CACNA1F cDNA ORF clone, Bubalus bubalis(water buffalo)

The following CACNA1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu73378 XM_025275895.1
Latest version!
Bubalus bubalis calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu27683 XM_006073652.1
Latest version!
Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBu27684 XM_006073653.1
Latest version!
Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBu27685 XM_006073654.1
Latest version!
Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu73378
    Clone ID Related Accession (Same CDS sequence) XM_025275895.1
    Accession Version XM_025275895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product voltage-dependent L-type calcium channel subunit alpha-1F
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG
    GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTGCGCTGGT TCAGTCACTC CACCCACTCC
    ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA
    GACGAGGAAG AGGAAGAGGG CACTCTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCATG
    AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG
    AGGGCCGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT
    CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT
    GCCAACAAAG TGCTACTCTG TCTGTTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG
    GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG
    GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA
    CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC
    AATTTAGTGG CGTCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC
    TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC
    TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT
    GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC
    TATGGCGGCC CCTTCTTCCC AGGGATGCTG GTGTGCGTGT ATTTCATCAT CCTCTTCATC
    TGTGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT
    GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG
    GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA
    GGAGCAGACA TGGAGGAAGA GGAGGAGGAG GAGGAAGAGG AGGAAGAGGA AGTGGGGGCC
    GGGCATGTGG AACTCCTGCA GGAAGTTGTA CCCAAGGAGA AGGTGGTGCC CATTCCTGAG
    GGCAGTGCTT TCTTCTGCCT CAGCCAAACC AACCCGCTGA GGAAGGCCTG CCACACCCTC
    ATCCACCATC ACATCTTCAC CAATCTCATC CTGGTGTTTA TCATCCTCAG CAGTGTGTCC
    CTGGCCGCTG AGGATCCCAT CCGAGCCCAC TCCTTCCGCA ACCACATTCT GGGGTACTTC
    GATTATGCCT TCACCTCCAT TTTCACTGTG GAGATTCTAC TAAAGATGAC AGTGTTCGGG
    GCTTTTCTGC ACCGTGGTTC CTTCTGCCGT AGCTGGTTCA ATCTGCTGGA TCTGCTGGTG
    GTCAGCGTGT CCCTCATCTC CTTTGGCATC CACTCCAGCG CCATCTCAGT GGTGAAGATT
    CTGCGTGTAC TCCGAGTACT GCGGCCCCTT CGAGCTATCA ACAGGGCCAA AGGACTCAAG
    CACGTGGTGC AATGTGTGTT CGTGGCCATC CGGACCATCG GAAATATCAT GATCGTGACC
    ACGCTCCTGC AGTTCATGTT CGCCTGCATT GGTGTGCAGC TCTTCAAGGG GAAATTCTAC
    AGTTGCACTG ATGAGGCCAA ACACACACCC CAAGAATGCA AGGGCTCCTT CCTGGTCTAC
    CCCGATGGAG ATGTGTCAAG GCCTCTGGTC CGGGAGCGGC TCTGGGTCAA CAGCGATTTC
    AACTTTGACA ATGTCCTTTC AGCCATGATG GCCCTGTTCA CTGTCTCCAC CTTCGAAGGC
    TGGCCTGCGC TGCTATACAA GGCCATTGAT GCAAACGCAG AGGACAAGGG CCCCATCTAC
    AATTACCACG TGGAGATCTC AGTGTTCTTC ATTGTCTACA TCATCATCAT TGCATTCTTC
    ATGATGAACA TCTTTGTGGG CTTTGTCATC ATCACCTTCC GAGCCCAGGG CGAGCAGGAG
    TACCAAGACT GTGAGCTGGA TAAGAACCAG CGCCAGTGTG TAGAATATGC CCTCAAGGCC
    CAACCACTCC GTCGCTATAT CCCCAAGAAC CCACATCAGT ATCGTGTGTG GGCCACTGTG
    AACTCTGCTG CCTTCGAGTA TCTCATGTTC CTGCTCATCC TGCTCAACAC GGTTGCTCTA
    GCCATGCAGC ACTATGAGCA GACTGCTCCC TTCAACTATG CCATGGACAT CCTCAACATG
    GTCTTCACTG GCCTCTTCAC TGTTGAGATG GTGCTCAAAA TCATTGCCTT CAAACCCAAG
    CATTACTTTA CTGATGCCTG GAACACATTT GATGCTCTTA TTGTGGTGGG CAGCGTAGTG
    GACATTGCCG TCACTGAAGT CAATAGCTCT GAGGATAGTT CCCGCATTTC GATCACCTTC
    TTTCGCCTCT TCCGAGTCAT GCGATTGGTC AAGCTTCTTA GTAAGGGTGA AGGGATTCGC
    ACATTACTTT GGACATTTAT CAAGTCTTTT CAGGCCTTAC CCTATGTGGC TCTTCTCATC
    GCAATGATAT TCTTCATTTA CGCAGTCATT GGGATGCAGA TGTTTGGCAA GGTGGCTCTT
    CAGGATGGCA CACAGATCAA CCGAAACAAC AACTTCCAGA CCTTTCCACA GGCTGTACTG
    CTTTTGTTCA GGTGTGCTAC TGGTGAGGCA TGGCAGGAGA TAATGCTTGC CAGCCTTCCT
    GGAAGCCGGT GTGACCCTGA GTCTGACTTC GGCCCTGGTG AGGAGTTTAG CTGTGGTAGC
    AATTTTGCCA TCGCCTATTT TATCAGCTTC TTCATGCTCT GTGCATTCCT GATCATAAAT
    CTCTTTGTGG CTGTGATCAT GGACAACTTT GATTATCTAA CCAGAGATTG GTCCATCCTG
    GGCCCCCATC ACCTCGATGA ATTCAAGAGG ATTTGGTCTG AATATGACCC TGGGGCCAAA
    GGCCGCATCA AGCACCTGGA TGTGGTTGCC CTGCTGAGAC GCATTCAGCC CCCCCTGGGA
    TTCGGGAAGC TGTGCCCGCA CCGAGTGGCC TGCAAGAGAC TTGTGGCGAT GAACATGCCC
    CTCAACTCAG ATGGGACAGT GACATTCAAT GCCACACTCT TTGCCCTGGT CCGGACATCA
    CTGAAGATCA AGACGGAAGG AAACCTGGAG CAAGCCAATC AGGAGCTGCG GATTGTCATC
    AAAAAGATCT GGAAGCGGAT GAAGCAGAAG CTGCTAGATG AGGTCATCCC ACCAGCTGAT
    GAGGAAGAAG TTACCGTGGG CAAATTCTAC GCCACATTTC TGATCCAGGA CTACTTCCGC
    AAATTCCGGC GGAGGAAAGA AAAGGGGCTA CTAGGCACCG AGGCCCCCCC AAGCACCTCC
    TCTGCCCTTC AGGCTGGCCT GAGGAGCCTG CAAGACTTGG GTCCGGAGAT GCGGCAGGCC
    CTCACTTGTG ACACAGAGGA AGAGGAAGGG GAAGAGGGGC AGGAGGGAGA GGAGGAACAC
    GAGGACCTGG AAACGTACAC AGCCCTGATG GGCTCCCAGC CCCCATCTCG CCGGAGCTCC
    GTGATTTCTG TGTCTCTACC TGCTGGAGAC AAACCTCCAG ATTCACTTTC CCCTGGACCC
    AGTGATGACG ATGGGGGGGC TCCCAACTCC AGACAGTCCA ATGTTCCCCA GTCTGGGTCC
    CATGCCCACA GGAGAAGCTC TGGGGTTCTC ATTTTCACCA TTCCAGAAGA AAGAAGTTCT
    CAGTCCAAGG CAACCAAAGG GCAAGAGAAG CAGGATGAGC AAGAGGAAGT CCTGAGCCAG
    CACTCTGGCC ACGACACGCT CTCCTACCTA GATGAACAGG CAGGGGCTCC CCCACGCCCC
    ATCCTTTTGC CATCTCACAG ACCCCAGAGA TACGTGGATG GCCGCCAAGC ACCACGCCGC
    CGTCTGCTGC CCCCAACGCC TGCAGGTCGG AAGCCATCCT TCACCATCCA GTGTCTGCGG
    CGCCAGGGCA GCTGTGAAGA TTTACCCATC CCAGGCACCT ATCATCGTGG ACGAAACTCA
    GGGCCCAGTA GGGCACAGGG TTCCTGGGCA ACCCCTCCTC AGCGGGGCCG ACTCCTATAT
    GCCCCACTGC TATTGGTGGA GGAGGGTGCA GCAGGGGAGG GATACATCGG CAAATCCAGC
    AGTCCACTGC GCACCTTCAC CTGTCTGCAT GTGCCTGGAA CCCACTTGGA CCCCAGCCAT
    GGCAAAAGGG GCAGTGCTGA CAGCCTGGTG GAGGCTGTGC TCATCTCTGA GGGCCTGGGC
    CTCTTTGCCC GAGACCCACG CTTCGTGGCC CTGGCCAAGC AGGAGATTGC AGATGCATGT
    CGCCTGACCC TGGACGAGAT GGACAGTGCT GCCAGTGACC TGCTGGCACA GGGGACCAGC
    TCACTGTATA GTGACGAAGA GTCCATCCTC TCCCGTTTTG ATGAGGAGGA CCTAGGAGAC
    GAGATGGCCT GCGTCCATGC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025131680.1
    CDS1..5907
    Translation

