SMG5 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG5
Entrez Gene ID	102414866
Full Name	SMG5, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025287675.1	XP_025143460.1	protein SMG5 isoform X5
XM_006051986.2	XP_006052048.2	protein SMG5 isoform X1
XM_025287676.1	XP_025143461.1	protein SMG5 isoform X6
XM_025287672.1	XP_025143457.1	protein SMG5 isoform X2
XM_025287674.1	XP_025143459.1	protein SMG5 isoform X4
XM_006051986.1	XP_006052048.1	protein SMG5
XM_025287673.1	XP_025143458.1	protein SMG5 isoform X3

Pathways from BioSystems
Gene Ontology

The following SMG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu67925	XM_025287672.1 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu67926	XM_025287673.1 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu67927	XM_025287674.1 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu67928	XM_025287675.1 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu67929	XM_025287676.1 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu09776	XM_006051986.1 Latest version!	Bubalus bubalis SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu67924	XM_006051986.2 Latest version!	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG5 ( XM_025287672.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143457.1 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu67925
Clone ID Related Accession (Same CDS sequence)	XM_025287672.1
Accession Version	XM_025287672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGAAGCACT 
GACTTGAAAA GCATCCGCTG CCTCTCCCCA CCCAGCTCCT CCCCAGGGAG GAACCAAAGA 
AGTGATGTTA TCAGGAGGAG AAACAAGCTG GGCTCAGCAG CTGGGGAGCC ACTTAGAATG 
GCTCCAGACG GACCTGTGAC GTGGGAGACA CCCCTTCCCC TCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143457.1
CDS	161..3088
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRSTDLKSIRCLSPPSSSPGRNQRSDVIRRRNKLGSAAGEPLRMAPDGPVTWETPLPLP

Target ORF information:

RefSeq Version	XM_025287672.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287672.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGAAGCACT 
GACTTGAAAA GCATCCGCTG CCTCTCCCCA CCCAGCTCCT CCCCAGGGAG GAACCAAAGA 
AGTGATGTTA TCAGGAGGAG AAACAAGCTG GGCTCAGCAG CTGGGGAGCC ACTTAGAATG 
GCTCCAGACG GACCTGTGAC GTGGGAGACA CCCCTTCCCC TCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_025287673.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143458.1 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu67926
Clone ID Related Accession (Same CDS sequence)	XM_025287673.1
Accession Version	XM_025287673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGC ACCCGGTGGA CTATGGGAGG AAGGCTGAGG AGCTACTGTG GAGAAAGGTA 
TACTACGAGG TTATCCAGCT TATCAAGACT AACAAAAAGC ATATCCACAG CCGGAGTACC 
TTGGAATGTG CCTACAGGAC ACACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCTCACTA CCAGCTGGAA CTGCAATGCT GCATCGACTG GACCCATGTC 
ACCGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGCCACCG ATGTCTGGTG TACCTGGGGG ATTTGTCACG GTATCAGAAT 
GAACTAGCTG GCGTAGACAC TGAGCTGCTA GCTGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAC CAGCTGGGCA CCCTTGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATTC AGTCGGAAGT GTCCTTTGAG 
GGGGCCTATG GAAACCTCAA GCGGCTGTAT GACAAGGCAG CGAAAATGTA CCACCAGCTG 
AAGAAGTGTG AGACTCGGAA ACTCTCTCCC AGCAAGAAGC GATGTAAAGA CATTAAGAGG 
TTGCTGGTGA ACTTTATGTA TCTGCAAAGC CTCCTGCAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CATCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG TCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGGGGGTG 
CACAGCTTGA AGAGAGCAGG GTCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTCTCCC ACCTCGTCAA TCACGTCAAC ATCCGGCTGC AAGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCGTT CCAGAGCGAT GGCACAGATG AACCGGAGTC CAGGGAACCC 
TTGGAGAAGG AGGAGGAGCC GGGGCCTGAG CCGCCTCCTG CAGTACCTCC AGAGGGTGAG 
GTCAGAAAGA GTCGGAAGTT CTCCCGCCTC TCCTGCCTCC GCCGCCGCCG CCACCCACCC 
AAAGCAGGTG AGGACAGTGA CCTGAGTGAA GGCTTTGACT CGGACTCAAG CCGTGACTCG 
GCCCGGGCCA GCGAGGGCTC GGATAGTGGC TCCGACAAGA GTCTTGAAGG TGGGGGAACA 
GCTTTTGACG CTGAGACAGA TTCAGAAATG AACAGTCAAG AGTCCCGATC AGACCTGGAA 
GATATGGAGG ATGAGGACGG GACACGGTCC CCAGCCCTGG AGCCATCTCG GGCCAGGTCA 
GAGGCTCCTG AATCCCTCAA TGGCCCTCTG GGCCCCAGCG AGGCCAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACCAAACGCT GCTTCCGACT GGCCCCCACC 
TTCAGCAACC TGCTCCTCCA GCCCACCACT GAAGCACCTG CCTCGGCCAC CCTCAGGCGC 
TGTGTCAATG GAGATGTGGA CAAGCCTTCA GAGCCAGCCT CTGAAGAGGG CTCGGAGTCA 
GAGGGAAGCG AGTCCAGTGG GCGCTCCTTT CGGAACGAGC GGAGCATCCA GGAGAAGCTG 
CAGGTCTTGA TGGCTGAAGG CCTGCTCCCT GCTGTGAAAG TCTTCCTGGA CTGGCTGCGG 
ACCAACCCCG ACCTCATCGT TGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGT TGAACCTGTT GCCAGCTGCT GGCGAACTCC AAGAATCTGG CCTGGTCCTG 
TGTGCTGAGG TCCAGGAGCT GCTTGAAGGC TGTGAACTGC CTGACCTCCC ATCTAGCCTG 
CTGCTCCCAG AGGACACGGC CCTTCGCAAC CTGCCTCCCC TCCGGGCAGC CCACAGACGC 
TTTAGCTTTG ATACAGCTCG GCTCTTGCTC AGCAATTTAG AGGAGACAGT CGTGCGGATC 
TGCTGCATAC GCAGCTTTGG CCACTTCGTT GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AATGCAGAGG TCGGCATCTT TGTCAGCATC GCGCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CCCAGTTCCG CATGGCACAG GAGGAAGCTC GGCGGAACAG GCTGATGAGA 
GACATGGCCC AGCTACGACT TCAGCTTGAG GTCTCTCAGC TGGAAGGGAG CCTGCAGCAG 
CCCAAGGCAC GGTCAGCAAT GTCTCCCTAC CTCGTCCCCG ACACCCAGGC CCTCTGCCAC 
CACCTCCCTG TCATCCGCCA GCTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATC 
CGATATCTGG AGGCAGAGTT TAAAAAAGGG AACAGGTACA TTCGCTGCCA GAGAGAGGCG 
GGGAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CCGATGCCTG GACGCTCTAT 
AAGATCCTGG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GAGCAGGTGA GGAGGACCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ATCCTAGCAC GCTCTCAGGC 
CCTATGCAGG CAGCCTTACA GGCCGCTGCA CGTGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAGATTGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143458.1
CDS	144..2996
Translation	MFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAARASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_025287673.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287673.1

ORF Insert Sequence:

ATGTTCCTGC ACCCGGTGGA CTATGGGAGG AAGGCTGAGG AGCTACTGTG GAGAAAGGTA 
TACTACGAGG TTATCCAGCT TATCAAGACT AACAAAAAGC ATATCCACAG CCGGAGTACC 
TTGGAATGTG CCTACAGGAC ACACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCTCACTA CCAGCTGGAA CTGCAATGCT GCATCGACTG GACCCATGTC 
ACCGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGCCACCG ATGTCTGGTG TACCTGGGGG ATTTGTCACG GTATCAGAAT 
GAACTAGCTG GCGTAGACAC TGAGCTGCTA GCTGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAC CAGCTGGGCA CCCTTGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATTC AGTCGGAAGT GTCCTTTGAG 
GGGGCCTATG GAAACCTCAA GCGGCTGTAT GACAAGGCAG CGAAAATGTA CCACCAGCTG 
AAGAAGTGTG AGACTCGGAA ACTCTCTCCC AGCAAGAAGC GATGTAAAGA CATTAAGAGG 
TTGCTGGTGA ACTTTATGTA TCTGCAAAGC CTCCTGCAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CATCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG TCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGGGGGTG 
CACAGCTTGA AGAGAGCAGG GTCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTCTCCC ACCTCGTCAA TCACGTCAAC ATCCGGCTGC AAGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCGTT CCAGAGCGAT GGCACAGATG AACCGGAGTC CAGGGAACCC 
TTGGAGAAGG AGGAGGAGCC GGGGCCTGAG CCGCCTCCTG CAGTACCTCC AGAGGGTGAG 
GTCAGAAAGA GTCGGAAGTT CTCCCGCCTC TCCTGCCTCC GCCGCCGCCG CCACCCACCC 
AAAGCAGGTG AGGACAGTGA CCTGAGTGAA GGCTTTGACT CGGACTCAAG CCGTGACTCG 
GCCCGGGCCA GCGAGGGCTC GGATAGTGGC TCCGACAAGA GTCTTGAAGG TGGGGGAACA 
GCTTTTGACG CTGAGACAGA TTCAGAAATG AACAGTCAAG AGTCCCGATC AGACCTGGAA 
GATATGGAGG ATGAGGACGG GACACGGTCC CCAGCCCTGG AGCCATCTCG GGCCAGGTCA 
GAGGCTCCTG AATCCCTCAA TGGCCCTCTG GGCCCCAGCG AGGCCAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACCAAACGCT GCTTCCGACT GGCCCCCACC 
TTCAGCAACC TGCTCCTCCA GCCCACCACT GAAGCACCTG CCTCGGCCAC CCTCAGGCGC 
TGTGTCAATG GAGATGTGGA CAAGCCTTCA GAGCCAGCCT CTGAAGAGGG CTCGGAGTCA 
GAGGGAAGCG AGTCCAGTGG GCGCTCCTTT CGGAACGAGC GGAGCATCCA GGAGAAGCTG 
CAGGTCTTGA TGGCTGAAGG CCTGCTCCCT GCTGTGAAAG TCTTCCTGGA CTGGCTGCGG 
ACCAACCCCG ACCTCATCGT TGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGT TGAACCTGTT GCCAGCTGCT GGCGAACTCC AAGAATCTGG CCTGGTCCTG 
TGTGCTGAGG TCCAGGAGCT GCTTGAAGGC TGTGAACTGC CTGACCTCCC ATCTAGCCTG 
CTGCTCCCAG AGGACACGGC CCTTCGCAAC CTGCCTCCCC TCCGGGCAGC CCACAGACGC 
TTTAGCTTTG ATACAGCTCG GCTCTTGCTC AGCAATTTAG AGGAGACAGT CGTGCGGATC 
TGCTGCATAC GCAGCTTTGG CCACTTCGTT GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AATGCAGAGG TCGGCATCTT TGTCAGCATC GCGCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CCCAGTTCCG CATGGCACAG GAGGAAGCTC GGCGGAACAG GCTGATGAGA 
GACATGGCCC AGCTACGACT TCAGCTTGAG GTCTCTCAGC TGGAAGGGAG CCTGCAGCAG 
CCCAAGGCAC GGTCAGCAAT GTCTCCCTAC CTCGTCCCCG ACACCCAGGC CCTCTGCCAC 
CACCTCCCTG TCATCCGCCA GCTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATC 
CGATATCTGG AGGCAGAGTT TAAAAAAGGG AACAGGTACA TTCGCTGCCA GAGAGAGGCG 
GGGAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CCGATGCCTG GACGCTCTAT 
AAGATCCTGG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GAGCAGGTGA GGAGGACCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ATCCTAGCAC GCTCTCAGGC 
CCTATGCAGG CAGCCTTACA GGCCGCTGCA CGTGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAGATTGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_025287674.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143459.1 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu67927
Clone ID Related Accession (Same CDS sequence)	XM_025287674.1
Accession Version	XM_025287674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGAAGCTA 
CACAAAGCCT TCTTGTTTGG ACCTTGTCTC CAGAAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143459.1
CDS	160..2961
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRKLHKAFLFGPCLQKH

