CUX1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025275397.1	XP_025131182.1	protein CASP isoform X1
XM_025275400.1	XP_025131185.1	protein CASP isoform X4
XM_025275401.1	XP_025131186.1	protein CASP isoform X5
XM_025275402.1	XP_025131187.1	protein CASP isoform X6
XM_025275404.1	XP_025131189.1	protein CASP isoform X8
XM_025275406.1	XP_025131191.1	protein CASP isoform X9
XM_025275398.1	XP_025131183.1	protein CASP isoform X2
XM_006059466.1	XP_006059528.1	homeobox protein cut-like 1-like
XM_025275399.1	XP_025131184.1	protein CASP isoform X3
XM_025275403.1	XP_025131188.1	protein CASP isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu60248	XM_025275403.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu60249	XM_025275404.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu60250	XM_025275406.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu60247	XM_025275402.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu60242	XM_025275397.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu60243	XM_025275398.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu60244	XM_025275399.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu60245	XM_025275400.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu60246	XM_025275401.1 Latest version!	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu16058	XM_006059466.1 Latest version!	Bubalus bubalis cut-like homeobox 1 (CUX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

CUX1 ( XM_025275403.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131188.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu60248
Clone ID Related Accession (Same CDS sequence)	XM_025275403.1
Accession Version	XM_025275403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGCGCTGC GCGGAGCTGC AGGTCCACGT CACCGAGCTC 
ACGGCCACGG CAGCTGAGCA GAGGGAGCTG ATCGCCCGCT TGGAGCAGGA CCTCAGCACC 
ATCCAGTCCA TCCAGCGGCC AGACGCCGAG GGCGCAGCCG AGCTCGGCCT GGAGAAGATT 
CCCGAGCCCA TCAAGGAGGC CACCGCCCTG TTCTATGGAC CCTCAGCGCC TGCCAGCTCC 
CTCCCCGAGG GCCAGGTGGA CTCGCTGCTC TCCATCATCT CCAGCCAGAG GGAGCGCTTC 
CGGGCCCGGA ACCAGGAGCT GGAGGCGGAG AACCGCCTGG CCCAGCATAC CATCCAAGCC 
CTGCAGACCG AGCTGGACAG CCTGCGCGCG GACAACATCA AGCTCTTTGA GAAGATCAAG 
TTCCTGCAGA GCTACCCTGG CCGGGGCGGC GGCAGTGACG ACACGGAGCT GCGCTACTCA 
ACCCAGTACG AGGAGCGCCT GGACCCCTTC TCCTCCTTCA GCAAGCGGGA GCGGCAGCGG 
AAATACCTGA GCCTGAGCCC GTGGGACAAG GCCACGCTCA GCATGGGGCG GCTGGTTCTC 
TCCAACAAGA TGGCCCGCAC CATCGGCTTC TTCTACACGC TGTTTCTGCA CCTCCTGGTC 
TTCCTGGTGC TCTACAAGCT GGCATGGAGC GAGAGCATGG AGCGGGACTG TGCCACCTTC 
TGCGCCAAGA AGTTCGCCGA CCACCTGCAC AAGTTCCACG AGAATGACAA GGGGGTGGCC 
GCCGGCGACC TGTGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131188.1
CDS	16..2316
Translation	MESAAGPLRNAYPDRVLESKAPGVPLDSSVPGRGPRTQPQAPRGDRAGAPHPSPEPHPCPGSAFLPATPHPGEQRALGVRLGPASSALAAPGQPRDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGRCAELQVHVTELTATAAEQRELIARLEQDLSTIQSIQRPDAEGAAELGLEKIPEPIKEATALFYGPSAPASSLPEGQVDSLLSIISSQRERFRARNQELEAENRLAQHTIQALQTELDSLRADNIKLFEKIKFLQSYPGRGGGSDDTELRYSTQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTIGFFYTLFLHLLVFLVLYKLAWSESMERDCATFCAKKFADHLHKFHENDKGVAAGDLWQ

Target ORF information:

RefSeq Version	XM_025275403.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275403.1

ORF Insert Sequence:

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGCGCTGC GCGGAGCTGC AGGTCCACGT CACCGAGCTC 
ACGGCCACGG CAGCTGAGCA GAGGGAGCTG ATCGCCCGCT TGGAGCAGGA CCTCAGCACC 
ATCCAGTCCA TCCAGCGGCC AGACGCCGAG GGCGCAGCCG AGCTCGGCCT GGAGAAGATT 
CCCGAGCCCA TCAAGGAGGC CACCGCCCTG TTCTATGGAC CCTCAGCGCC TGCCAGCTCC 
CTCCCCGAGG GCCAGGTGGA CTCGCTGCTC TCCATCATCT CCAGCCAGAG GGAGCGCTTC 
CGGGCCCGGA ACCAGGAGCT GGAGGCGGAG AACCGCCTGG CCCAGCATAC CATCCAAGCC 
CTGCAGACCG AGCTGGACAG CCTGCGCGCG GACAACATCA AGCTCTTTGA GAAGATCAAG 
TTCCTGCAGA GCTACCCTGG CCGGGGCGGC GGCAGTGACG ACACGGAGCT GCGCTACTCA 
ACCCAGTACG AGGAGCGCCT GGACCCCTTC TCCTCCTTCA GCAAGCGGGA GCGGCAGCGG 
AAATACCTGA GCCTGAGCCC GTGGGACAAG GCCACGCTCA GCATGGGGCG GCTGGTTCTC 
TCCAACAAGA TGGCCCGCAC CATCGGCTTC TTCTACACGC TGTTTCTGCA CCTCCTGGTC 
TTCCTGGTGC TCTACAAGCT GGCATGGAGC GAGAGCATGG AGCGGGACTG TGCCACCTTC 
TGCGCCAAGA AGTTCGCCGA CCACCTGCAC AAGTTCCACG AGAATGACAA GGGGGTGGCC 
GCCGGCGACC TGTGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275404.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131189.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu60249
Clone ID Related Accession (Same CDS sequence)	XM_025275404.1
Accession Version	XM_025275404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGGCGC 
TGCGCGGAGC TGCAGGTCCA CGTCACCGAG CTCACGGCCA CGGCAGCTGA GCAGAGGGAG 
CTGATCGCCC GCTTGGAGCA GGACCTCAGC ACCATCCAGT CCATCCAGCG GCCAGACGCC 
GAGGGCGCAG CCGAGCTCGG CCTGGAGAAG ATTCCCGAGC CCATCAAGGA GGCCACCGCC 
CTGTTCTATG GACCCTCAGC GCCTGCCAGC TCCCTCCCCG AGGGCCAGGT GGACTCGCTG 
CTCTCCATCA TCTCCAGCCA GAGGGAGCGC TTCCGGGCCC GGAACCAGGA GCTGGAGGCG 
GAGAACCGCC TGGCCCAGCA TACCATCCAA GCCCTGCAGA CCGAGCTGGA CAGCCTGCGC 
GCGGACAACA TCAAGCTCTT TGAGAAGATC AAGTTCCTGC AGAGCTACCC TGGCCGGGGC 
GGCGGCAGTG ACGACACGGA GCTGCGCTAC TCAACCCAGT ACGAGGAGCG CCTGGACCCC 
TTCTCCTCCT TCAGCAAGCG GGAGCGGCAG CGGAAATACC TGAGCCTGAG CCCGTGGGAC 
AAGGCCACGC TCAGCATGGG GCGGCTGGTT CTCTCCAACA AGATGGCCCG CACCATCGGC 
TTCTTCTACA CGCTGTTTCT GCACCTCCTG GTCTTCCTGG TGCTCTACAA GCTGGCATGG 
AGCGAGAGCA TGGAGCGGGA CTGTGCCACC TTCTGCGCCA AGAAGTTCGC CGACCACCTG 
CACAAGTTCC ACGAGAATGA CAAGGGGGTG GCCGCCGGCG ACCTGTGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131189.1
CDS	143..2176
Translation	MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGRCAELQVHVTELTATAAEQRELIARLEQDLSTIQSIQRPDAEGAAELGLEKIPEPIKEATALFYGPSAPASSLPEGQVDSLLSIISSQRERFRARNQELEAENRLAQHTIQALQTELDSLRADNIKLFEKIKFLQSYPGRGGGSDDTELRYSTQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTIGFFYTLFLHLLVFLVLYKLAWSESMERDCATFCAKKFADHLHKFHENDKGVAAGDLWQ

Target ORF information:

RefSeq Version	XM_025275404.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275404.1

ORF Insert Sequence:

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGGCGC 
TGCGCGGAGC TGCAGGTCCA CGTCACCGAG CTCACGGCCA CGGCAGCTGA GCAGAGGGAG 
CTGATCGCCC GCTTGGAGCA GGACCTCAGC ACCATCCAGT CCATCCAGCG GCCAGACGCC 
GAGGGCGCAG CCGAGCTCGG CCTGGAGAAG ATTCCCGAGC CCATCAAGGA GGCCACCGCC 
CTGTTCTATG GACCCTCAGC GCCTGCCAGC TCCCTCCCCG AGGGCCAGGT GGACTCGCTG 
CTCTCCATCA TCTCCAGCCA GAGGGAGCGC TTCCGGGCCC GGAACCAGGA GCTGGAGGCG 
GAGAACCGCC TGGCCCAGCA TACCATCCAA GCCCTGCAGA CCGAGCTGGA CAGCCTGCGC 
GCGGACAACA TCAAGCTCTT TGAGAAGATC AAGTTCCTGC AGAGCTACCC TGGCCGGGGC 
GGCGGCAGTG ACGACACGGA GCTGCGCTAC TCAACCCAGT ACGAGGAGCG CCTGGACCCC 
TTCTCCTCCT TCAGCAAGCG GGAGCGGCAG CGGAAATACC TGAGCCTGAG CCCGTGGGAC 
AAGGCCACGC TCAGCATGGG GCGGCTGGTT CTCTCCAACA AGATGGCCCG CACCATCGGC 
TTCTTCTACA CGCTGTTTCT GCACCTCCTG GTCTTCCTGG TGCTCTACAA GCTGGCATGG 
AGCGAGAGCA TGGAGCGGGA CTGTGCCACC TTCTGCGCCA AGAAGTTCGC CGACCACCTG 
CACAAGTTCC ACGAGAATGA CAAGGGGGTG GCCGCCGGCG ACCTGTGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275406.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131191.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu60250
Clone ID Related Accession (Same CDS sequence)	XM_025275406.1
Accession Version	XM_025275406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGCCATAGAG GTGCTGACCC GCTCCAGCCT GGAAGCTGAG 
TTGGCCGCCA AGGAGCGGGA GATCGCCCAG CTGGTGGAGG ACGTGCAGAG ACTCCAGGCC 
AACCTCAGCA AGCTGCGCGA GAACTCAGCC AGCCAGATCT CCCAGCTCGA GCAGCAGCTG 
AGCGCCAAGA ACAGCACCCT CAAGCAACTG GAAGAAAAAC TCAAGGGACA GGCTGACTAT 
GAAGAGGTGA AGAAAGAGCT AAACATCCTG AAGTCCATGG AGTTTGCGCC GGCCGAGGGA 
GCTGGGACGC AGGACGCATC CAGGCCCCTG GAGGTGCTGC TGCTGGAGAA GAACCGCTCG 
CTGCAGTCCG AGAACGCCGC GCTGCGCATC TCCAACAGCG ACCTGAGCGG GCGCTGCGCG 
GAGCTGCAGG TCCACGTCAC CGAGCTCACG GCCACGGCAG CTGAGCAGAG GGAGCTGATC 
GCCCGCTTGG AGCAGGACCT CAGCACCATC CAGTCCATCC AGCGGCCAGA CGCCGAGGGC 
GCAGCCGAGC TCGGCCTGGA GAAGATTCCC GAGCCCATCA AGGAGGCCAC CGCCCTGTTC 
TATGGACCCT CAGCGCCTGC CAGCTCCCTC CCCGAGGGCC AGGTGGACTC GCTGCTCTCC 
ATCATCTCCA GCCAGAGGGA GCGCTTCCGG GCCCGGAACC AGGAGCTGGA GGCGGAGAAC 
CGCCTGGCCC AGCATACCAT CCAAGCCCTG CAGACCGAGC TGGACAGCCT GCGCGCGGAC 
AACATCAAGC TCTTTGAGAA GATCAAGTTC CTGCAGAGCT ACCCTGGCCG GGGCGGCGGC 
AGTGACGACA CGGAGCTGCG CTACTCAACC CAGTACGAGG AGCGCCTGGA CCCCTTCTCC 
TCCTTCAGCA AGCGGGAGCG GCAGCGGAAA TACCTGAGCC TGAGCCCGTG GGACAAGGCC 
ACGCTCAGCA TGGGGCGGCT GGTTCTCTCC AACAAGATGG CCCGCACCAT CGGCTTCTTC 
TACACGCTGT TTCTGCACCT CCTGGTCTTC CTGGTGCTCT ACAAGCTGGC ATGGAGCGAG 
AGCATGGAGC GGGACTGTGC CACCTTCTGC GCCAAGAAGT TCGCCGACCA CCTGCACAAG 
TTCCACGAGA ATGACAAGGG GGTGGCCGCC GGCGACCTGT GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131191.1
CDS	145..2172
Translation	MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGRCAELQVHVTELTATAAEQRELIARLEQDLSTIQSIQRPDAEGAAELGLEKIPEPIKEATALFYGPSAPASSLPEGQVDSLLSIISSQRERFRARNQELEAENRLAQHTIQALQTELDSLRADNIKLFEKIKFLQSYPGRGGGSDDTELRYSTQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTIGFFYTLFLHLLVFLVLYKLAWSESMERDCATFCAKKFADHLHKFHENDKGVAAGDLWQ

Target ORF information:

RefSeq Version	XM_025275406.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275406.1

ORF Insert Sequence:

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGCCATAGAG GTGCTGACCC GCTCCAGCCT GGAAGCTGAG 
TTGGCCGCCA AGGAGCGGGA GATCGCCCAG CTGGTGGAGG ACGTGCAGAG ACTCCAGGCC 
AACCTCAGCA AGCTGCGCGA GAACTCAGCC AGCCAGATCT CCCAGCTCGA GCAGCAGCTG 
AGCGCCAAGA ACAGCACCCT CAAGCAACTG GAAGAAAAAC TCAAGGGACA GGCTGACTAT 
GAAGAGGTGA AGAAAGAGCT AAACATCCTG AAGTCCATGG AGTTTGCGCC GGCCGAGGGA 
GCTGGGACGC AGGACGCATC CAGGCCCCTG GAGGTGCTGC TGCTGGAGAA GAACCGCTCG 
CTGCAGTCCG AGAACGCCGC GCTGCGCATC TCCAACAGCG ACCTGAGCGG GCGCTGCGCG 
GAGCTGCAGG TCCACGTCAC CGAGCTCACG GCCACGGCAG CTGAGCAGAG GGAGCTGATC 
GCCCGCTTGG AGCAGGACCT CAGCACCATC CAGTCCATCC AGCGGCCAGA CGCCGAGGGC 
GCAGCCGAGC TCGGCCTGGA GAAGATTCCC GAGCCCATCA AGGAGGCCAC CGCCCTGTTC 
TATGGACCCT CAGCGCCTGC CAGCTCCCTC CCCGAGGGCC AGGTGGACTC GCTGCTCTCC 
ATCATCTCCA GCCAGAGGGA GCGCTTCCGG GCCCGGAACC AGGAGCTGGA GGCGGAGAAC 
CGCCTGGCCC AGCATACCAT CCAAGCCCTG CAGACCGAGC TGGACAGCCT GCGCGCGGAC 
AACATCAAGC TCTTTGAGAA GATCAAGTTC CTGCAGAGCT ACCCTGGCCG GGGCGGCGGC 
AGTGACGACA CGGAGCTGCG CTACTCAACC CAGTACGAGG AGCGCCTGGA CCCCTTCTCC 
TCCTTCAGCA AGCGGGAGCG GCAGCGGAAA TACCTGAGCC TGAGCCCGTG GGACAAGGCC 
ACGCTCAGCA TGGGGCGGCT GGTTCTCTCC AACAAGATGG CCCGCACCAT CGGCTTCTTC 
TACACGCTGT TTCTGCACCT CCTGGTCTTC CTGGTGCTCT ACAAGCTGGC ATGGAGCGAG 
AGCATGGAGC GGGACTGTGC CACCTTCTGC GCCAAGAAGT TCGCCGACCA CCTGCACAAG 
TTCCACGAGA ATGACAAGGG GGTGGCCGCC GGCGACCTGT GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275402.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131187.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X6, mRNA.

