AMBRA1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006042386.1	XP_006042448.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
XM_006042389.1	XP_006042451.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X8
XM_006042391.1	XP_006042453.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X10
XM_006042385.2	XP_006042447.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_006042386.2	XP_006042448.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_006042388.2	XP_006042450.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
XM_006042389.2	XP_006042451.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
XM_006042387.1	XP_006042449.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
XM_006042390.1	XP_006042452.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X9
XM_006042391.2	XP_006042453.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X9
XM_006042382.1	XP_006042444.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_006042383.1	XP_006042445.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_006042383.2	XP_006042445.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_006042385.1	XP_006042447.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_006042384.1	XP_006042446.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_006042388.1	XP_006042450.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
XM_006042382.2	XP_006042444.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_006042384.2	XP_006042446.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_006042387.2	XP_006042449.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
XM_006042390.2	XP_006042452.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu01932	XM_006042383.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01932	XM_006042382.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01933	XM_006042384.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01934	XM_006042385.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01935	XM_006042386.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01936	XM_006042387.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01937	XM_006042388.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01938	XM_006042389.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01939	XM_006042390.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01940	XM_006042391.2 Latest version!	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBu01935	XM_006042386.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01938	XM_006042389.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01940	XM_006042391.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01932	XM_006042383.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01934	XM_006042385.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01937	XM_006042388.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01932	XM_006042382.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01933	XM_006042384.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01936	XM_006042387.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu01939	XM_006042390.1	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

AMBRA1 ( XM_006042382.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042444.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu01932
Clone ID Related Accession (Same CDS sequence)	XM_006042382.1 , XM_006042383.1 , XM_006042383.2 , XM_006042382.2
Accession Version	XM_006042382.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042444.1
CDS	303..4274
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042382.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042382.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042383.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042445.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu01932
Clone ID Related Accession (Same CDS sequence)	XM_006042382.1 , XM_006042383.1 , XM_006042383.2 , XM_006042382.2
Accession Version	XM_006042383.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042445.1
CDS	449..4420
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042383.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042383.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042383.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042445.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

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CloneID	OBu01932
Clone ID Related Accession (Same CDS sequence)	XM_006042382.1 , XM_006042383.1 , XM_006042383.2 , XM_006042382.2
Accession Version	XM_006042383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042445.1
CDS	306..4277
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042383.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042383.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042382.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042444.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

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CloneID	OBu01932
Clone ID Related Accession (Same CDS sequence)	XM_006042382.1 , XM_006042383.1 , XM_006042383.2 , XM_006042382.2
Accession Version	XM_006042382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042444.1
CDS	331..4302
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042382.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042382.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAAAAA 
ATATACCACT TGCAACTCTG TAACTCTCCG AAGACCTCAG GTGAAGAAAG CAGCCCCAGA 
TCACCTGGAG GCATGGCCTC AAACTCTGGT GTTGAGTACT ACTGGGACCA GCTGAACGAG 
ACGGTCTTCA CTGTTCACTC CAGCAGCCGA AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC 