Target ORF information:

RefSeq Version	XM_025287674.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287674.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGAAGCTA 
CACAAAGCCT TCTTGTTTGG ACCTTGTCTC CAGAAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_025287675.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143460.1 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu67928
Clone ID Related Accession (Same CDS sequence)	XM_025287675.1
Accession Version	XM_025287675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGGTACATTC GCTGCCAGAG AGAGGCGGGG AAGAGCTTTG 
AGCGGCATAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143460.1
CDS	160..2874
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTGTFAARERRGRALSGIS

Target ORF information:

RefSeq Version	XM_025287675.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287675.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGGTACATTC GCTGCCAGAG AGAGGCGGGG AAGAGCTTTG 
AGCGGCATAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_025287676.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143461.1 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu67929
Clone ID Related Accession (Same CDS sequence)	XM_025287676.1
Accession Version	XM_025287676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGGG CACAGATGGC ATGCCACCGA TGTCTGGTGT ACCTGGGGGA TTTGTCACGG 
TATCAGAATG AACTAGCTGG CGTAGACACT GAGCTGCTAG CTGAGAGATT TTACTACCAA 
GCCCTGTCAG TAGCTCCTCA GATTGGAATG CCCTTCAACC AGCTGGGCAC CCTTGCAGGC 
AGCAAGTACT ATAATGTGGA AGCCATGTAT TGCTACCTGC GCTGCATTCA GTCGGAAGTG 
TCCTTTGAGG GGGCCTATGG AAACCTCAAG CGGCTGTATG ACAAGGCAGC GAAAATGTAC 
CACCAGCTGA AGAAGTGTGA GACTCGGAAA CTCTCTCCCA GCAAGAAGCG ATGTAAAGAC 
ATTAAGAGGT TGCTGGTGAA CTTTATGTAT CTGCAAAGCC TCCTGCAGCC CAAAAGCAGC 
TCCGTGGACT CAGAGCTGAC ATCACTTTGC CAGTCAGTCC TGGAGGACTT CAACCTCTGT 
CTCTTCTACC TGCCCTCCTC ACCCAACCTC AGCCTGGCCA GTGAGGATGA GGAGGAGTAT 
GAGAGTGGAT ATGCTTTCCT CCCGGACCTT CTCATCTTTC AAATGGTCAT CATCTGCCTT 
ATGGGGGTGC ACAGCTTGAA GAGAGCAGGG TCCAAGCAGT ACAGTGCAGC CATTGCCTTC 
ACCCTGGCCC TCTTCTCCCA CCTCGTCAAT CACGTCAACA TCCGGCTGCA AGCTGAGCTG 
GAAGAGGGCG AGAATCCCGT CCCGGCGTTC CAGAGCGATG GCACAGATGA ACCGGAGTCC 
AGGGAACCCT TGGAGAAGGA GGAGGAGCCG GGGCCTGAGC CGCCTCCTGC AGTACCTCCA 
