CILP2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CILP2
Entrez Gene ID	102414004
Full Name	cartilage intermediate layer protein 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006080368.2	XP_006080430.1	cartilage intermediate layer protein 2 isoform X1
XM_025293925.1	XP_025149710.1	cartilage intermediate layer protein 2 isoform X3
XM_025293927.1	XP_025149712.1	cartilage intermediate layer protein 2 isoform X5
XM_006080368.1	XP_006080430.1	cartilage intermediate layer protein 2
XM_025293924.1	XP_025149709.1	cartilage intermediate layer protein 2 isoform X2
XM_025293926.1	XP_025149711.1	cartilage intermediate layer protein 2 isoform X4

Pathways from BioSystems
Gene Ontology

The following CILP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CILP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu73012	XM_025293925.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu73013	XM_025293926.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu73014	XM_025293927.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu73011	XM_025293924.1 Latest version!	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu33260	XM_006080368.2 Latest version!	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OBu33260	XM_006080368.1	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CILP2 ( XM_025293925.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149710.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu73012
Clone ID Related Accession (Same CDS sequence)	XM_025293925.1
Accession Version	XM_025293925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCCC AGCCAGGATG GTCATTTCTG CCCTCAAGAA AGCCCTCTTG GCTTGGGGCC 
ACCCCAGCCC ATCACTCAGA TGAGGAGACC AAGGTGTTCC CTCTGCCCGC TCTCCCCCGG 
AAGCAGAGGA CGGCGCTGGT CGTGTGCGGC CCAGAGCAGA ATGGAGCGGT GGGGCTGGCG 
TGTGCCCGGC ACCTGCGCGT GTTCGAGTAC GAACCCACCA TCTTCTACCC CACGCGCTCG 
CCGGACCCAC TGCACCGGGA CCTGACTATT CAGTGTGAGA AGATGGACAT CCCCTTCCTG 
TCCTATCTGC CCACAGAGGT CCAGCTCATC AACAACGCCT ACAGGCTAGT GGTGGACGCT 
GTGCTGGGCC CTGGTGTGGA GCCGGCCGAG GTCGGGGGTC CCTGCACACG CGCGCTGGCC 
ACGCTCAAGC TACTGTCCAT CCCCCTCGTG AGCCTGGACA TCCCCTCAGG CTGGGACCCA 
GAGACTGGCG GCGACGCCGA GGACGGGCTA CGACCCGACG TGCTCGTATC GCTGGCGGCG 
CCCAAGCGCT GCGCCGGCCG CTTCTCTGGG CGCCACCACT TCGTGGCGGG CAGGCTCTGT 
TCCAGGAGGC ACTTCCCGAC GTGGAGAAGG GAGGGTGGGC TCAGGAAGGG ACTTCCAGTC 
GGCGACGAAG GGACGCAGGC CAGCCCGAGG GCGGAGGTAC AGCCGGATGC ACCGAGGGGG 
TCGCCCCAGA GGGGTGGTCA CCCAAGTCAC CTCGGGCCCG GGAGTTGCCC AGACGGGGCG 
TGGCCTGCAT GGGGCGTGGC CTGCGGCCGC GTCCCTCCCC CATCCTCAGA CCCAGCGGCC 
GCCAGACCCG CGGGAGTCCG ACCCGAGTGC GCAGCGGAGC TGGGACCCTC CCTCCGACCT 
TCGGACCTGG CCATGGCGTC ACTGCCGACA CTGCTCTGCC TCTGTGTTGC CGCCGCACAC 
CTGGCGGGGG CCCGAGGTAC TCCGGACGCC GAGGAGCCCA CGGTGACCGC GTGGGGCCTT 
GAAGGTTCAT CGTTGCGCCC TGGCCAGCCC TCGCCAGCCC TGGAGGACTG GGAAGAGGCC 
AGCGAGTGGA CGTCCTGGTT CAACGTCGAC CACCCGGGCG GCGACGGTGA CTTCGAGAGC 
CTGGCCGCGA TCCGCTTCTA TTACGGGCCG GCACGCGTGT GCCCAAGGCC GCTGGCGCTG 
GAGGCGCGCA CCACAGACTG GGCGCTGCCT TCGGATGTCG GCGAGCGCGT ACACCTGAAC 
CCCACGCGCG GTTTCTGGTG CCTCAACCGC GAGCAGCCCC GAGGCCGCAG ATGCTCCAAT 
TACCACGTGC GCTTCCGCTG CCCGCTCGAG GCCACGTGGG GTTCGTGGGG CCCTTGGGGT 
CCCTGTTCGG GGAGCTGCGG GCCAGGCCGT CGCTTGCGCC GCCGCCGCTG CTCAAGCCCC 
GCGGGGGATG CGTGTCCCGG GCGTCCCTCG GAGGCGCAGA AGTGTGTGAG GCCTCGGTGT 
CCAGGGTGTG GCCCCAACAC CTGTGGGTGC CCCGACCACA TTCTTCTGGG CTCGGTGGTC 
ACCCCATCTG GGCGTCCACT GCCAGGAGCC AGGGTCTCCT TGAGAGACTG GCCTGGCACC 
ATAGCCACTA GTGATGCCCA TGGAACCTTC CGGATGCCTG GAGTCTGTGC CAGCAGCCGA 
GCCAACGTCA GTGCCCAAAT GGACGGCTTT TCAGCAGGCA TGGCCCAGAC CCAGGCCAAC 
AGCTCCATGT CAGCAGTGGC CACTGTTGTC CTTAAAAAGT TGGAGAAGCC CTACCTGGTG 
AAGCAACCCG AGTCTCGAGT TCGAGAGGCT GGCCAGAATG TGACCTTCTG CTGCAAAGCC 
TCAGGCACCC CCATGCCCAA GAAATACTCC TGGTTCCACA ACGGGACGCT GCTGGACAGG 
CGGACACACA GGTATGGGGC CCACCTGGAG CTGCATGGGC TCCGCCCAGA CCAAGCAGGC 
ACCTATCACT GCAAAGCGTG GAATGACGCG GGGGCCGTGC GATCGGGCAC TGCCCGACTC 
ACCGTACTTG CCCCGGGCCA GCCAGCCTGT GACCCCCGGC CCCGAGAACA CCTGATCAAG 
CTTCCAGATG ACTGTGGTCA GCCAGGCAGA GGGCCCACCT ACCTGGACGT AGGCCTTTGC 
CCTGACACCC TCTGCCCCAG CCCTGCAGGC TCCAGGCCCC GGTGTGGGGA CGCAGGCTCC 
CGCTGCTGCT CCGTGCGCCG CCTGGAGAGC AGGAAGATCC ACTGCTCTGG CTACACCCTC 
CCAGTCAAGA TCGTGGCTGA GTGTGGCTGT CAGAAATGTC TGCCCCTTCG GGGGCTGATC 
CGGGGACGCG TGATGGCCGC TGACTCTAGG GAGCCCTTGC GCTTTGCTCG CATCCTGCTG 
GGCCGGGAGC CCATTGGCTT CACGTCCTAC CAGGGTGACT TCACCCTCGA GGTGGCGCCT 
TCCACTCAGC GGCTGGTGAT GACCTTTGTG GACCCCAGTG GCCAGTTCGT GGACACTGTC 
AGGGTCCTGC CTTTTGACCC TCGAGGTGCT GGCGTGTACC ATGAGGTCAA GGCCATGCGA 
AAGAAAGCCC CCATCATTTT AGACGCCCGC CAGAGCAACA CCATATCCCT CGGGGAAATG 
GGAGAGGAGG CCCCCTTAGG AGAGCTGATC ATCCCGCCCG GGGCCTTCCG CAGAGCTGAC 
GGTCAACCCT ACATGGGGGC TGTGGAGGCC CAGGTGACCT TCGTGGACCC CCGAAATCTC 
ACTTCGGCAG CCGCCGCGCC CAGCGACCTG CGGTTCGTGG ACGGGGACGG GGAGCTGGCC 
CCGCTGCGCA CGTACGGCAT GTTCTCGGTG GACCTTCGGG CCGCCGGCTC CGCCGAGCAG 
CTGCAGACGG GGCTGGTGGC TGTGCGCGTG GCCGCTGACC AGATCCGGAT GCCGGGCCAC 
GTGGAGGCCC TGAAGCTGTG GTCGCTGAAC CCTGACACGG GCTTGTGGGA AGAAGAAAGC 
GGCTTCCAGC GCGAGAGACC GGCGTCCCAG CGGGCCCGCC GGGAGGAGCG GGTCTTCCTG 
GTGGGCAACG TGGAGATCCG CGAGCGCCGT CTGTTCAACC TGGACGTGCC CGAGCGCCGC 
CGCTGCTTCG TGAAGGTGCG CGCCTATGCC AACGACAAGT TCACCCCCAG CGAGCAGGTG 
GAGGGCGTGG TGGTCACGCT GGTCAATCTG GAGCCCGCCC CCGGCTTCTC TGCCAACCCT 
CGCGCCTGGG GCCGCTTCGA CAGCGCGGTC ACCGGCCCGA ACGGCGCCTG CCTCCCCGCC 
TTCTGCGACG CCGACCAGCC CGACGCCTAC ACCGCCGTGG TCACGGCCAC CCTGGGCGGG 
GAGGAGCTGG AGCCCGCGCC CTCGCGGCCG CGCCCGCTCC CGGCCACCGT TGGCGTGGCG 
CAGCCCTACC TGGACCGGCT GGCCTACCGC CGCACGGACC ATGACGACCC TGCCCTCAAG 
CGCAGTGGCT TCCGCATCAA CCTCGCCAAG CCCAGGCCCG GCGAACCCAC CGAGGCCAAC 
GGGCCCGTGT ACCCGTGGCG CAGCCTGCGG GAGTGCCAGG AGGCCCCGGT GACCGCCAGT 
CACTTCCGCT TCGCTCGTGT GGAGGCGGAC AAATACGAGT ACAACGTGGT CCCCTTCCGC 
GAGGGTGCGC CAGCCTCCTG GACCGGAGAT CTCCTGGCCT GGTGGCCCAA CCCGCAGGAG 
TTCCGGGCCT GCTTCCTCAA GGTGAAGATC CAAGGACCCC AGGAGTACAT GGTCCGCTCC 
CACAATTTGG GAGGCAGCCA TCCCCGGACC CAGGGCCAGC TCTACGGGCT GCGGGATTCC 
CGGAGTGTCC GGGACCCCCA GAGCCCTAGC TCCTCTGCCG CCTGTGTGGA ATTCAAGTGC 
AGCGGGATGC TGTTTGACCA GCGTCAGGTG GACAGGACGC TGGTGACCAT CATGCCGCAA 
GGCAGCTGCC GGCGTGTGGC GGTCAACGGG CTCCTGCAGG ATTACCTGAC ACGGCACCCC 
CCACCCGCGC CCGCTGATGA CCTGGCTTCC TTCTCCATGC TGGCCCCGCT GGACCCTCTG 
GGTCACAACT ATGGTGTCTA CACGGTCACC GACCAGAGCC CAAGGCTGGC CAAGGAGATC 
GCCATTGGCC GCTGCTTCGA CGGCTCCTCC GACGGCTTCT CCAGGGAGAT GAAGGCTGAC 
GCGGGCACAG CCGTCACCTT CCAGTGCCGG GAGCCAACTG CGGGCCGGCC CAGCCTCTTC 
CAGAGGCTGC TGGAGTCCCC ATCGGCGGCT CTTGGGGACA TCCGCAGGGA GATGGGCGAG 
GTGGCGCGCG TACAGGCCCG AGCCTCGAGT CCACTCCGCA CCCGCCGAGG CAGGGCCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149710.1
CDS	706..5091
Translation	MTPQPGWSFLPSRKPSWLGATPAHHSDEETKVFPLPALPRKQRTALVVCGPEQNGAVGLACARHLRVFEYEPTIFYPTRSPDPLHRDLTIQCEKMDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_025293925.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293925.1

ORF Insert Sequence:

ATGACCCCCC AGCCAGGATG GTCATTTCTG CCCTCAAGAA AGCCCTCTTG GCTTGGGGCC 
ACCCCAGCCC ATCACTCAGA TGAGGAGACC AAGGTGTTCC CTCTGCCCGC TCTCCCCCGG 
AAGCAGAGGA CGGCGCTGGT CGTGTGCGGC CCAGAGCAGA ATGGAGCGGT GGGGCTGGCG 
TGTGCCCGGC ACCTGCGCGT GTTCGAGTAC GAACCCACCA TCTTCTACCC CACGCGCTCG 
CCGGACCCAC TGCACCGGGA CCTGACTATT CAGTGTGAGA AGATGGACAT CCCCTTCCTG 
TCCTATCTGC CCACAGAGGT CCAGCTCATC AACAACGCCT ACAGGCTAGT GGTGGACGCT 
GTGCTGGGCC CTGGTGTGGA GCCGGCCGAG GTCGGGGGTC CCTGCACACG CGCGCTGGCC 
ACGCTCAAGC TACTGTCCAT CCCCCTCGTG AGCCTGGACA TCCCCTCAGG CTGGGACCCA 
GAGACTGGCG GCGACGCCGA GGACGGGCTA CGACCCGACG TGCTCGTATC GCTGGCGGCG 
CCCAAGCGCT GCGCCGGCCG CTTCTCTGGG CGCCACCACT TCGTGGCGGG CAGGCTCTGT 
TCCAGGAGGC ACTTCCCGAC GTGGAGAAGG GAGGGTGGGC TCAGGAAGGG ACTTCCAGTC 
GGCGACGAAG GGACGCAGGC CAGCCCGAGG GCGGAGGTAC AGCCGGATGC ACCGAGGGGG 
TCGCCCCAGA GGGGTGGTCA CCCAAGTCAC CTCGGGCCCG GGAGTTGCCC AGACGGGGCG 
TGGCCTGCAT GGGGCGTGGC CTGCGGCCGC GTCCCTCCCC CATCCTCAGA CCCAGCGGCC 
GCCAGACCCG CGGGAGTCCG ACCCGAGTGC GCAGCGGAGC TGGGACCCTC CCTCCGACCT 
TCGGACCTGG CCATGGCGTC ACTGCCGACA CTGCTCTGCC TCTGTGTTGC CGCCGCACAC 
CTGGCGGGGG CCCGAGGTAC TCCGGACGCC GAGGAGCCCA CGGTGACCGC GTGGGGCCTT 
GAAGGTTCAT CGTTGCGCCC TGGCCAGCCC TCGCCAGCCC TGGAGGACTG GGAAGAGGCC 
AGCGAGTGGA CGTCCTGGTT CAACGTCGAC CACCCGGGCG GCGACGGTGA CTTCGAGAGC 
CTGGCCGCGA TCCGCTTCTA TTACGGGCCG GCACGCGTGT GCCCAAGGCC GCTGGCGCTG 
GAGGCGCGCA CCACAGACTG GGCGCTGCCT TCGGATGTCG GCGAGCGCGT ACACCTGAAC 
CCCACGCGCG GTTTCTGGTG CCTCAACCGC GAGCAGCCCC GAGGCCGCAG ATGCTCCAAT 
TACCACGTGC GCTTCCGCTG CCCGCTCGAG GCCACGTGGG GTTCGTGGGG CCCTTGGGGT 
CCCTGTTCGG GGAGCTGCGG GCCAGGCCGT CGCTTGCGCC GCCGCCGCTG CTCAAGCCCC 
GCGGGGGATG CGTGTCCCGG GCGTCCCTCG GAGGCGCAGA AGTGTGTGAG GCCTCGGTGT 
CCAGGGTGTG GCCCCAACAC CTGTGGGTGC CCCGACCACA TTCTTCTGGG CTCGGTGGTC 
ACCCCATCTG GGCGTCCACT GCCAGGAGCC AGGGTCTCCT TGAGAGACTG GCCTGGCACC 
ATAGCCACTA GTGATGCCCA TGGAACCTTC CGGATGCCTG GAGTCTGTGC CAGCAGCCGA 
GCCAACGTCA GTGCCCAAAT GGACGGCTTT TCAGCAGGCA TGGCCCAGAC CCAGGCCAAC 
AGCTCCATGT CAGCAGTGGC CACTGTTGTC CTTAAAAAGT TGGAGAAGCC CTACCTGGTG 
AAGCAACCCG AGTCTCGAGT TCGAGAGGCT GGCCAGAATG TGACCTTCTG CTGCAAAGCC 
TCAGGCACCC CCATGCCCAA GAAATACTCC TGGTTCCACA ACGGGACGCT GCTGGACAGG 
CGGACACACA GGTATGGGGC CCACCTGGAG CTGCATGGGC TCCGCCCAGA CCAAGCAGGC 
ACCTATCACT GCAAAGCGTG GAATGACGCG GGGGCCGTGC GATCGGGCAC TGCCCGACTC 
ACCGTACTTG CCCCGGGCCA GCCAGCCTGT GACCCCCGGC CCCGAGAACA CCTGATCAAG 
CTTCCAGATG ACTGTGGTCA GCCAGGCAGA GGGCCCACCT ACCTGGACGT AGGCCTTTGC 
CCTGACACCC TCTGCCCCAG CCCTGCAGGC TCCAGGCCCC GGTGTGGGGA CGCAGGCTCC 
CGCTGCTGCT CCGTGCGCCG CCTGGAGAGC AGGAAGATCC ACTGCTCTGG CTACACCCTC 
CCAGTCAAGA TCGTGGCTGA GTGTGGCTGT CAGAAATGTC TGCCCCTTCG GGGGCTGATC 
CGGGGACGCG TGATGGCCGC TGACTCTAGG GAGCCCTTGC GCTTTGCTCG CATCCTGCTG 
GGCCGGGAGC CCATTGGCTT CACGTCCTAC CAGGGTGACT TCACCCTCGA GGTGGCGCCT 
TCCACTCAGC GGCTGGTGAT GACCTTTGTG GACCCCAGTG GCCAGTTCGT GGACACTGTC 
AGGGTCCTGC CTTTTGACCC TCGAGGTGCT GGCGTGTACC ATGAGGTCAA GGCCATGCGA 
AAGAAAGCCC CCATCATTTT AGACGCCCGC CAGAGCAACA CCATATCCCT CGGGGAAATG 
GGAGAGGAGG CCCCCTTAGG AGAGCTGATC ATCCCGCCCG GGGCCTTCCG CAGAGCTGAC 
GGTCAACCCT ACATGGGGGC TGTGGAGGCC CAGGTGACCT TCGTGGACCC CCGAAATCTC 
ACTTCGGCAG CCGCCGCGCC CAGCGACCTG CGGTTCGTGG ACGGGGACGG GGAGCTGGCC 
CCGCTGCGCA CGTACGGCAT GTTCTCGGTG GACCTTCGGG CCGCCGGCTC CGCCGAGCAG 
CTGCAGACGG GGCTGGTGGC TGTGCGCGTG GCCGCTGACC AGATCCGGAT GCCGGGCCAC 
GTGGAGGCCC TGAAGCTGTG GTCGCTGAAC CCTGACACGG GCTTGTGGGA AGAAGAAAGC 
GGCTTCCAGC GCGAGAGACC GGCGTCCCAG CGGGCCCGCC GGGAGGAGCG GGTCTTCCTG 
GTGGGCAACG TGGAGATCCG CGAGCGCCGT CTGTTCAACC TGGACGTGCC CGAGCGCCGC 
CGCTGCTTCG TGAAGGTGCG CGCCTATGCC AACGACAAGT TCACCCCCAG CGAGCAGGTG 
GAGGGCGTGG TGGTCACGCT GGTCAATCTG GAGCCCGCCC CCGGCTTCTC TGCCAACCCT 
CGCGCCTGGG GCCGCTTCGA CAGCGCGGTC ACCGGCCCGA ACGGCGCCTG CCTCCCCGCC 
TTCTGCGACG CCGACCAGCC CGACGCCTAC ACCGCCGTGG TCACGGCCAC CCTGGGCGGG 
GAGGAGCTGG AGCCCGCGCC CTCGCGGCCG CGCCCGCTCC CGGCCACCGT TGGCGTGGCG 
CAGCCCTACC TGGACCGGCT GGCCTACCGC CGCACGGACC ATGACGACCC TGCCCTCAAG 
CGCAGTGGCT TCCGCATCAA CCTCGCCAAG CCCAGGCCCG GCGAACCCAC CGAGGCCAAC 
GGGCCCGTGT ACCCGTGGCG CAGCCTGCGG GAGTGCCAGG AGGCCCCGGT GACCGCCAGT 
CACTTCCGCT TCGCTCGTGT GGAGGCGGAC AAATACGAGT ACAACGTGGT CCCCTTCCGC 
GAGGGTGCGC CAGCCTCCTG GACCGGAGAT CTCCTGGCCT GGTGGCCCAA CCCGCAGGAG 
TTCCGGGCCT GCTTCCTCAA GGTGAAGATC CAAGGACCCC AGGAGTACAT GGTCCGCTCC 
CACAATTTGG GAGGCAGCCA TCCCCGGACC CAGGGCCAGC TCTACGGGCT GCGGGATTCC 
CGGAGTGTCC GGGACCCCCA GAGCCCTAGC TCCTCTGCCG CCTGTGTGGA ATTCAAGTGC 
AGCGGGATGC TGTTTGACCA GCGTCAGGTG GACAGGACGC TGGTGACCAT CATGCCGCAA 
GGCAGCTGCC GGCGTGTGGC GGTCAACGGG CTCCTGCAGG ATTACCTGAC ACGGCACCCC 
CCACCCGCGC CCGCTGATGA CCTGGCTTCC TTCTCCATGC TGGCCCCGCT GGACCCTCTG 
GGTCACAACT ATGGTGTCTA CACGGTCACC GACCAGAGCC CAAGGCTGGC CAAGGAGATC 
GCCATTGGCC GCTGCTTCGA CGGCTCCTCC GACGGCTTCT CCAGGGAGAT GAAGGCTGAC 
GCGGGCACAG CCGTCACCTT CCAGTGCCGG GAGCCAACTG CGGGCCGGCC CAGCCTCTTC 
CAGAGGCTGC TGGAGTCCCC ATCGGCGGCT CTTGGGGACA TCCGCAGGGA GATGGGCGAG 
GTGGCGCGCG TACAGGCCCG AGCCTCGAGT CCACTCCGCA CCCGCCGAGG CAGGGCCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP2 ( XM_025293926.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149711.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu73013
Clone ID Related Accession (Same CDS sequence)	XM_025293926.1
Accession Version	XM_025293926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCA AAGCCACAAA AGCAAACCCA GTGTTCCCTC TGCCCGCTCT CCCCCGGAAG 
CAGAGGACGG CGCTGGTCGT GTGCGGCCCA GAGCAGAATG GAGCGGTGGG GCTGGCGTGT 
GCCCGGCACC TGCGCGTGTT CGAGTACGAA CCCACCATCT TCTACCCCAC GCGCTCGCCG 
GACCCACTGC ACCGGGACCT GACTATTCAG TGTGAGAAGA TGGACATCCC CTTCCTGTCC 
TATCTGCCCA CAGAGGTCCA GCTCATCAAC AACGCCTACA GGCTAGTGGT GGACGCTGTG 
CTGGGCCCTG GTGTGGAGCC GGCCGAGGTC GGGGGTCCCT GCACACGCGC GCTGGCCACG 
CTCAAGCTAC TGTCCATCCC CCTCGTGAGC CTGGACATCC CCTCAGGCTG GGACCCAGAG 
ACTGGCGGCG ACGCCGAGGA CGGGCTACGA CCCGACGTGC TCGTATCGCT GGCGGCGCCC 
AAGCGCTGCG CCGGCCGCTT CTCTGGGCGC CACCACTTCG TGGCGGGCAG GCTCTGTTCC 
AGGAGGCACT TCCCGACGTG GAGAAGGGAG GGTGGGCTCA GGAAGGGACT TCCAGTCGGC 
GACGAAGGGA CGCAGGCCAG CCCGAGGGCG GAGGTACAGC CGGATGCACC GAGGGGGTCG 
CCCCAGAGGG GTGGTCACCC AAGTCACCTC GGGCCCGGGA GTTGCCCAGA CGGGGCGTGG 
CCTGCATGGG GCGTGGCCTG CGGCCGCGTC CCTCCCCCAT CCTCAGACCC AGCGGCCGCC 
AGACCCGCGG GAGTCCGACC CGAGTGCGCA GCGGAGCTGG GACCCTCCCT CCGACCTTCG 
GACCTGGCCA TGGCGTCACT GCCGACACTG CTCTGCCTCT GTGTTGCCGC CGCACACCTG 
GCGGGGGCCC GAGGTACTCC GGACGCCGAG GAGCCCACGG TGACCGCGTG GGGCCTTGAA 
GGTTCATCGT TGCGCCCTGG CCAGCCCTCG CCAGCCCTGG AGGACTGGGA AGAGGCCAGC 
GAGTGGACGT CCTGGTTCAA CGTCGACCAC CCGGGCGGCG ACGGTGACTT CGAGAGCCTG 
GCCGCGATCC GCTTCTATTA CGGGCCGGCA CGCGTGTGCC CAAGGCCGCT GGCGCTGGAG 
GCGCGCACCA CAGACTGGGC GCTGCCTTCG GATGTCGGCG AGCGCGTACA CCTGAACCCC 
ACGCGCGGTT TCTGGTGCCT CAACCGCGAG CAGCCCCGAG GCCGCAGATG CTCCAATTAC 
CACGTGCGCT TCCGCTGCCC GCTCGAGGCC ACGTGGGGTT CGTGGGGCCC TTGGGGTCCC 
TGTTCGGGGA GCTGCGGGCC AGGCCGTCGC TTGCGCCGCC GCCGCTGCTC AAGCCCCGCG 
GGGGATGCGT GTCCCGGGCG TCCCTCGGAG GCGCAGAAGT GTGTGAGGCC TCGGTGTCCA 
GGGTGTGGCC CCAACACCTG TGGGTGCCCC GACCACATTC TTCTGGGCTC GGTGGTCACC 
CCATCTGGGC GTCCACTGCC AGGAGCCAGG GTCTCCTTGA GAGACTGGCC TGGCACCATA 
GCCACTAGTG ATGCCCATGG AACCTTCCGG ATGCCTGGAG TCTGTGCCAG CAGCCGAGCC 
AACGTCAGTG CCCAAATGGA CGGCTTTTCA GCAGGCATGG CCCAGACCCA GGCCAACAGC 
TCCATGTCAG CAGTGGCCAC TGTTGTCCTT AAAAAGTTGG AGAAGCCCTA CCTGGTGAAG 
CAACCCGAGT CTCGAGTTCG AGAGGCTGGC CAGAATGTGA CCTTCTGCTG CAAAGCCTCA 
GGCACCCCCA TGCCCAAGAA ATACTCCTGG TTCCACAACG GGACGCTGCT GGACAGGCGG 
ACACACAGGT ATGGGGCCCA CCTGGAGCTG CATGGGCTCC GCCCAGACCA AGCAGGCACC 
TATCACTGCA AAGCGTGGAA TGACGCGGGG GCCGTGCGAT CGGGCACTGC CCGACTCACC 
GTACTTGCCC CGGGCCAGCC AGCCTGTGAC CCCCGGCCCC GAGAACACCT GATCAAGCTT 
CCAGATGACT GTGGTCAGCC AGGCAGAGGG CCCACCTACC TGGACGTAGG CCTTTGCCCT 
GACACCCTCT GCCCCAGCCC TGCAGGCTCC AGGCCCCGGT GTGGGGACGC AGGCTCCCGC 
TGCTGCTCCG TGCGCCGCCT GGAGAGCAGG AAGATCCACT GCTCTGGCTA CACCCTCCCA 
GTCAAGATCG TGGCTGAGTG TGGCTGTCAG AAATGTCTGC CCCTTCGGGG GCTGATCCGG 
GGACGCGTGA TGGCCGCTGA CTCTAGGGAG CCCTTGCGCT TTGCTCGCAT CCTGCTGGGC 
CGGGAGCCCA TTGGCTTCAC GTCCTACCAG GGTGACTTCA CCCTCGAGGT GGCGCCTTCC 
ACTCAGCGGC TGGTGATGAC CTTTGTGGAC CCCAGTGGCC AGTTCGTGGA CACTGTCAGG 
GTCCTGCCTT TTGACCCTCG AGGTGCTGGC GTGTACCATG AGGTCAAGGC CATGCGAAAG 
AAAGCCCCCA TCATTTTAGA CGCCCGCCAG AGCAACACCA TATCCCTCGG GGAAATGGGA 
GAGGAGGCCC CCTTAGGAGA GCTGATCATC CCGCCCGGGG CCTTCCGCAG AGCTGACGGT 
CAACCCTACA TGGGGGCTGT GGAGGCCCAG GTGACCTTCG TGGACCCCCG AAATCTCACT 
TCGGCAGCCG CCGCGCCCAG CGACCTGCGG TTCGTGGACG GGGACGGGGA GCTGGCCCCG 
CTGCGCACGT ACGGCATGTT CTCGGTGGAC CTTCGGGCCG CCGGCTCCGC CGAGCAGCTG 
CAGACGGGGC TGGTGGCTGT GCGCGTGGCC GCTGACCAGA TCCGGATGCC GGGCCACGTG 
GAGGCCCTGA AGCTGTGGTC GCTGAACCCT GACACGGGCT TGTGGGAAGA AGAAAGCGGC 
TTCCAGCGCG AGAGACCGGC GTCCCAGCGG GCCCGCCGGG AGGAGCGGGT CTTCCTGGTG 
GGCAACGTGG AGATCCGCGA GCGCCGTCTG TTCAACCTGG ACGTGCCCGA GCGCCGCCGC 
TGCTTCGTGA AGGTGCGCGC CTATGCCAAC GACAAGTTCA CCCCCAGCGA GCAGGTGGAG 
GGCGTGGTGG TCACGCTGGT CAATCTGGAG CCCGCCCCCG GCTTCTCTGC CAACCCTCGC 
GCCTGGGGCC GCTTCGACAG CGCGGTCACC GGCCCGAACG GCGCCTGCCT CCCCGCCTTC 
TGCGACGCCG ACCAGCCCGA CGCCTACACC GCCGTGGTCA CGGCCACCCT GGGCGGGGAG 
GAGCTGGAGC CCGCGCCCTC GCGGCCGCGC CCGCTCCCGG CCACCGTTGG CGTGGCGCAG 
CCCTACCTGG ACCGGCTGGC CTACCGCCGC ACGGACCATG ACGACCCTGC CCTCAAGCGC 
AGTGGCTTCC GCATCAACCT CGCCAAGCCC AGGCCCGGCG AACCCACCGA GGCCAACGGG 
CCCGTGTACC CGTGGCGCAG CCTGCGGGAG TGCCAGGAGG CCCCGGTGAC CGCCAGTCAC 
TTCCGCTTCG CTCGTGTGGA GGCGGACAAA TACGAGTACA ACGTGGTCCC CTTCCGCGAG 
GGTGCGCCAG CCTCCTGGAC CGGAGATCTC CTGGCCTGGT GGCCCAACCC GCAGGAGTTC 
CGGGCCTGCT TCCTCAAGGT GAAGATCCAA GGACCCCAGG AGTACATGGT CCGCTCCCAC 
AATTTGGGAG GCAGCCATCC CCGGACCCAG GGCCAGCTCT ACGGGCTGCG GGATTCCCGG 
AGTGTCCGGG ACCCCCAGAG CCCTAGCTCC TCTGCCGCCT GTGTGGAATT CAAGTGCAGC 
GGGATGCTGT TTGACCAGCG TCAGGTGGAC AGGACGCTGG TGACCATCAT GCCGCAAGGC 
AGCTGCCGGC GTGTGGCGGT CAACGGGCTC CTGCAGGATT ACCTGACACG GCACCCCCCA 
CCCGCGCCCG CTGATGACCT GGCTTCCTTC TCCATGCTGG CCCCGCTGGA CCCTCTGGGT 
CACAACTATG GTGTCTACAC GGTCACCGAC CAGAGCCCAA GGCTGGCCAA GGAGATCGCC 
ATTGGCCGCT GCTTCGACGG CTCCTCCGAC GGCTTCTCCA GGGAGATGAA GGCTGACGCG 
GGCACAGCCG TCACCTTCCA GTGCCGGGAG CCAACTGCGG GCCGGCCCAG CCTCTTCCAG 
AGGCTGCTGG AGTCCCCATC GGCGGCTCTT GGGGACATCC GCAGGGAGAT GGGCGAGGTG 
GCGCGCGTAC AGGCCCGAGC CTCGAGTCCA CTCCGCACCC GCCGAGGCAG GGCCCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149711.1
CDS	678..5000
Translation	MKIKATKANPVFPLPALPRKQRTALVVCGPEQNGAVGLACARHLRVFEYEPTIFYPTRSPDPLHRDLTIQCEKMDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_025293926.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293926.1