ACGTGGAGGA CAGACAGAGA CATGGGCCTG ATGAATGCCA TCGGCCTGCA GCCCCGGAAC 
CCCACCACGT CGGTGACGTC TCAGGGCACC CAGACTCTGG CCCTGCAGCT GCAGAATGCG 
GAGACCCAGA CGGAGCGGGA GATACAGGAG CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA 
GGGGAGGGCT CGGACTACGG GGCCAGCGGG GAAGACGCGC TCAGCAGGAT CCAGCGGCTG 
ATGGCTGAAG GGGGCATGAC AGCCGTGGTG CAACGGGAGC AGAGCACCAC CATGGCCTCC 
ATGGGCGGCT TCGGAAACAA CATCATTGTC AGCCACCGCA TTCACCGCAG CTCCCAGACG 
GGCACGGAGC CGGGGGCCGT GCGCACCCCC TCGCCGCAGC CCTCCACCTC TCGGGGACTG 
CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA GGCTTGAGCC CCCGGACAGC CTCCTGGGAG 
CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT 
GCCCCCCTCC CCAGCACCGA GGGACCAACC CCGCCGCGCT GCGACCTGAC CAACAGCAAC 
CACCTTCCTG ATGGCGGGGG CAGCCGCAGG GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA 
CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042384.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042446.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

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CloneID	OBu01933
Clone ID Related Accession (Same CDS sequence)	XM_006042384.1 , XM_006042384.2
Accession Version	XM_006042384.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ACCCCACCAC GTCGGTGACG 
TCTCAGGGCA CCCAGACTCT GGCCCTGCAG CTGCAGAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACAGCCGTGG TGCAACGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AACATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GTGCGCACCC CCTCGCCGCA GCCCTCCACC TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG 
CTGGCAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCGGCCCGC TACCCCTGGG 
CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG GCCCCTCCTC CTGCCCCCCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGATGGCGGG 
GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042446.1
CDS	303..4196
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042384.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042384.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ACCCCACCAC GTCGGTGACG 
TCTCAGGGCA CCCAGACTCT GGCCCTGCAG CTGCAGAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACAGCCGTGG TGCAACGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AACATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GTGCGCACCC CCTCGCCGCA GCCCTCCACC TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG 
CTGGCAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCGGCCCGC TACCCCTGGG 
CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG GCCCCTCCTC CTGCCCCCCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGATGGCGGG 
GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042384.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042446.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu01933
Clone ID Related Accession (Same CDS sequence)	XM_006042384.1 , XM_006042384.2
Accession Version	XM_006042384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ACCCCACCAC GTCGGTGACG 
TCTCAGGGCA CCCAGACTCT GGCCCTGCAG CTGCAGAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACAGCCGTGG TGCAACGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AACATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GTGCGCACCC CCTCGCCGCA GCCCTCCACC TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG 
CTGGCAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCGGCCCGC TACCCCTGGG 
CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG GCCCCTCCTC CTGCCCCCCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGATGGCGGG 
GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042446.1
CDS	330..4223
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042384.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042384.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACGGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGACGCCA GCTGTGACGT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCACAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GATACGTGAT GGTGGGCTTG 
GCCTCACGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGCGG CGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCTGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ACCCCACCAC GTCGGTGACG 
TCTCAGGGCA CCCAGACTCT GGCCCTGCAG CTGCAGAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACAGCCGTGG TGCAACGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AACATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GTGCGCACCC CCTCGCCGCA GCCCTCCACC TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG 
CTGGCAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCGGCCCGC TACCCCTGGG 
CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG GCCCCTCCTC CTGCCCCCCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGATGGCGGG 
GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042385.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042447.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

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CloneID	OBu01934
Clone ID Related Accession (Same CDS sequence)	XM_006042385.2 , XM_006042385.1
Accession Version	XM_006042385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042447.1
CDS	331..4215
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042385.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042385.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042385.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042447.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu01934
Clone ID Related Accession (Same CDS sequence)	XM_006042385.2 , XM_006042385.1
Accession Version	XM_006042385.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042447.1
CDS	303..4187
