PIKFYVE gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025278129.1	XP_025133914.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278141.1	XP_025133926.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_006044598.1	XP_006044660.1	LOW QUALITY PROTEIN: 1-phosphatidylinositol 3-phosphate 5-kinase
XM_025278135.1	XP_025133920.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_025278131.1	XP_025133916.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278138.1	XP_025133923.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_025278139.1	XP_025133924.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_025278130.1	XP_025133915.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278132.1	XP_025133917.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278134.1	XP_025133919.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278142.1	XP_025133927.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_025278133.1	XP_025133918.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_025278136.1	XP_025133921.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_025278140.1	XP_025133925.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_025278143.1	XP_025133928.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
XM_025278144.1	XP_025133929.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu55867	XM_025278143.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu55868	XM_025278144.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBu03746	XM_006044598.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE finger containing (PIKFYVE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu55859	XM_025278131.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55859	XM_025278132.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55859	XM_025278130.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55859	XM_025278133.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55859	XM_025278134.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55859	XM_025278129.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55860	XM_025278135.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55861	XM_025278136.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55862	XM_025278138.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55863	XM_025278139.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55864	XM_025278140.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55865	XM_025278141.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu55866	XM_025278142.1 Latest version!	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

PIKFYVE ( XM_025278143.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133928.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBu55867
Clone ID Related Accession (Same CDS sequence)	XM_025278143.1
Accession Version	XM_025278143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCATTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133928.1
CDS	135..2507
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSF

Target ORF information:

RefSeq Version	XM_025278143.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278143.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCATTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278144.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133929.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBu55868
Clone ID Related Accession (Same CDS sequence)	XM_025278144.1
Accession Version	XM_025278144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA 
GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATACTG AGGAGATGGA GTGCCTGGTT 
GTTGTTGTTA TTTTCCCCCC CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133929.1
CDS	135..1901
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKDKKEHSLLRRSEIQDTEEMECLVVVVIFPPF

Target ORF information:

RefSeq Version	XM_025278144.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278144.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA 
GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATACTG AGGAGATGGA GTGCCTGGTT 
GTTGTTGTTA TTTTCCCCCC CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_006044598.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044660.1 Bubalus bubalis phosphoinositide kinase, FYVE finger containing (PIKFYVE), mRNA.

