COL28A1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL28A1
Entrez Gene ID	102407731
Full Name	collagen type XXVIII alpha 1 chain
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006074585.1	XP_006074647.1	collagen alpha-1(XXVIII) chain
XM_006074585.2	XP_006074647.1	collagen alpha-1(XXVIII) chain

Pathways from BioSystems
Gene Ontology

The following COL28A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL28A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu28456	XM_006074585.1 Latest version!	Bubalus bubalis collagen, type XXVIII, alpha 1 (COL28A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu70559	XM_006074585.2 Latest version!	Bubalus bubalis collagen type XXVIII alpha 1 chain (COL28A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL28A1 ( XM_006074585.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006074647.1 Bubalus bubalis collagen, type XXVIII, alpha 1 (COL28A1), mRNA.

Price & Availability↑

CloneID	OBu28456
Clone ID Related Accession (Same CDS sequence)	XM_006074585.1
Accession Version	XM_006074585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785597.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GACATTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC GTTGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTCTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTATT ATGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCGTGAAAG TGACTGTGCT GATGATGGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGATGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ATGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAACGTAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAG GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGTGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGGCCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATTGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCTCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GGCCTCCAGG ACCTACTGGT 
GTAGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGACCAAAG GGTGAAAAAG GTTCTCAAGG ACCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATACGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TAGGACTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCAGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGCGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGGCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGC 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCCGGACC CCAAGGAGAA 
CAGGGGTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGGCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CGGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCATAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTGACTCAGT TCTCCAGCAA GGATGACCTC 
AAGCAGGCTG TGGACGACAT GCAGTATCTG GGGGAAGGTA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT CGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACCGATG GGCAGACAGA TACCCGAGAC AAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG CGAGCATGTT 
TATCTATTTG ATGACTTCAT TACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCATCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CCGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TACATGTCTC CGGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGACTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGGT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTATGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGTGA AAAGGAGTGT 
GAAGAAGTCT GCATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006074647.1
CDS	172..3615
Translation	MWNRHFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFVVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGVKVTVLMMDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPGGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGPIGPQGEAGPSIKGDKGDTGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKGDEGKKGSKGNQGQKGSQGPGGPKGDPGIMGPVGMPGISIPGPPGPKGDRGGPGMPGFKGEPGIAIRGPKGAQGPQGPVGAPGLKGDGYPGVPGPRGIPGPPGSRGLPGVGDTGAKGDPGVRGPPGPPGPRGIGTPGPKGDIGQKGLPGPPGSPGYGLQGIKGEQGPQGFPGPKGMTGHGLPGQKGEHGERGDVGKKGDKGEIGDPGPQGEQGLQGPKGDQGLTKEEIIKLIFEICGCGRKCKETPLELLFVIDSSESVGLKNFQIIKNFVKTLTDRVALDLATTRIGIINYSHKVEEVAHLTQFSSKDDLKQAVDDMQYLGEGTYTATALHAANRMFEAAKPGVKKVALVITDGQTDTRDKKNLTEVVKNASDINVEIFVIGVVKRNDPNFHIFHQEMNLIATDPDSEHVYLFDDFITLEDTLKQKLFTKSCEDFEAYLFHILGSSPLQPGFGISGEERHESTPEPQKEISESVHVSGAQDRENEPSERTWAASPATTPSPETATIPGPLPRPPEDQAKGLETRAPSSTSNLEPENFVHKDPRCLEPLQPGNCSEYVVRWYYDKQVNSCARFWFSGCKGSGNRFNSEKECEEVCIQG

Target ORF information:

RefSeq Version	XM_006074585.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type XXVIII, alpha 1 (COL28A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006074585.1

ORF Insert Sequence:

ATGTGGAACA GACATTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC GTTGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTCTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTATT ATGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCGTGAAAG TGACTGTGCT GATGATGGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGATGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ATGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAACGTAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAG GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGTGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGGCCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATTGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCTCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GGCCTCCAGG ACCTACTGGT 
GTAGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGACCAAAG GGTGAAAAAG GTTCTCAAGG ACCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATACGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TAGGACTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCAGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGCGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGGCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGC 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCCGGACC CCAAGGAGAA 
CAGGGGTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGGCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CGGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCATAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTGACTCAGT TCTCCAGCAA GGATGACCTC 
AAGCAGGCTG TGGACGACAT GCAGTATCTG GGGGAAGGTA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT CGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACCGATG GGCAGACAGA TACCCGAGAC AAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG CGAGCATGTT 
TATCTATTTG ATGACTTCAT TACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCATCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CCGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TACATGTCTC CGGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGACTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGGT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTATGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGTGA AAAGGAGTGT 
GAAGAAGTCT GCATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL28A1 ( XM_006074585.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006074647.1 Bubalus bubalis collagen type XXVIII alpha 1 chain (COL28A1), mRNA.