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042385.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042385.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042386.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042448.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu01935
Clone ID Related Accession (Same CDS sequence)	XM_006042386.1 , XM_006042386.2
Accession Version	XM_006042386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCA GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCCCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCCCC 
GGGCTGACAG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCG 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTGCTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC CGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACCTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGTGCCTC CTGGCAGGTT CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA GCTCTACCAG CCCGCAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGTTCTGAGG AGGATTCTCT CAGGAGGAGG 
CTGCTGGAGT CTTCCCTCAT TTCATTATCC CGTTATGATG GAGCAGGATC CAGAGAGCAC 
CCAATTTACC CAGACCCAGC GAGATTATCT CCTGCTGCAT ACTATGCCCA GAGAATGATC 
CAGTACCTGT CACGGAGAGA CAGTATCCGC CAGCGCTCCA TGCGCTACCA GCAGAACCGC 
CTCCGTTCCT CCACCTCCTC CTCTTCCTCA GACAACCAGG GTCCATCCGT GGAGGGAACC 
GACTTGGAGT TTGAGGACTT TGAGGACGGT GGTGACAGAT CCAGGCACCG AGCTCCTCGC 
AATGCCCGCA TGTCTGCACC TTCGCTTGGA CGCTTTGTCC CAAGGCGTTT CTTGCTGCCT 
GAATACTTGC CTTATGCTGG GATTTTTCAT GAACGTGGAC AGCCTGGCTT GGCTACTCAC 
TCTTCTGTTA ACAGGGTCCT GGCAGGGGCA GTGATTGGTG ATGGACAGTC TGCCGTGGCC 
AGTAACATTG CCAATACTAC CTACCGGCTC CAGTGGTGGG ACTTCACTAA GTTTGACCTC 
CCGGAAATCA GTAATGCCTC CGTGAATGTG CTGGTGCAGA ACTGCAAGAT CTACAATGAC 
GCCAGCTGTG ACGTTTCTGC AGATGGCCAG CTCCTGGCAG CGTTCATCCC TAGCAGTCAG 
AGGGGCTTTC CTGACGAAGG CATCCTGGCA GTGTACTCCC TGGCCCCCCA CAACCTGGGC 
GAAATGCTCT ACACCAAGCG ATTTGGTCCC AACGCCATCT CAGTGAGCCT GTCCCCGATG 
GGCCGATACG TGATGGTGGG CTTGGCCTCA CGGAGGATCC TGCTGCACCC CTCCACGGAG 
CACATGGTGG CCCAGGTCTT CCGGCTGCAG CAGGCGCACG GCGGCGAGAC CTCCATGCGG 
AGAGTTTTCA ACGTCCTTTA CCCCATGCCT GCTGACCAGC GGAGACATGT CAGCATCAAT 
TCTGCCCGGT GGCTGCCTGA GCCAGGGCTT GGCCTGGCCT ACGGCACCAA CAAAGGAGAC 
CTGGTGATCT GCCGACCGGA AAAAATATAC CACTTGCAAC TCTGTAACTC TCCGAAGACC 
TCAGGTGAAG AAAGCAGCCC CAGATCACCT GGAGGCATGG CCTCAAACTC TGGTGTTGAG 
TACTACTGGG ACCAGCTGAA CGAGACGGTC TTCACTGTTC ACTCCAGCAG CCGAAGCAGC 
GAGAGGCCTG GAACCAGCAG AGCCACGTGG AGGACAGACA GAGACATGGG CCTGATGAAT 
GCCATCGGCC TGCAGCCCCG GAACCCCACC ACGTCGGTGA CGTCTCAGGG CACCCAGACT 
CTGGCCCTGC AGCTGCAGAA TGCGGAGACC CAGACGGAGC GGGAGATACA GGAGCCGGGG 
GTGGCGGCTT CAGGTCCTGG TGAAGGGGAG GGCTCGGACT ACGGGGCCAG CGGGGAAGAC 
GCGCTCAGCA GGATCCAGCG GCTGATGGCT GAAGGGGGCA TGACAGCCGT GGTGCAACGG 
GAGCAGAGCA CCACCATGGC CTCCATGGGC GGCTTCGGAA ACAACATCAT TGTCAGCCAC 
CGCATTCACC GCAGCTCCCA GACGGGCACG GAGCCGGGGG CCGTGCGCAC CCCCTCGCCG 
CAGCCCTCCA CCTCTCGGGG ACTGCTCCCG GAAGCCGGGC AGCTGGCAGA GCGAGGCTTG 
AGCCCCCGGA CAGCCTCCTG GGAGCGGCCC GCTACCCCTG GGCGGGAGTC CGCCCTGCCC 
TCCTCCTCCC CGGCCCCTCC TCCTGCCCCC CTCCCCAGCA CCGAGGGACC AACCCCGCCG 
CGCTGCGACC TGACCAACAG CAACCACCTT CCTGATGGCG GGGGCAGCCG CAGGGGGGAG 
GCGGCGGGCC CCAGCGGGGA GCCACGGGAC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042448.1
CDS	303..4118
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042386.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042386.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCA GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCCCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCCCC 
GGGCTGACAG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCG 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTGCTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC CGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACCTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGTGCCTC CTGGCAGGTT CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA GCTCTACCAG CCCGCAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGTTCTGAGG AGGATTCTCT CAGGAGGAGG 
CTGCTGGAGT CTTCCCTCAT TTCATTATCC CGTTATGATG GAGCAGGATC CAGAGAGCAC 
CCAATTTACC CAGACCCAGC GAGATTATCT CCTGCTGCAT ACTATGCCCA GAGAATGATC 
CAGTACCTGT CACGGAGAGA CAGTATCCGC CAGCGCTCCA TGCGCTACCA GCAGAACCGC 
CTCCGTTCCT CCACCTCCTC CTCTTCCTCA GACAACCAGG GTCCATCCGT GGAGGGAACC 
GACTTGGAGT TTGAGGACTT TGAGGACGGT GGTGACAGAT CCAGGCACCG AGCTCCTCGC 
AATGCCCGCA TGTCTGCACC TTCGCTTGGA CGCTTTGTCC CAAGGCGTTT CTTGCTGCCT 
GAATACTTGC CTTATGCTGG GATTTTTCAT GAACGTGGAC AGCCTGGCTT GGCTACTCAC 
TCTTCTGTTA ACAGGGTCCT GGCAGGGGCA GTGATTGGTG ATGGACAGTC TGCCGTGGCC 
AGTAACATTG CCAATACTAC CTACCGGCTC CAGTGGTGGG ACTTCACTAA GTTTGACCTC 
CCGGAAATCA GTAATGCCTC CGTGAATGTG CTGGTGCAGA ACTGCAAGAT CTACAATGAC 
GCCAGCTGTG ACGTTTCTGC AGATGGCCAG CTCCTGGCAG CGTTCATCCC TAGCAGTCAG 
AGGGGCTTTC CTGACGAAGG CATCCTGGCA GTGTACTCCC TGGCCCCCCA CAACCTGGGC 
GAAATGCTCT ACACCAAGCG ATTTGGTCCC AACGCCATCT CAGTGAGCCT GTCCCCGATG 
GGCCGATACG TGATGGTGGG CTTGGCCTCA CGGAGGATCC TGCTGCACCC CTCCACGGAG 
CACATGGTGG CCCAGGTCTT CCGGCTGCAG CAGGCGCACG GCGGCGAGAC CTCCATGCGG 
AGAGTTTTCA ACGTCCTTTA CCCCATGCCT GCTGACCAGC GGAGACATGT CAGCATCAAT 
TCTGCCCGGT GGCTGCCTGA GCCAGGGCTT GGCCTGGCCT ACGGCACCAA CAAAGGAGAC 
CTGGTGATCT GCCGACCGGA AAAAATATAC CACTTGCAAC TCTGTAACTC TCCGAAGACC 
TCAGGTGAAG AAAGCAGCCC CAGATCACCT GGAGGCATGG CCTCAAACTC TGGTGTTGAG 
TACTACTGGG ACCAGCTGAA CGAGACGGTC TTCACTGTTC ACTCCAGCAG CCGAAGCAGC 
GAGAGGCCTG GAACCAGCAG AGCCACGTGG AGGACAGACA GAGACATGGG CCTGATGAAT 
GCCATCGGCC TGCAGCCCCG GAACCCCACC ACGTCGGTGA CGTCTCAGGG CACCCAGACT 
CTGGCCCTGC AGCTGCAGAA TGCGGAGACC CAGACGGAGC GGGAGATACA GGAGCCGGGG 
GTGGCGGCTT CAGGTCCTGG TGAAGGGGAG GGCTCGGACT ACGGGGCCAG CGGGGAAGAC 
GCGCTCAGCA GGATCCAGCG GCTGATGGCT GAAGGGGGCA TGACAGCCGT GGTGCAACGG 
GAGCAGAGCA CCACCATGGC CTCCATGGGC GGCTTCGGAA ACAACATCAT TGTCAGCCAC 