    Target ORF information:

    RefSeq Version XM_025275895.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025275895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG
    GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTGCGCTGGT TCAGTCACTC CACCCACTCC
    ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA
    GACGAGGAAG AGGAAGAGGG CACTCTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCATG
    AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG
    AGGGCCGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT
    CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT
    GCCAACAAAG TGCTACTCTG TCTGTTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG
    GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG
    GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA
    CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC
    AATTTAGTGG CGTCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC
    TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC
    TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT
    GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC
    TATGGCGGCC CCTTCTTCCC AGGGATGCTG GTGTGCGTGT ATTTCATCAT CCTCTTCATC
    TGTGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT
    GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG
    GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA
    GGAGCAGACA TGGAGGAAGA GGAGGAGGAG GAGGAAGAGG AGGAAGAGGA AGTGGGGGCC
    GGGCATGTGG AACTCCTGCA GGAAGTTGTA CCCAAGGAGA AGGTGGTGCC CATTCCTGAG
    GGCAGTGCTT TCTTCTGCCT CAGCCAAACC AACCCGCTGA GGAAGGCCTG CCACACCCTC
    ATCCACCATC ACATCTTCAC CAATCTCATC CTGGTGTTTA TCATCCTCAG CAGTGTGTCC
    CTGGCCGCTG AGGATCCCAT CCGAGCCCAC TCCTTCCGCA ACCACATTCT GGGGTACTTC
    GATTATGCCT TCACCTCCAT TTTCACTGTG GAGATTCTAC TAAAGATGAC AGTGTTCGGG
    GCTTTTCTGC ACCGTGGTTC CTTCTGCCGT AGCTGGTTCA ATCTGCTGGA TCTGCTGGTG
    GTCAGCGTGT CCCTCATCTC CTTTGGCATC CACTCCAGCG CCATCTCAGT GGTGAAGATT
    CTGCGTGTAC TCCGAGTACT GCGGCCCCTT CGAGCTATCA ACAGGGCCAA AGGACTCAAG
    CACGTGGTGC AATGTGTGTT CGTGGCCATC CGGACCATCG GAAATATCAT GATCGTGACC
    ACGCTCCTGC AGTTCATGTT CGCCTGCATT GGTGTGCAGC TCTTCAAGGG GAAATTCTAC
    AGTTGCACTG ATGAGGCCAA ACACACACCC CAAGAATGCA AGGGCTCCTT CCTGGTCTAC
    CCCGATGGAG ATGTGTCAAG GCCTCTGGTC CGGGAGCGGC TCTGGGTCAA CAGCGATTTC
    AACTTTGACA ATGTCCTTTC AGCCATGATG GCCCTGTTCA CTGTCTCCAC CTTCGAAGGC
    TGGCCTGCGC TGCTATACAA GGCCATTGAT GCAAACGCAG AGGACAAGGG CCCCATCTAC
    AATTACCACG TGGAGATCTC AGTGTTCTTC ATTGTCTACA TCATCATCAT TGCATTCTTC
    ATGATGAACA TCTTTGTGGG CTTTGTCATC ATCACCTTCC GAGCCCAGGG CGAGCAGGAG
    TACCAAGACT GTGAGCTGGA TAAGAACCAG CGCCAGTGTG TAGAATATGC CCTCAAGGCC
    CAACCACTCC GTCGCTATAT CCCCAAGAAC CCACATCAGT ATCGTGTGTG GGCCACTGTG
    AACTCTGCTG CCTTCGAGTA TCTCATGTTC CTGCTCATCC TGCTCAACAC GGTTGCTCTA
    GCCATGCAGC ACTATGAGCA GACTGCTCCC TTCAACTATG CCATGGACAT CCTCAACATG
    GTCTTCACTG GCCTCTTCAC TGTTGAGATG GTGCTCAAAA TCATTGCCTT CAAACCCAAG
    CATTACTTTA CTGATGCCTG GAACACATTT GATGCTCTTA TTGTGGTGGG CAGCGTAGTG
    GACATTGCCG TCACTGAAGT CAATAGCTCT GAGGATAGTT CCCGCATTTC GATCACCTTC
    TTTCGCCTCT TCCGAGTCAT GCGATTGGTC AAGCTTCTTA GTAAGGGTGA AGGGATTCGC
    ACATTACTTT GGACATTTAT CAAGTCTTTT CAGGCCTTAC CCTATGTGGC TCTTCTCATC
    GCAATGATAT TCTTCATTTA CGCAGTCATT GGGATGCAGA TGTTTGGCAA GGTGGCTCTT
    CAGGATGGCA CACAGATCAA CCGAAACAAC AACTTCCAGA CCTTTCCACA GGCTGTACTG
    CTTTTGTTCA GGTGTGCTAC TGGTGAGGCA TGGCAGGAGA TAATGCTTGC CAGCCTTCCT
    GGAAGCCGGT GTGACCCTGA GTCTGACTTC GGCCCTGGTG AGGAGTTTAG CTGTGGTAGC
    AATTTTGCCA TCGCCTATTT TATCAGCTTC TTCATGCTCT GTGCATTCCT GATCATAAAT
    CTCTTTGTGG CTGTGATCAT GGACAACTTT GATTATCTAA CCAGAGATTG GTCCATCCTG
    GGCCCCCATC ACCTCGATGA ATTCAAGAGG ATTTGGTCTG AATATGACCC TGGGGCCAAA
    GGCCGCATCA AGCACCTGGA TGTGGTTGCC CTGCTGAGAC GCATTCAGCC CCCCCTGGGA
    TTCGGGAAGC TGTGCCCGCA CCGAGTGGCC TGCAAGAGAC TTGTGGCGAT GAACATGCCC
    CTCAACTCAG ATGGGACAGT GACATTCAAT GCCACACTCT TTGCCCTGGT CCGGACATCA
    CTGAAGATCA AGACGGAAGG AAACCTGGAG CAAGCCAATC AGGAGCTGCG GATTGTCATC
    AAAAAGATCT GGAAGCGGAT GAAGCAGAAG CTGCTAGATG AGGTCATCCC ACCAGCTGAT
    GAGGAAGAAG TTACCGTGGG CAAATTCTAC GCCACATTTC TGATCCAGGA CTACTTCCGC
    AAATTCCGGC GGAGGAAAGA AAAGGGGCTA CTAGGCACCG AGGCCCCCCC AAGCACCTCC
    TCTGCCCTTC AGGCTGGCCT GAGGAGCCTG CAAGACTTGG GTCCGGAGAT GCGGCAGGCC
    CTCACTTGTG ACACAGAGGA AGAGGAAGGG GAAGAGGGGC AGGAGGGAGA GGAGGAACAC
    GAGGACCTGG AAACGTACAC AGCCCTGATG GGCTCCCAGC CCCCATCTCG CCGGAGCTCC
    GTGATTTCTG TGTCTCTACC TGCTGGAGAC AAACCTCCAG ATTCACTTTC CCCTGGACCC
    AGTGATGACG ATGGGGGGGC TCCCAACTCC AGACAGTCCA ATGTTCCCCA GTCTGGGTCC
    CATGCCCACA GGAGAAGCTC TGGGGTTCTC ATTTTCACCA TTCCAGAAGA AAGAAGTTCT
    CAGTCCAAGG CAACCAAAGG GCAAGAGAAG CAGGATGAGC AAGAGGAAGT CCTGAGCCAG
    CACTCTGGCC ACGACACGCT CTCCTACCTA GATGAACAGG CAGGGGCTCC CCCACGCCCC
    ATCCTTTTGC CATCTCACAG ACCCCAGAGA TACGTGGATG GCCGCCAAGC ACCACGCCGC
    CGTCTGCTGC CCCCAACGCC TGCAGGTCGG AAGCCATCCT TCACCATCCA GTGTCTGCGG
    CGCCAGGGCA GCTGTGAAGA TTTACCCATC CCAGGCACCT ATCATCGTGG ACGAAACTCA
    GGGCCCAGTA GGGCACAGGG TTCCTGGGCA ACCCCTCCTC AGCGGGGCCG ACTCCTATAT
    GCCCCACTGC TATTGGTGGA GGAGGGTGCA GCAGGGGAGG GATACATCGG CAAATCCAGC
    AGTCCACTGC GCACCTTCAC CTGTCTGCAT GTGCCTGGAA CCCACTTGGA CCCCAGCCAT
    GGCAAAAGGG GCAGTGCTGA CAGCCTGGTG GAGGCTGTGC TCATCTCTGA GGGCCTGGGC
    CTCTTTGCCC GAGACCCACG CTTCGTGGCC CTGGCCAAGC AGGAGATTGC AGATGCATGT
    CGCCTGACCC TGGACGAGAT GGACAGTGCT GCCAGTGACC TGCTGGCACA GGGGACCAGC
    TCACTGTATA GTGACGAAGA GTCCATCCTC TCCCGTTTTG ATGAGGAGGA CCTAGGAGAC
    GAGATGGCCT GCGTCCATGC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu27683
    Clone ID Related Accession (Same CDS sequence) XM_006073652.1
    Accession Version XM_006073652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG
    GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTGCGCTGGT TCAGTCACTC CACCCACTCC
    ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA
    GACGAGGAAG AGGAAGAGGG CACTCTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCATG
    AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG
    AGGGCCGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT
    CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT
    GCCAACAAAG TGCTACTCTG TCTGTTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG
    GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG
    GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA
    CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC
    AATTTAGTGG CATCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC
    TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC
    TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT
    GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC
    TATGGCGGCC CCTTCTTCCC AGGGATGCTG GTGTGCGTGT ATTTCATCAT CCTCTTCATC
    TGTGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT
    GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG
    GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA
    GGAGCAGACA TGGAGGAAGA GGAGGAGGAG GAGGAAGAGG AGGAAGAGGA AGTGGGGGCC
    GGGCATGTGG AACTCCTGCA GGAAGTTGTA CCCAAGGAGA AGGTGGTGCC CATTCCTGAG
    GGCAGTGCTT TCTTCTGCCT CAGCCAAACC AACCCGCTGA GGAAGGCCTG CCACACCCTC
    ATCCACCATC ACATCTTCAC CAATCTCATC CTGGTGTTTA TCATCCTCAG CAGTGTGTCC
    CTGGCCGCTG AGGATCCCAT CCGAGCCCAC TCCTTCCGCA ACCACATTCT GGGGTACTTC
    GATTATGCCT TCACCTCCAT TTTCACTGTG GAGATTCTAC TAAAGATGAC AGTGTTCGGG
    GCTTTTCTGC ACCGTGGTTC CTTCTGCCGT AGCTGGTTCA ATCTGCTGGA TCTGCTGGTG
    GTCAGCGTGT CCCTCATCTC CTTTGGCATC CACTCCAGCG CCATCTCAGT GGTGAAGATT
    CTGCGTGTAC TCCGAGTACT GCGGCCCCTT CGAGCTATCA ACAGGGCCAA AGGACTCAAG
    CACGTGGTGC AATGTGTGTT CGTGGCCATC CGGACCATCG GAAATATCAT GATCGTGACC
    ACGCTCCTGC AGTTCATGTT CGCCTGCATT GGTGTGCAGC TCTTCAAGGG GAAATTCTAC
    AGTTGCACTG ATGAGGCCAA ACACACACCC CAAGAATGCA AGGGCTCCTT CCTGGTCTAC
    CCCGATGGAG ATGTGTCAAG GCCTCTGGTC CGGGAGCGGC TCTGGGTCAA CAGCGATTTC
    AACTTTGACA ATGTCCTTTC AGCCATGATG GCCCTGTTCA CTGTCTCCAC CTTCGAAGGC
    TGGCCTGCGC TGCTATACAA GGCCATTGAT GCAAACGCAG AGGACAAGGG CCCCATCTAC
    AATTACCACG TGGAGATCTC AGTGTTCTTC ATTGTCTACA TCATCATCAT TGCATTCTTC
    ATGATGAACA TCTTTGTGGG CTTTGTCATC ATCACCTTCC GAGCCCAGGG CGAGCAGGAG
    TACCAAGACT GTGAGCTGGA TAAGAACCAG CGCCAGTGTG TAGAATACGC CCTCAAGGCC
    CAACCACTCC GTCGCTATAT CCCCAAGAAC CCACATCAGT ATCGTGTGTG GGCCACTGTG
    AACTCTGCTG CCTTCGAGTA TCTCATGTTC CTGCTCATCC TGCTCAACAC GGTTGCTCTA
    GCCATGCAGC ACTATGAGCA GACTGCTCCC TTCAACTATG CCATGGACAT CCTCAACATG
    GTCTTCACTG GCCTCTTCAC TGTTGAGATG GTGCTCAAAA TCATTGCCTT CAAACCCAAG
    CATTACTTTA CTGATGCCTG GAACACATTT GATGCTCTTA TTGTGGTGGG CAGCGTAGTG
    GACATTGCCG TCACTGAAGT CAATAATGGT GGCCACCTGG GCGAGAGCTC TGAGGATAGT
    TCCCGCATTT CGATCACCTT CTTTCGCCTC TTCCGAGTCA TGCGATTGGT CAAGCTTCTT
    AGTAAGGGTG AAGGGATTCG CACATTACTT TGGACATTTA TCAAGTCTTT TCAGGCCTTA
    CCCTATGTGG CTCTTCTCAT CGCAATGATA TTCTTCATTT ACGCAGTCAT TGGGATGCAG
    ATGTTTGGCA AGGTGGCTCT TCAGGATGGC ACACAGATCA ACCGAAACAA CAACTTCCAG
    ACCTTTCCAC AGGCTGTACT GCTTTTGTTC AGGTGTGCTA CTGGTGAGGC ATGGCAGGAG
    ATAATGCTTG CCAGCCTTCC TGGAAGCCGG TGTGACCCTG AGTCTGACTT CGGCCCTGGT
    GAGGAGTTTA GCTGTGGTAG CAATTTTGCC ATCGCCTATT TTATCAGCTT CTTCATGCTC
    TGTGCATTCC TGATCATAAA TCTCTTTGTG GCTGTGATCA TGGACAACTT TGATTATCTA
    ACCAGAGATT GGTCCATCCT GGGCCCCCAT CACCTCGATG AATTCAAGAG GATTTGGTCT
    GAATATGACC CTGGGGCCAA AGGCCGCATC AAGCACCTGG ATGTGGTTGC CCTGCTGAGA
    CGCATTCAGC CCCCCCTGGG ATTCGGGAAG CTGTGCCCGC ACCGAGTGGC CTGCAAGAGA
    CTTGTGGCGA TGAACATGCC CCTCAACTCA GATGGGACAG TGACATTCAA TGCCACACTC
    TTTGCCCTGG TCCGGACATC ACTGAAGATC AAGACGGAAG GAAACCTGGA GCAAGCCAAT
    CAGGAGCTGC GGATTGTCAT CAAAAAGATC TGGAAGCGGA TGAAGCAGAA GCTGCTAGAT
    GAGGTCATCC CACCAGCTGA TGAGGAAGAA GTTACCGTGG GCAAATTCTA CGCCACATTT
    CTGATCCAGG ACTACTTCCG CAAATTCCGG CGGAGGAAAG AAAAGGGGCT ACTAGGCACC
    GAGGCCCCCC CAAGCACCTC CTCTGCCCTT CAGGCTGGCC TGAGGAGCCT GCAAGACTTG
    GGTCCGGAGA TGCGGCAGGC CCTCACTTGT GACACAGAGG AAGAGGAAGG GGAAGAGGGG
    CAGGAGGGAG AGGAGGAACA CGAGGACCTG GAAACGTACA CAGCCCTGAT GGGCTCCCAG
    CCCCCATCTC GCCGGAGCTC CGTGATTTCT GTGTCTCTAC CTGCTGGAGA CAAACCTCCA
    GATTCACTTT CCCCTGGACC CAGTGATGAC GATGGGGGGG CTCCCAACTC CAGACAGTCC
    AATGTTCCCC AGTCTGGGTC CCATGCCCAC AGGAGAAGCT CTGGGGTTCT CATTTTCACC
    ATTCCAGAAG AAAGAAGTTC TCAGTCCAAG GCAACCAAAG GGCAAGAGAA GCAGGATGAG
    CAAGAGGAAC CATTCCTCAT GGACAGGCTC TCCTACCTAG ATGAACAGGC AGGGGCTCCC
    CCACGCCCCA TCCTTTTGCC ATCTCACAGA CCCCAGAGAT ACGTGGATGG CCGCCAAGCA
    CCACGCCGCC GTCTGCTGCC CCCAACGCCT GCAGGTCGGA AGCCATCCTT CACCATCCAG
    TGTCTGCGGC GCCAGGGCAG CTGTGAAGAT TTACCCATCC CAGGCACCTA TCATCGTGGA
    CGAAACTCAG GGCCCAGTAG GGCACAGGGT TCCTGGGCAA CCCCTCCTCA GCGGGGCCGA
    CTCCTATATG CCCCACTGCT ATTGGTGGAG GAGGGTGCAG CAGGGGAGGG ATACATCGGC
    AAATCCAGCA GTCCACTGCG CACCTTCACC TGTCTGCATG TGCCTGGAAC CCACTTGGAC
    CCCAGCCATG GCAAAAGGGG CAGTGCTGAC AGCCTGGTGG AGGCTGTGCT CATCTCTGAG
    GGCCTGGGCC TCTTTGCCCG AGACCCACGC TTCGTGGCCC TGGCCAAGCA GGAGATTGCA
    GATGCATGTC GCCTGACCCT GGACGAGATG GACAGTGCTG CCAGTGACCT GCTGGCACAG
    GGGACCAGCT CACTGTATAG TGACGAAGAG TCCATCCTCT CCCGTTTTGA TGAGGAGGAC
    CTAGGAGACG AGATGGCCTG CGTCCATGCC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006073714.1
    CDS1..5916
    Translation

    Target ORF information:

    RefSeq Version XM_006073652.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006073652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG
    GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTGCGCTGGT TCAGTCACTC CACCCACTCC
    ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA
    GACGAGGAAG AGGAAGAGGG CACTCTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCATG
    AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG
    AGGGCCGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT
    CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT
    GCCAACAAAG TGCTACTCTG TCTGTTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG
    GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG
    GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA
    CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC
    AATTTAGTGG CATCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC
    TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC
    TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT
    GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC
    TATGGCGGCC CCTTCTTCCC AGGGATGCTG GTGTGCGTGT ATTTCATCAT CCTCTTCATC
    TGTGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT
    GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG
    GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA
    GGAGCAGACA TGGAGGAAGA GGAGGAGGAG GAGGAAGAGG AGGAAGAGGA AGTGGGGGCC
    GGGCATGTGG AACTCCTGCA GGAAGTTGTA CCCAAGGAGA AGGTGGTGCC CATTCCTGAG
    GGCAGTGCTT TCTTCTGCCT CAGCCAAACC AACCCGCTGA GGAAGGCCTG CCACACCCTC
    ATCCACCATC ACATCTTCAC CAATCTCATC CTGGTGTTTA TCATCCTCAG CAGTGTGTCC
    CTGGCCGCTG AGGATCCCAT CCGAGCCCAC TCCTTCCGCA ACCACATTCT GGGGTACTTC
    GATTATGCCT TCACCTCCAT TTTCACTGTG GAGATTCTAC TAAAGATGAC AGTGTTCGGG
    GCTTTTCTGC ACCGTGGTTC CTTCTGCCGT AGCTGGTTCA ATCTGCTGGA TCTGCTGGTG
    GTCAGCGTGT CCCTCATCTC CTTTGGCATC CACTCCAGCG CCATCTCAGT GGTGAAGATT
    CTGCGTGTAC TCCGAGTACT GCGGCCCCTT CGAGCTATCA ACAGGGCCAA AGGACTCAAG
    CACGTGGTGC AATGTGTGTT CGTGGCCATC CGGACCATCG GAAATATCAT GATCGTGACC
    ACGCTCCTGC AGTTCATGTT CGCCTGCATT GGTGTGCAGC TCTTCAAGGG GAAATTCTAC
    AGTTGCACTG ATGAGGCCAA ACACACACCC CAAGAATGCA AGGGCTCCTT CCTGGTCTAC
    CCCGATGGAG ATGTGTCAAG GCCTCTGGTC CGGGAGCGGC TCTGGGTCAA CAGCGATTTC
    AACTTTGACA ATGTCCTTTC AGCCATGATG GCCCTGTTCA CTGTCTCCAC CTTCGAAGGC
    TGGCCTGCGC TGCTATACAA GGCCATTGAT GCAAACGCAG AGGACAAGGG CCCCATCTAC
    AATTACCACG TGGAGATCTC AGTGTTCTTC ATTGTCTACA TCATCATCAT TGCATTCTTC
    ATGATGAACA TCTTTGTGGG CTTTGTCATC ATCACCTTCC GAGCCCAGGG CGAGCAGGAG
    TACCAAGACT GTGAGCTGGA TAAGAACCAG CGCCAGTGTG TAGAATACGC CCTCAAGGCC
    CAACCACTCC GTCGCTATAT CCCCAAGAAC CCACATCAGT ATCGTGTGTG GGCCACTGTG
    AACTCTGCTG CCTTCGAGTA TCTCATGTTC CTGCTCATCC TGCTCAACAC GGTTGCTCTA
    GCCATGCAGC ACTATGAGCA GACTGCTCCC TTCAACTATG CCATGGACAT CCTCAACATG
    GTCTTCACTG GCCTCTTCAC TGTTGAGATG GTGCTCAAAA TCATTGCCTT CAAACCCAAG
    CATTACTTTA CTGATGCCTG GAACACATTT GATGCTCTTA TTGTGGTGGG CAGCGTAGTG
    GACATTGCCG TCACTGAAGT CAATAATGGT GGCCACCTGG GCGAGAGCTC TGAGGATAGT
    TCCCGCATTT CGATCACCTT CTTTCGCCTC TTCCGAGTCA TGCGATTGGT CAAGCTTCTT
    AGTAAGGGTG AAGGGATTCG CACATTACTT TGGACATTTA TCAAGTCTTT TCAGGCCTTA
    CCCTATGTGG CTCTTCTCAT CGCAATGATA TTCTTCATTT ACGCAGTCAT TGGGATGCAG
    ATGTTTGGCA AGGTGGCTCT TCAGGATGGC ACACAGATCA ACCGAAACAA CAACTTCCAG
    ACCTTTCCAC AGGCTGTACT GCTTTTGTTC AGGTGTGCTA CTGGTGAGGC ATGGCAGGAG
    ATAATGCTTG CCAGCCTTCC TGGAAGCCGG TGTGACCCTG AGTCTGACTT CGGCCCTGGT
    GAGGAGTTTA GCTGTGGTAG CAATTTTGCC ATCGCCTATT TTATCAGCTT CTTCATGCTC
    TGTGCATTCC TGATCATAAA TCTCTTTGTG GCTGTGATCA TGGACAACTT TGATTATCTA
    ACCAGAGATT GGTCCATCCT GGGCCCCCAT CACCTCGATG AATTCAAGAG GATTTGGTCT
    GAATATGACC CTGGGGCCAA AGGCCGCATC AAGCACCTGG ATGTGGTTGC CCTGCTGAGA
    CGCATTCAGC CCCCCCTGGG ATTCGGGAAG CTGTGCCCGC ACCGAGTGGC CTGCAAGAGA
    CTTGTGGCGA TGAACATGCC CCTCAACTCA GATGGGACAG TGACATTCAA TGCCACACTC
    TTTGCCCTGG TCCGGACATC ACTGAAGATC AAGACGGAAG GAAACCTGGA GCAAGCCAAT
    CAGGAGCTGC GGATTGTCAT CAAAAAGATC TGGAAGCGGA TGAAGCAGAA GCTGCTAGAT
    GAGGTCATCC CACCAGCTGA TGAGGAAGAA GTTACCGTGG GCAAATTCTA CGCCACATTT
    CTGATCCAGG ACTACTTCCG CAAATTCCGG CGGAGGAAAG AAAAGGGGCT ACTAGGCACC
    GAGGCCCCCC CAAGCACCTC CTCTGCCCTT CAGGCTGGCC TGAGGAGCCT GCAAGACTTG
    GGTCCGGAGA TGCGGCAGGC CCTCACTTGT GACACAGAGG AAGAGGAAGG GGAAGAGGGG
    CAGGAGGGAG AGGAGGAACA CGAGGACCTG GAAACGTACA CAGCCCTGAT GGGCTCCCAG
    CCCCCATCTC GCCGGAGCTC CGTGATTTCT GTGTCTCTAC CTGCTGGAGA CAAACCTCCA
    GATTCACTTT CCCCTGGACC CAGTGATGAC GATGGGGGGG CTCCCAACTC CAGACAGTCC
    AATGTTCCCC AGTCTGGGTC CCATGCCCAC AGGAGAAGCT CTGGGGTTCT CATTTTCACC
    ATTCCAGAAG AAAGAAGTTC TCAGTCCAAG GCAACCAAAG GGCAAGAGAA GCAGGATGAG
    CAAGAGGAAC CATTCCTCAT GGACAGGCTC TCCTACCTAG ATGAACAGGC AGGGGCTCCC
    CCACGCCCCA TCCTTTTGCC ATCTCACAGA CCCCAGAGAT ACGTGGATGG CCGCCAAGCA
    CCACGCCGCC GTCTGCTGCC CCCAACGCCT GCAGGTCGGA AGCCATCCTT CACCATCCAG
    TGTCTGCGGC GCCAGGGCAG CTGTGAAGAT TTACCCATCC CAGGCACCTA TCATCGTGGA
    CGAAACTCAG GGCCCAGTAG GGCACAGGGT TCCTGGGCAA CCCCTCCTCA GCGGGGCCGA
    CTCCTATATG CCCCACTGCT ATTGGTGGAG GAGGGTGCAG CAGGGGAGGG ATACATCGGC
    AAATCCAGCA GTCCACTGCG CACCTTCACC TGTCTGCATG TGCCTGGAAC CCACTTGGAC
    CCCAGCCATG GCAAAAGGGG CAGTGCTGAC AGCCTGGTGG AGGCTGTGCT CATCTCTGAG
    GGCCTGGGCC TCTTTGCCCG AGACCCACGC TTCGTGGCCC TGGCCAAGCA GGAGATTGCA
    GATGCATGTC GCCTGACCCT GGACGAGATG GACAGTGCTG CCAGTGACCT GCTGGCACAG
    GGGACCAGCT CACTGTATAG TGACGAAGAG TCCATCCTCT CCCGTTTTGA TGAGGAGGAC
    CTAGGAGACG AGATGGCCTG CGTCCATGCC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu27684
    Clone ID Related Accession (Same CDS sequence) XM_006073653.1
    Accession Version XM_006073653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGGCC ACAACTGGAA GAGCTGACCA ATAGGAGACG AGGACGTCTG
    CGCTGGTTCA GTCACTCCAC CCACTCCACC AGCAGCCATG CCAGCCTCCC AGCCAGTGAC
    ACCGGTTCAA TGGCAGAGAC CCAAGGAGAC GAGGAAGAGG AAGAGGGCAC TCTAGCCAGC
    TGTACACGCT GCCTAAATAA GATCATGAAA ACCAGCGTTT GCCGCCGTCT CCGCCGAGCC
    AACCGGGGCC TTCGGGCGAG CTGCCGGAGG GCCGTGAAGT CCAATGCCTG CTACTGGGCC
    GTGTTGCTGC TCGTCTTCCT CAACACTCTG ACAATCGCCT CGGAGCACCA CGGGCAGCCC
    TTCTGGCTCA CCCAGATCCA GGAGTATGCC AACAAAGTGC TACTCTGTCT GTTCACGGTG
    GAGATGCTTC TCAAGTTGTA TGGTCTGGGG CCCTCTGTCT ACGTGTCCTC CTTCTTCAAC
    CGCTTTGACT GCTTCGTGGT CTGTGGGGGC ATCCTGGAGA CTACCCTGGT GGAGGTGGGT
    GCCATGCAGC CCCTGGGCAT CTCGGTACTC CGCTGTGTGC GCCTCCTCAG GATCTTTAAG
    GTCACCAGGC ACTGGGCATC TCTGAGCAAT TTAGTGGCAT CTCTACTCAA TTCAATGAAA
    TCCATTGCAT CCTTGCTGCT TCTCCTCTTC CTCTTTATCA TCATCTTCTC CCTGCTTGGC
    ATGCAGCTGT TTGGGGGCAA GTTCAACTTT GACCAGACCC ACACCAAGCG AAGCACCTTT
    GACACCTTTC CCCAGGCCCT CCTCACTGTC TTTCAGATAC TGACGGGTGA GGACTGGAAT
    GTGGTCATGT ATGATGGTAT CATGGCCTAT GGCGGCCCCT TCTTCCCAGG GATGCTGGTG
    TGCGTGTATT TCATCATCCT CTTCATCTGT GGCAATTACA TCCTATTGAA TGTGTTTCTT
    GCCATCGCTG TGGACAACCT GGCCAGTGGA GATGCAGGCA CTGACAAAGA TAAGGGCAGG
    GAGAAGATCA CTGAGGAAAC TCCACAGGAG AATGGAGCAC TGGTGCCTGG TGGGGAGAAT
    GAGGAAGAGG AATCTGCAAA AAATGAAGGA GCAGACATGG AGGAAGAGGA GGAGGAGGAG
    GAAGAGGAGG AAGAGGAAGT GGGGGCCGGG CATGTGGAAC TCCTGCAGGA AGTTGTACCC
    AAGGAGAAGG TGGTGCCCAT TCCTGAGGGC AGTGCTTTCT TCTGCCTCAG CCAAACCAAC
    CCGCTGAGGA AGGCCTGCCA CACCCTCATC CACCATCACA TCTTCACCAA TCTCATCCTG
    GTGTTTATCA TCCTCAGCAG TGTGTCCCTG GCCGCTGAGG ATCCCATCCG AGCCCACTCC
    TTCCGCAACC ACATTCTGGG GTACTTCGAT TATGCCTTCA CCTCCATTTT CACTGTGGAG
    ATTCTACTAA AGATGACAGT GTTCGGGGCT TTTCTGCACC GTGGTTCCTT CTGCCGTAGC
    TGGTTCAATC TGCTGGATCT GCTGGTGGTC AGCGTGTCCC TCATCTCCTT TGGCATCCAC