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CloneID	OBu03746
Clone ID Related Accession (Same CDS sequence)	XM_006044598.1
Accession Version	XM_006044598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: 1-phosphatidylinositol 3-phosphate 5-kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783639.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGATACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT 
GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC 
CGACTGTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGGAAATTT 
ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT 
TCCTCTGTGT CTGTACTTGA TCCAAGTGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC 
AGCCGCAACA TATTTTTAGA GGATGATTTT GCTTGGCAAA GTTTAATTCA TCCAGACTCC 
TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC ATCTCCAATG 
GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA 
CACCCCAATT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT 
GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT 
TGTGTCAGTC ACCATGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG 
AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA 
GCTGAAGAAG GTGACGATAA TTTGACTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCTCATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT 
GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA 
GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC 
CAACAGTTGC TCCATAGTGA GTCACTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT 
ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA 
AACCCCAAAA TTCTTCTGCT GAAGTGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG 
CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA 
CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTA 
GAACGAGTTA GCCGCATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC 
AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG 
CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTTCTTA TGGATGAATT TGCTATGCCT 
CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AGGGACAGGA GGATGAAGGG 
GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC 
TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAATCAAGGA TTCTGTTTGA GAAGGGTGAC 
CAGGAAAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA ACTCTTAAGA GCATGCCCCA 
GCAGCTTGCC CGGTGGGTGC CCCCTGCGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT 
CCGCTCCATG TGGATGACCA ACAGGATGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG 
CAAAAAGAGG AGCTTCCGGA TCCCGCAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG 
GATGACACCG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT 
GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC ACCAGTCATC 
ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC 
AGGCGGAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT 
CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG 
CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCTG ATTATCGGGC CAGAGGAGGA 
AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGTACCTCA 
AGTGGCAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACAAGTGAT 
GCAGTGTGGT CAACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCCA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC 
CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGCTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATCTGCAAA 
CAGGTAACAC CAGTTGTCGC TCTTTCCAAT GAGTCTTGGT CCATGTCATT TGCAAAATAC 
CTGGAACTTA GGTTTTATGG GCACCAGTAT ACCCGCAGAG CCAATGCTGA GCCATGTGGT 
CACTCCATCC ACCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCATCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA 
CGTCAAGTCC CATTAAAAGT GTCCCTTCTT CAGGATCTAA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTCGCGTCTT TGAAAACTGA TACATTTAGC 
AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGCGAG 
TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGAGACCCCC 
CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAACCT CTGTGAGGTG 
CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA 
GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC 
TTCTTAGCCA CCCTGTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT 
TCTCCTGAAG GTGTGCCTGA GCAGGCCATG GGAGGGCCCC CAGAACTCGA TACTGCCAGC 
AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA 
AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT 
CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG 
ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG 
GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG 
AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CAGATGCCAA CGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC 
AGTGAAGAAG ATTTCATTCG TTCGCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC 
AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT 
GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAAAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC CCAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT 
GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA 
AAGATGGTTC GAGACAACCC TCTGTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC 
TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA 
GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA 
TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CGACCGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG 
GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044660.1
CDS	30..6326
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLNS*EHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_006044598.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE finger containing (PIKFYVE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044598.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGATACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT 
GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC 
CGACTGTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGGAAATTT 
ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT 
TCCTCTGTGT CTGTACTTGA TCCAAGTGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC 
AGCCGCAACA TATTTTTAGA GGATGATTTT GCTTGGCAAA GTTTAATTCA TCCAGACTCC 
TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC ATCTCCAATG 
GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA 
CACCCCAATT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT 
GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT 
TGTGTCAGTC ACCATGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG 
AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA 
GCTGAAGAAG GTGACGATAA TTTGACTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCTCATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT 
GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA 
GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC 
CAACAGTTGC TCCATAGTGA GTCACTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT 
ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA 
AACCCCAAAA TTCTTCTGCT GAAGTGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG 
CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA 
CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTA 
GAACGAGTTA GCCGCATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC 
AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG 
CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTTCTTA TGGATGAATT TGCTATGCCT 
CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AGGGACAGGA GGATGAAGGG 
GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC 
TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAATCAAGGA TTCTGTTTGA GAAGGGTGAC 
CAGGAAAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA ACTCTTAAGA GCATGCCCCA 
GCAGCTTGCC CGGTGGGTGC CCCCTGCGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT 
CCGCTCCATG TGGATGACCA ACAGGATGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG 
CAAAAAGAGG AGCTTCCGGA TCCCGCAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG 
GATGACACCG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT 
GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC ACCAGTCATC 
ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC 
AGGCGGAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT 
CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG 
CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCTG ATTATCGGGC CAGAGGAGGA 
AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGTACCTCA 
AGTGGCAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACAAGTGAT 
GCAGTGTGGT CAACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCCA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC 
CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGCTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATCTGCAAA 
CAGGTAACAC CAGTTGTCGC TCTTTCCAAT GAGTCTTGGT CCATGTCATT TGCAAAATAC 
CTGGAACTTA GGTTTTATGG GCACCAGTAT ACCCGCAGAG CCAATGCTGA GCCATGTGGT 
CACTCCATCC ACCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCATCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA 
CGTCAAGTCC CATTAAAAGT GTCCCTTCTT CAGGATCTAA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTCGCGTCTT TGAAAACTGA TACATTTAGC 
AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGCGAG 
TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGAGACCCCC 
CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAACCT CTGTGAGGTG 
CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA 
GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC 
TTCTTAGCCA CCCTGTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT 
TCTCCTGAAG GTGTGCCTGA GCAGGCCATG GGAGGGCCCC CAGAACTCGA TACTGCCAGC 
AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA 
AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT 
CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG 
ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG 
GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG 
AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CAGATGCCAA CGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC 
AGTGAAGAAG ATTTCATTCG TTCGCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC 
AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT 
GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAAAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC CCAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT 
GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA 
AAGATGGTTC GAGACAACCC TCTGTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC 
TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA 
GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA 
TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CGACCGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG 
GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278129.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133914.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133914.1
CDS	135..6467
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278129.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278129.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278131.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133916.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133916.1
CDS	102..6434
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278131.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278131.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278130.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133915.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133915.1
CDS	1356..7688
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278130.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278130.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278132.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133917.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133917.1
CDS	160..6492
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278132.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278132.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278134.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133919.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133919.1
CDS	59..6391
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278134.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278134.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278133.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133918.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

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CloneID	OBu55859
Clone ID Related Accession (Same CDS sequence)	XM_025278129.1 , XM_025278131.1 , XM_025278130.1 , XM_025278132.1 , XM_025278134.1 , XM_025278133.1
Accession Version	XM_025278133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133918.1
CDS	128..6460
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278133.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278133.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGA GGACAAGGTA AAATGCCGAC CGTGGTCTCT 
CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA 
GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278135.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133920.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