CGCATTCACC GCAGCTCCCA GACGGGCACG GAGCCGGGGG CCGTGCGCAC CCCCTCGCCG 
CAGCCCTCCA CCTCTCGGGG ACTGCTCCCG GAAGCCGGGC AGCTGGCAGA GCGAGGCTTG 
AGCCCCCGGA CAGCCTCCTG GGAGCGGCCC GCTACCCCTG GGCGGGAGTC CGCCCTGCCC 
TCCTCCTCCC CGGCCCCTCC TCCTGCCCCC CTCCCCAGCA CCGAGGGACC AACCCCGCCG 
CGCTGCGACC TGACCAACAG CAACCACCTT CCTGATGGCG GGGGCAGCCG CAGGGGGGAG 
GCGGCGGGCC CCAGCGGGGA GCCACGGGAC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042386.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042448.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

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CloneID	OBu01935
Clone ID Related Accession (Same CDS sequence)	XM_006042386.1 , XM_006042386.2
Accession Version	XM_006042386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCA GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCCCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCCCC 
GGGCTGACAG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCG 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTGCTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC CGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACCTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGTGCCTC CTGGCAGGTT CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA GCTCTACCAG CCCGCAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGTTCTGAGG AGGATTCTCT CAGGAGGAGG 
CTGCTGGAGT CTTCCCTCAT TTCATTATCC CGTTATGATG GAGCAGGATC CAGAGAGCAC 
CCAATTTACC CAGACCCAGC GAGATTATCT CCTGCTGCAT ACTATGCCCA GAGAATGATC 
CAGTACCTGT CACGGAGAGA CAGTATCCGC CAGCGCTCCA TGCGCTACCA GCAGAACCGC 
CTCCGTTCCT CCACCTCCTC CTCTTCCTCA GACAACCAGG GTCCATCCGT GGAGGGAACC 
GACTTGGAGT TTGAGGACTT TGAGGACGGT GGTGACAGAT CCAGGCACCG AGCTCCTCGC 
AATGCCCGCA TGTCTGCACC TTCGCTTGGA CGCTTTGTCC CAAGGCGTTT CTTGCTGCCT 
GAATACTTGC CTTATGCTGG GATTTTTCAT GAACGTGGAC AGCCTGGCTT GGCTACTCAC 
TCTTCTGTTA ACAGGGTCCT GGCAGGGGCA GTGATTGGTG ATGGACAGTC TGCCGTGGCC 
AGTAACATTG CCAATACTAC CTACCGGCTC CAGTGGTGGG ACTTCACTAA GTTTGACCTC 
CCGGAAATCA GTAATGCCTC CGTGAATGTG CTGGTGCAGA ACTGCAAGAT CTACAATGAC 
GCCAGCTGTG ACGTTTCTGC AGATGGCCAG CTCCTGGCAG CGTTCATCCC TAGCAGTCAG 
AGGGGCTTTC CTGACGAAGG CATCCTGGCA GTGTACTCCC TGGCCCCCCA CAACCTGGGC 
GAAATGCTCT ACACCAAGCG ATTTGGTCCC AACGCCATCT CAGTGAGCCT GTCCCCGATG 
GGCCGATACG TGATGGTGGG CTTGGCCTCA CGGAGGATCC TGCTGCACCC CTCCACGGAG 
CACATGGTGG CCCAGGTCTT CCGGCTGCAG CAGGCGCACG GCGGCGAGAC CTCCATGCGG 
AGAGTTTTCA ACGTCCTTTA CCCCATGCCT GCTGACCAGC GGAGACATGT CAGCATCAAT 
TCTGCCCGGT GGCTGCCTGA GCCAGGGCTT GGCCTGGCCT ACGGCACCAA CAAAGGAGAC 
CTGGTGATCT GCCGACCGGA AAAAATATAC CACTTGCAAC TCTGTAACTC TCCGAAGACC 
TCAGGTGAAG AAAGCAGCCC CAGATCACCT GGAGGCATGG CCTCAAACTC TGGTGTTGAG 
TACTACTGGG ACCAGCTGAA CGAGACGGTC TTCACTGTTC ACTCCAGCAG CCGAAGCAGC 
GAGAGGCCTG GAACCAGCAG AGCCACGTGG AGGACAGACA GAGACATGGG CCTGATGAAT 
GCCATCGGCC TGCAGCCCCG GAACCCCACC ACGTCGGTGA CGTCTCAGGG CACCCAGACT 
CTGGCCCTGC AGCTGCAGAA TGCGGAGACC CAGACGGAGC GGGAGATACA GGAGCCGGGG 
GTGGCGGCTT CAGGTCCTGG TGAAGGGGAG GGCTCGGACT ACGGGGCCAG CGGGGAAGAC 
GCGCTCAGCA GGATCCAGCG GCTGATGGCT GAAGGGGGCA TGACAGCCGT GGTGCAACGG 
GAGCAGAGCA CCACCATGGC CTCCATGGGC GGCTTCGGAA ACAACATCAT TGTCAGCCAC 
CGCATTCACC GCAGCTCCCA GACGGGCACG GAGCCGGGGG CCGTGCGCAC CCCCTCGCCG 
CAGCCCTCCA CCTCTCGGGG ACTGCTCCCG GAAGCCGGGC AGCTGGCAGA GCGAGGCTTG 
AGCCCCCGGA CAGCCTCCTG GGAGCGGCCC GCTACCCCTG GGCGGGAGTC CGCCCTGCCC 
TCCTCCTCCC CGGCCCCTCC TCCTGCCCCC CTCCCCAGCA CCGAGGGACC AACCCCGCCG 
CGCTGCGACC TGACCAACAG CAACCACCTT CCTGATGGCG GGGGCAGCCG CAGGGGGGAG 
GCGGCGGGCC CCAGCGGGGA GCCACGGGAC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042448.1
CDS	331..4146
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042386.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042386.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCA GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCCCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCCCC 
GGGCTGACAG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCG 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTGCTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC CGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACCTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGTGCCTC CTGGCAGGTT CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA GCTCTACCAG CCCGCAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGTTCTGAGG AGGATTCTCT CAGGAGGAGG 
CTGCTGGAGT CTTCCCTCAT TTCATTATCC CGTTATGATG GAGCAGGATC CAGAGAGCAC 
CCAATTTACC CAGACCCAGC GAGATTATCT CCTGCTGCAT ACTATGCCCA GAGAATGATC 
CAGTACCTGT CACGGAGAGA CAGTATCCGC CAGCGCTCCA TGCGCTACCA GCAGAACCGC 
CTCCGTTCCT CCACCTCCTC CTCTTCCTCA GACAACCAGG GTCCATCCGT GGAGGGAACC 
GACTTGGAGT TTGAGGACTT TGAGGACGGT GGTGACAGAT CCAGGCACCG AGCTCCTCGC 
AATGCCCGCA TGTCTGCACC TTCGCTTGGA CGCTTTGTCC CAAGGCGTTT CTTGCTGCCT 
GAATACTTGC CTTATGCTGG GATTTTTCAT GAACGTGGAC AGCCTGGCTT GGCTACTCAC 
TCTTCTGTTA ACAGGGTCCT GGCAGGGGCA GTGATTGGTG ATGGACAGTC TGCCGTGGCC 
AGTAACATTG CCAATACTAC CTACCGGCTC CAGTGGTGGG ACTTCACTAA GTTTGACCTC 
CCGGAAATCA GTAATGCCTC CGTGAATGTG CTGGTGCAGA ACTGCAAGAT CTACAATGAC 
GCCAGCTGTG ACGTTTCTGC AGATGGCCAG CTCCTGGCAG CGTTCATCCC TAGCAGTCAG 
AGGGGCTTTC CTGACGAAGG CATCCTGGCA GTGTACTCCC TGGCCCCCCA CAACCTGGGC 
GAAATGCTCT ACACCAAGCG ATTTGGTCCC AACGCCATCT CAGTGAGCCT GTCCCCGATG 
GGCCGATACG TGATGGTGGG CTTGGCCTCA CGGAGGATCC TGCTGCACCC CTCCACGGAG 
CACATGGTGG CCCAGGTCTT CCGGCTGCAG CAGGCGCACG GCGGCGAGAC CTCCATGCGG 
AGAGTTTTCA ACGTCCTTTA CCCCATGCCT GCTGACCAGC GGAGACATGT CAGCATCAAT 
TCTGCCCGGT GGCTGCCTGA GCCAGGGCTT GGCCTGGCCT ACGGCACCAA CAAAGGAGAC 
CTGGTGATCT GCCGACCGGA AAAAATATAC CACTTGCAAC TCTGTAACTC TCCGAAGACC 
TCAGGTGAAG AAAGCAGCCC CAGATCACCT GGAGGCATGG CCTCAAACTC TGGTGTTGAG 
