RAPGEF3 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF3
Entrez Gene ID	102407658
Full Name	Rap guanine nucleotide exchange factor 3
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006059454.1	XP_006059516.1	rap guanine nucleotide exchange factor 3 isoform X2
XM_006059454.2	XP_006059516.1	rap guanine nucleotide exchange factor 3 isoform X2
XM_025284046.1	XP_025139831.1	rap guanine nucleotide exchange factor 3 isoform X3
XM_006059453.1	XP_006059515.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_006059453.2	XP_006059515.1	rap guanine nucleotide exchange factor 3 isoform X1

Pathways from BioSystems
Gene Ontology

The following RAPGEF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu16047	XM_006059453.1 Latest version!	Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu16048	XM_006059454.1 Latest version!	Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu64786	XM_006059453.2 Latest version!	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu64787	XM_006059454.2 Latest version!	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu64788	XM_025284046.1 Latest version!	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF3 ( XM_006059453.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059515.1 Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu16047
Clone ID Related Accession (Same CDS sequence)	XM_006059453.1
Accession Version	XM_006059453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGACAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCAGCCCT TGAGAGGCTG TTGGACCCCT CGTGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACGTGACCT TCATCCACGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCTCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059515.1
CDS	535..3306
Translation	MKVGWPGESRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASTERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALYHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYVCNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDARLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVQELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDVTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_006059453.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059453.1

ORF Insert Sequence:

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGACAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCAGCCCT TGAGAGGCTG TTGGACCCCT CGTGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACGTGACCT TCATCCACGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCTCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_006059454.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059516.1 Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu16048
Clone ID Related Accession (Same CDS sequence)	XM_006059454.1
Accession Version	XM_006059454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGACAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGGACCCCTC GTGGAACCAC 
CGAGTGTACC GTCTGGCCCT CACCAAGCTC TCCCCTCCCC TCATCCCCTT CATGCCCCTT 
CTTCTCAAAG ACGTGACCTT CATCCACGAG GGAAATCACA CATTGGTGGA GAATCTCATC 
AACTTTGAGA AGATGAGAAT GATGGCCAGA GCTGCACGGA TGCTACACCA CTGCCGAAGC 
CACAGCAATG TGCCTCTGTC ACCGCTCAGA AGCCGAGTCT CCCACCTGCA CGAGGACAGC 
CAGGCGGTCA GGGTTTCCAC GTGCTCGGAG CAGTCCCTGA GCACCCGGAG TCCCGCCAGC 
ACCTGGGCTT ACGTCCAGCA GCTGAAGGTC ATCGACAACC AGCGGGAACT GTCCCGCCTC 
TCTCGGGAGC TGGAGCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059516.1
CDS	538..3258
Translation	MKVGWPGESRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASTERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALYHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYVCNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDARLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVQELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWEDPSWNHRVYRLALTKLSPPLIPFMPLLLKDVTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_006059454.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059454.1

ORF Insert Sequence:

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGACAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGGACCCCTC GTGGAACCAC 
CGAGTGTACC GTCTGGCCCT CACCAAGCTC TCCCCTCCCC TCATCCCCTT CATGCCCCTT 
CTTCTCAAAG ACGTGACCTT CATCCACGAG GGAAATCACA CATTGGTGGA GAATCTCATC 
AACTTTGAGA AGATGAGAAT GATGGCCAGA GCTGCACGGA TGCTACACCA CTGCCGAAGC 
CACAGCAATG TGCCTCTGTC ACCGCTCAGA AGCCGAGTCT CCCACCTGCA CGAGGACAGC 
CAGGCGGTCA GGGTTTCCAC GTGCTCGGAG CAGTCCCTGA GCACCCGGAG TCCCGCCAGC 
ACCTGGGCTT ACGTCCAGCA GCTGAAGGTC ATCGACAACC AGCGGGAACT GTCCCGCCTC 
TCTCGGGAGC TGGAGCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_006059453.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059515.1 Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu64786
Clone ID Related Accession (Same CDS sequence)	XM_006059453.2
Accession Version	XM_006059453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006059453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCAGCCCT TGAGAGGCTG TTGGACCCCT CGTGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACGTGACCT TCATCCACGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCTCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059515.1
CDS	528..3299
Translation	MKVGWPGESRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASTERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALYHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYVCNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDARLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVQELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDVTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_006059453.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059453.2

ORF Insert Sequence:

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCAGCCCT TGAGAGGCTG TTGGACCCCT CGTGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACGTGACCT TCATCCACGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCTCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_006059454.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059516.1 Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu64787
Clone ID Related Accession (Same CDS sequence)	XM_006059454.2
Accession Version	XM_006059454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006059454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGGACCCCTC GTGGAACCAC 
CGAGTGTACC GTCTGGCCCT CACCAAGCTC TCCCCTCCCC TCATCCCCTT CATGCCCCTT 
CTTCTCAAAG ACGTGACCTT CATCCACGAG GGAAATCACA CATTGGTGGA GAATCTCATC 
AACTTTGAGA AGATGAGAAT GATGGCCAGA GCTGCACGGA TGCTACACCA CTGCCGAAGC 
CACAGCAATG TGCCTCTGTC ACCGCTCAGA AGCCGAGTCT CCCACCTGCA CGAGGACAGC 
CAGGCGGTCA GGGTTTCCAC GTGCTCGGAG CAGTCCCTGA GCACCCGGAG TCCCGCCAGC 
ACCTGGGCTT ACGTCCAGCA GCTGAAGGTC ATCGACAACC AGCGGGAACT GTCCCGCCTC 
TCTCGGGAGC TGGAGCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059516.1
CDS	534..3254
Translation	MKVGWPGESRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASTERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALYHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYVCNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDARLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVQELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWEDPSWNHRVYRLALTKLSPPLIPFMPLLLKDVTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_006059454.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059454.2

ORF Insert Sequence:

ATGAAGGTGG GCTGGCCAGG TGAGAGCCGC TGGCAGGTGG GCCTGGCTGT GGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCA CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGTGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTACCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGCG TCCATGAGCT GGAGGAGGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCG 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAC GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCATGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG TAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCTGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCTCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCATG CGGAGCCCGC TGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CGTCTGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CGCTGTATGG CCCTACGCTC 
CGCACTGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
GCCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCTCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC GTGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCC CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCAGGAG 
CTGACTCCAC ACCCCGATCA GCTGGGTCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGATCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCCGT GCCTGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCGT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGGACCCCTC GTGGAACCAC 
CGAGTGTACC GTCTGGCCCT CACCAAGCTC TCCCCTCCCC TCATCCCCTT CATGCCCCTT 
CTTCTCAAAG ACGTGACCTT CATCCACGAG GGAAATCACA CATTGGTGGA GAATCTCATC 
AACTTTGAGA AGATGAGAAT GATGGCCAGA GCTGCACGGA TGCTACACCA CTGCCGAAGC 
CACAGCAATG TGCCTCTGTC ACCGCTCAGA AGCCGAGTCT CCCACCTGCA CGAGGACAGC 
CAGGCGGTCA GGGTTTCCAC GTGCTCGGAG CAGTCCCTGA GCACCCGGAG TCCCGCCAGC 
ACCTGGGCTT ACGTCCAGCA GCTGAAGGTC ATCGACAACC AGCGGGAACT GTCCCGCCTC 
TCTCGGGAGC TGGAGCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_025284046.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139831.1 Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

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CloneID	OBu64788
Clone ID Related Accession (Same CDS sequence)	XM_025284046.1
Accession Version	XM_025284046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rap guanine nucleotide exchange factor 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCACGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGTG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT ACCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGCGTCCATG AGCTGGAGGA GGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCGCTTCGA 
AAGCCCCCTG GTCAGCGCAC AGACGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCA 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGTAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCT 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCT CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CATGCGGAGC CCGCTGGGGG CAGCGAGCAG GAGCGCAGCT CCTACGTCTG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACGCTGT ATGGCCCTAC GCTCCGCACT 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACGCCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGCGTCTCC TCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCGTGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCCCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA GGAGCTGACT 
CCACACCCCG ATCAGCTGGG TCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAT CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CCGTGCCTGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCGTCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCAG CCCTTGAGAG GCTGTTGGAC CCCTCGTGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACGTG 
ACCTTCATCC ACGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCTCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139831.1
CDS	243..2888
Translation	MVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASTERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALYHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYVCNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDARLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVQELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDVTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_025284046.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025284046.1

ORF Insert Sequence:

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCACGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGTG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT ACCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGCGTCCATG AGCTGGAGGA GGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCGCTTCGA 
AAGCCCCCTG GTCAGCGCAC AGACGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCA 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGTAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCT 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCT CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CATGCGGAGC CCGCTGGGGG CAGCGAGCAG GAGCGCAGCT CCTACGTCTG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACGCTGT ATGGCCCTAC GCTCCGCACT 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACGCCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGCGTCTCC TCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCGTGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCCCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA GGAGCTGACT 
CCACACCCCG ATCAGCTGGG TCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAT CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CCGTGCCTGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCGTCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCAG CCCTTGAGAG GCTGTTGGAC CCCTCGTGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACGTG 
ACCTTCATCC ACGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCTCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RAPGEF3 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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