ARAP2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following ARAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu69982 XM_025290199.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu69983 XM_025290200.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu29332 XM_006075678.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBu69972 XM_025290189.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu69973 XM_025290190.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu69974 XM_025290191.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu69975 XM_025290192.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu69976 XM_025290193.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu69977 XM_025290194.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu69978 XM_025290195.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu69979 XM_025290196.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu69980 XM_025290197.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu69981 XM_025290198.1
Latest version!
Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu69982
    Clone ID Related Accession (Same CDS sequence) XM_025290199.1
    Accession Version XM_025290199.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGCACAATTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145984.1
    CDS31..4383
    Translation

    Target ORF information:

    RefSeq Version XM_025290199.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290199.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGCACAATTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69983
    Clone ID Related Accession (Same CDS sequence) XM_025290200.1
    Accession Version XM_025290200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145985.1
    CDS31..4377
    Translation

    Target ORF information:

    RefSeq Version XM_025290200.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu29332
    Clone ID Related Accession (Same CDS sequence) XM_006075678.1
    Accession Version XM_006075678.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785651.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAACGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTCCTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTAAACTTG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAAGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006075740.1
    CDS413..5539
    Translation

    Target ORF information:

    RefSeq Version XM_006075678.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006075678.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAACGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTCCTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTAAACTTG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAAGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69972
    Clone ID Related Accession (Same CDS sequence) XM_025290189.1
    Accession Version XM_025290189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGCATGA AAATGATATA
    TGGCCACCAG CTGGAAAGGA ACGAAAACGT TCTATAACCA AAAATCCCAA AATTGGAGGT
    TTGCCTCTGA TTCCCATCCA GCATGACAGA AATGCAACCC AAGCCCGGAG AGAGACTGAG
    AGTGCAAAAG CAGAACTCGA AAGGCTGCGG CTCGGTGCAA AGCGTGACCA AGGTGGAGAT
    GGCACCTTAA AGGAGAAAGC CTCCATCGTG GCCCAGTGCC TGGAGCACAA GGACGAGAAA
    CTTCGAAATC GAACCAGAAA ACACCGGAGT TTCTACTGTC TGGAGGACAC AGAGGCTGAG
    GTCTTTCCAG GGCGACAGAA AGGCCATAAA GGCCTAAAGA CACTGAGGAA GACTGAGGAC
    AGGAATGGCA AAGATACTTT GGACTGCAAT AATAAGTTAC CACCAAAGGT CATCGAAGAG
    CTTAGCGTGG TTCTCCAGCG ATCAAGAACG CTTCCAAAAG AGTTGCAGGG TGAGCAGGTG
    GTGCAGAAGG AAATAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145974.1
    CDS144..5324
    Translation

    Target ORF information:

    RefSeq Version XM_025290189.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGCATGA AAATGATATA
    TGGCCACCAG CTGGAAAGGA ACGAAAACGT TCTATAACCA AAAATCCCAA AATTGGAGGT
    TTGCCTCTGA TTCCCATCCA GCATGACAGA AATGCAACCC AAGCCCGGAG AGAGACTGAG
    AGTGCAAAAG CAGAACTCGA AAGGCTGCGG CTCGGTGCAA AGCGTGACCA AGGTGGAGAT
    GGCACCTTAA AGGAGAAAGC CTCCATCGTG GCCCAGTGCC TGGAGCACAA GGACGAGAAA
    CTTCGAAATC GAACCAGAAA ACACCGGAGT TTCTACTGTC TGGAGGACAC AGAGGCTGAG
    GTCTTTCCAG GGCGACAGAA AGGCCATAAA GGCCTAAAGA CACTGAGGAA GACTGAGGAC
    AGGAATGGCA AAGATACTTT GGACTGCAAT AATAAGTTAC CACCAAAGGT CATCGAAGAG
    CTTAGCGTGG TTCTCCAGCG ATCAAGAACG CTTCCAAAAG AGTTGCAGGG TGAGCAGGTG
    GTGCAGAAGG AAATAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69973
    Clone ID Related Accession (Same CDS sequence) XM_025290190.1
    Accession Version XM_025290190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTACACATG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAGGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145975.1
    CDS1..5127
    Translation

    Target ORF information:

    RefSeq Version XM_025290190.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTACACATG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAGGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69974
    Clone ID Related Accession (Same CDS sequence) XM_025290191.1
    Accession Version XM_025290191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTTCCCAG
    GCTGGAGATT TGTTAATTGA AGTATATGTA GAAAGAAAGG AACCTGACTG CAGTATTATA
    ATTCGGATAT CTCCTGTGAT GGAAGCAGAA GAGTTAACTA ATGACATATT AGCAATAAAA
    AATATAATTC CAACAAAAGA TGATATCTGG GCCACATTTG AAGTCATAGA AAATGAAGAG
    CTCGAACGTC CTCTTCACTA CACAGAAAAT GTGTTGGAAC AGGTGCTTCG ATGGAGTTCA
    TTAGCTGAAC CTGGCTCTGC TTATCTTGTA GTGAAGAGAT TCTTAACCAT TGACACAATT
    AAACACTATA ATGAGAGGAA GGCCCTGGAA AATATCAAAG AGGGAGTCTT GAAAGTTAAA
    GAAGAACCAT CTAAAATACT CTCTGGAAAT AAGTTTCAAG ATCGGTATTG TGTTTTACGA
    GATGGGTACC TCCTTCTTTA CAAGGATCTG AAGAGTAGCA AACCTGACAA AATGTTTTCT
    CTCAGTTCAG TGAAGTTTTA TCTTGGAGTG AAAAAGAAGA TGAAGCCTCC AACAAGCTGG
    GGATTGACAG CATATTCTGA AAAACATCAC TGGCACCTGT GTTGTGAGAG TTCACAAACT
    CAAACGGAGT GGATGGCCAA TATCTTTATC GCCCAGCATG AAAATGATAT ATGGCCACCA
    GCTGGAAAGG AACGAAAACG TTCTATAACC AAAAATCCCA AAATTGGAGG TTTGCCTCTG
    ATTCCCATCC AGCATGACAG AAATGCAACC CAAGCCCGGA GAGAGACTGA GAGTGCAAAA
    GCAGAACTCG AAAGGCTGCG GCTCGGTGCA AAGCGTGACC AAGGTGGAGA TGGCACCTTA
    AAGGAGAAAG CCTCCATCGT GGCCCAGTGC CTGGAGCACA AGGACGAGAA ACTTCGAAAT
    CGAACCAGAA AACACCGGAG TTTCTACTGT CTGGAGGACA CAGAGGCTGA GGTCTTTCCA
    GGGCGACAGA AAGGCCATAA AGGCCTAAAG ACACTGAGGA AGACTGAGGA CAGGAATGGC
    AAAGATACTT TGGACTGCAA TAATAAGTTA CCACCAAAGG TCATCGAAGA GCTTAGCGTG
    GTTCTCCAGC GATCAAGAAC GCTTCCAAAA GAGTTGCAGG GTGAGCAGGT GGTGCAGAAG
    GAAATAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145976.1
    CDS144..5255
    Translation