TACTACTGGG ACCAGCTGAA CGAGACGGTC TTCACTGTTC ACTCCAGCAG CCGAAGCAGC 
GAGAGGCCTG GAACCAGCAG AGCCACGTGG AGGACAGACA GAGACATGGG CCTGATGAAT 
GCCATCGGCC TGCAGCCCCG GAACCCCACC ACGTCGGTGA CGTCTCAGGG CACCCAGACT 
CTGGCCCTGC AGCTGCAGAA TGCGGAGACC CAGACGGAGC GGGAGATACA GGAGCCGGGG 
GTGGCGGCTT CAGGTCCTGG TGAAGGGGAG GGCTCGGACT ACGGGGCCAG CGGGGAAGAC 
GCGCTCAGCA GGATCCAGCG GCTGATGGCT GAAGGGGGCA TGACAGCCGT GGTGCAACGG 
GAGCAGAGCA CCACCATGGC CTCCATGGGC GGCTTCGGAA ACAACATCAT TGTCAGCCAC 
CGCATTCACC GCAGCTCCCA GACGGGCACG GAGCCGGGGG CCGTGCGCAC CCCCTCGCCG 
CAGCCCTCCA CCTCTCGGGG ACTGCTCCCG GAAGCCGGGC AGCTGGCAGA GCGAGGCTTG 
AGCCCCCGGA CAGCCTCCTG GGAGCGGCCC GCTACCCCTG GGCGGGAGTC CGCCCTGCCC 
TCCTCCTCCC CGGCCCCTCC TCCTGCCCCC CTCCCCAGCA CCGAGGGACC AACCCCGCCG 
CGCTGCGACC TGACCAACAG CAACCACCTT CCTGATGGCG GGGGCAGCCG CAGGGGGGAG 
GCGGCGGGCC CCAGCGGGGA GCCACGGGAC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042387.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042449.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu01936
Clone ID Related Accession (Same CDS sequence)	XM_006042387.1 , XM_006042387.2
Accession Version	XM_006042387.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAACCCCAC CACGTCGGTG ACGTCTCAGG GCACCCAGAC TCTGGCCCTG 
CAGCTGCAGA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACAGCCG TGGTGCAACG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAACATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC GGAAGCCGGG CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCGGCC CGCTACCCCT GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC 
CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGATGGC GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042449.1
CDS	303..4109
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042387.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042387.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAACCCCAC CACGTCGGTG ACGTCTCAGG GCACCCAGAC TCTGGCCCTG 
CAGCTGCAGA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACAGCCG TGGTGCAACG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAACATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC GGAAGCCGGG CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCGGCC CGCTACCCCT GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC 
CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGATGGC GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042387.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042449.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu01936
Clone ID Related Accession (Same CDS sequence)	XM_006042387.1 , XM_006042387.2
Accession Version	XM_006042387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAACCCCAC CACGTCGGTG ACGTCTCAGG GCACCCAGAC TCTGGCCCTG 
CAGCTGCAGA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACAGCCG TGGTGCAACG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAACATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC GGAAGCCGGG CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCGGCC CGCTACCCCT GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC 
CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGATGGC GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042449.1
CDS	330..4136
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042387.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042387.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAACCCCAC CACGTCGGTG ACGTCTCAGG GCACCCAGAC TCTGGCCCTG 
CAGCTGCAGA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACAGCCG TGGTGCAACG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAACATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC GGAAGCCGGG CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCGGCC CGCTACCCCT GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC 
CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGATGGC GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042388.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042450.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

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CloneID	OBu01937
Clone ID Related Accession (Same CDS sequence)	XM_006042388.2 , XM_006042388.1
Accession Version	XM_006042388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042450.1
CDS	332..4036
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042388.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042388.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042388.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042450.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

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CloneID	OBu01937
Clone ID Related Accession (Same CDS sequence)	XM_006042388.2 , XM_006042388.1
Accession Version	XM_006042388.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042450.1
CDS	303..4007
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042388.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042388.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCAGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCCCCGGGC TGACAGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCGGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG TGCCTCCTGG 
CAGGTTCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAGCTC TACCAGCCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG AGGGACGGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGACG CCAGCTGTGA CGTTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAC 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGATACGT GATGGTGGGC TTGGCCTCAC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG CGGCGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCTGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAA AAAATATACC ACTTGCAACT CTGTAACTCT 
