CLCN4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCN4
Entrez Gene ID	102414747
Full Name	chloride voltage-gated channel 4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025276631.1	XP_025132416.1	H(+)/Cl(-) exchange transporter 4 isoform X1
XM_006050690.2	XP_006050752.1	H(+)/Cl(-) exchange transporter 4 isoform X1
XM_025276630.1	XP_025132415.1	H(+)/Cl(-) exchange transporter 4 isoform X1
XM_006050690.1	XP_006050752.1	H(+)/Cl(-) exchange transporter 4
XM_025276632.1	XP_025132417.1	H(+)/Cl(-) exchange transporter 4 isoform X2
XM_025276629.1	XP_025132414.1	H(+)/Cl(-) exchange transporter 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following CLCN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu08728	XM_025276629.1 Latest version!	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu08728	XM_025276631.1 Latest version!	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu08728	XM_025276630.1 Latest version!	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu08728	XM_006050690.2 Latest version!	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu73890	XM_025276632.1 Latest version!	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBu08728	XM_006050690.1	Bubalus bubalis chloride channel, voltage-sensitive 4 (CLCN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCN4 ( XM_025276631.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132416.1 Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu08728
Clone ID Related Accession (Same CDS sequence)	XM_025276631.1 , XM_006050690.2 , XM_025276630.1 , XM_006050690.1 , XM_025276629.1
Accession Version	XM_025276631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132416.1
CDS	498..2780
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSDSVMYFTEEPPELPANSPQPLKLRRVLNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_025276631.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276631.1

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN4 ( XM_006050690.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050752.1 Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu08728
Clone ID Related Accession (Same CDS sequence)	XM_025276631.1 , XM_006050690.2 , XM_025276630.1 , XM_006050690.1 , XM_025276629.1
Accession Version	XM_006050690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050752.1
CDS	369..2651
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSDSVMYFTEEPPELPANSPQPLKLRRVLNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_006050690.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050690.2

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN4 ( XM_025276630.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132415.1 Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu08728
Clone ID Related Accession (Same CDS sequence)	XM_025276631.1 , XM_006050690.2 , XM_025276630.1 , XM_006050690.1 , XM_025276629.1
Accession Version	XM_025276630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132415.1
CDS	494..2776
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSDSVMYFTEEPPELPANSPQPLKLRRVLNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_025276630.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276630.1

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN4 ( XM_006050690.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050752.1 Bubalus bubalis chloride channel, voltage-sensitive 4 (CLCN4), mRNA.

Price & Availability↑

CloneID	OBu08728
Clone ID Related Accession (Same CDS sequence)	XM_025276631.1 , XM_006050690.2 , XM_025276630.1 , XM_006050690.1 , XM_025276629.1
Accession Version	XM_006050690.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050752.1
CDS	411..2693
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSDSVMYFTEEPPELPANSPQPLKLRRVLNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_006050690.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride channel, voltage-sensitive 4 (CLCN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050690.1

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN4 ( XM_025276629.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132414.1 Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu08728
Clone ID Related Accession (Same CDS sequence)	XM_025276631.1 , XM_006050690.2 , XM_025276630.1 , XM_006050690.1 , XM_025276629.1
Accession Version	XM_025276629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132414.1
CDS	417..2699
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAIKNARQRQEGIVSDSVMYFTEEPPELPANSPQPLKLRRVLNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN

Target ORF information:

RefSeq Version	XM_025276629.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276629.1

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAAAGAAT 
GCCAGACAGA GGCAGGAGGG TATCGTAAGC GACTCGGTCA TGTACTTCAC CGAGGAGCCC 
CCCGAACTGC CGGCCAACAG CCCACAGCCC CTGAAGCTGC GGCGCGTCTT GAACCTCAGC 
CCTTTCACGG TCACCGACCA CACCCCTATG GAGACGGTGG TGGACATCTT CCGGAAGCTG 
GGGCTCCGCC AGTGCCTGGT GACCCGGAGC GGGAGACTTC TTGGCATCAT CACGAAAAAG 
GATGTTCTGA GACATATGGC CCAGATGGCA AACCAGGACC CTGAATCCAT CATGTTTAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN4 ( XM_025276632.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025132417.1 Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X5, mRNA.

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CloneID	OBu73890
Clone ID Related Accession (Same CDS sequence)	XM_025276632.1
Accession Version	XM_025276632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	H(+)/Cl(-) exchange transporter 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAACGTAT 
GGAAGAGGCT TGCTTACAAG GGCTCTCTGT CCAGAACTGA GCACCAAGAA ACGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025132417.1
CDS	417..2456
Translation	MVNTGVISGSGNLMDFLDEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWAVMLLIGLLAGTLAGVIDLAVDWMTDLKEGICLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFIRCNIAWCRRRKTTKLGKYPVLEVIAVTAITAIVAYPNPYTRRSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAIWQLALALIFKIIITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVSRDSERLIGFAQRRELILAITYGRGLLTRALCPELSTKKRV

Target ORF information:

RefSeq Version	XM_025276632.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chloride voltage-gated channel 4 (CLCN4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276632.1

ORF Insert Sequence:

ATGGTCAATA CAGGAGTGAT CAGTGGTTCT GGAAACCTGA TGGATTTCCT CGACGAGCCA 
TTCCCTGATG TGGGGACATA TGAGGACTTC CACACCATCG ACTGGCTGAG GGAAAAATCA 
CGGGACACCG ACAGACACAG GAAGATAACC AGCAAAAGCA AGGAGTCCAT ATGGGAGTTC 
ATCAAGAGTT TGTTGGATGC CTGGTCGGGA TGGGCAGTCA TGCTGCTCAT CGGCCTGCTG 
GCAGGCACTC TGGCCGGGGT CATCGATCTC GCTGTCGACT GGATGACCGA CCTGAAAGAG 
GGCATCTGCC TGTCCGCCTT CTGGTATAGC CATGAGCAGT GCTGCTGGAC TTCCAATGAG 
ACCACTTTTG AGGACAGGGA CAAGTGTCCC CTGTGGCAGA AGTGGTCAGA GCTGCTGGTG 
AATCAGTCGG AGGGTGCCAG TGCTTACATT CTGAATTACC TAATGTACAT CCTGTGGGCA 
TTGCTGTTTG CATTTCTGGC TGTCTCCCTG GTACGAGTAT TTGCACCATA TGCCTGTGGC 
TCTGGTATAC CCGAGATAAA GACCATCTTG AGTGGCTTCA TCATCAGGGG CTACTTGGGC 
AAGTGGACCC TGCTGATCAA GACTGTCACA CTGGTGCTGG TGGTGTCCTC AGGCCTGAGC 
CTGGGCAAGG AAGGGCCTCT AGTGCACGTG GCCTGTTGCT GCGGCAACTT CTTCAGCAGC 
CTTTTCTCCA AGTACAGCAA GAACGAGGGC AAAAGGCGTG AGGTGTTGTC AGCCGCGGCC 
GCTGCCGGGG TCTCCGTGGC CTTTGGTGCT CCAATTGGGG GTGTGCTTTT CAGTCTGGAA 
GAGGTCAGCT ACTACTTTCC CTTAAAGACC CTGTGGCGGT CCTTTTTTGC GGCCCTGGTG 
GCAGCCTTCA CACTGCGATC CATCAATCCA TTCGGGAACA GCCGCCTCGT CCTCTTCTAC 
GTGGAGTATC ACACGCCCTG GTACATGGCT GAGCTCTTCC CCTTCATCCT ACTTGGGGTC 
TTCGGGGGCT TGTGGGGGAC CCTCTTCATC CGCTGCAACA TCGCCTGGTG CCGGAGGCGC 
AAGACCACCA AGCTGGGGAA GTACCCAGTG TTGGAGGTCA TCGCGGTGAC TGCCATCACC 
GCCATTGTTG CCTACCCCAA CCCCTACACA CGCCGCAGCA CAAGCGAGCT CATTTCCGAG 
CTCTTCAATG ATTGCGGGGC CCTTGAGTCC TCCCAGCTGT GTGACTACAT CAACGACCCC 
AACATGACGC GGCCCGTGGA TGACATCCCC GACCGGCCAG CCGGGGTTGG GGTTTACACG 
GCCATCTGGC AGCTGGCCCT GGCGTTGATC TTCAAGATCA TCATTACCAT ATTCACCTTT 
GGCATGAAGA TCCCGTCCGG CCTCTTCATC CCCAGCATGG CTGTGGGGGC CATGGCGGGC 
AGGATGGTGG GAATCGGTGT GGAGCAGCTG GCTTACCATC ACCATGACTG GATCATCTTC 
AGGAACTGGT GCAGGCCCGG TGCAGACTGT GTCACCCCAG GGCTCTATGC CATGGTGGGG 
GCTGCAGCTT GCCTAGGTGG TGTTACCAGG ATGACGGTGT CATTGGTGGT CATCATGTTC 
GAATTGACAG GAGGCCTCGA GTACATCGTA CCCCTGATGG CAGCGGCCGT GACCAGCAAG 
TGGGTGGCCG ATGCCTTTGG GAAGGAAGGC ATCTACGAGG CCCACATCCA CTTGAACGGG 
TACCCGTTCC TGGATGTGAA GGACGAGTTC ACCCACCGCA CACTGGCCAC TGATGTCATG 
CGGCCGCGGC GGGGGGAGCC GCCCCTGTCC GTGCTCACCC AGGACAGCAT GACTGTCGAG 
GACGTGGAGA CACTCATCAA GGAGACTGAC TACAATGGCT TCCCTGTGGT CGTCTCCAGG 
GACTCAGAGC GCCTCATCGG CTTTGCCCAG AGGAGAGAGC TGATTCTTGC CATAACGTAT 
GGAAGAGGCT TGCTTACAAG GGCTCTCTGT CCAGAACTGA GCACCAAGAA ACGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CLCN4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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