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CloneID	OBu60247
Clone ID Related Accession (Same CDS sequence)	XM_025275402.1
Accession Version	XM_025275402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGTCCA 
TACAGCACAA ACTCCATATC TTCCCAAAGT CCATTACAAC AAAGCCCAGA TGTAAATGGC 
ATGGCCCCAT CTCCCAGCCA GTCAGAAAGT GCTGGGAGCG TCTCCGAGGG CGAGGAGATA 
GACACTGCAG AAATCGCCCG GCAGGTGAAA GAGCAGCTGA TCAAGCACAA TATCGGACAG 
CGCATTTTCG GACACTACGT CTTAGGACTG TCTCAGGGGT CCGTGAGCGA GATCCTGGCC 
CGGCCCAAGC CGTGGAACAA ACTGACGGTG CGCGGCAAGG AGCCGTTTCA CAAGATGAAG 
CAGTTCCTCT CAGACGAGCA GAACATCCTG GCTCTTCGCA GCATCCAAGG CAGACAAAGA 
GAGAATCCAG GCCAGAGCCT GAACAGACTC TTTCAGGAAG TACCGAAACG AAGAAATGGG 
TCTGAAGGTA ACATCACCAC CCGGATCCGA GCCTCCGAGA CCGGGTCTGA CGAAGCCATC 
AAGTCCATCT TGGAACAAGC CAAGAGGGAG CTGCAAGTCC AGAAAACCGC AGAGCCGGCC 
CAGCCTTCCT CGGCATCCAG TGGCGGGAGC GCAGACGACG CCATCCGCTC CATCCTGCAG 
CAGGCCCGCC GGGAGATGGA GGCCCAGCAG GCCGCCCTCG AGCCTGCCTT AAAGCCCGCG 
CCGCCATCCC AGACAGACAT CGCCCTCCTG ACCCCCAAGC TGATACCCGC CTCGCCCATG 
TCGTCGGTGT CCAGTTACTC CCCTTTAGCC GTCTCCCTGA AGAAGCCCCC CTCGGCTCCC 
GACGCCAGCG CCCCTGCCCT GCCCAACCCG CCAGCCCTGA AGAAGGAGTC CCAGGATGCT 
CCCGGGCCGG ACCTCCCAGG GGCGGCCGAC CCCGCCGCAC AGGGGGTCTT GAGACACGTG 
AAGAGTGAGC TCGGCCGCGG CACCGTGTGG AAGGATCACT GGTGGAGCGC CGTCCAGCCC 
GAGAGGAAGA GCAGCGCTCC GCCCGAAGAC CCCAAGGCCG AAGACGCCAG CAGCAGCAAA 
GAAAAAGGCA GCGGCGGGCA GGCGCGGGCG GAGCGCAGCC AGCTCCAGGG CCCCTCCTCC 
TCCGAGTACT GGAAGGACTG GCCCAGCGCA GAGTCGCCCT ACTCCCAGAG CTCAGAGCTG 
AGCCTGACCG GAGCCAGCCG CAGCGAGACG CCCCAGAACA GCCCACTGCC CTCCTCGCCC 
ATTGTGCCCC TGTCGAAGCC TACCAAGCCC TCAGTCCCGC CACTGACCCC CGAGCAGTAC 
GAGATCTACA TGTACCAGGA GGTGGACACC ATCGAGCTCA CCCGGCAGGT GAAGGAGAAG 
CTGGCCAAGA ACGGCATCTG TCAGAGGATC TTCGGGGAGA AGGTGCTAGG CCTGTCACAG 
GGCAGTGTCA GTGACATGCT GTCCAGGCCG AAGCCATGGA GCAAGCTGAC CCAGAAAGGA 
CGAGAACCCT TCATACGGAT GCAGCTCTGG CTGAACGGCG AGCTGGGCCA GGGCGTCCTG 
CCCGTCCAAG GGCAGCAGCA AGGGCCAGTC TTGCACTCCG TGACATCACT ACAAGACCCC 
CTGCAGCAGG GCTGCGTGAG CTCAGAAAGC ACTCCAAAGA CCTCCGCCAG CTGCAGCCCT 
GCACCCGAGT CACCCATGAG CTCCAGTGAG TCAGTGAAGA GTCTGACTGA ACTGGTCCAG 
CAGCCCTGCC CCCCCATCGA AACCAGTAAG GACAGCAAAC CTCCAGAACC CAGCGACCCA 
CCCGCCTCAG ACTCCCAGCC CGCCACCCCA CTGCCTCTCT CTGGACACTC AGCACTCAGC 
ATTCAAGAGC TGGTGGCCAT GTCTCCAGAG CTGGACACCT ACGGCATCAC CAAGAGGGTC 
AAGGAGGTGT TGACCGACAA CAACCTCGGT CAGCGCTTAT TTGGGGAGAC CATCCTGGGG 
CTCACCCAAG GCTCTGTCTC TGACCTCCTT GCTCGCCCCA AGCCCTGGCA CAAGCTCAGT 
CTGAAGGGAC GGGAGCCCTT TGTCCGGATG CAGCTGTGGC TGAATGACCC CAACAACGTG 
GAGAAGCTGA TGGACATGAA ACGGATGGAA AAGAAAGCCT ACATGAAGCG GCGGCACAGC 
TCGGTCAGCG ACAGCCAGCC CTGTGAGCCC CCGTCCGTTG GCATCGACTA CAGCCAGGGC 
GCCAGCCCCC AGCCCCAGCA CCAGCTGAAG AAACCCCGAG TAGTGCTGGC CCCCGAAGAG 
AAAGAAGCTC TGAAACGAGC ATATCAGCAA AAGCCATACC CGTCACCAAA AACCATCGAA 
GAACTCGCCA CCCAGCTCAA CTTGAAAACC AGCACCGTCA TCAACTGGTT CCACAATTAC 
AGGTCTCGGA TCCGCAGAGA ACTGTTCATT GAGGAGATTC AGGCCGGGAG CCAGGGCCAG 
GCCGGGGCCA GCGATTCGCC GTCCGCCCGG AGCGGCCGGG CCGCACCCAG CTCAGAAGGC 
GACAGCTGCG ACGGCGTGGA GGCCGCCGAG GGCCCAGGCG CCGCGGACGC CGAAGAGTCG 
GGCGGCCCCG CGGCCGCCGC CAAGTCTCAG GGAGGGCCGG CCGAGGCGGC CGCGGCCCCA 
GAGGAGCGGG AGGAGGCGCC GCGGCCGGCG GAGAAGGCAG AGCCGCCGCC CTCGGGGACC 
CCGGTCCCGG ACGCCGCCGC CGACGAAGGC CGGCCCCGGG CGCCGCCGCC GCCGCCACCC 
GAGGGTCCCG CGGACGGCCC GGGGCCCGTG CCAAACCCCG CCGCCGCCGC ACCCGCGGCC 
GGGGAGGACG CCGCTACCTC AGCCGCCCCG CCCGGCGAGG GCCCCTGCCG GGCCCGGGAC 
GGCGCCGACA GGAGTTCCGC TTTGCCAAGC ACCAGCGCGC CCGCGGCTGC CCGCAGACCC 
AGCTCGCTGC AGAGCCTCTT CGGCCTCCCA GAGGCGGCGG GCGCCAGGGA CTCGCGAGAC 
AACCCCTTGA GGAAGAAGAA GGCCGCGAAC TTGAACAGCA TAATCCACCG CCTGGAGAAG 
GCTGCCAGCC GCGAGGAACC CATCGAATGG GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131187.1
CDS	166..4464
Translation	MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275402.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275402.1

ORF Insert Sequence:

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGTCCA 
TACAGCACAA ACTCCATATC TTCCCAAAGT CCATTACAAC AAAGCCCAGA TGTAAATGGC 
ATGGCCCCAT CTCCCAGCCA GTCAGAAAGT GCTGGGAGCG TCTCCGAGGG CGAGGAGATA 
GACACTGCAG AAATCGCCCG GCAGGTGAAA GAGCAGCTGA TCAAGCACAA TATCGGACAG 
CGCATTTTCG GACACTACGT CTTAGGACTG TCTCAGGGGT CCGTGAGCGA GATCCTGGCC 
CGGCCCAAGC CGTGGAACAA ACTGACGGTG CGCGGCAAGG AGCCGTTTCA CAAGATGAAG 
CAGTTCCTCT CAGACGAGCA GAACATCCTG GCTCTTCGCA GCATCCAAGG CAGACAAAGA 
GAGAATCCAG GCCAGAGCCT GAACAGACTC TTTCAGGAAG TACCGAAACG AAGAAATGGG 
TCTGAAGGTA ACATCACCAC CCGGATCCGA GCCTCCGAGA CCGGGTCTGA CGAAGCCATC 
AAGTCCATCT TGGAACAAGC CAAGAGGGAG CTGCAAGTCC AGAAAACCGC AGAGCCGGCC 
CAGCCTTCCT CGGCATCCAG TGGCGGGAGC GCAGACGACG CCATCCGCTC CATCCTGCAG 
CAGGCCCGCC GGGAGATGGA GGCCCAGCAG GCCGCCCTCG AGCCTGCCTT AAAGCCCGCG 
CCGCCATCCC AGACAGACAT CGCCCTCCTG ACCCCCAAGC TGATACCCGC CTCGCCCATG 
TCGTCGGTGT CCAGTTACTC CCCTTTAGCC GTCTCCCTGA AGAAGCCCCC CTCGGCTCCC 
GACGCCAGCG CCCCTGCCCT GCCCAACCCG CCAGCCCTGA AGAAGGAGTC CCAGGATGCT 
CCCGGGCCGG ACCTCCCAGG GGCGGCCGAC CCCGCCGCAC AGGGGGTCTT GAGACACGTG 
AAGAGTGAGC TCGGCCGCGG CACCGTGTGG AAGGATCACT GGTGGAGCGC CGTCCAGCCC 
GAGAGGAAGA GCAGCGCTCC GCCCGAAGAC CCCAAGGCCG AAGACGCCAG CAGCAGCAAA 
GAAAAAGGCA GCGGCGGGCA GGCGCGGGCG GAGCGCAGCC AGCTCCAGGG CCCCTCCTCC 
TCCGAGTACT GGAAGGACTG GCCCAGCGCA GAGTCGCCCT ACTCCCAGAG CTCAGAGCTG 
AGCCTGACCG GAGCCAGCCG CAGCGAGACG CCCCAGAACA GCCCACTGCC CTCCTCGCCC 
ATTGTGCCCC TGTCGAAGCC TACCAAGCCC TCAGTCCCGC CACTGACCCC CGAGCAGTAC 
GAGATCTACA TGTACCAGGA GGTGGACACC ATCGAGCTCA CCCGGCAGGT GAAGGAGAAG 
CTGGCCAAGA ACGGCATCTG TCAGAGGATC TTCGGGGAGA AGGTGCTAGG CCTGTCACAG 
GGCAGTGTCA GTGACATGCT GTCCAGGCCG AAGCCATGGA GCAAGCTGAC CCAGAAAGGA 
CGAGAACCCT TCATACGGAT GCAGCTCTGG CTGAACGGCG AGCTGGGCCA GGGCGTCCTG 
CCCGTCCAAG GGCAGCAGCA AGGGCCAGTC TTGCACTCCG TGACATCACT ACAAGACCCC 
CTGCAGCAGG GCTGCGTGAG CTCAGAAAGC ACTCCAAAGA CCTCCGCCAG CTGCAGCCCT 
GCACCCGAGT CACCCATGAG CTCCAGTGAG TCAGTGAAGA GTCTGACTGA ACTGGTCCAG 
CAGCCCTGCC CCCCCATCGA AACCAGTAAG GACAGCAAAC CTCCAGAACC CAGCGACCCA 
CCCGCCTCAG ACTCCCAGCC CGCCACCCCA CTGCCTCTCT CTGGACACTC AGCACTCAGC 
ATTCAAGAGC TGGTGGCCAT GTCTCCAGAG CTGGACACCT ACGGCATCAC CAAGAGGGTC 
AAGGAGGTGT TGACCGACAA CAACCTCGGT CAGCGCTTAT TTGGGGAGAC CATCCTGGGG 
CTCACCCAAG GCTCTGTCTC TGACCTCCTT GCTCGCCCCA AGCCCTGGCA CAAGCTCAGT 
CTGAAGGGAC GGGAGCCCTT TGTCCGGATG CAGCTGTGGC TGAATGACCC CAACAACGTG 
GAGAAGCTGA TGGACATGAA ACGGATGGAA AAGAAAGCCT ACATGAAGCG GCGGCACAGC 
TCGGTCAGCG ACAGCCAGCC CTGTGAGCCC CCGTCCGTTG GCATCGACTA CAGCCAGGGC 
GCCAGCCCCC AGCCCCAGCA CCAGCTGAAG AAACCCCGAG TAGTGCTGGC CCCCGAAGAG 
AAAGAAGCTC TGAAACGAGC ATATCAGCAA AAGCCATACC CGTCACCAAA AACCATCGAA 
GAACTCGCCA CCCAGCTCAA CTTGAAAACC AGCACCGTCA TCAACTGGTT CCACAATTAC 
AGGTCTCGGA TCCGCAGAGA ACTGTTCATT GAGGAGATTC AGGCCGGGAG CCAGGGCCAG 
GCCGGGGCCA GCGATTCGCC GTCCGCCCGG AGCGGCCGGG CCGCACCCAG CTCAGAAGGC 
GACAGCTGCG ACGGCGTGGA GGCCGCCGAG GGCCCAGGCG CCGCGGACGC CGAAGAGTCG 
GGCGGCCCCG CGGCCGCCGC CAAGTCTCAG GGAGGGCCGG CCGAGGCGGC CGCGGCCCCA 
GAGGAGCGGG AGGAGGCGCC GCGGCCGGCG GAGAAGGCAG AGCCGCCGCC CTCGGGGACC 
CCGGTCCCGG ACGCCGCCGC CGACGAAGGC CGGCCCCGGG CGCCGCCGCC GCCGCCACCC 
GAGGGTCCCG CGGACGGCCC GGGGCCCGTG CCAAACCCCG CCGCCGCCGC ACCCGCGGCC 
GGGGAGGACG CCGCTACCTC AGCCGCCCCG CCCGGCGAGG GCCCCTGCCG GGCCCGGGAC 
GGCGCCGACA GGAGTTCCGC TTTGCCAAGC ACCAGCGCGC CCGCGGCTGC CCGCAGACCC 
AGCTCGCTGC AGAGCCTCTT CGGCCTCCCA GAGGCGGCGG GCGCCAGGGA CTCGCGAGAC 
AACCCCTTGA GGAAGAAGAA GGCCGCGAAC TTGAACAGCA TAATCCACCG CCTGGAGAAG 
GCTGCCAGCC GCGAGGAACC CATCGAATGG GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275397.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131182.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu60242
Clone ID Related Accession (Same CDS sequence)	XM_025275397.1
Accession Version	XM_025275397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGAGAATC CAGGCCAGAG CCTGAACAGA 
CTCTTTCAGG AAGTACCGAA ACGAAGAAAT GGGTCTGAAG GTAACATCAC CACCCGGATC 
CGAGCCTCCG AGACCGGGTC TGACGAAGCC ATCAAGTCCA TCTTGGAACA AGCCAAGAGG 
GAGCTGCAAG TCCAGAAAAC CGCAGAGCCG GCCCAGCCTT CCTCGGCATC CAGTGGCGGG 
AGCGCAGACG ACGCCATCCG CTCCATCCTG CAGCAGGCCC GCCGGGAGAT GGAGGCCCAG 
CAGGCCGCCC TCGAGCCTGC CTTAAAGCCC GCGCCGCCAT CCCAGACAGA CATCGCCCTC 
CTGACCCCCA AGCTGATACC CGCCTCGCCC ATGTCGTCGG TGTCCAGTTA CTCCCCTTTA 
GCCGTCTCCC TGAAGAAGCC CCCCTCGGCT CCCGACGCCA GCGCCCCTGC CCTGCCCAAC 
CCGCCAGCCC TGAAGAAGGA GTCCCAGGAT GCTCCCGGGC CGGACCTCCC AGGGGCGGCC 
GACCCCGCCG CACAGGGGGT CTTGAGACAC GTGAAGAGTG AGCTCGGCCG CGGCACCGTG 
TGGAAGGATC ACTGGTGGAG CGCCGTCCAG CCCGAGAGGA AGAGCAGCGC TCCGCCCGAA 
GACCCCAAGG CCGAAGACGC CAGCAGCAGC AAAGAAAAAG GCAGCGGCGG GCAGGCGCGG 
GCGGAGCGCA GCCAGCTCCA GGGCCCCTCC TCCTCCGAGT ACTGGAAGGA CTGGCCCAGC 
GCAGAGTCGC CCTACTCCCA GAGCTCAGAG CTGAGCCTGA CCGGAGCCAG CCGCAGCGAG 
ACGCCCCAGA ACAGCCCACT GCCCTCCTCG CCCATTGTGC CCCTGTCGAA GCCTACCAAG 
CCCTCAGTCC CGCCACTGAC CCCCGAGCAG TACGAGATCT ACATGTACCA GGAGGTGGAC 
ACCATCGAGC TCACCCGGCA GGTGAAGGAG AAGCTGGCCA AGAACGGCAT CTGTCAGAGG 
ATCTTCGGGG AGAAGGTGCT AGGCCTGTCA CAGGGCAGTG TCAGTGACAT GCTGTCCAGG 
CCGAAGCCAT GGAGCAAGCT GACCCAGAAA GGACGAGAAC CCTTCATACG GATGCAGCTC 
TGGCTGAACG GCGAGCTGGG CCAGGGCGTC CTGCCCGTCC AAGGGCAGCA GCAAGGGCCA 
GTCTTGCACT CCGTGACATC ACTACAAGAC CCCCTGCAGC AGGGCTGCGT GAGCTCAGAA 
AGCACTCCAA AGACCTCCGC CAGCTGCAGC CCTGCACCCG AGTCACCCAT GAGCTCCAGT 
GAGTCAGTGA AGAGTCTGAC TGAACTGGTC CAGCAGCCCT GCCCCCCCAT CGAAACCAGT 
AAGGACAGCA AACCTCCAGA ACCCAGCGAC CCACCCGCCT CAGACTCCCA GCCCGCCACC 
CCACTGCCTC TCTCTGGACA CTCAGCACTC AGCATTCAAG AGCTGGTGGC CATGTCTCCA 
GAGCTGGACA CCTACGGCAT CACCAAGAGG GTCAAGGAGG TGTTGACCGA CAACAACCTC 
GGTCAGCGCT TATTTGGGGA GACCATCCTG GGGCTCACCC AAGGCTCTGT CTCTGACCTC 
CTTGCTCGCC CCAAGCCCTG GCACAAGCTC AGTCTGAAGG GACGGGAGCC CTTTGTCCGG 
ATGCAGCTGT GGCTGAATGA CCCCAACAAC GTGGAGAAGC TGATGGACAT GAAACGGATG 
GAAAAGAAAG CCTACATGAA GCGGCGGCAC AGCTCGGTCA GCGACAGCCA GCCCTGTGAG 
CCCCCGTCCG TTGGCATCGA CTACAGCCAG GGCGCCAGCC CCCAGCCCCA GCACCAGCTG 
AAGAAACCCC GAGTAGTGCT GGCCCCCGAA GAGAAAGAAG CTCTGAAACG AGCATATCAG 
CAAAAGCCAT ACCCGTCACC AAAAACCATC GAAGAACTCG CCACCCAGCT CAACTTGAAA 
ACCAGCACCG TCATCAACTG GTTCCACAAT TACAGGTCTC GGATCCGCAG AGAACTGTTC 
ATTGAGGAGA TTCAGGCCGG GAGCCAGGGC CAGGCCGGGG CCAGCGATTC GCCGTCCGCC 
CGGAGCGGCC GGGCCGCACC CAGCTCAGAA GGCGACAGCT GCGACGGCGT GGAGGCCGCC 
GAGGGCCCAG GCGCCGCGGA CGCCGAAGAG TCGGGCGGCC CCGCGGCCGC CGCCAAGTCT 
CAGGGAGGGC CGGCCGAGGC GGCCGCGGCC CCAGAGGAGC GGGAGGAGGC GCCGCGGCCG 
GCGGAGAAGG CAGAGCCGCC GCCCTCGGGG ACCCCGGTCC CGGACGCCGC CGCCGACGAA 
GGCCGGCCCC GGGCGCCGCC GCCGCCGCCA CCCGAGGGTC CCGCGGACGG CCCGGGGCCC 
GTGCCAAACC CCGCCGCCGC CGCACCCGCG GCCGGGGAGG ACGCCGCTAC CTCAGCCGCC 
CCGCCCGGCG AGGGCCCCTG CCGGGCCCGG GACGGCGCCG ACAGGAGTTC CGCTTTGCCA 
AGCACCAGCG CGCCCGCGGC TGCCCGCAGA CCCAGCTCGC TGCAGAGCCT CTTCGGCCTC 
CCAGAGGCGG CGGGCGCCAG GGACTCGCGA GACAACCCCT TGAGGAAGAA GAAGGCCGCG 
AACTTGAACA GCATAATCCA CCGCCTGGAG AAGGCTGCCA GCCGCGAGGA ACCCATCGAA 
TGGGAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131182.1
CDS	16..4887
Translation	MESAAGPLRNAYPDRVLESKAPGVPLDSSVPGRGPRTQPQAPRGDRAGAPHPSPEPHPCPGSAFLPATPHPGEQRALGVRLGPASSALAAPGQPRDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275397.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275397.1