    Target ORF information:

    RefSeq Version XM_025290191.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTTCCCAG
    GCTGGAGATT TGTTAATTGA AGTATATGTA GAAAGAAAGG AACCTGACTG CAGTATTATA
    ATTCGGATAT CTCCTGTGAT GGAAGCAGAA GAGTTAACTA ATGACATATT AGCAATAAAA
    AATATAATTC CAACAAAAGA TGATATCTGG GCCACATTTG AAGTCATAGA AAATGAAGAG
    CTCGAACGTC CTCTTCACTA CACAGAAAAT GTGTTGGAAC AGGTGCTTCG ATGGAGTTCA
    TTAGCTGAAC CTGGCTCTGC TTATCTTGTA GTGAAGAGAT TCTTAACCAT TGACACAATT
    AAACACTATA ATGAGAGGAA GGCCCTGGAA AATATCAAAG AGGGAGTCTT GAAAGTTAAA
    GAAGAACCAT CTAAAATACT CTCTGGAAAT AAGTTTCAAG ATCGGTATTG TGTTTTACGA
    GATGGGTACC TCCTTCTTTA CAAGGATCTG AAGAGTAGCA AACCTGACAA AATGTTTTCT
    CTCAGTTCAG TGAAGTTTTA TCTTGGAGTG AAAAAGAAGA TGAAGCCTCC AACAAGCTGG
    GGATTGACAG CATATTCTGA AAAACATCAC TGGCACCTGT GTTGTGAGAG TTCACAAACT
    CAAACGGAGT GGATGGCCAA TATCTTTATC GCCCAGCATG AAAATGATAT ATGGCCACCA
    GCTGGAAAGG AACGAAAACG TTCTATAACC AAAAATCCCA AAATTGGAGG TTTGCCTCTG
    ATTCCCATCC AGCATGACAG AAATGCAACC CAAGCCCGGA GAGAGACTGA GAGTGCAAAA
    GCAGAACTCG AAAGGCTGCG GCTCGGTGCA AAGCGTGACC AAGGTGGAGA TGGCACCTTA
    AAGGAGAAAG CCTCCATCGT GGCCCAGTGC CTGGAGCACA AGGACGAGAA ACTTCGAAAT
    CGAACCAGAA AACACCGGAG TTTCTACTGT CTGGAGGACA CAGAGGCTGA GGTCTTTCCA
    GGGCGACAGA AAGGCCATAA AGGCCTAAAG ACACTGAGGA AGACTGAGGA CAGGAATGGC
    AAAGATACTT TGGACTGCAA TAATAAGTTA CCACCAAAGG TCATCGAAGA GCTTAGCGTG
    GTTCTCCAGC GATCAAGAAC GCTTCCAAAA GAGTTGCAGG GTGAGCAGGT GGTGCAGAAG
    GAAATAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69975
    Clone ID Related Accession (Same CDS sequence) XM_025290192.1
    Accession Version XM_025290192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGAGTGC AAAAGCAGAA
    CTCGAAAGGC TGCGGCTCGG TGCAAAGCGT GACCAAGGTG GAGATGGCAC CTTAAAGGAG
    AAAGCCTCCA TCGTGGCCCA GTGCCTGGAG CACAAGGACG AGAAACTTCG AAATCGAACC
    AGAAAACACC GGAGTTTCTA CTGTCTGGAG GACACAGAGG CTGAGGTCTT TCCAGGGCGA
    CAGAAAGGCC ATAAAGGCCT AAAGACACTG AGGAAGACTG AGGACAGGAA TGGCAAAGAT
    ACTTTGGACT GCAATAATAA GTTACCACCA AAGGTCATCG AAGAGCTTAG CGTGGTTCTC
    CAGCGATCAA GAACGCTTCC AAAAGAGTTG CAGGGTGAGC AGGTGGTGCA GAAGGAAATA
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145977.1
    CDS31..5076
    Translation

    Target ORF information:

    RefSeq Version XM_025290192.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGAGTGC AAAAGCAGAA
    CTCGAAAGGC TGCGGCTCGG TGCAAAGCGT GACCAAGGTG GAGATGGCAC CTTAAAGGAG
    AAAGCCTCCA TCGTGGCCCA GTGCCTGGAG CACAAGGACG AGAAACTTCG AAATCGAACC
    AGAAAACACC GGAGTTTCTA CTGTCTGGAG GACACAGAGG CTGAGGTCTT TCCAGGGCGA
    CAGAAAGGCC ATAAAGGCCT AAAGACACTG AGGAAGACTG AGGACAGGAA TGGCAAAGAT
    ACTTTGGACT GCAATAATAA GTTACCACCA AAGGTCATCG AAGAGCTTAG CGTGGTTCTC
    CAGCGATCAA GAACGCTTCC AAAAGAGTTG CAGGGTGAGC AGGTGGTGCA GAAGGAAATA
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69976
    Clone ID Related Accession (Same CDS sequence) XM_025290193.1
    Accession Version XM_025290193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGAAAA
    CGCATGTTTC AGAAGAGATG GGTGAAATTT GATGGCCTTA GCATTTCTTA TTACAATAAT
    GATAAGGAAG AGAAGCTAAA ACCCCCAAAA TGGAAAATAA GGAGAAGAAA AAGTTATTCT
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTACACATG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAGGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145978.1
    CDS31..4857
    Translation

    Target ORF information:

    RefSeq Version XM_025290193.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGAAAA
    CGCATGTTTC AGAAGAGATG GGTGAAATTT GATGGCCTTA GCATTTCTTA TTACAATAAT
    GATAAGGAAG AGAAGCTAAA ACCCCCAAAA TGGAAAATAA GGAGAAGAAA AAGTTATTCT
    GAGAAGTATT CAAAAGGAAT AATCCCCCTT TCTGCTATAT CTACGGTACG AGTTCAAGGA
    GACAACAAAT TTGAAGTTGT TACAACACAA AGAACTTTTG TTTTTAGAGT AGAAAAAGAA
    GAGGAAAGAA ATGACTGGAT CAGCCTACTA TTAAATGCAC TGAAGTTACA GGCCCTTTCT
    TCGCAGTCTC AAGCTGCTAT TGCACCTGAG AAATGTGGAT ATCTTGAACT GAGAGGCTAT
    AAAGCTAAAA TTTTTACTGT GTTAAGTGGA AACAGTGTGT GGCTTTGCAA AAATGAACAG
    GATTTTAAGA GTGGACTTGG TATTACCATA ATCCCTATGA ATGTAGCAAA TGTAAAACAA
    GTGGACCGAA CTGTAAAACA GTCTTTTGAA ATAATCACTC CTTACAGGAG TTTTAGCTTT
    ACAGCTGAGT CTGAAAAAGA GAAACAAGAG TGGATTGAAG CTGTGCAGCA ATCAATAGCA
    GAAACTCTCT CTGATTATGA AGTAGCTGAG AAGATTTGGT ACAATGAGTC CAACAGGAGC
    TGTGCAGATT GTAAAGCCCC AGATCCTGAC TGGGCCTCCA TCAATCTCTG TGTTGTCATC
    TGCAAGAAGT GTGCAGGGCA ACATAGATCT TTAGGACCAA AAGATTCCAA GGTTAGAAGT
    CTGAAAATGG ATGCCAGCAT TTGGAGCAAT GAGCTCATTG AGCTTTTCAT TGTCATTGGA
    AACAAAAGAG CTAATGACTT TTGGGCTGGT AATCTTCAAA AAGATGAAGA ATTACACATG
    GACTGTTCAG TAGAAAAGAG AAAAAATTTT ATCACTCAGA AGTACAAAGA AGGAAGATTC
    AGAAAAACCC TTTTGGCGTC TCTCACCAAG GAAGAATTAA ATAAGGCTCT GTGTGCTGCT
    GTGGTGAAAC CAGATGTTCT GGAAACAATG GCTTTGCTGT TCAGTGGAGC AGATGTCATG
    TGTGCAACAG GTGATCCTGA GCACAGCACC CCCTATCTGC TGGCCAAGAA GGCAGGGCAG
    AGTCTGCAGA TGGAATTTCT CTACCATAAC AAGTTCTCAG ATTTCCCTCA ACATGACATA
    TATTCTGAAG GTCGATTACA TCAAGAGTCC TCCCAGTCTA CGTTCCTCTG TGACTTTTTG
    TATCAGGCTC CTGCTGGTGC TTCTAAACTC TCTTCAGAAA AAAAACTGCT TGAAGAGACA
    AATAAAAAGT GGTGTGTTCT AGAAGGAGGC TTCTTGAGTT ACTATGAAAA TGATAAGTCT
    ACCACGCCTA ATGGCACCAT TAATATCAAT GAAGTCATCT GCCTGGCTGT GCACAAAGAA
    GACTTCTGTT TAAATACTGG GCCCATCTTT ACTTTTGAGA TTTATTTACC CTCAGAACGT
    GCATTTTTAT TTGGAGCTGA AACATCTCAA GTGCAAAGAA AATGGACAGA AGCAATAGCC
    AAGCATTTTG TTCCCTTTGT TGCTGAAAAC TTAACAGAAG CTGACTATGA TTTGATTGGT
    CAACTCTACT ACAAAGACTG CCACGCCCTG GATCAGTGGA GAAAAGGCTG GTTTGCAATG
    GATAAATCTA ATTTGCGTTT TTGCCTTCAA ATGCAAGAAG GTCAGGAGGA TAGAATGCAC
    TTAAGACGAC TACAAGAACT AACAATTAGC ACAGTCATTC AAAATGGGGA AAAATTGGAT
    GTTTTGCTCT TGGTAGAAAA AGGGAGAACA TTATACATCC ATGGGCATAC CAAGTTGGAT
    TTCACAGTCT GGCATACTGC AATTGAAAAA GCAGCGGGTA CAGATGGGAA TGCATTACAA
    GACCAGCAGC TCGGCAGAAA TGACGTTCCC ATTATCGTGA ACAGCTGTAT AGCATTTGTT
    ACACAGTATG GTTTAGGATA CAAAAATATC TATCAAAAGA ATGGTGATCC ATTACATGTA
    AGTGAACTCC TGGAGAGTTT CAAAAAGGAT GCAAGAAGCT TTAAATTGAG GGCTGGAAAA
    CATCAACTTG AAGATGTGAC AGGTGTATTG AAGAGTTTTC TCTCCGACAT TGATGATGCG
    CTTCTCACTA AGGAGCTCTA CCCGTATTGG ATCTCTGCCT TAGATACCCA AGATGACAAG
    GAAAGAATTA AGAAGTATGG AGCATTTATA CGTACTCTTC CTGGGGTGAA CCGAGCAACA
    TTGGCAGCTA TAATTGAACA CCTTTACAGG GTTCAGAAAT GCTCAGAAAT CAATCACATG
    AGTGCCCACA ATTTGGCCTT GGTTTTTTCC TCCTGTTTCT TTCAAACTAA GGGACAAACT
    AGTGAAGAAG TGAATGTAAT CGAGGACTTA ATTAATAATT ATGTTGAAAT TTTTGAGGTT
