LOC102415279 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102415279
Entrez Gene ID	102415279
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase family member 6
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006043250.1	XP_006043312.1	probable E3 ubiquitin-protein ligase HERC6 isoform X1
XM_006043251.1	XP_006043313.1	probable E3 ubiquitin-protein ligase HERC6 isoform X2
XM_025290428.1	XP_025146213.1	probable E3 ubiquitin-protein ligase HERC6 isoform X1
XM_006043251.2	XP_006043313.1	probable E3 ubiquitin-protein ligase HERC6 isoform X4
XM_006043252.1	XP_006043314.1	probable E3 ubiquitin-protein ligase HERC6 isoform X3
XM_025290429.1	XP_025146214.1	probable E3 ubiquitin-protein ligase HERC6 isoform X2
XM_006043250.2	XP_006043312.1	probable E3 ubiquitin-protein ligase HERC6 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC102415279 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102415279 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu02635	XM_006043252.1 Latest version!	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu02633	XM_006043250.2 Latest version!	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu02634	XM_006043251.2 Latest version!	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu70196	XM_025290428.1 Latest version!	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu70197	XM_025290429.1 Latest version!	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OBu02634	XM_006043251.1	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu02633	XM_006043250.1	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102415279 ( XM_006043252.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006043314.1 Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu02635
Clone ID Related Accession (Same CDS sequence)	XM_006043252.1
Accession Version	XM_006043252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783330.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGAAATCT GAAGTGAACA ACTACATGGA TTTTAGGGGA 
ATAATTCTGC CAAGAAGGGA TGAATCTCCT TCATTTACGC TTAGAGTCAG ACGAAGTCAC 
TTAGTTGAAG ATGCTCTATG TCAGTTAAGC CAAGCTGAAG ACACTGACCT CCAGAAAATA 
TTAGTGGTTG AATTTATTAA AGAAATTCGT TCTGTGGGGG AAGGGGTGAA GTCTGAGTTT 
TTTCACTGTA TATTTGAATC TATGACCAAG GAAGAATATG GAATGTTCAT ATATCCCGAG 
GAGGATTCCT ACATGTGGTT TCCTGTCAAA CCTAAACTTG AGAAGAAGAT GTATTTCCTC 
TTTGGGATGC TGTGTGGACT CTCCCTATAT AATTTTAATG TTGTCTACCT CCCTTTCCCA 
CTGGCTCTGT TTAAGAAACT TCTGAACCAG GAGCCATCAT TGGAAGATTT AAAAGAACTC 
AGTCCTTCTT TTGGAAAGTG TTTGCAAGAA GTTCTAAATG ATGATGCTAA TGACATTAAA 
GAAGAATTTG GCCTACGTTT TTCTATACAC TGGGACCAAA ATGATGTTGA TTTAATTCCA 
AATGGGATTT CTGTATTTGT AGACCAAAGC AACAAGAAAG ACTATGTTTC TAAGTGTGTT 
GATTATGTTT TCAACACCTC TGTAAAGGCA GTTTATGAGG AATTTCAGAG AGGATTTTAT 
AAACTTTTTG ACAAAGAGAT ACTTAAACAT TTCAAGCCTG AAGAACTAAT GAGAGCAATA 
ATTGGAAATA CTGACTATGA CTGGGAACAA TTTGAAAAGA ATTCTATATA TGAGCAAGGA 
TACCATGAGT CACATCCTAC TATACTGATG TTTTGGAAAG CTTTCCATAA TTTAACTTTG 
GATGAAAAGA GAAAATTCCT CTTTTTTCTT ACAGGAAATG ATAGACTACA TGTAAAAGGC 
ATACAGAAAC CAGGAATCCG GTTTTGCTGT CCTGAGACTT GCAGTGAAAG CGATTTCCCA 
AAATCACTGA CATGTCATAA TATTCTGGAA CTCCCTAAAT ATTCTACAAT GGAAAAAGTG 
AAGGAAGCAC TTCAAATAGT CATCAACAGC AACAAAGGAT TTATCTCAGC CACAGTCACA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006043314.1
CDS	349..3294
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_006043252.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006043252.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGAAATCT GAAGTGAACA ACTACATGGA TTTTAGGGGA 
ATAATTCTGC CAAGAAGGGA TGAATCTCCT TCATTTACGC TTAGAGTCAG ACGAAGTCAC 
TTAGTTGAAG ATGCTCTATG TCAGTTAAGC CAAGCTGAAG ACACTGACCT CCAGAAAATA 
TTAGTGGTTG AATTTATTAA AGAAATTCGT TCTGTGGGGG AAGGGGTGAA GTCTGAGTTT 
TTTCACTGTA TATTTGAATC TATGACCAAG GAAGAATATG GAATGTTCAT ATATCCCGAG 
GAGGATTCCT ACATGTGGTT TCCTGTCAAA CCTAAACTTG AGAAGAAGAT GTATTTCCTC 
TTTGGGATGC TGTGTGGACT CTCCCTATAT AATTTTAATG TTGTCTACCT CCCTTTCCCA 
CTGGCTCTGT TTAAGAAACT TCTGAACCAG GAGCCATCAT TGGAAGATTT AAAAGAACTC 
AGTCCTTCTT TTGGAAAGTG TTTGCAAGAA GTTCTAAATG ATGATGCTAA TGACATTAAA 
GAAGAATTTG GCCTACGTTT TTCTATACAC TGGGACCAAA ATGATGTTGA TTTAATTCCA 
AATGGGATTT CTGTATTTGT AGACCAAAGC AACAAGAAAG ACTATGTTTC TAAGTGTGTT 
GATTATGTTT TCAACACCTC TGTAAAGGCA GTTTATGAGG AATTTCAGAG AGGATTTTAT 