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CloneID	OBu55860
Clone ID Related Accession (Same CDS sequence)	XM_025278135.1
Accession Version	XM_025278135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGATACC 
AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT 
GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG 
AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGCA ATCAAGAAAT CCCTGGGAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC 
TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAGT 
GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT 
TTTGCTTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGATC TGCATCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT 
CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ATTGCATTGT AGGAAAGGAG 
TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT 
GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCATGA CCAGCTCTTC 
AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT 
CCGGACAGTG ACTCGGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT 
AATTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCTCA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT 
TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT 
AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT 
AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCACTG 
TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT 
ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAGTGT 
TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG 
GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT 
ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTAGAACGAG TTAGCCGCAT GACCCAAGGT 
GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA 
TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA 
ATCTCCTTTC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC 
CATTCTCTGA TTGAGGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT 
CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG 
CTAGAATCAA GGATTCTGTT TGAGAAGGGT GACCAGGAAA ATAAGAGTGT GCCACAGGAT 
GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGGTGGG TGCCCCCTGC 
GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAT 
GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAAAAG AGGAGCTTCC GGATCCCGCA 
AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CCGGCCTGTA TGTTACCGAG 
GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG 
AAAGATGTGA TCCTCTGTAT CTCACCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT 
GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCAG AGCAGGTTTA CTGGTCTCCT 
CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGGA AGAAACAGAT GCTTAGGGAT 
CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC 
TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG 
AGAATGCTAG CTGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT 
TTTGCTTACT CAAAGGAGGC ATCAAGTACC TCAAGTGGCA AATCAGGAAG CAGAACTGAA 
GGTGATGAAG AAAAAGGGTT GATTACAAGT GATGCAGTGT GGTCAACAAA GGTGGACTGT 
CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA 
AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC 
CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTCACCT ACTCCTGGTG CAGAATCTGC AAACAGGTAA CACCAGTTGT CGCTCTTTCC 
AATGAGTCTT GGTCCATGTC ATTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG 
TATACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCCT ATAACCAGAT GGTGGCATCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA 
GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCATTAAA AGTGTCCCTT 
CTTCAGGATC TAAAGGACTT CTTTCAAAAA GTTTCACAGG TATATCTTGC TGTGGATGAA 
AGACTCGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT 
ATCTTTGCAC AAAAAGAGAT GGAAGAAAGC GAGTTCAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCC TGTCTTCCTC CATGGAGACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC 
CTCATCGCCA AGAAACAAAA CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA 
GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT 
CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTGTC CAGTCAGAGC 
TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGTGTGCC TGAGCAGGCC 
ATGGGAGGGC CCCCAGAACT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT 
TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA 
GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA 
CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG 
GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG 
ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG 
GAGCTTCAAT TTTCAGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG 
TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGTGAAG AAGATTTCAT TCGTTCGCTT 
TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT 
GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA 
TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAA 
AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCCCAGGTT 
TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC 
TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTGTAT 
ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT 
TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG 
CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACCGT GGTCTCTCCA 
GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC 
CACTGGACAG GCTTGGGTCT GAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133920.1
CDS	135..6464
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278135.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278135.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGATACC 
AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT 
GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG 
AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGCA ATCAAGAAAT CCCTGGGAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC 
TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAGT 
GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT 
TTTGCTTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGATC TGCATCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT 
CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ATTGCATTGT AGGAAAGGAG 
TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT 
GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCATGA CCAGCTCTTC 
AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT 
CCGGACAGTG ACTCGGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT 
AATTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCTCA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT 
TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT 
AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT 
AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCACTG 
TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT 
ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAGTGT 
TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG 
GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT 
ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTAGAACGAG TTAGCCGCAT GACCCAAGGT 
GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA 
TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA 
ATCTCCTTTC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC 
CATTCTCTGA TTGAGGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT 
CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG 
CTAGAATCAA GGATTCTGTT TGAGAAGGGT GACCAGGAAA ATAAGAGTGT GCCACAGGAT 
GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGGTGGG TGCCCCCTGC 
GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAT 
GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAAAAG AGGAGCTTCC GGATCCCGCA 
AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CCGGCCTGTA TGTTACCGAG 
GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG 
AAAGATGTGA TCCTCTGTAT CTCACCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT 
GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCAG AGCAGGTTTA CTGGTCTCCT 
CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGGA AGAAACAGAT GCTTAGGGAT 
CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC 
TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG 
AGAATGCTAG CTGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT 
TTTGCTTACT CAAAGGAGGC ATCAAGTACC TCAAGTGGCA AATCAGGAAG CAGAACTGAA 
GGTGATGAAG AAAAAGGGTT GATTACAAGT GATGCAGTGT GGTCAACAAA GGTGGACTGT 
CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA 
AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC 
CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTCACCT ACTCCTGGTG CAGAATCTGC AAACAGGTAA CACCAGTTGT CGCTCTTTCC 
AATGAGTCTT GGTCCATGTC ATTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG 
TATACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCATGA TTATCACCAG 
TATTTCTCCT ATAACCAGAT GGTGGCATCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA 
GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCATTAAA AGTGTCCCTT 
CTTCAGGATC TAAAGGACTT CTTTCAAAAA GTTTCACAGG TATATCTTGC TGTGGATGAA 
AGACTCGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT 
ATCTTTGCAC AAAAAGAGAT GGAAGAAAGC GAGTTCAAGA GCTGGACTGA GAAGATGCAG 
GCGCGGCTCC TGTCTTCCTC CATGGAGACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC 
CTCATCGCCA AGAAACAAAA CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA 
GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT 
CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTGTC CAGTCAGAGC 
TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGTGTGCC TGAGCAGGCC 
ATGGGAGGGC CCCCAGAACT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT 
TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA 
GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA 
CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG 
GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG 
ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG 
GAGCTTCAAT TTTCAGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG 
TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGTGAAG AAGATTTCAT TCGTTCGCTT 
TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT 
GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC 
TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA 
TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAA 
AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCCCAGGTT 
TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC 
TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTGTAT 
ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT 
TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG 
CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACCGT GGTCTCTCCA 
GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC 
CACTGGACAG GCTTGGGTCT GAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278136.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133921.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

