HCFC1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following HCFC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCFC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu74045 XM_025276875.1
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu74042 XM_025276872.1
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBu74043 XM_025276873.1
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBu74044 XM_025276874.1
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBu02958 XM_006043649.2
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02959 XM_006043650.2
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02960 XM_006043651.2
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02961 XM_006043652.1
Latest version!
Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02962 XM_006043653.1
Latest version!
Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02963 XM_006043654.2
Latest version!
Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBu02963 XM_006043654.1 Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBu02959 XM_006043650.1 Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02958 XM_006043649.1 Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBu02960 XM_006043651.1 Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu74045
    Clone ID Related Accession (Same CDS sequence) XM_025276875.1
    Accession Version XM_025276875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCTT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCC 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCTCT GGAGGTGAGC
    TGGGGGGCCG TGGCGACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CTTCCCTTCC TGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACGGTCA TTGGCAGCAG CCCACAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACAG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACTGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCTGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTAA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCCACA
    GGCCCAGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACGCA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCAGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCTGGAAC TGGAGCCCCT
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACT GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC GCCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATTA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTCA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACAGTCA CCATCGCTGA TTCAGGCCAG GGTGATGTGC AGCCTGGCAC CGTGACCCTG
    GTTTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTC
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCC
    ACCACTGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC GTGCCAAACC CGTCAGACCA GTGCGACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTTG GCCGGCCCGC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCTGCTCTC TTACAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAGG TGCATCACAC GCACACGACC AACACGGCTA CTGTGGCCCG CTCCGCCATG
    AGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC GGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC
    CAGGTCTGCT CCAACCCTCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CAGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCGCC CTGTGAGACG CACGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACACCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCTT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC GGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC TACCCAGTCC GAAGTAGACC AGTTGTCTCT CCCCCAAGAG
    CTGATGGCCG AGGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCTCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATCCAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCGGCGGTGA CACAGGCAGA GCTGAGCCAC
    TTGTCAGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACGCAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC GCTGGCCCCC GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGGGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCTCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCGGCC ACCCTGACGG AAGTGGCTAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGACGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCGGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ATTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATTGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025132660.1
    CDS83..5323
    Translation

    Target ORF information:

    RefSeq Version XM_025276875.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025276875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTTGGG AGACCATCTT GATGGATACG CTGGAAGACA ATATTCCCCG GGCCCGTGCC 
    GGCCACTGTG CGGTAGCCAT CAATACCCGC CTTTACATTT GGAGTGGGCG TGACGGCTAC
    CGAAAGGCCT GGAATAACCA GGTCTGCTGC AAGGACCTCT GGTACCTGGA AACGGAGAAG
    CCGCCACCTC CAGCCCGGGT ACAGCTGGTG CGAGCCAACA CTAACTCTCT GGAGGTGAGC
    TGGGGGGCCG TGGCGACAGC CGACAGTTAC CTTCTGCAGC TCCAGAAATA TGACATTCCT
    GCCACGGCTG CCACTGCCAC CTCCCCCACA CCCAATCCAG TCCCGTCTGT GCCTGCCAAT
    CCTCCCAAGA GCCCTGCCCC AGCAGCAGCC GCACCTGCCG TGCAGCCGCT GACCCAAGTA
    GGCATCACGC TCCTGCCCCA GGCTGCTGCC GCGCCCCCGA CCACCACCAC CATCCAGGTC
    TTGCCGACGG TGCCTGGCAG CTCAATCTCC GTGCCCGCCG CAGCCAGGAC TCAAGGTGTC
    CCGGCTGTGC TGAAAGTGAC TGGTCCTCAG GCTACCACGG GAACCCCGTT GGTCACCATG
    CGACCTGCCA GCCAGGCTGG GAAAGCCCCC GTGACTGTGA CTTCCCTTCC TGCAGGCGTG
    CGGATGGTTG TGCCTACACA GAGCGCCCAG GGGACGGTCA TTGGCAGCAG CCCACAGATG
    AGTGGCATGG CTGCATTGGC AGCCGCGGCT GCTGCCACCC AGAAGATCCC TCCTTCCTCG
    GCACCCACAG TCCTGAGTGT CCCGGCTGGC ACCACTATCG TCAAAACTGT GGCCGTGACG
    CCGGGCACCA CCACGCTCCC AGCCACTGTG AAGGTGGCCT CCTCGCCAGT CATGGTGAGC
    AACCCAGCCA CTCGCATGCT GAAGACTGCA GCTGCCCAGG TGGGGACTTC CGTCTCTTCT
    GCTGCCAACA CATCTACCCG TCCCATCATC ACCGTACACA AGTCCGGAAC CGTGACAGTG
    GCCCAGCAAG CCCAGGTGGT GACCACAGTG GTGGGTGGGG TTACCAAGAC CATCACCCTG
    GTGAAGAGCC CCATCTCGGT CCCAGGAGGC AGTGCTCTAA TTTCCAATCT GGGCAAAGTA
    ATGTCAGTGG TCCAGACCAA ACCGGTTCAG ACTTCGGCAG TCACAGGCCA GGCATCCACA
    GGCCCAGTGA CTCAGATCAT CCAGACCAAA GGACCCTTGC CAGCCGGGAC CATCCTGAAG
    CTGGTGACGT CTGCAGATGG CAAGCCCACT ACCATCATCA CAACCACGCA GGCCAGCGGG
    GCAGGGACTA AGCCTACCAT CCTGGGCATC AGCAGTGTGT CCCCCAGCAC CACCAAGCCC
    GGCACCACCA CTATCATTAA GACCATCCCC ATGTCAGCCA TCATCACACA GGCGGGCGCC
    ACGGGTAGGG GCCTTCCCCG GTGTGCTGGG GAGAAAGCAG GCGTGACTAG CAGTGCCGGC
    ATCAAGTCAC CCATCACCAT TATCACCACC AAGGTGATGA CTTCTGGAAC TGGAGCCCCT
    GCCAAAATCA TCACGGCTGT TCCCAAAATC GCTACGGGTC ACGGGCAGCA AGGAGTGACC
    CAGGTGGTGC TGAAGGGCGC ACCTGGCCAG CCAGGCACCA TCCTCCGCAC CGTGCCTATG
    GGGGGCGTCC GCCTGGTCAC CCCTGTTACT GTCTCTGCTG TCAAGCCTGC TGTCACCACA
    CTGGTTGTCA AGGGCACCAC AGGCGTCACG ACCCTGGGCA CGGTGACAGG CACCGTCTCC
    ACCAGCCTGG CCGGAGCCGG GGGCCATAGC ACCAGCGCCT CACTGGCCAC GCCCATCACC
    ACGTTGGGCA CCATCGCCAC CCTCTCAAGC CAGGTGATTA ACCCCACTGC CATCACTGTG
    TCTGCGGCGC AGACGACGCT GACAGCGGCC GCTGGGCTCA CCACACCCAC CATAACCATG
    CAGCCTGTCT CCCAGCCTAC CCAGGTGACT CTGATAACGG CCCCCAGCGG GGTGGAGGCC
    CAGCCTGTGC ACGACCTCCC AGTGTCCATT CTGGCCTCCC CTACTACAGA ACAGCCCACG
    GCCACAGTCA CCATCGCTGA TTCAGGCCAG GGTGATGTGC AGCCTGGCAC CGTGACCCTG
    GTTTGCTCCA ACCCGCCGTG CGAAACCCAC GAGACGGGCA CCACCAATAC AGCCACCACC
    ACCGTCGTCG CTAATCTGGG GGGGCACCCC CAGCCCACCC AAGTGCAGTT TGTCTGCGAC
    AGACAGGAGG CCACTGCTGC TCTCGTGACC TCCACGGTGG GCCAGCCGAA TGGCAGTGTC
    GTTCGTGTCT GCTCCAACCC GCCGTGTGAA ACCCACGAGA CCGGCACCAC CAGTACAGCC
    ACCACTGCCA TGTCTGGCAT CGGAGGCGGG CCGCGGCGAG ACATCCGGCT CGCCTGCGCG
    GCCACCACCA TTCCCACCGT GGTCCGGGTC AGCGTGACTG CCGGGGCGTC AGAGGGAGCT
    CAGGTTTCCA TCAAGCCCGC GTGCCAAACC CGTCAGACCA GTGCGACCAG CACCACCATG
    ACTGTGATGG CCACCGGGGC CCCGTGCTTG GCCGGCCCGC TCCTCAGACC AAGCGTGGCC
    CTCGAGGCCG CTGGCCGCGG TGCTGCTCTC TTACAGCTGG GGCCACTGAG TGCCCAAGTC
    AGGCCCGGTG GCGAGGAAAG GTCCCTTGCC GGCCTGGGCC CGCTGGTGTC TGTGGGGCGC
    CAGCTGGAGG TGCATCACAC GCACACGACC AACACGGCTA CTGTGGCCCG CTCCGCCATG
    AGTGCCGGGG AGCCCCACGA GCTGCTGGGG GCCCCCACAC TTGTGTACGA GAGCTCAGCC
    AGCGCCTCTG TGACTGCCGC GGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC
    CAGGTCTGCT CCAACCCTCC GTGTGAGACC CACGAGACGG GCACCACCCA CACACCCACC
    ACGGCCACAT CAGGAGGGGC TGCAGGCCAG CCAGAGGGCG GGCAGCAGCC TCCTGCCAGC
    CGGCCCTGTG AGACGCACCA GACCGCTTCC ACTGGCACGA CCATGTCCGT CAGCTTGGGC
    GCCCTGCTCC CGGATGCCGC CCCCTCCCAC AGGACCCTGG AGTCCAGCCT GGAGGTGGCA
    GTGCCACCCG CAGTCGCCCC TCAGGCCGGT GCTTCGTTGC TCACTCCTTT CCCGACGCAA
    AGGGTGTGCT CCAACCCGCC CTGTGAGACG CACGAGACAG GCACTACGCA CACGGCCACC
    ACTGTCACCT CCAACATGAG TTCCAACCAA GACACCCCAC CGCCTGCCGG CGACCAGGGA
    GAGGTGGAGA GCACCCAGGG CGACAGTGTG AATATCGCCA GTTCCAGTCC CATCACGACA
    ACAGTGTCTT CCACGCTGAC GCGGGCCGTG ACCACCGTGA CACAGTCCAC GCCAGTCCCA
    GGCCCTTCGG TGCCGAAGAT CTCATCTGTG ACTGAGACTG CCCCAGGGGC TCTGACCACC
    GAAGTCCCCA TCCCGGCCAC GATTACAGTG ACCATAGCCA ACACAGAAAC TTCTGACATG
    CCCTTCTCTG CTGTTGACAT CCTGCAGCCC CCAGAGGAGC TCCAGGCCTC TCCAGGGCCA
    CGCCAGCAGC TTCCACCACG GCAGCTCCTG CAGCCTGCCT CCGCGCCCTT GGTGGGGGAC
    TCCGCCGAGG TCCTGTCAGC CTCCCAGAGC CCTGAGCTCC AGGCCGCCGT GGATCTGAGC
    AGTACAGGGG ACCCGTCTTC GGGCCAGGAG CCTGCCAGCT CGGCTGTGGT GGCCACCGTG
    GTGGTCCAGC CACCGCCGCC TACCCAGTCC GAAGTAGACC AGTTGTCTCT CCCCCAAGAG
    CTGATGGCCG AGGCCCAGGC GGGCACCACC ACCCTCATGG TGACAGGGCT CACCCCAGAG
    GAGCTGGCAG TGACTGCTGC TGCTGAAGCG GCCGCTCAGG CTGCAGCCAC AGAGGAGGCC
    CAGGCCCTGG CCATCCAGGC CGTGCTCCAG GCCGCACAGC AGGCTGTCAT GGCAGGCACC
    GGGGAGCCCA TGGATACTTC AGAGGCGGCC GCGGCGGTGA CACAGGCAGA GCTGAGCCAC
    TTGTCAGCCG AGGGCCAGGA AGGCCAGGCG ACCACTATCC CCATCGTGCT GACGCAGCAG
    GAGCTGGCTG CCCTGGTCCA GCAGCAGCAG CTCCAGGAAG CACAGGCCCA GCAGCAGCAG
    CACCACCTGC CCACGGAAGC GCTGGCCCCC GCTGACAGCC TCAATGACCC GACCATCGAG
    AGCAACTGCC TCAATGAGCT GGCCGGGGCT GTGCCCAGCA CCGTGGGCCT GCTGCCCCCC
    ACGGCCACTG AGAGCCTGGC CCCGTCCAAC ACATTTGTGG CCCCTCAGCC GGTCGTTGTG
    GCCAGCCCCG CGAAGCTGCA GGCCGCGGCC ACCCTGACGG AAGTGGCTAA TGGCATTGAG
    TCCCTGGGTG TGAAGCCAGA CCTGCCACCG CCACCTAGCA AAGCCCCTGT AAAGAAGGAG
    AACCAGTGGT TTGACGTGGG GGTCATTAAG GGTACCAATG TGATGGTGAC ACACTATTTC
    CTGCCACCCG AAGACGCTGT CCCGACTGAT GATGACTCGG GCACCGTGCC CGACTACAAC
    CAGCTAAAGA AGCAGGAGCT GCAGCCAGGC ACTGCCTATA AGTTCCGCGT CGCCGGGATC
    AATGCCTGCG GCCGGGGGCC CTTCAGTGAG ATCTCAGCCT TTAAAACGTG TCTGCCTGGC
    TTCCCGGGGG CCCCGTGTGC CATTAAAATC AGCAAAAGTC CAGACGGTGC TCACCTCACC
    TGGGAGCCAC CCTCTGTGAC CTCCGGCAAG ATCATCGAGT ATTCAGTGTA CCTGGCCATC
    CAGAGCGCGC AGGCCGGTGG TGAGACCAAG AGCTCGACCC CGGCGCAGCT GGCCTTCATG
    CGGGTGTACT GCGGGCCCAG CCCCTCCTGC CTCGTGCAGT CCTCCAGCCT CTCCAACGCC
    CACATTGACT ACACCACCAA GCCCGCCATC ATCTTCCGCA TTGCTGCCCG CAATGAGAAG
    GGCTACGGCC CAGCCACCCA AGTCAGGTGG TTGCAAGAAA CCAGTAAAGA CAGCTCTGGC
    GCCAAGCCGG CCAGCAAGCG GCCCATGTCG TCTCCAGAAA TGAAATCCGC GCCAAAGAAA
    TCGAAGGCAG ACGGTCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu74042
    Clone ID Related Accession (Same CDS sequence) XM_025276872.1
    Accession Version XM_025276872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCGG CGGTGACACA GGCAGAGCTG
    AGCCACTTGT CAGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACG
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCGCTG GCCCCCGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC TCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACGGAAGT GGCTAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025132657.1
    CDS1555..7701
    Translation