AAACTTTTTG ACAAAGAGAT ACTTAAACAT TTCAAGCCTG AAGAACTAAT GAGAGCAATA 
ATTGGAAATA CTGACTATGA CTGGGAACAA TTTGAAAAGA ATTCTATATA TGAGCAAGGA 
TACCATGAGT CACATCCTAC TATACTGATG TTTTGGAAAG CTTTCCATAA TTTAACTTTG 
GATGAAAAGA GAAAATTCCT CTTTTTTCTT ACAGGAAATG ATAGACTACA TGTAAAAGGC 
ATACAGAAAC CAGGAATCCG GTTTTGCTGT CCTGAGACTT GCAGTGAAAG CGATTTCCCA 
AAATCACTGA CATGTCATAA TATTCTGGAA CTCCCTAAAT ATTCTACAAT GGAAAAAGTG 
AAGGAAGCAC TTCAAATAGT CATCAACAGC AACAAAGGAT TTATCTCAGC CACAGTCACA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_006043250.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006043312.1 Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu02633
Clone ID Related Accession (Same CDS sequence)	XM_006043250.1 , XM_006043250.2
Accession Version	XM_006043250.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783330.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGTGAAAT TAGAGTGATA TTTTCATCTC CTGCTTGTCT GACTGCAAGT 
TTTTTAAAGA AGAGAGAACC TGGAGAAATT ATTTCCATTG ATGTGGACTT AGAAATGGCA 
AGAAATACCT TCAAGAAGTT AACAGAAAAA GAATGGATTT CTTCGATGAT AACCACATGT 
CTCAAGGATA ATCTGCTTGG GGCTCTTCCA TGTCGTTCTT CACACCAAGA AGCTTTATCA 
GTCTTCCTTC TGCTCCCAGA GTGTCCTGTC ATGCTTGATT CCAGGAACTG TATAACTCTG 
GTGGTTCCAT TTGCAGAGAC TATTCGTAAA ATGACCAATC AATCTTCAAA AGTCCTGAAG 
CAGTGTTGGA CATCTTTGCA AGAATCTTCT CTCAACTCAC TTGTCCAGAT GCTGAAAACT 
GCCATCATTT CTCAAATGAT TTGCTGGACT GAAACTGTTC AGAGTAACCA CAATGTTAAA 
ACTCTTCTGG AAGTGATGAA AGACGTGTAT AAGGTAAACA AAACTAACTG TCGATTACCA 
GAAGATATGT TCCACATAAA TGAATTCTCC TTTTTGTTGG ACTTTTATGA AGATAGAAGA 
AGAGTGATCA ATAGGGAAAA AAACCTGATA CCTGCAGAAA ACTTCAATCT GATTATTTTC 
AGTGATTTTC CATTTGCCTT TAATTTGGTG TCCAAAATTA AATTATTGCA AGCTGATTCC 
CAAATAAGAA TGCTGAAATC TGAAGTGAAC AACTACATGG ATTTTAGGGG AATAATTCTG 
CCAAGAAGGG ATGAATCTCC TTCATTTACG CTTAGAGTCA GACGAAGTCA CTTAGTTGAA 
GATGCTCTAT GTCAGTTAAG CCAAGCTGAA GACACTGACC TCCAGAAAAT ATTAGTGGTT 
GAATTTATTA AAGAAATTCG TTCTGTGGGG GAAGGGGTGA AGTCTGAGTT TTTTCACTGT 
ATATTTGAAT CTATGACCAA GGAAGAATAT GGAATGTTCA TATATCCCGA GGAGGATTCC 
TACATGTGGT TTCCTGTCAA ACCTAAACTT GAGAAGAAGA TGTATTTCCT CTTTGGGATG 
CTGTGTGGAC TCTCCCTATA TAATTTTAAT GTTGTCTACC TCCCTTTCCC ACTGGCTCTG 
TTTAAGAAAC TTCTGAACCA GGAGCCATCA TTGGAAGATT TAAAAGAACT CAGTCCTTCT 
TTTGGAAAGT GTTTGCAAGA AGTTCTAAAT GATGATGCTA ATGACATTAA AGAAGAATTT 
GGCCTACGTT TTTCTATACA CTGGGACCAA AATGATGTTG ATTTAATTCC AAATGGGATT 
TCTGTATTTG TAGACCAAAG CAACAAGAAA GACTATGTTT CTAAGTGTGT TGATTATGTT 
TTCAACACCT CTGTAAAGGC AGTTTATGAG GAATTTCAGA GAGGATTTTA TAAACTTTTT 
GACAAAGAGA TACTTAAACA TTTCAAGCCT GAAGAACTAA TGAGAGCAAT AATTGGAAAT 
ACTGACTATG ACTGGGAACA ATTTGAAAAG AATTCTATAT ATGAGCAAGG ATACCATGAG 
TCACATCCTA CTATACTGAT GTTTTGGAAA GCTTTCCATA ATTTAACTTT GGATGAAAAG 
AGAAAATTCC TCTTTTTTCT TACAGGAAAT GATAGACTAC ATGTAAAAGG CATACAGAAA 
CCAGGAATCC GGTTTTGCTG TCCTGAGACT TGCAGTGAAA GCGATTTCCC AAAATCACTG 
ACATGTCATA ATATTCTGGA ACTCCCTAAA TATTCTACAA TGGAAAAAGT GAAGGAAGCA 
CTTCAAATAG TCATCAACAG CAACAAAGGA TTTATCTCAG CCACAGTCAC AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006043312.1
CDS	347..3403
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQKDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_006043250.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006043250.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGTGAAAT TAGAGTGATA TTTTCATCTC CTGCTTGTCT GACTGCAAGT 
TTTTTAAAGA AGAGAGAACC TGGAGAAATT ATTTCCATTG ATGTGGACTT AGAAATGGCA 
AGAAATACCT TCAAGAAGTT AACAGAAAAA GAATGGATTT CTTCGATGAT AACCACATGT 
CTCAAGGATA ATCTGCTTGG GGCTCTTCCA TGTCGTTCTT CACACCAAGA AGCTTTATCA 
GTCTTCCTTC TGCTCCCAGA GTGTCCTGTC ATGCTTGATT CCAGGAACTG TATAACTCTG 
GTGGTTCCAT TTGCAGAGAC TATTCGTAAA ATGACCAATC AATCTTCAAA AGTCCTGAAG 
CAGTGTTGGA CATCTTTGCA AGAATCTTCT CTCAACTCAC TTGTCCAGAT GCTGAAAACT 
GCCATCATTT CTCAAATGAT TTGCTGGACT GAAACTGTTC AGAGTAACCA CAATGTTAAA 
ACTCTTCTGG AAGTGATGAA AGACGTGTAT AAGGTAAACA AAACTAACTG TCGATTACCA 
GAAGATATGT TCCACATAAA TGAATTCTCC TTTTTGTTGG ACTTTTATGA AGATAGAAGA 
AGAGTGATCA ATAGGGAAAA AAACCTGATA CCTGCAGAAA ACTTCAATCT GATTATTTTC 
AGTGATTTTC CATTTGCCTT TAATTTGGTG TCCAAAATTA AATTATTGCA AGCTGATTCC 
CAAATAAGAA TGCTGAAATC TGAAGTGAAC AACTACATGG ATTTTAGGGG AATAATTCTG 
CCAAGAAGGG ATGAATCTCC TTCATTTACG CTTAGAGTCA GACGAAGTCA CTTAGTTGAA 
GATGCTCTAT GTCAGTTAAG CCAAGCTGAA GACACTGACC TCCAGAAAAT ATTAGTGGTT 