ORF Insert Sequence:

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGAGAATC CAGGCCAGAG CCTGAACAGA 
CTCTTTCAGG AAGTACCGAA ACGAAGAAAT GGGTCTGAAG GTAACATCAC CACCCGGATC 
CGAGCCTCCG AGACCGGGTC TGACGAAGCC ATCAAGTCCA TCTTGGAACA AGCCAAGAGG 
GAGCTGCAAG TCCAGAAAAC CGCAGAGCCG GCCCAGCCTT CCTCGGCATC CAGTGGCGGG 
AGCGCAGACG ACGCCATCCG CTCCATCCTG CAGCAGGCCC GCCGGGAGAT GGAGGCCCAG 
CAGGCCGCCC TCGAGCCTGC CTTAAAGCCC GCGCCGCCAT CCCAGACAGA CATCGCCCTC 
CTGACCCCCA AGCTGATACC CGCCTCGCCC ATGTCGTCGG TGTCCAGTTA CTCCCCTTTA 
GCCGTCTCCC TGAAGAAGCC CCCCTCGGCT CCCGACGCCA GCGCCCCTGC CCTGCCCAAC 
CCGCCAGCCC TGAAGAAGGA GTCCCAGGAT GCTCCCGGGC CGGACCTCCC AGGGGCGGCC 
GACCCCGCCG CACAGGGGGT CTTGAGACAC GTGAAGAGTG AGCTCGGCCG CGGCACCGTG 
TGGAAGGATC ACTGGTGGAG CGCCGTCCAG CCCGAGAGGA AGAGCAGCGC TCCGCCCGAA 
GACCCCAAGG CCGAAGACGC CAGCAGCAGC AAAGAAAAAG GCAGCGGCGG GCAGGCGCGG 
GCGGAGCGCA GCCAGCTCCA GGGCCCCTCC TCCTCCGAGT ACTGGAAGGA CTGGCCCAGC 
GCAGAGTCGC CCTACTCCCA GAGCTCAGAG CTGAGCCTGA CCGGAGCCAG CCGCAGCGAG 
ACGCCCCAGA ACAGCCCACT GCCCTCCTCG CCCATTGTGC CCCTGTCGAA GCCTACCAAG 
CCCTCAGTCC CGCCACTGAC CCCCGAGCAG TACGAGATCT ACATGTACCA GGAGGTGGAC 
ACCATCGAGC TCACCCGGCA GGTGAAGGAG AAGCTGGCCA AGAACGGCAT CTGTCAGAGG 
ATCTTCGGGG AGAAGGTGCT AGGCCTGTCA CAGGGCAGTG TCAGTGACAT GCTGTCCAGG 
CCGAAGCCAT GGAGCAAGCT GACCCAGAAA GGACGAGAAC CCTTCATACG GATGCAGCTC 
TGGCTGAACG GCGAGCTGGG CCAGGGCGTC CTGCCCGTCC AAGGGCAGCA GCAAGGGCCA 
GTCTTGCACT CCGTGACATC ACTACAAGAC CCCCTGCAGC AGGGCTGCGT GAGCTCAGAA 
AGCACTCCAA AGACCTCCGC CAGCTGCAGC CCTGCACCCG AGTCACCCAT GAGCTCCAGT 
GAGTCAGTGA AGAGTCTGAC TGAACTGGTC CAGCAGCCCT GCCCCCCCAT CGAAACCAGT 
AAGGACAGCA AACCTCCAGA ACCCAGCGAC CCACCCGCCT CAGACTCCCA GCCCGCCACC 
CCACTGCCTC TCTCTGGACA CTCAGCACTC AGCATTCAAG AGCTGGTGGC CATGTCTCCA 
GAGCTGGACA CCTACGGCAT CACCAAGAGG GTCAAGGAGG TGTTGACCGA CAACAACCTC 
GGTCAGCGCT TATTTGGGGA GACCATCCTG GGGCTCACCC AAGGCTCTGT CTCTGACCTC 
CTTGCTCGCC CCAAGCCCTG GCACAAGCTC AGTCTGAAGG GACGGGAGCC CTTTGTCCGG 
ATGCAGCTGT GGCTGAATGA CCCCAACAAC GTGGAGAAGC TGATGGACAT GAAACGGATG 
GAAAAGAAAG CCTACATGAA GCGGCGGCAC AGCTCGGTCA GCGACAGCCA GCCCTGTGAG 
CCCCCGTCCG TTGGCATCGA CTACAGCCAG GGCGCCAGCC CCCAGCCCCA GCACCAGCTG 
AAGAAACCCC GAGTAGTGCT GGCCCCCGAA GAGAAAGAAG CTCTGAAACG AGCATATCAG 
CAAAAGCCAT ACCCGTCACC AAAAACCATC GAAGAACTCG CCACCCAGCT CAACTTGAAA 
ACCAGCACCG TCATCAACTG GTTCCACAAT TACAGGTCTC GGATCCGCAG AGAACTGTTC 
ATTGAGGAGA TTCAGGCCGG GAGCCAGGGC CAGGCCGGGG CCAGCGATTC GCCGTCCGCC 
CGGAGCGGCC GGGCCGCACC CAGCTCAGAA GGCGACAGCT GCGACGGCGT GGAGGCCGCC 
GAGGGCCCAG GCGCCGCGGA CGCCGAAGAG TCGGGCGGCC CCGCGGCCGC CGCCAAGTCT 
CAGGGAGGGC CGGCCGAGGC GGCCGCGGCC CCAGAGGAGC GGGAGGAGGC GCCGCGGCCG 
GCGGAGAAGG CAGAGCCGCC GCCCTCGGGG ACCCCGGTCC CGGACGCCGC CGCCGACGAA 
GGCCGGCCCC GGGCGCCGCC GCCGCCGCCA CCCGAGGGTC CCGCGGACGG CCCGGGGCCC 
GTGCCAAACC CCGCCGCCGC CGCACCCGCG GCCGGGGAGG ACGCCGCTAC CTCAGCCGCC 
CCGCCCGGCG AGGGCCCCTG CCGGGCCCGG GACGGCGCCG ACAGGAGTTC CGCTTTGCCA 
AGCACCAGCG CGCCCGCGGC TGCCCGCAGA CCCAGCTCGC TGCAGAGCCT CTTCGGCCTC 
CCAGAGGCGG CGGGCGCCAG GGACTCGCGA GACAACCCCT TGAGGAAGAA GAAGGCCGCG 
AACTTGAACA GCATAATCCA CCGCCTGGAG AAGGCTGCCA GCCGCGAGGA ACCCATCGAA 
TGGGAGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275398.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131183.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu60243
Clone ID Related Accession (Same CDS sequence)	XM_025275398.1
Accession Version	XM_025275398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGAGAATC CAGGCCAGAG CCTGAACAGA 
CTCTTTCAGG AAGTACCGAA ACGAAGAAAT GGGTCTGAAG GTAACATCAC CACCCGGATC 
CGAGCCTCCG AGACCGGGTC TGACGAAGCC ATCAAGTCCA TCTTGGAACA AGCCAAGAGG 
GAGCTGCAAG TCCAGAAAAC CGAGCCGGCC CAGCCTTCCT CGGCATCCAG TGGCGGGAGC 
GCAGACGACG CCATCCGCTC CATCCTGCAG CAGGCCCGCC GGGAGATGGA GGCCCAGCAG 
GCCGCCCTCG AGCCTGCCTT AAAGCCCGCG CCGCCATCCC AGACAGACAT CGCCCTCCTG 
ACCCCCAAGC TGATACCCGC CTCGCCCATG TCGTCGGTGT CCAGTTACTC CCCTTTAGCC 
GTCTCCCTGA AGAAGCCCCC CTCGGCTCCC GACGCCAGCG CCCCTGCCCT GCCCAACCCG 
CCAGCCCTGA AGAAGGAGTC CCAGGATGCT CCCGGGCCGG ACCTCCCAGG GGCGGCCGAC 
CCCGCCGCAC AGGGGGTCTT GAGACACGTG AAGAGTGAGC TCGGCCGCGG CACCGTGTGG 
AAGGATCACT GGTGGAGCGC CGTCCAGCCC GAGAGGAAGA GCAGCGCTCC GCCCGAAGAC 
CCCAAGGCCG AAGACGCCAG CAGCAGCAAA GAAAAAGGCA GCGGCGGGCA GGCGCGGGCG 
GAGCGCAGCC AGCTCCAGGG CCCCTCCTCC TCCGAGTACT GGAAGGACTG GCCCAGCGCA 
GAGTCGCCCT ACTCCCAGAG CTCAGAGCTG AGCCTGACCG GAGCCAGCCG CAGCGAGACG 
CCCCAGAACA GCCCACTGCC CTCCTCGCCC ATTGTGCCCC TGTCGAAGCC TACCAAGCCC 
TCAGTCCCGC CACTGACCCC CGAGCAGTAC GAGATCTACA TGTACCAGGA GGTGGACACC 
ATCGAGCTCA CCCGGCAGGT GAAGGAGAAG CTGGCCAAGA ACGGCATCTG TCAGAGGATC 
TTCGGGGAGA AGGTGCTAGG CCTGTCACAG GGCAGTGTCA GTGACATGCT GTCCAGGCCG 
AAGCCATGGA GCAAGCTGAC CCAGAAAGGA CGAGAACCCT TCATACGGAT GCAGCTCTGG 
CTGAACGGCG AGCTGGGCCA GGGCGTCCTG CCCGTCCAAG GGCAGCAGCA AGGGCCAGTC 
TTGCACTCCG TGACATCACT ACAAGACCCC CTGCAGCAGG GCTGCGTGAG CTCAGAAAGC 
ACTCCAAAGA CCTCCGCCAG CTGCAGCCCT GCACCCGAGT CACCCATGAG CTCCAGTGAG 
TCAGTGAAGA GTCTGACTGA ACTGGTCCAG CAGCCCTGCC CCCCCATCGA AACCAGTAAG 
GACAGCAAAC CTCCAGAACC CAGCGACCCA CCCGCCTCAG ACTCCCAGCC CGCCACCCCA 
CTGCCTCTCT CTGGACACTC AGCACTCAGC ATTCAAGAGC TGGTGGCCAT GTCTCCAGAG 
CTGGACACCT ACGGCATCAC CAAGAGGGTC AAGGAGGTGT TGACCGACAA CAACCTCGGT 
CAGCGCTTAT TTGGGGAGAC CATCCTGGGG CTCACCCAAG GCTCTGTCTC TGACCTCCTT 
GCTCGCCCCA AGCCCTGGCA CAAGCTCAGT CTGAAGGGAC GGGAGCCCTT TGTCCGGATG 
CAGCTGTGGC TGAATGACCC CAACAACGTG GAGAAGCTGA TGGACATGAA ACGGATGGAA 
AAGAAAGCCT ACATGAAGCG GCGGCACAGC TCGGTCAGCG ACAGCCAGCC CTGTGAGCCC 
CCGTCCGTTG GCATCGACTA CAGCCAGGGC GCCAGCCCCC AGCCCCAGCA CCAGCTGAAG 
AAACCCCGAG TAGTGCTGGC CCCCGAAGAG AAAGAAGCTC TGAAACGAGC ATATCAGCAA 
AAGCCATACC CGTCACCAAA AACCATCGAA GAACTCGCCA CCCAGCTCAA CTTGAAAACC 
AGCACCGTCA TCAACTGGTT CCACAATTAC AGGTCTCGGA TCCGCAGAGA ACTGTTCATT 
GAGGAGATTC AGGCCGGGAG CCAGGGCCAG GCCGGGGCCA GCGATTCGCC GTCCGCCCGG 
AGCGGCCGGG CCGCACCCAG CTCAGAAGGC GACAGCTGCG ACGGCGTGGA GGCCGCCGAG 
GGCCCAGGCG CCGCGGACGC CGAAGAGTCG GGCGGCCCCG CGGCCGCCGC CAAGTCTCAG 
GGAGGGCCGG CCGAGGCGGC CGCGGCCCCA GAGGAGCGGG AGGAGGCGCC GCGGCCGGCG 
GAGAAGGCAG AGCCGCCGCC CTCGGGGACC CCGGTCCCGG ACGCCGCCGC CGACGAAGGC 
CGGCCCCGGG CGCCGCCGCC GCCGCCACCC GAGGGTCCCG CGGACGGCCC GGGGCCCGTG 
CCAAACCCCG CCGCCGCCGC ACCCGCGGCC GGGGAGGACG CCGCTACCTC AGCCGCCCCG 
CCCGGCGAGG GCCCCTGCCG GGCCCGGGAC GGCGCCGACA GGAGTTCCGC TTTGCCAAGC 
ACCAGCGCGC CCGCGGCTGC CCGCAGACCC AGCTCGCTGC AGAGCCTCTT CGGCCTCCCA 
GAGGCGGCGG GCGCCAGGGA CTCGCGAGAC AACCCCTTGA GGAAGAAGAA GGCCGCGAAC 
TTGAACAGCA TAATCCACCG CCTGGAGAAG GCTGCCAGCC GCGAGGAACC CATCGAATGG 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131183.1
CDS	16..4884
Translation	MESAAGPLRNAYPDRVLESKAPGVPLDSSVPGRGPRTQPQAPRGDRAGAPHPSPEPHPCPGSAFLPATPHPGEQRALGVRLGPASSALAAPGQPRDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275398.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275398.1