    AAAGAAGACC AAGTCAAACA AATGGACATA GAGAATAGCT TTATTACCAA GTGGAAAGAC
    ACTCAAGTTT CCCAGGCTGG AGATTTGTTA ATTGAAGTAT ATGTAGAAAG AAAGGAACCT
    GACTGCAGTA TTATAATTCG GATATCTCCT GTGATGGAAG CAGAAGAGTT AACTAATGAC
    ATATTAGCAA TAAAAAATAT AATTCCAACA AAAGATGATA TCTGGGCCAC ATTTGAAGTC
    ATAGAAAATG AAGAGCTCGA ACGTCCTCTT CACTACACAG AAAATGTGTT GGAACAGGTG
    CTTCGATGGA GTTCATTAGC TGAACCTGGC TCTGCTTATC TTGTAGTGAA GAGATTCTTA
    ACCATTGACA CAATTAAACA CTATAATGAG AGGAAGGCCC TGGAAAATAT CAAAGAGGGA
    GTCTTGAAAG TTAAAGAAGA ACCATCTAAA ATACTCTCTG GAAATAAGTT TCAAGATCGG
    TATTGTGTTT TACGAGATGG GTACCTCCTT CTTTACAAGG ATCTGAAGAG TAGCAAACCT
    GACAAAATGT TTTCTCTCAG TTCAGTGAAG TTTTATCTTG GAGTGAAAAA GAAGATGAAG
    CCTCCAACAA GCTGGGGATT GACAGCATAT TCTGAAAAAC ATCACTGGCA CCTGTGTTGT
    GAGAGTTCAC AAACTCAAAC GGAGTGGATG GCCAATATCT TTATCGCCCA GCATGAAAAT
    GATATATGGC CACCAGCTGG AAAGGAACGA AAACGTTCTA TAACCAAAAA TCCCAAAATT
    GGAGGTTTGC CTCTGATTCC CATCCAGCAT GACAGAAATG CAACCCAAGC CCGGAGAGAG
    ACTGAGAGTG CAAAAGCAGA ACTCGAAAGG CTGCGGCTCG GTGCAAAGCG TGACCAAGGT
    GGAGATGGCA CCTTAAAGGA GAAAGCCTCC ATCGTGGCCC AGTGCCTGGA GCACAAGGAC
    GAGAAACTTC GAAATCGAAC CAGAAAACAC CGGAGTTTCT ACTGTCTGGA GGACACAGAG
    GCTGAGGTCT TTCCAGGGCG ACAGAAAGGC CATAAAGGCC TAAAGACACT GAGGAAGACT
    GAGGACAGGA ATGGCAAAGA TACTTTGGAC TGCAATAATA AGTTACCACC AAAGGTCATC
    GAAGAGCTTA GCGTGGTTCT CCAGCGATCA AGAACGCTTC CAAAAGAGTT GCAGGGTGAG
    CAGGTGGTGC AGAAGGAAAT AAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69977
    Clone ID Related Accession (Same CDS sequence) XM_025290194.1
    Accession Version XM_025290194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGAAAA
    CGCATGTTTC AGAAGAGATG GGTGAAATTT GATGGCCTTA GCATTTCTTA TTACAATAAT
    GATAAGGAGA AGTATTCAAA AGGAATAATC CCCCTTTCTG CTATATCTAC GGTACGAGTT
    CAAGGAGACA ACAAATTTGA AGTTGTTACA ACACAAAGAA CTTTTGTTTT TAGAGTAGAA
    AAAGAAGAGG AAAGAAATGA CTGGATCAGC CTACTATTAA ATGCACTGAA GTTACAGGCC
    CTTTCTTCGC AGTCTCAAGC TGCTATTGCA CCTGAGAAAT GTGGATATCT TGAACTGAGA
    GGCTATAAAG CTAAAATTTT TACTGTGTTA AGTGGAAACA GTGTGTGGCT TTGCAAAAAT
    GAACAGGATT TTAAGAGTGG ACTTGGTATT ACCATAATCC CTATGAATGT AGCAAATGTA
    AAACAAGTGG ACCGAACTGT AAAACAGTCT TTTGAAATAA TCACTCCTTA CAGGAGTTTT
    AGCTTTACAG CTGAGTCTGA AAAAGAGAAA CAAGAGTGGA TTGAAGCTGT GCAGCAATCA
    ATAGCAGAAA CTCTCTCTGA TTATGAAGTA GCTGAGAAGA TTTGGTACAA TGAGTCCAAC
    AGGAGCTGTG CAGATTGTAA AGCCCCAGAT CCTGACTGGG CCTCCATCAA TCTCTGTGTT
    GTCATCTGCA AGAAGTGTGC AGGGCAACAT AGATCTTTAG GACCAAAAGA TTCCAAGGTT
    AGAAGTCTGA AAATGGATGC CAGCATTTGG AGCAATGAGC TCATTGAGCT TTTCATTGTC
    ATTGGAAACA AAAGAGCTAA TGACTTTTGG GCTGGTAATC TTCAAAAAGA TGAAGAATTA
    CACATGGACT GTTCAGTAGA AAAGAGAAAA AATTTTATCA CTCAGAAGTA CAAAGAAGGA
    AGATTCAGAA AAACCCTTTT GGCGTCTCTC ACCAAGGAAG AATTAAATAA GGCTCTGTGT
    GCTGCTGTGG TGAAACCAGA TGTTCTGGAA ACAATGGCTT TGCTGTTCAG TGGAGCAGAT
    GTCATGTGTG CAACAGGTGA TCCTGAGCAC AGCACCCCCT ATCTGCTGGC CAAGAAGGCA
    GGGCAGAGTC TGCAGATGGA ATTTCTCTAC CATAACAAGT TCTCAGATTT CCCTCAACAT
