM_006050185.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050187.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050186.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050190.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050189.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050191.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08313	XM_006050188.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu08314	XM_006050192.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu45399	XM_006050187.2 Latest version!	Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu45400	XM_025296319.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

CILP ( XM_006050185.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050247.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu08312
Clone ID Related Accession (Same CDS sequence)	XM_006050185.1
Accession Version	XM_006050185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATT CCCCACATCA CCCTGCTTCC CTATGTCCCG AGAGGCTAGT AGCAGGACCC 
TGCTGGGCCC CTGTAACTGT CTTGGCTTTT CACTTCTCCT CCTGGCCCTC AGGGAGGCAG 
ATGATGCTCA CTCAGTCGGT AAGAAGAGTC CAGCCTGGGA AGAGGACCTC AGCCGTCTTT 
GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC 
CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC 
CGGGTGTGCG CTCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC 
AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG 
CAGCGACCCG GCCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC 
TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT 
GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG 
TCACTGTGCA GTGAGGCCAC CGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC 
TGTGACCTTA CCTGCCCCAT GGGCAAGGCA AATGCTGAGT GTGACGCGTG CATGTGTCAG 
GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT 
GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC 
CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT 
GCACCTATCC ACCTCACGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG 
TTCGTGAGGG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT 
GGGCAGAGCG TGTCCCTGTG TTGTAAGGCC ACAGGGAAGC CCAGACCAGA CAAGTATTTC 
TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG 
CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GACCGATGCT 
GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC 
AACCCAACCC CTGAGAGCTA CCTTATCCAG CTGCCCCATG ACTGTTTCCA GAATGCCACC 
AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GCAGCAGGAT 
AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAGGAG 
AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC 
CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGGG TCAGCGCAGC CGACAACGGG 
GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACAC GTGTGAGCAT GACCGGCTAC 
AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG 
GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT 
GCCGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCTATG 
GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG 
ATCCCATCCA AGAGTTTCTA CCGGCAGAAC GGAGAGCCCT ACATGGGAAA AGTAAAGGCC 
AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG 
AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG 
GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT 
GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAC 
CCAGACACAG GGCTGTGGGA GGAAGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC 
AGAAGGGAAG ACAGGACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT 
AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG 
AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTAT CTGTGATCAA CCTGGAGCCC 
AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT 
CCCAATGGGG CCTGCCTGCC TGCCTTCTGT GATGACCAGT CCCCTGATGC TTACTCTGCC 
TACGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC 
AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA 
GACCATGAGG ACCCACGGGT CAAGAAGACG GCTTTCCAGA TCAGCATGGC CAAGCCACGG 
CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT CCGGGAATGC 
GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGATCGGTAT 
GACTATAACA CAGTCCCTTT CAACGATGAT GACCCCATGG GCTGGACTGA AGACTACCTG 
GCATGGTGGC CCAAGCCAAT GGAGTTCAGG GCTTGCTACA TCAAGGTGAA GATTGTGGGG 
CCACTGGAGA TAAATGTGCG ATCCCGTAAC ATGGGGGGCA CCCACCGGCG GACAGTGGGA 
AGGCTGTACG GAATCCGGGA TGTGAAGAGC ACACGGGACA GGGACCAGCC CAACATCTCC 
TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG ATGCTCTATG ACCAGGACCG TGTGGACCGC 
ACGCTGGTGA AGGTCATCCC CCAGGGCAGT TGCCAACGAG CCAGTGTAAA CTCTATGCTG 
CATGAGTACC TGGTCAACCA CCTACCGCTG GCGGTCAACA ACAACACCAG TGAGTACACC 
ATGCTGGCGC CCTTGGACCC ACTGGGCCAC AACTATGGCA TCTACACTGT CACTGACCAG 
GACCCTCGCA CAGCCAAGGA GATTGCGCTC GGCCGGTGCT TTGATGGCAC ATCCGATGGC 
TCCTCCAGAG TCATGAAGGC CAATGTGGGA GTGGCCCTCA CCTTTAACTG CGTAGAGAGG 
CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC CTCCAGAACA CCCCGGCCCA GCCCTCCCCT 
GCAAGCACTG TCAGGGGAAG AGTGCCCTCA AGGAGGCAGC GGGCAAGTCA GGGTAGCCAG 
CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050247.1
CDS	124..3723
Translation	MFHSPHHPASLCPERLVAGPCWAPVTVLAFHFSSWPSGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050185.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050185.1

ORF Insert Sequence:

ATGTTCCATT CCCCACATCA CCCTGCTTCC CTATGTCCCG AGAGGCTAGT AGCAGGACCC 
TGCTGGGCCC CTGTAACTGT CTTGGCTTTT CACTTCTCCT CCTGGCCCTC AGGGAGGCAG 
ATGATGCTCA CTCAGTCGGT AAGAAGAGTC CAGCCTGGGA AGAGGACCTC AGCCGTCTTT 
GCCAAGCCTG CTAACCTCCT GGACAGTCCT GGGGAGTGGA CGATCTGGTT CAACATTGAC 
CACCCGGGTG GGCAGGGTGA CTACGAACGG CTGGATGCCA TTCAGTTCTA CTATGGGGAC 
CGGGTGTGCG CTCGGCCCCT GCGGCTAGAG GCTCGAACCA CCAACTGGAA ACCCGCAGGC 
AGCACTGGCC AAGTGGTCCA TGGCAGCCCC CTCGAGGGCT TCTGGTGCCT CAACAGGGAG 
CAGCGACCCG GCCAGAACTG CTCCAATTAC ACCGTGCGCT TCCTCTGCCC TCCAGGATCC 
TTGCGCCGAG ACACAGAGCA CAGCCTCTGG AGTCCATGGT CTCCCTGGAG CAAGTGTTCT 
GCTGCTTGTG GTCACCCTGG GGTCCAGACC CGAACACGCA CCTGCTTGGC GGAGTCGGTG 
TCACTGTGCA GTGAGGCCAC CGAGGAGGGC CGGCACTGCA TGGAACAGGC CTGTTCAGCC 
TGTGACCTTA CCTGCCCCAT GGGCAAGGCA AATGCTGAGT GTGACGCGTG CATGTGTCAG 
GACTTCGTGC TGTATGGGGC TGTCTCCCTC CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT 