GAATTTATTA AAGAAATTCG TTCTGTGGGG GAAGGGGTGA AGTCTGAGTT TTTTCACTGT 
ATATTTGAAT CTATGACCAA GGAAGAATAT GGAATGTTCA TATATCCCGA GGAGGATTCC 
TACATGTGGT TTCCTGTCAA ACCTAAACTT GAGAAGAAGA TGTATTTCCT CTTTGGGATG 
CTGTGTGGAC TCTCCCTATA TAATTTTAAT GTTGTCTACC TCCCTTTCCC ACTGGCTCTG 
TTTAAGAAAC TTCTGAACCA GGAGCCATCA TTGGAAGATT TAAAAGAACT CAGTCCTTCT 
TTTGGAAAGT GTTTGCAAGA AGTTCTAAAT GATGATGCTA ATGACATTAA AGAAGAATTT 
GGCCTACGTT TTTCTATACA CTGGGACCAA AATGATGTTG ATTTAATTCC AAATGGGATT 
TCTGTATTTG TAGACCAAAG CAACAAGAAA GACTATGTTT CTAAGTGTGT TGATTATGTT 
TTCAACACCT CTGTAAAGGC AGTTTATGAG GAATTTCAGA GAGGATTTTA TAAACTTTTT 
GACAAAGAGA TACTTAAACA TTTCAAGCCT GAAGAACTAA TGAGAGCAAT AATTGGAAAT 
ACTGACTATG ACTGGGAACA ATTTGAAAAG AATTCTATAT ATGAGCAAGG ATACCATGAG 
TCACATCCTA CTATACTGAT GTTTTGGAAA GCTTTCCATA ATTTAACTTT GGATGAAAAG 
AGAAAATTCC TCTTTTTTCT TACAGGAAAT GATAGACTAC ATGTAAAAGG CATACAGAAA 
CCAGGAATCC GGTTTTGCTG TCCTGAGACT TGCAGTGAAA GCGATTTCCC AAAATCACTG 
ACATGTCATA ATATTCTGGA ACTCCCTAAA TATTCTACAA TGGAAAAAGT GAAGGAAGCA 
CTTCAAATAG TCATCAACAG CAACAAAGGA TTTATCTCAG CCACAGTCAC AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_006043250.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006043312.1 Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu02633
Clone ID Related Accession (Same CDS sequence)	XM_006043250.1 , XM_006043250.2
Accession Version	XM_006043250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGTGAAAT TAGAGTGATA TTTTCATCTC CTGCTTGTCT GACTGCAAGT 
TTTTTAAAGA AGAGAGAACC TGGAGAAATT ATTTCCATTG ATGTGGACTT AGAAATGGCA 
AGAAATACCT TCAAGAAGTT AACAGAAAAA GAATGGATTT CTTCGATGAT AACCACATGT 
CTCAAGGATA ATCTGCTTGG GGCTCTTCCA TGTCGTTCTT CACACCAAGA AGCTTTATCA 
GTCTTCCTTC TGCTCCCAGA GTGTCCTGTC ATGCTTGATT CCAGGAACTG TATAACTCTG 
GTGGTTCCAT TTGCAGAGAC TATTCGTAAA ATGACCAATC AATCTTCAAA AGTCCTGAAG 
CAGTGTTGGA CATCTTTGCA AGAATCTTCT CTCAACTCAC TTGTCCAGAT GCTGAAAACT 
GCCATCATTT CTCAAATGAT TTGCTGGACT GAAACTGTTC AGAGTAACCA CAATGTTAAA 
ACTCTTCTGG AAGTGATGAA AGACGTGTAT AAGGTAAACA AAACTAACTG TCGATTACCA 
GAAGATATGT TCCACATAAA TGAATTCTCC TTTTTGTTGG ACTTTTATGA AGATAGAAGA 
AGAGTGATCA ATAGGGAAAA AAACCTGATA CCTGCAGAAA ACTTCAATCT GATTATTTTC 
AGTGATTTTC CATTTGCCTT TAATTTGGTG TCCAAAATTA AATTATTGCA AGCTGATTCC 
CAAATAAGAA TGCTGAAATC TGAAGTGAAC AACTACATGG ATTTTAGGGG AATAATTCTG 
CCAAGAAGGG ATGAATCTCC TTCATTTACG CTTAGAGTCA GACGAAGTCA CTTAGTTGAA 
GATGCTCTAT GTCAGTTAAG CCAAGCTGAA GACACTGACC TCCAGAAAAT ATTAGTGGTT 
GAATTTATTA AAGAAATTCG TTCTGTGGGG GAAGGGGTGA AGTCTGAGTT TTTTCACTGT 
ATATTTGAAT CTATGACCAA GGAAGAATAT GGAATGTTCA TATATCCCGA GGAGGATTCC 
TACATGTGGT TTCCTGTCAA ACCTAAACTT GAGAAGAAGA TGTATTTCCT CTTTGGGATG 
CTGTGTGGAC TCTCCCTATA TAATTTTAAT GTTGTCTACC TCCCTTTCCC ACTGGCTCTG 
TTTAAGAAAC TTCTGAACCA GGAGCCATCA TTGGAAGATT TAAAAGAACT CAGTCCTTCT 
TTTGGAAAGT GTTTGCAAGA AGTTCTAAAT GATGATGCTA ATGACATTAA AGAAGAATTT 
GGCCTACGTT TTTCTATACA CTGGGACCAA AATGATGTTG ATTTAATTCC AAATGGGATT 
TCTGTATTTG TAGACCAAAG CAACAAGAAA GACTATGTTT CTAAGTGTGT TGATTATGTT 
TTCAACACCT CTGTAAAGGC AGTTTATGAG GAATTTCAGA GAGGATTTTA TAAACTTTTT 
GACAAAGAGA TACTTAAACA TTTCAAGCCT GAAGAACTAA TGAGAGCAAT AATTGGAAAT 
ACTGACTATG ACTGGGAACA ATTTGAAAAG AATTCTATAT ATGAGCAAGG ATACCATGAG 
TCACATCCTA CTATACTGAT GTTTTGGAAA GCTTTCCATA ATTTAACTTT GGATGAAAAG 
AGAAAATTCC TCTTTTTTCT TACAGGAAAT GATAGACTAC ATGTAAAAGG CATACAGAAA 
CCAGGAATCC GGTTTTGCTG TCCTGAGACT TGCAGTGAAA GCGATTTCCC AAAATCACTG 
ACATGTCATA ATATTCTGGA ACTCCCTAAA TATTCTACAA TGGAAAAAGT GAAGGAAGCA 
CTTCAAATAG TCATCAACAG CAACAAAGGA TTTATCTCAG CCACAGTCAC AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006043312.1
CDS	105..3161
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQKDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_006043250.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006043250.2

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGTGAAAT TAGAGTGATA TTTTCATCTC CTGCTTGTCT GACTGCAAGT 
TTTTTAAAGA AGAGAGAACC TGGAGAAATT ATTTCCATTG ATGTGGACTT AGAAATGGCA 
AGAAATACCT TCAAGAAGTT AACAGAAAAA GAATGGATTT CTTCGATGAT AACCACATGT 
CTCAAGGATA ATCTGCTTGG GGCTCTTCCA TGTCGTTCTT CACACCAAGA AGCTTTATCA 
GTCTTCCTTC TGCTCCCAGA GTGTCCTGTC ATGCTTGATT CCAGGAACTG TATAACTCTG 
GTGGTTCCAT TTGCAGAGAC TATTCGTAAA ATGACCAATC AATCTTCAAA AGTCCTGAAG 
CAGTGTTGGA CATCTTTGCA AGAATCTTCT CTCAACTCAC TTGTCCAGAT GCTGAAAACT 