    Target ORF information:

    RefSeq Version XM_025276872.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025276872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCGG CGGTGACACA GGCAGAGCTG
    AGCCACTTGT CAGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACG
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCGCTG GCCCCCGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC TCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACGGAAGT GGCTAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu74043
    Clone ID Related Accession (Same CDS sequence) XM_025276873.1
    Accession Version XM_025276873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG
    ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT
    GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA
    GGGCCACGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCTTGGTG
    GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGCCCTG AGCTCCAGGC CGCCGTGGAT
    CTGAGCAGTA CAGGGGACCC GTCTTCGGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC
    ACCGTGGTGG TCCAGCCACC GCCGCCTACC CAGTCCGAAG TAGACCAGTT GTCTCTCCCC
    CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC
    CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCGGCCG CTCAGGCTGC AGCCACAGAG
    GAGGCCCAGG CCCTGGCCAT CCAGGCCGTG CTCCAGGCCG CACAGCAGGC TGTCATGGCA
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCGG CGGTGACACA GGCAGAGCTG
    AGCCACTTGT CAGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACG
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCGCTG GCCCCCGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC TCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACGGAAGT GGCTAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025132658.1
    CDS1556..7702
    Translation

    Target ORF information:

    RefSeq Version XM_025276873.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025276873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGATCTCA TCTGTGACTG AGACTGCCCC AGGGGCTCTG
    ACCACCGAAG TCCCCATCCC GGCCACGATT ACAGTGACCA TAGCCAACAC AGAAACTTCT
    GACATGCCCT TCTCTGCTGT TGACATCCTG CAGCCCCCAG AGGAGCTCCA GGCCTCTCCA
    GGGCCACGCC AGCAGCTTCC ACCACGGCAG CTCCTGCAGC CTGCCTCCGC GCCCTTGGTG
    GGGGACTCCG CCGAGGTCCT GTCAGCCTCC CAGAGCCCTG AGCTCCAGGC CGCCGTGGAT
    CTGAGCAGTA CAGGGGACCC GTCTTCGGGC CAGGAGCCTG CCAGCTCGGC TGTGGTGGCC
    ACCGTGGTGG TCCAGCCACC GCCGCCTACC CAGTCCGAAG TAGACCAGTT GTCTCTCCCC
    CAAGAGCTGA TGGCCGAGGC CCAGGCGGGC ACCACCACCC TCATGGTGAC AGGGCTCACC
    CCAGAGGAGC TGGCAGTGAC TGCTGCTGCT GAAGCGGCCG CTCAGGCTGC AGCCACAGAG
    GAGGCCCAGG CCCTGGCCAT CCAGGCCGTG CTCCAGGCCG CACAGCAGGC TGTCATGGCA
    GGCACCGGGG AGCCCATGGA TACTTCAGAG GCGGCCGCGG CGGTGACACA GGCAGAGCTG
    AGCCACTTGT CAGCCGAGGG CCAGGAAGGC CAGGCGACCA CTATCCCCAT CGTGCTGACG
    CAGCAGGAGC TGGCTGCCCT GGTCCAGCAG CAGCAGCTCC AGGAAGCACA GGCCCAGCAG
    CAGCAGCACC ACCTGCCCAC GGAAGCGCTG GCCCCCGCTG ACAGCCTCAA TGACCCGACC
    ATCGAGAGCA ACTGCCTCAA TGAGCTGGCC GGGGCTGTGC CCAGCACCGT GGGCCTGCTG
    CCCCCCACGG CCACTGAGAG CCTGGCCCCG TCCAACACAT TTGTGGCCCC TCAGCCGGTC
    GTTGTGGCCA GCCCCGCGAA GCTGCAGGCC GCGGCCACCC TGACGGAAGT GGCTAATGGC
    ATTGAGTCCC TGGGTGTGAA GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu74044
    Clone ID Related Accession (Same CDS sequence) XM_025276874.1
    Accession Version XM_025276874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025132659.1
    CDS1557..7703
    Translation