ORF Insert Sequence:

ATGAAAATCA AAGCCACAAA AGCAAACCCA GTGTTCCCTC TGCCCGCTCT CCCCCGGAAG 
CAGAGGACGG CGCTGGTCGT GTGCGGCCCA GAGCAGAATG GAGCGGTGGG GCTGGCGTGT 
GCCCGGCACC TGCGCGTGTT CGAGTACGAA CCCACCATCT TCTACCCCAC GCGCTCGCCG 
GACCCACTGC ACCGGGACCT GACTATTCAG TGTGAGAAGA TGGACATCCC CTTCCTGTCC 
TATCTGCCCA CAGAGGTCCA GCTCATCAAC AACGCCTACA GGCTAGTGGT GGACGCTGTG 
CTGGGCCCTG GTGTGGAGCC GGCCGAGGTC GGGGGTCCCT GCACACGCGC GCTGGCCACG 
CTCAAGCTAC TGTCCATCCC CCTCGTGAGC CTGGACATCC CCTCAGGCTG GGACCCAGAG 
ACTGGCGGCG ACGCCGAGGA CGGGCTACGA CCCGACGTGC TCGTATCGCT GGCGGCGCCC 
AAGCGCTGCG CCGGCCGCTT CTCTGGGCGC CACCACTTCG TGGCGGGCAG GCTCTGTTCC 
AGGAGGCACT TCCCGACGTG GAGAAGGGAG GGTGGGCTCA GGAAGGGACT TCCAGTCGGC 
GACGAAGGGA CGCAGGCCAG CCCGAGGGCG GAGGTACAGC CGGATGCACC GAGGGGGTCG 
CCCCAGAGGG GTGGTCACCC AAGTCACCTC GGGCCCGGGA GTTGCCCAGA CGGGGCGTGG 
CCTGCATGGG GCGTGGCCTG CGGCCGCGTC CCTCCCCCAT CCTCAGACCC AGCGGCCGCC 
AGACCCGCGG GAGTCCGACC CGAGTGCGCA GCGGAGCTGG GACCCTCCCT CCGACCTTCG 
GACCTGGCCA TGGCGTCACT GCCGACACTG CTCTGCCTCT GTGTTGCCGC CGCACACCTG 
GCGGGGGCCC GAGGTACTCC GGACGCCGAG GAGCCCACGG TGACCGCGTG GGGCCTTGAA 
GGTTCATCGT TGCGCCCTGG CCAGCCCTCG CCAGCCCTGG AGGACTGGGA AGAGGCCAGC 
GAGTGGACGT CCTGGTTCAA CGTCGACCAC CCGGGCGGCG ACGGTGACTT CGAGAGCCTG 
GCCGCGATCC GCTTCTATTA CGGGCCGGCA CGCGTGTGCC CAAGGCCGCT GGCGCTGGAG 
GCGCGCACCA CAGACTGGGC GCTGCCTTCG GATGTCGGCG AGCGCGTACA CCTGAACCCC 
ACGCGCGGTT TCTGGTGCCT CAACCGCGAG CAGCCCCGAG GCCGCAGATG CTCCAATTAC 
CACGTGCGCT TCCGCTGCCC GCTCGAGGCC ACGTGGGGTT CGTGGGGCCC TTGGGGTCCC 
TGTTCGGGGA GCTGCGGGCC AGGCCGTCGC TTGCGCCGCC GCCGCTGCTC AAGCCCCGCG 
GGGGATGCGT GTCCCGGGCG TCCCTCGGAG GCGCAGAAGT GTGTGAGGCC TCGGTGTCCA 
GGGTGTGGCC CCAACACCTG TGGGTGCCCC GACCACATTC TTCTGGGCTC GGTGGTCACC 
CCATCTGGGC GTCCACTGCC AGGAGCCAGG GTCTCCTTGA GAGACTGGCC TGGCACCATA 
GCCACTAGTG ATGCCCATGG AACCTTCCGG ATGCCTGGAG TCTGTGCCAG CAGCCGAGCC 
AACGTCAGTG CCCAAATGGA CGGCTTTTCA GCAGGCATGG CCCAGACCCA GGCCAACAGC 
TCCATGTCAG CAGTGGCCAC TGTTGTCCTT AAAAAGTTGG AGAAGCCCTA CCTGGTGAAG 
CAACCCGAGT CTCGAGTTCG AGAGGCTGGC CAGAATGTGA CCTTCTGCTG CAAAGCCTCA 
GGCACCCCCA TGCCCAAGAA ATACTCCTGG TTCCACAACG GGACGCTGCT GGACAGGCGG 
ACACACAGGT ATGGGGCCCA CCTGGAGCTG CATGGGCTCC GCCCAGACCA AGCAGGCACC 
TATCACTGCA AAGCGTGGAA TGACGCGGGG GCCGTGCGAT CGGGCACTGC CCGACTCACC 
GTACTTGCCC CGGGCCAGCC AGCCTGTGAC CCCCGGCCCC GAGAACACCT GATCAAGCTT 
CCAGATGACT GTGGTCAGCC AGGCAGAGGG CCCACCTACC TGGACGTAGG CCTTTGCCCT 
GACACCCTCT GCCCCAGCCC TGCAGGCTCC AGGCCCCGGT GTGGGGACGC AGGCTCCCGC 
TGCTGCTCCG TGCGCCGCCT GGAGAGCAGG AAGATCCACT GCTCTGGCTA CACCCTCCCA 
GTCAAGATCG TGGCTGAGTG TGGCTGTCAG AAATGTCTGC CCCTTCGGGG GCTGATCCGG 
GGACGCGTGA TGGCCGCTGA CTCTAGGGAG CCCTTGCGCT TTGCTCGCAT CCTGCTGGGC 
CGGGAGCCCA TTGGCTTCAC GTCCTACCAG GGTGACTTCA CCCTCGAGGT GGCGCCTTCC 
ACTCAGCGGC TGGTGATGAC CTTTGTGGAC CCCAGTGGCC AGTTCGTGGA CACTGTCAGG 
GTCCTGCCTT TTGACCCTCG AGGTGCTGGC GTGTACCATG AGGTCAAGGC CATGCGAAAG 
AAAGCCCCCA TCATTTTAGA CGCCCGCCAG AGCAACACCA TATCCCTCGG GGAAATGGGA 
GAGGAGGCCC CCTTAGGAGA GCTGATCATC CCGCCCGGGG CCTTCCGCAG AGCTGACGGT 
CAACCCTACA TGGGGGCTGT GGAGGCCCAG GTGACCTTCG TGGACCCCCG AAATCTCACT 
TCGGCAGCCG CCGCGCCCAG CGACCTGCGG TTCGTGGACG GGGACGGGGA GCTGGCCCCG 
CTGCGCACGT ACGGCATGTT CTCGGTGGAC CTTCGGGCCG CCGGCTCCGC CGAGCAGCTG 
CAGACGGGGC TGGTGGCTGT GCGCGTGGCC GCTGACCAGA TCCGGATGCC GGGCCACGTG 
GAGGCCCTGA AGCTGTGGTC GCTGAACCCT GACACGGGCT TGTGGGAAGA AGAAAGCGGC 
TTCCAGCGCG AGAGACCGGC GTCCCAGCGG GCCCGCCGGG AGGAGCGGGT CTTCCTGGTG 
GGCAACGTGG AGATCCGCGA GCGCCGTCTG TTCAACCTGG ACGTGCCCGA GCGCCGCCGC 
TGCTTCGTGA AGGTGCGCGC CTATGCCAAC GACAAGTTCA CCCCCAGCGA GCAGGTGGAG 
GGCGTGGTGG TCACGCTGGT CAATCTGGAG CCCGCCCCCG GCTTCTCTGC CAACCCTCGC 
GCCTGGGGCC GCTTCGACAG CGCGGTCACC GGCCCGAACG GCGCCTGCCT CCCCGCCTTC 
TGCGACGCCG ACCAGCCCGA CGCCTACACC GCCGTGGTCA CGGCCACCCT GGGCGGGGAG 
GAGCTGGAGC CCGCGCCCTC GCGGCCGCGC CCGCTCCCGG CCACCGTTGG CGTGGCGCAG 
CCCTACCTGG ACCGGCTGGC CTACCGCCGC ACGGACCATG ACGACCCTGC CCTCAAGCGC 
AGTGGCTTCC GCATCAACCT CGCCAAGCCC AGGCCCGGCG AACCCACCGA GGCCAACGGG 
CCCGTGTACC CGTGGCGCAG CCTGCGGGAG TGCCAGGAGG CCCCGGTGAC CGCCAGTCAC 
TTCCGCTTCG CTCGTGTGGA GGCGGACAAA TACGAGTACA ACGTGGTCCC CTTCCGCGAG 
GGTGCGCCAG CCTCCTGGAC CGGAGATCTC CTGGCCTGGT GGCCCAACCC GCAGGAGTTC 
CGGGCCTGCT TCCTCAAGGT GAAGATCCAA GGACCCCAGG AGTACATGGT CCGCTCCCAC 
AATTTGGGAG GCAGCCATCC CCGGACCCAG GGCCAGCTCT ACGGGCTGCG GGATTCCCGG 
AGTGTCCGGG ACCCCCAGAG CCCTAGCTCC TCTGCCGCCT GTGTGGAATT CAAGTGCAGC 
GGGATGCTGT TTGACCAGCG TCAGGTGGAC AGGACGCTGG TGACCATCAT GCCGCAAGGC 
AGCTGCCGGC GTGTGGCGGT CAACGGGCTC CTGCAGGATT ACCTGACACG GCACCCCCCA 
CCCGCGCCCG CTGATGACCT GGCTTCCTTC TCCATGCTGG CCCCGCTGGA CCCTCTGGGT 
CACAACTATG GTGTCTACAC GGTCACCGAC CAGAGCCCAA GGCTGGCCAA GGAGATCGCC 
ATTGGCCGCT GCTTCGACGG CTCCTCCGAC GGCTTCTCCA GGGAGATGAA GGCTGACGCG 
GGCACAGCCG TCACCTTCCA GTGCCGGGAG CCAACTGCGG GCCGGCCCAG CCTCTTCCAG 
AGGCTGCTGG AGTCCCCATC GGCGGCTCTT GGGGACATCC GCAGGGAGAT GGGCGAGGTG 
GCGCGCGTAC AGGCCCGAGC CTCGAGTCCA CTCCGCACCC GCCGAGGCAG GGCCCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP2 ( XM_025293927.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149712.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X5, mRNA.