GTCTACCTCC TGAGCAAGAC ACCTAAGCTA CTGACCCAGA CGGACAGCAG TGGAAGGTTC 
CGAGTCCCTG GGTTGTGCCC CGATGGCAAA AGCATCCTGA AGATCACAAA GACCAAGTTT 
GCACCTATCC ACCTCACGGT GCCTAAGACT AGACTGAAGG CAGCCACCAT CAGGGCGGAG 
TTCGTGAGGG CAGAAACGCC GTACATTGTG ATGAGCCCTG AGACAAAAGC ACGAAGAGCT 
GGGCAGAGCG TGTCCCTGTG TTGTAAGGCC ACAGGGAAGC CCAGACCAGA CAAGTATTTC 
TGGTACCATA ACAACACACT GTTGGACCCC TCCCTCTACA AGCACGACAG CAAGCTGGTG 
CTGAGGAATC TGCGGCGGGA CCAGGCCGGG GAGTACTTCT GCAAGGCCCA GACCGATGCT 
GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG ACTGTCATAG CCTCTGATGA GATTCCTTGC 
AACCCAACCC CTGAGAGCTA CCTTATCCAG CTGCCCCATG ACTGTTTCCA GAATGCCACC 
AACTCCTTCT ACTATGATGT GGGTCGTTGC CCTGTCAAGA CCTGTGCAGG GCAGCAGGAT 
AATGGGATCA GGTGCCGAGA TGCTGTGGAG CACTGCTGTG GGATCTCCAA AACAGAGGAG 
AGGGAGATCC AGTGCAGCGG GTACACGCTG CCCACCAAGG TGGCCACGGA GTGCAGCTGC 
CAGCGGTGTA CGGAGACTCG GAGAATCGTG CGGGGCCGGG TCAGCGCAGC CGACAACGGG 
GAACCCCTGC GCTTCGGCCA CGTGTACATG GGGAACACAC GTGTGAGCAT GACCGGCTAC 
AAGGGCACAT TCACTCTGCA TGTCCCTCAG GACACAGAGA GGCTGGTGCT CACGTTTGTG 
GACAGGATGC AGAAGTTCGT CAACACCACC AAAGTGCTGC CCTTCAATAA GAAGGGGAGT 
GCCGTGTTCC ATGAGATCAA GATGCTTCGG CGGAAAGAGC CCATCACCTT GGAGGCTATG 
GAGACCAACA TTATCCCCTT GGGGGATATG GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG 
ATCCCATCCA AGAGTTTCTA CCGGCAGAAC GGAGAGCCCT ACATGGGAAA AGTAAAGGCC 
AGTGTGACCT TCCTGGATCC CCGGAATATT TCCACAGCTA CTGCTGCCCA GAGTGACCTG 
AACTTCATCA ATGATGAAGG AGACACTTTC CCTCTTCGGA CATATGGCAT GTTCTCTGTG 
GACTTCAGAG ATGAAGCCAC CTCAGAGTCC CTTAATGTTG GCAAGGTGAA GGTTCATCTT 
GACTCGACCC AGGTCAAGAT GCCAGAGCAT GTGCCCATGA TGAAACTCTG GTCGCTCAAC 
CCAGACACAG GGCTGTGGGA GGAAGAAGGT GACTTCAAAC CTGAAAGCCA AAGGCGGAAC 
AGAAGGGAAG ACAGGACCTT CCTGGTGGGC AACATGGAGA TCCGTGAGAG GAGGCTTTTT 
AACCTGGATG TCCCGGAAAG CAGGAGGTGC TTCATCAAGG TGAGGGCCTA CAGGAGTGAG 
AGGTTCTTGC CCAGTGAGCA GATCCAGGGG GTTGTGGTAT CTGTGATCAA CCTGGAGCCC 
AGGACTGGCT TCTCCTCCAA CCCCAGGGCC TGGGGCCGCT TTGACAGTGT CCTCACGGGT 
CCCAATGGGG CCTGCCTGCC TGCCTTCTGT GATGACCAGT CCCCTGATGC TTACTCTGCC 
TACGTCTTGG CCAGCCTGGC TGGGGAAGAA CTGGAAGCAG TGGAGTCTTC TCCTAAATTC 
AACCCAAATG CAATTGGTGT CCCTCAGCCT TACCTCAACA AGCTCAAGTA CCGCCGGACA 
GACCATGAGG ACCCACGGGT CAAGAAGACG GCTTTCCAGA TCAGCATGGC CAAGCCACGG 
CCCAACTCAC CTGAGGAGGG CAATGGACCC ATCTATGCAT TTGAGAACCT CCGGGAATGC 
GAGGACGCAC CACCCAGTGC GGCCCATTTC CGGTTCTACC AGATTGAGGG GGATCGGTAT 
GACTATAACA CAGTCCCTTT CAACGATGAT GACCCCATGG GCTGGACTGA AGACTACCTG 
GCATGGTGGC CCAAGCCAAT GGAGTTCAGG GCTTGCTACA TCAAGGTGAA GATTGTGGGG 
CCACTGGAGA TAAATGTGCG ATCCCGTAAC ATGGGGGGCA CCCACCGGCG GACAGTGGGA 
AGGCTGTACG GAATCCGGGA TGTGAAGAGC ACACGGGACA GGGACCAGCC CAACATCTCC 
TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG ATGCTCTATG ACCAGGACCG TGTGGACCGC 
ACGCTGGTGA AGGTCATCCC CCAGGGCAGT TGCCAACGAG CCAGTGTAAA CTCTATGCTG 
CATGAGTACC TGGTCAACCA CCTACCGCTG GCGGTCAACA ACAACACCAG TGAGTACACC 
ATGCTGGCGC CCTTGGACCC ACTGGGCCAC AACTATGGCA TCTACACTGT CACTGACCAG 
GACCCTCGCA CAGCCAAGGA GATTGCGCTC GGCCGGTGCT TTGATGGCAC ATCCGATGGC 
TCCTCCAGAG TCATGAAGGC CAATGTGGGA GTGGCCCTCA CCTTTAACTG CGTAGAGAGG 
CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC CTCCAGAACA CCCCGGCCCA GCCCTCCCCT 
GCAAGCACTG TCAGGGGAAG AGTGCCCTCA AGGAGGCAGC GGGCAAGTCA GGGTAGCCAG 
CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050187.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050249.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050249.1
CDS	1592..5140
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050187.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050187.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050189.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050251.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X5, mRNA.

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CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050251.1
CDS	1671..5219
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050189.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050189.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050191.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050253.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X7, mRNA.

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CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050253.1
CDS	522..4070
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050191.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050191.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050186.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050248.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X2, mRNA.

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CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050248.1
CDS	2265..5813
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050186.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050186.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050188.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050250.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050250.1
CDS	403..3951
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050188.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050188.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050190.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050252.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu08313
Clone ID Related Accession (Same CDS sequence)	XM_006050187.1 , XM_006050189.1 , XM_006050191.1 , XM_006050186.1 , XM_006050188.1 , XM_006050190.1
Accession Version	XM_006050190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050252.1
CDS	1121..4669
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050190.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050190.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CACCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAA GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGC GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050192.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050254.1 Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X8, mRNA.