CCGAAGACCT CAGGTGAAGA AAGCAGCCCC AGATCACCTG GAGGCATGGC CTCAAACTCT 
GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT TCACTGTTCA CTCCAGCAGC 
CGAAGCAGCG AGAGGCCTGG AACCAGCAGA GCCACGTGGA GGACAGACAG AGACATGGGC 
CTGATGAATG CCATCGGCCT GCAGCCCCGG AACCCCACCA CGTCGGTGAC GTCTCAGGGC 
ACCCAGACTC TGGCCCTGCA GCTGCAGAAT GCGGAGACCC AGACGGAGCG GGAGATACAG 
GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT GAAGGGGAGG GCTCGGACTA CGGGGCCAGC 
GGGGAAGACG CGCTCAGCAG GATCCAGCGG CTGATGGCTG AAGGGGGCAT GACAGCCGTG 
GTGCAACGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG GCTTCGGAAA CAACATCATT 
GTCAGCCACC GCATTCACCG CAGCTCCCAG ACGGGCACGG AGCCGGGGGC CGTGCGCACC 
CCCTCGCCGC AGCCCTCCAC CTCTCGGGGA CTGCTCCCGG AAGCCGGGCA GCTGGCAGAG 
CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG GAGCGGCCCG CTACCCCTGG GCGGGAGTCC 
GCCCTGCCCT CCTCCTCCCC GGCCCCTCCT CCTGCCCCCC TCCCCAGCAC CGAGGGACCA 
ACCCCGCCGC GCTGCGACCT GACCAACAGC AACCACCTTC CTGATGGCGG GGGCAGCCGC 
AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042389.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042451.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu01938
Clone ID Related Accession (Same CDS sequence)	XM_006042389.1 , XM_006042389.2
Accession Version	XM_006042389.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAAAAA ATATACCACT TGCAACTCTG TAACTCTCCG 
AAGACCTCAG GTGAAGAAAG CAGCCCCAGA TCACCTGGAG GCATGGCCTC AAACTCTGGT 
GTTGAGTACT ACTGGGACCA GCTGAACGAG ACGGTCTTCA CTGTTCACTC CAGCAGCCGA 
AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC ACGTGGAGGA CAGACAGAGA CATGGGCCTG 
ATGAATGCCA TCGGCCTGCA GCCCCGGAAC CCCACCACGT CGGTGACGTC TCAGGGCACC 
CAGACTCTGG CCCTGCAGCT GCAGAATGCG GAGACCCAGA CGGAGCGGGA GATACAGGAG 
CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA GGGGAGGGCT CGGACTACGG GGCCAGCGGG 
GAAGACGCGC TCAGCAGGAT CCAGCGGCTG ATGGCTGAAG GGGGCATGAC AGCCGTGGTG 
CAACGGGAGC AGAGCACCAC CATGGCCTCC ATGGGCGGCT TCGGAAACAA CATCATTGTC 
AGCCACCGCA TTCACCGCAG CTCCCAGACG GGCACGGAGC CGGGGGCCGT GCGCACCCCC 
TCGCCGCAGC CCTCCACCTC TCGGGGACTG CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA 
GGCTTGAGCC CCCGGACAGC CTCCTGGGAG CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC 
CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT GCCCCCCTCC CCAGCACCGA GGGACCAACC 
CCGCCGCGCT GCGACCTGAC CAACAGCAAC CACCTTCCTG ATGGCGGGGG CAGCCGCAGG 
GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042451.1
CDS	303..4004
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042389.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042389.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAAAAA ATATACCACT TGCAACTCTG TAACTCTCCG 
AAGACCTCAG GTGAAGAAAG CAGCCCCAGA TCACCTGGAG GCATGGCCTC AAACTCTGGT 
GTTGAGTACT ACTGGGACCA GCTGAACGAG ACGGTCTTCA CTGTTCACTC CAGCAGCCGA 
AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC ACGTGGAGGA CAGACAGAGA CATGGGCCTG 
ATGAATGCCA TCGGCCTGCA GCCCCGGAAC CCCACCACGT CGGTGACGTC TCAGGGCACC 
CAGACTCTGG CCCTGCAGCT GCAGAATGCG GAGACCCAGA CGGAGCGGGA GATACAGGAG 
CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA GGGGAGGGCT CGGACTACGG GGCCAGCGGG 
GAAGACGCGC TCAGCAGGAT CCAGCGGCTG ATGGCTGAAG GGGGCATGAC AGCCGTGGTG 
CAACGGGAGC AGAGCACCAC CATGGCCTCC ATGGGCGGCT TCGGAAACAA CATCATTGTC 
AGCCACCGCA TTCACCGCAG CTCCCAGACG GGCACGGAGC CGGGGGCCGT GCGCACCCCC 
TCGCCGCAGC CCTCCACCTC TCGGGGACTG CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA 
GGCTTGAGCC CCCGGACAGC CTCCTGGGAG CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC 
CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT GCCCCCCTCC CCAGCACCGA GGGACCAACC 
CCGCCGCGCT GCGACCTGAC CAACAGCAAC CACCTTCCTG ATGGCGGGGG CAGCCGCAGG 
GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042389.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042451.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

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CloneID	OBu01938
Clone ID Related Accession (Same CDS sequence)	XM_006042389.1 , XM_006042389.2
Accession Version	XM_006042389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAAAAA ATATACCACT TGCAACTCTG TAACTCTCCG 
AAGACCTCAG GTGAAGAAAG CAGCCCCAGA TCACCTGGAG GCATGGCCTC AAACTCTGGT 
GTTGAGTACT ACTGGGACCA GCTGAACGAG ACGGTCTTCA CTGTTCACTC CAGCAGCCGA 
AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC ACGTGGAGGA CAGACAGAGA CATGGGCCTG 
ATGAATGCCA TCGGCCTGCA GCCCCGGAAC CCCACCACGT CGGTGACGTC TCAGGGCACC 
CAGACTCTGG CCCTGCAGCT GCAGAATGCG GAGACCCAGA CGGAGCGGGA GATACAGGAG 
CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA GGGGAGGGCT CGGACTACGG GGCCAGCGGG 
GAAGACGCGC TCAGCAGGAT CCAGCGGCTG ATGGCTGAAG GGGGCATGAC AGCCGTGGTG 
CAACGGGAGC AGAGCACCAC CATGGCCTCC ATGGGCGGCT TCGGAAACAA CATCATTGTC 
AGCCACCGCA TTCACCGCAG CTCCCAGACG GGCACGGAGC CGGGGGCCGT GCGCACCCCC 
TCGCCGCAGC CCTCCACCTC TCGGGGACTG CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA 
GGCTTGAGCC CCCGGACAGC CTCCTGGGAG CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC 
CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT GCCCCCCTCC CCAGCACCGA GGGACCAACC 
CCGCCGCGCT GCGACCTGAC CAACAGCAAC CACCTTCCTG ATGGCGGGGG CAGCCGCAGG 
GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042451.1
CDS	330..4031
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEKIYHLQLCNSPKTSGEESSPRSPGGMASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042389.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042389.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAAAAA ATATACCACT TGCAACTCTG TAACTCTCCG 
AAGACCTCAG GTGAAGAAAG CAGCCCCAGA TCACCTGGAG GCATGGCCTC AAACTCTGGT 
GTTGAGTACT ACTGGGACCA GCTGAACGAG ACGGTCTTCA CTGTTCACTC CAGCAGCCGA 
AGCAGCGAGA GGCCTGGAAC CAGCAGAGCC ACGTGGAGGA CAGACAGAGA CATGGGCCTG 