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CloneID	OBu55861
Clone ID Related Accession (Same CDS sequence)	XM_025278136.1
Accession Version	XM_025278136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGATACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT 
GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC 
CGACTGTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGGAAATTT 
ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT 
TCCTCTGTGT CTGTACTTGA TCCAAGTGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC 
AGCCGCAACA TATTTTTAGA GGATGATTTT GCTTGGCAAA GTTTAATTCA TCCAGACTCC 
TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC ATCTCCAATG 
GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA 
CACCCCAATT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT 
GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT 
TGTGTCAGTC ACCATGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG 
AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA 
GCTGAAGAAG GTGACGATAA TTTGACTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCTCATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT 
GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA 
GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC 
CAACAGTTGC TCCATAGTGA GTCACTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT 
ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA 
AACCCCAAAA TTCTTCTGCT GAAGTGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG 
CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA 
CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTA 
GAACGAGTTA GCCGCATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC 
AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG 
CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTTCTTA TGGATGAATT TGCTATGCCT 
CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AGGGACAGGA GGATGAAGGG 
GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC 
TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAATCAAGGA TTCTGTTTGA GAAGGGTGAC 
CAGGAAAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA 
GCAGCTTGCC CGGTGGGTGC CCCCTGCGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT 
CCGCTCCATG TGGATGACCA ACAGGATGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG 
CAAAAAGAGG AGCTTCCGGA TCCCGCAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG 
GATGACACCG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT 
GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC ACCAGTCATC 
ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC 
AGGCGGAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT 
CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG 
CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCTG ATTATCGGGC CAGAGGAGGA 
AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGTACCTCA 
AGTGGCAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACAAGTGAT 
GCAGTGTGGT CAACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCCA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC 
CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGCTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATCTGCAAA 
CAGGTAACAC CAGTTGTCGC TCTTTCCAAT GAGTCTTGGT CCATGTCATT TGCAAAATAC 
CTGGAACTTA GGTTTTATGG GCACCAGTAT ACCCGCAGAG CCAATGCTGA GCCATGTGGT 
CACTCCATCC ACCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCATCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA 
CGTCAAGTCC CATTAAAAGT GTCCCTTCTT CAGGATCTAA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTCGCGTCTT TGAAAACTGA TACATTTAGC 
AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGCGAG 
TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGAGACCCCC 
CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAACCT CTGTGAGGTG 
CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA 
GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC 
TTCTTAGCCA CCCTGTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT 
TCTCCTGAAG GTGTGCCTGA GCAGGCCATG GGAGGGCCCC CAGAACTCGA TACTGCCAGC 
AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA 
AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT 
CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG 
ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG 
GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG 
AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CAGATGCCAA CGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC 
AGTGAAGAAG ATTTCATTCG TTCGCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC 
AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT 
GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAAAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC CCAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT 
GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA 
AAGATGGTTC GAGACAACCC TCTGTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC 
TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA 
GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA 
TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CGACCGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG 
GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133921.1
CDS	135..6431
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278136.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278136.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGATACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT 
GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC 
CGACTGTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGGAAATTT 
ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT 
TCCTCTGTGT CTGTACTTGA TCCAAGTGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC 
AGCCGCAACA TATTTTTAGA GGATGATTTT GCTTGGCAAA GTTTAATTCA TCCAGACTCC 
TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC ATCTCCAATG 
GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA 
CACCCCAATT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT 
GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT 
TGTGTCAGTC ACCATGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG 
AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA 
GCTGAAGAAG GTGACGATAA TTTGACTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCTCATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT 
GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA 
GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC 
CAACAGTTGC TCCATAGTGA GTCACTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT 
ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA 
AACCCCAAAA TTCTTCTGCT GAAGTGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG 
CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA 
CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTA 
GAACGAGTTA GCCGCATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC 
AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG 
CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTTCTTA TGGATGAATT TGCTATGCCT 
CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AGGGACAGGA GGATGAAGGG 
GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC 
TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAATCAAGGA TTCTGTTTGA GAAGGGTGAC 
CAGGAAAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA 
GCAGCTTGCC CGGTGGGTGC CCCCTGCGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT 
CCGCTCCATG TGGATGACCA ACAGGATGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG 
CAAAAAGAGG AGCTTCCGGA TCCCGCAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG 
GATGACACCG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT 
GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC ACCAGTCATC 
ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC 
AGGCGGAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT 
CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG 
CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCTG ATTATCGGGC CAGAGGAGGA 
AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGTACCTCA 
AGTGGCAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACAAGTGAT 
GCAGTGTGGT CAACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCCA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC 
CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGCTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATCTGCAAA 
CAGGTAACAC CAGTTGTCGC TCTTTCCAAT GAGTCTTGGT CCATGTCATT TGCAAAATAC 
CTGGAACTTA GGTTTTATGG GCACCAGTAT ACCCGCAGAG CCAATGCTGA GCCATGTGGT 
CACTCCATCC ACCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCATCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA 
CGTCAAGTCC CATTAAAAGT GTCCCTTCTT CAGGATCTAA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTCGCGTCTT TGAAAACTGA TACATTTAGC 
AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGCGAG 
TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGAGACCCCC 
CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAACCT CTGTGAGGTG 
CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA 
GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC 
TTCTTAGCCA CCCTGTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT 
TCTCCTGAAG GTGTGCCTGA GCAGGCCATG GGAGGGCCCC CAGAACTCGA TACTGCCAGC 
AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA 
AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT 
CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG 
ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG 
GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG 
AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CAGATGCCAA CGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC 
AGTGAAGAAG ATTTCATTCG TTCGCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC 
AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT 
GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAATT CTCAGAACAA CACTGAAAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTATGGGA GAAAGATGGC CCAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT 
GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA 
AAGATGGTTC GAGACAACCC TCTGTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC 
TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA 
GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA 
TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGAGGACAA 
GGTAAAATGC CGACCGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG 
GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278138.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133923.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