GCCATCATTT CTCAAATGAT TTGCTGGACT GAAACTGTTC AGAGTAACCA CAATGTTAAA 
ACTCTTCTGG AAGTGATGAA AGACGTGTAT AAGGTAAACA AAACTAACTG TCGATTACCA 
GAAGATATGT TCCACATAAA TGAATTCTCC TTTTTGTTGG ACTTTTATGA AGATAGAAGA 
AGAGTGATCA ATAGGGAAAA AAACCTGATA CCTGCAGAAA ACTTCAATCT GATTATTTTC 
AGTGATTTTC CATTTGCCTT TAATTTGGTG TCCAAAATTA AATTATTGCA AGCTGATTCC 
CAAATAAGAA TGCTGAAATC TGAAGTGAAC AACTACATGG ATTTTAGGGG AATAATTCTG 
CCAAGAAGGG ATGAATCTCC TTCATTTACG CTTAGAGTCA GACGAAGTCA CTTAGTTGAA 
GATGCTCTAT GTCAGTTAAG CCAAGCTGAA GACACTGACC TCCAGAAAAT ATTAGTGGTT 
GAATTTATTA AAGAAATTCG TTCTGTGGGG GAAGGGGTGA AGTCTGAGTT TTTTCACTGT 
ATATTTGAAT CTATGACCAA GGAAGAATAT GGAATGTTCA TATATCCCGA GGAGGATTCC 
TACATGTGGT TTCCTGTCAA ACCTAAACTT GAGAAGAAGA TGTATTTCCT CTTTGGGATG 
CTGTGTGGAC TCTCCCTATA TAATTTTAAT GTTGTCTACC TCCCTTTCCC ACTGGCTCTG 
TTTAAGAAAC TTCTGAACCA GGAGCCATCA TTGGAAGATT TAAAAGAACT CAGTCCTTCT 
TTTGGAAAGT GTTTGCAAGA AGTTCTAAAT GATGATGCTA ATGACATTAA AGAAGAATTT 
GGCCTACGTT TTTCTATACA CTGGGACCAA AATGATGTTG ATTTAATTCC AAATGGGATT 
TCTGTATTTG TAGACCAAAG CAACAAGAAA GACTATGTTT CTAAGTGTGT TGATTATGTT 
TTCAACACCT CTGTAAAGGC AGTTTATGAG GAATTTCAGA GAGGATTTTA TAAACTTTTT 
GACAAAGAGA TACTTAAACA TTTCAAGCCT GAAGAACTAA TGAGAGCAAT AATTGGAAAT 
ACTGACTATG ACTGGGAACA ATTTGAAAAG AATTCTATAT ATGAGCAAGG ATACCATGAG 
TCACATCCTA CTATACTGAT GTTTTGGAAA GCTTTCCATA ATTTAACTTT GGATGAAAAG 
AGAAAATTCC TCTTTTTTCT TACAGGAAAT GATAGACTAC ATGTAAAAGG CATACAGAAA 
CCAGGAATCC GGTTTTGCTG TCCTGAGACT TGCAGTGAAA GCGATTTCCC AAAATCACTG 
ACATGTCATA ATATTCTGGA ACTCCCTAAA TATTCTACAA TGGAAAAAGT GAAGGAAGCA 
CTTCAAATAG TCATCAACAG CAACAAAGGA TTTATCTCAG CCACAGTCAC AGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_006043251.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006043313.1 Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu02634
Clone ID Related Accession (Same CDS sequence)	XM_006043251.1 , XM_006043251.2
Accession Version	XM_006043251.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783330.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAATCTGAT TATTTTCAGT 
GATTTTCCAT TTGCCTTTAA TTTGGTGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAATGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCCA 
AGAAGGGATG AATCTCCTTC ATTTACGCTT AGAGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGTGGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATAT ATCCCGAGGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAACTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGAACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGTT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTC 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATATATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAATT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTTGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTAAATAT TCTACAATGG AAAAAGTGAA GGAAGCACTT 
CAAATAGTCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006043313.1
CDS	347..3400
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_006043251.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 (HERC6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006043251.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAATCTGAT TATTTTCAGT 
GATTTTCCAT TTGCCTTTAA TTTGGTGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAATGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCCA 
AGAAGGGATG AATCTCCTTC ATTTACGCTT AGAGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGTGGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATAT ATCCCGAGGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAACTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGAACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGTT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTC 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATATATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAATT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTTGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTAAATAT TCTACAATGG AAAAAGTGAA GGAAGCACTT 
CAAATAGTCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_006043251.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006043313.1 Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu02634
Clone ID Related Accession (Same CDS sequence)	XM_006043251.1 , XM_006043251.2