Price & Availability↑

CloneID	OBu70559
Clone ID Related Accession (Same CDS sequence)	XM_006074585.2
Accession Version	XM_006074585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037552.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006074585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACA GACATTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC GTTGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTCTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTATT ATGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCGTGAAAG TGACTGTGCT GATGATGGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGATGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ATGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAACGTAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAG GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGTGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGGCCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATTGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCTCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GGCCTCCAGG ACCTACTGGT 
GTAGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGACCAAAG GGTGAAAAAG GTTCTCAAGG ACCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATACGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TAGGACTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCAGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGCGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGGCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGT 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCTGGACC CCAAGGAGAA 
CAGGGGTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGGCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CGGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCATAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTGACTCAGT TCTCCAGCAA GGATGACCTC 
AAGCAGGCTG TGGACGACAT GCAGTATCTG GGGGAAGGTA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT CGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACCGATG GGCAGACAGA TACCCGAGAC AAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG CGAGCATGTT 
TATCTATTTG ATGACTTCAT TACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCATCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CCGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TACATGTCTC CGGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGACTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGGT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTACGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGTGA AAAGGAGTGT 
GAAGAAGTCT GCATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006074647.1
CDS	194..3637
Translation	MWNRHFVLYLLLLPAFMSQAIYGQRKKGSKTNLLARKNLQDSTCFIDLVFVVDSSESSKIFLFDKQKDFVGSLSDKIFQLTPVGSLKYDIKLAALQFSSSVQIDPPFSSWKDLQTFKQRVKSMNFIGQGTFSYYAIANATRLLKREGRKDGVKVTVLMMDGIDHPKNPDVQSISEDARTAGILFITIGLSAVVNETKLRLISGDLPSVPILLLNDSTLVDKIQDRLDILFEKKCERKICECEKGDPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGEPGERGPGGIPGYKGEKGERGECGKPGIKGDKGSPGPNGPKGPRGIQGIAGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIGQQGVKGQRGQEGRTGPPGPTGVGEPGQTGPRGPEGPPGERGLPGEGLPGPKGEKGSQGPIGPQGEAGPSIKGDKGDTGPVGPQGLMGIPGIGSQGEQGIQGPIGPPGPQGPPGQGSPGPKGEVGQMGPTGPRGPVGLGVQGPKGEPGSIGLPGQPGVPGEDGAAGKKGEAGLPGARGPEGPPGKGQPGLKGDEGKKGSKGNQGQKGSQGPGGPKGDPGIMGPVGMPGISIPGPPGPKGDRGGPGMPGFKGEPGIAIRGPKGAQGPQGPVGAPGLKGDGYPGVPGPRGIPGPPGSRGLPGVGDTGAKGDPGVRGPPGPPGPRGIGTPGPKGDIGQKGLPGPPGSPGYGLQGIKGEQGPQGFPGPKGMTGHGLPGQKGEHGERGDVGKKGDKGEIGDPGPQGEQGLQGPKGDQGLTKEEIIKLIFEICGCGRKCKETPLELLFVIDSSESVGLKNFQIIKNFVKTLTDRVALDLATTRIGIINYSHKVEEVAHLTQFSSKDDLKQAVDDMQYLGEGTYTATALHAANRMFEAAKPGVKKVALVITDGQTDTRDKKNLTEVVKNASDINVEIFVIGVVKRNDPNFHIFHQEMNLIATDPDSEHVYLFDDFITLEDTLKQKLFTKSCEDFEAYLFHILGSSPLQPGFGISGEERHESTPEPQKEISESVHVSGAQDRENEPSERTWAASPATTPSPETATIPGPLPRPPEDQAKGLETRAPSSTSNLEPENFVHKDPRCLEPLQPGNCSEYVVRWYYDKQVNSCARFWFSGCKGSGNRFNSEKECEEVCIQG