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CloneID	OBu73014
Clone ID Related Accession (Same CDS sequence)	XM_025293927.1
Accession Version	XM_025293927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATCC CCTTCCTGTC CTATCTGCCC ACAGAGGTCC AGCTCATCAA CAACGCCTAC 
AGGCTAGTGG TGGACGCTGT GCTGGGCCCT GGTGTGGAGC CGGCCGAGGT CGGGGGTCCC 
TGCACACGCG CGCTGGCCAC GCTCAAGCTA CTGTCCATCC CCCTCGTGAG CCTGGACATC 
CCCTCAGGCT GGGACCCAGA GACTGGCGGC GACGCCGAGG ACGGGCTACG ACCCGACGTG 
CTCGTATCGC TGGCGGCGCC CAAGCGCTGC GCCGGCCGCT TCTCTGGGCG CCACCACTTC 
GTGGCGGGCA GGCTCTGTTC CAGGAGGCAC TTCCCGACGT GGAGAAGGGA GGGTGGGCTC 
AGGAAGGGAC TTCCAGTCGG CGACGAAGGG ACGCAGGCCA GCCCGAGGGC GGAGGTACAG 
CCGGATGCAC CGAGGGGGTC GCCCCAGAGG GGTGGTCACC CAAGTCACCT CGGGCCCGGG 
AGTTGCCCAG ACGGGGCGTG GCCTGCATGG GGCGTGGCCT GCGGCCGCGT CCCTCCCCCA 
TCCTCAGACC CAGCGGCCGC CAGACCCGCG GGAGTCCGAC CCGAGTGCGC AGCGGAGCTG 
GGACCCTCCC TCCGACCTTC GGACCTGGCC ATGGCGTCAC TGCCGACACT GCTCTGCCTC 
TGTGTTGCCG CCGCACACCT GGCGGGGGCC CGAGGTACTC CGGACGCCGA GGAGCCCACG 
GTGACCGCGT GGGGCCTTGA AGGTTCATCG TTGCGCCCTG GCCAGCCCTC GCCAGCCCTG 
GAGGACTGGG AAGAGGCCAG CGAGTGGACG TCCTGGTTCA ACGTCGACCA CCCGGGCGGC 
GACGGTGACT TCGAGAGCCT GGCCGCGATC CGCTTCTATT ACGGGCCGGC ACGCGTGTGC 
CCAAGGCCGC TGGCGCTGGA GGCGCGCACC ACAGACTGGG CGCTGCCTTC GGATGTCGGC 
GAGCGCGTAC ACCTGAACCC CACGCGCGGT TTCTGGTGCC TCAACCGCGA GCAGCCCCGA 
GGCCGCAGAT GCTCCAATTA CCACGTGCGC TTCCGCTGCC CGCTCGAGGC CACGTGGGGT 
TCGTGGGGCC CTTGGGGTCC CTGTTCGGGG AGCTGCGGGC CAGGCCGTCG CTTGCGCCGC 
CGCCGCTGCT CAAGCCCCGC GGGGGATGCG TGTCCCGGGC GTCCCTCGGA GGCGCAGAAG 
TGTGTGAGGC CTCGGTGTCC AGGGTGTGGC CCCAACACCT GTGGGTGCCC CGACCACATT 
CTTCTGGGCT CGGTGGTCAC CCCATCTGGG CGTCCACTGC CAGGAGCCAG GGTCTCCTTG 
AGAGACTGGC CTGGCACCAT AGCCACTAGT GATGCCCATG GAACCTTCCG GATGCCTGGA 
GTCTGTGCCA GCAGCCGAGC CAACGTCAGT GCCCAAATGG ACGGCTTTTC AGCAGGCATG 
GCCCAGACCC AGGCCAACAG CTCCATGTCA GCAGTGGCCA CTGTTGTCCT TAAAAAGTTG 
GAGAAGCCCT ACCTGGTGAA GCAACCCGAG TCTCGAGTTC GAGAGGCTGG CCAGAATGTG 
ACCTTCTGCT GCAAAGCCTC AGGCACCCCC ATGCCCAAGA AATACTCCTG GTTCCACAAC 
GGGACGCTGC TGGACAGGCG GACACACAGG TATGGGGCCC ACCTGGAGCT GCATGGGCTC 
CGCCCAGACC AAGCAGGCAC CTATCACTGC AAAGCGTGGA ATGACGCGGG GGCCGTGCGA 
TCGGGCACTG CCCGACTCAC CGTACTTGCC CCGGGCCAGC CAGCCTGTGA CCCCCGGCCC 
CGAGAACACC TGATCAAGCT TCCAGATGAC TGTGGTCAGC CAGGCAGAGG GCCCACCTAC 
CTGGACGTAG GCCTTTGCCC TGACACCCTC TGCCCCAGCC CTGCAGGCTC CAGGCCCCGG 
TGTGGGGACG CAGGCTCCCG CTGCTGCTCC GTGCGCCGCC TGGAGAGCAG GAAGATCCAC 
TGCTCTGGCT ACACCCTCCC AGTCAAGATC GTGGCTGAGT GTGGCTGTCA GAAATGTCTG 
CCCCTTCGGG GGCTGATCCG GGGACGCGTG ATGGCCGCTG ACTCTAGGGA GCCCTTGCGC 
TTTGCTCGCA TCCTGCTGGG CCGGGAGCCC ATTGGCTTCA CGTCCTACCA GGGTGACTTC 
ACCCTCGAGG TGGCGCCTTC CACTCAGCGG CTGGTGATGA CCTTTGTGGA CCCCAGTGGC 
CAGTTCGTGG ACACTGTCAG GGTCCTGCCT TTTGACCCTC GAGGTGCTGG CGTGTACCAT 
GAGGTCAAGG CCATGCGAAA GAAAGCCCCC ATCATTTTAG ACGCCCGCCA GAGCAACACC 
ATATCCCTCG GGGAAATGGG AGAGGAGGCC CCCTTAGGAG AGCTGATCAT CCCGCCCGGG 
GCCTTCCGCA GAGCTGACGG TCAACCCTAC ATGGGGGCTG TGGAGGCCCA GGTGACCTTC 
GTGGACCCCC GAAATCTCAC TTCGGCAGCC GCCGCGCCCA GCGACCTGCG GTTCGTGGAC 
GGGGACGGGG AGCTGGCCCC GCTGCGCACG TACGGCATGT TCTCGGTGGA CCTTCGGGCC 
GCCGGCTCCG CCGAGCAGCT GCAGACGGGG CTGGTGGCTG TGCGCGTGGC CGCTGACCAG 
ATCCGGATGC CGGGCCACGT GGAGGCCCTG AAGCTGTGGT CGCTGAACCC TGACACGGGC 
TTGTGGGAAG AAGAAAGCGG CTTCCAGCGC GAGAGACCGG CGTCCCAGCG GGCCCGCCGG 
GAGGAGCGGG TCTTCCTGGT GGGCAACGTG GAGATCCGCG AGCGCCGTCT GTTCAACCTG 
GACGTGCCCG AGCGCCGCCG CTGCTTCGTG AAGGTGCGCG CCTATGCCAA CGACAAGTTC 
ACCCCCAGCG AGCAGGTGGA GGGCGTGGTG GTCACGCTGG TCAATCTGGA GCCCGCCCCC 
GGCTTCTCTG CCAACCCTCG CGCCTGGGGC CGCTTCGACA GCGCGGTCAC CGGCCCGAAC 
GGCGCCTGCC TCCCCGCCTT CTGCGACGCC GACCAGCCCG ACGCCTACAC CGCCGTGGTC 
ACGGCCACCC TGGGCGGGGA GGAGCTGGAG CCCGCGCCCT CGCGGCCGCG CCCGCTCCCG 
GCCACCGTTG GCGTGGCGCA GCCCTACCTG GACCGGCTGG CCTACCGCCG CACGGACCAT 
GACGACCCTG CCCTCAAGCG CAGTGGCTTC CGCATCAACC TCGCCAAGCC CAGGCCCGGC 
GAACCCACCG AGGCCAACGG GCCCGTGTAC CCGTGGCGCA GCCTGCGGGA GTGCCAGGAG 
GCCCCGGTGA CCGCCAGTCA CTTCCGCTTC GCTCGTGTGG AGGCGGACAA ATACGAGTAC 
AACGTGGTCC CCTTCCGCGA GGGTGCGCCA GCCTCCTGGA CCGGAGATCT CCTGGCCTGG 
TGGCCCAACC CGCAGGAGTT CCGGGCCTGC TTCCTCAAGG TGAAGATCCA AGGACCCCAG 
GAGTACATGG TCCGCTCCCA CAATTTGGGA GGCAGCCATC CCCGGACCCA GGGCCAGCTC 
TACGGGCTGC GGGATTCCCG GAGTGTCCGG GACCCCCAGA GCCCTAGCTC CTCTGCCGCC 
TGTGTGGAAT TCAAGTGCAG CGGGATGCTG TTTGACCAGC GTCAGGTGGA CAGGACGCTG 
GTGACCATCA TGCCGCAAGG CAGCTGCCGG CGTGTGGCGG TCAACGGGCT CCTGCAGGAT 
TACCTGACAC GGCACCCCCC ACCCGCGCCC GCTGATGACC TGGCTTCCTT CTCCATGCTG 
GCCCCGCTGG ACCCTCTGGG TCACAACTAT GGTGTCTACA CGGTCACCGA CCAGAGCCCA 
AGGCTGGCCA AGGAGATCGC CATTGGCCGC TGCTTCGACG GCTCCTCCGA CGGCTTCTCC 
AGGGAGATGA AGGCTGACGC GGGCACAGCC GTCACCTTCC AGTGCCGGGA GCCAACTGCG 
GGCCGGCCCA GCCTCTTCCA GAGGCTGCTG GAGTCCCCAT CGGCGGCTCT TGGGGACATC 
CGCAGGGAGA TGGGCGAGGT GGCGCGCGTA CAGGCCCGAG CCTCGAGTCC ACTCCGCACC 
CGCCGAGGCA GGGCCCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149712.1
CDS	716..4819
Translation	MDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_025293927.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293927.1

ORF Insert Sequence:

ATGGACATCC CCTTCCTGTC CTATCTGCCC ACAGAGGTCC AGCTCATCAA CAACGCCTAC 
AGGCTAGTGG TGGACGCTGT GCTGGGCCCT GGTGTGGAGC CGGCCGAGGT CGGGGGTCCC 
TGCACACGCG CGCTGGCCAC GCTCAAGCTA CTGTCCATCC CCCTCGTGAG CCTGGACATC 
CCCTCAGGCT GGGACCCAGA GACTGGCGGC GACGCCGAGG ACGGGCTACG ACCCGACGTG 
CTCGTATCGC TGGCGGCGCC CAAGCGCTGC GCCGGCCGCT TCTCTGGGCG CCACCACTTC 
GTGGCGGGCA GGCTCTGTTC CAGGAGGCAC TTCCCGACGT GGAGAAGGGA GGGTGGGCTC 
AGGAAGGGAC TTCCAGTCGG CGACGAAGGG ACGCAGGCCA GCCCGAGGGC GGAGGTACAG 
CCGGATGCAC CGAGGGGGTC GCCCCAGAGG GGTGGTCACC CAAGTCACCT CGGGCCCGGG 
AGTTGCCCAG ACGGGGCGTG GCCTGCATGG GGCGTGGCCT GCGGCCGCGT CCCTCCCCCA 
TCCTCAGACC CAGCGGCCGC CAGACCCGCG GGAGTCCGAC CCGAGTGCGC AGCGGAGCTG 
GGACCCTCCC TCCGACCTTC GGACCTGGCC ATGGCGTCAC TGCCGACACT GCTCTGCCTC 
TGTGTTGCCG CCGCACACCT GGCGGGGGCC CGAGGTACTC CGGACGCCGA GGAGCCCACG 
GTGACCGCGT GGGGCCTTGA AGGTTCATCG TTGCGCCCTG GCCAGCCCTC GCCAGCCCTG 
GAGGACTGGG AAGAGGCCAG CGAGTGGACG TCCTGGTTCA ACGTCGACCA CCCGGGCGGC 
GACGGTGACT TCGAGAGCCT GGCCGCGATC CGCTTCTATT ACGGGCCGGC ACGCGTGTGC 
CCAAGGCCGC TGGCGCTGGA GGCGCGCACC ACAGACTGGG CGCTGCCTTC GGATGTCGGC 
GAGCGCGTAC ACCTGAACCC CACGCGCGGT TTCTGGTGCC TCAACCGCGA GCAGCCCCGA 
GGCCGCAGAT GCTCCAATTA CCACGTGCGC TTCCGCTGCC CGCTCGAGGC CACGTGGGGT 
TCGTGGGGCC CTTGGGGTCC CTGTTCGGGG AGCTGCGGGC CAGGCCGTCG CTTGCGCCGC 
CGCCGCTGCT CAAGCCCCGC GGGGGATGCG TGTCCCGGGC GTCCCTCGGA GGCGCAGAAG 
TGTGTGAGGC CTCGGTGTCC AGGGTGTGGC CCCAACACCT GTGGGTGCCC CGACCACATT 
CTTCTGGGCT CGGTGGTCAC CCCATCTGGG CGTCCACTGC CAGGAGCCAG GGTCTCCTTG 
AGAGACTGGC CTGGCACCAT AGCCACTAGT GATGCCCATG GAACCTTCCG GATGCCTGGA 
GTCTGTGCCA GCAGCCGAGC CAACGTCAGT GCCCAAATGG ACGGCTTTTC AGCAGGCATG 
GCCCAGACCC AGGCCAACAG CTCCATGTCA GCAGTGGCCA CTGTTGTCCT TAAAAAGTTG 
GAGAAGCCCT ACCTGGTGAA GCAACCCGAG TCTCGAGTTC GAGAGGCTGG CCAGAATGTG 
ACCTTCTGCT GCAAAGCCTC AGGCACCCCC ATGCCCAAGA AATACTCCTG GTTCCACAAC 
GGGACGCTGC TGGACAGGCG GACACACAGG TATGGGGCCC ACCTGGAGCT GCATGGGCTC 
CGCCCAGACC AAGCAGGCAC CTATCACTGC AAAGCGTGGA ATGACGCGGG GGCCGTGCGA 
TCGGGCACTG CCCGACTCAC CGTACTTGCC CCGGGCCAGC CAGCCTGTGA CCCCCGGCCC 
CGAGAACACC TGATCAAGCT TCCAGATGAC TGTGGTCAGC CAGGCAGAGG GCCCACCTAC 
CTGGACGTAG GCCTTTGCCC TGACACCCTC TGCCCCAGCC CTGCAGGCTC CAGGCCCCGG 
TGTGGGGACG CAGGCTCCCG CTGCTGCTCC GTGCGCCGCC TGGAGAGCAG GAAGATCCAC 
TGCTCTGGCT ACACCCTCCC AGTCAAGATC GTGGCTGAGT GTGGCTGTCA GAAATGTCTG 
CCCCTTCGGG GGCTGATCCG GGGACGCGTG ATGGCCGCTG ACTCTAGGGA GCCCTTGCGC 
TTTGCTCGCA TCCTGCTGGG CCGGGAGCCC ATTGGCTTCA CGTCCTACCA GGGTGACTTC 
ACCCTCGAGG TGGCGCCTTC CACTCAGCGG CTGGTGATGA CCTTTGTGGA CCCCAGTGGC 
CAGTTCGTGG ACACTGTCAG GGTCCTGCCT TTTGACCCTC GAGGTGCTGG CGTGTACCAT 
GAGGTCAAGG CCATGCGAAA GAAAGCCCCC ATCATTTTAG ACGCCCGCCA GAGCAACACC 
ATATCCCTCG GGGAAATGGG AGAGGAGGCC CCCTTAGGAG AGCTGATCAT CCCGCCCGGG 
GCCTTCCGCA GAGCTGACGG TCAACCCTAC ATGGGGGCTG TGGAGGCCCA GGTGACCTTC 
GTGGACCCCC GAAATCTCAC TTCGGCAGCC GCCGCGCCCA GCGACCTGCG GTTCGTGGAC 
GGGGACGGGG AGCTGGCCCC GCTGCGCACG TACGGCATGT TCTCGGTGGA CCTTCGGGCC 
GCCGGCTCCG CCGAGCAGCT GCAGACGGGG CTGGTGGCTG TGCGCGTGGC CGCTGACCAG 
ATCCGGATGC CGGGCCACGT GGAGGCCCTG AAGCTGTGGT CGCTGAACCC TGACACGGGC 
TTGTGGGAAG AAGAAAGCGG CTTCCAGCGC GAGAGACCGG CGTCCCAGCG GGCCCGCCGG 
GAGGAGCGGG TCTTCCTGGT GGGCAACGTG GAGATCCGCG AGCGCCGTCT GTTCAACCTG 
GACGTGCCCG AGCGCCGCCG CTGCTTCGTG AAGGTGCGCG CCTATGCCAA CGACAAGTTC 
ACCCCCAGCG AGCAGGTGGA GGGCGTGGTG GTCACGCTGG TCAATCTGGA GCCCGCCCCC 
GGCTTCTCTG CCAACCCTCG CGCCTGGGGC CGCTTCGACA GCGCGGTCAC CGGCCCGAAC 
GGCGCCTGCC TCCCCGCCTT CTGCGACGCC GACCAGCCCG ACGCCTACAC CGCCGTGGTC 
ACGGCCACCC TGGGCGGGGA GGAGCTGGAG CCCGCGCCCT CGCGGCCGCG CCCGCTCCCG 
GCCACCGTTG GCGTGGCGCA GCCCTACCTG GACCGGCTGG CCTACCGCCG CACGGACCAT 
GACGACCCTG CCCTCAAGCG CAGTGGCTTC CGCATCAACC TCGCCAAGCC CAGGCCCGGC 
GAACCCACCG AGGCCAACGG GCCCGTGTAC CCGTGGCGCA GCCTGCGGGA GTGCCAGGAG 
GCCCCGGTGA CCGCCAGTCA CTTCCGCTTC GCTCGTGTGG AGGCGGACAA ATACGAGTAC 
AACGTGGTCC CCTTCCGCGA GGGTGCGCCA GCCTCCTGGA CCGGAGATCT CCTGGCCTGG 
TGGCCCAACC CGCAGGAGTT CCGGGCCTGC TTCCTCAAGG TGAAGATCCA AGGACCCCAG 
GAGTACATGG TCCGCTCCCA CAATTTGGGA GGCAGCCATC CCCGGACCCA GGGCCAGCTC 
TACGGGCTGC GGGATTCCCG GAGTGTCCGG GACCCCCAGA GCCCTAGCTC CTCTGCCGCC 
TGTGTGGAAT TCAAGTGCAG CGGGATGCTG TTTGACCAGC GTCAGGTGGA CAGGACGCTG 
GTGACCATCA TGCCGCAAGG CAGCTGCCGG CGTGTGGCGG TCAACGGGCT CCTGCAGGAT 
TACCTGACAC GGCACCCCCC ACCCGCGCCC GCTGATGACC TGGCTTCCTT CTCCATGCTG 
GCCCCGCTGG ACCCTCTGGG TCACAACTAT GGTGTCTACA CGGTCACCGA CCAGAGCCCA 
AGGCTGGCCA AGGAGATCGC CATTGGCCGC TGCTTCGACG GCTCCTCCGA CGGCTTCTCC 
AGGGAGATGA AGGCTGACGC GGGCACAGCC GTCACCTTCC AGTGCCGGGA GCCAACTGCG 
GGCCGGCCCA GCCTCTTCCA GAGGCTGCTG GAGTCCCCAT CGGCGGCTCT TGGGGACATC 
CGCAGGGAGA TGGGCGAGGT GGCGCGCGTA CAGGCCCGAG CCTCGAGTCC ACTCCGCACC 
CGCCGAGGCA GGGCCCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP2 ( XM_025293924.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025149709.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X2, mRNA.