ORF Insert Sequence:

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGAGAATC CAGGCCAGAG CCTGAACAGA 
CTCTTTCAGG AAGTACCGAA ACGAAGAAAT GGGTCTGAAG GTAACATCAC CACCCGGATC 
CGAGCCTCCG AGACCGGGTC TGACGAAGCC ATCAAGTCCA TCTTGGAACA AGCCAAGAGG 
GAGCTGCAAG TCCAGAAAAC CGAGCCGGCC CAGCCTTCCT CGGCATCCAG TGGCGGGAGC 
GCAGACGACG CCATCCGCTC CATCCTGCAG CAGGCCCGCC GGGAGATGGA GGCCCAGCAG 
GCCGCCCTCG AGCCTGCCTT AAAGCCCGCG CCGCCATCCC AGACAGACAT CGCCCTCCTG 
ACCCCCAAGC TGATACCCGC CTCGCCCATG TCGTCGGTGT CCAGTTACTC CCCTTTAGCC 
GTCTCCCTGA AGAAGCCCCC CTCGGCTCCC GACGCCAGCG CCCCTGCCCT GCCCAACCCG 
CCAGCCCTGA AGAAGGAGTC CCAGGATGCT CCCGGGCCGG ACCTCCCAGG GGCGGCCGAC 
CCCGCCGCAC AGGGGGTCTT GAGACACGTG AAGAGTGAGC TCGGCCGCGG CACCGTGTGG 
AAGGATCACT GGTGGAGCGC CGTCCAGCCC GAGAGGAAGA GCAGCGCTCC GCCCGAAGAC 
CCCAAGGCCG AAGACGCCAG CAGCAGCAAA GAAAAAGGCA GCGGCGGGCA GGCGCGGGCG 
GAGCGCAGCC AGCTCCAGGG CCCCTCCTCC TCCGAGTACT GGAAGGACTG GCCCAGCGCA 
GAGTCGCCCT ACTCCCAGAG CTCAGAGCTG AGCCTGACCG GAGCCAGCCG CAGCGAGACG 
CCCCAGAACA GCCCACTGCC CTCCTCGCCC ATTGTGCCCC TGTCGAAGCC TACCAAGCCC 
TCAGTCCCGC CACTGACCCC CGAGCAGTAC GAGATCTACA TGTACCAGGA GGTGGACACC 
ATCGAGCTCA CCCGGCAGGT GAAGGAGAAG CTGGCCAAGA ACGGCATCTG TCAGAGGATC 
TTCGGGGAGA AGGTGCTAGG CCTGTCACAG GGCAGTGTCA GTGACATGCT GTCCAGGCCG 
AAGCCATGGA GCAAGCTGAC CCAGAAAGGA CGAGAACCCT TCATACGGAT GCAGCTCTGG 
CTGAACGGCG AGCTGGGCCA GGGCGTCCTG CCCGTCCAAG GGCAGCAGCA AGGGCCAGTC 
TTGCACTCCG TGACATCACT ACAAGACCCC CTGCAGCAGG GCTGCGTGAG CTCAGAAAGC 
ACTCCAAAGA CCTCCGCCAG CTGCAGCCCT GCACCCGAGT CACCCATGAG CTCCAGTGAG 
TCAGTGAAGA GTCTGACTGA ACTGGTCCAG CAGCCCTGCC CCCCCATCGA AACCAGTAAG 
GACAGCAAAC CTCCAGAACC CAGCGACCCA CCCGCCTCAG ACTCCCAGCC CGCCACCCCA 
CTGCCTCTCT CTGGACACTC AGCACTCAGC ATTCAAGAGC TGGTGGCCAT GTCTCCAGAG 
CTGGACACCT ACGGCATCAC CAAGAGGGTC AAGGAGGTGT TGACCGACAA CAACCTCGGT 
CAGCGCTTAT TTGGGGAGAC CATCCTGGGG CTCACCCAAG GCTCTGTCTC TGACCTCCTT 
GCTCGCCCCA AGCCCTGGCA CAAGCTCAGT CTGAAGGGAC GGGAGCCCTT TGTCCGGATG 
CAGCTGTGGC TGAATGACCC CAACAACGTG GAGAAGCTGA TGGACATGAA ACGGATGGAA 
AAGAAAGCCT ACATGAAGCG GCGGCACAGC TCGGTCAGCG ACAGCCAGCC CTGTGAGCCC 
CCGTCCGTTG GCATCGACTA CAGCCAGGGC GCCAGCCCCC AGCCCCAGCA CCAGCTGAAG 
AAACCCCGAG TAGTGCTGGC CCCCGAAGAG AAAGAAGCTC TGAAACGAGC ATATCAGCAA 
AAGCCATACC CGTCACCAAA AACCATCGAA GAACTCGCCA CCCAGCTCAA CTTGAAAACC 
AGCACCGTCA TCAACTGGTT CCACAATTAC AGGTCTCGGA TCCGCAGAGA ACTGTTCATT 
GAGGAGATTC AGGCCGGGAG CCAGGGCCAG GCCGGGGCCA GCGATTCGCC GTCCGCCCGG 
AGCGGCCGGG CCGCACCCAG CTCAGAAGGC GACAGCTGCG ACGGCGTGGA GGCCGCCGAG 
GGCCCAGGCG CCGCGGACGC CGAAGAGTCG GGCGGCCCCG CGGCCGCCGC CAAGTCTCAG 
GGAGGGCCGG CCGAGGCGGC CGCGGCCCCA GAGGAGCGGG AGGAGGCGCC GCGGCCGGCG 
GAGAAGGCAG AGCCGCCGCC CTCGGGGACC CCGGTCCCGG ACGCCGCCGC CGACGAAGGC 
CGGCCCCGGG CGCCGCCGCC GCCGCCACCC GAGGGTCCCG CGGACGGCCC GGGGCCCGTG 
CCAAACCCCG CCGCCGCCGC ACCCGCGGCC GGGGAGGACG CCGCTACCTC AGCCGCCCCG 
CCCGGCGAGG GCCCCTGCCG GGCCCGGGAC GGCGCCGACA GGAGTTCCGC TTTGCCAAGC 
ACCAGCGCGC CCGCGGCTGC CCGCAGACCC AGCTCGCTGC AGAGCCTCTT CGGCCTCCCA 
GAGGCGGCGG GCGCCAGGGA CTCGCGAGAC AACCCCTTGA GGAAGAAGAA GGCCGCGAAC 
TTGAACAGCA TAATCCACCG CCTGGAGAAG GCTGCCAGCC GCGAGGAACC CATCGAATGG 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275399.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131184.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X3, mRNA.

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CloneID	OBu60244
Clone ID Related Accession (Same CDS sequence)	XM_025275399.1
Accession Version	XM_025275399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGC CATAGAGGTG 
CTGACCCGCT CCAGCCTGGA AGCTGAGTTG GCCGCCAAGG AGCGGGAGAT CGCCCAGCTG 
GTGGAGGACG TGCAGAGACT CCAGGCCAAC CTCAGCAAGC TGCGCGAGAA CTCAGCCAGC 
CAGATCTCCC AGCTCGAGCA GCAGCTGAGC GCCAAGAACA GCACCCTCAA GCAACTGGAA 
GAAAAACTCA AGGGACAGGC TGACTATGAA GAGGTGAAGA AAGAGCTAAA CATCCTGAAG 
TCCATGGAGT TTGCGCCGGC CGAGGGAGCT GGGACGCAGG ACGCATCCAG GCCCCTGGAG 
GTGCTGCTGC TGGAGAAGAA CCGCTCGCTG CAGTCCGAGA ACGCCGCGCT GCGCATCTCC 
AACAGCGACC TGAGCGGGTC AGCCAGGAGA AAAGGGAAAG ACCAGCCTGA GAGTCGGCGT 
CCTGGACCTT TGCCGGCCTC CCCTCCTTCT CAGTTGCCCC GCAACACGGG GGAGCAGGCT 
TCCAATACTA ATGGTACACA CCAGTTCTCA CCAGCGGGGT TAACGCAAGA CTTTTTCAGC 
TCATCCCTGG CAAGCCCCAG CCTGCCCCTG GCTTCTACGG GAAAATTTGC ACTAAACTCT 
CTCCTCCAAC GACAGCTAAT GCAGTCCTTC TACTCCAAGG CCATGCAGGA AGCAGGAAGC 
ACAAGCATGA TTTTTTCAAC AGGTCCATAC AGCACAAACT CCATATCTTC CCAAAGTCCA 
TTACAACAAA GCCCAGATGT AAATGGCATG GCCCCATCTC CCAGCCAGTC AGAAAGTGCT 
GGGAGCGTCT CCGAGGGCGA GGAGATAGAC ACTGCAGAAA TCGCCCGGCA GGTGAAAGAG 
CAGCTGATCA AGCACAATAT CGGACAGCGC ATTTTCGGAC ACTACGTCTT AGGACTGTCT 
CAGGGGTCCG TGAGCGAGAT CCTGGCCCGG CCCAAGCCGT GGAACAAACT GACGGTGCGC 
GGCAAGGAGC CGTTTCACAA GATGAAGCAG TTCCTCTCAG ACGAGCAGAA CATCCTGGCT 
CTTCGCAGCA TCCAAGGCAG ACAAAGAGAG AATCCAGGCC AGAGCCTGAA CAGACTCTTT 
CAGGAAGTAC CGAAACGAAG AAATGGGTCT GAAGGTAACA TCACCACCCG GATCCGAGCC 
TCCGAGACCG GGTCTGACGA AGCCATCAAG TCCATCTTGG AACAAGCCAA GAGGGAGCTG 
CAAGTCCAGA AAACCGCAGA GCCGGCCCAG CCTTCCTCGG CATCCAGTGG CGGGAGCGCA 
GACGACGCCA TCCGCTCCAT CCTGCAGCAG GCCCGCCGGG AGATGGAGGC CCAGCAGGCC 
GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG CCATCCCAGA CAGACATCGC CCTCCTGACC 
CCCAAGCTGA TACCCGCCTC GCCCATGTCG TCGGTGTCCA GTTACTCCCC TTTAGCCGTC 
TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC GCCAGCGCCC CTGCCCTGCC CAACCCGCCA 
GCCCTGAAGA AGGAGTCCCA GGATGCTCCC GGGCCGGACC TCCCAGGGGC GGCCGACCCC 
GCCGCACAGG GGGTCTTGAG ACACGTGAAG AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG 
GATCACTGGT GGAGCGCCGT CCAGCCCGAG AGGAAGAGCA GCGCTCCGCC CGAAGACCCC 
AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG 
CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC GAGTACTGGA AGGACTGGCC CAGCGCAGAG 
TCGCCCTACT CCCAGAGCTC AGAGCTGAGC CTGACCGGAG CCAGCCGCAG CGAGACGCCC 
CAGAACAGCC CACTGCCCTC CTCGCCCATT GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA 
GTCCCGCCAC TGACCCCCGA GCAGTACGAG ATCTACATGT ACCAGGAGGT GGACACCATC 
GAGCTCACCC GGCAGGTGAA GGAGAAGCTG GCCAAGAACG GCATCTGTCA GAGGATCTTC 
GGGGAGAAGG TGCTAGGCCT GTCACAGGGC AGTGTCAGTG ACATGCTGTC CAGGCCGAAG 
CCATGGAGCA AGCTGACCCA GAAAGGACGA GAACCCTTCA TACGGATGCA GCTCTGGCTG 
AACGGCGAGC TGGGCCAGGG CGTCCTGCCC GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG 
CACTCCGTGA CATCACTACA AGACCCCCTG CAGCAGGGCT GCGTGAGCTC AGAAAGCACT 
CCAAAGACCT CCGCCAGCTG CAGCCCTGCA CCCGAGTCAC CCATGAGCTC CAGTGAGTCA 
GTGAAGAGTC TGACTGAACT GGTCCAGCAG CCCTGCCCCC CCATCGAAAC CAGTAAGGAC 
AGCAAACCTC CAGAACCCAG CGACCCACCC GCCTCAGACT CCCAGCCCGC CACCCCACTG 
CCTCTCTCTG GACACTCAGC ACTCAGCATT CAAGAGCTGG TGGCCATGTC TCCAGAGCTG 
GACACCTACG GCATCACCAA GAGGGTCAAG GAGGTGTTGA CCGACAACAA CCTCGGTCAG 
CGCTTATTTG GGGAGACCAT CCTGGGGCTC ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT 
CGCCCCAAGC CCTGGCACAA GCTCAGTCTG AAGGGACGGG AGCCCTTTGT CCGGATGCAG 
CTGTGGCTGA ATGACCCCAA CAACGTGGAG AAGCTGATGG ACATGAAACG GATGGAAAAG 
AAAGCCTACA TGAAGCGGCG GCACAGCTCG GTCAGCGACA GCCAGCCCTG TGAGCCCCCG 
TCCGTTGGCA TCGACTACAG CCAGGGCGCC AGCCCCCAGC CCCAGCACCA GCTGAAGAAA 
CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA GAAGCTCTGA AACGAGCATA TCAGCAAAAG 
CCATACCCGT CACCAAAAAC CATCGAAGAA CTCGCCACCC AGCTCAACTT GAAAACCAGC 
ACCGTCATCA ACTGGTTCCA CAATTACAGG TCTCGGATCC GCAGAGAACT GTTCATTGAG 
GAGATTCAGG CCGGGAGCCA GGGCCAGGCC GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC 
GGCCGGGCCG CACCCAGCTC AGAAGGCGAC AGCTGCGACG GCGTGGAGGC CGCCGAGGGC 
CCAGGCGCCG CGGACGCCGA AGAGTCGGGC GGCCCCGCGG CCGCCGCCAA GTCTCAGGGA 
GGGCCGGCCG AGGCGGCCGC GGCCCCAGAG GAGCGGGAGG AGGCGCCGCG GCCGGCGGAG 
AAGGCAGAGC CGCCGCCCTC GGGGACCCCG GTCCCGGACG CCGCCGCCGA CGAAGGCCGG 
CCCCGGGCGC CGCCGCCGCC GCCACCCGAG GGTCCCGCGG ACGGCCCGGG GCCCGTGCCA 
AACCCCGCCG CCGCCGCACC CGCGGCCGGG GAGGACGCCG CTACCTCAGC CGCCCCGCCC 
GGCGAGGGCC CCTGCCGGGC CCGGGACGGC GCCGACAGGA GTTCCGCTTT GCCAAGCACC 
AGCGCGCCCG CGGCTGCCCG CAGACCCAGC TCGCTGCAGA GCCTCTTCGG CCTCCCAGAG 
GCGGCGGGCG CCAGGGACTC GCGAGACAAC CCCTTGAGGA AGAAGAAGGC CGCGAACTTG 
AACAGCATAA TCCACCGCCT GGAGAAGGCT GCCAGCCGCG AGGAACCCAT CGAATGGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131184.1
CDS	16..4881
Translation	MESAAGPLRNAYPDRVLESKAPGVPLDSSVPGRGPRTQPQAPRGDRAGAPHPSPEPHPCPGSAFLPATPHPGEQRALGVRLGPASSALAAPGQPRDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275399.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275399.1