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CloneID	OBu08314
Clone ID Related Accession (Same CDS sequence)	XM_006050192.1
Accession Version	XM_006050192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCATT CCCCACATCA CCCTGCTTCC CTATGTCCCG AGAGGCTAGT AGCAGGACCC 
TGCTGGGCCC CTGTAACTGT CTTGGCTTTT CACTTCTCCT CCTGGCCCTC AGGGAGGCAG 
ATGATGCTCA CTCAGTCGGT AAGAAGAGTC CAGCCTGGGA AGAGGACCTC AGCCGTCTTT 
GCCAAGCCTG CTAACCTCCT GGACAGATCC TTGCGCCGAG ACACAGAGCA CAGCCTCTGG 
AGTCCATGGT CTCCCTGGAG CAAGTGTTCT GCTGCTTGTG GTCACCCTGG GGTCCAGACC 
CGAACACGCA CCTGCTTGGC GGAGTCGGTG TCACTGTGCA GTGAGGCCAC CGAGGAGGGC 
CGGCACTGCA TGGAACAGGC CTGTTCAGCC TGTGACCTTA CCTGCCCCAT GGGCAAGGCA 
AATGCTGAGT GTGACGCGTG CATGTGTCAG GACTTCGTGC TGTATGGGGC TGTCTCCCTC 
CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT GTCTACCTCC TGAGCAAGAC ACCTAAGCTA 
CTGACCCAGA CGGACAGCAG TGGAAGGTTC CGAGTCCCTG GGTTGTGCCC CGATGGCAAA 
AGCATCCTGA AGATCACAAA GACCAAGTTT GCACCTATCC ACCTCACGGT GCCTAAGACT 
AGACTGAAGG CAGCCACCAT CAGGGCGGAG TTCGTGAGGG CAGAAACGCC GTACATTGTG 
ATGAGCCCTG AGACAAAAGC ACGAAGAGCT GGGCAGAGCG TGTCCCTGTG TTGTAAGGCC 
ACAGGGAAGC CCAGACCAGA CAAGTATTTC TGGTACCATA ACAACACACT GTTGGACCCC 
TCCCTCTACA AGCACGACAG CAAGCTGGTG CTGAGGAATC TGCGGCGGGA CCAGGCCGGG 
GAGTACTTCT GCAAGGCCCA GACCGATGCT GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG 
ACTGTCATAG CCTCTGATGA GATTCCTTGC AACCCAACCC CTGAGAGCTA CCTTATCCAG 
CTGCCCCATG ACTGTTTCCA GAATGCCACC AACTCCTTCT ACTATGATGT GGGTCGTTGC 
CCTGTCAAGA CCTGTGCAGG GCAGCAGGAT AATGGGATCA GGTGCCGAGA TGCTGTGGAG 
CACTGCTGTG GGATCTCCAA AACAGAGGAG AGGGAGATCC AGTGCAGCGG GTACACGCTG 
CCCACCAAGG TGGCCACGGA GTGCAGCTGC CAGCGGTGTA CGGAGACTCG GAGAATCGTG 
CGGGGCCGGG TCAGCGCAGC CGACAACGGG GAACCCCTGC GCTTCGGCCA CGTGTACATG 
GGGAACACAC GTGTGAGCAT GACCGGCTAC AAGGGCACAT TCACTCTGCA TGTCCCTCAG 
GACACAGAGA GGCTGGTGCT CACGTTTGTG GACAGGATGC AGAAGTTCGT CAACACCACC 
AAAGTGCTGC CCTTCAATAA GAAGGGGAGT GCCGTGTTCC ATGAGATCAA GATGCTTCGG 
CGGAAAGAGC CCATCACCTT GGAGGCTATG GAGACCAACA TTATCCCCTT GGGGGATATG 
GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG ATCCCATCCA AGAGTTTCTA CCGGCAGAAC 
GGAGAGCCCT ACATGGGAAA AGTAAAGGCC AGTGTGACCT TCCTGGATCC CCGGAATATT 
TCCACAGCTA CTGCTGCCCA GAGTGACCTG AACTTCATCA ATGATGAAGG AGACACTTTC 
CCTCTTCGGA CATATGGCAT GTTCTCTGTG GACTTCAGAG ATGAAGCCAC CTCAGAGTCC 
CTTAATGTTG GCAAGGTGAA GGTTCATCTT GACTCGACCC AGGTCAAGAT GCCAGAGCAT 
GTGCCCATGA TGAAACTCTG GTCGCTCAAC CCAGACACAG GGCTGTGGGA GGAAGAAGGT 
GACTTCAAAC CTGAAAGCCA AAGGCGGAAC AGAAGGGAAG ACAGGACCTT CCTGGTGGGC 
AACATGGAGA TCCGTGAGAG GAGGCTTTTT AACCTGGATG TCCCGGAAAG CAGGAGGTGC 
TTCATCAAGG TGAGGGCCTA CAGGAGTGAG AGGTTCTTGC CCAGTGAGCA GATCCAGGGG 
GTTGTGGTAT CTGTGATCAA CCTGGAGCCC AGGACTGGCT TCTCCTCCAA CCCCAGGGCC 
TGGGGCCGCT TTGACAGTGT CCTCACGGGT CCCAATGGGG CCTGCCTGCC TGCCTTCTGT 
GATGACCAGT CCCCTGATGC TTACTCTGCC TACGTCTTGG CCAGCCTGGC TGGGGAAGAA 
CTGGAAGCAG TGGAGTCTTC TCCTAAATTC AACCCAAATG CAATTGGTGT CCCTCAGCCT 
TACCTCAACA AGCTCAAGTA CCGCCGGACA GACCATGAGG ACCCACGGGT CAAGAAGACG 
GCTTTCCAGA TCAGCATGGC CAAGCCACGG CCCAACTCAC CTGAGGAGGG CAATGGACCC 
ATCTATGCAT TTGAGAACCT CCGGGAATGC GAGGACGCAC CACCCAGTGC GGCCCATTTC 
CGGTTCTACC AGATTGAGGG GGATCGGTAT GACTATAACA CAGTCCCTTT CAACGATGAT 
GACCCCATGG GCTGGACTGA AGACTACCTG GCATGGTGGC CCAAGCCAAT GGAGTTCAGG 
GCTTGCTACA TCAAGGTGAA GATTGTGGGG CCACTGGAGA TAAATGTGCG ATCCCGTAAC 
ATGGGGGGCA CCCACCGGCG GACAGTGGGA AGGCTGTACG GAATCCGGGA TGTGAAGAGC 
ACACGGGACA GGGACCAGCC CAACATCTCC TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG 
ATGCTCTATG ACCAGGACCG TGTGGACCGC ACGCTGGTGA AGGTCATCCC CCAGGGCAGT 
TGCCAACGAG CCAGTGTAAA CTCTATGCTG CATGAGTACC TGGTCAACCA CCTACCGCTG 
GCGGTCAACA ACAACACCAG TGAGTACACC ATGCTGGCGC CCTTGGACCC ACTGGGCCAC 
AACTATGGCA TCTACACTGT CACTGACCAG GACCCTCGCA CAGCCAAGGA GATTGCGCTC 