    TCCAGCGCCA TCTCAGTGGT GAAGATTCTG CGTGTACTCC GAGTACTGCG GCCCCTTCGA
    GCTATCAACA GGGCCAAAGG ACTCAAGCAC GTGGTGCAAT GTGTGTTCGT GGCCATCCGG
    ACCATCGGAA ATATCATGAT CGTGACCACG CTCCTGCAGT TCATGTTCGC CTGCATTGGT
    GTGCAGCTCT TCAAGGGGAA ATTCTACAGT TGCACTGATG AGGCCAAACA CACACCCCAA
    GAATGCAAGG GCTCCTTCCT GGTCTACCCC GATGGAGATG TGTCAAGGCC TCTGGTCCGG
    GAGCGGCTCT GGGTCAACAG CGATTTCAAC TTTGACAATG TCCTTTCAGC CATGATGGCC
    CTGTTCACTG TCTCCACCTT CGAAGGCTGG CCTGCGCTGC TATACAAGGC CATTGATGCA
    AACGCAGAGG ACAAGGGCCC CATCTACAAT TACCACGTGG AGATCTCAGT GTTCTTCATT
    GTCTACATCA TCATCATTGC ATTCTTCATG ATGAACATCT TTGTGGGCTT TGTCATCATC
    ACCTTCCGAG CCCAGGGCGA GCAGGAGTAC CAAGACTGTG AGCTGGATAA GAACCAGCGC
    CAGTGTGTAG AATACGCCCT CAAGGCCCAA CCACTCCGTC GCTATATCCC CAAGAACCCA
    CATCAGTATC GTGTGTGGGC CACTGTGAAC TCTGCTGCCT TCGAGTATCT CATGTTCCTG
    CTCATCCTGC TCAACACGGT TGCTCTAGCC ATGCAGCACT ATGAGCAGAC TGCTCCCTTC
    AACTATGCCA TGGACATCCT CAACATGGTC TTCACTGGCC TCTTCACTGT TGAGATGGTG
    CTCAAAATCA TTGCCTTCAA ACCCAAGCAT TACTTTACTG ATGCCTGGAA CACATTTGAT
    GCTCTTATTG TGGTGGGCAG CGTAGTGGAC ATTGCCGTCA CTGAAGTCAA TAATGGTGGC
    CACCTGGGCG AGAGCTCTGA GGATAGTTCC CGCATTTCGA TCACCTTCTT TCGCCTCTTC
    CGAGTCATGC GATTGGTCAA GCTTCTTAGT AAGGGTGAAG GGATTCGCAC ATTACTTTGG
    ACATTTATCA AGTCTTTTCA GGCCTTACCC TATGTGGCTC TTCTCATCGC AATGATATTC
    TTCATTTACG CAGTCATTGG GATGCAGATG TTTGGCAAGG TGGCTCTTCA GGATGGCACA
    CAGATCAACC GAAACAACAA CTTCCAGACC TTTCCACAGG CTGTACTGCT TTTGTTCAGG
    TGTGCTACTG GTGAGGCATG GCAGGAGATA ATGCTTGCCA GCCTTCCTGG AAGCCGGTGT
    GACCCTGAGT CTGACTTCGG CCCTGGTGAG GAGTTTAGCT GTGGTAGCAA TTTTGCCATC
    GCCTATTTTA TCAGCTTCTT CATGCTCTGT GCATTCCTGA TCATAAATCT CTTTGTGGCT
    GTGATCATGG ACAACTTTGA TTATCTAACC AGAGATTGGT CCATCCTGGG CCCCCATCAC
    CTCGATGAAT TCAAGAGGAT TTGGTCTGAA TATGACCCTG GGGCCAAAGG CCGCATCAAG
    CACCTGGATG TGGTTGCCCT GCTGAGACGC ATTCAGCCCC CCCTGGGATT CGGGAAGCTG
    TGCCCGCACC GAGTGGCCTG CAAGAGACTT GTGGCGATGA ACATGCCCCT CAACTCAGAT
    GGGACAGTGA CATTCAATGC CACACTCTTT GCCCTGGTCC GGACATCACT GAAGATCAAG
    ACGGAAGGAA ACCTGGAGCA AGCCAATCAG GAGCTGCGGA TTGTCATCAA AAAGATCTGG
    AAGCGGATGA AGCAGAAGCT GCTAGATGAG GTCATCCCAC CAGCTGATGA GGAAGAAGTT
    ACCGTGGGCA AATTCTACGC CACATTTCTG ATCCAGGACT ACTTCCGCAA ATTCCGGCGG
    AGGAAAGAAA AGGGGCTACT AGGCACCGAG GCCCCCCCAA GCACCTCCTC TGCCCTTCAG
    GCTGGCCTGA GGAGCCTGCA AGACTTGGGT CCGGAGATGC GGCAGGCCCT CACTTGTGAC
    ACAGAGGAAG AGGAAGGGGA AGAGGGGCAG GAGGGAGAGG AGGAACACGA GGACCTGGAA
    ACGTACACAG CCCTGATGGG CTCCCAGCCC CCATCTCGCC GGAGCTCCGT GATTTCTGTG
    TCTCTACCTG CTGGAGACAA ACCTCCAGAT TCACTTTCCC CTGGACCCAG TGATGACGAT
    GGGGGGGCTC CCAACTCCAG ACAGTCCAAT GTTCCCCAGT CTGGGTCCCA TGCCCACAGG
    AGAAGCTCTG GGGTTCTCAT TTTCACCATT CCAGAAGAAA GAAGTTCTCA GTCCAAGGCA
    ACCAAAGGGC AAGAGAAGCA GGATGAGCAA GAGGAACCAT TCCTCATGGA CAGGCTCTCC
    TACCTAGATG AACAGGCAGG GGCTCCCCCA CGCCCCATCC TTTTGCCATC TCACAGACCC
    CAGAGATACG TGGATGGCCG CCAAGCACCA CGCCGCCGTC TGCTGCCCCC AACGCCTGCA
    GGTCGGAAGC CATCCTTCAC CATCCAGTGT CTGCGGCGCC AGGGCAGCTG TGAAGATTTA
    CCCATCCCAG GCACCTATCA TCGTGGACGA AACTCAGGGC CCAGTAGGGC ACAGGGTTCC
    TGGGCAACCC CTCCTCAGCG GGGCCGACTC CTATATGCCC CACTGCTATT GGTGGAGGAG
    GGTGCAGCAG GGGAGGGATA CATCGGCAAA TCCAGCAGTC CACTGCGCAC CTTCACCTGT
    CTGCATGTGC CTGGAACCCA CTTGGACCCC AGCCATGGCA AAAGGGGCAG TGCTGACAGC
    CTGGTGGAGG CTGTGCTCAT CTCTGAGGGC CTGGGCCTCT TTGCCCGAGA CCCACGCTTC
    GTGGCCCTGG CCAAGCAGGA GATTGCAGAT GCATGTCGCC TGACCCTGGA CGAGATGGAC
    AGTGCTGCCA GTGACCTGCT GGCACAGGGG ACCAGCTCAC TGTATAGTGA CGAAGAGTCC
    ATCCTCTCCC GTTTTGATGA GGAGGACCTA GGAGACGAGA TGGCCTGCGT CCATGCCCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006073715.1
    CDS1..5883
    Translation

    Target ORF information:

    RefSeq Version XM_006073653.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006073653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGTCAGCAT CTGAAGGCGG GAAAGACAGC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
    GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC
    GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG
    GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC
    CTACGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG
    ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCAGA GGACGACTCC
    AACACTGCCA ACCACAACCT GGAGCAGGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG
    GAGACGGTGC TCAAGATCGT GGCCTACGGG CTGGTGCTCC ACCCCAGCGC CTACATCCGC
    AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG
    GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC
    GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGAGTC
    CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT
    GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC
    GGACGCATGC ACAAAACGTG CTATTTCCTG GGATCTGACA TAGAGGCGGA GGAGGACCCG
    TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC
    GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG
    CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG
    GATGCCATGG GGTATGAGCT GCCCTGGGTA TATTTCGTGA GTCTCGTCAT CTTTGGATCC
    TTCTTCGTCC TCAACCTTGT ACTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG
    AAGGCAAAGG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC
    CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA
    GCTGCTGGCA ACTTTGGGCC ACAACTGGAA GAGCTGACCA ATAGGAGACG AGGACGTCTG
    CGCTGGTTCA GTCACTCCAC CCACTCCACC AGCAGCCATG CCAGCCTCCC AGCCAGTGAC
    ACCGGTTCAA TGGCAGAGAC CCAAGGAGAC GAGGAAGAGG AAGAGGGCAC TCTAGCCAGC
    TGTACACGCT GCCTAAATAA GATCATGAAA ACCAGCGTTT GCCGCCGTCT CCGCCGAGCC
    AACCGGGGCC TTCGGGCGAG CTGCCGGAGG GCCGTGAAGT CCAATGCCTG CTACTGGGCC
    GTGTTGCTGC TCGTCTTCCT CAACACTCTG ACAATCGCCT CGGAGCACCA CGGGCAGCCC
    TTCTGGCTCA CCCAGATCCA GGAGTATGCC AACAAAGTGC TACTCTGTCT GTTCACGGTG
    GAGATGCTTC TCAAGTTGTA TGGTCTGGGG CCCTCTGTCT ACGTGTCCTC CTTCTTCAAC
    CGCTTTGACT GCTTCGTGGT CTGTGGGGGC ATCCTGGAGA CTACCCTGGT GGAGGTGGGT
    GCCATGCAGC CCCTGGGCAT CTCGGTACTC CGCTGTGTGC GCCTCCTCAG GATCTTTAAG
    GTCACCAGGC ACTGGGCATC TCTGAGCAAT TTAGTGGCAT CTCTACTCAA TTCAATGAAA
    TCCATTGCAT CCTTGCTGCT TCTCCTCTTC CTCTTTATCA TCATCTTCTC CCTGCTTGGC
    ATGCAGCTGT TTGGGGGCAA GTTCAACTTT GACCAGACCC ACACCAAGCG AAGCACCTTT