    Target ORF information:

    RefSeq Version XM_025276874.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025276874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GCCAGACCTG CCACCGCCAC CTAGCAAAGC CCCTGTAAAG
    AAGGAGAACC AGTGGTTTGA CGTGGGGGTC ATTAAGGGTA CCAATGTGAT GGTGACACAC
    TATTTCCTGC CACCCGAAGA CGCTGTCCCG ACTGATGATG ACTCGGGCAC CGTGCCCGAC
    TACAACCAGC TAAAGAAGCA GGAGCTGCAG CCAGGCACTG CCTATAAGTT CCGCGTCGCC
    GGGATCAATG CCTGCGGCCG GGGGCCCTTC AGTGAGATCT CAGCCTTTAA AACGTGTCTG
    CCTGGCTTCC CGGGGGCCCC GTGTGCCATT AAAATCAGCA AAAGTCCAGA CGGTGCTCAC
    CTCACCTGGG AGCCACCCTC TGTGACCTCC GGCAAGATCA TCGAGTATTC AGTGTACCTG
    GCCATCCAGA GCGCGCAGGC CGGTGGTGAG ACCAAGAGCT CGACCCCGGC GCAGCTGGCC
    TTCATGCGGG TGTACTGCGG GCCCAGCCCC TCCTGCCTCG TGCAGTCCTC CAGCCTCTCC
    AACGCCCACA TTGACTACAC CACCAAGCCC GCCATCATCT TCCGCATTGC TGCCCGCAAT
    GAGAAGGGCT ACGGCCCAGC CACCCAAGTC AGGTGGTTGC AAGAAACCAG TAAAGACAGC
    TCTGGCGCCA AGCCGGCCAG CAAGCGGCCC ATGTCGTCTC CAGAAATGAA ATCCGCGCCA
    AAGAAATCGA AGGCAGACGG TCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02958
    Clone ID Related Accession (Same CDS sequence) XM_006043649.1 , XM_006043649.2
    Accession Version XM_006043649.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA TTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATTGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043711.1
    CDS1053..7202
    Translation

    Target ORF information:

    RefSeq Version XM_006043649.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA TTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATTGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02958
    Clone ID Related Accession (Same CDS sequence) XM_006043649.1 , XM_006043649.2
    Accession Version XM_006043649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA TTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATTGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043711.1
    CDS1555..7704
    Translation

    Target ORF information:

    RefSeq Version XM_006043649.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGAAGATC TCATCTGTGA CTGAGACTGC CCCAGGGGCT
    CTGACCACCG AAGTCCCCAT CCCGGCCACG ATTACAGTGA CCATAGCCAA CACAGAAACT
    TCTGACATGC CCTTCTCTGC TGTTGACATC CTGCAGCCCC CAGAGGAGCT CCAGGCCTCT
    CCAGGGCCAC GCCAGCAGCT TCCACCACGG CAGCTCCTGC AGCCTGCCTC CGCGCCCTTG
    GTGGGGGACT CCGCCGAGGT CCTGTCAGCC TCCCAGAGCC CTGAGCTCCA GGCCGCCGTG
    GATCTGAGCA GTACAGGGGA CCCGTCTTCG GGCCAGGAGC CTGCCAGCTC GGCTGTGGTG
    GCCACCGTGG TGGTCCAGCC ACCGCCGCCT ACCCAGTCCG AAGTAGACCA GTTGTCTCTC
    CCCCAAGAGC TGATGGCCGA GGCCCAGGCG GGCACCACCA CCCTCATGGT GACAGGGCTC
    ACCCCAGAGG AGCTGGCAGT GACTGCTGCT GCTGAAGCGG CCGCTCAGGC TGCAGCCACA
    GAGGAGGCCC AGGCCCTGGC CATCCAGGCC GTGCTCCAGG CCGCACAGCA GGCTGTCATG
    GCAGGCACCG GGGAGCCCAT GGATACTTCA GAGGCGGCCG CGGCGGTGAC ACAGGCAGAG
    CTGAGCCACT TGTCAGCCGA GGGCCAGGAA GGCCAGGCGA CCACTATCCC CATCGTGCTG
    ACGCAGCAGG AGCTGGCTGC CCTGGTCCAG CAGCAGCAGC TCCAGGAAGC ACAGGCCCAG
    CAGCAGCAGC ACCACCTGCC CACGGAAGCG CTGGCCCCCG CTGACAGCCT CAATGACCCG
    ACCATCGAGA GCAACTGCCT CAATGAGCTG GCCGGGGCTG TGCCCAGCAC CGTGGGCCTG
    CTGCCCCCCA CGGCCACTGA GAGCCTGGCC CCGTCCAACA CATTTGTGGC CCCTCAGCCG
    GTCGTTGTGG CCAGCCCCGC GAAGCTGCAG GCCGCGGCCA CCCTGACGGA AGTGGCTAAT
    GGCATTGAGT CCCTGGGTGT GAAGCCAGAC CTGCCACCGC CACCTAGCAA AGCCCCTGTA
    AAGAAGGAGA ACCAGTGGTT TGACGTGGGG GTCATTAAGG GTACCAATGT GATGGTGACA
    CACTATTTCC TGCCACCCGA AGACGCTGTC CCGACTGATG ATGACTCGGG CACCGTGCCC
    GACTACAACC AGCTAAAGAA GCAGGAGCTG CAGCCAGGCA CTGCCTATAA GTTCCGCGTC
    GCCGGGATCA ATGCCTGCGG CCGGGGGCCC TTCAGTGAGA TCTCAGCCTT TAAAACGTGT
    CTGCCTGGCT TCCCGGGGGC CCCGTGTGCC ATTAAAATCA GCAAAAGTCC AGACGGTGCT
    CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TCATCGAGTA TTCAGTGTAC
    CTGGCCATCC AGAGCGCGCA GGCCGGTGGT GAGACCAAGA GCTCGACCCC GGCGCAGCTG
    GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TCGTGCAGTC CTCCAGCCTC
    TCCAACGCCC ACATTGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT TGCTGCCCGC
    AATGAGAAGG GCTACGGCCC AGCCACCCAA GTCAGGTGGT TGCAAGAAAC CAGTAAAGAC
    AGCTCTGGCG CCAAGCCGGC CAGCAAGCGG CCCATGTCGT CTCCAGAAAT GAAATCCGCG
    CCAAAGAAAT CGAAGGCAGA CGGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02959
    Clone ID Related Accession (Same CDS sequence) XM_006043650.2 , XM_006043650.1
    Accession Version XM_006043650.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCGGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCTACCCAG
    TCCGAAGTAG ACCAGTTGTC TCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCTC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCGGCGG TGACACAGGC AGAGCTGAGC CACTTGTCAG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACGCAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCGCTGGCC
    CCCGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGGG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCTCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG
    GCCACCCTGA CGGAAGTGGC TAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTATTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATTG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043712.1
    CDS1554..7667
    Translation