    GACATATATT CTGAAGGTCG ATTACATCAA GAGTCCTCCC AGTCTACGTT CCTCTGTGAC
    TTTTTGTATC AGGCTCCTGC TGGTGCTTCT AAACTCTCTT CAGAAAAAAA ACTGCTTGAA
    GAGACAAATA AAAAGTGGTG TGTTCTAGAA GGAGGCTTCT TGAGTTACTA TGAAAATGAT
    AAGTCTACCA CGCCTAATGG CACCATTAAT ATCAATGAAG TCATCTGCCT GGCTGTGCAC
    AAAGAAGACT TCTGTTTAAA TACTGGGCCC ATCTTTACTT TTGAGATTTA TTTACCCTCA
    GAACGTGCAT TTTTATTTGG AGCTGAAACA TCTCAAGTGC AAAGAAAATG GACAGAAGCA
    ATAGCCAAGC ATTTTGTTCC CTTTGTTGCT GAAAACTTAA CAGAAGCTGA CTATGATTTG
    ATTGGTCAAC TCTACTACAA AGACTGCCAC GCCCTGGATC AGTGGAGAAA AGGCTGGTTT
    GCAATGGATA AATCTAATTT GCGTTTTTGC CTTCAAATGC AAGAAGGTCA GGAGGATAGA
    ATGCACTTAA GACGACTACA AGAACTAACA ATTAGCACAG TCATTCAAAA TGGGGAAAAA
    TTGGATGTTT TGCTCTTGGT AGAAAAAGGG AGAACATTAT ACATCCATGG GCATACCAAG
    TTGGATTTCA CAGTCTGGCA TACTGCAATT GAAAAAGCAG CGGGTACAGA TGGGAATGCA
    TTACAAGACC AGCAGCTCGG CAGAAATGAC GTTCCCATTA TCGTGAACAG CTGTATAGCA
    TTTGTTACAC AGTATGGTTT AGGATACAAA AATATCTATC AAAAGAATGG TGATCCATTA
    CATGTAAGTG AACTCCTGGA GAGTTTCAAA AAGGATGCAA GAAGCTTTAA ATTGAGGGCT
    GGAAAACATC AACTTGAAGA TGTGACAGGT GTATTGAAGA GTTTTCTCTC CGACATTGAT
    GATGCGCTTC TCACTAAGGA GCTCTACCCG TATTGGATCT CTGCCTTAGA TACCCAAGAT
    GACAAGGAAA GAATTAAGAA GTATGGAGCA TTTATACGTA CTCTTCCTGG GGTGAACCGA
    GCAACATTGG CAGCTATAAT TGAACACCTT TACAGGGTTC AGAAATGCTC AGAAATCAAT
    CACATGAGTG CCCACAATTT GGCCTTGGTT TTTTCCTCCT GTTTCTTTCA AACTAAGGGA
    CAAACTAGTG AAGAAGTGAA TGTAATCGAG GACTTAATTA ATAATTATGT TGAAATTTTT
    GAGGTTAAAG AAGACCAAGT CAAACAAATG GACATAGAGA ATAGCTTTAT TACCAAGTGG
    AAAGACACTC AAGTTTCCCA GGCTGGAGAT TTGTTAATTG AAGTATATGT AGAAAGAAAG
    GAACCTGACT GCAGTATTAT AATTCGGATA TCTCCTGTGA TGGAAGCAGA AGAGTTAACT
    AATGACATAT TAGCAATAAA AAATATAATT CCAACAAAAG ATGATATCTG GGCCACATTT
    GAAGTCATAG AAAATGAAGA GCTCGAACGT CCTCTTCACT ACACAGAAAA TGTGTTGGAA
    CAGGTGCTTC GATGGAGTTC ATTAGCTGAA CCTGGCTCTG CTTATCTTGT AGTGAAGAGA
    TTCTTAACCA TTGACACAAT TAAACACTAT AATGAGAGGA AGGCCCTGGA AAATATCAAA
    GAGGGAGTCT TGAAAGTTAA AGAAGAACCA TCTAAAATAC TCTCTGGAAA TAAGTTTCAA
    GATCGGTATT GTGTTTTACG AGATGGGTAC CTCCTTCTTT ACAAGGATCT GAAGAGTAGC
    AAACCTGACA AAATGTTTTC TCTCAGTTCA GTGAAGTTTT ATCTTGGAGT GAAAAAGAAG
    ATGAAGCCTC CAACAAGCTG GGGATTGACA GCATATTCTG AAAAACATCA CTGGCACCTG
    TGTTGTGAGA GTTCACAAAC TCAAACGGAG TGGATGGCCA ATATCTTTAT CGCCCAGCAT
    GAAAATGATA TATGGCCACC AGCTGGAAAG GAACGAAAAC GTTCTATAAC CAAAAATCCC
    AAAATTGGAG GTTTGCCTCT GATTCCCATC CAGCATGACA GAAATGCAAC CCAAGCCCGG
    AGAGAGACTG AGAGTGCAAA AGCAGAACTC GAAAGGCTGC GGCTCGGTGC AAAGCGTGAC
    CAAGGTGGAG ATGGCACCTT AAAGGAGAAA GCCTCCATCG TGGCCCAGTG CCTGGAGCAC
    AAGGACGAGA AACTTCGAAA TCGAACCAGA AAACACCGGA GTTTCTACTG TCTGGAGGAC
    ACAGAGGCTG AGGTCTTTCC AGGGCGACAG AAAGGCCATA AAGGCCTAAA GACACTGAGG
    AAGACTGAGG ACAGGAATGG CAAAGATACT TTGGACTGCA ATAATAAGTT ACCACCAAAG
    GTCATCGAAG AGCTTAGCGT GGTTCTCCAG CGATCAAGAA CGCTTCCAAA AGAGTTGCAG
    GGTGAGCAGG TGGTGCAGAA GGAAATAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145979.1
    CDS144..4916
    Translation