    GACACCTTTC CCCAGGCCCT CCTCACTGTC TTTCAGATAC TGACGGGTGA GGACTGGAAT
    GTGGTCATGT ATGATGGTAT CATGGCCTAT GGCGGCCCCT TCTTCCCAGG GATGCTGGTG
    TGCGTGTATT TCATCATCCT CTTCATCTGT GGCAATTACA TCCTATTGAA TGTGTTTCTT
    GCCATCGCTG TGGACAACCT GGCCAGTGGA GATGCAGGCA CTGACAAAGA TAAGGGCAGG
    GAGAAGATCA CTGAGGAAAC TCCACAGGAG AATGGAGCAC TGGTGCCTGG TGGGGAGAAT
    GAGGAAGAGG AATCTGCAAA AAATGAAGGA GCAGACATGG AGGAAGAGGA GGAGGAGGAG
    GAAGAGGAGG AAGAGGAAGT GGGGGCCGGG CATGTGGAAC TCCTGCAGGA AGTTGTACCC
    AAGGAGAAGG TGGTGCCCAT TCCTGAGGGC AGTGCTTTCT TCTGCCTCAG CCAAACCAAC
    CCGCTGAGGA AGGCCTGCCA CACCCTCATC CACCATCACA TCTTCACCAA TCTCATCCTG
    GTGTTTATCA TCCTCAGCAG TGTGTCCCTG GCCGCTGAGG ATCCCATCCG AGCCCACTCC
    TTCCGCAACC ACATTCTGGG GTACTTCGAT TATGCCTTCA CCTCCATTTT CACTGTGGAG
    ATTCTACTAA AGATGACAGT GTTCGGGGCT TTTCTGCACC GTGGTTCCTT CTGCCGTAGC
    TGGTTCAATC TGCTGGATCT GCTGGTGGTC AGCGTGTCCC TCATCTCCTT TGGCATCCAC
    TCCAGCGCCA TCTCAGTGGT GAAGATTCTG CGTGTACTCC GAGTACTGCG GCCCCTTCGA
    GCTATCAACA GGGCCAAAGG ACTCAAGCAC GTGGTGCAAT GTGTGTTCGT GGCCATCCGG
    ACCATCGGAA ATATCATGAT CGTGACCACG CTCCTGCAGT TCATGTTCGC CTGCATTGGT
    GTGCAGCTCT TCAAGGGGAA ATTCTACAGT TGCACTGATG AGGCCAAACA CACACCCCAA
    GAATGCAAGG GCTCCTTCCT GGTCTACCCC GATGGAGATG TGTCAAGGCC TCTGGTCCGG
    GAGCGGCTCT GGGTCAACAG CGATTTCAAC TTTGACAATG TCCTTTCAGC CATGATGGCC
    CTGTTCACTG TCTCCACCTT CGAAGGCTGG CCTGCGCTGC TATACAAGGC CATTGATGCA
    AACGCAGAGG ACAAGGGCCC CATCTACAAT TACCACGTGG AGATCTCAGT GTTCTTCATT
    GTCTACATCA TCATCATTGC ATTCTTCATG ATGAACATCT TTGTGGGCTT TGTCATCATC
    ACCTTCCGAG CCCAGGGCGA GCAGGAGTAC CAAGACTGTG AGCTGGATAA GAACCAGCGC
    CAGTGTGTAG AATACGCCCT CAAGGCCCAA CCACTCCGTC GCTATATCCC CAAGAACCCA
    CATCAGTATC GTGTGTGGGC CACTGTGAAC TCTGCTGCCT TCGAGTATCT CATGTTCCTG
    CTCATCCTGC TCAACACGGT TGCTCTAGCC ATGCAGCACT ATGAGCAGAC TGCTCCCTTC
    AACTATGCCA TGGACATCCT CAACATGGTC TTCACTGGCC TCTTCACTGT TGAGATGGTG
    CTCAAAATCA TTGCCTTCAA ACCCAAGCAT TACTTTACTG ATGCCTGGAA CACATTTGAT
    GCTCTTATTG TGGTGGGCAG CGTAGTGGAC ATTGCCGTCA CTGAAGTCAA TAATGGTGGC
    CACCTGGGCG AGAGCTCTGA GGATAGTTCC CGCATTTCGA TCACCTTCTT TCGCCTCTTC
    CGAGTCATGC GATTGGTCAA GCTTCTTAGT AAGGGTGAAG GGATTCGCAC ATTACTTTGG
    ACATTTATCA AGTCTTTTCA GGCCTTACCC TATGTGGCTC TTCTCATCGC AATGATATTC
    TTCATTTACG CAGTCATTGG GATGCAGATG TTTGGCAAGG TGGCTCTTCA GGATGGCACA
    CAGATCAACC GAAACAACAA CTTCCAGACC TTTCCACAGG CTGTACTGCT TTTGTTCAGG
    TGTGCTACTG GTGAGGCATG GCAGGAGATA ATGCTTGCCA GCCTTCCTGG AAGCCGGTGT
    GACCCTGAGT CTGACTTCGG CCCTGGTGAG GAGTTTAGCT GTGGTAGCAA TTTTGCCATC
    GCCTATTTTA TCAGCTTCTT CATGCTCTGT GCATTCCTGA TCATAAATCT CTTTGTGGCT
    GTGATCATGG ACAACTTTGA TTATCTAACC AGAGATTGGT CCATCCTGGG CCCCCATCAC
    CTCGATGAAT TCAAGAGGAT TTGGTCTGAA TATGACCCTG GGGCCAAAGG CCGCATCAAG
    CACCTGGATG TGGTTGCCCT GCTGAGACGC ATTCAGCCCC CCCTGGGATT CGGGAAGCTG
    TGCCCGCACC GAGTGGCCTG CAAGAGACTT GTGGCGATGA ACATGCCCCT CAACTCAGAT
    GGGACAGTGA CATTCAATGC CACACTCTTT GCCCTGGTCC GGACATCACT GAAGATCAAG
    ACGGAAGGAA ACCTGGAGCA AGCCAATCAG GAGCTGCGGA TTGTCATCAA AAAGATCTGG
    AAGCGGATGA AGCAGAAGCT GCTAGATGAG GTCATCCCAC CAGCTGATGA GGAAGAAGTT
    ACCGTGGGCA AATTCTACGC CACATTTCTG ATCCAGGACT ACTTCCGCAA ATTCCGGCGG
    AGGAAAGAAA AGGGGCTACT AGGCACCGAG GCCCCCCCAA GCACCTCCTC TGCCCTTCAG
    GCTGGCCTGA GGAGCCTGCA AGACTTGGGT CCGGAGATGC GGCAGGCCCT CACTTGTGAC
    ACAGAGGAAG AGGAAGGGGA AGAGGGGCAG GAGGGAGAGG AGGAACACGA GGACCTGGAA
    ACGTACACAG CCCTGATGGG CTCCCAGCCC CCATCTCGCC GGAGCTCCGT GATTTCTGTG
    TCTCTACCTG CTGGAGACAA ACCTCCAGAT TCACTTTCCC CTGGACCCAG TGATGACGAT
    GGGGGGGCTC CCAACTCCAG ACAGTCCAAT GTTCCCCAGT CTGGGTCCCA TGCCCACAGG
    AGAAGCTCTG GGGTTCTCAT TTTCACCATT CCAGAAGAAA GAAGTTCTCA GTCCAAGGCA
    ACCAAAGGGC AAGAGAAGCA GGATGAGCAA GAGGAACCAT TCCTCATGGA CAGGCTCTCC
    TACCTAGATG AACAGGCAGG GGCTCCCCCA CGCCCCATCC TTTTGCCATC TCACAGACCC
    CAGAGATACG TGGATGGCCG CCAAGCACCA CGCCGCCGTC TGCTGCCCCC AACGCCTGCA
    GGTCGGAAGC CATCCTTCAC CATCCAGTGT CTGCGGCGCC AGGGCAGCTG TGAAGATTTA
    CCCATCCCAG GCACCTATCA TCGTGGACGA AACTCAGGGC CCAGTAGGGC ACAGGGTTCC
    TGGGCAACCC CTCCTCAGCG GGGCCGACTC CTATATGCCC CACTGCTATT GGTGGAGGAG
    GGTGCAGCAG GGGAGGGATA CATCGGCAAA TCCAGCAGTC CACTGCGCAC CTTCACCTGT
    CTGCATGTGC CTGGAACCCA CTTGGACCCC AGCCATGGCA AAAGGGGCAG TGCTGACAGC
    CTGGTGGAGG CTGTGCTCAT CTCTGAGGGC CTGGGCCTCT TTGCCCGAGA CCCACGCTTC
    GTGGCCCTGG CCAAGCAGGA GATTGCAGAT GCATGTCGCC TGACCCTGGA CGAGATGGAC
    AGTGCTGCCA GTGACCTGCT GGCACAGGGG ACCAGCTCAC TGTATAGTGA CGAAGAGTCC
    ATCCTCTCCC GTTTTGATGA GGAGGACCTA GGAGACGAGA TGGCCTGCGT CCATGCCCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu27685
    Clone ID Related Accession (Same CDS sequence) XM_006073654.1
    Accession Version XM_006073654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product voltage-dependent L-type calcium channel subunit alpha-1F isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCAGCAT CTGAAGGCGG GAAAGGTGAG AGCAACCTTC CACCCCTACA GACCCTTGGA 
    GCAAGCCTGG ACTCTTGGCA GCCCTTCGAC ATCCTCATCT TGCTGACCAT CTTTGCCAAT
    TGCGTGGCCC TGGGAGTCTA CATCCCCTTC CCAGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAATA CGTATTTTTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAACGG CTGGAACCTG
    CTCGACTTCA TCATTGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAGCA GGGTCCCGGA
    CGCCCAGGGG ACACCCCGCA TATGGGAGGG AAGCCAGGGG GCTTCGATGT GAAGGCATTG
    CGGGCATTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GAGTCCCAAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGTTGC ATATTGCACT TCTTGTGCTC
    TTTGTCATCA TCATTTACGC CATCATTGGA CTGGAGCTGT TCCTCGGACG CATGCACAAA
    ACGTGCTATT TCCTGGGATC TGACATAGAG GCGGAGGAGG ACCCGTCACC CTGTGCGTCC
    TCAGGATCAG GGCGTGCTTG CACACTGAAC CAGACGGAGT GCCGCGGGCG CTGGGCAGGG
    CCCAACGGAG GCATCACCAA CTTTGACAAC TTTTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGCATCACCA TGGAAGGCTG GACCGACGTG CTCTACTGGA TGCAGGATGC CATGGGGTAT
    GAGCTGCCCT GGGTATATTT CGTGAGTCTC GTCATCTTTG GATCCTTCTT CGTCCTCAAC
    CTTGTACTTG GTGTCCTGAG TGGGGAGTTC TCCAAAGAGA GGGAGAAGGC AAAGGCACGA
    GGGGATTTCC AGAAGCTTCG GGAGAAGCAG CAGCTGGAGG AGGACCTCCG GGGCTACCTG
    GACTGGATCA CACAGGCGGA GGAGCTGGAC ATGGAGGACC CCTCAGCTGC TGGCAACTTT
    GGTTCCGTGG CTGAAGAGGG CCTGGCCGGC CACCGGCCAC AACTGGAAGA GCTGACCAAT
    AGGAGACGAG GACGTCTGCG CTGGTTCAGT CACTCCACCC ACTCCACCAG CAGCCATGCC
