TRPC4 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following TRPC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu21420 XM_006065971.2
Latest version!
Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_006065974.1
Latest version!
Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_006065972.1
Latest version!
Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_025262927.1
Latest version!
Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_025262928.1
Latest version!
Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_006065973.2
Latest version!
Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu47580 XM_025262929.1
Latest version!
Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBu21420 XM_006065971.1 Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu21420 XM_006065973.1 Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785187.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066034.1
    CDS930..3875
    Translation

    Target ORF information:

    RefSeq Version XM_006065972.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_025262927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025118712.1
    CDS115..3060
    Translation

    Target ORF information:

    RefSeq Version XM_025262927.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025262927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065971.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785187.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066033.1
    CDS793..3738
    Translation

    Target ORF information:

    RefSeq Version XM_006065971.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065973.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785187.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066035.1
    CDS277..3222
    Translation

    Target ORF information:

    RefSeq Version XM_006065973.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_025262928.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025118713.1
    CDS848..3793
    Translation

    Target ORF information:

    RefSeq Version XM_025262928.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025262928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006065971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066033.1
    CDS243..3188
    Translation

    Target ORF information:

    RefSeq Version XM_006065971.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065974.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785187.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066036.1
    CDS58..3003
    Translation

    Target ORF information:

    RefSeq Version XM_006065974.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu21420
    Clone ID Related Accession (Same CDS sequence) XM_006065972.1 , XM_025262927.1 , XM_006065971.1 , XM_006065973.1 , XM_025262928.1 , XM_006065971.2 , XM_006065974.1 , XM_006065973.2
    Accession Version XM_006065973.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006065973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006066035.1
    CDS494..3439
    Translation

    Target ORF information:

    RefSeq Version XM_006065973.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065973.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTGTCACCCT CCGAGAAAGC TTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGTTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CTAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTGCTG
    GAAGAACAAA GTGGAAACGA TCTTGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGATGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAAATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGCTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTT CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCG CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCTGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTGAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GTTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACG AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAGGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CCGAAAGACA CACCATCAGC
    AACGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACTGATA TCAGACACTT TGGGTTGTTT CACAGACGAT CGAAACAACA CGTCGCCGCG
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCTCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAGCTC TAGCTTCACG GGGTGACTTG
    AACATCCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTGGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GTAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu47580
    Clone ID Related Accession (Same CDS sequence) XM_025262929.1
    Accession Version XM_025262929.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product short transient receptor potential channel 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGGATG GTGGACTTCA AGATTACATC CATGATTGGT GGAATTTAAT GGATTTTGTG 
    ATGAACTCCT TATACCTAGC AACAATCTCC TTGAAAATCG TTGCATTTGT AAAGTACAGT
    GCTCTTAATC CACGTGAATC TTGGGACATG TGGCACCCCA CGCTGGTGGC TGAAGCTTTG
    TTTGCTATTG CAAATATATT CAGTTCCCTG CGTCTGATCT CACTGTTCAC TGCAAATTCT
    CACCTGGGGC CTCTGCAAAT ATCTCTGGGA AGAATGCTCC TGGACATCTT GAAGTTTCTG
    TTCATCTACT GCCTCGTGCT GCTAGCATTT GCCAATGGCC TAAACCAGCT GTACTTCTAT
    TATGAAGAAA CAAAAGGGTT AAGCTGCAAA GGCATACGAT GTGAAAAGCA GAATAACGCC
    TTTTCAACGT TATTTGAGAC ACTTCAGTCC CTGTTTTGGT CAATATTTGG GCTCATCAAC
    TTATATGTGA CCAATGTCAA AGCGCAGCAT GAATTCACCG AATTTGTTGG TGCCACCATG
    TTTGGGACAT ACAACGTCAT CTCTCTGGTT GTCCTGCTTA ACATGTTAAT AGCTATGATG
    AATAATTCTT ACCAACTTAT TGCTGACCAT GCGGATATAG AATGGAAATT TGCTAGAACA
    AAGCTTTGGA TGAGTTATTT TGAAGAAGGA GGTACCCTTC CTACCCCCTT CAATGTCATC
    CCGAGCCCCA AGTCTCTCTG GTACCTGATC AAATGGATAT GGACACACCT GTGCAAGAAA
    AAGATGAGAA GAAAGCCAGA AAGTTTTGGA ACGATAGGGA GGCGAGCTGC TGATAATTTG
    AGAAGACATC ACCAATATCA AGAAGTTATG CGGAACCTGG TAAAGCGATA CGTTGCTGCA
    ATGATTAGAG ATGCCAAAAC TGAGGAAGGT CTGACAGAGG AGAACTTTAA GGAACTGAAG
    CAAGACATTT CTAGCTTCCG CTTTGAAGTC CTGGGTTTAT TAAGAGGAAG CAAACTCTCC
    ACGGTCCAGT CTGCCCAGGG CACGAAGGAG TCTTCTAACT CCGCAGACTC GGATGAAAAG
    AGTGATAACG AGGGTAGCAG CAAGGACAAG AAAAAGAACT TCAGCCTCTT CGACCTGACC
    ACCCTCATTC ACCCGCGGTC GGCGGCAATT GCCGCCGAAA GACACACCAT CAGCAACGGC
    TCCGCCCTCG TGGTGCAGGA GCCCCCGAGG GAGAAGCAGA GAAAAGTGAA TTTTGTGACT
    GATATCAGAC ACTTTGGGTT GTTTCACAGA CGATCGAAAC AACACGTCGC CGCGGAGCAA
    AACGCAAACC AGATCTTCTC CGTTTCAGAA GGGGTGGCTC GCCAACAAGC TGAAGGACCC
    CTGGAGAGGA GCATCCAGCT GGAGTCCCGA GCTCTAGCTT CACGGGGTGA CTTGAACATC
    CCGGGGCTCA GCGAACAGTG TATATTGGTA GATCATCGAG AAAGAAATAC AGACTCCCTG
    GGTGTACAGG TGAGCAAGAG AGTGTGTTCA TTCAAGTCAG AGAAGGTGGT GGTGGAGGAC
    ACGGTTCCCA TCATACCAAA GGAGAAGAAA CACGCGAAAG AGGAGGACTC GAGTGTAGAC
    TATGATGCAA ACCTCACAGA CACCATCACC CACGAAGACT ATGTGACCAC AAGGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025118714.1
    CDS136..1815
    Translation