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CloneID	OBu73011
Clone ID Related Accession (Same CDS sequence)	XM_025293924.1
Accession Version	XM_025293924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT GGAAACATGG GCGGGGGAAG GGGCAGCCAC TGCAGCGGAA AAAGGTGGAA 
GAATGTCTGC AGCTCAGGCC GAGAATAAAC AAACTGCACT CTGGCTTGGG TGGTGGCAGG 
AGGCACGTTT CTCTGCTCAA GCTTGAGAGA GCCCGAGGCA AGGACTCTGG GTCTCCAACA 
AGGAAACTGA GGCCCCTAGG GGCCCCATGC CACCCCCAGA ACACCTCTCC CCGGTCTCGC 
CCTCTCGCCT CCTGGCCCGA GCCCCACTCC TTCCTCCACC CTGGCCTCAG TTTCCCCGCC 
GGCCTCGGGA GGCGTTCCGA ACTCCGCGCC TTTGCCTTTG CCGTGCCCTG CCAGGTGTTC 
CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG 
AATGGAGCGG TGGGGCTGGC GTGTGCCCGG CACCTGCGCG TGTTCGAGTA CGAACCCACC 
ATCTTCTACC CCACGCGCTC GCCGGACCCA CTGCACCGGG ACCTGACTAT TCAGTGTGAG 
AAGATGGACA TCCCCTTCCT GTCCTATCTG CCCACAGAGG TCCAGCTCAT CAACAACGCC 
TACAGGCTAG TGGTGGACGC TGTGCTGGGC CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT 
CCCTGCACAC GCGCGCTGGC CACGCTCAAG CTACTGTCCA TCCCCCTCGT GAGCCTGGAC 
ATCCCCTCAG GCTGGGACCC AGAGACTGGC GGCGACGCCG AGGACGGGCT ACGACCCGAC 
GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC TGCGCCGGCC GCTTCTCTGG GCGCCACCAC 
TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG CACTTCCCGA CGTGGAGAAG GGAGGGTGGG 
CTCAGGAAGG GACTTCCAGT CGGCGACGAA GGGACGCAGG CCAGCCCGAG GGCGGAGGTA 
CAGCCGGATG CACCGAGGGG GTCGCCCCAG AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC 
GGGAGTTGCC CAGACGGGGC GTGGCCTGCA TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC 
CCATCCTCAG ACCCAGCGGC CGCCAGACCC GCGGGAGTCC GACCCGAGTG CGCAGCGGAG 
CTGGGACCCT CCCTCCGACC TTCGGACCTG GCCATGGCGT CACTGCCGAC ACTGCTCTGC 
CTCTGTGTTG CCGCCGCACA CCTGGCGGGG GCCCGAGGTA CTCCGGACGC CGAGGAGCCC 
ACGGTGACCG CGTGGGGCCT TGAAGGTTCA TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC 
CTGGAGGACT GGGAAGAGGC CAGCGAGTGG ACGTCCTGGT TCAACGTCGA CCACCCGGGC 
GGCGACGGTG ACTTCGAGAG CCTGGCCGCG ATCCGCTTCT ATTACGGGCC GGCACGCGTG 
TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC ACCACAGACT GGGCGCTGCC TTCGGATGTC 
GGCGAGCGCG TACACCTGAA CCCCACGCGC GGTTTCTGGT GCCTCAACCG CGAGCAGCCC 
CGAGGCCGCA GATGCTCCAA TTACCACGTG CGCTTCCGCT GCCCGCTCGA GGCCACGTGG 
GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC 
CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG 
AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT GGCCCCAACA CCTGTGGGTG CCCCGACCAC 
ATTCTTCTGG GCTCGGTGGT CACCCCATCT GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC 
TTGAGAGACT GGCCTGGCAC CATAGCCACT AGTGATGCCC ATGGAACCTT CCGGATGCCT 
GGAGTCTGTG CCAGCAGCCG AGCCAACGTC AGTGCCCAAA TGGACGGCTT TTCAGCAGGC 
ATGGCCCAGA CCCAGGCCAA CAGCTCCATG TCAGCAGTGG CCACTGTTGT CCTTAAAAAG 
TTGGAGAAGC CCTACCTGGT GAAGCAACCC GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT 
GTGACCTTCT GCTGCAAAGC CTCAGGCACC CCCATGCCCA AGAAATACTC CTGGTTCCAC 
AACGGGACGC TGCTGGACAG GCGGACACAC AGGTATGGGG CCCACCTGGA GCTGCATGGG 
CTCCGCCCAG ACCAAGCAGG CACCTATCAC TGCAAAGCGT GGAATGACGC GGGGGCCGTG 
CGATCGGGCA CTGCCCGACT CACCGTACTT GCCCCGGGCC AGCCAGCCTG TGACCCCCGG 
CCCCGAGAAC ACCTGATCAA GCTTCCAGAT GACTGTGGTC AGCCAGGCAG AGGGCCCACC 
TACCTGGACG TAGGCCTTTG CCCTGACACC CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC 
CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC TCCGTGCGCC GCCTGGAGAG CAGGAAGATC 
CACTGCTCTG GCTACACCCT CCCAGTCAAG ATCGTGGCTG AGTGTGGCTG TCAGAAATGT 
CTGCCCCTTC GGGGGCTGAT CCGGGGACGC GTGATGGCCG CTGACTCTAG GGAGCCCTTG 
CGCTTTGCTC GCATCCTGCT GGGCCGGGAG CCCATTGGCT TCACGTCCTA CCAGGGTGAC 
TTCACCCTCG AGGTGGCGCC TTCCACTCAG CGGCTGGTGA TGACCTTTGT GGACCCCAGT 
GGCCAGTTCG TGGACACTGT CAGGGTCCTG CCTTTTGACC CTCGAGGTGC TGGCGTGTAC 
CATGAGGTCA AGGCCATGCG AAAGAAAGCC CCCATCATTT TAGACGCCCG CCAGAGCAAC 
ACCATATCCC TCGGGGAAAT GGGAGAGGAG GCCCCCTTAG GAGAGCTGAT CATCCCGCCC 
GGGGCCTTCC GCAGAGCTGA CGGTCAACCC TACATGGGGG CTGTGGAGGC CCAGGTGACC 
TTCGTGGACC CCCGAAATCT CACTTCGGCA GCCGCCGCGC CCAGCGACCT GCGGTTCGTG 
GACGGGGACG GGGAGCTGGC CCCGCTGCGC ACGTACGGCA TGTTCTCGGT GGACCTTCGG 
GCCGCCGGCT CCGCCGAGCA GCTGCAGACG GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC 
CAGATCCGGA TGCCGGGCCA CGTGGAGGCC CTGAAGCTGT GGTCGCTGAA CCCTGACACG 
GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC 
CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC GTGGAGATCC GCGAGCGCCG TCTGTTCAAC 
CTGGACGTGC CCGAGCGCCG CCGCTGCTTC GTGAAGGTGC GCGCCTATGC CAACGACAAG 
TTCACCCCCA GCGAGCAGGT GGAGGGCGTG GTGGTCACGC TGGTCAATCT GGAGCCCGCC 
CCCGGCTTCT CTGCCAACCC TCGCGCCTGG GGCCGCTTCG ACAGCGCGGT CACCGGCCCG 
AACGGCGCCT GCCTCCCCGC CTTCTGCGAC GCCGACCAGC CCGACGCCTA CACCGCCGTG 
GTCACGGCCA CCCTGGGCGG GGAGGAGCTG GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC 
CCGGCCACCG TTGGCGTGGC GCAGCCCTAC CTGGACCGGC TGGCCTACCG CCGCACGGAC 
CATGACGACC CTGCCCTCAA GCGCAGTGGC TTCCGCATCA ACCTCGCCAA GCCCAGGCCC 
GGCGAACCCA CCGAGGCCAA CGGGCCCGTG TACCCGTGGC GCAGCCTGCG GGAGTGCCAG 
GAGGCCCCGG TGACCGCCAG TCACTTCCGC TTCGCTCGTG TGGAGGCGGA CAAATACGAG 
TACAACGTGG TCCCCTTCCG CGAGGGTGCG CCAGCCTCCT GGACCGGAGA TCTCCTGGCC 
TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC TGCTTCCTCA AGGTGAAGAT CCAAGGACCC 
CAGGAGTACA TGGTCCGCTC CCACAATTTG GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG 
CTCTACGGGC TGCGGGATTC CCGGAGTGTC CGGGACCCCC AGAGCCCTAG CTCCTCTGCC 
GCCTGTGTGG AATTCAAGTG CAGCGGGATG CTGTTTGACC AGCGTCAGGT GGACAGGACG 
CTGGTGACCA TCATGCCGCA AGGCAGCTGC CGGCGTGTGG CGGTCAACGG GCTCCTGCAG 
GATTACCTGA CACGGCACCC CCCACCCGCG CCCGCTGATG ACCTGGCTTC CTTCTCCATG 
CTGGCCCCGC TGGACCCTCT GGGTCACAAC TATGGTGTCT ACACGGTCAC CGACCAGAGC 
CCAAGGCTGG CCAAGGAGAT CGCCATTGGC CGCTGCTTCG ACGGCTCCTC CGACGGCTTC 
TCCAGGGAGA TGAAGGCTGA CGCGGGCACA GCCGTCACCT TCCAGTGCCG GGAGCCAACT 
GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG CTGGAGTCCC CATCGGCGGC TCTTGGGGAC 
ATCCGCAGGG AGATGGGCGA GGTGGCGCGC GTACAGGCCC GAGCCTCGAG TCCACTCCGC 
ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025149709.1
CDS	1478..6124
Translation	MQAWKHGRGKGQPLQRKKVEECLQLRPRINKLHSGLGGGRRHVSLLKLERARGKDSGSPTRKLRPLGAPCHPQNTSPRSRPLASWPEPHSFLHPGLSFPAGLGRRSELRAFAFAVPCQVFPLPALPRKQRTALVVCGPEQNGAVGLACARHLRVFEYEPTIFYPTRSPDPLHRDLTIQCEKMDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_025293924.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025293924.1

ORF Insert Sequence:

ATGCAGGCCT GGAAACATGG GCGGGGGAAG GGGCAGCCAC TGCAGCGGAA AAAGGTGGAA 
GAATGTCTGC AGCTCAGGCC GAGAATAAAC AAACTGCACT CTGGCTTGGG TGGTGGCAGG 
AGGCACGTTT CTCTGCTCAA GCTTGAGAGA GCCCGAGGCA AGGACTCTGG GTCTCCAACA 
AGGAAACTGA GGCCCCTAGG GGCCCCATGC CACCCCCAGA ACACCTCTCC CCGGTCTCGC 
CCTCTCGCCT CCTGGCCCGA GCCCCACTCC TTCCTCCACC CTGGCCTCAG TTTCCCCGCC 
GGCCTCGGGA GGCGTTCCGA ACTCCGCGCC TTTGCCTTTG CCGTGCCCTG CCAGGTGTTC 
CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG 
AATGGAGCGG TGGGGCTGGC GTGTGCCCGG CACCTGCGCG TGTTCGAGTA CGAACCCACC 
ATCTTCTACC CCACGCGCTC GCCGGACCCA CTGCACCGGG ACCTGACTAT TCAGTGTGAG 
AAGATGGACA TCCCCTTCCT GTCCTATCTG CCCACAGAGG TCCAGCTCAT CAACAACGCC 
TACAGGCTAG TGGTGGACGC TGTGCTGGGC CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT 
CCCTGCACAC GCGCGCTGGC CACGCTCAAG CTACTGTCCA TCCCCCTCGT GAGCCTGGAC 
ATCCCCTCAG GCTGGGACCC AGAGACTGGC GGCGACGCCG AGGACGGGCT ACGACCCGAC 
GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC TGCGCCGGCC GCTTCTCTGG GCGCCACCAC 
TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG CACTTCCCGA CGTGGAGAAG GGAGGGTGGG 
CTCAGGAAGG GACTTCCAGT CGGCGACGAA GGGACGCAGG CCAGCCCGAG GGCGGAGGTA 
CAGCCGGATG CACCGAGGGG GTCGCCCCAG AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC 
GGGAGTTGCC CAGACGGGGC GTGGCCTGCA TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC 
CCATCCTCAG ACCCAGCGGC CGCCAGACCC GCGGGAGTCC GACCCGAGTG CGCAGCGGAG 
CTGGGACCCT CCCTCCGACC TTCGGACCTG GCCATGGCGT CACTGCCGAC ACTGCTCTGC 
CTCTGTGTTG CCGCCGCACA CCTGGCGGGG GCCCGAGGTA CTCCGGACGC CGAGGAGCCC 
ACGGTGACCG CGTGGGGCCT TGAAGGTTCA TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC 
CTGGAGGACT GGGAAGAGGC CAGCGAGTGG ACGTCCTGGT TCAACGTCGA CCACCCGGGC 
GGCGACGGTG ACTTCGAGAG CCTGGCCGCG ATCCGCTTCT ATTACGGGCC GGCACGCGTG 
TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC ACCACAGACT GGGCGCTGCC TTCGGATGTC 
GGCGAGCGCG TACACCTGAA CCCCACGCGC GGTTTCTGGT GCCTCAACCG CGAGCAGCCC 
CGAGGCCGCA GATGCTCCAA TTACCACGTG CGCTTCCGCT GCCCGCTCGA GGCCACGTGG 
GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC 
CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG 
AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT GGCCCCAACA CCTGTGGGTG CCCCGACCAC 
ATTCTTCTGG GCTCGGTGGT CACCCCATCT GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC 
TTGAGAGACT GGCCTGGCAC CATAGCCACT AGTGATGCCC ATGGAACCTT CCGGATGCCT 
GGAGTCTGTG CCAGCAGCCG AGCCAACGTC AGTGCCCAAA TGGACGGCTT TTCAGCAGGC 
ATGGCCCAGA CCCAGGCCAA CAGCTCCATG TCAGCAGTGG CCACTGTTGT CCTTAAAAAG 
TTGGAGAAGC CCTACCTGGT GAAGCAACCC GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT 
GTGACCTTCT GCTGCAAAGC CTCAGGCACC CCCATGCCCA AGAAATACTC CTGGTTCCAC 
AACGGGACGC TGCTGGACAG GCGGACACAC AGGTATGGGG CCCACCTGGA GCTGCATGGG 
CTCCGCCCAG ACCAAGCAGG CACCTATCAC TGCAAAGCGT GGAATGACGC GGGGGCCGTG 
CGATCGGGCA CTGCCCGACT CACCGTACTT GCCCCGGGCC AGCCAGCCTG TGACCCCCGG 
CCCCGAGAAC ACCTGATCAA GCTTCCAGAT GACTGTGGTC AGCCAGGCAG AGGGCCCACC 
TACCTGGACG TAGGCCTTTG CCCTGACACC CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC 
CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC TCCGTGCGCC GCCTGGAGAG CAGGAAGATC 
CACTGCTCTG GCTACACCCT CCCAGTCAAG ATCGTGGCTG AGTGTGGCTG TCAGAAATGT 
CTGCCCCTTC GGGGGCTGAT CCGGGGACGC GTGATGGCCG CTGACTCTAG GGAGCCCTTG 
CGCTTTGCTC GCATCCTGCT GGGCCGGGAG CCCATTGGCT TCACGTCCTA CCAGGGTGAC 
TTCACCCTCG AGGTGGCGCC TTCCACTCAG CGGCTGGTGA TGACCTTTGT GGACCCCAGT 
GGCCAGTTCG TGGACACTGT CAGGGTCCTG CCTTTTGACC CTCGAGGTGC TGGCGTGTAC 
CATGAGGTCA AGGCCATGCG AAAGAAAGCC CCCATCATTT TAGACGCCCG CCAGAGCAAC 
ACCATATCCC TCGGGGAAAT GGGAGAGGAG GCCCCCTTAG GAGAGCTGAT CATCCCGCCC 
GGGGCCTTCC GCAGAGCTGA CGGTCAACCC TACATGGGGG CTGTGGAGGC CCAGGTGACC 
TTCGTGGACC CCCGAAATCT CACTTCGGCA GCCGCCGCGC CCAGCGACCT GCGGTTCGTG 
GACGGGGACG GGGAGCTGGC CCCGCTGCGC ACGTACGGCA TGTTCTCGGT GGACCTTCGG 
GCCGCCGGCT CCGCCGAGCA GCTGCAGACG GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC 
CAGATCCGGA TGCCGGGCCA CGTGGAGGCC CTGAAGCTGT GGTCGCTGAA CCCTGACACG 
GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC 
CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC GTGGAGATCC GCGAGCGCCG TCTGTTCAAC 
CTGGACGTGC CCGAGCGCCG CCGCTGCTTC GTGAAGGTGC GCGCCTATGC CAACGACAAG 
TTCACCCCCA GCGAGCAGGT GGAGGGCGTG GTGGTCACGC TGGTCAATCT GGAGCCCGCC 
CCCGGCTTCT CTGCCAACCC TCGCGCCTGG GGCCGCTTCG ACAGCGCGGT CACCGGCCCG 
AACGGCGCCT GCCTCCCCGC CTTCTGCGAC GCCGACCAGC CCGACGCCTA CACCGCCGTG 
GTCACGGCCA CCCTGGGCGG GGAGGAGCTG GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC 
CCGGCCACCG TTGGCGTGGC GCAGCCCTAC CTGGACCGGC TGGCCTACCG CCGCACGGAC 
CATGACGACC CTGCCCTCAA GCGCAGTGGC TTCCGCATCA ACCTCGCCAA GCCCAGGCCC 
GGCGAACCCA CCGAGGCCAA CGGGCCCGTG TACCCGTGGC GCAGCCTGCG GGAGTGCCAG 
GAGGCCCCGG TGACCGCCAG TCACTTCCGC TTCGCTCGTG TGGAGGCGGA CAAATACGAG 
TACAACGTGG TCCCCTTCCG CGAGGGTGCG CCAGCCTCCT GGACCGGAGA TCTCCTGGCC 
TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC TGCTTCCTCA AGGTGAAGAT CCAAGGACCC 
CAGGAGTACA TGGTCCGCTC CCACAATTTG GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG 
CTCTACGGGC TGCGGGATTC CCGGAGTGTC CGGGACCCCC AGAGCCCTAG CTCCTCTGCC 
GCCTGTGTGG AATTCAAGTG CAGCGGGATG CTGTTTGACC AGCGTCAGGT GGACAGGACG 
CTGGTGACCA TCATGCCGCA AGGCAGCTGC CGGCGTGTGG CGGTCAACGG GCTCCTGCAG 
GATTACCTGA CACGGCACCC CCCACCCGCG CCCGCTGATG ACCTGGCTTC CTTCTCCATG 
CTGGCCCCGC TGGACCCTCT GGGTCACAAC TATGGTGTCT ACACGGTCAC CGACCAGAGC 
CCAAGGCTGG CCAAGGAGAT CGCCATTGGC CGCTGCTTCG ACGGCTCCTC CGACGGCTTC 
TCCAGGGAGA TGAAGGCTGA CGCGGGCACA GCCGTCACCT TCCAGTGCCG GGAGCCAACT 
GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG CTGGAGTCCC CATCGGCGGC TCTTGGGGAC 
ATCCGCAGGG AGATGGGCGA GGTGGCGCGC GTACAGGCCC GAGCCTCGAG TCCACTCCGC 
ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP2 ( XM_006080368.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080430.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu33260
Clone ID Related Accession (Same CDS sequence)	XM_006080368.2 , XM_006080368.1
Accession Version	XM_006080368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006080368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCAT CGCTGTCCCT CCAGACTTTG TTTCCCTGCC AGTATTCGGA ACCTTGGGAT 
TCCCGCCATC CCATGGGCAG CTCCACTCCT GGCACAAAGG GGTTAAGTCC ACTTGCACTT 
TACAGCCAAT GGCCATGCGC AGCGCCTTCC CGGCGTCTCC TGATTGGCGG ACACACAGAG 
TGGGCGGGAG GCGAGGGGAC GCCCCCTGTG CCCAAGGATG CCTTTAAAAG GCTGGGGCAG 
TGGTGGCTGC CTGCGTGCAC ACAGATCGCC TCCGCCATGA GCAGCACGGA TGGCCCGGAC 
CTAGCGGAGG CGCCCGAAGA GCGGTGTTTC CTCAGCACCG CGGAGGCAGC GGCCCTGGAG 
CGGGAGCTGC TGGAGGATTA TCGCTTTGGG CGGCAGCAGC TGGTGGAGTG GTGTGGCCAT 
GCTAGTGCCG TGGCTGTGAC CAAGGTGTTC CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG 
ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG AATGGAGCGG TGGGGCTGGC GTGTGCCCGG 
CACCTGCGCG TGTTCGAGTA CGAACCCACC ATCTTCTACC CCACGCGCTC GCCGGACCCA 
CTGCACCGGG ACCTGACTAT TCAGTGTGAG AAGATGGACA TCCCCTTCCT GTCCTATCTG 
CCCACAGAGG TCCAGCTCAT CAACAACGCC TACAGGCTAG TGGTGGACGC TGTGCTGGGC 
CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT CCCTGCACAC GCGCGCTGGC CACGCTCAAG 
CTACTGTCCA TCCCCCTCGT GAGCCTGGAC ATCCCCTCAG GCTGGGACCC AGAGACTGGC 
GGCGACGCCG AGGACGGGCT ACGACCCGAC GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC 
TGCGCCGGCC GCTTCTCTGG GCGCCACCAC TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG 
CACTTCCCGA CGTGGAGAAG GGAGGGTGGG CTCAGGAAGG GACTTCCAGT CGGCGACGAA 
GGGACGCAGG CCAGCCCGAG GGCGGAGGTA CAGCCGGATG CACCGAGGGG GTCGCCCCAG 
AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC GGGAGTTGCC CAGACGGGGC GTGGCCTGCA 
TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC CCATCCTCAG ACCCAGCGGC CGCCAGACCC 
GCGGGAGTCC GACCCGAGTG CGCAGCGGAG CTGGGACCCT CCCTCCGACC TTCGGACCTG 
GCCATGGCGT CACTGCCGAC ACTGCTCTGC CTCTGTGTTG CCGCCGCACA CCTGGCGGGG 
GCCCGAGGTA CTCCGGACGC CGAGGAGCCC ACGGTGACCG CGTGGGGCCT TGAAGGTTCA 
TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC CTGGAGGACT GGGAAGAGGC CAGCGAGTGG 
ACGTCCTGGT TCAACGTCGA CCACCCGGGC GGCGACGGTG ACTTCGAGAG CCTGGCCGCG 
ATCCGCTTCT ATTACGGGCC GGCACGCGTG TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC 
ACCACAGACT GGGCGCTGCC TTCGGATGTC GGCGAGCGCG TACACCTGAA CCCCACGCGC 
GGTTTCTGGT GCCTCAACCG CGAGCAGCCC CGAGGCCGCA GATGCTCCAA TTACCACGTG 
CGCTTCCGCT GCCCGCTCGA GGCCACGTGG GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG 
GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT 
GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT 
GGCCCCAACA CCTGTGGGTG CCCCGACCAC ATTCTTCTGG GCTCGGTGGT CACCCCATCT 
GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC TTGAGAGACT GGCCTGGCAC CATAGCCACT 
AGTGATGCCC ATGGAACCTT CCGGATGCCT GGAGTCTGTG CCAGCAGCCG AGCCAACGTC 
AGTGCCCAAA TGGACGGCTT TTCAGCAGGC ATGGCCCAGA CCCAGGCCAA CAGCTCCATG 
TCAGCAGTGG CCACTGTTGT CCTTAAAAAG TTGGAGAAGC CCTACCTGGT GAAGCAACCC 
GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT GTGACCTTCT GCTGCAAAGC CTCAGGCACC 
CCCATGCCCA AGAAATACTC CTGGTTCCAC AACGGGACGC TGCTGGACAG GCGGACACAC 
AGGTATGGGG CCCACCTGGA GCTGCATGGG CTCCGCCCAG ACCAAGCAGG CACCTATCAC 
TGCAAAGCGT GGAATGACGC GGGGGCCGTG CGATCGGGCA CTGCCCGACT CACCGTACTT 
GCCCCGGGCC AGCCAGCCTG TGACCCCCGG CCCCGAGAAC ACCTGATCAA GCTTCCAGAT 
GACTGTGGTC AGCCAGGCAG AGGGCCCACC TACCTGGACG TAGGCCTTTG CCCTGACACC 
CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC 
TCCGTGCGCC GCCTGGAGAG CAGGAAGATC CACTGCTCTG GCTACACCCT CCCAGTCAAG 
ATCGTGGCTG AGTGTGGCTG TCAGAAATGT CTGCCCCTTC GGGGGCTGAT CCGGGGACGC 
GTGATGGCCG CTGACTCTAG GGAGCCCTTG CGCTTTGCTC GCATCCTGCT GGGCCGGGAG 
CCCATTGGCT TCACGTCCTA CCAGGGTGAC TTCACCCTCG AGGTGGCGCC TTCCACTCAG 
CGGCTGGTGA TGACCTTTGT GGACCCCAGT GGCCAGTTCG TGGACACTGT CAGGGTCCTG 
CCTTTTGACC CTCGAGGTGC TGGCGTGTAC CATGAGGTCA AGGCCATGCG AAAGAAAGCC 
CCCATCATTT TAGACGCCCG CCAGAGCAAC ACCATATCCC TCGGGGAAAT GGGAGAGGAG 
GCCCCCTTAG GAGAGCTGAT CATCCCGCCC GGGGCCTTCC GCAGAGCTGA CGGTCAACCC 
TACATGGGGG CTGTGGAGGC CCAGGTGACC TTCGTGGACC CCCGAAATCT CACTTCGGCA 
GCCGCCGCGC CCAGCGACCT GCGGTTCGTG GACGGGGACG GGGAGCTGGC CCCGCTGCGC 
ACGTACGGCA TGTTCTCGGT GGACCTTCGG GCCGCCGGCT CCGCCGAGCA GCTGCAGACG 
GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC CAGATCCGGA TGCCGGGCCA CGTGGAGGCC 
CTGAAGCTGT GGTCGCTGAA CCCTGACACG GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG 
CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC 
GTGGAGATCC GCGAGCGCCG TCTGTTCAAC CTGGACGTGC CCGAGCGCCG CCGCTGCTTC 
GTGAAGGTGC GCGCCTATGC CAACGACAAG TTCACCCCCA GCGAGCAGGT GGAGGGCGTG 
GTGGTCACGC TGGTCAATCT GGAGCCCGCC CCCGGCTTCT CTGCCAACCC TCGCGCCTGG 
GGCCGCTTCG ACAGCGCGGT CACCGGCCCG AACGGCGCCT GCCTCCCCGC CTTCTGCGAC 
GCCGACCAGC CCGACGCCTA CACCGCCGTG GTCACGGCCA CCCTGGGCGG GGAGGAGCTG 
GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC CCGGCCACCG TTGGCGTGGC GCAGCCCTAC 
CTGGACCGGC TGGCCTACCG CCGCACGGAC CATGACGACC CTGCCCTCAA GCGCAGTGGC 
TTCCGCATCA ACCTCGCCAA GCCCAGGCCC GGCGAACCCA CCGAGGCCAA CGGGCCCGTG 
TACCCGTGGC GCAGCCTGCG GGAGTGCCAG GAGGCCCCGG TGACCGCCAG TCACTTCCGC 
TTCGCTCGTG TGGAGGCGGA CAAATACGAG TACAACGTGG TCCCCTTCCG CGAGGGTGCG 
CCAGCCTCCT GGACCGGAGA TCTCCTGGCC TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC 
TGCTTCCTCA AGGTGAAGAT CCAAGGACCC CAGGAGTACA TGGTCCGCTC CCACAATTTG 
GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG CTCTACGGGC TGCGGGATTC CCGGAGTGTC 
CGGGACCCCC AGAGCCCTAG CTCCTCTGCC GCCTGTGTGG AATTCAAGTG CAGCGGGATG 
CTGTTTGACC AGCGTCAGGT GGACAGGACG CTGGTGACCA TCATGCCGCA AGGCAGCTGC 
CGGCGTGTGG CGGTCAACGG GCTCCTGCAG GATTACCTGA CACGGCACCC CCCACCCGCG 
CCCGCTGATG ACCTGGCTTC CTTCTCCATG CTGGCCCCGC TGGACCCTCT GGGTCACAAC 
TATGGTGTCT ACACGGTCAC CGACCAGAGC CCAAGGCTGG CCAAGGAGAT CGCCATTGGC 
CGCTGCTTCG ACGGCTCCTC CGACGGCTTC TCCAGGGAGA TGAAGGCTGA CGCGGGCACA 
GCCGTCACCT TCCAGTGCCG GGAGCCAACT GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG 
CTGGAGTCCC CATCGGCGGC TCTTGGGGAC ATCCGCAGGG AGATGGGCGA GGTGGCGCGC 
GTACAGGCCC GAGCCTCGAG TCCACTCCGC ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080430.1
CDS	258..4994
Translation	MSPSLSLQTLFPCQYSEPWDSRHPMGSSTPGTKGLSPLALYSQWPCAAPSRRLLIGGHTEWAGGEGTPPVPKDAFKRLGQWWLPACTQIASAMSSTDGPDLAEAPEERCFLSTAEAAALERELLEDYRFGRQQLVEWCGHASAVAVTKVFPLPALPRKQRTALVVCGPEQNGAVGLACARHLRVFEYEPTIFYPTRSPDPLHRDLTIQCEKMDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_006080368.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080368.2

ORF Insert Sequence:

ATGAGCCCAT CGCTGTCCCT CCAGACTTTG TTTCCCTGCC AGTATTCGGA ACCTTGGGAT 
TCCCGCCATC CCATGGGCAG CTCCACTCCT GGCACAAAGG GGTTAAGTCC ACTTGCACTT 
TACAGCCAAT GGCCATGCGC AGCGCCTTCC CGGCGTCTCC TGATTGGCGG ACACACAGAG 
TGGGCGGGAG GCGAGGGGAC GCCCCCTGTG CCCAAGGATG CCTTTAAAAG GCTGGGGCAG 
TGGTGGCTGC CTGCGTGCAC ACAGATCGCC TCCGCCATGA GCAGCACGGA TGGCCCGGAC 
CTAGCGGAGG CGCCCGAAGA GCGGTGTTTC CTCAGCACCG CGGAGGCAGC GGCCCTGGAG 
CGGGAGCTGC TGGAGGATTA TCGCTTTGGG CGGCAGCAGC TGGTGGAGTG GTGTGGCCAT 
GCTAGTGCCG TGGCTGTGAC CAAGGTGTTC CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG 
ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG AATGGAGCGG TGGGGCTGGC GTGTGCCCGG 
CACCTGCGCG TGTTCGAGTA CGAACCCACC ATCTTCTACC CCACGCGCTC GCCGGACCCA 
CTGCACCGGG ACCTGACTAT TCAGTGTGAG AAGATGGACA TCCCCTTCCT GTCCTATCTG 
CCCACAGAGG TCCAGCTCAT CAACAACGCC TACAGGCTAG TGGTGGACGC TGTGCTGGGC 
CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT CCCTGCACAC GCGCGCTGGC CACGCTCAAG 
CTACTGTCCA TCCCCCTCGT GAGCCTGGAC ATCCCCTCAG GCTGGGACCC AGAGACTGGC 
GGCGACGCCG AGGACGGGCT ACGACCCGAC GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC 
TGCGCCGGCC GCTTCTCTGG GCGCCACCAC TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG 
CACTTCCCGA CGTGGAGAAG GGAGGGTGGG CTCAGGAAGG GACTTCCAGT CGGCGACGAA 
GGGACGCAGG CCAGCCCGAG GGCGGAGGTA CAGCCGGATG CACCGAGGGG GTCGCCCCAG 
AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC GGGAGTTGCC CAGACGGGGC GTGGCCTGCA 
TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC CCATCCTCAG ACCCAGCGGC CGCCAGACCC 
GCGGGAGTCC GACCCGAGTG CGCAGCGGAG CTGGGACCCT CCCTCCGACC TTCGGACCTG 
GCCATGGCGT CACTGCCGAC ACTGCTCTGC CTCTGTGTTG CCGCCGCACA CCTGGCGGGG 
GCCCGAGGTA CTCCGGACGC CGAGGAGCCC ACGGTGACCG CGTGGGGCCT TGAAGGTTCA 
TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC CTGGAGGACT GGGAAGAGGC CAGCGAGTGG 
ACGTCCTGGT TCAACGTCGA CCACCCGGGC GGCGACGGTG ACTTCGAGAG CCTGGCCGCG 
ATCCGCTTCT ATTACGGGCC GGCACGCGTG TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC 
ACCACAGACT GGGCGCTGCC TTCGGATGTC GGCGAGCGCG TACACCTGAA CCCCACGCGC 
GGTTTCTGGT GCCTCAACCG CGAGCAGCCC CGAGGCCGCA GATGCTCCAA TTACCACGTG 
CGCTTCCGCT GCCCGCTCGA GGCCACGTGG GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG 
GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT 
GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT 
GGCCCCAACA CCTGTGGGTG CCCCGACCAC ATTCTTCTGG GCTCGGTGGT CACCCCATCT 
GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC TTGAGAGACT GGCCTGGCAC CATAGCCACT 
AGTGATGCCC ATGGAACCTT CCGGATGCCT GGAGTCTGTG CCAGCAGCCG AGCCAACGTC 
AGTGCCCAAA TGGACGGCTT TTCAGCAGGC ATGGCCCAGA CCCAGGCCAA CAGCTCCATG 
TCAGCAGTGG CCACTGTTGT CCTTAAAAAG TTGGAGAAGC CCTACCTGGT GAAGCAACCC 
GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT GTGACCTTCT GCTGCAAAGC CTCAGGCACC 
CCCATGCCCA AGAAATACTC CTGGTTCCAC AACGGGACGC TGCTGGACAG GCGGACACAC 
AGGTATGGGG CCCACCTGGA GCTGCATGGG CTCCGCCCAG ACCAAGCAGG CACCTATCAC 
TGCAAAGCGT GGAATGACGC GGGGGCCGTG CGATCGGGCA CTGCCCGACT CACCGTACTT 
GCCCCGGGCC AGCCAGCCTG TGACCCCCGG CCCCGAGAAC ACCTGATCAA GCTTCCAGAT 
GACTGTGGTC AGCCAGGCAG AGGGCCCACC TACCTGGACG TAGGCCTTTG CCCTGACACC 
CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC 
TCCGTGCGCC GCCTGGAGAG CAGGAAGATC CACTGCTCTG GCTACACCCT CCCAGTCAAG 
ATCGTGGCTG AGTGTGGCTG TCAGAAATGT CTGCCCCTTC GGGGGCTGAT CCGGGGACGC 
GTGATGGCCG CTGACTCTAG GGAGCCCTTG CGCTTTGCTC GCATCCTGCT GGGCCGGGAG 
CCCATTGGCT TCACGTCCTA CCAGGGTGAC TTCACCCTCG AGGTGGCGCC TTCCACTCAG 
CGGCTGGTGA TGACCTTTGT GGACCCCAGT GGCCAGTTCG TGGACACTGT CAGGGTCCTG 
CCTTTTGACC CTCGAGGTGC TGGCGTGTAC CATGAGGTCA AGGCCATGCG AAAGAAAGCC 
CCCATCATTT TAGACGCCCG CCAGAGCAAC ACCATATCCC TCGGGGAAAT GGGAGAGGAG 
GCCCCCTTAG GAGAGCTGAT CATCCCGCCC GGGGCCTTCC GCAGAGCTGA CGGTCAACCC 
TACATGGGGG CTGTGGAGGC CCAGGTGACC TTCGTGGACC CCCGAAATCT CACTTCGGCA 
GCCGCCGCGC CCAGCGACCT GCGGTTCGTG GACGGGGACG GGGAGCTGGC CCCGCTGCGC 
ACGTACGGCA TGTTCTCGGT GGACCTTCGG GCCGCCGGCT CCGCCGAGCA GCTGCAGACG 
GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC CAGATCCGGA TGCCGGGCCA CGTGGAGGCC 
CTGAAGCTGT GGTCGCTGAA CCCTGACACG GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG 
CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC 
GTGGAGATCC GCGAGCGCCG TCTGTTCAAC CTGGACGTGC CCGAGCGCCG CCGCTGCTTC 
GTGAAGGTGC GCGCCTATGC CAACGACAAG TTCACCCCCA GCGAGCAGGT GGAGGGCGTG 
GTGGTCACGC TGGTCAATCT GGAGCCCGCC CCCGGCTTCT CTGCCAACCC TCGCGCCTGG 
GGCCGCTTCG ACAGCGCGGT CACCGGCCCG AACGGCGCCT GCCTCCCCGC CTTCTGCGAC 
GCCGACCAGC CCGACGCCTA CACCGCCGTG GTCACGGCCA CCCTGGGCGG GGAGGAGCTG 
GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC CCGGCCACCG TTGGCGTGGC GCAGCCCTAC 
CTGGACCGGC TGGCCTACCG CCGCACGGAC CATGACGACC CTGCCCTCAA GCGCAGTGGC 
TTCCGCATCA ACCTCGCCAA GCCCAGGCCC GGCGAACCCA CCGAGGCCAA CGGGCCCGTG 
TACCCGTGGC GCAGCCTGCG GGAGTGCCAG GAGGCCCCGG TGACCGCCAG TCACTTCCGC 
TTCGCTCGTG TGGAGGCGGA CAAATACGAG TACAACGTGG TCCCCTTCCG CGAGGGTGCG 
CCAGCCTCCT GGACCGGAGA TCTCCTGGCC TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC 
TGCTTCCTCA AGGTGAAGAT CCAAGGACCC CAGGAGTACA TGGTCCGCTC CCACAATTTG 
GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG CTCTACGGGC TGCGGGATTC CCGGAGTGTC 
CGGGACCCCC AGAGCCCTAG CTCCTCTGCC GCCTGTGTGG AATTCAAGTG CAGCGGGATG 
CTGTTTGACC AGCGTCAGGT GGACAGGACG CTGGTGACCA TCATGCCGCA AGGCAGCTGC 
CGGCGTGTGG CGGTCAACGG GCTCCTGCAG GATTACCTGA CACGGCACCC CCCACCCGCG 
CCCGCTGATG ACCTGGCTTC CTTCTCCATG CTGGCCCCGC TGGACCCTCT GGGTCACAAC 
TATGGTGTCT ACACGGTCAC CGACCAGAGC CCAAGGCTGG CCAAGGAGAT CGCCATTGGC 
CGCTGCTTCG ACGGCTCCTC CGACGGCTTC TCCAGGGAGA TGAAGGCTGA CGCGGGCACA 
GCCGTCACCT TCCAGTGCCG GGAGCCAACT GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG 
CTGGAGTCCC CATCGGCGGC TCTTGGGGAC ATCCGCAGGG AGATGGGCGA GGTGGCGCGC 
GTACAGGCCC GAGCCTCGAG TCCACTCCGC ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CILP2 ( XM_006080368.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006080430.1 Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), mRNA.

Price & Availability↑

CloneID	OBu33260
Clone ID Related Accession (Same CDS sequence)	XM_006080368.2 , XM_006080368.1
Accession Version	XM_006080368.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cartilage intermediate layer protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785887.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCAT CGCTGTCCCT CCAGACTTTG TTTCCCTGCC AGTATTCGGA ACCTTGGGAT 
TCCCGCCATC CCATGGGCAG CTCCACTCCT GGCACAAAGG GGTTAAGTCC ACTTGCACTT 
TACAGCCAAT GGCCATGCGC AGCGCCTTCC CGGCGTCTCC TGATTGGCGG ACACACAGAG 
TGGGCGGGAG GCGAGGGGAC GCCCCCTGTG CCCAAGGATG CCTTTAAAAG GCTGGGGCAG 
TGGTGGCTGC CTGCGTGCAC ACAGATCGCC TCCGCCATGA GCAGCACGGA TGGCCCGGAC 
CTAGCGGAGG CGCCCGAAGA GCGGTGTTTC CTCAGCACCG CGGAGGCAGC GGCCCTGGAG 
CGGGAGCTGC TGGAGGATTA TCGCTTTGGG CGGCAGCAGC TGGTGGAGTG GTGTGGCCAT 
GCTAGTGCCG TGGCTGTGAC CAAGGTGTTC CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG 
ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG AATGGAGCGG TGGGGCTGGC GTGTGCCCGG 
CACCTGCGCG TGTTCGAGTA CGAACCCACC ATCTTCTACC CCACGCGCTC GCCGGACCCA 
CTGCACCGGG ACCTGACTAT TCAGTGTGAG AAGATGGACA TCCCCTTCCT GTCCTATCTG 
CCCACAGAGG TCCAGCTCAT CAACAACGCC TACAGGCTAG TGGTGGACGC TGTGCTGGGC 
CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT CCCTGCACAC GCGCGCTGGC CACGCTCAAG 
CTACTGTCCA TCCCCCTCGT GAGCCTGGAC ATCCCCTCAG GCTGGGACCC AGAGACTGGC 
GGCGACGCCG AGGACGGGCT ACGACCCGAC GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC 
TGCGCCGGCC GCTTCTCTGG GCGCCACCAC TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG 
CACTTCCCGA CGTGGAGAAG GGAGGGTGGG CTCAGGAAGG GACTTCCAGT CGGCGACGAA 
GGGACGCAGG CCAGCCCGAG GGCGGAGGTA CAGCCGGATG CACCGAGGGG GTCGCCCCAG 
AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC GGGAGTTGCC CAGACGGGGC GTGGCCTGCA 
TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC CCATCCTCAG ACCCAGCGGC CGCCAGACCC 
GCGGGAGTCC GACCCGAGTG CGCAGCGGAG CTGGGACCCT CCCTCCGACC TTCGGACCTG 
GCCATGGCGT CACTGCCGAC ACTGCTCTGC CTCTGTGTTG CCGCCGCACA CCTGGCGGGG 
GCCCGAGGTA CTCCGGACGC CGAGGAGCCC ACGGTGACCG CGTGGGGCCT TGAAGGTTCA 
TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC CTGGAGGACT GGGAAGAGGC CAGCGAGTGG 
ACGTCCTGGT TCAACGTCGA CCACCCGGGC GGCGACGGTG ACTTCGAGAG CCTGGCCGCG 
ATCCGCTTCT ATTACGGGCC GGCACGCGTG TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC 
ACCACAGACT GGGCGCTGCC TTCGGATGTC GGCGAGCGCG TACACCTGAA CCCCACGCGC 
GGTTTCTGGT GCCTCAACCG CGAGCAGCCC CGAGGCCGCA GATGCTCCAA TTACCACGTG 
CGCTTCCGCT GCCCGCTCGA GGCCACGTGG GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG 
GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT 
GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT 
GGCCCCAACA CCTGTGGGTG CCCCGACCAC ATTCTTCTGG GCTCGGTGGT CACCCCATCT 
GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC TTGAGAGACT GGCCTGGCAC CATAGCCACT 
AGTGATGCCC ATGGAACCTT CCGGATGCCT GGAGTCTGTG CCAGCAGCCG AGCCAACGTC 
AGTGCCCAAA TGGACGGCTT TTCAGCAGGC ATGGCCCAGA CCCAGGCCAA CAGCTCCATG 
TCAGCAGTGG CCACTGTTGT CCTTAAAAAG TTGGAGAAGC CCTACCTGGT GAAGCAACCC 
GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT GTGACCTTCT GCTGCAAAGC CTCAGGCACC 
CCCATGCCCA AGAAATACTC CTGGTTCCAC AACGGGACGC TGCTGGACAG GCGGACACAC 
AGGTATGGGG CCCACCTGGA GCTGCATGGG CTCCGCCCAG ACCAAGCAGG CACCTATCAC 
TGCAAAGCGT GGAATGACGC GGGGGCCGTG CGATCGGGCA CTGCCCGACT CACCGTACTT 
GCCCCGGGCC AGCCAGCCTG TGACCCCCGG CCCCGAGAAC ACCTGATCAA GCTTCCAGAT 
GACTGTGGTC AGCCAGGCAG AGGGCCCACC TACCTGGACG TAGGCCTTTG CCCTGACACC 
CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC 
TCCGTGCGCC GCCTGGAGAG CAGGAAGATC CACTGCTCTG GCTACACCCT CCCAGTCAAG 
ATCGTGGCTG AGTGTGGCTG TCAGAAATGT CTGCCCCTTC GGGGGCTGAT CCGGGGACGC 
GTGATGGCCG CTGACTCTAG GGAGCCCTTG CGCTTTGCTC GCATCCTGCT GGGCCGGGAG 
CCCATTGGCT TCACGTCCTA CCAGGGTGAC TTCACCCTCG AGGTGGCGCC TTCCACTCAG 
CGGCTGGTGA TGACCTTTGT GGACCCCAGT GGCCAGTTCG TGGACACTGT CAGGGTCCTG 
CCTTTTGACC CTCGAGGTGC TGGCGTGTAC CATGAGGTCA AGGCCATGCG AAAGAAAGCC 
CCCATCATTT TAGACGCCCG CCAGAGCAAC ACCATATCCC TCGGGGAAAT GGGAGAGGAG 
GCCCCCTTAG GAGAGCTGAT CATCCCGCCC GGGGCCTTCC GCAGAGCTGA CGGTCAACCC 
TACATGGGGG CTGTGGAGGC CCAGGTGACC TTCGTGGACC CCCGAAATCT CACTTCGGCA 
GCCGCCGCGC CCAGCGACCT GCGGTTCGTG GACGGGGACG GGGAGCTGGC CCCGCTGCGC 
ACGTACGGCA TGTTCTCGGT GGACCTTCGG GCCGCCGGCT CCGCCGAGCA GCTGCAGACG 
GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC CAGATCCGGA TGCCGGGCCA CGTGGAGGCC 
CTGAAGCTGT GGTCGCTGAA CCCTGACACG GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG 
CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC 
GTGGAGATCC GCGAGCGCCG TCTGTTCAAC CTGGACGTGC CCGAGCGCCG CCGCTGCTTC 
GTGAAGGTGC GCGCCTATGC CAACGACAAG TTCACCCCCA GCGAGCAGGT GGAGGGCGTG 
GTGGTCACGC TGGTCAATCT GGAGCCCGCC CCCGGCTTCT CTGCCAACCC TCGCGCCTGG 
GGCCGCTTCG ACAGCGCGGT CACCGGCCCG AACGGCGCCT GCCTCCCCGC CTTCTGCGAC 
GCCGACCAGC CCGACGCCTA CACCGCCGTG GTCACGGCCA CCCTGGGCGG GGAGGAGCTG 
GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC CCGGCCACCG TTGGCGTGGC GCAGCCCTAC 
CTGGACCGGC TGGCCTACCG CCGCACGGAC CATGACGACC CTGCCCTCAA GCGCAGTGGC 
TTCCGCATCA ACCTCGCCAA GCCCAGGCCC GGCGAACCCA CCGAGGCCAA CGGGCCCGTG 
TACCCGTGGC GCAGCCTGCG GGAGTGCCAG GAGGCCCCGG TGACCGCCAG TCACTTCCGC 
TTCGCTCGTG TGGAGGCGGA CAAATACGAG TACAACGTGG TCCCCTTCCG CGAGGGTGCG 
CCAGCCTCCT GGACCGGAGA TCTCCTGGCC TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC 
TGCTTCCTCA AGGTGAAGAT CCAAGGACCC CAGGAGTACA TGGTCCGCTC CCACAATTTG 
GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG CTCTACGGGC TGCGGGATTC CCGGAGTGTC 
CGGGACCCCC AGAGCCCTAG CTCCTCTGCC GCCTGTGTGG AATTCAAGTG CAGCGGGATG 
CTGTTTGACC AGCGTCAGGT GGACAGGACG CTGGTGACCA TCATGCCGCA AGGCAGCTGC 
CGGCGTGTGG CGGTCAACGG GCTCCTGCAG GATTACCTGA CACGGCACCC CCCACCCGCG 
CCCGCTGATG ACCTGGCTTC CTTCTCCATG CTGGCCCCGC TGGACCCTCT GGGTCACAAC 
TATGGTGTCT ACACGGTCAC CGACCAGAGC CCAAGGCTGG CCAAGGAGAT CGCCATTGGC 
CGCTGCTTCG ACGGCTCCTC CGACGGCTTC TCCAGGGAGA TGAAGGCTGA CGCGGGCACA 
GCCGTCACCT TCCAGTGCCG GGAGCCAACT GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG 
CTGGAGTCCC CATCGGCGGC TCTTGGGGAC ATCCGCAGGG AGATGGGCGA GGTGGCGCGC 
GTACAGGCCC GAGCCTCGAG TCCACTCCGC ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006080430.1
CDS	239..4975
Translation	MSPSLSLQTLFPCQYSEPWDSRHPMGSSTPGTKGLSPLALYSQWPCAAPSRRLLIGGHTEWAGGEGTPPVPKDAFKRLGQWWLPACTQIASAMSSTDGPDLAEAPEERCFLSTAEAAALERELLEDYRFGRQQLVEWCGHASAVAVTKVFPLPALPRKQRTALVVCGPEQNGAVGLACARHLRVFEYEPTIFYPTRSPDPLHRDLTIQCEKMDIPFLSYLPTEVQLINNAYRLVVDAVLGPGVEPAEVGGPCTRALATLKLLSIPLVSLDIPSGWDPETGGDAEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRLCSRRHFPTWRREGGLRKGLPVGDEGTQASPRAEVQPDAPRGSPQRGGHPSHLGPGSCPDGAWPAWGVACGRVPPPSSDPAAARPAGVRPECAAELGPSLRPSDLAMASLPTLLCLCVAAAHLAGARGTPDAEEPTVTAWGLEGSSLRPGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPSDVGERVHLNPTRGFWCLNREQPRGRRCSNYHVRFRCPLEATWGSWGPWGPCSGSCGPGRRLRRRRCSSPAGDACPGRPSEAQKCVRPRCPGCGPNTCGCPDHILLGSVVTPSGRPLPGARVSLRDWPGTIATSDAHGTFRMPGVCASSRANVSAQMDGFSAGMAQTQANSSMSAVATVVLKKLEKPYLVKQPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRTHRYGAHLELHGLRPDQAGTYHCKAWNDAGAVRSGTARLTVLAPGQPACDPRPREHLIKLPDDCGQPGRGPTYLDVGLCPDTLCPSPAGSRPRCGDAGSRCCSVRRLESRKIHCSGYTLPVKIVAECGCQKCLPLRGLIRGRVMAADSREPLRFARILLGREPIGFTSYQGDFTLEVAPSTQRLVMTFVDPSGQFVDTVRVLPFDPRGAGVYHEVKAMRKKAPIILDARQSNTISLGEMGEEAPLGELIIPPGAFRRADGQPYMGAVEAQVTFVDPRNLTSAAAAPSDLRFVDGDGELAPLRTYGMFSVDLRAAGSAEQLQTGLVAVRVAADQIRMPGHVEALKLWSLNPDTGLWEEESGFQRERPASQRARREERVFLVGNVEIRERRLFNLDVPERRRCFVKVRAYANDKFTPSEQVEGVVVTLVNLEPAPGFSANPRAWGRFDSAVTGPNGACLPAFCDADQPDAYTAVVTATLGGEELEPAPSRPRPLPATVGVAQPYLDRLAYRRTDHDDPALKRSGFRINLAKPRPGEPTEANGPVYPWRSLRECQEAPVTASHFRFARVEADKYEYNVVPFREGAPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNLGGSHPRTQGQLYGLRDSRSVRDPQSPSSSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLQDYLTRHPPPAPADDLASFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPTAGRPSLFQRLLESPSAALGDIRREMGEVARVQARASSPLRTRRGRAQQ