ATGAATGCCA TCGGCCTGCA GCCCCGGAAC CCCACCACGT CGGTGACGTC TCAGGGCACC 
CAGACTCTGG CCCTGCAGCT GCAGAATGCG GAGACCCAGA CGGAGCGGGA GATACAGGAG 
CCGGGGGTGG CGGCTTCAGG TCCTGGTGAA GGGGAGGGCT CGGACTACGG GGCCAGCGGG 
GAAGACGCGC TCAGCAGGAT CCAGCGGCTG ATGGCTGAAG GGGGCATGAC AGCCGTGGTG 
CAACGGGAGC AGAGCACCAC CATGGCCTCC ATGGGCGGCT TCGGAAACAA CATCATTGTC 
AGCCACCGCA TTCACCGCAG CTCCCAGACG GGCACGGAGC CGGGGGCCGT GCGCACCCCC 
TCGCCGCAGC CCTCCACCTC TCGGGGACTG CTCCCGGAAG CCGGGCAGCT GGCAGAGCGA 
GGCTTGAGCC CCCGGACAGC CTCCTGGGAG CGGCCCGCTA CCCCTGGGCG GGAGTCCGCC 
CTGCCCTCCT CCTCCCCGGC CCCTCCTCCT GCCCCCCTCC CCAGCACCGA GGGACCAACC 
CCGCCGCGCT GCGACCTGAC CAACAGCAAC CACCTTCCTG ATGGCGGGGG CAGCCGCAGG 
GGGGAGGCGG CGGGCCCCAG CGGGGAGCCA CGGGACAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042390.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042452.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

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CloneID	OBu01939
Clone ID Related Accession (Same CDS sequence)	XM_006042390.1 , XM_006042390.2
Accession Version	XM_006042390.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ACCCCACCAC GTCGGTGACG TCTCAGGGCA CCCAGACTCT GGCCCTGCAG 
CTGCAGAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACAGCCGTGG TGCAACGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AACATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GTGCGCACCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG CTGGCAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCGGCCCGC TACCCCTGGG CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG 
GCCCCTCCTC CTGCCCCCCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGATGGCGGG GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042452.1
CDS	303..3926
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042390.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042390.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ACCCCACCAC GTCGGTGACG TCTCAGGGCA CCCAGACTCT GGCCCTGCAG 
CTGCAGAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACAGCCGTGG TGCAACGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AACATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GTGCGCACCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG CTGGCAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCGGCCCGC TACCCCTGGG CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG 
GCCCCTCCTC CTGCCCCCCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGATGGCGGG GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042390.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042452.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

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CloneID	OBu01939
Clone ID Related Accession (Same CDS sequence)	XM_006042390.1 , XM_006042390.2
Accession Version	XM_006042390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ACCCCACCAC GTCGGTGACG TCTCAGGGCA CCCAGACTCT GGCCCTGCAG 
CTGCAGAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACAGCCGTGG TGCAACGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AACATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GTGCGCACCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG CTGGCAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCGGCCCGC TACCCCTGGG CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG 
GCCCCTCCTC CTGCCCCCCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGATGGCGGG GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042452.1
CDS	329..3952
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042390.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042390.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACGGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGACGCCA GCTGTGACGT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCACAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GATACGTGAT GGTGGGCTTG GCCTCACGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGCGG CGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCTGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ACCCCACCAC GTCGGTGACG TCTCAGGGCA CCCAGACTCT GGCCCTGCAG 
CTGCAGAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACAGCCGTGG TGCAACGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AACATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GTGCGCACCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCGGA AGCCGGGCAG CTGGCAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCGGCCCGC TACCCCTGGG CGGGAGTCCG CCCTGCCCTC CTCCTCCCCG 
GCCCCTCCTC CTGCCCCCCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGATGGCGGG GGCAGCCGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042391.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042453.1 Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X10, mRNA.

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CloneID	OBu01940
Clone ID Related Accession (Same CDS sequence)	XM_006042391.1 , XM_006042391.2
Accession Version	XM_006042391.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782449.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACGGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGACG CCAGCTGTGA CGTTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAC AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGATACGT GATGGTGGGC 
TTGGCCTCAC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG CGGCGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCTGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAACCCCAC CACGTCGGTG 
ACGTCTCAGG GCACCCAGAC TCTGGCCCTG CAGCTGCAGA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACAGCCG TGGTGCAACG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAACATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG GACTGCTCCC GGAAGCCGGG 
CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCGGCC CGCTACCCCT 
GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGATGGC 
GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042453.1
CDS	303..3839
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042391.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042391.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACGGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGACG CCAGCTGTGA CGTTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAC AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGATACGT GATGGTGGGC 
TTGGCCTCAC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG CGGCGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCTGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAACCCCAC CACGTCGGTG 
ACGTCTCAGG GCACCCAGAC TCTGGCCCTG CAGCTGCAGA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACAGCCG TGGTGCAACG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAACATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG GACTGCTCCC GGAAGCCGGG 
CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCGGCC CGCTACCCCT 
GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGATGGC 
GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006042391.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006042453.1 Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X10, mRNA.

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CloneID	OBu01940
Clone ID Related Accession (Same CDS sequence)	XM_006042391.1 , XM_006042391.2
Accession Version	XM_006042391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006042391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACGGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGACG CCAGCTGTGA CGTTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAC AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGATACGT GATGGTGGGC 
TTGGCCTCAC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG CGGCGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCTGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAACCCCAC CACGTCGGTG 
ACGTCTCAGG GCACCCAGAC TCTGGCCCTG CAGCTGCAGA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACAGCCG TGGTGCAACG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAACATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG GACTGCTCCC GGAAGCCGGG 
CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCGGCC CGCTACCCCT 
GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGATGGC 
GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006042453.1
CDS	329..3865
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNPIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTANEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPADGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPPAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGSSTSPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDGGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDVSADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAVRTPSPQPSTSRGLLPEAGQLAERGLSPRTASWERPATPGRESALPSSSPAPPPAPLPSTEGPTPPRCDLTNSNHLPDGGGSRRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_006042391.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006042391.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCTGTC CGGATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACCTTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCA GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCATGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATCCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC TAACGAGATC 
CACTTCTGGG ACTGGAGCCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC GAACGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGCAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCTCAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCAGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCCCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCCCCGGGC TGACAGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCGGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT GCTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCCGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG TGCCTCCTGG CAGGTTCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAGCTC TACCAGCCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGTT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACGGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGACG CCAGCTGTGA CGTTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAC AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGATACGT GATGGTGGGC 
TTGGCCTCAC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG CGGCGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCTGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAACCCCAC CACGTCGGTG 
ACGTCTCAGG GCACCCAGAC TCTGGCCCTG CAGCTGCAGA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACAGCCG TGGTGCAACG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAACATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGTGCGCA CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG GACTGCTCCC GGAAGCCGGG 
CAGCTGGCAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCGGCC CGCTACCCCT 
GGGCGGGAGT CCGCCCTGCC CTCCTCCTCC CCGGCCCCTC CTCCTGCCCC CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGATGGC 
GGGGGCAGCC GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AMBRA1
Entrez Gene ID	102409652
Full Name	autophagy and beclin 1 regulator 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AMBRA1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

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