    AGCCTCCCAG CCAGTGACAC CGGTTCAATG GCAGAGACCC AAGGAGACGA GGAAGAGGAA
    GAGGGCACTC TAGCCAGCTG TACACGCTGC CTAAATAAGA TCATGAAAAC CAGCGTTTGC
    CGCCGTCTCC GCCGAGCCAA CCGGGGCCTT CGGGCGAGCT GCCGGAGGGC CGTGAAGTCC
    AATGCCTGCT ACTGGGCCGT GTTGCTGCTC GTCTTCCTCA ACACTCTGAC AATCGCCTCG
    GAGCACCACG GGCAGCCCTT CTGGCTCACC CAGATCCAGG AGTATGCCAA CAAAGTGCTA
    CTCTGTCTGT TCACGGTGGA GATGCTTCTC AAGTTGTATG GTCTGGGGCC CTCTGTCTAC
    GTGTCCTCCT TCTTCAACCG CTTTGACTGC TTCGTGGTCT GTGGGGGCAT CCTGGAGACT
    ACCCTGGTGG AGGTGGGTGC CATGCAGCCC CTGGGCATCT CGGTACTCCG CTGTGTGCGC
    CTCCTCAGGA TCTTTAAGGT CACCAGGCAC TGGGCATCTC TGAGCAATTT AGTGGCATCT
    CTACTCAATT CAATGAAATC CATTGCATCC TTGCTGCTTC TCCTCTTCCT CTTTATCATC
    ATCTTCTCCC TGCTTGGCAT GCAGCTGTTT GGGGGCAAGT TCAACTTTGA CCAGACCCAC
    ACCAAGCGAA GCACCTTTGA CACCTTTCCC CAGGCCCTCC TCACTGTCTT TCAGATACTG
    ACGGGTGAGG ACTGGAATGT GGTCATGTAT GATGGTATCA TGGCCTATGG CGGCCCCTTC
    TTCCCAGGGA TGCTGGTGTG CGTGTATTTC ATCATCCTCT TCATCTGTGG CAATTACATC
    CTATTGAATG TGTTTCTTGC CATCGCTGTG GACAACCTGG CCAGTGGAGA TGCAGGCACT
    GACAAAGATA AGGGCAGGGA GAAGATCACT GAGGAAACTC CACAGGAGAA TGGAGCACTG
    GTGCCTGGTG GGGAGAATGA GGAAGAGGAA TCTGCAAAAA ATGAAGGAGC AGACATGGAG
    GAAGAGGAGG AGGAGGAGGA AGAGGAGGAA GAGGAAGTGG GGGCCGGGCA TGTGGAACTC
    CTGCAGGAAG TTGTACCCAA GGAGAAGGTG GTGCCCATTC CTGAGGGCAG TGCTTTCTTC
    TGCCTCAGCC AAACCAACCC GCTGAGGAAG GCCTGCCACA CCCTCATCCA CCATCACATC
    TTCACCAATC TCATCCTGGT GTTTATCATC CTCAGCAGTG TGTCCCTGGC CGCTGAGGAT
    CCCATCCGAG CCCACTCCTT CCGCAACCAC ATTCTGGGGT ACTTCGATTA TGCCTTCACC
    TCCATTTTCA CTGTGGAGAT TCTACTAAAG ATGACAGTGT TCGGGGCTTT TCTGCACCGT
    GGTTCCTTCT GCCGTAGCTG GTTCAATCTG CTGGATCTGC TGGTGGTCAG CGTGTCCCTC
    ATCTCCTTTG GCATCCACTC CAGCGCCATC TCAGTGGTGA AGATTCTGCG TGTACTCCGA
    GTACTGCGGC CCCTTCGAGC TATCAACAGG GCCAAAGGAC TCAAGCACGT GGTGCAATGT
    GTGTTCGTGG CCATCCGGAC CATCGGAAAT ATCATGATCG TGACCACGCT CCTGCAGTTC
    ATGTTCGCCT GCATTGGTGT GCAGCTCTTC AAGGGGAAAT TCTACAGTTG CACTGATGAG
    GCCAAACACA CACCCCAAGA ATGCAAGGGC TCCTTCCTGG TCTACCCCGA TGGAGATGTG
    TCAAGGCCTC TGGTCCGGGA GCGGCTCTGG GTCAACAGCG ATTTCAACTT TGACAATGTC
    CTTTCAGCCA TGATGGCCCT GTTCACTGTC TCCACCTTCG AAGGCTGGCC TGCGCTGCTA
    TACAAGGCCA TTGATGCAAA CGCAGAGGAC AAGGGCCCCA TCTACAATTA CCACGTGGAG
    ATCTCAGTGT TCTTCATTGT CTACATCATC ATCATTGCAT TCTTCATGAT GAACATCTTT
    GTGGGCTTTG TCATCATCAC CTTCCGAGCC CAGGGCGAGC AGGAGTACCA AGACTGTGAG
    CTGGATAAGA ACCAGCGCCA GTGTGTAGAA TACGCCCTCA AGGCCCAACC ACTCCGTCGC
    TATATCCCCA AGAACCCACA TCAGTATCGT GTGTGGGCCA CTGTGAACTC TGCTGCCTTC
    GAGTATCTCA TGTTCCTGCT CATCCTGCTC AACACGGTTG CTCTAGCCAT GCAGCACTAT
    GAGCAGACTG CTCCCTTCAA CTATGCCATG GACATCCTCA ACATGGTCTT CACTGGCCTC
    TTCACTGTTG AGATGGTGCT CAAAATCATT GCCTTCAAAC CCAAGCATTA CTTTACTGAT
    GCCTGGAACA CATTTGATGC TCTTATTGTG GTGGGCAGCG TAGTGGACAT TGCCGTCACT
    GAAGTCAATA ATGGTGGCCA CCTGGGCGAG AGCTCTGAGG ATAGTTCCCG CATTTCGATC
    ACCTTCTTTC GCCTCTTCCG AGTCATGCGA TTGGTCAAGC TTCTTAGTAA GGGTGAAGGG
    ATTCGCACAT TACTTTGGAC ATTTATCAAG TCTTTTCAGG CCTTACCCTA TGTGGCTCTT
    CTCATCGCAA TGATATTCTT CATTTACGCA GTCATTGGGA TGCAGATGTT TGGCAAGGTG
    GCTCTTCAGG ATGGCACACA GATCAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT
    GTACTGCTTT TGTTCAGGTG TGCTACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC
    CTTCCTGGAA GCCGGTGTGA CCCTGAGTCT GACTTCGGCC CTGGTGAGGA GTTTAGCTGT
    GGTAGCAATT TTGCCATCGC CTATTTTATC AGCTTCTTCA TGCTCTGTGC ATTCCTGATC
    ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTAACCAG AGATTGGTCC
    ATCCTGGGCC CCCATCACCT CGATGAATTC AAGAGGATTT GGTCTGAATA TGACCCTGGG
    GCCAAAGGCC GCATCAAGCA CCTGGATGTG GTTGCCCTGC TGAGACGCAT TCAGCCCCCC
    CTGGGATTCG GGAAGCTGTG CCCGCACCGA GTGGCCTGCA AGAGACTTGT GGCGATGAAC
    ATGCCCCTCA ACTCAGATGG GACAGTGACA TTCAATGCCA CACTCTTTGC CCTGGTCCGG
    ACATCACTGA AGATCAAGAC GGAAGGAAAC CTGGAGCAAG CCAATCAGGA GCTGCGGATT
    GTCATCAAAA AGATCTGGAA GCGGATGAAG CAGAAGCTGC TAGATGAGGT CATCCCACCA
    GCTGATGAGG AAGAAGTTAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAC
    TTCCGCAAAT TCCGGCGGAG GAAAGAAAAG GGGCTACTAG GCACCGAGGC CCCCCCAAGC
    ACCTCCTCTG CCCTTCAGGC TGGCCTGAGG AGCCTGCAAG ACTTGGGTCC GGAGATGCGG
    CAGGCCCTCA CTTGTGACAC AGAGGAAGAG GAAGGGGAAG AGGGGCAGGA GGGAGAGGAG
    GAACACGAGG ACCTGGAAAC GTACACAGCC CTGATGGGCT CCCAGCCCCC ATCTCGCCGG
    AGCTCCGTGA TTTCTGTGTC TCTACCTGCT GGAGACAAAC CTCCAGATTC ACTTTCCCCT
    GGACCCAGTG ATGACGATGG GGGGGCTCCC AACTCCAGAC AGTCCAATGT TCCCCAGTCT
    GGGTCCCATG CCCACAGGAG AAGCTCTGGG GTTCTCATTT TCACCATTCC AGAAGAAAGA
    AGTTCTCAGT CCAAGGCAAC CAAAGGGCAA GAGAAGCAGG ATGAGCAAGA GGAACCATTC
    CTCATGGACA GGCTCTCCTA CCTAGATGAA CAGGCAGGGG CTCCCCCACG CCCCATCCTT
    TTGCCATCTC ACAGACCCCA GAGATACGTG GATGGCCGCC AAGCACCACG CCGCCGTCTG
    CTGCCCCCAA CGCCTGCAGG TCGGAAGCCA TCCTTCACCA TCCAGTGTCT GCGGCGCCAG
    GGCAGCTGTG AAGATTTACC CATCCCAGGC ACCTATCATC GTGGACGAAA CTCAGGGCCC
    AGTAGGGCAC AGGGTTCCTG GGCAACCCCT CCTCAGCGGG GCCGACTCCT ATATGCCCCA
    CTGCTATTGG TGGAGGAGGG TGCAGCAGGG GAGGGATACA TCGGCAAATC CAGCAGTCCA
    CTGCGCACCT TCACCTGTCT GCATGTGCCT GGAACCCACT TGGACCCCAG CCATGGCAAA
    AGGGGCAGTG CTGACAGCCT GGTGGAGGCT GTGCTCATCT CTGAGGGCCT GGGCCTCTTT
    GCCCGAGACC CACGCTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC ATGTCGCCTG
    ACCCTGGACG AGATGGACAG TGCTGCCAGT GACCTGCTGG CACAGGGGAC CAGCTCACTG
    TATAGTGACG AAGAGTCCAT CCTCTCCCGT TTTGATGAGG AGGACCTAGG AGACGAGATG
    GCCTGCGTCC ATGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006073716.1
    CDS1..5721
    Translation

    Target ORF information:

    RefSeq Version XM_006073654.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006073654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTCAGCAT CTGAAGGCGG GAAAGGTGAG AGCAACCTTC CACCCCTACA GACCCTTGGA 
    GCAAGCCTGG ACTCTTGGCA GCCCTTCGAC ATCCTCATCT TGCTGACCAT CTTTGCCAAT
    TGCGTGGCCC TGGGAGTCTA CATCCCCTTC CCAGAGGACG ACTCCAACAC TGCCAACCAC
    AACCTGGAGC AGGTGGAATA CGTATTTTTG GTGATTTTCA CTGTGGAGAC GGTGCTCAAG
    ATCGTGGCCT ACGGGCTGGT GCTCCACCCC AGCGCCTACA TCCGCAACGG CTGGAACCTG
    CTCGACTTCA TCATTGTCGT GGTCGGGCTG TTCAGCGTGC TGCTGGAGCA GGGTCCCGGA
    CGCCCAGGGG ACACCCCGCA TATGGGAGGG AAGCCAGGGG GCTTCGATGT GAAGGCATTG
    CGGGCATTTC GGGTGCTGCG GCCACTGAGG CTGGTGTCTG GAGTCCCAAG CCTGCACATA