    Target ORF information:

    RefSeq Version XM_025290194.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGAAAA
    CGCATGTTTC AGAAGAGATG GGTGAAATTT GATGGCCTTA GCATTTCTTA TTACAATAAT
    GATAAGGAGA AGTATTCAAA AGGAATAATC CCCCTTTCTG CTATATCTAC GGTACGAGTT
    CAAGGAGACA ACAAATTTGA AGTTGTTACA ACACAAAGAA CTTTTGTTTT TAGAGTAGAA
    AAAGAAGAGG AAAGAAATGA CTGGATCAGC CTACTATTAA ATGCACTGAA GTTACAGGCC
    CTTTCTTCGC AGTCTCAAGC TGCTATTGCA CCTGAGAAAT GTGGATATCT TGAACTGAGA
    GGCTATAAAG CTAAAATTTT TACTGTGTTA AGTGGAAACA GTGTGTGGCT TTGCAAAAAT
    GAACAGGATT TTAAGAGTGG ACTTGGTATT ACCATAATCC CTATGAATGT AGCAAATGTA
    AAACAAGTGG ACCGAACTGT AAAACAGTCT TTTGAAATAA TCACTCCTTA CAGGAGTTTT
    AGCTTTACAG CTGAGTCTGA AAAAGAGAAA CAAGAGTGGA TTGAAGCTGT GCAGCAATCA
    ATAGCAGAAA CTCTCTCTGA TTATGAAGTA GCTGAGAAGA TTTGGTACAA TGAGTCCAAC
    AGGAGCTGTG CAGATTGTAA AGCCCCAGAT CCTGACTGGG CCTCCATCAA TCTCTGTGTT
    GTCATCTGCA AGAAGTGTGC AGGGCAACAT AGATCTTTAG GACCAAAAGA TTCCAAGGTT
    AGAAGTCTGA AAATGGATGC CAGCATTTGG AGCAATGAGC TCATTGAGCT TTTCATTGTC
    ATTGGAAACA AAAGAGCTAA TGACTTTTGG GCTGGTAATC TTCAAAAAGA TGAAGAATTA
    CACATGGACT GTTCAGTAGA AAAGAGAAAA AATTTTATCA CTCAGAAGTA CAAAGAAGGA
    AGATTCAGAA AAACCCTTTT GGCGTCTCTC ACCAAGGAAG AATTAAATAA GGCTCTGTGT
    GCTGCTGTGG TGAAACCAGA TGTTCTGGAA ACAATGGCTT TGCTGTTCAG TGGAGCAGAT
    GTCATGTGTG CAACAGGTGA TCCTGAGCAC AGCACCCCCT ATCTGCTGGC CAAGAAGGCA
    GGGCAGAGTC TGCAGATGGA ATTTCTCTAC CATAACAAGT TCTCAGATTT CCCTCAACAT
    GACATATATT CTGAAGGTCG ATTACATCAA GAGTCCTCCC AGTCTACGTT CCTCTGTGAC
    TTTTTGTATC AGGCTCCTGC TGGTGCTTCT AAACTCTCTT CAGAAAAAAA ACTGCTTGAA
    GAGACAAATA AAAAGTGGTG TGTTCTAGAA GGAGGCTTCT TGAGTTACTA TGAAAATGAT
    AAGTCTACCA CGCCTAATGG CACCATTAAT ATCAATGAAG TCATCTGCCT GGCTGTGCAC
    AAAGAAGACT TCTGTTTAAA TACTGGGCCC ATCTTTACTT TTGAGATTTA TTTACCCTCA
    GAACGTGCAT TTTTATTTGG AGCTGAAACA TCTCAAGTGC AAAGAAAATG GACAGAAGCA
    ATAGCCAAGC ATTTTGTTCC CTTTGTTGCT GAAAACTTAA CAGAAGCTGA CTATGATTTG
    ATTGGTCAAC TCTACTACAA AGACTGCCAC GCCCTGGATC AGTGGAGAAA AGGCTGGTTT
    GCAATGGATA AATCTAATTT GCGTTTTTGC CTTCAAATGC AAGAAGGTCA GGAGGATAGA
    ATGCACTTAA GACGACTACA AGAACTAACA ATTAGCACAG TCATTCAAAA TGGGGAAAAA
    TTGGATGTTT TGCTCTTGGT AGAAAAAGGG AGAACATTAT ACATCCATGG GCATACCAAG
    TTGGATTTCA CAGTCTGGCA TACTGCAATT GAAAAAGCAG CGGGTACAGA TGGGAATGCA
    TTACAAGACC AGCAGCTCGG CAGAAATGAC GTTCCCATTA TCGTGAACAG CTGTATAGCA
    TTTGTTACAC AGTATGGTTT AGGATACAAA AATATCTATC AAAAGAATGG TGATCCATTA
    CATGTAAGTG AACTCCTGGA GAGTTTCAAA AAGGATGCAA GAAGCTTTAA ATTGAGGGCT
    GGAAAACATC AACTTGAAGA TGTGACAGGT GTATTGAAGA GTTTTCTCTC CGACATTGAT
    GATGCGCTTC TCACTAAGGA GCTCTACCCG TATTGGATCT CTGCCTTAGA TACCCAAGAT
    GACAAGGAAA GAATTAAGAA GTATGGAGCA TTTATACGTA CTCTTCCTGG GGTGAACCGA
    GCAACATTGG CAGCTATAAT TGAACACCTT TACAGGGTTC AGAAATGCTC AGAAATCAAT
    CACATGAGTG CCCACAATTT GGCCTTGGTT TTTTCCTCCT GTTTCTTTCA AACTAAGGGA
    CAAACTAGTG AAGAAGTGAA TGTAATCGAG GACTTAATTA ATAATTATGT TGAAATTTTT
    GAGGTTAAAG AAGACCAAGT CAAACAAATG GACATAGAGA ATAGCTTTAT TACCAAGTGG
    AAAGACACTC AAGTTTCCCA GGCTGGAGAT TTGTTAATTG AAGTATATGT AGAAAGAAAG
    GAACCTGACT GCAGTATTAT AATTCGGATA TCTCCTGTGA TGGAAGCAGA AGAGTTAACT
    AATGACATAT TAGCAATAAA AAATATAATT CCAACAAAAG ATGATATCTG GGCCACATTT
    GAAGTCATAG AAAATGAAGA GCTCGAACGT CCTCTTCACT ACACAGAAAA TGTGTTGGAA
    CAGGTGCTTC GATGGAGTTC ATTAGCTGAA CCTGGCTCTG CTTATCTTGT AGTGAAGAGA
    TTCTTAACCA TTGACACAAT TAAACACTAT AATGAGAGGA AGGCCCTGGA AAATATCAAA
    GAGGGAGTCT TGAAAGTTAA AGAAGAACCA TCTAAAATAC TCTCTGGAAA TAAGTTTCAA
    GATCGGTATT GTGTTTTACG AGATGGGTAC CTCCTTCTTT ACAAGGATCT GAAGAGTAGC
    AAACCTGACA AAATGTTTTC TCTCAGTTCA GTGAAGTTTT ATCTTGGAGT GAAAAAGAAG
    ATGAAGCCTC CAACAAGCTG GGGATTGACA GCATATTCTG AAAAACATCA CTGGCACCTG
    TGTTGTGAGA GTTCACAAAC TCAAACGGAG TGGATGGCCA ATATCTTTAT CGCCCAGCAT
    GAAAATGATA TATGGCCACC AGCTGGAAAG GAACGAAAAC GTTCTATAAC CAAAAATCCC
    AAAATTGGAG GTTTGCCTCT GATTCCCATC CAGCATGACA GAAATGCAAC CCAAGCCCGG
    AGAGAGACTG AGAGTGCAAA AGCAGAACTC GAAAGGCTGC GGCTCGGTGC AAAGCGTGAC
    CAAGGTGGAG ATGGCACCTT AAAGGAGAAA GCCTCCATCG TGGCCCAGTG CCTGGAGCAC
    AAGGACGAGA AACTTCGAAA TCGAACCAGA AAACACCGGA GTTTCTACTG TCTGGAGGAC
    ACAGAGGCTG AGGTCTTTCC AGGGCGACAG AAAGGCCATA AAGGCCTAAA GACACTGAGG
    AAGACTGAGG ACAGGAATGG CAAAGATACT TTGGACTGCA ATAATAAGTT ACCACCAAAG
    GTCATCGAAG AGCTTAGCGT GGTTCTCCAG CGATCAAGAA CGCTTCCAAA AGAGTTGCAG
    GGTGAGCAGG TGGTGCAGAA GGAAATAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69978
    Clone ID Related Accession (Same CDS sequence) XM_025290195.1
    Accession Version XM_025290195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGTACAG AAGGCATATT
    TCTGAAGCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145980.1
    CDS31..4722
    Translation