GAGGGTGAGG TCAGAAAGAG TCGGAAGTTC TCCCGCCTCT CCTGCCTCCG CCGCCGCCGC 
CACCCACCCA AAGCAGGTGA GGACAGTGAC CTGAGTGAAG GCTTTGACTC GGACTCAAGC 
CGTGACTCGG CCCGGGCCAG CGAGGGCTCG GATAGTGGCT CCGACAAGAG TCTTGAAGGT 
GGGGGAACAG CTTTTGACGC TGAGACAGAT TCAGAAATGA ACAGTCAAGA GTCCCGATCA 
GACCTGGAAG ATATGGAGGA TGAGGACGGG ACACGGTCCC CAGCCCTGGA GCCATCTCGG 
GCCAGGTCAG AGGCTCCTGA ATCCCTCAAT GGCCCTCTGG GCCCCAGCGA GGCCAGCATT 
GCCAGCAATC TACAAGCCAT GTCCACCCAG ATGTTCCAGA CCAAACGCTG CTTCCGACTG 
GCCCCCACCT TCAGCAACCT GCTCCTCCAG CCCACCACTG AAGCACCTGC CTCGGCCACC 
CTCAGGCGCT GTGTCAATGG AGATGTGGAC AAGCCTTCAG AGCCAGCCTC TGAAGAGGGC 
TCGGAGTCAG AGGGAAGCGA GTCCAGTGGG CGCTCCTTTC GGAACGAGCG GAGCATCCAG 
GAGAAGCTGC AGGTCTTGAT GGCTGAAGGC CTGCTCCCTG CTGTGAAAGT CTTCCTGGAC 
TGGCTGCGGA CCAACCCCGA CCTCATCGTT GTGTGTGCGC AGAGCTCTCA AAGTCTGTGG 
AACCGCCTGT CTGTGTTGTT GAACCTGTTG CCAGCTGCTG GCGAACTCCA AGAATCTGGC 
CTGGTCCTGT GTGCTGAGGT CCAGGAGCTG CTTGAAGGCT GTGAACTGCC TGACCTCCCA 
TCTAGCCTGC TGCTCCCAGA GGACACGGCC CTTCGCAACC TGCCTCCCCT CCGGGCAGCC 
CACAGACGCT TTAGCTTTGA TACAGCTCGG CTCTTGCTCA GCAATTTAGA GGAGACAGTC 
GTGCGGATCT GCTGCATACG CAGCTTTGGC CACTTCGTTG CCCGCCTGCA AGGCAGCATC 
CTGCAGTTCA ATGCAGAGGT CGGCATCTTT GTCAGCATCG CGCAGTCTGA GCAGGAGAGC 
CTGCTGCAGC AGGCCCAGGC CCAGTTCCGC ATGGCACAGG AGGAAGCTCG GCGGAACAGG 
CTGATGAGAG ACATGGCCCA GCTACGACTT CAGCTTGAGG TCTCTCAGCT GGAAGGGAGC 
CTGCAGCAGC CCAAGGCACG GTCAGCAATG TCTCCCTACC TCGTCCCCGA CACCCAGGCC 
CTCTGCCACC ACCTCCCTGT CATCCGCCAG CTGGCCACCA GTGGCCGCTT CATTGTCATC 
ATCCCAAGGA CAGTGATCGA TGGCCTGGAT TTGCTGAAGA AGGAACACCC AGGGGCCCGG 
GATGGGATCC GATATCTGGA GGCAGAGTTT AAAAAAGGGA ACAGGTACAT TCGCTGCCAG 
AGAGAGGCGG GGAAGAGCTT TGAGCGGCAT AAGCTGAAGA GGCAGGATGC CGATGCCTGG 
ACGCTCTATA AGATCCTGGA CAGCTGCAAA CAGCTGACTC TGGCCCAGGG AGCAGGTGAG 
GAGGACCCGA GTGGCATGGT GACCATCATC ACAGGCCTTC CACTGGACAA TCCTAGCACG 
CTCTCAGGCC CTATGCAGGC AGCCTTACAG GCCGCTGCAC GTGCCAGTGT GGACATCAAG 
AATGTTCTGG ACTTCTACAA GCAGTGGAAG GAGATTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143461.1
CDS	123..2684
Translation	MDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAARASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_025287676.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287676.1

ORF Insert Sequence:

ATGGATTGGG CACAGATGGC ATGCCACCGA TGTCTGGTGT ACCTGGGGGA TTTGTCACGG 
TATCAGAATG AACTAGCTGG CGTAGACACT GAGCTGCTAG CTGAGAGATT TTACTACCAA 
GCCCTGTCAG TAGCTCCTCA GATTGGAATG CCCTTCAACC AGCTGGGCAC CCTTGCAGGC 
AGCAAGTACT ATAATGTGGA AGCCATGTAT TGCTACCTGC GCTGCATTCA GTCGGAAGTG 
TCCTTTGAGG GGGCCTATGG AAACCTCAAG CGGCTGTATG ACAAGGCAGC GAAAATGTAC 
CACCAGCTGA AGAAGTGTGA GACTCGGAAA CTCTCTCCCA GCAAGAAGCG ATGTAAAGAC 
ATTAAGAGGT TGCTGGTGAA CTTTATGTAT CTGCAAAGCC TCCTGCAGCC CAAAAGCAGC 
TCCGTGGACT CAGAGCTGAC ATCACTTTGC CAGTCAGTCC TGGAGGACTT CAACCTCTGT 
CTCTTCTACC TGCCCTCCTC ACCCAACCTC AGCCTGGCCA GTGAGGATGA GGAGGAGTAT 
GAGAGTGGAT ATGCTTTCCT CCCGGACCTT CTCATCTTTC AAATGGTCAT CATCTGCCTT 
ATGGGGGTGC ACAGCTTGAA GAGAGCAGGG TCCAAGCAGT ACAGTGCAGC CATTGCCTTC 
ACCCTGGCCC TCTTCTCCCA CCTCGTCAAT CACGTCAACA TCCGGCTGCA AGCTGAGCTG 
GAAGAGGGCG AGAATCCCGT CCCGGCGTTC CAGAGCGATG GCACAGATGA ACCGGAGTCC 
AGGGAACCCT TGGAGAAGGA GGAGGAGCCG GGGCCTGAGC CGCCTCCTGC AGTACCTCCA 
GAGGGTGAGG TCAGAAAGAG TCGGAAGTTC TCCCGCCTCT CCTGCCTCCG CCGCCGCCGC 
CACCCACCCA AAGCAGGTGA GGACAGTGAC CTGAGTGAAG GCTTTGACTC GGACTCAAGC 
CGTGACTCGG CCCGGGCCAG CGAGGGCTCG GATAGTGGCT CCGACAAGAG TCTTGAAGGT 
GGGGGAACAG CTTTTGACGC TGAGACAGAT TCAGAAATGA ACAGTCAAGA GTCCCGATCA 
GACCTGGAAG ATATGGAGGA TGAGGACGGG ACACGGTCCC CAGCCCTGGA GCCATCTCGG 
GCCAGGTCAG AGGCTCCTGA ATCCCTCAAT GGCCCTCTGG GCCCCAGCGA GGCCAGCATT 
GCCAGCAATC TACAAGCCAT GTCCACCCAG ATGTTCCAGA CCAAACGCTG CTTCCGACTG 
GCCCCCACCT TCAGCAACCT GCTCCTCCAG CCCACCACTG AAGCACCTGC CTCGGCCACC 
CTCAGGCGCT GTGTCAATGG AGATGTGGAC AAGCCTTCAG AGCCAGCCTC TGAAGAGGGC 
TCGGAGTCAG AGGGAAGCGA GTCCAGTGGG CGCTCCTTTC GGAACGAGCG GAGCATCCAG 
GAGAAGCTGC AGGTCTTGAT GGCTGAAGGC CTGCTCCCTG CTGTGAAAGT CTTCCTGGAC 
TGGCTGCGGA CCAACCCCGA CCTCATCGTT GTGTGTGCGC AGAGCTCTCA AAGTCTGTGG 
AACCGCCTGT CTGTGTTGTT GAACCTGTTG CCAGCTGCTG GCGAACTCCA AGAATCTGGC 
CTGGTCCTGT GTGCTGAGGT CCAGGAGCTG CTTGAAGGCT GTGAACTGCC TGACCTCCCA 
TCTAGCCTGC TGCTCCCAGA GGACACGGCC CTTCGCAACC TGCCTCCCCT CCGGGCAGCC 
CACAGACGCT TTAGCTTTGA TACAGCTCGG CTCTTGCTCA GCAATTTAGA GGAGACAGTC 
GTGCGGATCT GCTGCATACG CAGCTTTGGC CACTTCGTTG CCCGCCTGCA AGGCAGCATC 
CTGCAGTTCA ATGCAGAGGT CGGCATCTTT GTCAGCATCG CGCAGTCTGA GCAGGAGAGC 
CTGCTGCAGC AGGCCCAGGC CCAGTTCCGC ATGGCACAGG AGGAAGCTCG GCGGAACAGG 
CTGATGAGAG ACATGGCCCA GCTACGACTT CAGCTTGAGG TCTCTCAGCT GGAAGGGAGC 
CTGCAGCAGC CCAAGGCACG GTCAGCAATG TCTCCCTACC TCGTCCCCGA CACCCAGGCC 
CTCTGCCACC ACCTCCCTGT CATCCGCCAG CTGGCCACCA GTGGCCGCTT CATTGTCATC 
ATCCCAAGGA CAGTGATCGA TGGCCTGGAT TTGCTGAAGA AGGAACACCC AGGGGCCCGG 
GATGGGATCC GATATCTGGA GGCAGAGTTT AAAAAAGGGA ACAGGTACAT TCGCTGCCAG 
AGAGAGGCGG GGAAGAGCTT TGAGCGGCAT AAGCTGAAGA GGCAGGATGC CGATGCCTGG 
ACGCTCTATA AGATCCTGGA CAGCTGCAAA CAGCTGACTC TGGCCCAGGG AGCAGGTGAG 
GAGGACCCGA GTGGCATGGT GACCATCATC ACAGGCCTTC CACTGGACAA TCCTAGCACG 
CTCTCAGGCC CTATGCAGGC AGCCTTACAG GCCGCTGCAC GTGCCAGTGT GGACATCAAG 
AATGTTCTGG ACTTCTACAA GCAGTGGAAG GAGATTGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_006051986.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052048.1 Bubalus bubalis SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.