ORF Insert Sequence:

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGC CATAGAGGTG 
CTGACCCGCT CCAGCCTGGA AGCTGAGTTG GCCGCCAAGG AGCGGGAGAT CGCCCAGCTG 
GTGGAGGACG TGCAGAGACT CCAGGCCAAC CTCAGCAAGC TGCGCGAGAA CTCAGCCAGC 
CAGATCTCCC AGCTCGAGCA GCAGCTGAGC GCCAAGAACA GCACCCTCAA GCAACTGGAA 
GAAAAACTCA AGGGACAGGC TGACTATGAA GAGGTGAAGA AAGAGCTAAA CATCCTGAAG 
TCCATGGAGT TTGCGCCGGC CGAGGGAGCT GGGACGCAGG ACGCATCCAG GCCCCTGGAG 
GTGCTGCTGC TGGAGAAGAA CCGCTCGCTG CAGTCCGAGA ACGCCGCGCT GCGCATCTCC 
AACAGCGACC TGAGCGGGTC AGCCAGGAGA AAAGGGAAAG ACCAGCCTGA GAGTCGGCGT 
CCTGGACCTT TGCCGGCCTC CCCTCCTTCT CAGTTGCCCC GCAACACGGG GGAGCAGGCT 
TCCAATACTA ATGGTACACA CCAGTTCTCA CCAGCGGGGT TAACGCAAGA CTTTTTCAGC 
TCATCCCTGG CAAGCCCCAG CCTGCCCCTG GCTTCTACGG GAAAATTTGC ACTAAACTCT 
CTCCTCCAAC GACAGCTAAT GCAGTCCTTC TACTCCAAGG CCATGCAGGA AGCAGGAAGC 
ACAAGCATGA TTTTTTCAAC AGGTCCATAC AGCACAAACT CCATATCTTC CCAAAGTCCA 
TTACAACAAA GCCCAGATGT AAATGGCATG GCCCCATCTC CCAGCCAGTC AGAAAGTGCT 
GGGAGCGTCT CCGAGGGCGA GGAGATAGAC ACTGCAGAAA TCGCCCGGCA GGTGAAAGAG 
CAGCTGATCA AGCACAATAT CGGACAGCGC ATTTTCGGAC ACTACGTCTT AGGACTGTCT 
CAGGGGTCCG TGAGCGAGAT CCTGGCCCGG CCCAAGCCGT GGAACAAACT GACGGTGCGC 
GGCAAGGAGC CGTTTCACAA GATGAAGCAG TTCCTCTCAG ACGAGCAGAA CATCCTGGCT 
CTTCGCAGCA TCCAAGGCAG ACAAAGAGAG AATCCAGGCC AGAGCCTGAA CAGACTCTTT 
CAGGAAGTAC CGAAACGAAG AAATGGGTCT GAAGGTAACA TCACCACCCG GATCCGAGCC 
TCCGAGACCG GGTCTGACGA AGCCATCAAG TCCATCTTGG AACAAGCCAA GAGGGAGCTG 
CAAGTCCAGA AAACCGCAGA GCCGGCCCAG CCTTCCTCGG CATCCAGTGG CGGGAGCGCA 
GACGACGCCA TCCGCTCCAT CCTGCAGCAG GCCCGCCGGG AGATGGAGGC CCAGCAGGCC 
GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG CCATCCCAGA CAGACATCGC CCTCCTGACC 
CCCAAGCTGA TACCCGCCTC GCCCATGTCG TCGGTGTCCA GTTACTCCCC TTTAGCCGTC 
TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC GCCAGCGCCC CTGCCCTGCC CAACCCGCCA 
GCCCTGAAGA AGGAGTCCCA GGATGCTCCC GGGCCGGACC TCCCAGGGGC GGCCGACCCC 
GCCGCACAGG GGGTCTTGAG ACACGTGAAG AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG 
GATCACTGGT GGAGCGCCGT CCAGCCCGAG AGGAAGAGCA GCGCTCCGCC CGAAGACCCC 
AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG 
CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC GAGTACTGGA AGGACTGGCC CAGCGCAGAG 
TCGCCCTACT CCCAGAGCTC AGAGCTGAGC CTGACCGGAG CCAGCCGCAG CGAGACGCCC 
CAGAACAGCC CACTGCCCTC CTCGCCCATT GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA 
GTCCCGCCAC TGACCCCCGA GCAGTACGAG ATCTACATGT ACCAGGAGGT GGACACCATC 
GAGCTCACCC GGCAGGTGAA GGAGAAGCTG GCCAAGAACG GCATCTGTCA GAGGATCTTC 
GGGGAGAAGG TGCTAGGCCT GTCACAGGGC AGTGTCAGTG ACATGCTGTC CAGGCCGAAG 
CCATGGAGCA AGCTGACCCA GAAAGGACGA GAACCCTTCA TACGGATGCA GCTCTGGCTG 
AACGGCGAGC TGGGCCAGGG CGTCCTGCCC GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG 
CACTCCGTGA CATCACTACA AGACCCCCTG CAGCAGGGCT GCGTGAGCTC AGAAAGCACT 
CCAAAGACCT CCGCCAGCTG CAGCCCTGCA CCCGAGTCAC CCATGAGCTC CAGTGAGTCA 
GTGAAGAGTC TGACTGAACT GGTCCAGCAG CCCTGCCCCC CCATCGAAAC CAGTAAGGAC 
AGCAAACCTC CAGAACCCAG CGACCCACCC GCCTCAGACT CCCAGCCCGC CACCCCACTG 
CCTCTCTCTG GACACTCAGC ACTCAGCATT CAAGAGCTGG TGGCCATGTC TCCAGAGCTG 
GACACCTACG GCATCACCAA GAGGGTCAAG GAGGTGTTGA CCGACAACAA CCTCGGTCAG 
CGCTTATTTG GGGAGACCAT CCTGGGGCTC ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT 
CGCCCCAAGC CCTGGCACAA GCTCAGTCTG AAGGGACGGG AGCCCTTTGT CCGGATGCAG 
CTGTGGCTGA ATGACCCCAA CAACGTGGAG AAGCTGATGG ACATGAAACG GATGGAAAAG 
AAAGCCTACA TGAAGCGGCG GCACAGCTCG GTCAGCGACA GCCAGCCCTG TGAGCCCCCG 
TCCGTTGGCA TCGACTACAG CCAGGGCGCC AGCCCCCAGC CCCAGCACCA GCTGAAGAAA 
CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA GAAGCTCTGA AACGAGCATA TCAGCAAAAG 
CCATACCCGT CACCAAAAAC CATCGAAGAA CTCGCCACCC AGCTCAACTT GAAAACCAGC 
ACCGTCATCA ACTGGTTCCA CAATTACAGG TCTCGGATCC GCAGAGAACT GTTCATTGAG 
GAGATTCAGG CCGGGAGCCA GGGCCAGGCC GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC 
GGCCGGGCCG CACCCAGCTC AGAAGGCGAC AGCTGCGACG GCGTGGAGGC CGCCGAGGGC 
CCAGGCGCCG CGGACGCCGA AGAGTCGGGC GGCCCCGCGG CCGCCGCCAA GTCTCAGGGA 
GGGCCGGCCG AGGCGGCCGC GGCCCCAGAG GAGCGGGAGG AGGCGCCGCG GCCGGCGGAG 
AAGGCAGAGC CGCCGCCCTC GGGGACCCCG GTCCCGGACG CCGCCGCCGA CGAAGGCCGG 
CCCCGGGCGC CGCCGCCGCC GCCACCCGAG GGTCCCGCGG ACGGCCCGGG GCCCGTGCCA 
AACCCCGCCG CCGCCGCACC CGCGGCCGGG GAGGACGCCG CTACCTCAGC CGCCCCGCCC 
GGCGAGGGCC CCTGCCGGGC CCGGGACGGC GCCGACAGGA GTTCCGCTTT GCCAAGCACC 
AGCGCGCCCG CGGCTGCCCG CAGACCCAGC TCGCTGCAGA GCCTCTTCGG CCTCCCAGAG 
GCGGCGGGCG CCAGGGACTC GCGAGACAAC CCCTTGAGGA AGAAGAAGGC CGCGAACTTG 
AACAGCATAA TCCACCGCCT GGAGAAGGCT GCCAGCCGCG AGGAACCCAT CGAATGGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275400.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131185.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X4, mRNA.

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CloneID	OBu60245
Clone ID Related Accession (Same CDS sequence)	XM_025275400.1
Accession Version	XM_025275400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGGTAACA TCACCACCCG GATCCGAGCC 
TCCGAGACCG GGTCTGACGA AGCCATCAAG TCCATCTTGG AACAAGCCAA GAGGGAGCTG 
CAAGTCCAGA AAACCGCAGA GCCGGCCCAG CCTTCCTCGG CATCCAGTGG CGGGAGCGCA 
GACGACGCCA TCCGCTCCAT CCTGCAGCAG GCCCGCCGGG AGATGGAGGC CCAGCAGGCC 
GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG CCATCCCAGA CAGACATCGC CCTCCTGACC 
CCCAAGCTGA TACCCGCCTC GCCCATGTCG TCGGTGTCCA GTTACTCCCC TTTAGCCGTC 
TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC GCCAGCGCCC CTGCCCTGCC CAACCCGCCA 
GCCCTGAAGA AGGAGTCCCA GGATGCTCCC GGGCCGGACC TCCCAGGGGC GGCCGACCCC 
GCCGCACAGG GGGTCTTGAG ACACGTGAAG AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG 
GATCACTGGT GGAGCGCCGT CCAGCCCGAG AGGAAGAGCA GCGCTCCGCC CGAAGACCCC 
AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG 
CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC GAGTACTGGA AGGACTGGCC CAGCGCAGAG 
TCGCCCTACT CCCAGAGCTC AGAGCTGAGC CTGACCGGAG CCAGCCGCAG CGAGACGCCC 
CAGAACAGCC CACTGCCCTC CTCGCCCATT GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA 
GTCCCGCCAC TGACCCCCGA GCAGTACGAG ATCTACATGT ACCAGGAGGT GGACACCATC 
GAGCTCACCC GGCAGGTGAA GGAGAAGCTG GCCAAGAACG GCATCTGTCA GAGGATCTTC 
GGGGAGAAGG TGCTAGGCCT GTCACAGGGC AGTGTCAGTG ACATGCTGTC CAGGCCGAAG 
CCATGGAGCA AGCTGACCCA GAAAGGACGA GAACCCTTCA TACGGATGCA GCTCTGGCTG 
AACGGCGAGC TGGGCCAGGG CGTCCTGCCC GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG 
CACTCCGTGA CATCACTACA AGACCCCCTG CAGCAGGGCT GCGTGAGCTC AGAAAGCACT 
CCAAAGACCT CCGCCAGCTG CAGCCCTGCA CCCGAGTCAC CCATGAGCTC CAGTGAGTCA 
GTGAAGAGTC TGACTGAACT GGTCCAGCAG CCCTGCCCCC CCATCGAAAC CAGTAAGGAC 
AGCAAACCTC CAGAACCCAG CGACCCACCC GCCTCAGACT CCCAGCCCGC CACCCCACTG 
CCTCTCTCTG GACACTCAGC ACTCAGCATT CAAGAGCTGG TGGCCATGTC TCCAGAGCTG 
GACACCTACG GCATCACCAA GAGGGTCAAG GAGGTGTTGA CCGACAACAA CCTCGGTCAG 
CGCTTATTTG GGGAGACCAT CCTGGGGCTC ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT 
CGCCCCAAGC CCTGGCACAA GCTCAGTCTG AAGGGACGGG AGCCCTTTGT CCGGATGCAG 
CTGTGGCTGA ATGACCCCAA CAACGTGGAG AAGCTGATGG ACATGAAACG GATGGAAAAG 
AAAGCCTACA TGAAGCGGCG GCACAGCTCG GTCAGCGACA GCCAGCCCTG TGAGCCCCCG 
TCCGTTGGCA TCGACTACAG CCAGGGCGCC AGCCCCCAGC CCCAGCACCA GCTGAAGAAA 
CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA GAAGCTCTGA AACGAGCATA TCAGCAAAAG 
CCATACCCGT CACCAAAAAC CATCGAAGAA CTCGCCACCC AGCTCAACTT GAAAACCAGC 
ACCGTCATCA ACTGGTTCCA CAATTACAGG TCTCGGATCC GCAGAGAACT GTTCATTGAG 
GAGATTCAGG CCGGGAGCCA GGGCCAGGCC GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC 
GGCCGGGCCG CACCCAGCTC AGAAGGCGAC AGCTGCGACG GCGTGGAGGC CGCCGAGGGC 
CCAGGCGCCG CGGACGCCGA AGAGTCGGGC GGCCCCGCGG CCGCCGCCAA GTCTCAGGGA 
GGGCCGGCCG AGGCGGCCGC GGCCCCAGAG GAGCGGGAGG AGGCGCCGCG GCCGGCGGAG 
AAGGCAGAGC CGCCGCCCTC GGGGACCCCG GTCCCGGACG CCGCCGCCGA CGAAGGCCGG 
CCCCGGGCGC CGCCGCCGCC GCCACCCGAG GGTCCCGCGG ACGGCCCGGG GCCCGTGCCA 
AACCCCGCCG CCGCCGCACC CGCGGCCGGG GAGGACGCCG CTACCTCAGC CGCCCCGCCC 
GGCGAGGGCC CCTGCCGGGC CCGGGACGGC GCCGACAGGA GTTCCGCTTT GCCAAGCACC 
AGCGCGCCCG CGGCTGCCCG CAGACCCAGC TCGCTGCAGA GCCTCTTCGG CCTCCCAGAG 
GCGGCGGGCG CCAGGGACTC GCGAGACAAC CCCTTGAGGA AGAAGAAGGC CGCGAACTTG 
AACAGCATAA TCCACCGCCT GGAGAAGGCT GCCAGCCGCG AGGAACCCAT CGAATGGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131185.1
CDS	16..4821
Translation	MESAAGPLRNAYPDRVLESKAPGVPLDSSVPGRGPRTQPQAPRGDRAGAPHPSPEPHPCPGSAFLPATPHPGEQRALGVRLGPASSALAAPGQPRDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275400.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275400.1

ORF Insert Sequence:

ATGGAGAGCG CGGCGGGTCC GCTCCGAAAT GCCTACCCCG ATCGTGTTCT CGAAAGCAAA 
GCCCCGGGTG TCCCACTGGA CAGCAGTGTG CCGGGCCGTG GTCCACGCAC CCAACCCCAG 
GCGCCGAGAG GGGATCGTGC TGGGGCTCCC CATCCGTCTC CCGAGCCGCA CCCGTGCCCA 
GGGAGCGCGT TCCTGCCTGC CACCCCCCAC CCAGGGGAGC AGCGCGCCCT CGGGGTCCGC 
CTGGGACCTG CCAGCTCCGC GCTGGCGGCT CCTGGACAGC CCCGGGACTC TGCCAGGTGG 
ATGTTGTGCG TAGCTGGAGC CAAGTTGAAG AGGGAACTCG ATGCCACCGC AACAGTATTG 
GCAAACCGGC AAGATGAAAG TGAGCAGTCA AGAAAGCGGC TCATCGAGCA GAGCCGTGAG 
TTCAAGAAGA ACACTCCAGA GGATTTGCGC AAGCAGGTAG CACCGCTGCT GAAGAGCTTC 
CAAGGCGAGA TTGATGCACT GAGTAAAAGA AGCAAAGAAG CTGAGGCAGC CTTCTTGAAT 
GTCTACAAGA GATTGATTGA TGTTCCAGAT CCCGTTCCCG CGCTGGACCT TGGGCAGCAG 
CTCCAGCTCA AAGTGCAGCG CCTGCACGAC ATTGAGACAG AGAACCAAAA ACTTAGGGAA 
ACGCTCGAAG AGTACAACAA GGAATTCGCT GAAGTGAAAA ATCAAGAGGT TACGATAAAA 
GCACTTAAAG AGAAAATCCG AGAATATGAA CAGACTCTGA AGAACCAGGC TGAGACCATA 
GCTCTCGAAA AGGAACAAAA GTTACAAAAT GATTTTGCAG AAAAGGAGAG AAAGCTGCAG 
GAGACCCAGA TGTCCACCAC CTCAAAGCTA GAAGAAGCCG AACATAAAGT CCAGACTCTG 
CAAACAGCCC TGGAAAAAAC TCGAACAGAA TTATTTGACC TGAAAACCAA ATATGATGAA 
GAAATTACTG CCAAGGCCGA CGAAATTGAA ATGATCATGA CGGACCTTGA AAGAGCAAAC 
CAGAGGGCAG AGGTGGCTCA AAGAGAGGCG GAGACCTTGA GGGAGCAGCT GTCGTCGGCC 
AACCACTCTC TCCAGCTGGC CTCCCAGATC CAGAAGGCAC CGGACGTGGA GCAGGCCATA 
GAGGTGCTGA CCCGCTCCAG CCTGGAAGCT GAGTTGGCCG CCAAGGAGCG GGAGATCGCC 
CAGCTGGTGG AGGACGTGCA GAGACTCCAG GCCAACCTCA GCAAGCTGCG CGAGAACTCA 
GCCAGCCAGA TCTCCCAGCT CGAGCAGCAG CTGAGCGCCA AGAACAGCAC CCTCAAGCAA 
CTGGAAGAAA AACTCAAGGG ACAGGCTGAC TATGAAGAGG TGAAGAAAGA GCTAAACATC 
CTGAAGTCCA TGGAGTTTGC GCCGGCCGAG GGAGCTGGGA CGCAGGACGC ATCCAGGCCC 
CTGGAGGTGC TGCTGCTGGA GAAGAACCGC TCGCTGCAGT CCGAGAACGC CGCGCTGCGC 
ATCTCCAACA GCGACCTGAG CGGGTCAGCC AGGAGAAAAG GGAAAGACCA GCCTGAGAGT 
CGGCGTCCTG GACCTTTGCC GGCCTCCCCT CCTTCTCAGT TGCCCCGCAA CACGGGGGAG 
CAGGCTTCCA ATACTAATGG TACACACCAG TTCTCACCAG CGGGGTTAAC GCAAGACTTT 
TTCAGCTCAT CCCTGGCAAG CCCCAGCCTG CCCCTGGCTT CTACGGGAAA ATTTGCACTA 
AACTCTCTCC TCCAACGACA GCTAATGCAG TCCTTCTACT CCAAGGCCAT GCAGGAAGCA 
GGAAGCACAA GCATGATTTT TTCAACAGGT CCATACAGCA CAAACTCCAT ATCTTCCCAA 
AGTCCATTAC AACAAAGCCC AGATGTAAAT GGCATGGCCC CATCTCCCAG CCAGTCAGAA 
AGTGCTGGGA GCGTCTCCGA GGGCGAGGAG ATAGACACTG CAGAAATCGC CCGGCAGGTG 
AAAGAGCAGC TGATCAAGCA CAATATCGGA CAGCGCATTT TCGGACACTA CGTCTTAGGA 
CTGTCTCAGG GGTCCGTGAG CGAGATCCTG GCCCGGCCCA AGCCGTGGAA CAAACTGACG 
GTGCGCGGCA AGGAGCCGTT TCACAAGATG AAGCAGTTCC TCTCAGACGA GCAGAACATC 
CTGGCTCTTC GCAGCATCCA AGGCAGACAA AGAGGTAACA TCACCACCCG GATCCGAGCC 
TCCGAGACCG GGTCTGACGA AGCCATCAAG TCCATCTTGG AACAAGCCAA GAGGGAGCTG 
CAAGTCCAGA AAACCGCAGA GCCGGCCCAG CCTTCCTCGG CATCCAGTGG CGGGAGCGCA 
GACGACGCCA TCCGCTCCAT CCTGCAGCAG GCCCGCCGGG AGATGGAGGC CCAGCAGGCC 
GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG CCATCCCAGA CAGACATCGC CCTCCTGACC 
CCCAAGCTGA TACCCGCCTC GCCCATGTCG TCGGTGTCCA GTTACTCCCC TTTAGCCGTC 
TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC GCCAGCGCCC CTGCCCTGCC CAACCCGCCA 
GCCCTGAAGA AGGAGTCCCA GGATGCTCCC GGGCCGGACC TCCCAGGGGC GGCCGACCCC 
GCCGCACAGG GGGTCTTGAG ACACGTGAAG AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG 
GATCACTGGT GGAGCGCCGT CCAGCCCGAG AGGAAGAGCA GCGCTCCGCC CGAAGACCCC 
AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG 
CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC GAGTACTGGA AGGACTGGCC CAGCGCAGAG 
TCGCCCTACT CCCAGAGCTC AGAGCTGAGC CTGACCGGAG CCAGCCGCAG CGAGACGCCC 
CAGAACAGCC CACTGCCCTC CTCGCCCATT GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA 
GTCCCGCCAC TGACCCCCGA GCAGTACGAG ATCTACATGT ACCAGGAGGT GGACACCATC 
GAGCTCACCC GGCAGGTGAA GGAGAAGCTG GCCAAGAACG GCATCTGTCA GAGGATCTTC 
GGGGAGAAGG TGCTAGGCCT GTCACAGGGC AGTGTCAGTG ACATGCTGTC CAGGCCGAAG 
CCATGGAGCA AGCTGACCCA GAAAGGACGA GAACCCTTCA TACGGATGCA GCTCTGGCTG 
AACGGCGAGC TGGGCCAGGG CGTCCTGCCC GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG 
CACTCCGTGA CATCACTACA AGACCCCCTG CAGCAGGGCT GCGTGAGCTC AGAAAGCACT 
CCAAAGACCT CCGCCAGCTG CAGCCCTGCA CCCGAGTCAC CCATGAGCTC CAGTGAGTCA 
GTGAAGAGTC TGACTGAACT GGTCCAGCAG CCCTGCCCCC CCATCGAAAC CAGTAAGGAC 
AGCAAACCTC CAGAACCCAG CGACCCACCC GCCTCAGACT CCCAGCCCGC CACCCCACTG 
CCTCTCTCTG GACACTCAGC ACTCAGCATT CAAGAGCTGG TGGCCATGTC TCCAGAGCTG 
GACACCTACG GCATCACCAA GAGGGTCAAG GAGGTGTTGA CCGACAACAA CCTCGGTCAG 
CGCTTATTTG GGGAGACCAT CCTGGGGCTC ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT 
CGCCCCAAGC CCTGGCACAA GCTCAGTCTG AAGGGACGGG AGCCCTTTGT CCGGATGCAG 
CTGTGGCTGA ATGACCCCAA CAACGTGGAG AAGCTGATGG ACATGAAACG GATGGAAAAG 
AAAGCCTACA TGAAGCGGCG GCACAGCTCG GTCAGCGACA GCCAGCCCTG TGAGCCCCCG 
TCCGTTGGCA TCGACTACAG CCAGGGCGCC AGCCCCCAGC CCCAGCACCA GCTGAAGAAA 
CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA GAAGCTCTGA AACGAGCATA TCAGCAAAAG 
CCATACCCGT CACCAAAAAC CATCGAAGAA CTCGCCACCC AGCTCAACTT GAAAACCAGC 
ACCGTCATCA ACTGGTTCCA CAATTACAGG TCTCGGATCC GCAGAGAACT GTTCATTGAG 
GAGATTCAGG CCGGGAGCCA GGGCCAGGCC GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC 
GGCCGGGCCG CACCCAGCTC AGAAGGCGAC AGCTGCGACG GCGTGGAGGC CGCCGAGGGC 
CCAGGCGCCG CGGACGCCGA AGAGTCGGGC GGCCCCGCGG CCGCCGCCAA GTCTCAGGGA 
GGGCCGGCCG AGGCGGCCGC GGCCCCAGAG GAGCGGGAGG AGGCGCCGCG GCCGGCGGAG 
AAGGCAGAGC CGCCGCCCTC GGGGACCCCG GTCCCGGACG CCGCCGCCGA CGAAGGCCGG 
CCCCGGGCGC CGCCGCCGCC GCCACCCGAG GGTCCCGCGG ACGGCCCGGG GCCCGTGCCA 
AACCCCGCCG CCGCCGCACC CGCGGCCGGG GAGGACGCCG CTACCTCAGC CGCCCCGCCC 
GGCGAGGGCC CCTGCCGGGC CCGGGACGGC GCCGACAGGA GTTCCGCTTT GCCAAGCACC 
AGCGCGCCCG CGGCTGCCCG CAGACCCAGC TCGCTGCAGA GCCTCTTCGG CCTCCCAGAG 
GCGGCGGGCG CCAGGGACTC GCGAGACAAC CCCTTGAGGA AGAAGAAGGC CGCGAACTTG 
AACAGCATAA TCCACCGCCT GGAGAAGGCT GCCAGCCGCG AGGAACCCAT CGAATGGGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_025275401.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131186.1 Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X5, mRNA.

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CloneID	OBu60246
Clone ID Related Accession (Same CDS sequence)	XM_025275401.1
Accession Version	XM_025275401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein CASP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGGTCA 
GCCAGGAGAA AAGGGAAAGA CCAGCCTGAG AGTCGGCGTC CTGGACCTTT GCCGGCCTCC 
CCTCCTTCTC AGTTGCCCCG CAACACGGGG GAGCAGGCTT CCAATACTAA TGGTACACAC 
CAGTTCTCAC CAGCGGGGTT AACGCAAGAC TTTTTCAGCT CATCCCTGGC AAGCCCCAGC 
CTGCCCCTGG CTTCTACGGG AAAATTTGCA CTAAACTCTC TCCTCCAACG ACAGCTAATG 
CAGTCCTTCT ACTCCAAGGC CATGCAGGAA GCAGGAAGCA CAAGCATGAT TTTTTCAACA 
GGTCCATACA GCACAAACTC CATATCTTCC CAAAGTCCAT TACAACAAAG CCCAGATGTA 
AATGGCATGG CCCCATCTCC CAGCCAGTCA GAAAGTGCTG GGAGCGTCTC CGAGGGCGAG 
GAGATAGACA CTGCAGAAAT CGCCCGGCAG GTGAAAGAGC AGCTGATCAA GCACAATATC 
GGACAGCGCA TTTTCGGACA CTACGTCTTA GGACTGTCTC AGGGGTCCGT GAGCGAGATC 
CTGGCCCGGC CCAAGCCGTG GAACAAACTG ACGGTGCGCG GCAAGGAGCC GTTTCACAAG 
ATGAAGCAGT TCCTCTCAGA CGAGCAGAAC ATCCTGGCTC TTCGCAGCAT CCAAGGCAGA 
CAAAGAGAGA ATCCAGGCCA GAGCCTGAAC AGACTCTTTC AGGAAGTACC GAAACGAAGA 
AATGGGTCTG AAGGTAACAT CACCACCCGG ATCCGAGCCT CCGAGACCGG GTCTGACGAA 
GCCATCAAGT CCATCTTGGA ACAAGCCAAG AGGGAGCTGC AAGTCCAGAA AACCGCAGAG 
CCGGCCCAGC CTTCCTCGGC ATCCAGTGGC GGGAGCGCAG ACGACGCCAT CCGCTCCATC 
CTGCAGCAGG CCCGCCGGGA GATGGAGGCC CAGCAGGCCG CCCTCGAGCC TGCCTTAAAG 
CCCGCGCCGC CATCCCAGAC AGACATCGCC CTCCTGACCC CCAAGCTGAT ACCCGCCTCG 
CCCATGTCGT CGGTGTCCAG TTACTCCCCT TTAGCCGTCT CCCTGAAGAA GCCCCCCTCG 
GCTCCCGACG CCAGCGCCCC TGCCCTGCCC AACCCGCCAG CCCTGAAGAA GGAGTCCCAG 
GATGCTCCCG GGCCGGACCT CCCAGGGGCG GCCGACCCCG CCGCACAGGG GGTCTTGAGA 
CACGTGAAGA GTGAGCTCGG CCGCGGCACC GTGTGGAAGG ATCACTGGTG GAGCGCCGTC 
CAGCCCGAGA GGAAGAGCAG CGCTCCGCCC GAAGACCCCA AGGCCGAAGA CGCCAGCAGC 
AGCAAAGAAA AAGGCAGCGG CGGGCAGGCG CGGGCGGAGC GCAGCCAGCT CCAGGGCCCC 
TCCTCCTCCG AGTACTGGAA GGACTGGCCC AGCGCAGAGT CGCCCTACTC CCAGAGCTCA 
GAGCTGAGCC TGACCGGAGC CAGCCGCAGC GAGACGCCCC AGAACAGCCC ACTGCCCTCC 
TCGCCCATTG TGCCCCTGTC GAAGCCTACC AAGCCCTCAG TCCCGCCACT GACCCCCGAG 
CAGTACGAGA TCTACATGTA CCAGGAGGTG GACACCATCG AGCTCACCCG GCAGGTGAAG 
GAGAAGCTGG CCAAGAACGG CATCTGTCAG AGGATCTTCG GGGAGAAGGT GCTAGGCCTG 
TCACAGGGCA GTGTCAGTGA CATGCTGTCC AGGCCGAAGC CATGGAGCAA GCTGACCCAG 
AAAGGACGAG AACCCTTCAT ACGGATGCAG CTCTGGCTGA ACGGCGAGCT GGGCCAGGGC 
GTCCTGCCCG TCCAAGGGCA GCAGCAAGGG CCAGTCTTGC ACTCCGTGAC ATCACTACAA 
GACCCCCTGC AGCAGGGCTG CGTGAGCTCA GAAAGCACTC CAAAGACCTC CGCCAGCTGC 
AGCCCTGCAC CCGAGTCACC CATGAGCTCC AGTGAGTCAG TGAAGAGTCT GACTGAACTG 
GTCCAGCAGC CCTGCCCCCC CATCGAAACC AGTAAGGACA GCAAACCTCC AGAACCCAGC 
GACCCACCCG CCTCAGACTC CCAGCCCGCC ACCCCACTGC CTCTCTCTGG ACACTCAGCA 
CTCAGCATTC AAGAGCTGGT GGCCATGTCT CCAGAGCTGG ACACCTACGG CATCACCAAG 
AGGGTCAAGG AGGTGTTGAC CGACAACAAC CTCGGTCAGC GCTTATTTGG GGAGACCATC 
CTGGGGCTCA CCCAAGGCTC TGTCTCTGAC CTCCTTGCTC GCCCCAAGCC CTGGCACAAG 
CTCAGTCTGA AGGGACGGGA GCCCTTTGTC CGGATGCAGC TGTGGCTGAA TGACCCCAAC 
AACGTGGAGA AGCTGATGGA CATGAAACGG ATGGAAAAGA AAGCCTACAT GAAGCGGCGG 
CACAGCTCGG TCAGCGACAG CCAGCCCTGT GAGCCCCCGT CCGTTGGCAT CGACTACAGC 
CAGGGCGCCA GCCCCCAGCC CCAGCACCAG CTGAAGAAAC CCCGAGTAGT GCTGGCCCCC 
GAAGAGAAAG AAGCTCTGAA ACGAGCATAT CAGCAAAAGC CATACCCGTC ACCAAAAACC 
ATCGAAGAAC TCGCCACCCA GCTCAACTTG AAAACCAGCA CCGTCATCAA CTGGTTCCAC 
AATTACAGGT CTCGGATCCG CAGAGAACTG TTCATTGAGG AGATTCAGGC CGGGAGCCAG 
GGCCAGGCCG GGGCCAGCGA TTCGCCGTCC GCCCGGAGCG GCCGGGCCGC ACCCAGCTCA 
GAAGGCGACA GCTGCGACGG CGTGGAGGCC GCCGAGGGCC CAGGCGCCGC GGACGCCGAA 
GAGTCGGGCG GCCCCGCGGC CGCCGCCAAG TCTCAGGGAG GGCCGGCCGA GGCGGCCGCG 
GCCCCAGAGG AGCGGGAGGA GGCGCCGCGG CCGGCGGAGA AGGCAGAGCC GCCGCCCTCG 
GGGACCCCGG TCCCGGACGC CGCCGCCGAC GAAGGCCGGC CCCGGGCGCC GCCGCCGCCG 
CCACCCGAGG GTCCCGCGGA CGGCCCGGGG CCCGTGCCAA ACCCCGCCGC CGCCGCACCC 
GCGGCCGGGG AGGACGCCGC TACCTCAGCC GCCCCGCCCG GCGAGGGCCC CTGCCGGGCC 
CGGGACGGCG CCGACAGGAG TTCCGCTTTG CCAAGCACCA GCGCGCCCGC GGCTGCCCGC 
AGACCCAGCT CGCTGCAGAG CCTCTTCGGC CTCCCAGAGG CGGCGGGCGC CAGGGACTCG 
CGAGACAACC CCTTGAGGAA GAAGAAGGCC GCGAACTTGA ACAGCATAAT CCACCGCCTG 
GAGAAGGCTG CCAGCCGCGA GGAACCCATC GAATGGGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131186.1
CDS	166..4770
Translation	MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAADAEESGGPAAAAKSQGGPAEAAAAPEEREEAPRPAEKAEPPPSGTPVPDAAADEGRPRAPPPPPPEGPADGPGPVPNPAAAAPAAGEDAATSAAPPGEGPCRARDGADRSSALPSTSAPAAARRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF

Target ORF information:

RefSeq Version	XM_025275401.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut like homeobox 1 (CUX1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025275401.1

ORF Insert Sequence:

ATGGCGGCCA ATGTGGGATC GATGTTTCAA TATTGGAAGC GCTTTGATTT ACAGCAGCTG 
CAGAGGGAAC TCGATGCCAC CGCAACAGTA TTGGCAAACC GGCAAGATGA AAGTGAGCAG 
TCAAGAAAGC GGCTCATCGA GCAGAGCCGT GAGTTCAAGA AGAACACTCC AGAGGATTTG 
CGCAAGCAGG TAGCACCGCT GCTGAAGAGC TTCCAAGGCG AGATTGATGC ACTGAGTAAA 
AGAAGCAAAG AAGCTGAGGC AGCCTTCTTG AATGTCTACA AGAGATTGAT TGATGTTCCA 
GATCCCGTTC CCGCGCTGGA CCTTGGGCAG CAGCTCCAGC TCAAAGTGCA GCGCCTGCAC 
GACATTGAGA CAGAGAACCA AAAACTTAGG GAAACGCTCG AAGAGTACAA CAAGGAATTC 
GCTGAAGTGA AAAATCAAGA GGTTACGATA AAAGCACTTA AAGAGAAAAT CCGAGAATAT 
GAACAGACTC TGAAGAACCA GGCTGAGACC ATAGCTCTCG AAAAGGAACA AAAGTTACAA 
AATGATTTTG CAGAAAAGGA GAGAAAGCTG CAGGAGACCC AGATGTCCAC CACCTCAAAG 
CTAGAAGAAG CCGAACATAA AGTCCAGACT CTGCAAACAG CCCTGGAAAA AACTCGAACA 
GAATTATTTG ACCTGAAAAC CAAATATGAT GAAGAAATTA CTGCCAAGGC CGACGAAATT 
GAAATGATCA TGACGGACCT TGAAAGAGCA AACCAGAGGG CAGAGGTGGC TCAAAGAGAG 
GCGGAGACCT TGAGGGAGCA GCTGTCGTCG GCCAACCACT CTCTCCAGCT GGCCTCCCAG 
ATCCAGAAGG CACCGGACGT GGAGCAGGCC ATAGAGGTGC TGACCCGCTC CAGCCTGGAA 
GCTGAGTTGG CCGCCAAGGA GCGGGAGATC GCCCAGCTGG TGGAGGACGT GCAGAGACTC 
CAGGCCAACC TCAGCAAGCT GCGCGAGAAC TCAGCCAGCC AGATCTCCCA GCTCGAGCAG 
CAGCTGAGCG CCAAGAACAG CACCCTCAAG CAACTGGAAG AAAAACTCAA GGGACAGGCT 
GACTATGAAG AGGTGAAGAA AGAGCTAAAC ATCCTGAAGT CCATGGAGTT TGCGCCGGCC 
GAGGGAGCTG GGACGCAGGA CGCATCCAGG CCCCTGGAGG TGCTGCTGCT GGAGAAGAAC 
CGCTCGCTGC AGTCCGAGAA CGCCGCGCTG CGCATCTCCA ACAGCGACCT GAGCGGGTCA 
GCCAGGAGAA AAGGGAAAGA CCAGCCTGAG AGTCGGCGTC CTGGACCTTT GCCGGCCTCC 
CCTCCTTCTC AGTTGCCCCG CAACACGGGG GAGCAGGCTT CCAATACTAA TGGTACACAC 
CAGTTCTCAC CAGCGGGGTT AACGCAAGAC TTTTTCAGCT CATCCCTGGC AAGCCCCAGC 
CTGCCCCTGG CTTCTACGGG AAAATTTGCA CTAAACTCTC TCCTCCAACG ACAGCTAATG 
CAGTCCTTCT ACTCCAAGGC CATGCAGGAA GCAGGAAGCA CAAGCATGAT TTTTTCAACA 
GGTCCATACA GCACAAACTC CATATCTTCC CAAAGTCCAT TACAACAAAG CCCAGATGTA 
AATGGCATGG CCCCATCTCC CAGCCAGTCA GAAAGTGCTG GGAGCGTCTC CGAGGGCGAG 
GAGATAGACA CTGCAGAAAT CGCCCGGCAG GTGAAAGAGC AGCTGATCAA GCACAATATC 
GGACAGCGCA TTTTCGGACA CTACGTCTTA GGACTGTCTC AGGGGTCCGT GAGCGAGATC 
CTGGCCCGGC CCAAGCCGTG GAACAAACTG ACGGTGCGCG GCAAGGAGCC GTTTCACAAG 
ATGAAGCAGT TCCTCTCAGA CGAGCAGAAC ATCCTGGCTC TTCGCAGCAT CCAAGGCAGA 
CAAAGAGAGA ATCCAGGCCA GAGCCTGAAC AGACTCTTTC AGGAAGTACC GAAACGAAGA 
AATGGGTCTG AAGGTAACAT CACCACCCGG ATCCGAGCCT CCGAGACCGG GTCTGACGAA 
GCCATCAAGT CCATCTTGGA ACAAGCCAAG AGGGAGCTGC AAGTCCAGAA AACCGCAGAG 
CCGGCCCAGC CTTCCTCGGC ATCCAGTGGC GGGAGCGCAG ACGACGCCAT CCGCTCCATC 
CTGCAGCAGG CCCGCCGGGA GATGGAGGCC CAGCAGGCCG CCCTCGAGCC TGCCTTAAAG 
CCCGCGCCGC CATCCCAGAC AGACATCGCC CTCCTGACCC CCAAGCTGAT ACCCGCCTCG 
CCCATGTCGT CGGTGTCCAG TTACTCCCCT TTAGCCGTCT CCCTGAAGAA GCCCCCCTCG 
GCTCCCGACG CCAGCGCCCC TGCCCTGCCC AACCCGCCAG CCCTGAAGAA GGAGTCCCAG 
GATGCTCCCG GGCCGGACCT CCCAGGGGCG GCCGACCCCG CCGCACAGGG GGTCTTGAGA 
CACGTGAAGA GTGAGCTCGG CCGCGGCACC GTGTGGAAGG ATCACTGGTG GAGCGCCGTC 
CAGCCCGAGA GGAAGAGCAG CGCTCCGCCC GAAGACCCCA AGGCCGAAGA CGCCAGCAGC 
AGCAAAGAAA AAGGCAGCGG CGGGCAGGCG CGGGCGGAGC GCAGCCAGCT CCAGGGCCCC 
TCCTCCTCCG AGTACTGGAA GGACTGGCCC AGCGCAGAGT CGCCCTACTC CCAGAGCTCA 
GAGCTGAGCC TGACCGGAGC CAGCCGCAGC GAGACGCCCC AGAACAGCCC ACTGCCCTCC 
TCGCCCATTG TGCCCCTGTC GAAGCCTACC AAGCCCTCAG TCCCGCCACT GACCCCCGAG 
CAGTACGAGA TCTACATGTA CCAGGAGGTG GACACCATCG AGCTCACCCG GCAGGTGAAG 
GAGAAGCTGG CCAAGAACGG CATCTGTCAG AGGATCTTCG GGGAGAAGGT GCTAGGCCTG 
TCACAGGGCA GTGTCAGTGA CATGCTGTCC AGGCCGAAGC CATGGAGCAA GCTGACCCAG 
AAAGGACGAG AACCCTTCAT ACGGATGCAG CTCTGGCTGA ACGGCGAGCT GGGCCAGGGC 
GTCCTGCCCG TCCAAGGGCA GCAGCAAGGG CCAGTCTTGC ACTCCGTGAC ATCACTACAA 
GACCCCCTGC AGCAGGGCTG CGTGAGCTCA GAAAGCACTC CAAAGACCTC CGCCAGCTGC 
AGCCCTGCAC CCGAGTCACC CATGAGCTCC AGTGAGTCAG TGAAGAGTCT GACTGAACTG 
GTCCAGCAGC CCTGCCCCCC CATCGAAACC AGTAAGGACA GCAAACCTCC AGAACCCAGC 
GACCCACCCG CCTCAGACTC CCAGCCCGCC ACCCCACTGC CTCTCTCTGG ACACTCAGCA 
CTCAGCATTC AAGAGCTGGT GGCCATGTCT CCAGAGCTGG ACACCTACGG CATCACCAAG 
AGGGTCAAGG AGGTGTTGAC CGACAACAAC CTCGGTCAGC GCTTATTTGG GGAGACCATC 
CTGGGGCTCA CCCAAGGCTC TGTCTCTGAC CTCCTTGCTC GCCCCAAGCC CTGGCACAAG 
CTCAGTCTGA AGGGACGGGA GCCCTTTGTC CGGATGCAGC TGTGGCTGAA TGACCCCAAC 
AACGTGGAGA AGCTGATGGA CATGAAACGG ATGGAAAAGA AAGCCTACAT GAAGCGGCGG 
CACAGCTCGG TCAGCGACAG CCAGCCCTGT GAGCCCCCGT CCGTTGGCAT CGACTACAGC 
CAGGGCGCCA GCCCCCAGCC CCAGCACCAG CTGAAGAAAC CCCGAGTAGT GCTGGCCCCC 
GAAGAGAAAG AAGCTCTGAA ACGAGCATAT CAGCAAAAGC CATACCCGTC ACCAAAAACC 
ATCGAAGAAC TCGCCACCCA GCTCAACTTG AAAACCAGCA CCGTCATCAA CTGGTTCCAC 
AATTACAGGT CTCGGATCCG CAGAGAACTG TTCATTGAGG AGATTCAGGC CGGGAGCCAG 
GGCCAGGCCG GGGCCAGCGA TTCGCCGTCC GCCCGGAGCG GCCGGGCCGC ACCCAGCTCA 
GAAGGCGACA GCTGCGACGG CGTGGAGGCC GCCGAGGGCC CAGGCGCCGC GGACGCCGAA 
GAGTCGGGCG GCCCCGCGGC CGCCGCCAAG TCTCAGGGAG GGCCGGCCGA GGCGGCCGCG 
GCCCCAGAGG AGCGGGAGGA GGCGCCGCGG CCGGCGGAGA AGGCAGAGCC GCCGCCCTCG 
GGGACCCCGG TCCCGGACGC CGCCGCCGAC GAAGGCCGGC CCCGGGCGCC GCCGCCGCCG 
CCACCCGAGG GTCCCGCGGA CGGCCCGGGG CCCGTGCCAA ACCCCGCCGC CGCCGCACCC 
GCGGCCGGGG AGGACGCCGC TACCTCAGCC GCCCCGCCCG GCGAGGGCCC CTGCCGGGCC 
CGGGACGGCG CCGACAGGAG TTCCGCTTTG CCAAGCACCA GCGCGCCCGC GGCTGCCCGC 
AGACCCAGCT CGCTGCAGAG CCTCTTCGGC CTCCCAGAGG CGGCGGGCGC CAGGGACTCG 
CGAGACAACC CCTTGAGGAA GAAGAAGGCC GCGAACTTGA ACAGCATAAT CCACCGCCTG 
GAGAAGGCTG CCAGCCGCGA GGAACCCATC GAATGGGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUX1 ( XM_006059466.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059528.1 Bubalus bubalis cut-like homeobox 1 (CUX1), mRNA.