    Target ORF information:

    RefSeq Version XM_006043650.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043650.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCGGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCTACCCAG
    TCCGAAGTAG ACCAGTTGTC TCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCTC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCGGCGG TGACACAGGC AGAGCTGAGC CACTTGTCAG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACGCAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCGCTGGCC
    CCCGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGGG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCTCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG
    GCCACCCTGA CGGAAGTGGC TAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTATTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATTG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02959
    Clone ID Related Accession (Same CDS sequence) XM_006043650.2 , XM_006043650.1
    Accession Version XM_006043650.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCGGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCTACCCAG
    TCCGAAGTAG ACCAGTTGTC TCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCTC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCGGCGG TGACACAGGC AGAGCTGAGC CACTTGTCAG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACGCAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCGCTGGCC
    CCCGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGGG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCTCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG
    GCCACCCTGA CGGAAGTGGC TAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTATTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATTG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043712.1
    CDS1053..7166
    Translation

    Target ORF information:

    RefSeq Version XM_006043650.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGAA GATCTCATCT
    GTGACTGAGA CTGCCCCAGG GGCTCTGACC ACCGAAGTCC CCATCCCGGC CACGATTACA
    GTGACCATAG CCAACACAGA AACTTCTGAC ATGCCCTTCT CTGCTGTTGA CATCCTGCAG
    CCCCCAGAGG AGCTCCAGGC CTCTCCAGGG CCACGCCAGC AGCTTCCACC ACGGCAGCTC
    CTGCAGCCTG CCTCCGCGCC CTTGGTGGGG GACTCCGCCG AGGTCCTGTC AGCCTCCCAG
    AGCCCTGAGC TCCAGGCCGC CGTGGATCTG AGCAGTACAG GGGACCCGTC TTCGGGCCAG
    GAGCCTGCCA GCTCGGCTGT GGTGGCCACC GTGGTGGTCC AGCCACCGCC GCCTACCCAG
    TCCGAAGTAG ACCAGTTGTC TCTCCCCCAA GAGCTGATGG CCGAGGCCCA GGCGGGCACC
    ACCACCCTCA TGGTGACAGG GCTCACCCCA GAGGAGCTGG CAGTGACTGC TGCTGCTGAA
    GCGGCCGCTC AGGCTGCAGC CACAGAGGAG GCCCAGGCCC TGGCCATCCA GGCCGTGCTC
    CAGGCCGCAC AGCAGGCTGT CATGGCAGGC ACCGGGGAGC CCATGGATAC TTCAGAGGCG
    GCCGCGGCGG TGACACAGGC AGAGCTGAGC CACTTGTCAG CCGAGGGCCA GGAAGGCCAG
    GCGACCACTA TCCCCATCGT GCTGACGCAG CAGGAGCTGG CTGCCCTGGT CCAGCAGCAG
    CAGCTCCAGG AAGCACAGGC CCAGCAGCAG CAGCACCACC TGCCCACGGA AGCGCTGGCC
    CCCGCTGACA GCCTCAATGA CCCGACCATC GAGAGCAACT GCCTCAATGA GCTGGCCGGG
    GCTGTGCCCA GCACCGTGGG CCTGCTGCCC CCCACGGCCA CTGAGAGCCT GGCCCCGTCC
    AACACATTTG TGGCCCCTCA GCCGGTCGTT GTGGCCAGCC CCGCGAAGCT GCAGGCCGCG
    GCCACCCTGA CGGAAGTGGC TAATGGCATT GAGTCCCTGG GTGTGAAGCC AGACCTGCCA
    CCGCCACCTA GCAAAGCCCC TGTAAAGAAG GAGAACCAGT GGTTTGACGT GGGGGTCATT
    AAGGGTACCA ATGTGATGGT GACACACTAT TTCCTGCCAC CCGAAGACGC TGTCCCGACT
    GATGATGACT CGGGCACCGT GCCCGACTAC AACCAGCTAA AGAAGCAGGA GCTGCAGCCA
    GGCACTGCCT ATAAGTTCCG CGTCGCCGGG ATCAATGCCT GCGGCCGGGG GCCCTTCAGT
    GAGATCTCAG CCTTTAAAAC GTGTCTGCCT GGCTTCCCGG GGGCCCCGTG TGCCATTAAA
    ATCAGCAAAA GTCCAGACGG TGCTCACCTC ACCTGGGAGC CACCCTCTGT GACCTCCGGC
    AAGATCATCG AGTATTCAGT GTACCTGGCC ATCCAGAGCG CGCAGGCCGG TGGTGAGACC
    AAGAGCTCGA CCCCGGCGCA GCTGGCCTTC ATGCGGGTGT ACTGCGGGCC CAGCCCCTCC
    TGCCTCGTGC AGTCCTCCAG CCTCTCCAAC GCCCACATTG ACTACACCAC CAAGCCCGCC
    ATCATCTTCC GCATTGCTGC CCGCAATGAG AAGGGCTACG GCCCAGCCAC CCAAGTCAGG
    TGGTTGCAAG AAACCAGTAA AGACAGCTCT GGCGCCAAGC CGGCCAGCAA GCGGCCCATG
    TCGTCTCCAG AAATGAAATC CGCGCCAAAG AAATCGAAGG CAGACGGTCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02960
    Clone ID Related Accession (Same CDS sequence) XM_006043651.1 , XM_006043651.2
    Accession Version XM_006043651.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCGTGCCCGA CTACAACCAG
    CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT
    GCCTGCGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACGTGTCT GCCTGGCTTC
    CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG
    GAGCCACCCT CTGTGACCTC CGGCAAGATC ATCGAGTATT CAGTGTACCT GGCCATCCAG
    AGCGCGCAGG CCGGTGGTGA GACCAAGAGC TCGACCCCGG CGCAGCTGGC CTTCATGCGG
    GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC
    ATTGACTACA CCACCAAGCC CGCCATCATC TTCCGCATTG CTGCCCGCAA TGAGAAGGGC
    TACGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC
    AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG
    AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043713.1
    CDS1053..7070
    Translation