Accession Version	XM_006043251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAATCTGAT TATTTTCAGT 
GATTTTCCAT TTGCCTTTAA TTTGGTGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAATGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCCA 
AGAAGGGATG AATCTCCTTC ATTTACGCTT AGAGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGTGGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATAT ATCCCGAGGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAACTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGAACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGTT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTC 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATATATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAATT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTTGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTAAATAT TCTACAATGG AAAAAGTGAA GGAAGCACTT 
CAAATAGTCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006043313.1
CDS	105..3158
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKSEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_006043251.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006043251.2

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GTGAAATTAG AGTGATATTT TCATCTCCTG CTTGTCTGAC TGCAAGTTTT 
TTAAAGAAGA GAGAACCTGG AGAAATTATT TCCATTGATG TGGACTTAGA AATGGCAAGA 
AATACCTTCA AGAAGTTAAC AGAAAAAGAA TGGATTTCTT CGATGATAAC CACATGTCTC 
AAGGATAATC TGCTTGGGGC TCTTCCATGT CGTTCTTCAC ACCAAGAAGC TTTATCAGTC 
TTCCTTCTGC TCCCAGAGTG TCCTGTCATG CTTGATTCCA GGAACTGTAT AACTCTGGTG 
GTTCCATTTG CAGAGACTAT TCGTAAAATG ACCAATCAAT CTTCAAAAGT CCTGAAGCAG 
TGTTGGACAT CTTTGCAAGA ATCTTCTCTC AACTCACTTG TCCAGATGCT GAAAACTGCC 
ATCATTTCTC AAATGATTTG CTGGACTGAA ACTGTTCAGA GTAACCACAA TGTTAAAACT 
CTTCTGGAAG TGATGAAAGA CGTGTATAAG GTAAACAAAA CTAACTGTCG ATTACCAGAA 
GATATGTTCC ACATAAATGA ATTCTCCTTT TTGTTGGACT TTTATGAAGA TAGAAGAAGA 
GTGATCAATA GGGAAAAAAA CCTGATACCT GCAGAAAACT TCAATCTGAT TATTTTCAGT 
GATTTTCCAT TTGCCTTTAA TTTGGTGTCC AAAATTAAAT TATTGCAAGC TGATTCCCAA 
ATAAGAATGC TGAAATCTGA AGTGAACAAC TACATGGATT TTAGGGGAAT AATTCTGCCA 
AGAAGGGATG AATCTCCTTC ATTTACGCTT AGAGTCAGAC GAAGTCACTT AGTTGAAGAT 
GCTCTATGTC AGTTAAGCCA AGCTGAAGAC ACTGACCTCC AGAAAATATT AGTGGTTGAA 
TTTATTAAAG AAATTCGTTC TGTGGGGGAA GGGGTGAAGT CTGAGTTTTT TCACTGTATA 
TTTGAATCTA TGACCAAGGA AGAATATGGA ATGTTCATAT ATCCCGAGGA GGATTCCTAC 
ATGTGGTTTC CTGTCAAACC TAAACTTGAG AAGAAGATGT ATTTCCTCTT TGGGATGCTG 
TGTGGACTCT CCCTATATAA TTTTAATGTT GTCTACCTCC CTTTCCCACT GGCTCTGTTT 
AAGAAACTTC TGAACCAGGA GCCATCATTG GAAGATTTAA AAGAACTCAG TCCTTCTTTT 
GGAAAGTGTT TGCAAGAAGT TCTAAATGAT GATGCTAATG ACATTAAAGA AGAATTTGGC 
CTACGTTTTT CTATACACTG GGACCAAAAT GATGTTGATT TAATTCCAAA TGGGATTTCT 
GTATTTGTAG ACCAAAGCAA CAAGAAAGAC TATGTTTCTA AGTGTGTTGA TTATGTTTTC 
AACACCTCTG TAAAGGCAGT TTATGAGGAA TTTCAGAGAG GATTTTATAA ACTTTTTGAC 
AAAGAGATAC TTAAACATTT CAAGCCTGAA GAACTAATGA GAGCAATAAT TGGAAATACT 
GACTATGACT GGGAACAATT TGAAAAGAAT TCTATATATG AGCAAGGATA CCATGAGTCA 
CATCCTACTA TACTGATGTT TTGGAAAGCT TTCCATAATT TAACTTTGGA TGAAAAGAGA 
AAATTCCTCT TTTTTCTTAC AGGAAATGAT AGACTACATG TAAAAGGCAT ACAGAAACCA 
GGAATCCGGT TTTGCTGTCC TGAGACTTGC AGTGAAAGCG ATTTCCCAAA ATCACTGACA 
TGTCATAATA TTCTGGAACT CCCTAAATAT TCTACAATGG AAAAAGTGAA GGAAGCACTT 
CAAATAGTCA TCAACAGCAA CAAAGGATTT ATCTCAGCCA CAGTCACAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_025290428.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025146213.1 Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu70196
Clone ID Related Accession (Same CDS sequence)	XM_025290428.1
Accession Version	XM_025290428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGGTGGCT CTATCTGATA AATGAAATTA GAGTGATATT TTCATCTCCT 
GCTTGTCTGA CTGCAAGTTT TTTAAAGAAG AGAGAACCTG GAGAAATTAT TTCCATTGAT 
GTGGACTTAG AAATGGCAAG AAATACCTTC AAGAAGTTAA CAGAAAAAGA ATGGATTTCT 
TCGATGATAA CCACATGTCT CAAGGATAAT CTGCTTGGGG CTCTTCCATG TCGTTCTTCA 
CACCAAGAAG CTTTATCAGT CTTCCTTCTG CTCCCAGAGT GTCCTGTCAT GCTTGATTCC 
AGGAACTGTA TAACTCTGGT GGTTCCATTT GCAGAGACTA TTCGTAAAAT GACCAATCAA 
TCTTCAAAAG TCCTGAAGCA GTGTTGGACA TCTTTGCAAG AATCTTCTCT CAACTCACTT 
GTCCAGATGC TGAAAACTGC CATCATTTCT CAAATGATTT GCTGGACTGA AACTGTTCAG 
AGTAACCACA ATGTTAAAAC TCTTCTGGAA GTGATGAAAG ACGTGTATAA GGTAAACAAA 
ACTAACTGTC GATTACCAGA AGATATGTTC CACATAAATG AATTCTCCTT TTTGTTGGAC 