GGCCGGTGCT TTGATGGCAC ATCCGATGGC TCCTCCAGAG TCATGAAGGC CAATGTGGGA 
GTGGCCCTCA CCTTTAACTG CGTAGAGAGG CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC 
CTCCAGAACA CCCCGGCCCA GCCCTCCCCT GCAAGCACTG TCAGGGGAAG AGTGCCCTCA 
AGGAGGCAGC GGGCAAGTCA GGGTAGCCAG CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT 
GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050254.1
CDS	124..3453
Translation	MFHSPHHPASLCPERLVAGPCWAPVTVLAFHFSSWPSGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDRSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQRGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050192.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050192.1

ORF Insert Sequence:

ATGTTCCATT CCCCACATCA CCCTGCTTCC CTATGTCCCG AGAGGCTAGT AGCAGGACCC 
TGCTGGGCCC CTGTAACTGT CTTGGCTTTT CACTTCTCCT CCTGGCCCTC AGGGAGGCAG 
ATGATGCTCA CTCAGTCGGT AAGAAGAGTC CAGCCTGGGA AGAGGACCTC AGCCGTCTTT 
GCCAAGCCTG CTAACCTCCT GGACAGATCC TTGCGCCGAG ACACAGAGCA CAGCCTCTGG 
AGTCCATGGT CTCCCTGGAG CAAGTGTTCT GCTGCTTGTG GTCACCCTGG GGTCCAGACC 
CGAACACGCA CCTGCTTGGC GGAGTCGGTG TCACTGTGCA GTGAGGCCAC CGAGGAGGGC 
CGGCACTGCA TGGAACAGGC CTGTTCAGCC TGTGACCTTA CCTGCCCCAT GGGCAAGGCA 
AATGCTGAGT GTGACGCGTG CATGTGTCAG GACTTCGTGC TGTATGGGGC TGTCTCCCTC 
CCTGGGGGTG CCCCAGCCTC AGGGGCTGCT GTCTACCTCC TGAGCAAGAC ACCTAAGCTA 
CTGACCCAGA CGGACAGCAG TGGAAGGTTC CGAGTCCCTG GGTTGTGCCC CGATGGCAAA 
AGCATCCTGA AGATCACAAA GACCAAGTTT GCACCTATCC ACCTCACGGT GCCTAAGACT 
AGACTGAAGG CAGCCACCAT CAGGGCGGAG TTCGTGAGGG CAGAAACGCC GTACATTGTG 
ATGAGCCCTG AGACAAAAGC ACGAAGAGCT GGGCAGAGCG TGTCCCTGTG TTGTAAGGCC 
ACAGGGAAGC CCAGACCAGA CAAGTATTTC TGGTACCATA ACAACACACT GTTGGACCCC 
TCCCTCTACA AGCACGACAG CAAGCTGGTG CTGAGGAATC TGCGGCGGGA CCAGGCCGGG 
GAGTACTTCT GCAAGGCCCA GACCGATGCT GGGGCTGCAA AGTCCCGCAT TGCCCAGCTG 
ACTGTCATAG CCTCTGATGA GATTCCTTGC AACCCAACCC CTGAGAGCTA CCTTATCCAG 
CTGCCCCATG ACTGTTTCCA GAATGCCACC AACTCCTTCT ACTATGATGT GGGTCGTTGC 
CCTGTCAAGA CCTGTGCAGG GCAGCAGGAT AATGGGATCA GGTGCCGAGA TGCTGTGGAG 
CACTGCTGTG GGATCTCCAA AACAGAGGAG AGGGAGATCC AGTGCAGCGG GTACACGCTG 
CCCACCAAGG TGGCCACGGA GTGCAGCTGC CAGCGGTGTA CGGAGACTCG GAGAATCGTG 
CGGGGCCGGG TCAGCGCAGC CGACAACGGG GAACCCCTGC GCTTCGGCCA CGTGTACATG 
GGGAACACAC GTGTGAGCAT GACCGGCTAC AAGGGCACAT TCACTCTGCA TGTCCCTCAG 
GACACAGAGA GGCTGGTGCT CACGTTTGTG GACAGGATGC AGAAGTTCGT CAACACCACC 
AAAGTGCTGC CCTTCAATAA GAAGGGGAGT GCCGTGTTCC ATGAGATCAA GATGCTTCGG 
CGGAAAGAGC CCATCACCTT GGAGGCTATG GAGACCAACA TTATCCCCTT GGGGGATATG 
GCTGGTGAAG ACCCTGTGGC TGAGCTGGAG ATCCCATCCA AGAGTTTCTA CCGGCAGAAC 
GGAGAGCCCT ACATGGGAAA AGTAAAGGCC AGTGTGACCT TCCTGGATCC CCGGAATATT 
TCCACAGCTA CTGCTGCCCA GAGTGACCTG AACTTCATCA ATGATGAAGG AGACACTTTC 
CCTCTTCGGA CATATGGCAT GTTCTCTGTG GACTTCAGAG ATGAAGCCAC CTCAGAGTCC 
CTTAATGTTG GCAAGGTGAA GGTTCATCTT GACTCGACCC AGGTCAAGAT GCCAGAGCAT 
GTGCCCATGA TGAAACTCTG GTCGCTCAAC CCAGACACAG GGCTGTGGGA GGAAGAAGGT 
GACTTCAAAC CTGAAAGCCA AAGGCGGAAC AGAAGGGAAG ACAGGACCTT CCTGGTGGGC 
AACATGGAGA TCCGTGAGAG GAGGCTTTTT AACCTGGATG TCCCGGAAAG CAGGAGGTGC 
TTCATCAAGG TGAGGGCCTA CAGGAGTGAG AGGTTCTTGC CCAGTGAGCA GATCCAGGGG 
GTTGTGGTAT CTGTGATCAA CCTGGAGCCC AGGACTGGCT TCTCCTCCAA CCCCAGGGCC 
TGGGGCCGCT TTGACAGTGT CCTCACGGGT CCCAATGGGG CCTGCCTGCC TGCCTTCTGT 
GATGACCAGT CCCCTGATGC TTACTCTGCC TACGTCTTGG CCAGCCTGGC TGGGGAAGAA 
CTGGAAGCAG TGGAGTCTTC TCCTAAATTC AACCCAAATG CAATTGGTGT CCCTCAGCCT 
TACCTCAACA AGCTCAAGTA CCGCCGGACA GACCATGAGG ACCCACGGGT CAAGAAGACG 
GCTTTCCAGA TCAGCATGGC CAAGCCACGG CCCAACTCAC CTGAGGAGGG CAATGGACCC 
ATCTATGCAT TTGAGAACCT CCGGGAATGC GAGGACGCAC CACCCAGTGC GGCCCATTTC 
CGGTTCTACC AGATTGAGGG GGATCGGTAT GACTATAACA CAGTCCCTTT CAACGATGAT 
GACCCCATGG GCTGGACTGA AGACTACCTG GCATGGTGGC CCAAGCCAAT GGAGTTCAGG 
GCTTGCTACA TCAAGGTGAA GATTGTGGGG CCACTGGAGA TAAATGTGCG ATCCCGTAAC 
ATGGGGGGCA CCCACCGGCG GACAGTGGGA AGGCTGTACG GAATCCGGGA TGTGAAGAGC 