    Target ORF information:

    RefSeq Version XM_025290195.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGTACAG AAGGCATATT
    TCTGAAGCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69979
    Clone ID Related Accession (Same CDS sequence) XM_025290196.1
    Accession Version XM_025290196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGCAGAT CACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145981.1
    CDS31..4707
    Translation

    Target ORF information:

    RefSeq Version XM_025290196.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGCAGAT CACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69980
    Clone ID Related Accession (Same CDS sequence) XM_025290197.1
    Accession Version XM_025290197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGATCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145982.1
    CDS31..4704
    Translation

    Target ORF information:

    RefSeq Version XM_025290197.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGAGTAGCAA ACCTGACAAA
    ATGTTTTCTC TCAGTTCAGT GAAGTTTTAT CTTGGAGTGA AAAAGAAGAT GAAGCCTCCA
    ACAAGCTGGG GATTGACAGC ATATTCTGAA AAACATCACT GGCACCTGTG TTGTGAGAGT
    TCACAAACTC AAACGGAGTG GATGGCCAAT ATCTTTATCG CCCAGATCAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu69981
    Clone ID Related Accession (Same CDS sequence) XM_025290198.1
    Accession Version XM_025290198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGCAAGGAAG CCTTCAGGTG
    GACATAAATA AATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145983.1
    CDS31..4548
    Translation

    Target ORF information:

    RefSeq Version XM_025290198.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCTCAG TCAGCGAAGT GAATGTTGAT ATCAAAGATT TCCTAATGAG CATTAATCTG 
    GAGCAGTACC TCTTAAACTT CCGAGAATTT GGTTTTAATA ATGTGAGGGA CTGTGCAGAA
    ATAAATGACA GTGTGCTCCT CAAAGTTGGA ATATCACCTA CAGGACACCG GAGGAGGATA
    CTTAAACAGT TACAGATAAT CCTGTCAAAA ATGCAAGATA TCCCAATATA TGCAAATATT
    CACAAAACAA AGAAAAATGA TGAGACTTCG AAGGATCACC AGGCTCGGTC TTTTGCTCAG
    AATACCTCCT TAGAATTTTC AGATTCACAC AGTGTTCAGA TACCTAGCCG GATACAGTTG
    GAGACTGTTA CAAAAAATGT TGGAGAAAAT GAGGTTTGTG TGGAAAATAG CCAGTCTCTT
    AAATCAGATG ATAAGCTGAC TCTTCCTACA CACAACTTCC CCATTCCAGA AGATGAACCA
    CACTCGAATT CAGGTTCTTT TCAAGATTCT TTACTTCGTA ATGAAATTAC CGACATAGAA
    TCTTTGATAA CAGAGAAGAC TGTGGGCTGC ACAGCTGAAG AAGAACACAT AGGAAGAGAT
    GATTTGATTT CAGAAAATGT AGGCAAGCTT CCTAATGCAG ACCTTGAATT CCTTTCCTCC
    CATGACTCTT CAGTATCAGA AACAAATTCT GGGAATGGAA CTAACGTTTT ATTAGAAAGC
    TCACCACCAT CCCCATTCTT TCAGTTTCAA GGAGAAATGG TTGTAAATGA TTTGTATGTG
    CCATCATCAC CAGGCCTAAC ACCTATGAGA AGTCGTAGCA AGTTGGTTTC AAGACCATCT
    CGATCTTTTC TGCTAAGACA TCGGCCGGTA CCAGAGATTC CAGGATCAAC GAAGGGAATT
    TCAGGGAGCT ATTTCCGTGA GAGAAGAAAT GTTGCTACCT CAACTGGAAA ATCTGTGACA
    CAGCAAAATA CAAATGAGGA GAATTCATCT TCCATTCTAC CTTATGGAGA GACCTTTCTC
    CTCCAGAGAC TAGAAAATTC CAAGAAGAGA TCTATAAAGA ATGAATTTTG GACCCAGGAA
    GAAACACTCA AAGGGGAAAC AACTACTGAA AGAAACTCAT TTTTTGTCAA ATCAAGCATA
    TATGATAACA GGACGGAAAA TATAAGTGAA AACAAAGTAG AAGATATTTG GATACCTCGA
    GAGGACAAAA GCAGTCTTCC TATAGACTCT GCTTCAGAAT CAGAGTATTC AACAGTAGAA
    GAATGCTTTC AGAGTTTAAG AAGAAAAAAT TCAAAGCCAT CTAAATCAAG GACTCAAAAA
    GCAGCAAATT TGGACCCTGT TAATAGGCAC AGTTATCCAC TAAGCTCAAC AAGTGGAAAT
    GCTGATTCAC CAGTTATTTC CTCAAATGTA ATATCTCCCT ATGCCTGTTT TTATGGATCA
    TCTACAAGGA AGGTTAAATC CGGATGGCTT GACAAACTCT GTCCCCAAGG AAAACGCATG
    TTTCAGAAGA GATGGGTGAA ATTTGATGGC CTTAGCATTT CTTATTACAA TAATGATAAG
    GAAGAGAAGC TAAAACCCCC AAAATGGAAA ATAAGGAGAA GAAAAAGTTA TTCTGAGAAG
    TATTCAAAAG GAATAATCCC CCTTTCTGCT ATATCTACGG TACGAGTTCA AGGAGACAAC
    AAATTTGAAG TTGTTACAAC ACAAAGAACT TTTGTTTTTA GAGTAGAAAA AGAAGAGGAA
    AGAAATGACT GGATCAGCCT ACTATTAAAT GCACTGAAGT TACAGGCCCT TTCTTCGCAG
    TCTCAAGCTG CTATTGCACC TGAGAAATGT GGATATCTTG AACTGAGAGG CTATAAAGCT
    AAAATTTTTA CTGTGTTAAG TGGAAACAGT GTGTGGCTTT GCAAAAATGA ACAGGATTTT
    AAGAGTGGAC TTGGTATTAC CATAATCCCT ATGAATGTAG CAAATGTAAA ACAAGTGGAC
    CGAACTGTAA AACAGTCTTT TGAAATAATC ACTCCTTACA GGAGTTTTAG CTTTACAGCT
    GAGTCTGAAA AAGAGAAACA AGAGTGGATT GAAGCTGTGC AGCAATCAAT AGCAGAAACT
    CTCTCTGATT ATGAAGTAGC TGAGAAGATT TGGTACAATG AGTCCAACAG GAGCTGTGCA
    GATTGTAAAG CCCCAGATCC TGACTGGGCC TCCATCAATC TCTGTGTTGT CATCTGCAAG
    AAGTGTGCAG GGCAACATAG ATCTTTAGGA CCAAAAGATT CCAAGGTTAG AAGTCTGAAA
    ATGGATGCCA GCATTTGGAG CAATGAGCTC ATTGAGCTTT TCATTGTCAT TGGAAACAAA
    AGAGCTAATG ACTTTTGGGC TGGTAATCTT CAAAAAGATG AAGAATTACA CATGGACTGT
    TCAGTAGAAA AGAGAAAAAA TTTTATCACT CAGAAGTACA AAGAAGGAAG ATTCAGAAAA
    ACCCTTTTGG CGTCTCTCAC CAAGGAAGAA TTAAATAAGG CTCTGTGTGC TGCTGTGGTG
    AAACCAGATG TTCTGGAAAC AATGGCTTTG CTGTTCAGTG GAGCAGATGT CATGTGTGCA
    ACAGGTGATC CTGAGCACAG CACCCCCTAT CTGCTGGCCA AGAAGGCAGG GCAGAGTCTG
    CAGATGGAAT TTCTCTACCA TAACAAGTTC TCAGATTTCC CTCAACATGA CATATATTCT
    GAAGGTCGAT TACATCAAGA GTCCTCCCAG TCTACGTTCC TCTGTGACTT TTTGTATCAG
    GCTCCTGCTG GTGCTTCTAA ACTCTCTTCA GAAAAAAAAC TGCTTGAAGA GACAAATAAA
    AAGTGGTGTG TTCTAGAAGG AGGCTTCTTG AGTTACTATG AAAATGATAA GTCTACCACG
    CCTAATGGCA CCATTAATAT CAATGAAGTC ATCTGCCTGG CTGTGCACAA AGAAGACTTC
    TGTTTAAATA CTGGGCCCAT CTTTACTTTT GAGATTTATT TACCCTCAGA ACGTGCATTT
    TTATTTGGAG CTGAAACATC TCAAGTGCAA AGAAAATGGA CAGAAGCAAT AGCCAAGCAT
    TTTGTTCCCT TTGTTGCTGA AAACTTAACA GAAGCTGACT ATGATTTGAT TGGTCAACTC
    TACTACAAAG ACTGCCACGC CCTGGATCAG TGGAGAAAAG GCTGGTTTGC AATGGATAAA
    TCTAATTTGC GTTTTTGCCT TCAAATGCAA GAAGGTCAGG AGGATAGAAT GCACTTAAGA
    CGACTACAAG AACTAACAAT TAGCACAGTC ATTCAAAATG GGGAAAAATT GGATGTTTTG
    CTCTTGGTAG AAAAAGGGAG AACATTATAC ATCCATGGGC ATACCAAGTT GGATTTCACA
    GTCTGGCATA CTGCAATTGA AAAAGCAGCG GGTACAGATG GGAATGCATT ACAAGACCAG
    CAGCTCGGCA GAAATGACGT TCCCATTATC GTGAACAGCT GTATAGCATT TGTTACACAG
    TATGGTTTAG GATACAAAAA TATCTATCAA AAGAATGGTG ATCCATTACA TGTAAGTGAA
    CTCCTGGAGA GTTTCAAAAA GGATGCAAGA AGCTTTAAAT TGAGGGCTGG AAAACATCAA
    CTTGAAGATG TGACAGGTGT ATTGAAGAGT TTTCTCTCCG ACATTGATGA TGCGCTTCTC
    ACTAAGGAGC TCTACCCGTA TTGGATCTCT GCCTTAGATA CCCAAGATGA CAAGGAAAGA
    ATTAAGAAGT ATGGAGCATT TATACGTACT CTTCCTGGGG TGAACCGAGC AACATTGGCA
    GCTATAATTG AACACCTTTA CAGGGTTCAG AAATGCTCAG AAATCAATCA CATGAGTGCC
    CACAATTTGG CCTTGGTTTT TTCCTCCTGT TTCTTTCAAA CTAAGGGACA AACTAGTGAA
    GAAGTGAATG TAATCGAGGA CTTAATTAAT AATTATGTTG AAATTTTTGA GGTTAAAGAA
    GACCAAGTCA AACAAATGGA CATAGAGAAT AGCTTTATTA CCAAGTGGAA AGACACTCAA
    GTTTCCCAGG CTGGAGATTT GTTAATTGAA GTATATGTAG AAAGAAAGGA ACCTGACTGC
    AGTATTATAA TTCGGATATC TCCTGTGATG GAAGCAGAAG AGTTAACTAA TGACATATTA
    GCAATAAAAA ATATAATTCC AACAAAAGAT GATATCTGGG CCACATTTGA AGTCATAGAA
    AATGAAGAGC TCGAACGTCC TCTTCACTAC ACAGAAAATG TGTTGGAACA GGTGCTTCGA
    TGGAGTTCAT TAGCTGAACC TGGCTCTGCT TATCTTGTAG TGAAGAGATT CTTAACCATT
    GACACAATTA AACACTATAA TGAGAGGAAG GCCCTGGAAA ATATCAAAGA GGGAGTCTTG
    AAAGTTAAAG AAGAACCATC TAAAATACTC TCTGGAAATA AGTTTCAAGA TCGGTATTGT
    GTTTTACGAG ATGGGTACCT CCTTCTTTAC AAGGATCTGA AGCAAGGAAG CCTTCAGGTG
    GACATAAATA AATCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.