Target ORF information:

RefSeq Version	XM_006080368.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cartilage intermediate layer protein 2 (CILP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006080368.1

ORF Insert Sequence:

ATGAGCCCAT CGCTGTCCCT CCAGACTTTG TTTCCCTGCC AGTATTCGGA ACCTTGGGAT 
TCCCGCCATC CCATGGGCAG CTCCACTCCT GGCACAAAGG GGTTAAGTCC ACTTGCACTT 
TACAGCCAAT GGCCATGCGC AGCGCCTTCC CGGCGTCTCC TGATTGGCGG ACACACAGAG 
TGGGCGGGAG GCGAGGGGAC GCCCCCTGTG CCCAAGGATG CCTTTAAAAG GCTGGGGCAG 
TGGTGGCTGC CTGCGTGCAC ACAGATCGCC TCCGCCATGA GCAGCACGGA TGGCCCGGAC 
CTAGCGGAGG CGCCCGAAGA GCGGTGTTTC CTCAGCACCG CGGAGGCAGC GGCCCTGGAG 
CGGGAGCTGC TGGAGGATTA TCGCTTTGGG CGGCAGCAGC TGGTGGAGTG GTGTGGCCAT 
GCTAGTGCCG TGGCTGTGAC CAAGGTGTTC CCTCTGCCCG CTCTCCCCCG GAAGCAGAGG 
ACGGCGCTGG TCGTGTGCGG CCCAGAGCAG AATGGAGCGG TGGGGCTGGC GTGTGCCCGG 
CACCTGCGCG TGTTCGAGTA CGAACCCACC ATCTTCTACC CCACGCGCTC GCCGGACCCA 
CTGCACCGGG ACCTGACTAT TCAGTGTGAG AAGATGGACA TCCCCTTCCT GTCCTATCTG 
CCCACAGAGG TCCAGCTCAT CAACAACGCC TACAGGCTAG TGGTGGACGC TGTGCTGGGC 
CCTGGTGTGG AGCCGGCCGA GGTCGGGGGT CCCTGCACAC GCGCGCTGGC CACGCTCAAG 
CTACTGTCCA TCCCCCTCGT GAGCCTGGAC ATCCCCTCAG GCTGGGACCC AGAGACTGGC 
GGCGACGCCG AGGACGGGCT ACGACCCGAC GTGCTCGTAT CGCTGGCGGC GCCCAAGCGC 
TGCGCCGGCC GCTTCTCTGG GCGCCACCAC TTCGTGGCGG GCAGGCTCTG TTCCAGGAGG 
CACTTCCCGA CGTGGAGAAG GGAGGGTGGG CTCAGGAAGG GACTTCCAGT CGGCGACGAA 
GGGACGCAGG CCAGCCCGAG GGCGGAGGTA CAGCCGGATG CACCGAGGGG GTCGCCCCAG 
AGGGGTGGTC ACCCAAGTCA CCTCGGGCCC GGGAGTTGCC CAGACGGGGC GTGGCCTGCA 
TGGGGCGTGG CCTGCGGCCG CGTCCCTCCC CCATCCTCAG ACCCAGCGGC CGCCAGACCC 
GCGGGAGTCC GACCCGAGTG CGCAGCGGAG CTGGGACCCT CCCTCCGACC TTCGGACCTG 
GCCATGGCGT CACTGCCGAC ACTGCTCTGC CTCTGTGTTG CCGCCGCACA CCTGGCGGGG 
GCCCGAGGTA CTCCGGACGC CGAGGAGCCC ACGGTGACCG CGTGGGGCCT TGAAGGTTCA 
TCGTTGCGCC CTGGCCAGCC CTCGCCAGCC CTGGAGGACT GGGAAGAGGC CAGCGAGTGG 
ACGTCCTGGT TCAACGTCGA CCACCCGGGC GGCGACGGTG ACTTCGAGAG CCTGGCCGCG 
ATCCGCTTCT ATTACGGGCC GGCACGCGTG TGCCCAAGGC CGCTGGCGCT GGAGGCGCGC 
ACCACAGACT GGGCGCTGCC TTCGGATGTC GGCGAGCGCG TACACCTGAA CCCCACGCGC 
GGTTTCTGGT GCCTCAACCG CGAGCAGCCC CGAGGCCGCA GATGCTCCAA TTACCACGTG 
CGCTTCCGCT GCCCGCTCGA GGCCACGTGG GGTTCGTGGG GCCCTTGGGG TCCCTGTTCG 
GGGAGCTGCG GGCCAGGCCG TCGCTTGCGC CGCCGCCGCT GCTCAAGCCC CGCGGGGGAT 
GCGTGTCCCG GGCGTCCCTC GGAGGCGCAG AAGTGTGTGA GGCCTCGGTG TCCAGGGTGT 
GGCCCCAACA CCTGTGGGTG CCCCGACCAC ATTCTTCTGG GCTCGGTGGT CACCCCATCT 
GGGCGTCCAC TGCCAGGAGC CAGGGTCTCC TTGAGAGACT GGCCTGGCAC CATAGCCACT 
AGTGATGCCC ATGGAACCTT CCGGATGCCT GGAGTCTGTG CCAGCAGCCG AGCCAACGTC 
AGTGCCCAAA TGGACGGCTT TTCAGCAGGC ATGGCCCAGA CCCAGGCCAA CAGCTCCATG 
TCAGCAGTGG CCACTGTTGT CCTTAAAAAG TTGGAGAAGC CCTACCTGGT GAAGCAACCC 
GAGTCTCGAG TTCGAGAGGC TGGCCAGAAT GTGACCTTCT GCTGCAAAGC CTCAGGCACC 
CCCATGCCCA AGAAATACTC CTGGTTCCAC AACGGGACGC TGCTGGACAG GCGGACACAC 
AGGTATGGGG CCCACCTGGA GCTGCATGGG CTCCGCCCAG ACCAAGCAGG CACCTATCAC 
TGCAAAGCGT GGAATGACGC GGGGGCCGTG CGATCGGGCA CTGCCCGACT CACCGTACTT 
GCCCCGGGCC AGCCAGCCTG TGACCCCCGG CCCCGAGAAC ACCTGATCAA GCTTCCAGAT 
GACTGTGGTC AGCCAGGCAG AGGGCCCACC TACCTGGACG TAGGCCTTTG CCCTGACACC 
CTCTGCCCCA GCCCTGCAGG CTCCAGGCCC CGGTGTGGGG ACGCAGGCTC CCGCTGCTGC 
TCCGTGCGCC GCCTGGAGAG CAGGAAGATC CACTGCTCTG GCTACACCCT CCCAGTCAAG 
ATCGTGGCTG AGTGTGGCTG TCAGAAATGT CTGCCCCTTC GGGGGCTGAT CCGGGGACGC 
GTGATGGCCG CTGACTCTAG GGAGCCCTTG CGCTTTGCTC GCATCCTGCT GGGCCGGGAG 
CCCATTGGCT TCACGTCCTA CCAGGGTGAC TTCACCCTCG AGGTGGCGCC TTCCACTCAG 
CGGCTGGTGA TGACCTTTGT GGACCCCAGT GGCCAGTTCG TGGACACTGT CAGGGTCCTG 
CCTTTTGACC CTCGAGGTGC TGGCGTGTAC CATGAGGTCA AGGCCATGCG AAAGAAAGCC 
CCCATCATTT TAGACGCCCG CCAGAGCAAC ACCATATCCC TCGGGGAAAT GGGAGAGGAG 
GCCCCCTTAG GAGAGCTGAT CATCCCGCCC GGGGCCTTCC GCAGAGCTGA CGGTCAACCC 
TACATGGGGG CTGTGGAGGC CCAGGTGACC TTCGTGGACC CCCGAAATCT CACTTCGGCA 
GCCGCCGCGC CCAGCGACCT GCGGTTCGTG GACGGGGACG GGGAGCTGGC CCCGCTGCGC 
ACGTACGGCA TGTTCTCGGT GGACCTTCGG GCCGCCGGCT CCGCCGAGCA GCTGCAGACG 
GGGCTGGTGG CTGTGCGCGT GGCCGCTGAC CAGATCCGGA TGCCGGGCCA CGTGGAGGCC 
CTGAAGCTGT GGTCGCTGAA CCCTGACACG GGCTTGTGGG AAGAAGAAAG CGGCTTCCAG 
CGCGAGAGAC CGGCGTCCCA GCGGGCCCGC CGGGAGGAGC GGGTCTTCCT GGTGGGCAAC 
GTGGAGATCC GCGAGCGCCG TCTGTTCAAC CTGGACGTGC CCGAGCGCCG CCGCTGCTTC 
GTGAAGGTGC GCGCCTATGC CAACGACAAG TTCACCCCCA GCGAGCAGGT GGAGGGCGTG 
GTGGTCACGC TGGTCAATCT GGAGCCCGCC CCCGGCTTCT CTGCCAACCC TCGCGCCTGG 
GGCCGCTTCG ACAGCGCGGT CACCGGCCCG AACGGCGCCT GCCTCCCCGC CTTCTGCGAC 
GCCGACCAGC CCGACGCCTA CACCGCCGTG GTCACGGCCA CCCTGGGCGG GGAGGAGCTG 
GAGCCCGCGC CCTCGCGGCC GCGCCCGCTC CCGGCCACCG TTGGCGTGGC GCAGCCCTAC 
CTGGACCGGC TGGCCTACCG CCGCACGGAC CATGACGACC CTGCCCTCAA GCGCAGTGGC 
TTCCGCATCA ACCTCGCCAA GCCCAGGCCC GGCGAACCCA CCGAGGCCAA CGGGCCCGTG 
TACCCGTGGC GCAGCCTGCG GGAGTGCCAG GAGGCCCCGG TGACCGCCAG TCACTTCCGC 
TTCGCTCGTG TGGAGGCGGA CAAATACGAG TACAACGTGG TCCCCTTCCG CGAGGGTGCG 
CCAGCCTCCT GGACCGGAGA TCTCCTGGCC TGGTGGCCCA ACCCGCAGGA GTTCCGGGCC 
TGCTTCCTCA AGGTGAAGAT CCAAGGACCC CAGGAGTACA TGGTCCGCTC CCACAATTTG 
GGAGGCAGCC ATCCCCGGAC CCAGGGCCAG CTCTACGGGC TGCGGGATTC CCGGAGTGTC 
CGGGACCCCC AGAGCCCTAG CTCCTCTGCC GCCTGTGTGG AATTCAAGTG CAGCGGGATG 
CTGTTTGACC AGCGTCAGGT GGACAGGACG CTGGTGACCA TCATGCCGCA AGGCAGCTGC 
CGGCGTGTGG CGGTCAACGG GCTCCTGCAG GATTACCTGA CACGGCACCC CCCACCCGCG 
CCCGCTGATG ACCTGGCTTC CTTCTCCATG CTGGCCCCGC TGGACCCTCT GGGTCACAAC 
TATGGTGTCT ACACGGTCAC CGACCAGAGC CCAAGGCTGG CCAAGGAGAT CGCCATTGGC 
CGCTGCTTCG ACGGCTCCTC CGACGGCTTC TCCAGGGAGA TGAAGGCTGA CGCGGGCACA 
GCCGTCACCT TCCAGTGCCG GGAGCCAACT GCGGGCCGGC CCAGCCTCTT CCAGAGGCTG 
CTGGAGTCCC CATCGGCGGC TCTTGGGGAC ATCCGCAGGG AGATGGGCGA GGTGGCGCGC 
GTACAGGCCC GAGCCTCGAG TCCACTCCGC ACCCGCCGAG GCAGGGCCCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CILP2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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