ACACGGGACA GGGACCAGCC CAACATCTCC TCTGCCTGTT TGGAGTTCAA GTGCAGTGGG 
ATGCTCTATG ACCAGGACCG TGTGGACCGC ACGCTGGTGA AGGTCATCCC CCAGGGCAGT 
TGCCAACGAG CCAGTGTAAA CTCTATGCTG CATGAGTACC TGGTCAACCA CCTACCGCTG 
GCGGTCAACA ACAACACCAG TGAGTACACC ATGCTGGCGC CCTTGGACCC ACTGGGCCAC 
AACTATGGCA TCTACACTGT CACTGACCAG GACCCTCGCA CAGCCAAGGA GATTGCGCTC 
GGCCGGTGCT TTGATGGCAC ATCCGATGGC TCCTCCAGAG TCATGAAGGC CAATGTGGGA 
GTGGCCCTCA CCTTTAACTG CGTAGAGAGG CAGGTGGGCC GTCAGAGTGC CTTCCAGTAC 
CTCCAGAACA CCCCGGCCCA GCCCTCCCCT GCAAGCACTG TCAGGGGAAG AGTGCCCTCA 
AGGAGGCAGC GGGCAAGTCA GGGTAGCCAG CGTGGAGGGG TGGCCTCTCT GAGGTTTCCT 
GGGGCTGCTC AGCAGCCTCT GAGCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_006050187.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050249.2 Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu45399
Clone ID Related Accession (Same CDS sequence)	XM_006050187.2
Accession Version	XM_006050187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CGCCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAG GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGA GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050249.2
CDS	1598..5146
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDSPGEWTIWFNIDHPGGQGDYERLDAIQFYYGDRVCARPLRLEARTTNWKPAGSTGQVVHGSPLEGFWCLNREQRPGQNCSNYTVRFLCPPGSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNRLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQSGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_006050187.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050187.2

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGTCCTG GGGAGTGGAC GATCTGGTTC 
AACATTGACC ACCCGGGTGG GCAGGGTGAC TACGAACGGC TGGATGCCAT TCAGTTCTAC 
TATGGGGACC GGGTGTGCGC TCGGCCCCTG CGGCTAGAGG CTCGAACCAC CAACTGGAAA 
CCCGCAGGCA GCACTGGCCA AGTGGTCCAT GGCAGCCCCC TCGAGGGCTT CTGGTGCCTC 
AACAGGGAGC AGCGACCCGG CCAGAACTGC TCCAATTACA CCGTGCGCTT CCTCTGCCCT 
CCAGGATCCT TGCGCCGAGA CACAGAGCAC AGCCTCTGGA GTCCATGGTC TCCCTGGAGC 
AAGTGTTCTG CTGCTTGTGG TCACCCTGGG GTCCAGACCC GAACACGCAC CTGCTTGGCG 
GAGTCGGTGT CACTGTGCAG TGAGGCCACC GAGGAGGGCC GGCACTGCAT GGAACAGGCC 
TGTTCAGCCT GTGACCTTAC CTGCCCCATG GGCAAGGCAA ATGCTGAGTG TGACGCGTGC 
ATGTGTCAGG ACTTCGTGCT GTATGGGGCT GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA 
GGGGCTGCTG TCTACCTCCT GAGCAAGACA CCTAAGCTAC TGACCCAGAC GGACAGCAGT 
GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC GATGGCAAAA GCATCCTGAA GATCACAAAG 
ACCAAGTTTG CGCCTATCCA CCTCACGGTG CCTAAGACTA GACTGAAGGC AGCCACCATC 
AGGGCGGAGT TCGTGAGGGC AGAAACGCCG TACATTGTGA TGAGCCCTGA GACAAAAGCA 
CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT TGTAAGGCCA CAGGGAAGCC CAGACCAGAC 
AAGTATTTCT GGTACCATAA CAACACACTG TTGGACCCCT CCCTCTACAA GCACGACAGC 
AAGCTGGTGC TGAGGAATCT GCGGCGGGAC CAGGCCGGGG AGTACTTCTG CAAGGCCCAG 
ACCGATGCTG GGGCTGCAAA GTCCCGCATT GCCCAGCTGA CTGTCATAGC CTCTGATGAG 
ATTCCTTGCA ACCCAACCCC TGAGAGCTAC CTTATCCAGC TGCCCCATGA CTGTTTCCAG 
AATGCCACCA ACTCCTTCTA CTATGATGTG GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG 
CAGCAGGATA ATGGGATCAG GTGCCGAGAT GCTGTGGAGC ACTGCTGTGG GATCTCCAAA 
ACAGAGGAGA GGGAGATCCA GTGCAGCGGG TACACGCTGC CCACCAAGGT GGCCACGGAG 
TGCAGCTGCC AGCGGTGTAC GGAGACTCGG AGAATCGTGC GGGGCCGGGT CAGCGCAGCC 
GACAACGGGG AACCCCTGCG CTTCGGCCAC GTGTACATGG GGAACACACG TGTGAGCATG 
ACCGGCTACA AGGGCACATT CACTCTGCAT GTCCCTCAGG ACACAGAGAG GCTGGTGCTC 
ACGTTTGTGG ACAGGATGCA GAAGTTCGTC AACACCACCA AAGTGCTGCC CTTCAATAAG 
AAGGGGAGTG CCGTGTTCCA TGAGATCAAG ATGCTTCGGC GGAAAGAGCC CATCACCTTG 
GAGGCTATGG AGACCAACAT TATCCCCTTG GGGGATATGG CTGGTGAAGA CCCTGTGGCT 
GAGCTGGAGA TCCCATCCAA GAGTTTCTAC CGGCAGAACG GAGAGCCCTA CATGGGAAAA 
GTAAAGGCCA GTGTGACCTT CCTGGATCCC CGGAATATTT CCACAGCTAC TGCTGCCCAG 
AGTGACCTGA ACTTCATCAA TGATGAAGGA GACACTTTCC CTCTTCGGAC ATATGGCATG 