    Target ORF information:

    RefSeq Version XM_025262929.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025262929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGGATG GTGGACTTCA AGATTACATC CATGATTGGT GGAATTTAAT GGATTTTGTG 
    ATGAACTCCT TATACCTAGC AACAATCTCC TTGAAAATCG TTGCATTTGT AAAGTACAGT
    GCTCTTAATC CACGTGAATC TTGGGACATG TGGCACCCCA CGCTGGTGGC TGAAGCTTTG
    TTTGCTATTG CAAATATATT CAGTTCCCTG CGTCTGATCT CACTGTTCAC TGCAAATTCT
    CACCTGGGGC CTCTGCAAAT ATCTCTGGGA AGAATGCTCC TGGACATCTT GAAGTTTCTG
    TTCATCTACT GCCTCGTGCT GCTAGCATTT GCCAATGGCC TAAACCAGCT GTACTTCTAT
    TATGAAGAAA CAAAAGGGTT AAGCTGCAAA GGCATACGAT GTGAAAAGCA GAATAACGCC
    TTTTCAACGT TATTTGAGAC ACTTCAGTCC CTGTTTTGGT CAATATTTGG GCTCATCAAC
    TTATATGTGA CCAATGTCAA AGCGCAGCAT GAATTCACCG AATTTGTTGG TGCCACCATG
    TTTGGGACAT ACAACGTCAT CTCTCTGGTT GTCCTGCTTA ACATGTTAAT AGCTATGATG
    AATAATTCTT ACCAACTTAT TGCTGACCAT GCGGATATAG AATGGAAATT TGCTAGAACA
    AAGCTTTGGA TGAGTTATTT TGAAGAAGGA GGTACCCTTC CTACCCCCTT CAATGTCATC
    CCGAGCCCCA AGTCTCTCTG GTACCTGATC AAATGGATAT GGACACACCT GTGCAAGAAA
    AAGATGAGAA GAAAGCCAGA AAGTTTTGGA ACGATAGGGA GGCGAGCTGC TGATAATTTG
    AGAAGACATC ACCAATATCA AGAAGTTATG CGGAACCTGG TAAAGCGATA CGTTGCTGCA
    ATGATTAGAG ATGCCAAAAC TGAGGAAGGT CTGACAGAGG AGAACTTTAA GGAACTGAAG
    CAAGACATTT CTAGCTTCCG CTTTGAAGTC CTGGGTTTAT TAAGAGGAAG CAAACTCTCC
    ACGGTCCAGT CTGCCCAGGG CACGAAGGAG TCTTCTAACT CCGCAGACTC GGATGAAAAG
    AGTGATAACG AGGGTAGCAG CAAGGACAAG AAAAAGAACT TCAGCCTCTT CGACCTGACC
    ACCCTCATTC ACCCGCGGTC GGCGGCAATT GCCGCCGAAA GACACACCAT CAGCAACGGC
    TCCGCCCTCG TGGTGCAGGA GCCCCCGAGG GAGAAGCAGA GAAAAGTGAA TTTTGTGACT
    GATATCAGAC ACTTTGGGTT GTTTCACAGA CGATCGAAAC AACACGTCGC CGCGGAGCAA
    AACGCAAACC AGATCTTCTC CGTTTCAGAA GGGGTGGCTC GCCAACAAGC TGAAGGACCC
    CTGGAGAGGA GCATCCAGCT GGAGTCCCGA GCTCTAGCTT CACGGGGTGA CTTGAACATC
    CCGGGGCTCA GCGAACAGTG TATATTGGTA GATCATCGAG AAAGAAATAC AGACTCCCTG
    GGTGTACAGG TGAGCAAGAG AGTGTGTTCA TTCAAGTCAG AGAAGGTGGT GGTGGAGGAC
    ACGGTTCCCA TCATACCAAA GGAGAAGAAA CACGCGAAAG AGGAGGACTC GAGTGTAGAC
    TATGATGCAA ACCTCACAGA CACCATCACC CACGAAGACT ATGTGACCAC AAGGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.