Price & Availability↑

CloneID	OBu09776
Clone ID Related Accession (Same CDS sequence)	XM_006051986.1
Accession Version	XM_006051986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784415.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGG CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG CGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTACAGG CCGCTGCACA TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052048.1
CDS	153..3203
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGARSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_006051986.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5 nonsense mediated mRNA decay factor (SMG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051986.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGG CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG CGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTACAGG CCGCTGCACA TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_006051986.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052048.2 Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

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CloneID	OBu67924
Clone ID Related Accession (Same CDS sequence)	XM_006051986.2
Accession Version	XM_006051986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein SMG5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006051986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTACAGG CCGCTGCACG TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052048.2
CDS	159..3209
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESREPLEKEEEPGPEPPPAVPPEGEVRKSRKFSRLSCLRRRRHPPKAGEDSDLSEGFDSDSSRDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEDEDGTRSPALEPSRARSEAPESLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTEAPASATLRRCVNGDVDKPSEPASEEGSESEGSESSGRSFRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIVVCAQSSQSLWNRLSVLLNLLPAAGELQESGLVLCAEVQELLEGCELPDLPSSLLLPEDTALRNLPPLRAAHRRFSFDTARLLLSNLEETVVRICCIRSFGHFVARLQGSILQFNAEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKARSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQREAGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSTLSGPMQAALQAAARASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_006051986.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006051986.2