TTCTCTGTGG ACTTCAGAGA TGAAGCCACC TCAGAGTCCC TTAATGTTGG CAAGGTGAAG 
GTTCATCTTG ACTCGACCCA GGTCAAGATG CCAGAGCATG TGCCCATGAT GAAACTCTGG 
TCGCTCAACC CAGACACAGG GCTGTGGGAG GAAGAAGGTG ACTTCAAACC TGAAAGCCAA 
AGGCGGAACA GAAGGGAAGA CAGGACCTTC CTGGTGGGCA ACATGGAGAT CCGTGAGAGG 
AGGCTTTTTA ACCTGGATGT CCCGGAAAGC AGGAGGTGCT TCATCAAGGT GAGGGCCTAC 
AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG ATCCAGGGGG TTGTGGTATC TGTGATCAAC 
CTGGAGCCCA GGACTGGCTT CTCCTCCAAC CCCAGGGCCT GGGGCCGCTT TGACAGTGTC 
CTCACGGGTC CCAATGGGGC CTGCCTGCCT GCCTTCTGTG ATGACCAGTC CCCTGATGCT 
TACTCTGCCT ACGTCTTGGC CAGCCTGGCT GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT 
CCTAAATTCA ACCCAAATGC AATTGGTGTC CCTCAGCCTT ACCTCAACAG GCTCAAGTAC 
CGCCGGACAG ACCATGAGGA CCCACGGGTC AAGAAGACGG CTTTCCAGAT CAGCATGGCC 
AAGCCACGGC CCAACTCACC TGAGGAGGGC AATGGACCCA TCTATGCATT TGAGAACCTC 
CGGGAATGCG AGGACGCACC ACCCAGTGCG GCCCATTTCC GGTTCTACCA GATTGAGGGG 
GATCGGTATG ACTATAACAC AGTCCCTTTC AACGATGATG ACCCCATGGG CTGGACTGAA 
GACTACCTGG CATGGTGGCC CAAGCCAATG GAGTTCAGGG CTTGCTACAT CAAGGTGAAG 
ATTGTGGGGC CACTGGAGAT AAATGTGCGA TCCCGTAACA TGGGGGGCAC CCACCGGCGG 
ACAGTGGGAA GGCTGTACGG AATCCGGGAT GTGAAGAGCA CACGGGACAG GGACCAGCCC 
AACATCTCCT CTGCCTGTTT GGAGTTCAAG TGCAGTGGGA TGCTCTATGA CCAGGACCGT 
GTGGACCGCA CGCTGGTGAA GGTCATCCCC CAGGGCAGTT GCCAACGAGC CAGTGTAAAC 
TCTATGCTGC ATGAGTACCT GGTCAACCAC CTACCGCTGG CGGTCAACAA CAACACCAGT 
GAGTACACCA TGCTGGCGCC CTTGGACCCA CTGGGCCACA ACTATGGCAT CTACACTGTC 
ACTGACCAGG ACCCTCGCAC AGCCAAGGAG ATTGCGCTCG GCCGGTGCTT TGATGGCACA 
TCCGATGGCT CCTCCAGAGT CATGAAGGCC AATGTGGGAG TGGCCCTCAC CTTTAACTGC 
GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC TTCCAGTACC TCCAGAACAC CCCGGCCCAG 
CCCTCCCCTG CAAGCACTGT CAGGGGAAGA GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG 
GGTAGCCAGA GTGGAGGGGT GGCCTCTCTG AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG 
AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP ( XM_025296319.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025152104.1 Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu45400
Clone ID Related Accession (Same CDS sequence)	XM_025296319.1
Accession Version	XM_025296319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGATCCT TGCGCCGAGA CACAGAGCAC 
AGCCTCTGGA GTCCATGGTC TCCCTGGAGC AAGTGTTCTG CTGCTTGTGG TCACCCTGGG 
GTCCAGACCC GAACACGCAC CTGCTTGGCG GAGTCGGTGT CACTGTGCAG TGAGGCCACC 
GAGGAGGGCC GGCACTGCAT GGAACAGGCC TGTTCAGCCT GTGACCTTAC CTGCCCCATG 
GGCAAGGCAA ATGCTGAGTG TGACGCGTGC ATGTGTCAGG ACTTCGTGCT GTATGGGGCT 
GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA GGGGCTGCTG TCTACCTCCT GAGCAAGACA 
CCTAAGCTAC TGACCCAGAC GGACAGCAGT GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC 
GATGGCAAAA GCATCCTGAA GATCACAAAG ACCAAGTTTG CGCCTATCCA CCTCACGGTG 
CCTAAGACTA GACTGAAGGC AGCCACCATC AGGGCGGAGT TCGTGAGGGC AGAAACGCCG 
TACATTGTGA TGAGCCCTGA GACAAAAGCA CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT 
TGTAAGGCCA CAGGGAAGCC CAGACCAGAC AAGTATTTCT GGTACCATAA CAACACACTG 
TTGGACCCCT CCCTCTACAA GCACGACAGC AAGCTGGTGC TGAGGAATCT GCGGCGGGAC 
CAGGCCGGGG AGTACTTCTG CAAGGCCCAG ACCGATGCTG GGGCTGCAAA GTCCCGCATT 
GCCCAGCTGA CTGTCATAGC CTCTGATGAG ATTCCTTGCA ACCCAACCCC TGAGAGCTAC 
CTTATCCAGC TGCCCCATGA CTGTTTCCAG AATGCCACCA ACTCCTTCTA CTATGATGTG 
GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG CAGCAGGATA ATGGGATCAG GTGCCGAGAT 
GCTGTGGAGC ACTGCTGTGG GATCTCCAAA ACAGAGGAGA GGGAGATCCA GTGCAGCGGG 
TACACGCTGC CCACCAAGGT GGCCACGGAG TGCAGCTGCC AGCGGTGTAC GGAGACTCGG 
AGAATCGTGC GGGGCCGGGT CAGCGCAGCC GACAACGGGG AACCCCTGCG CTTCGGCCAC 
GTGTACATGG GGAACACACG TGTGAGCATG ACCGGCTACA AGGGCACATT CACTCTGCAT 
GTCCCTCAGG ACACAGAGAG GCTGGTGCTC ACGTTTGTGG ACAGGATGCA GAAGTTCGTC 
AACACCACCA AAGTGCTGCC CTTCAATAAG AAGGGGAGTG CCGTGTTCCA TGAGATCAAG 