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CloneID	OBu55862
Clone ID Related Accession (Same CDS sequence)	XM_025278138.1
Accession Version	XM_025278138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGAGAGCTTT TACTGCTCAT CTCACCCCTG CAACAGATTA 
TATCCGAACA TTTACCTGGG ACAAAAAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133923.1
CDS	135..6347
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIESFYCSSHPCNRLYPNIYLGQKA

Target ORF information:

RefSeq Version	XM_025278138.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278138.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT 
GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT 
GAAAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ATGGGAGAAA GATGGCCCAG 
GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG 
AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTG 
TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC 
CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT 
GAGCTAGTAG TTGGAATTAT AGAGAGCTTT TACTGCTCAT CTCACCCCTG CAACAGATTA 
TATCCGAACA TTTACCTGGG ACAAAAAGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278139.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133924.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

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CloneID	OBu55863
Clone ID Related Accession (Same CDS sequence)	XM_025278139.1
Accession Version	XM_025278139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GGTTTTTGAC 
TTGAAGGGTT CACTTAGGAA TCGAAATGTA AAAACCGATA CCGGGAAAGA GAGCTGTGAT 
GTTGTTCTTC TGGATGAAAA TCTCTTAAAG ATGGTTCGAG ACAACCCTCT GTATATCCGT 
TCTCATTCCA AAGCTGTGCT GAGAGCCTCG ATCCGGAGCG ACTCCCACTT CCTTTCTAGC 
CACCTCATTA TAGACTATTC TTTGCTAGTT GGGCGAGACG ATACCAGCAA TGAGCTAGTA 
GTTGGAATTA TAGATTATAT CCGAACATTT ACCTGGGACA AAAAGCTTGA GATGGTTGTG 
AAATCAACAG GAATTTTAGG AGGACAAGGT AAAATGCCGA CCGTGGTCTC TCCAGAGTTG 
TACAGGACTA GATTCTGTGA AGCCATGGAC AAGTATTTCC TGATGGTGCC AGACCACTGG 
ACAGGCTTGG GTCTGAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133924.1
CDS	135..6338
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278139.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278139.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA 
GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT 
GTGGAGCTTC AATTTTCAGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGTG AAGAAGATTT CATTCGTTCG 
CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT 
ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT 
GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GGTTTTTGAC 
TTGAAGGGTT CACTTAGGAA TCGAAATGTA AAAACCGATA CCGGGAAAGA GAGCTGTGAT 
GTTGTTCTTC TGGATGAAAA TCTCTTAAAG ATGGTTCGAG ACAACCCTCT GTATATCCGT 
TCTCATTCCA AAGCTGTGCT GAGAGCCTCG ATCCGGAGCG ACTCCCACTT CCTTTCTAGC 
CACCTCATTA TAGACTATTC TTTGCTAGTT GGGCGAGACG ATACCAGCAA TGAGCTAGTA 
GTTGGAATTA TAGATTATAT CCGAACATTT ACCTGGGACA AAAAGCTTGA GATGGTTGTG 
AAATCAACAG GAATTTTAGG AGGACAAGGT AAAATGCCGA CCGTGGTCTC TCCAGAGTTG 
TACAGGACTA GATTCTGTGA AGCCATGGAC AAGTATTTCC TGATGGTGCC AGACCACTGG 
ACAGGCTTGG GTCTGAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278140.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133925.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