Target ORF information:

RefSeq Version	XM_006074585.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type XXVIII alpha 1 chain (COL28A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006074585.2

ORF Insert Sequence:

ATGTGGAACA GACATTTTGT CCTGTATCTC CTGCTTTTGC CAGCTTTTAT GAGTCAAGCA 
ATATATGGAC AAAGAAAGAA AGGCTCAAAG ACCAATTTAC TTGCAAGGAA AAATCTCCAG 
GACTCCACGT GCTTCATAGA TCTTGTCTTC GTTGTGGACA GCTCTGAAAG TTCCAAAATT 
TTTCTCTTTG ACAAACAAAA AGATTTTGTG GGTAGCTTGA GTGACAAGAT ATTTCAGTTG 
ACCCCAGTTG GCTCCTTGAA ATATGACATC AAACTGGCAG CCCTTCAGTT TAGCAGCTCT 
GTCCAAATTG ATCCACCTTT TTCCTCCTGG AAGGATCTAC AGACTTTTAA ACAGAGGGTC 
AAGTCTATGA ATTTCATTGG GCAAGGTACT TTCTCTTATT ATGCCATTGC CAATGCCACG 
AGGCTACTTA AGAGAGAAGG GCGTAAGGAT GGCGTGAAAG TGACTGTGCT GATGATGGAT 
GGCATTGACC ATCCAAAGAA TCCAGATGTT CAAAGTATTT CTGAAGATGC CAGAACTGCA 
GGAATATTAT TTATCACCAT TGGACTTTCT GCTGTAGTCA ATGAAACCAA ACTGCGTTTG 
ATATCTGGGG ATTTACCCAG TGTACCCATT CTCCTGTTGA ATGATTCAAC CCTTGTCGAC 
AAAATTCAGG ATCGCCTGGA CATCTTATTT GAAAAGAAGT GTGAACGTAA GATTTGTGAA 
TGTGAGAAGG GGGATCCCGG CGATCCAGGG CCTCCGGGTA CACATGGAAA CCCAGGTATC 
AAAGGTGAGC GAGGACCAAA AGGAAACCCG GGTGATGCTC AAAAAGGGGA ACCTGGAGAA 
AGAGGCCCAG GGGGAATCCC TGGGTACAAG GGTGAGAAGG GTGAACGTGG AGAATGTGGT 
AAACCCGGCA TAAAGGGTGA CAAAGGATCC CCAGGGCCAA ATGGACCAAA GGGACCCAGA 
GGAATTCAGG GCATTGCTGG ACCTCCTGGG GATCCAGGCC CGAAGGGATT TCAAGGCAAT 
AAGGGTGAAC CTGGCCCTCC TGGTCCCTAT GGTCCTCCAG GAGCCCCTGG TATTGGACAG 
CAAGGTGTTA AGGGGCAAAG AGGCCAGGAA GGAAGAACTG GGCCTCCAGG ACCTACTGGT 
GTAGGTGAAC CGGGCCAGAC AGGTCCCCGC GGGCCTGAGG GACCACCAGG AGAGAGAGGC 
TTACCAGGAG AAGGACTTCC AGGACCAAAG GGTGAAAAAG GTTCTCAAGG ACCAATTGGC 
CCACAAGGAG AAGCGGGCCC ATCAATAAAA GGGGACAAGG GGGATACGGG ACCTGTGGGA 
CCCCAAGGAC TGATGGGCAT CCCTGGAATT GGGAGTCAAG GGGAACAGGG AATCCAAGGT 
CCCATTGGCC CACCTGGTCC ACAAGGGCCC CCAGGACAAG GCTCACCTGG TCCCAAGGGA 
GAAGTTGGCC AGATGGGCCC TACAGGCCCT AGAGGACCAG TAGGACTCGG AGTACAAGGT 
CCAAAGGGGG AGCCAGGCTC AATAGGGCTC CCAGGACAGC CAGGAGTACC AGGAGAAGAT 
GGAGCTGCAG GGAAGAAGGG AGAAGCAGGG CTTCCGGGGG CAAGAGGCCC TGAAGGACCA 
CCTGGGAAAG GACAGCCTGG CCTCAAGGGA GATGAAGGAA AGAAAGGGAG CAAAGGAAAT 