ATGCTTCGGC GGAAAGAGCC CATCACCTTG GAGGCTATGG AGACCAACAT TATCCCCTTG 
GGGGATATGG CTGGTGAAGA CCCTGTGGCT GAGCTGGAGA TCCCATCCAA GAGTTTCTAC 
CGGCAGAACG GAGAGCCCTA CATGGGAAAA GTAAAGGCCA GTGTGACCTT CCTGGATCCC 
CGGAATATTT CCACAGCTAC TGCTGCCCAG AGTGACCTGA ACTTCATCAA TGATGAAGGA 
GACACTTTCC CTCTTCGGAC ATATGGCATG TTCTCTGTGG ACTTCAGAGA TGAAGCCACC 
TCAGAGTCCC TTAATGTTGG CAAGGTGAAG GTTCATCTTG ACTCGACCCA GGTCAAGATG 
CCAGAGCATG TGCCCATGAT GAAACTCTGG TCGCTCAACC CAGACACAGG GCTGTGGGAG 
GAAGAAGGTG ACTTCAAACC TGAAAGCCAA AGGCGGAACA GAAGGGAAGA CAGGACCTTC 
CTGGTGGGCA ACATGGAGAT CCGTGAGAGG AGGCTTTTTA ACCTGGATGT CCCGGAAAGC 
AGGAGGTGCT TCATCAAGGT GAGGGCCTAC AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG 
ATCCAGGGGG TTGTGGTATC TGTGATCAAC CTGGAGCCCA GGACTGGCTT CTCCTCCAAC 
CCCAGGGCCT GGGGCCGCTT TGACAGTGTC CTCACGGGTC CCAATGGGGC CTGCCTGCCT 
GCCTTCTGTG ATGACCAGTC CCCTGATGCT TACTCTGCCT ACGTCTTGGC CAGCCTGGCT 
GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT CCTAAATTCA ACCCAAATGC AATTGGTGTC 
CCTCAGCCTT ACCTCAACAG GCTCAAGTAC CGCCGGACAG ACCATGAGGA CCCACGGGTC 
AAGAAGACGG CTTTCCAGAT CAGCATGGCC AAGCCACGGC CCAACTCACC TGAGGAGGGC 
AATGGACCCA TCTATGCATT TGAGAACCTC CGGGAATGCG AGGACGCACC ACCCAGTGCG 
GCCCATTTCC GGTTCTACCA GATTGAGGGG GATCGGTATG ACTATAACAC AGTCCCTTTC 
AACGATGATG ACCCCATGGG CTGGACTGAA GACTACCTGG CATGGTGGCC CAAGCCAATG 
GAGTTCAGGG CTTGCTACAT CAAGGTGAAG ATTGTGGGGC CACTGGAGAT AAATGTGCGA 
TCCCGTAACA TGGGGGGCAC CCACCGGCGG ACAGTGGGAA GGCTGTACGG AATCCGGGAT 
GTGAAGAGCA CACGGGACAG GGACCAGCCC AACATCTCCT CTGCCTGTTT GGAGTTCAAG 
TGCAGTGGGA TGCTCTATGA CCAGGACCGT GTGGACCGCA CGCTGGTGAA GGTCATCCCC 
CAGGGCAGTT GCCAACGAGC CAGTGTAAAC TCTATGCTGC ATGAGTACCT GGTCAACCAC 
CTACCGCTGG CGGTCAACAA CAACACCAGT GAGTACACCA TGCTGGCGCC CTTGGACCCA 
CTGGGCCACA ACTATGGCAT CTACACTGTC ACTGACCAGG ACCCTCGCAC AGCCAAGGAG 
ATTGCGCTCG GCCGGTGCTT TGATGGCACA TCCGATGGCT CCTCCAGAGT CATGAAGGCC 
AATGTGGGAG TGGCCCTCAC CTTTAACTGC GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC 
TTCCAGTACC TCCAGAACAC CCCGGCCCAG CCCTCCCCTG CAAGCACTGT CAGGGGAAGA 
GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG GGTAGCCAGA GTGGAGGGGT GGCCTCTCTG 
AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025152104.1
CDS	1598..4876
Translation	MAGAKACTFFFLVLEITSALGRQMMLTQSVRRVQPGKRTSAVFAKPANLLDRSLRRDTEHSLWSPWSPWSKCSAACGHPGVQTRTRTCLAESVSLCSEATEEGRHCMEQACSACDLTCPMGKANAECDACMCQDFVLYGAVSLPGGAPASGAAVYLLSKTPKLLTQTDSSGRFRVPGLCPDGKSILKITKTKFAPIHLTVPKTRLKAATIRAEFVRAETPYIVMSPETKARRAGQSVSLCCKATGKPRPDKYFWYHNNTLLDPSLYKHDSKLVLRNLRRDQAGEYFCKAQTDAGAAKSRIAQLTVIASDEIPCNPTPESYLIQLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVEHCCGISKTEEREIQCSGYTLPTKVATECSCQRCTETRRIVRGRVSAADNGEPLRFGHVYMGNTRVSMTGYKGTFTLHVPQDTERLVLTFVDRMQKFVNTTKVLPFNKKGSAVFHEIKMLRRKEPITLEAMETNIIPLGDMAGEDPVAELEIPSKSFYRQNGEPYMGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNVGKVKVHLDSTQVKMPEHVPMMKLWSLNPDTGLWEEEGDFKPESQRRNRREDRTFLVGNMEIRERRLFNLDVPESRRCFIKVRAYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVLTGPNGACLPAFCDDQSPDAYSAYVLASLAGEELEAVESSPKFNPNAIGVPQPYLNRLKYRRTDHEDPRVKKTAFQISMAKPRPNSPEEGNGPIYAFENLRECEDAPPSAAHFRFYQIEGDRYDYNTVPFNDDDPMGWTEDYLAWWPKPMEFRACYIKVKIVGPLEINVRSRNMGGTHRRTVGRLYGIRDVKSTRDRDQPNISSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCQRASVNSMLHEYLVNHLPLAVNNNTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRVMKANVGVALTFNCVERQVGRQSAFQYLQNTPAQPSPASTVRGRVPSRRQRASQGSQSGGVASLRFPGAAQQPLSD

Target ORF information:

RefSeq Version	XM_025296319.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein (CILP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025296319.1

ORF Insert Sequence:

ATGGCAGGGG CGAAGGCCTG CACGTTCTTC TTCCTAGTTC TGGAGATCAC CTCAGCGTTG 
GGGAGGCAGA TGATGCTCAC TCAGTCGGTA AGAAGAGTCC AGCCTGGGAA GAGGACCTCA 
GCCGTCTTTG CCAAGCCTGC TAACCTCCTG GACAGATCCT TGCGCCGAGA CACAGAGCAC 