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CloneID	OBu55864
Clone ID Related Accession (Same CDS sequence)	XM_025278140.1
Accession Version	XM_025278140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA 
CTTACAAATC CTCATGTGGA GCTTCAATTT TCAGATGCCA ACGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGTGAAGAA 
GATTTCATTC GTTCGCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA 
GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG 
CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT 
TCTCAGAACA ACACTGAAAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTATGGG 
AGAAAGATGG CCCAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC 
GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT 
CGAGACAACC CTCTGTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG 
AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA 
GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGAGGACA AGGTAAAATG 
CCGACCGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT 
TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133925.1
CDS	135..6302
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTNSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPNEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278140.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278140.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC TCATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA 
TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA 
CTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG 
AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAG 
TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA 
CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC 
ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTAGAAC GAGTTAGCCG CATGACCCAA 
GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC 
AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA 
GAAATCTCCT TTCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC 
TTCCATTCTC TGATTGAGGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA 
AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT 
TTGCTAGAAT CAAGGATTCT GTTTGAGAAG GGTGACCAGG AAAATAAGAG TGTGCCACAG 
GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGGT GGGTGCCCCC 
TGCGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG 
GATGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA AAGAGGAGCT TCCGGATCCC 
GCAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACCGGCCT GTATGTTACC 
GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG 
TTGAAAGATG TGATCCTCTG TATCTCACCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA 
ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GGAAGAAACA GATGCTTAGG 
GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG 
CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG 
GGGAGAATGC TAGCTGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC 
CCTTTTGCTT ACTCAAAGGA GGCATCAAGT ACCTCAAGTG GCAAATCAGG AAGCAGAACT 
GAAGGTGATG AAGAAAAAGG GTTGATTACA AGTGATGCAG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG 
TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTCA CCTACTCCTG GTGCAGAATC TGCAAACAGG TAACACCAGT TGTCGCTCTT 
TCCAATGAGT CTTGGTCCAT GTCATTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC 
CAGTATACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCA TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTAAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATCT TGCTGTGGAT 
GAAAGACTCG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA 
GATATCTTTG CACAAAAAGA GATGGAAGAA AGCGAGTTCA AGAGCTGGAC TGAGAAGATG 
CAGGCGCGGC TCCTGTCTTC CTCCATGGAG ACCCCCCAGC AACTGCAGTC AGTCTTCGAG 
TCCCTCATCG CCAAGAAACA AAACCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG 
CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT 
TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT GTCCAGTCAG 
AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGTGT GCCTGAGCAG 
GCCATGGGAG GGCCCCCAGA ACTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA 
CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC 
AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC 
AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT 
GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA 
CTTACAAATC CTCATGTGGA GCTTCAATTT TCAGATGCCA ACGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGTGAAGAA 
GATTTCATTC GTTCGCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA 
GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG 
CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT 
TCTCAGAACA ACACTGAAAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTATGGG 
AGAAAGATGG CCCAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC 
GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT 
CGAGACAACC CTCTGTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG 
AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA 
GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGAGGACA AGGTAAAATG 
CCGACCGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT 
TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278141.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133926.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