TTTTATGAAG ATAGAAGAAG AGTGATCAAT AGGGAAAAAA ACCTGATACC TGCAGAAAAC 
TTCAATCTGA TTATTTTCAG TGATTTTCCA TTTGCCTTTA ATTTGGTGTC CAAAATTAAA 
TTATTGCAAG CTGATTCCCA AATAAGAATG CTGAAATCTG AAGTGAACAA CTACATGGAT 
TTTAGGGGAA TAATTCTGCC AAGAAGGGAT GAATCTCCTT CATTTACGCT TAGAGTCAGA 
CGAAGTCACT TAGTTGAAGA TGCTCTATGT CAGTTAAGCC AAGCTGAAGA CACTGACCTC 
CAGAAAATAT TAGTGGTTGA ATTTATTAAA GAAATTCGTT CTGTGGGGGA AGGGGTGAAG 
TCTGAGTTTT TTCACTGTAT ATTTGAATCT ATGACCAAGG AAGAATATGG AATGTTCATA 
TATCCCGAGG AGGATTCCTA CATGTGGTTT CCTGTCAAAC CTAAACTTGA GAAGAAGATG 
TATTTCCTCT TTGGGATGCT GTGTGGACTC TCCCTATATA ATTTTAATGT TGTCTACCTC 
CCTTTCCCAC TGGCTCTGTT TAAGAAACTT CTGAACCAGG AGCCATCATT GGAAGATTTA 
AAAGAACTCA GTCCTTCTTT TGGAAAGTGT TTGCAAGAAG TTCTAAATGA TGATGCTAAT 
GACATTAAAG AAGAATTTGG CCTACGTTTT TCTATACACT GGGACCAAAA TGATGTTGAT 
TTAATTCCAA ATGGGATTTC TGTATTTGTA GACCAAAGCA ACAAGAAAGA CTATGTTTCT 
AAGTGTGTTG ATTATGTTTT CAACACCTCT GTAAAGGCAG TTTATGAGGA ATTTCAGAGA 
GGATTTTATA AACTTTTTGA CAAAGAGATA CTTAAACATT TCAAGCCTGA AGAACTAATG 
AGAGCAATAA TTGGAAATAC TGACTATGAC TGGGAACAAT TTGAAAAGAA TTCTATATAT 
GAGCAAGGAT ACCATGAGTC ACATCCTACT ATACTGATGT TTTGGAAAGC TTTCCATAAT 
TTAACTTTGG ATGAAAAGAG AAAATTCCTC TTTTTTCTTA CAGGAAATGA TAGACTACAT 
GTAAAAGGCA TACAGAAACC AGGAATCCGG TTTTGCTGTC CTGAGACTTG CAGTGAAAGC 
GATTTCCCAA AATCACTGAC ATGTCATAAT ATTCTGGAAC TCCCTAAATA TTCTACAATG 
GAAAAAGTGA AGGAAGCACT TCAAATAGTC ATCAACAGCA ACAAAGGATT TATCTCAGCC 
ACAGTCACAG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025146213.1
CDS	105..3179
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQKDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKRWLYLINEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_025290428.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025290428.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGA AGGATACTAG TTCCACCAGT GTTTCCAGGA AAACCCTGCC AGAAATAAGC 
CGAATTAACC AGTCTCTGAT AGAAAAATGG ATGGCAGTGG CAAAAAGAAG TATAGAAGAT 
GAAGTGGCTA AGAGGTGGCT CTATCTGATA AATGAAATTA GAGTGATATT TTCATCTCCT 
GCTTGTCTGA CTGCAAGTTT TTTAAAGAAG AGAGAACCTG GAGAAATTAT TTCCATTGAT 
GTGGACTTAG AAATGGCAAG AAATACCTTC AAGAAGTTAA CAGAAAAAGA ATGGATTTCT 
TCGATGATAA CCACATGTCT CAAGGATAAT CTGCTTGGGG CTCTTCCATG TCGTTCTTCA 
CACCAAGAAG CTTTATCAGT CTTCCTTCTG CTCCCAGAGT GTCCTGTCAT GCTTGATTCC 
AGGAACTGTA TAACTCTGGT GGTTCCATTT GCAGAGACTA TTCGTAAAAT GACCAATCAA 
TCTTCAAAAG TCCTGAAGCA GTGTTGGACA TCTTTGCAAG AATCTTCTCT CAACTCACTT 
GTCCAGATGC TGAAAACTGC CATCATTTCT CAAATGATTT GCTGGACTGA AACTGTTCAG 
AGTAACCACA ATGTTAAAAC TCTTCTGGAA GTGATGAAAG ACGTGTATAA GGTAAACAAA 
ACTAACTGTC GATTACCAGA AGATATGTTC CACATAAATG AATTCTCCTT TTTGTTGGAC 
TTTTATGAAG ATAGAAGAAG AGTGATCAAT AGGGAAAAAA ACCTGATACC TGCAGAAAAC 
TTCAATCTGA TTATTTTCAG TGATTTTCCA TTTGCCTTTA ATTTGGTGTC CAAAATTAAA 
TTATTGCAAG CTGATTCCCA AATAAGAATG CTGAAATCTG AAGTGAACAA CTACATGGAT 
TTTAGGGGAA TAATTCTGCC AAGAAGGGAT GAATCTCCTT CATTTACGCT TAGAGTCAGA 
CGAAGTCACT TAGTTGAAGA TGCTCTATGT CAGTTAAGCC AAGCTGAAGA CACTGACCTC 
CAGAAAATAT TAGTGGTTGA ATTTATTAAA GAAATTCGTT CTGTGGGGGA AGGGGTGAAG 
TCTGAGTTTT TTCACTGTAT ATTTGAATCT ATGACCAAGG AAGAATATGG AATGTTCATA 
TATCCCGAGG AGGATTCCTA CATGTGGTTT CCTGTCAAAC CTAAACTTGA GAAGAAGATG 
TATTTCCTCT TTGGGATGCT GTGTGGACTC TCCCTATATA ATTTTAATGT TGTCTACCTC 
CCTTTCCCAC TGGCTCTGTT TAAGAAACTT CTGAACCAGG AGCCATCATT GGAAGATTTA 
AAAGAACTCA GTCCTTCTTT TGGAAAGTGT TTGCAAGAAG TTCTAAATGA TGATGCTAAT 
GACATTAAAG AAGAATTTGG CCTACGTTTT TCTATACACT GGGACCAAAA TGATGTTGAT 
TTAATTCCAA ATGGGATTTC TGTATTTGTA GACCAAAGCA ACAAGAAAGA CTATGTTTCT 
AAGTGTGTTG ATTATGTTTT CAACACCTCT GTAAAGGCAG TTTATGAGGA ATTTCAGAGA 
GGATTTTATA AACTTTTTGA CAAAGAGATA CTTAAACATT TCAAGCCTGA AGAACTAATG 
AGAGCAATAA TTGGAAATAC TGACTATGAC TGGGAACAAT TTGAAAAGAA TTCTATATAT 
GAGCAAGGAT ACCATGAGTC ACATCCTACT ATACTGATGT TTTGGAAAGC TTTCCATAAT 
TTAACTTTGG ATGAAAAGAG AAAATTCCTC TTTTTTCTTA CAGGAAATGA TAGACTACAT 
GTAAAAGGCA TACAGAAACC AGGAATCCGG TTTTGCTGTC CTGAGACTTG CAGTGAAAGC 
GATTTCCCAA AATCACTGAC ATGTCATAAT ATTCTGGAAC TCCCTAAATA TTCTACAATG 
GAAAAAGTGA AGGAAGCACT TCAAATAGTC ATCAACAGCA ACAAAGGATT TATCTCAGCC 
ACAGTCACAG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102415279 ( XM_025290429.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025146214.1 Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X2, mRNA.