AGCCTCTGGA GTCCATGGTC TCCCTGGAGC AAGTGTTCTG CTGCTTGTGG TCACCCTGGG 
GTCCAGACCC GAACACGCAC CTGCTTGGCG GAGTCGGTGT CACTGTGCAG TGAGGCCACC 
GAGGAGGGCC GGCACTGCAT GGAACAGGCC TGTTCAGCCT GTGACCTTAC CTGCCCCATG 
GGCAAGGCAA ATGCTGAGTG TGACGCGTGC ATGTGTCAGG ACTTCGTGCT GTATGGGGCT 
GTCTCCCTCC CTGGGGGTGC CCCAGCCTCA GGGGCTGCTG TCTACCTCCT GAGCAAGACA 
CCTAAGCTAC TGACCCAGAC GGACAGCAGT GGAAGGTTCC GAGTCCCTGG GTTGTGCCCC 
GATGGCAAAA GCATCCTGAA GATCACAAAG ACCAAGTTTG CGCCTATCCA CCTCACGGTG 
CCTAAGACTA GACTGAAGGC AGCCACCATC AGGGCGGAGT TCGTGAGGGC AGAAACGCCG 
TACATTGTGA TGAGCCCTGA GACAAAAGCA CGAAGAGCTG GGCAGAGCGT GTCCCTGTGT 
TGTAAGGCCA CAGGGAAGCC CAGACCAGAC AAGTATTTCT GGTACCATAA CAACACACTG 
TTGGACCCCT CCCTCTACAA GCACGACAGC AAGCTGGTGC TGAGGAATCT GCGGCGGGAC 
CAGGCCGGGG AGTACTTCTG CAAGGCCCAG ACCGATGCTG GGGCTGCAAA GTCCCGCATT 
GCCCAGCTGA CTGTCATAGC CTCTGATGAG ATTCCTTGCA ACCCAACCCC TGAGAGCTAC 
CTTATCCAGC TGCCCCATGA CTGTTTCCAG AATGCCACCA ACTCCTTCTA CTATGATGTG 
GGTCGTTGCC CTGTCAAGAC CTGTGCAGGG CAGCAGGATA ATGGGATCAG GTGCCGAGAT 
GCTGTGGAGC ACTGCTGTGG GATCTCCAAA ACAGAGGAGA GGGAGATCCA GTGCAGCGGG 
TACACGCTGC CCACCAAGGT GGCCACGGAG TGCAGCTGCC AGCGGTGTAC GGAGACTCGG 
AGAATCGTGC GGGGCCGGGT CAGCGCAGCC GACAACGGGG AACCCCTGCG CTTCGGCCAC 
GTGTACATGG GGAACACACG TGTGAGCATG ACCGGCTACA AGGGCACATT CACTCTGCAT 
GTCCCTCAGG ACACAGAGAG GCTGGTGCTC ACGTTTGTGG ACAGGATGCA GAAGTTCGTC 
AACACCACCA AAGTGCTGCC CTTCAATAAG AAGGGGAGTG CCGTGTTCCA TGAGATCAAG 
ATGCTTCGGC GGAAAGAGCC CATCACCTTG GAGGCTATGG AGACCAACAT TATCCCCTTG 
GGGGATATGG CTGGTGAAGA CCCTGTGGCT GAGCTGGAGA TCCCATCCAA GAGTTTCTAC 
CGGCAGAACG GAGAGCCCTA CATGGGAAAA GTAAAGGCCA GTGTGACCTT CCTGGATCCC 
CGGAATATTT CCACAGCTAC TGCTGCCCAG AGTGACCTGA ACTTCATCAA TGATGAAGGA 
GACACTTTCC CTCTTCGGAC ATATGGCATG TTCTCTGTGG ACTTCAGAGA TGAAGCCACC 
TCAGAGTCCC TTAATGTTGG CAAGGTGAAG GTTCATCTTG ACTCGACCCA GGTCAAGATG 
CCAGAGCATG TGCCCATGAT GAAACTCTGG TCGCTCAACC CAGACACAGG GCTGTGGGAG 
GAAGAAGGTG ACTTCAAACC TGAAAGCCAA AGGCGGAACA GAAGGGAAGA CAGGACCTTC 
CTGGTGGGCA ACATGGAGAT CCGTGAGAGG AGGCTTTTTA ACCTGGATGT CCCGGAAAGC 
AGGAGGTGCT TCATCAAGGT GAGGGCCTAC AGGAGTGAGA GGTTCTTGCC CAGTGAGCAG 
ATCCAGGGGG TTGTGGTATC TGTGATCAAC CTGGAGCCCA GGACTGGCTT CTCCTCCAAC 
CCCAGGGCCT GGGGCCGCTT TGACAGTGTC CTCACGGGTC CCAATGGGGC CTGCCTGCCT 
GCCTTCTGTG ATGACCAGTC CCCTGATGCT TACTCTGCCT ACGTCTTGGC CAGCCTGGCT 
GGGGAAGAAC TGGAAGCAGT GGAGTCTTCT CCTAAATTCA ACCCAAATGC AATTGGTGTC 
CCTCAGCCTT ACCTCAACAG GCTCAAGTAC CGCCGGACAG ACCATGAGGA CCCACGGGTC 
AAGAAGACGG CTTTCCAGAT CAGCATGGCC AAGCCACGGC CCAACTCACC TGAGGAGGGC 
AATGGACCCA TCTATGCATT TGAGAACCTC CGGGAATGCG AGGACGCACC ACCCAGTGCG 
GCCCATTTCC GGTTCTACCA GATTGAGGGG GATCGGTATG ACTATAACAC AGTCCCTTTC 
AACGATGATG ACCCCATGGG CTGGACTGAA GACTACCTGG CATGGTGGCC CAAGCCAATG 
GAGTTCAGGG CTTGCTACAT CAAGGTGAAG ATTGTGGGGC CACTGGAGAT AAATGTGCGA 
TCCCGTAACA TGGGGGGCAC CCACCGGCGG ACAGTGGGAA GGCTGTACGG AATCCGGGAT 
GTGAAGAGCA CACGGGACAG GGACCAGCCC AACATCTCCT CTGCCTGTTT GGAGTTCAAG 
TGCAGTGGGA TGCTCTATGA CCAGGACCGT GTGGACCGCA CGCTGGTGAA GGTCATCCCC 
CAGGGCAGTT GCCAACGAGC CAGTGTAAAC TCTATGCTGC ATGAGTACCT GGTCAACCAC 
CTACCGCTGG CGGTCAACAA CAACACCAGT GAGTACACCA TGCTGGCGCC CTTGGACCCA 
CTGGGCCACA ACTATGGCAT CTACACTGTC ACTGACCAGG ACCCTCGCAC AGCCAAGGAG 
ATTGCGCTCG GCCGGTGCTT TGATGGCACA TCCGATGGCT CCTCCAGAGT CATGAAGGCC 
AATGTGGGAG TGGCCCTCAC CTTTAACTGC GTAGAGAGGC AGGTGGGCCG TCAGAGTGCC 
TTCCAGTACC TCCAGAACAC CCCGGCCCAG CCCTCCCCTG CAAGCACTGT CAGGGGAAGA 
GTGCCCTCAA GGAGGCAGCG GGCAAGTCAG GGTAGCCAGA GTGGAGGGGT GGCCTCTCTG 
AGGTTTCCTG GGGCTGCTCA GCAGCCTCTG AGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CILP
Entrez Gene ID	102415319
Full Name	cartilage intermediate layer protein
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CILP cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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