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CloneID	OBu55865
Clone ID Related Accession (Same CDS sequence)	XM_025278141.1
Accession Version	XM_025278141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC 
AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC 
CATAGTGAGT CACTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGATTT 
GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT 
CTTCTGCTGA AGTGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT 
GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG 
CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTAGA ACGAGTTAGC 
CGCATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG 
GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAAC TAGAAATCTC CTTTCTTATG GATGAATTTG CTATGCCTCC TACATTAACG 
CAAAACCCTT CCTTCCATTC TCTGATTGAG GGACAGGAGG ATGAAGGGGC TTCACAGGAG 
CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT 
GATGATGGCA GTTTGCTAGA ATCAAGGATT CTGTTTGAGA AGGGTGACCA GGAAAATAAG 
AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG 
GTGGGTGCCC CCTGCGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG 
GATGACCAAC AGGATGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AAAAGAGGAG 
CTTCCGGATC CCGCAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACCGGC 
CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC 
TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCAC CAGTCATCAC ATTCCGGGAG 
CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGGAAGAAA 
CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC 
ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC 
AGCCAGAGCT TGGGGAGAAT GCTAGCTGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA 
AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GTACCTCAAG TGGCAAATCA 
GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CAAGTGATGC AGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG 
CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC 
TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGCTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TCTGCAAACA GGTAACACCA 
GTTGTCGCTC TTTCCAATGA GTCTTGGTCC ATGTCATTTG CAAAATACCT GGAACTTAGG 
TTTTATGGGC ACCAGTATAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CATCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTAAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
CTTGCTGTGG ATGAAAGACT CGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG 
GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGCGAGTT CAAGAGCTGG 
ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG AGACCCCCCA GCAACTGCAG 
TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAACCTCT GTGAGGTGCT GCAAGCTTGG 
AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT 
CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC 
CTGTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGT 
GTGCCTGAGC AGGCCATGGG AGGGCCCCCA GAACTCGATA CTGCCAGCAG TTCTGAAGAT 
GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG 
AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA 
GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT 
ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA 
CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG 
AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT 
ACAAATCCTC ATGTGGAGCT TCAATTTTCA GATGCCAACG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG TGAAGAAGAT 
TTCATTCGTT CGCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT 
GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA 
AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT 
CAGAACAACA CTGAAAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTATGGGAGA 
AAGATGGCCC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT 
ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA 
GACAACCCTC TGTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC 
GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC 
GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GAGGACAAGG TAAAATGCCG 
ACCGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC 
CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133926.1
CDS	135..6299
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLAEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTKYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278141.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278141.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAT 
ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT 
AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT 
GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGCT GCAATCAAGA AATCCCTGGG AAATTTATGG GTTATACAGG GGACCTCCGA 
GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA 
AGTGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT 
GATTTTGCTT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA 
CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG ATCTGCATCT CCAATGGTAC CATCATACGA GACGTCTGTC 
AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAATTGCAT TGTAGGAAAG 
GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA 
ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA TGACCAGCTC 
TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT 
TCTCCGGACA GTGACTCGGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG 
ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC 
AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC 
CATAGTGAGT CACTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGATTT 
GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT 
CTTCTGCTGA AGTGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT 
GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG 
CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTAGA ACGAGTTAGC 
CGCATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG 
GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAAC TAGAAATCTC CTTTCTTATG GATGAATTTG CTATGCCTCC TACATTAACG 
CAAAACCCTT CCTTCCATTC TCTGATTGAG GGACAGGAGG ATGAAGGGGC TTCACAGGAG 
CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT 
GATGATGGCA GTTTGCTAGA ATCAAGGATT CTGTTTGAGA AGGGTGACCA GGAAAATAAG 
AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG 
GTGGGTGCCC CCTGCGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG 
GATGACCAAC AGGATGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AAAAGAGGAG 
CTTCCGGATC CCGCAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACCGGC 
CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC 
TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCAC CAGTCATCAC ATTCCGGGAG 
CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGGAAGAAA 
CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC 
ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC 
AGCCAGAGCT TGGGGAGAAT GCTAGCTGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA 
AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GTACCTCAAG TGGCAAATCA 
GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CAAGTGATGC AGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG 
CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC 
TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGCTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TCTGCAAACA GGTAACACCA 
GTTGTCGCTC TTTCCAATGA GTCTTGGTCC ATGTCATTTG CAAAATACCT GGAACTTAGG 
TTTTATGGGC ACCAGTATAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CATCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTAAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
CTTGCTGTGG ATGAAAGACT CGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG 
GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGCGAGTT CAAGAGCTGG 
ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG AGACCCCCCA GCAACTGCAG 
TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAACCTCT GTGAGGTGCT GCAAGCTTGG 
AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT 
CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC 
CTGTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGT 
GTGCCTGAGC AGGCCATGGG AGGGCCCCCA GAACTCGATA CTGCCAGCAG TTCTGAAGAT 
GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG 
AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA 
GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT 
ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA 
CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG 
AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT 
ACAAATCCTC ATGTGGAGCT TCAATTTTCA GATGCCAACG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG TGAAGAAGAT 
TTCATTCGTT CGCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT 
GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA 
AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT 
CAGAACAACA CTGAAAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTATGGGAGA 
AAGATGGCCC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT 
ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA 
GACAACCCTC TGTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC 
GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC 
GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GAGGACAAGG TAAAATGCCG 
ACCGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC 
CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_025278142.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133927.1 Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