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CloneID	OBu70197
Clone ID Related Accession (Same CDS sequence)	XM_025290429.1
Accession Version	XM_025290429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GGTGGCTCTA TCTGATAAAT GAAATTAGAG TGATATTTTC ATCTCCTGCT 
TGTCTGACTG CAAGTTTTTT AAAGAAGAGA GAACCTGGAG AAATTATTTC CATTGATGTG 
GACTTAGAAA TGGCAAGAAA TACCTTCAAG AAGTTAACAG AAAAAGAATG GATTTCTTCG 
ATGATAACCA CATGTCTCAA GGATAATCTG CTTGGGGCTC TTCCATGTCG TTCTTCACAC 
CAAGAAGCTT TATCAGTCTT CCTTCTGCTC CCAGAGTGTC CTGTCATGCT TGATTCCAGG 
AACTGTATAA CTCTGGTGGT TCCATTTGCA GAGACTATTC GTAAAATGAC CAATCAATCT 
TCAAAAGTCC TGAAGCAGTG TTGGACATCT TTGCAAGAAT CTTCTCTCAA CTCACTTGTC 
CAGATGCTGA AAACTGCCAT CATTTCTCAA ATGATTTGCT GGACTGAAAC TGTTCAGAGT 
AACCACAATG TTAAAACTCT TCTGGAAGTG ATGAAAGACG TGTATAAGGT AAACAAAACT 
AACTGTCGAT TACCAGAAGA TATGTTCCAC ATAAATGAAT TCTCCTTTTT GTTGGACTTT 
TATGAAGATA GAAGAAGAGT GATCAATAGG GAAAAAAACC TGATACCTGC AGAAAACTTC 
AATCTGATTA TTTTCAGTGA TTTTCCATTT GCCTTTAATT TGGTGTCCAA AATTAAATTA 
TTGCAAGCTG ATTCCCAAAT AAGAATGCTG AAATCTGAAG TGAACAACTA CATGGATTTT 
AGGGGAATAA TTCTGCCAAG AAGGGATGAA TCTCCTTCAT TTACGCTTAG AGTCAGACGA 
AGTCACTTAG TTGAAGATGC TCTATGTCAG TTAAGCCAAG CTGAAGACAC TGACCTCCAG 
AAAATATTAG TGGTTGAATT TATTAAAGAA ATTCGTTCTG TGGGGGAAGG GGTGAAGTCT 
GAGTTTTTTC ACTGTATATT TGAATCTATG ACCAAGGAAG AATATGGAAT GTTCATATAT 
CCCGAGGAGG ATTCCTACAT GTGGTTTCCT GTCAAACCTA AACTTGAGAA GAAGATGTAT 
TTCCTCTTTG GGATGCTGTG TGGACTCTCC CTATATAATT TTAATGTTGT CTACCTCCCT 
TTCCCACTGG CTCTGTTTAA GAAACTTCTG AACCAGGAGC CATCATTGGA AGATTTAAAA 
GAACTCAGTC CTTCTTTTGG AAAGTGTTTG CAAGAAGTTC TAAATGATGA TGCTAATGAC 
ATTAAAGAAG AATTTGGCCT ACGTTTTTCT ATACACTGGG ACCAAAATGA TGTTGATTTA 
ATTCCAAATG GGATTTCTGT ATTTGTAGAC CAAAGCAACA AGAAAGACTA TGTTTCTAAG 
TGTGTTGATT ATGTTTTCAA CACCTCTGTA AAGGCAGTTT ATGAGGAATT TCAGAGAGGA 
TTTTATAAAC TTTTTGACAA AGAGATACTT AAACATTTCA AGCCTGAAGA ACTAATGAGA 
GCAATAATTG GAAATACTGA CTATGACTGG GAACAATTTG AAAAGAATTC TATATATGAG 
CAAGGATACC ATGAGTCACA TCCTACTATA CTGATGTTTT GGAAAGCTTT CCATAATTTA 
ACTTTGGATG AAAAGAGAAA ATTCCTCTTT TTTCTTACAG GAAATGATAG ACTACATGTA 
AAAGGCATAC AGAAACCAGG AATCCGGTTT TGCTGTCCTG AGACTTGCAG TGAAAGCGAT 
TTCCCAAAAT CACTGACATG TCATAATATT CTGGAACTCC CTAAATATTC TACAATGGAA 
AAAGTGAAGG AAGCACTTCA AATAGTCATC AACAGCAACA AAGGATTTAT CTCAGCCACA 
GTCACAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025146214.1
CDS	107..3178
Translation	MYFCWRAGRRRPLRERQVCAGGNTLLQAASGEHHSLLLLSDGTVQSCGDNSRGQLGRKGTPRGEQPERIPALETLHVALVSCGKEHSLAVCHKGRVFAWGAASEGQLGIGGFKETCIPKKIKTLAGIKIIQVACGHYHSVALSEDGQVFSWGKNSHGQLGLGKEFPSQAIPQRVRSLEGIPLAQVAAGGDHSFALSLSGTSFGWGSNNAGQLALSGNNAPVQRYKPVSVGALKTLGVVSISCGSKHTAVLTQDGKVFTFGDNSYGQLGHDPTAEKRGPQLVERIEGLVSQIDCGSLHTLAYVYTTGQVVSFGRGPGCASSSPHPGALAESSDVSCLISANDLADVQVKHIFAGTYANFVTTYQDTSSTSVSRKTLPEISRINQSLIEKWMAVAKRSIEDEVAKRWLYLINEIRVIFSSPACLTASFLKKREPGEIISIDVDLEMARNTFKKLTEKEWISSMITTCLKDNLLGALPCRSSHQEALSVFLLLPECPVMLDSRNCITLVVPFAETIRKMTNQSSKVLKQCWTSLQESSLNSLVQMLKTAIISQMICWTETVQSNHNVKTLLEVMKDVYKVNKTNCRLPEDMFHINEFSFLLDFYEDRRRVINREKNLIPAENFNLIIFSDFPFAFNLVSKIKLLQADSQIRMLKSEVNNYMDFRGIILPRRDESPSFTLRVRRSHLVEDALCQLSQAEDTDLQKILVVEFIKEIRSVGEGVKSEFFHCIFESMTKEEYGMFIYPEEDSYMWFPVKPKLEKKMYFLFGMLCGLSLYNFNVVYLPFPLALFKKLLNQEPSLEDLKELSPSFGKCLQEVLNDDANDIKEEFGLRFSIHWDQNDVDLIPNGISVFVDQSNKKDYVSKCVDYVFNTSVKAVYEEFQRGFYKLFDKEILKHFKPEELMRAIIGNTDYDWEQFEKNSIYEQGYHESHPTILMFWKAFHNLTLDEKRKFLFFLTGNDRLHVKGIQKPGIRFCCPETCSESDFPKSLTCHNILELPKYSTMEKVKEALQIVINSNKGFISATVTG

Target ORF information:

RefSeq Version	XM_025290429.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis probable E3 ubiquitin-protein ligase HERC6 (LOC102415279), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025290429.1