    GTGCTCAATT CCATCATGAA GGCTCTGGTG CCGCTGTTGC ATATTGCACT TCTTGTGCTC
    TTTGTCATCA TCATTTACGC CATCATTGGA CTGGAGCTGT TCCTCGGACG CATGCACAAA
    ACGTGCTATT TCCTGGGATC TGACATAGAG GCGGAGGAGG ACCCGTCACC CTGTGCGTCC
    TCAGGATCAG GGCGTGCTTG CACACTGAAC CAGACGGAGT GCCGCGGGCG CTGGGCAGGG
    CCCAACGGAG GCATCACCAA CTTTGACAAC TTTTTCTTCG CCATGCTGAC AGTCTTCCAG
    TGCATCACCA TGGAAGGCTG GACCGACGTG CTCTACTGGA TGCAGGATGC CATGGGGTAT
    GAGCTGCCCT GGGTATATTT CGTGAGTCTC GTCATCTTTG GATCCTTCTT CGTCCTCAAC
    CTTGTACTTG GTGTCCTGAG TGGGGAGTTC TCCAAAGAGA GGGAGAAGGC AAAGGCACGA
    GGGGATTTCC AGAAGCTTCG GGAGAAGCAG CAGCTGGAGG AGGACCTCCG GGGCTACCTG
    GACTGGATCA CACAGGCGGA GGAGCTGGAC ATGGAGGACC CCTCAGCTGC TGGCAACTTT
    GGTTCCGTGG CTGAAGAGGG CCTGGCCGGC CACCGGCCAC AACTGGAAGA GCTGACCAAT
    AGGAGACGAG GACGTCTGCG CTGGTTCAGT CACTCCACCC ACTCCACCAG CAGCCATGCC
    AGCCTCCCAG CCAGTGACAC CGGTTCAATG GCAGAGACCC AAGGAGACGA GGAAGAGGAA
    GAGGGCACTC TAGCCAGCTG TACACGCTGC CTAAATAAGA TCATGAAAAC CAGCGTTTGC
    CGCCGTCTCC GCCGAGCCAA CCGGGGCCTT CGGGCGAGCT GCCGGAGGGC CGTGAAGTCC
    AATGCCTGCT ACTGGGCCGT GTTGCTGCTC GTCTTCCTCA ACACTCTGAC AATCGCCTCG
    GAGCACCACG GGCAGCCCTT CTGGCTCACC CAGATCCAGG AGTATGCCAA CAAAGTGCTA
    CTCTGTCTGT TCACGGTGGA GATGCTTCTC AAGTTGTATG GTCTGGGGCC CTCTGTCTAC
    GTGTCCTCCT TCTTCAACCG CTTTGACTGC TTCGTGGTCT GTGGGGGCAT CCTGGAGACT
    ACCCTGGTGG AGGTGGGTGC CATGCAGCCC CTGGGCATCT CGGTACTCCG CTGTGTGCGC
    CTCCTCAGGA TCTTTAAGGT CACCAGGCAC TGGGCATCTC TGAGCAATTT AGTGGCATCT
    CTACTCAATT CAATGAAATC CATTGCATCC TTGCTGCTTC TCCTCTTCCT CTTTATCATC
    ATCTTCTCCC TGCTTGGCAT GCAGCTGTTT GGGGGCAAGT TCAACTTTGA CCAGACCCAC
    ACCAAGCGAA GCACCTTTGA CACCTTTCCC CAGGCCCTCC TCACTGTCTT TCAGATACTG
    ACGGGTGAGG ACTGGAATGT GGTCATGTAT GATGGTATCA TGGCCTATGG CGGCCCCTTC
    TTCCCAGGGA TGCTGGTGTG CGTGTATTTC ATCATCCTCT TCATCTGTGG CAATTACATC
    CTATTGAATG TGTTTCTTGC CATCGCTGTG GACAACCTGG CCAGTGGAGA TGCAGGCACT
    GACAAAGATA AGGGCAGGGA GAAGATCACT GAGGAAACTC CACAGGAGAA TGGAGCACTG
    GTGCCTGGTG GGGAGAATGA GGAAGAGGAA TCTGCAAAAA ATGAAGGAGC AGACATGGAG
    GAAGAGGAGG AGGAGGAGGA AGAGGAGGAA GAGGAAGTGG GGGCCGGGCA TGTGGAACTC
    CTGCAGGAAG TTGTACCCAA GGAGAAGGTG GTGCCCATTC CTGAGGGCAG TGCTTTCTTC
    TGCCTCAGCC AAACCAACCC GCTGAGGAAG GCCTGCCACA CCCTCATCCA CCATCACATC
    TTCACCAATC TCATCCTGGT GTTTATCATC CTCAGCAGTG TGTCCCTGGC CGCTGAGGAT
    CCCATCCGAG CCCACTCCTT CCGCAACCAC ATTCTGGGGT ACTTCGATTA TGCCTTCACC
    TCCATTTTCA CTGTGGAGAT TCTACTAAAG ATGACAGTGT TCGGGGCTTT TCTGCACCGT
    GGTTCCTTCT GCCGTAGCTG GTTCAATCTG CTGGATCTGC TGGTGGTCAG CGTGTCCCTC
    ATCTCCTTTG GCATCCACTC CAGCGCCATC TCAGTGGTGA AGATTCTGCG TGTACTCCGA
    GTACTGCGGC CCCTTCGAGC TATCAACAGG GCCAAAGGAC TCAAGCACGT GGTGCAATGT
    GTGTTCGTGG CCATCCGGAC CATCGGAAAT ATCATGATCG TGACCACGCT CCTGCAGTTC
    ATGTTCGCCT GCATTGGTGT GCAGCTCTTC AAGGGGAAAT TCTACAGTTG CACTGATGAG
    GCCAAACACA CACCCCAAGA ATGCAAGGGC TCCTTCCTGG TCTACCCCGA TGGAGATGTG
    TCAAGGCCTC TGGTCCGGGA GCGGCTCTGG GTCAACAGCG ATTTCAACTT TGACAATGTC
    CTTTCAGCCA TGATGGCCCT GTTCACTGTC TCCACCTTCG AAGGCTGGCC TGCGCTGCTA
    TACAAGGCCA TTGATGCAAA CGCAGAGGAC AAGGGCCCCA TCTACAATTA CCACGTGGAG
    ATCTCAGTGT TCTTCATTGT CTACATCATC ATCATTGCAT TCTTCATGAT GAACATCTTT
    GTGGGCTTTG TCATCATCAC CTTCCGAGCC CAGGGCGAGC AGGAGTACCA AGACTGTGAG
    CTGGATAAGA ACCAGCGCCA GTGTGTAGAA TACGCCCTCA AGGCCCAACC ACTCCGTCGC
    TATATCCCCA AGAACCCACA TCAGTATCGT GTGTGGGCCA CTGTGAACTC TGCTGCCTTC
    GAGTATCTCA TGTTCCTGCT CATCCTGCTC AACACGGTTG CTCTAGCCAT GCAGCACTAT
    GAGCAGACTG CTCCCTTCAA CTATGCCATG GACATCCTCA ACATGGTCTT CACTGGCCTC
    TTCACTGTTG AGATGGTGCT CAAAATCATT GCCTTCAAAC CCAAGCATTA CTTTACTGAT
    GCCTGGAACA CATTTGATGC TCTTATTGTG GTGGGCAGCG TAGTGGACAT TGCCGTCACT
    GAAGTCAATA ATGGTGGCCA CCTGGGCGAG AGCTCTGAGG ATAGTTCCCG CATTTCGATC
    ACCTTCTTTC GCCTCTTCCG AGTCATGCGA TTGGTCAAGC TTCTTAGTAA GGGTGAAGGG
    ATTCGCACAT TACTTTGGAC ATTTATCAAG TCTTTTCAGG CCTTACCCTA TGTGGCTCTT
    CTCATCGCAA TGATATTCTT CATTTACGCA GTCATTGGGA TGCAGATGTT TGGCAAGGTG
    GCTCTTCAGG ATGGCACACA GATCAACCGA AACAACAACT TCCAGACCTT TCCACAGGCT
    GTACTGCTTT TGTTCAGGTG TGCTACTGGT GAGGCATGGC AGGAGATAAT GCTTGCCAGC
    CTTCCTGGAA GCCGGTGTGA CCCTGAGTCT GACTTCGGCC CTGGTGAGGA GTTTAGCTGT
    GGTAGCAATT TTGCCATCGC CTATTTTATC AGCTTCTTCA TGCTCTGTGC ATTCCTGATC
    ATAAATCTCT TTGTGGCTGT GATCATGGAC AACTTTGATT ATCTAACCAG AGATTGGTCC
    ATCCTGGGCC CCCATCACCT CGATGAATTC AAGAGGATTT GGTCTGAATA TGACCCTGGG
    GCCAAAGGCC GCATCAAGCA CCTGGATGTG GTTGCCCTGC TGAGACGCAT TCAGCCCCCC
    CTGGGATTCG GGAAGCTGTG CCCGCACCGA GTGGCCTGCA AGAGACTTGT GGCGATGAAC
    ATGCCCCTCA ACTCAGATGG GACAGTGACA TTCAATGCCA CACTCTTTGC CCTGGTCCGG
    ACATCACTGA AGATCAAGAC GGAAGGAAAC CTGGAGCAAG CCAATCAGGA GCTGCGGATT
    GTCATCAAAA AGATCTGGAA GCGGATGAAG CAGAAGCTGC TAGATGAGGT CATCCCACCA
    GCTGATGAGG AAGAAGTTAC CGTGGGCAAA TTCTACGCCA CATTTCTGAT CCAGGACTAC
    TTCCGCAAAT TCCGGCGGAG GAAAGAAAAG GGGCTACTAG GCACCGAGGC CCCCCCAAGC
    ACCTCCTCTG CCCTTCAGGC TGGCCTGAGG AGCCTGCAAG ACTTGGGTCC GGAGATGCGG
    CAGGCCCTCA CTTGTGACAC AGAGGAAGAG GAAGGGGAAG AGGGGCAGGA GGGAGAGGAG
    GAACACGAGG ACCTGGAAAC GTACACAGCC CTGATGGGCT CCCAGCCCCC ATCTCGCCGG
    AGCTCCGTGA TTTCTGTGTC TCTACCTGCT GGAGACAAAC CTCCAGATTC ACTTTCCCCT
    GGACCCAGTG ATGACGATGG GGGGGCTCCC AACTCCAGAC AGTCCAATGT TCCCCAGTCT
    GGGTCCCATG CCCACAGGAG AAGCTCTGGG GTTCTCATTT TCACCATTCC AGAAGAAAGA
    AGTTCTCAGT CCAAGGCAAC CAAAGGGCAA GAGAAGCAGG ATGAGCAAGA GGAACCATTC
    CTCATGGACA GGCTCTCCTA CCTAGATGAA CAGGCAGGGG CTCCCCCACG CCCCATCCTT
    TTGCCATCTC ACAGACCCCA GAGATACGTG GATGGCCGCC AAGCACCACG CCGCCGTCTG
    CTGCCCCCAA CGCCTGCAGG TCGGAAGCCA TCCTTCACCA TCCAGTGTCT GCGGCGCCAG
    GGCAGCTGTG AAGATTTACC CATCCCAGGC ACCTATCATC GTGGACGAAA CTCAGGGCCC
    AGTAGGGCAC AGGGTTCCTG GGCAACCCCT CCTCAGCGGG GCCGACTCCT ATATGCCCCA
    CTGCTATTGG TGGAGGAGGG TGCAGCAGGG GAGGGATACA TCGGCAAATC CAGCAGTCCA
    CTGCGCACCT TCACCTGTCT GCATGTGCCT GGAACCCACT TGGACCCCAG CCATGGCAAA
    AGGGGCAGTG CTGACAGCCT GGTGGAGGCT GTGCTCATCT CTGAGGGCCT GGGCCTCTTT
    GCCCGAGACC CACGCTTCGT GGCCCTGGCC AAGCAGGAGA TTGCAGATGC ATGTCGCCTG
    ACCCTGGACG AGATGGACAG TGCTGCCAGT GACCTGCTGG CACAGGGGAC CAGCTCACTG
    TATAGTGACG AAGAGTCCAT CCTCTCCCGT TTTGATGAGG AGGACCTAGG AGACGAGATG
    GCCTGCGTCC ATGCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.