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCTG AAGCAAAGGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACCGCTT ATCAAGAGGT GTTCAAACCG GAGAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCGGTGGACT ATGGGAGGAA GGCTGAGGAG 
CTACTGTGGA GAAAGGTATA CTACGAGGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAT 
ATCCACAGCC GGAGTACCTT GGAATGTGCC TACAGGACAC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCTCACTACC AGCTGGAACT GCAATGCTGC 
ATCGACTGGA CCCATGTCAC CGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
TTGTCACGGT ATCAGAATGA ACTAGCTGGC GTAGACACTG AGCTGCTAGC TGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAACCA GCTGGGCACC 
CTTGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATTCAG 
TCGGAAGTGT CCTTTGAGGG GGCCTATGGA AACCTCAAGC GGCTGTATGA CAAGGCAGCG 
AAAATGTACC ACCAGCTGAA GAAGTGTGAG ACTCGGAAAC TCTCTCCCAG CAAGAAGCGA 
TGTAAAGACA TTAAGAGGTT GCTGGTGAAC TTTATGTATC TGCAAAGCCT CCTGCAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACA TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGTC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGGGGGTGCA CAGCTTGAAG AGAGCAGGGT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTCTCCCAC CTCGTCAATC ACGTCAACAT CCGGCTGCAA 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCGTTCC AGAGCGATGG CACAGATGAA 
CCGGAGTCCA GGGAACCCTT GGAGAAGGAG GAGGAGCCGG GGCCTGAGCC GCCTCCTGCA 
GTACCTCCAG AGGGTGAGGT CAGAAAGAGT CGGAAGTTCT CCCGCCTCTC CTGCCTCCGC 
CGCCGCCGCC ACCCACCCAA AGCAGGTGAG GACAGTGACC TGAGTGAAGG CTTTGACTCG 
GACTCAAGCC GTGACTCGGC CCGGGCCAGC GAGGGCTCGG ATAGTGGCTC CGACAAGAGT 
CTTGAAGGTG GGGGAACAGC TTTTGACGCT GAGACAGATT CAGAAATGAA CAGTCAAGAG 
TCCCGATCAG ACCTGGAAGA TATGGAGGAT GAGGACGGGA CACGGTCCCC AGCCCTGGAG 
CCATCTCGGG CCAGGTCAGA GGCTCCTGAA TCCCTCAATG GCCCTCTGGG CCCCAGCGAG 
GCCAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC CAAACGCTGC 
TTCCGACTGG CCCCCACCTT CAGCAACCTG CTCCTCCAGC CCACCACTGA AGCACCTGCC 
TCGGCCACCC TCAGGCGCTG TGTCAATGGA GATGTGGACA AGCCTTCAGA GCCAGCCTCT 
GAAGAGGGCT CGGAGTCAGA GGGAAGCGAG TCCAGTGGGC GCTCCTTTCG GAACGAGCGG 
AGCATCCAGG AGAAGCTGCA GGTCTTGATG GCTGAAGGCC TGCTCCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTGCGGAC CAACCCCGAC CTCATCGTTG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGTTG AACCTGTTGC CAGCTGCTGG CGAACTCCAA 
GAATCTGGCC TGGTCCTGTG TGCTGAGGTC CAGGAGCTGC TTGAAGGCTG TGAACTGCCT 
GACCTCCCAT CTAGCCTGCT GCTCCCAGAG GACACGGCCC TTCGCAACCT GCCTCCCCTC 
CGGGCAGCCC ACAGACGCTT TAGCTTTGAT ACAGCTCGGC TCTTGCTCAG CAATTTAGAG 
GAGACAGTCG TGCGGATCTG CTGCATACGC AGCTTTGGCC ACTTCGTTGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA TGCAGAGGTC GGCATCTTTG TCAGCATCGC GCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCC CAGTTCCGCA TGGCACAGGA GGAAGCTCGG 
CGGAACAGGC TGATGAGAGA CATGGCCCAG CTACGACTTC AGCTTGAGGT CTCTCAGCTG 
GAAGGGAGCC TGCAGCAGCC CAAGGCACGG TCAGCAATGT CTCCCTACCT CGTCCCCGAC 
ACCCAGGCCC TCTGCCACCA CCTCCCTGTC ATCCGCCAGC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATCCG ATATCTGGAG GCAGAGTTTA AAAAAGGGAA CAGGTACATT 
CGCTGCCAGA GAGAGGCGGG GAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCC 
GATGCCTGGA CGCTCTATAA GATCCTGGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGA 
GCAGGTGAGG AGGACCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAT 
CCTAGCACGC TCTCAGGCCC TATGCAGGCA GCCTTACAGG CCGCTGCACG TGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AGATTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SMG5 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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