ORF Insert Sequence:

ATGTACTTCT GCTGGCGCGC TGGCCGCCGA AGGCCGCTGC GGGAGCGCCA GGTGTGTGCT 
GGCGGCAACA CGCTACTGCA GGCGGCCAGT GGGGAGCATC ACTCGCTACT GCTGCTGAGC 
GACGGCACGG TCCAGTCGTG CGGGGACAAC AGCCGGGGCC AGCTGGGCCG GAAAGGCACG 
CCGCGCGGGG AGCAGCCAGA ACGAATTCCG GCATTGGAAA CTCTGCACGT GGCTCTGGTG 
AGCTGTGGAA AGGAGCACTC CCTGGCTGTC TGTCACAAAG GAAGGGTCTT TGCGTGGGGA 
GCTGCTTCTG AAGGACAGCT GGGAATTGGA GGATTTAAGG AAACGTGTAT ACCTAAGAAG 
ATAAAAACTC TGGCTGGTAT AAAAATTATA CAAGTTGCCT GTGGACACTA CCACTCCGTG 
GCATTATCAG AAGATGGCCA AGTGTTTTCA TGGGGGAAGA ACAGCCATGG ACAGCTGGGC 
CTGGGGAAGG AGTTCCCCTC CCAAGCTATC CCACAGAGGG TGAGGTCCCT GGAGGGGATC 
CCGCTGGCAC AGGTGGCTGC AGGAGGGGAC CACAGCTTTG CCCTGTCACT CTCTGGGACT 
TCATTTGGCT GGGGAAGCAA CAATGCAGGG CAGCTGGCCC TCAGTGGGAA TAACGCCCCA 
GTTCAAAGAT ATAAGCCTGT ATCAGTTGGT GCACTGAAGA CTCTCGGTGT GGTTTCTATC 
AGCTGTGGTT CCAAGCACAC TGCAGTGCTT ACCCAGGATG GGAAAGTATT CACATTTGGA 
GACAATAGCT ATGGACAGCT GGGACACGAT CCCACTGCTG AGAAGAGGGG TCCACAACTT 
GTGGAAAGAA TTGAAGGCCT CGTTTCACAG ATAGACTGTG GAAGTCTTCA CACCCTTGCA 
TATGTCTATA CCACTGGTCA GGTGGTGTCT TTCGGTCGTG GACCAGGTTG TGCAAGCAGC 
TCACCACATC CGGGGGCTCT GGCAGAGAGC TCTGACGTTA GCTGCCTGAT TTCTGCTAAT 
GACCTTGCGG ATGTTCAAGT CAAACATATT TTTGCTGGAA CATATGCCAA CTTTGTGACA 
ACTTACCAGG ATACTAGTTC CACCAGTGTT TCCAGGAAAA CCCTGCCAGA AATAAGCCGA 
ATTAACCAGT CTCTGATAGA AAAATGGATG GCAGTGGCAA AAAGAAGTAT AGAAGATGAA 
GTGGCTAAGA GGTGGCTCTA TCTGATAAAT GAAATTAGAG TGATATTTTC ATCTCCTGCT 
TGTCTGACTG CAAGTTTTTT AAAGAAGAGA GAACCTGGAG AAATTATTTC CATTGATGTG 
GACTTAGAAA TGGCAAGAAA TACCTTCAAG AAGTTAACAG AAAAAGAATG GATTTCTTCG 
ATGATAACCA CATGTCTCAA GGATAATCTG CTTGGGGCTC TTCCATGTCG TTCTTCACAC 
CAAGAAGCTT TATCAGTCTT CCTTCTGCTC CCAGAGTGTC CTGTCATGCT TGATTCCAGG 
AACTGTATAA CTCTGGTGGT TCCATTTGCA GAGACTATTC GTAAAATGAC CAATCAATCT 
TCAAAAGTCC TGAAGCAGTG TTGGACATCT TTGCAAGAAT CTTCTCTCAA CTCACTTGTC 
CAGATGCTGA AAACTGCCAT CATTTCTCAA ATGATTTGCT GGACTGAAAC TGTTCAGAGT 
AACCACAATG TTAAAACTCT TCTGGAAGTG ATGAAAGACG TGTATAAGGT AAACAAAACT 
AACTGTCGAT TACCAGAAGA TATGTTCCAC ATAAATGAAT TCTCCTTTTT GTTGGACTTT 
TATGAAGATA GAAGAAGAGT GATCAATAGG GAAAAAAACC TGATACCTGC AGAAAACTTC 
AATCTGATTA TTTTCAGTGA TTTTCCATTT GCCTTTAATT TGGTGTCCAA AATTAAATTA 
TTGCAAGCTG ATTCCCAAAT AAGAATGCTG AAATCTGAAG TGAACAACTA CATGGATTTT 
AGGGGAATAA TTCTGCCAAG AAGGGATGAA TCTCCTTCAT TTACGCTTAG AGTCAGACGA 
AGTCACTTAG TTGAAGATGC TCTATGTCAG TTAAGCCAAG CTGAAGACAC TGACCTCCAG 
AAAATATTAG TGGTTGAATT TATTAAAGAA ATTCGTTCTG TGGGGGAAGG GGTGAAGTCT 
GAGTTTTTTC ACTGTATATT TGAATCTATG ACCAAGGAAG AATATGGAAT GTTCATATAT 
CCCGAGGAGG ATTCCTACAT GTGGTTTCCT GTCAAACCTA AACTTGAGAA GAAGATGTAT 
TTCCTCTTTG GGATGCTGTG TGGACTCTCC CTATATAATT TTAATGTTGT CTACCTCCCT 
TTCCCACTGG CTCTGTTTAA GAAACTTCTG AACCAGGAGC CATCATTGGA AGATTTAAAA 
GAACTCAGTC CTTCTTTTGG AAAGTGTTTG CAAGAAGTTC TAAATGATGA TGCTAATGAC 
ATTAAAGAAG AATTTGGCCT ACGTTTTTCT ATACACTGGG ACCAAAATGA TGTTGATTTA 
ATTCCAAATG GGATTTCTGT ATTTGTAGAC CAAAGCAACA AGAAAGACTA TGTTTCTAAG 
TGTGTTGATT ATGTTTTCAA CACCTCTGTA AAGGCAGTTT ATGAGGAATT TCAGAGAGGA 
TTTTATAAAC TTTTTGACAA AGAGATACTT AAACATTTCA AGCCTGAAGA ACTAATGAGA 
GCAATAATTG GAAATACTGA CTATGACTGG GAACAATTTG AAAAGAATTC TATATATGAG 
CAAGGATACC ATGAGTCACA TCCTACTATA CTGATGTTTT GGAAAGCTTT CCATAATTTA 
ACTTTGGATG AAAAGAGAAA ATTCCTCTTT TTTCTTACAG GAAATGATAG ACTACATGTA 
AAAGGCATAC AGAAACCAGG AATCCGGTTT TGCTGTCCTG AGACTTGCAG TGAAAGCGAT 
TTCCCAAAAT CACTGACATG TCATAATATT CTGGAACTCC CTAAATATTC TACAATGGAA 
AAAGTGAAGG AAGCACTTCA AATAGTCATC AACAGCAACA AAGGATTTAT CTCAGCCACA 
GTCACAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC102415279 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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