    Target ORF information:

    RefSeq Version XM_006043651.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCGTGCCCGA CTACAACCAG
    CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT
    GCCTGCGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACGTGTCT GCCTGGCTTC
    CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG
    GAGCCACCCT CTGTGACCTC CGGCAAGATC ATCGAGTATT CAGTGTACCT GGCCATCCAG
    AGCGCGCAGG CCGGTGGTGA GACCAAGAGC TCGACCCCGG CGCAGCTGGC CTTCATGCGG
    GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC
    ATTGACTACA CCACCAAGCC CGCCATCATC TTCCGCATTG CTGCCCGCAA TGAGAAGGGC
    TACGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC
    AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG
    AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02960
    Clone ID Related Accession (Same CDS sequence) XM_006043651.1 , XM_006043651.2
    Accession Version XM_006043651.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCGTGCCCGA CTACAACCAG
    CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT
    GCCTGCGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACGTGTCT GCCTGGCTTC
    CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG
    GAGCCACCCT CTGTGACCTC CGGCAAGATC ATCGAGTATT CAGTGTACCT GGCCATCCAG
    AGCGCGCAGG CCGGTGGTGA GACCAAGAGC TCGACCCCGG CGCAGCTGGC CTTCATGCGG
    GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC
    ATTGACTACA CCACCAAGCC CGCCATCATC TTCCGCATTG CTGCCCGCAA TGAGAAGGGC
    TACGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC
    AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG
    AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043713.1
    CDS1555..7572
    Translation

    Target ORF information:

    RefSeq Version XM_006043651.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043651.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGA AGCCAGACCT GCCACCGCCA CCTAGCAAAG CCCCTGTAAA GAAGGAGAAC
    CAGTGGTTTG ACGTGGGGGT CATTAAGGGT ACCAATGTGA TGGTGACACA CTATTTCCTG
    CCACCCGAAG ACGCTGTCCC GACTGATGAT GACTCGGGCA CCGTGCCCGA CTACAACCAG
    CTAAAGAAGC AGGAGCTGCA GCCAGGCACT GCCTATAAGT TCCGCGTCGC CGGGATCAAT
    GCCTGCGGCC GGGGGCCCTT CAGTGAGATC TCAGCCTTTA AAACGTGTCT GCCTGGCTTC
    CCGGGGGCCC CGTGTGCCAT TAAAATCAGC AAAAGTCCAG ACGGTGCTCA CCTCACCTGG
    GAGCCACCCT CTGTGACCTC CGGCAAGATC ATCGAGTATT CAGTGTACCT GGCCATCCAG
    AGCGCGCAGG CCGGTGGTGA GACCAAGAGC TCGACCCCGG CGCAGCTGGC CTTCATGCGG
    GTGTACTGCG GGCCCAGCCC CTCCTGCCTC GTGCAGTCCT CCAGCCTCTC CAACGCCCAC
    ATTGACTACA CCACCAAGCC CGCCATCATC TTCCGCATTG CTGCCCGCAA TGAGAAGGGC
    TACGGCCCAG CCACCCAAGT CAGGTGGTTG CAAGAAACCA GTAAAGACAG CTCTGGCGCC
    AAGCCGGCCA GCAAGCGGCC CATGTCGTCT CCAGAAATGA AATCCGCGCC AAAGAAATCG
    AAGGCAGACG GTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02961
    Clone ID Related Accession (Same CDS sequence) XM_006043652.1
    Accession Version XM_006043652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACCGGGGAG
    CCCATGGATA CTTCAGAGGC GGCCGCGGCG GTGACACAGG CAGAGCTGAG CCACTTGTCA
    GCCGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATCG TGCTGACGCA GCAGGAGCTG
    GCTGCCCTGG TCCAGCAGCA GCAGCTCCAG GAAGCACAGG CCCAGCAGCA GCAGCACCAC
    CTGCCCACGG AAGCGCTGGC CCCCGCTGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC
    TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGG GCCTGCTGCC CCCCACGGCC
    ACTGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCTC AGCCGGTCGT TGTGGCCAGC
    CCCGCGAAGC TGCAGGCCGC GGCCACCCTG ACGGAAGTGG CTAATGGCAT TGAGTCCCTG
    GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCG
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTATTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATT
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043714.1
    CDS1053..7067
    Translation