CAAGGACAGA AAGGGTCTCA GGGGCCAGGA GGGCCAAAGG GTGATCCAGG AATTATGGGC 
CCTGTTGGGA TGCCTGGAAT ATCAATTCCT GGACCACCTG GACCAAAAGG AGACAGAGGT 
GGACCTGGGA TGCCTGGATT TAAAGGAGAA CCTGGAATTG CTATTCGAGG ACCAAAGGGT 
GCCCAAGGCC CGCAGGGACC AGTGGGCGCT CCAGGACTCA AAGGTGACGG CTATCCTGGT 
GTGCCCGGCC CTCGAGGAAT CCCAGGACCT CCTGGATCAA GGGGATTACC TGGAGTTGGA 
GACACTGGAG CAAAGGGAGA TCCTGGCGTC AGAGGCCCTC CAGGGCCCCC TGGGCCTCGG 
GGCATAGGAA CCCCAGGGCC AAAGGGTGAC ATCGGGCAGA AAGGCTTGCC TGGCCCTCCT 
GGCTCCCCTG GCTATGGATT ACAAGGAATC AAAGGGGAGC AAGGACCTCA GGGCTTCCCA 
GGGCCAAAGG GCATGACAGG CCATGGCCTC CCTGGCCAGA AGGGAGAGCA CGGAGAACGT 
GGCGATGTGG GAAAGAAAGG TGATAAAGGG GAAATTGGAG ATCCTGGACC CCAAGGAGAA 
CAGGGGTTAC AAGGACCAAA AGGAGACCAA GGACTTACAA AAGAAGAAAT CATCAAGCTT 
ATTTTTGAAA TATGTGGTTG TGGGCGTAAA TGCAAAGAGA CTCCGCTGGA GCTGCTGTTT 
GTGATTGACA GCTCAGAAAG CGTGGGGCTC AAGAACTTCC AAATCATCAA GAATTTTGTG 
AAGACCCTGA CGGACCGTGT CGCCCTGGAC CTGGCCACAA CCCGCATAGG CATAATCAAC 
TACAGCCATA AGGTGGAGGA GGTGGCGCAC CTGACTCAGT TCTCCAGCAA GGATGACCTC 
AAGCAGGCTG TGGACGACAT GCAGTATCTG GGGGAAGGTA CCTACACGGC CACAGCGCTC 
CATGCCGCCA ACCGTATGTT CGAAGCTGCC AAGCCCGGCG TGAAGAAGGT GGCCTTGGTC 
ATCACCGATG GGCAGACAGA TACCCGAGAC AAGAAGAATC TGACTGAGGT GGTGAAGAAC 
GCCAGTGACA TCAATGTGGA AATATTTGTG ATTGGGGTAG TGAAGAGGAA TGACCCCAAC 
TTCCACATTT TCCACCAAGA AATGAATCTG ATTGCTACTG ACCCAGATAG CGAGCATGTT 
TATCTATTTG ATGACTTCAT TACTCTTGAA GATACCCTGA AGCAAAAATT ATTTACAAAA 
AGTTGTGAGG ATTTCGAAGC CTACCTCTTT CATATTTTGG GTTCATCACC GCTTCAACCT 
GGATTTGGGA TATCGGGGGA AGAACGCCAT GAATCCACTC CCGAGCCCCA GAAAGAAATT 
TCTGAGTCTG TACATGTCTC CGGGGCCCAG GACAGAGAGA ATGAGCCTTC AGAGAGGACC 
TGGGCTGCCA GCCCGGCCAC CACTCCTTCA CCTGAGACTG CCACCATCCC GGGGCCACTG 
CCCAGGCCTC CTGAGGATCA GGCAAAGGGC CTGGAAACCA GAGCTCCGAG TTCCACTTCG 
AACTTAGAGC CAGAAAATTT TGTGCACAAA GATCCTAGGT GTCTGGAACC CCTACAACCA 
GGGAACTGTA GTGAATATGT GGTTCGATGG TATTACGACA AACAGGTCAA TTCCTGCGCT 
CGCTTTTGGT TCAGTGGCTG TAAAGGCTCG GGAAATAGAT TCAACAGTGA AAAGGAGTGT 
GAAGAAGTCT GCATTCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL28A1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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