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CloneID	OBu16058
Clone ID Related Accession (Same CDS sequence)	XM_006059466.1
Accession Version	XM_006059466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	homeobox protein cut-like 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784913.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTACC TTCAAACCAG GACCTCCGTG TATCTCAAAT CTGTGTCCCA AGCACCGTCC 
TCAGCCTTCA TCACCTTTGG GCCAGGTTCA GGCATCACCG ATCTGGATTT GCGCAAGCAG 
GTAGCACCGC TGCTGAAGAG CTTCCAAGGC GAGATTGATG CACTGAGTAA AAGAAGCAAA 
GAAGCTGAGG CAGCCTTCTT GAATGTCTAC AAGAGATTGA TTGATGTTCC AGATCCCGTT 
CCCGCGCTGG ACCTTGGGCA GCAGCTCCAG CTCAAAGTGC AGCGCCTGCA CGACATTGAG 
ACAGAGAACC AAAAACTTAG GGAAACGCTC GAAGAGTACA ACAAGGAATT CGCTGAAGTG 
AAAAATCAAG AGGTTACGAT AAAAGCACTT AAAGAGAAAA TCCGAGAATA TGAACAGACT 
CTGAAGAACC AGGCTGAGAC CATAGCTCTC GAAAAGGAAC AAAAGTTACA AAATGATTTT 
GCAGAAAAGG AGAGAAAGCT GCAGGAGACC CAGATGTCCA CCACCTCAAA GCTAGAAGAA 
GCCGAACATA AAGTCCAGAC TCTGCAAACA GCCCTGGAAA AAACTCGAAC AGAATTATTT 
GACCTGAAAA CCAAATATGA TGAAGAAATT ACTGCCAAGG CCGACGAAAT TGAAATGATC 
ATGACGGACC TTGAAAGAGC AAACCAGAGG GCAGAGGTGG CTCAAAGAGA GGCGGAGACC 
TTGAGGGAGC AGCTGTCGTC GGCCAACCAC TCTCTCCAGC TGGCCTCCCA GATCCAGAAG 
GCACCGGACG TGGAGCAGGC CATAGAGGTG CTGACCCGCT CCAGCCTGGA AGCTGAGTTG 
GCCGCCAAGG AGCGGGAGAT CGCCCAGCTG GTGGAGGACG TGCAGAGACT CCAGGCCAAC 
CTCAGCAAGC TGCGCGAGAA CTCAGCCAGC CAGATCTCCC AGCTCGAGCA GCAGCTGAGC 
GCCAAGAACA GCACCCTCAA GCAACTGGAA GAAAAACTCA AGGGACAGGC TGACTATGAA 
GAGGTGAAGA AAGAGCTAAA CATCCTGAAG TCCATGGAGT TTGCGCCGGC CGAGGGAGCT 
GGGACGCAGG ACGCATCCAG GCCCCTGGAG GTGCTGCTGC TGGAGAAGAA CCGCTCGCTG 
CAGTCCGAGA ACGCCGCGCT GCGCATCTCC AACAGCGACC TGAGCGGGTC AGCCAGGAGA 
AAAGGGAAAG ACCAGCCTGA GAGTCGGCGT CCTGGACCTT TGCCGGCCTC CCCTCCTTCT 
CAGTTGCCCC GCAACACGGG GGAGCAGGCT TCCAATACTA ATGGTACACA CCAGTTCTCA 
CCAGCGGGGT TAACGCAAGA CTTTTTCAGC TCATCCCTGG CAAGCCCCAG CCTGCCCCTG 
GCTTCTACGG GAAAATTTGC ACTAAACTCT CTCCTCCAAC GACAGCTAAT GCAGTCCTTC 
TACTCCAAGG CCATGCAGGA AGCAGGAAGC ACAAGCATGA TTTTTTCAAC AGGTCCATAC 
AGCACAAACT CCATATCTTC CCAAAGTCCA TTACAACAAA GCCCAGATGT AAATGGCATG 
GCCCCATCTC CCAGCCAGTC AGAAAGTGCT GGGAGCGTCT CCGAGGGCGA GGAGATAGAC 
ACTGCAGAAA TCGCCCGGCA GGTGAAAGAG CAGCTGATCA AGCACAATAT CGGACAGCGC 
ATTTTCGGAC ACTACGTCTT AGGACTGTCT CAGGGGTCCG TGAGCGAGAT CCTGGCCCGG 
CCCAAGCCGT GGAACAAACT GACGGTGCGC GGCAAGGAGC CGTTTCACAA GATGAAGCAG 
TTCCTCTCAG ACGAGCAGAA CATCCTGGCT CTTCGCAGCA TCCAAGGCAG ACAAAGAGAG 
AATCCAGGCC AGAGCCTGAA CAGACTCTTT CAGGAAGTAC CGAAACGAAG AAATGGGTCT 
GAAGGTAACA TCACCACCCG GATCCGAGCC TCCGAGACCG GGTCTGACGA AGCCATCAAG 
TCCATCTTGG AACAAGCCAA GAGGGAGCTG CAAGTCCAGA AAACCGCAGA GCCGGCCCAG 
CCTTCCTCGG CATCCAGTGG CGGGAGCGCA GACGACGCCA TCCGCTCCAT CCTGCAGCAG 
GCCCGCCGGG AGATGGAGGC CCAGCAGGCC GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG 
CCATCCCAGA CAGACATCGC CCTCCTGACC CCCAAGCTGA TACCCGCCTC GCCCATGTCG 
TCGGTGTCCA GTTACTCCCC TTTAGCCGTC TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC 
GCCAGCGCCC CTGCCCTGCC CAACCCGCCA GCCCTGAAGA AGGAGTCCCA GGATGCTCCC 
GGGCCGGACC TCCCAGGGGC GGCCGACCCC GCCGCACAGG GGGTCTTGAG ACACGTGAAG 
AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG GATCACTGGT GGAGCGCCGT CCAGCCCGAG 
AGGAAGAGCA GCGCTCCGCC CGAAGACCCC AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA 
AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC 
GAGTACTGGA AGGACTGGCC CAGCGCAGAG TCGCCCTACT CCCAGAGCTC AGAGCTGAGC 
CTGACCGGAG CCAGCCGCAG CGAGACGCCC CAGAACAGCC CACTGCCCTC CTCGCCCATT 
GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA GTCCCGCCAC TGACCCCCGA GCAGTACGAG 
ATCTACATGT ACCAGGAGGT GGACACCATC GAGCTCACCC GGCAGGTGAA GGAGAAGCTG 
GCCAAGAACG GCATCTGTCA GAGGATCTTC GGGGAGAAGG TGCTAGGCCT GTCACAGGGC 
AGTGTCAGTG ACATGCTGTC CCGGCCGAAG CCATGGAGCA AGCTGACCCA GAAAGGACGA 
GAACCCTTCA TACGGATGCA GCTCTGGCTG AACGGCGAGC TGGGCCAGGG CGTCCTGCCC 
GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG CACTCCGTGA CATCACTACA AGACCCCCTG 
CAGCAGGGCT GCGTGAGCTC AGAAAGCACT CCAAAGACCT CCGCCAGCTG CAGCCCTGCA 
CCCGAGTCAC CCATGAGCTC CAGTGAGTCA GTGAAGAGTC TGACTGAACT GGTCCAGCAG 
CCCTGCCCCC CCATCGAAAC CAGTAAGGAC AGCAAACCTC CAGAACCCAG CGACCCACCT 
GCCTCAGACT CCCAGCCCGC CACCCCACTG CCTCTCTCTG GACACTCAGC ACTCAGCATT 
CAAGAGCTGG TGGCCATGTC TCCAGAGCTG GACACCTACG GCATCACCAA GAGGGTCAAG 
GAGGTGTTGA CCGACAACAA CCTCGGTCAG CGCTTATTTG GGGAGACCAT CCTGGGGCTC 
ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT CGCCCCAAGC CCTGGCACAA GCTCAGTCTG 
AAGGGACGGG AGCCCTTTGT CCGGATGCAG CTGTGGCTGA ATGACCCCAA CAACGTGGAG 
AAGCTGATGG ACATGAAACG GATGGAAAAG AAAGCCTACA TGAAGCGGCG GCACAGCTCG 
GTCAGCGACA GCCAGCCCTG TGAGCCCCCG TCCGTTGGCA TCGACTACAG CCAGGGCGCC 
AGCCCCCAGC CCCAGCACCA GCTGAAGAAA CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA 
GAAGCTCTGA AACGAGCATA TCAGCAAAAG CCATACCCGT CACCAAAAAC CATCGAAGAA 
CTCGCCACCC AGCTCAACTT GAAAACCAGC ACCGTCATCA ACTGGTTCCA CAATTACAGG 
TCTCGGATCC GCAGAGAACT GTTCATTGAG GAGATTCAGG CCGGGAGCCA GGGCCAGGCC 
GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC GGCCGGGCCG CACCCAGCTC AGAAGGCGAC 
AGCTGCGACG GCGTGGAGGC CGCCGAGGGC CCAGGCGCCC CTGACCGGGC CGGGCCGAGT 
CTGGCCGAGC TGGGTCGAAC CGGGCCGGGC AGAGGGGCGG GCGCCGACTC CGCGGCCGGG 
ACCGTGTTGC GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059528.1
CDS	1..4098
Translation	MCYLQTRTSVYLKSVSQAPSSAFITFGPGSGITDLDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQTLQTALEKTRTELFDLKTKYDEEITAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEAELAAKEREIAQLVEDVQRLQANLSKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPAEGAGTQDASRPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPESRRPGPLPASPPSQLPRNTGEQASNTNGTHQFSPAGLTQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEIDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASSGGSADDAIRSILQQARREMEAQQAALEPALKPAPPSQTDIALLTPKLIPASPMSSVSSYSPLAVSLKKPPSAPDASAPALPNPPALKKESQDAPGPDLPGAADPAAQGVLRHVKSELGRGTVWKDHWWSAVQPERKSSAPPEDPKAEDASSSKEKGSGGQARAERSQLQGPSSSEYWKDWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPLSKPTKPSVPPLTPEQYEIYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPPIETSKDSKPPEPSDPPASDSQPATPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGIDYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEAAEGPGAPDRAGPSLAELGRTGPGRGAGADSAAGTVLRT

Target ORF information:

RefSeq Version	XM_006059466.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cut-like homeobox 1 (CUX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059466.1

ORF Insert Sequence:

ATGTGCTACC TTCAAACCAG GACCTCCGTG TATCTCAAAT CTGTGTCCCA AGCACCGTCC 
TCAGCCTTCA TCACCTTTGG GCCAGGTTCA GGCATCACCG ATCTGGATTT GCGCAAGCAG 
GTAGCACCGC TGCTGAAGAG CTTCCAAGGC GAGATTGATG CACTGAGTAA AAGAAGCAAA 
GAAGCTGAGG CAGCCTTCTT GAATGTCTAC AAGAGATTGA TTGATGTTCC AGATCCCGTT 
CCCGCGCTGG ACCTTGGGCA GCAGCTCCAG CTCAAAGTGC AGCGCCTGCA CGACATTGAG 
ACAGAGAACC AAAAACTTAG GGAAACGCTC GAAGAGTACA ACAAGGAATT CGCTGAAGTG 
AAAAATCAAG AGGTTACGAT AAAAGCACTT AAAGAGAAAA TCCGAGAATA TGAACAGACT 
CTGAAGAACC AGGCTGAGAC CATAGCTCTC GAAAAGGAAC AAAAGTTACA AAATGATTTT 
GCAGAAAAGG AGAGAAAGCT GCAGGAGACC CAGATGTCCA CCACCTCAAA GCTAGAAGAA 
GCCGAACATA AAGTCCAGAC TCTGCAAACA GCCCTGGAAA AAACTCGAAC AGAATTATTT 
GACCTGAAAA CCAAATATGA TGAAGAAATT ACTGCCAAGG CCGACGAAAT TGAAATGATC 
ATGACGGACC TTGAAAGAGC AAACCAGAGG GCAGAGGTGG CTCAAAGAGA GGCGGAGACC 
TTGAGGGAGC AGCTGTCGTC GGCCAACCAC TCTCTCCAGC TGGCCTCCCA GATCCAGAAG 
GCACCGGACG TGGAGCAGGC CATAGAGGTG CTGACCCGCT CCAGCCTGGA AGCTGAGTTG 
GCCGCCAAGG AGCGGGAGAT CGCCCAGCTG GTGGAGGACG TGCAGAGACT CCAGGCCAAC 
CTCAGCAAGC TGCGCGAGAA CTCAGCCAGC CAGATCTCCC AGCTCGAGCA GCAGCTGAGC 
GCCAAGAACA GCACCCTCAA GCAACTGGAA GAAAAACTCA AGGGACAGGC TGACTATGAA 
GAGGTGAAGA AAGAGCTAAA CATCCTGAAG TCCATGGAGT TTGCGCCGGC CGAGGGAGCT 
GGGACGCAGG ACGCATCCAG GCCCCTGGAG GTGCTGCTGC TGGAGAAGAA CCGCTCGCTG 
CAGTCCGAGA ACGCCGCGCT GCGCATCTCC AACAGCGACC TGAGCGGGTC AGCCAGGAGA 
AAAGGGAAAG ACCAGCCTGA GAGTCGGCGT CCTGGACCTT TGCCGGCCTC CCCTCCTTCT 
CAGTTGCCCC GCAACACGGG GGAGCAGGCT TCCAATACTA ATGGTACACA CCAGTTCTCA 
CCAGCGGGGT TAACGCAAGA CTTTTTCAGC TCATCCCTGG CAAGCCCCAG CCTGCCCCTG 
GCTTCTACGG GAAAATTTGC ACTAAACTCT CTCCTCCAAC GACAGCTAAT GCAGTCCTTC 
TACTCCAAGG CCATGCAGGA AGCAGGAAGC ACAAGCATGA TTTTTTCAAC AGGTCCATAC 
AGCACAAACT CCATATCTTC CCAAAGTCCA TTACAACAAA GCCCAGATGT AAATGGCATG 
GCCCCATCTC CCAGCCAGTC AGAAAGTGCT GGGAGCGTCT CCGAGGGCGA GGAGATAGAC 
ACTGCAGAAA TCGCCCGGCA GGTGAAAGAG CAGCTGATCA AGCACAATAT CGGACAGCGC 
ATTTTCGGAC ACTACGTCTT AGGACTGTCT CAGGGGTCCG TGAGCGAGAT CCTGGCCCGG 
CCCAAGCCGT GGAACAAACT GACGGTGCGC GGCAAGGAGC CGTTTCACAA GATGAAGCAG 
TTCCTCTCAG ACGAGCAGAA CATCCTGGCT CTTCGCAGCA TCCAAGGCAG ACAAAGAGAG 
AATCCAGGCC AGAGCCTGAA CAGACTCTTT CAGGAAGTAC CGAAACGAAG AAATGGGTCT 
GAAGGTAACA TCACCACCCG GATCCGAGCC TCCGAGACCG GGTCTGACGA AGCCATCAAG 
TCCATCTTGG AACAAGCCAA GAGGGAGCTG CAAGTCCAGA AAACCGCAGA GCCGGCCCAG 
CCTTCCTCGG CATCCAGTGG CGGGAGCGCA GACGACGCCA TCCGCTCCAT CCTGCAGCAG 
GCCCGCCGGG AGATGGAGGC CCAGCAGGCC GCCCTCGAGC CTGCCTTAAA GCCCGCGCCG 
CCATCCCAGA CAGACATCGC CCTCCTGACC CCCAAGCTGA TACCCGCCTC GCCCATGTCG 
TCGGTGTCCA GTTACTCCCC TTTAGCCGTC TCCCTGAAGA AGCCCCCCTC GGCTCCCGAC 
GCCAGCGCCC CTGCCCTGCC CAACCCGCCA GCCCTGAAGA AGGAGTCCCA GGATGCTCCC 
GGGCCGGACC TCCCAGGGGC GGCCGACCCC GCCGCACAGG GGGTCTTGAG ACACGTGAAG 
AGTGAGCTCG GCCGCGGCAC CGTGTGGAAG GATCACTGGT GGAGCGCCGT CCAGCCCGAG 
AGGAAGAGCA GCGCTCCGCC CGAAGACCCC AAGGCCGAAG ACGCCAGCAG CAGCAAAGAA 
AAAGGCAGCG GCGGGCAGGC GCGGGCGGAG CGCAGCCAGC TCCAGGGCCC CTCCTCCTCC 
GAGTACTGGA AGGACTGGCC CAGCGCAGAG TCGCCCTACT CCCAGAGCTC AGAGCTGAGC 
CTGACCGGAG CCAGCCGCAG CGAGACGCCC CAGAACAGCC CACTGCCCTC CTCGCCCATT 
GTGCCCCTGT CGAAGCCTAC CAAGCCCTCA GTCCCGCCAC TGACCCCCGA GCAGTACGAG 
ATCTACATGT ACCAGGAGGT GGACACCATC GAGCTCACCC GGCAGGTGAA GGAGAAGCTG 
GCCAAGAACG GCATCTGTCA GAGGATCTTC GGGGAGAAGG TGCTAGGCCT GTCACAGGGC 
AGTGTCAGTG ACATGCTGTC CCGGCCGAAG CCATGGAGCA AGCTGACCCA GAAAGGACGA 
GAACCCTTCA TACGGATGCA GCTCTGGCTG AACGGCGAGC TGGGCCAGGG CGTCCTGCCC 
GTCCAAGGGC AGCAGCAAGG GCCAGTCTTG CACTCCGTGA CATCACTACA AGACCCCCTG 
CAGCAGGGCT GCGTGAGCTC AGAAAGCACT CCAAAGACCT CCGCCAGCTG CAGCCCTGCA 
CCCGAGTCAC CCATGAGCTC CAGTGAGTCA GTGAAGAGTC TGACTGAACT GGTCCAGCAG 
CCCTGCCCCC CCATCGAAAC CAGTAAGGAC AGCAAACCTC CAGAACCCAG CGACCCACCT 
GCCTCAGACT CCCAGCCCGC CACCCCACTG CCTCTCTCTG GACACTCAGC ACTCAGCATT 
CAAGAGCTGG TGGCCATGTC TCCAGAGCTG GACACCTACG GCATCACCAA GAGGGTCAAG 
GAGGTGTTGA CCGACAACAA CCTCGGTCAG CGCTTATTTG GGGAGACCAT CCTGGGGCTC 
ACCCAAGGCT CTGTCTCTGA CCTCCTTGCT CGCCCCAAGC CCTGGCACAA GCTCAGTCTG 
AAGGGACGGG AGCCCTTTGT CCGGATGCAG CTGTGGCTGA ATGACCCCAA CAACGTGGAG 
AAGCTGATGG ACATGAAACG GATGGAAAAG AAAGCCTACA TGAAGCGGCG GCACAGCTCG 
GTCAGCGACA GCCAGCCCTG TGAGCCCCCG TCCGTTGGCA TCGACTACAG CCAGGGCGCC 
AGCCCCCAGC CCCAGCACCA GCTGAAGAAA CCCCGAGTAG TGCTGGCCCC CGAAGAGAAA 
GAAGCTCTGA AACGAGCATA TCAGCAAAAG CCATACCCGT CACCAAAAAC CATCGAAGAA 
CTCGCCACCC AGCTCAACTT GAAAACCAGC ACCGTCATCA ACTGGTTCCA CAATTACAGG 
TCTCGGATCC GCAGAGAACT GTTCATTGAG GAGATTCAGG CCGGGAGCCA GGGCCAGGCC 
GGGGCCAGCG ATTCGCCGTC CGCCCGGAGC GGCCGGGCCG CACCCAGCTC AGAAGGCGAC 
AGCTGCGACG GCGTGGAGGC CGCCGAGGGC CCAGGCGCCC CTGACCGGGC CGGGCCGAGT 
CTGGCCGAGC TGGGTCGAAC CGGGCCGGGC AGAGGGGCGG GCGCCGACTC CGCGGCCGGG 
ACCGTGTTGC GCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CUX1
Entrez Gene ID	102413697
Full Name	cut-like homeobox 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CUX1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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