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CloneID	OBu55866
Clone ID Related Accession (Same CDS sequence)	XM_025278142.1
Accession Version	XM_025278142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGATACC 
AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT 
GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG 
AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGCA ATCAAGAAAT CCCTGGGAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC 
TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAGT 
GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT 
TTTGCTTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGATC TGCATCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT 
CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ATTGCATTGT AGGAAAGGAG 
TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT 
GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCATGA CCAGCTCTTC 
AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT 
CCGGACAGTG ACTCGGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT 
AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA 
GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT 
AGTGAGTCAC TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGATTTGTT 
TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT 
CTGCTGAAGT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT 
CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG 
GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTAGAACG AGTTAGCCGC 
ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC 
ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAACTAG AAATCTCCTT TCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA 
AACCCTTCCT TCCATTCTCT GATTGAGGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA 
TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT 
GATGGCAGTT TGCTAGAATC AAGGATTCTG TTTGAGAAGG GTGACCAGGA AAATAAGAGT 
GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGGTG 
GGTGCCCCCT GCGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT 
GACCAACAGG ATGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAA AGAGGAGCTT 
CCGGATCCCG CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACCGGCCTG 
TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT 
AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCACCAG TCATCACATT CCGGGAGCCC 
TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG GAAGAAACAG 
ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT 
CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC 
CAGAGCTTGG GGAGAATGCT AGCTGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA 
AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGTA CCTCAAGTGG CAAATCAGGA 
AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACAA GTGATGCAGT GTGGTCAACA 
AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT 
TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT 
GTCCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGC TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATCT GCAAACAGGT AACACCAGTT 
GTCGCTCTTT CCAATGAGTC TTGGTCCATG TCATTTGCAA AATACCTGGA ACTTAGGTTT 
TATGGGCACC AGTATACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCAT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTAAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATCTT 
GCTGTGGATG AAAGACTCGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA 
AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GCGAGTTCAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGAGA CCCCCCAGCA ACTGCAGTCA 
GTCTTCGAGT CCCTCATCGC CAAGAAACAA AACCTCTGTG AGGTGCTGCA AGCTTGGAAT 
AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA 
CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTG 
TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGTGTG 
CCTGAGCAGG CCATGGGAGG GCCCCCAGAA CTCGATACTG CCAGCAGTTC TGAAGATGTA 
TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG 
GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG 
GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT 
TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG 
ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT 
CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA 
AATCCTCATG TGGAGCTTCA ATTTTCAGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGTGA AGAAGATTTC 
ATTCGTTCGC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC 
TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG 
AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG 
AACAACACTG AAAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCCCAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC 
GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC 
AACCCTCTGT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC 
TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT 
ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCGACC 
GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG 
ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133927.1
CDS	135..6296
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSLSGSWTSPQLPSRTQSVRSPTPYKKRLNEEFQRRSSVLEDSLQHPQENSDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSASSVSVLDPSEPRTPVGSRKASRNIFLEDDFAWQSLIHPDSSNTALSTRLVSVQEDAGKSPARNRSASITNLSLDRSASPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLTKYLISDNGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSESLSTSWRDIIVSLVCQVVQTVRPDVKNRDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSSIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERVSRMTQGDLVMSMDQLLTKPHLGTCHKFYMQMFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLTQNPSFHSLIEGQEDEGASQEPFSGSPLPREPDFPPDFLPSDDGSLLESRILFEKGDQENKSVPQDVASLKPQEHAPAACPVGAPCALFPSVPESLLPLHVDDQQDAIGSEQPEALQQKEELPDPASQMRAFRDPLQDDTGLYVTEEVTSSEDKRKTDSLTFKQELKDVILCISPVITFREPFLLTDKGMRCSTRDYFAEQVYWSPLLNKEFKEMESRRKKQMLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQQKNSDPFAYSKEASSTSSGKSGSRTEGDEEKGLITSDAVWSTKVDCLNPVNHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQVPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKSREEKMEDIFAQKEMEESEFKSWTEKMQARLLSSSMETPQQLQSVFESLIAKKQNLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAVDASPRNASPGLPNGEKEDRFLATLSSQSSTSSPHLQLPTSPEGVPEQAMGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQRNSAEEGLPTNSTLDSRPKSSSPIRLPEISGGQTNRAVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGIEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRASIRSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_025278142.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278142.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
CCTACCAGTC CTTCTCATCT CACGCACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT 
CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA 
GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC 
CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGATACC 
AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT 
GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG 
AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGCA ATCAAGAAAT CCCTGGGAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC 
TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAGT 
GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT 
TTTGCTTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT 
GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGATC TGCATCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT 
CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ATTGCATTGT AGGAAAGGAG 
TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT 
GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCATGA CCAGCTCTTC 
AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT 
CCGGACAGTG ACTCGGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT 
AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA 
GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT 
AGTGAGTCAC TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGATTTGTT 
TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT 
CTGCTGAAGT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT 
CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG 
GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTAGAACG AGTTAGCCGC 
ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC 
ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAACTAG AAATCTCCTT TCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA 
AACCCTTCCT TCCATTCTCT GATTGAGGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA 
TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT 
GATGGCAGTT TGCTAGAATC AAGGATTCTG TTTGAGAAGG GTGACCAGGA AAATAAGAGT 
GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGGTG 
GGTGCCCCCT GCGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT 
GACCAACAGG ATGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAA AGAGGAGCTT 
CCGGATCCCG CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACCGGCCTG 
TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT 
AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCACCAG TCATCACATT CCGGGAGCCC 
TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG GAAGAAACAG 
ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT 
CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC 
CAGAGCTTGG GGAGAATGCT AGCTGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA 
AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGTA CCTCAAGTGG CAAATCAGGA 
AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACAA GTGATGCAGT GTGGTCAACA 
AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT 
TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT 
GTCCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGC TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATCT GCAAACAGGT AACACCAGTT 
GTCGCTCTTT CCAATGAGTC TTGGTCCATG TCATTTGCAA AATACCTGGA ACTTAGGTTT 
TATGGGCACC AGTATACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCAT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTAAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATCTT 
GCTGTGGATG AAAGACTCGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA 
AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GCGAGTTCAA GAGCTGGACT 
GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGAGA CCCCCCAGCA ACTGCAGTCA 
GTCTTCGAGT CCCTCATCGC CAAGAAACAA AACCTCTGTG AGGTGCTGCA AGCTTGGAAT 
AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA 
CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTG 
TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGTGTG 
CCTGAGCAGG CCATGGGAGG GCCCCCAGAA CTCGATACTG CCAGCAGTTC TGAAGATGTA 
TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG 
GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG 
GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT 
TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG 
ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT 
CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA 
AATCCTCATG TGGAGCTTCA ATTTTCAGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGTGA AGAAGATTTC 
ATTCGTTCGC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC 
TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG 
AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG 
AACAACACTG AAAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCCCAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC 
GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC 
AACCCTCTGT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC 
TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT 
ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCGACC 
GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG 
ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PIKFYVE
Entrez Gene ID	102413075
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PIKFYVE cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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