    Target ORF information:

    RefSeq Version XM_006043652.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGG CACCGGGGAG
    CCCATGGATA CTTCAGAGGC GGCCGCGGCG GTGACACAGG CAGAGCTGAG CCACTTGTCA
    GCCGAGGGCC AGGAAGGCCA GGCGACCACT ATCCCCATCG TGCTGACGCA GCAGGAGCTG
    GCTGCCCTGG TCCAGCAGCA GCAGCTCCAG GAAGCACAGG CCCAGCAGCA GCAGCACCAC
    CTGCCCACGG AAGCGCTGGC CCCCGCTGAC AGCCTCAATG ACCCGACCAT CGAGAGCAAC
    TGCCTCAATG AGCTGGCCGG GGCTGTGCCC AGCACCGTGG GCCTGCTGCC CCCCACGGCC
    ACTGAGAGCC TGGCCCCGTC CAACACATTT GTGGCCCCTC AGCCGGTCGT TGTGGCCAGC
    CCCGCGAAGC TGCAGGCCGC GGCCACCCTG ACGGAAGTGG CTAATGGCAT TGAGTCCCTG
    GGTGTGAAGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCG
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTATTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATT
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02962
    Clone ID Related Accession (Same CDS sequence) XM_006043653.1
    Accession Version XM_006043653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCG
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTATTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATT
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043715.1
    CDS1053..7067
    Translation

    Target ORF information:

    RefSeq Version XM_006043653.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGTAGGGG CCTTCCCCGG TGTGCTGGGG AGAAAGCAGG CGTGACTAGC
    AGTGCCGGCA TCAAGTCACC CATCACCATT ATCACCACCA AGGTGATGAC TTCTGGAACT
    GGAGCCCCTG CCAAAATCAT CACGGCTGTT CCCAAAATCG CTACGGGTCA CGGGCAGCAA
    GGAGTGACCC AGGTGGTGCT GAAGGGCGCA CCTGGCCAGC CAGGCACCAT CCTCCGCACC
    GTGCCTATGG GGGGCGTCCG CCTGGTCACC CCTGTTACTG TCTCTGCTGT CAAGCCTGCT
    GTCACCACAC TGGTTGTCAA GGGCACCACA GGCGTCACGA CCCTGGGCAC GGTGACAGGC
    ACCGTCTCCA CCAGCCTGGC CGGAGCCGGG GGCCATAGCA CCAGCGCCTC ACTGGCCACG
    CCCATCACCA CGTTGGGCAC CATCGCCACC CTCTCAAGCC AGGTGATTAA CCCCACTGCC
    ATCACTGTGT CTGCGGCGCA GACGACGCTG ACAGCGGCCG CTGGGCTCAC CACACCCACC
    ATAACCATGC AGCCTGTCTC CCAGCCTACC CAGGTGACTC TGATAACGGC CCCCAGCGGG
    GTGGAGGCCC AGCCTGTGCA CGACCTCCCA GTGTCCATTC TGGCCTCCCC TACTACAGAA
    CAGCCCACGG CCACAGTCAC CATCGCTGAT TCAGGCCAGG GTGATGTGCA GCCTGGCACC
    GTGACCCTGG TTTGCTCCAA CCCGCCGTGC GAAACCCACG AGACGGGCAC CACCAATACA
    GCCACCACCA CCGTCGTCGC TAATCTGGGG GGGCACCCCC AGCCCACCCA AGTGCAGTTT
    GTCTGCGACA GACAGGAGGC CACTGCTGCT CTCGTGACCT CCACGGTGGG CCAGCCGAAT
    GGCAGTGTCG TTCGTGTCTG CTCCAACCCG CCGTGTGAAA CCCACGAGAC CGGCACCACC
    AGTACAGCCA CCACTGCCAT GTCTGGCATC GGAGGCGGGC CGCGGCGAGA CATCCGGCTC
    GCCTGCGCGG CCACCACCAT TCCCACCGTG GTCCGGGTCA GCGTGACTGC CGGGGCGTCA
    GAGGGAGCTC AGGTTTCCAT CAAGCCCGCG TGCCAAACCC GTCAGACCAG TGCGACCAGC
    ACCACCATGA CTGTGATGGC CACCGGGGCC CCGTGCTTGG CCGGCCCGCT CCTCAGACCA
    AGCGTGGCCC TCGAGGCCGC TGGCCGCGGT GCTGCTCTCT TACAGCTGGG GCCACTGAGT
    GCCCAAGTCA GGCCCGGTGG CGAGGAAAGG TCCCTTGCCG GCCTGGGCCC GCTGGTGTCT
    GTGGGGCGCC AGCTGGAGGT GCATCACACG CACACGACCA ACACGGCTAC TGTGGCCCGC
    TCCGCCATGA GTGCCGGGGA GCCCCACGAG CTGCTGGGGG CCCCCACACT TGTGTACGAG
    AGCTCAGCCA GCGCCTCTGT GACTGCCGCG GCCCTGGAGG CACTGCTGTG CCCCTCGGCC
    ACCGTGACCC AGGTCTGCTC CAACCCTCCG TGTGAGACCC ACGAGACGGG CACCACCCAC
    ACACCCACCA CGGCCACATC AGGAGGGGCT GCAGGCCAGC CAGAGGGCGG GCAGCAGCCT
    CCTGCCAGCC GGCCCTGTGA GACGCACCAG ACCGCTTCCA CTGGCACGAC CATGTCCGTC
    AGCTTGGGCG CCCTGCTCCC GGATGCCGCC CCCTCCCACA GGACCCTGGA GTCCAGCCTG
    GAGGTGGCAG TGCCACCCGC AGTCGCCCCT CAGGCCGGTG CTTCGTTGCT CACTCCTTTC
    CCGACGCAAA GGGTGTGCTC CAACCCGCCC TGTGAGACGC ACGAGACAGG CACTACGCAC
    ACGGCCACCA CTGTCACCTC CAACATGAGT TCCAACCAAG ACACCCCACC GCCTGCCGGC
    GACCAGGGAG AGGTGGAGAG CACCCAGGGC GACAGTGTGA ATATCGCCAG TTCCAGTCCC
    ATCACGACAA CAGTGTCTTC CACGCTGACG CGGGCCGTGA CCACCGTGAC ACAGTCCACG
    CCAGTCCCAG GCCCTTCGGT GCCGCCCCCA GAGGAGCTCC AGGCCTCTCC AGGGCCACGC
    CAGCAGCTTC CACCACGGCA GCTCCTGCAG CCTGCCTCCG CGCCCTTGGT GGGGGACTCC
    GCCGAGGTCC TGTCAGCCTC CCAGAGCCCT GAGCTCCAGG CCGCCGTGGA TCTGAGCAGT
    ACAGGGGACC CGTCTTCGGG CCAGGAGCCT GCCAGCTCGG CTGTGGTGGC CACCGTGGTG
    GTCCAGCCAC CGCCGCCTAC CCAGTCCGAA GTAGACCAGT TGTCTCTCCC CCAAGAGCTG
    ATGGCCGAGG CCCAGGCGGG CACCACCACC CTCATGGTGA CAGGGCTCAC CCCAGAGGAG
    CTGGCAGTGA CTGCTGCTGC TGAAGCGGCC GCTCAGGCTG CAGCCACAGA GGAGGCCCAG
    GCCCTGGCCA TCCAGGCCGT GCTCCAGGCC GCACAGCAGG CTGTCATGGC AGGCACCGGG
    GAGCCCATGG ATACTTCAGA GGCGGCCGCG GCGGTGACAC AGGCAGAGCT GAGCCACTTG
    TCAGCCGAGG GCCAGGAAGG CCAGGCGACC ACTATCCCCA TCGTGCTGAC GCAGCAGGAG
    CTGGCTGCCC TGGTCCAGCA GCAGCAGCTC CAGGAAGCAC AGGCCCAGCA GCAGCAGCAC
    CACCTGCCCA CGGAAGCGCT GGCCCCCGCT GACAGCCTCA ATGACCCGAC CATCGAGAGC
    AACTGCCTCA ATGAGCTGGC CGGGGCTGTG CCCAGCACCG TGGGCCTGCT GCCCCCCACG
    GCCACTGAGA GCCTGGCCCC GTCCAACACA TTTGTGGCCC CTCAGCCGGT CGTTGTGGCC
    AGCCCCGCGA AGCTGCAGGC CGCGGCCACC CTGACGGAAG TGGCTAATGG CATTGAGTCC
    CTGGGTGTGC CAGACCTGCC ACCGCCACCT AGCAAAGCCC CTGTAAAGAA GGAGAACCAG
    TGGTTTGACG TGGGGGTCAT TAAGGGTACC AATGTGATGG TGACACACTA TTTCCTGCCA
    CCCGAAGACG CTGTCCCGAC TGATGATGAC TCGGGCACCG TGCCCGACTA CAACCAGCTA
    AAGAAGCAGG AGCTGCAGCC AGGCACTGCC TATAAGTTCC GCGTCGCCGG GATCAATGCC
    TGCGGCCGGG GGCCCTTCAG TGAGATCTCA GCCTTTAAAA CGTGTCTGCC TGGCTTCCCG
    GGGGCCCCGT GTGCCATTAA AATCAGCAAA AGTCCAGACG GTGCTCACCT CACCTGGGAG
    CCACCCTCTG TGACCTCCGG CAAGATCATC GAGTATTCAG TGTACCTGGC CATCCAGAGC
    GCGCAGGCCG GTGGTGAGAC CAAGAGCTCG ACCCCGGCGC AGCTGGCCTT CATGCGGGTG
    TACTGCGGGC CCAGCCCCTC CTGCCTCGTG CAGTCCTCCA GCCTCTCCAA CGCCCACATT
    GACTACACCA CCAAGCCCGC CATCATCTTC CGCATTGCTG CCCGCAATGA GAAGGGCTAC
    GGCCCAGCCA CCCAAGTCAG GTGGTTGCAA GAAACCAGTA AAGACAGCTC TGGCGCCAAG
    CCGGCCAGCA AGCGGCCCAT GTCGTCTCCA GAAATGAAAT CCGCGCCAAA GAAATCGAAG
    GCAGACGGTC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02963
    Clone ID Related Accession (Same CDS sequence) XM_006043654.1 , XM_006043654.2
    Accession Version XM_006043654.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CGGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CTACCCAGTC CGAAGTAGAC
    CAGTTGTCTC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCTCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC GGCGGTGACA
    CAGGCAGAGC TGAGCCACTT GTCAGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CGCAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCGC TGGCCCCCGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGGGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCTCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACGGAA
    GTGGCTAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAT
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATTGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043716.1
    CDS1053..7031
    Translation

    Target ORF information:

    RefSeq Version XM_006043654.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (VP16-accessory protein) (HCFC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CGGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CTACCCAGTC CGAAGTAGAC
    CAGTTGTCTC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCTCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC GGCGGTGACA
    CAGGCAGAGC TGAGCCACTT GTCAGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CGCAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCGC TGGCCCCCGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGGGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCTCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACGGAA
    GTGGCTAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAT
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATTGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu02963
    Clone ID Related Accession (Same CDS sequence) XM_006043654.1 , XM_006043654.2
    Accession Version XM_006043654.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product host cell factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006043654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CGGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CTACCCAGTC CGAAGTAGAC
    CAGTTGTCTC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCTCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC GGCGGTGACA
    CAGGCAGAGC TGAGCCACTT GTCAGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CGCAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCGC TGGCCCCCGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGGGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCTCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACGGAA
    GTGGCTAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAT
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATTGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006043716.1
    CDS1553..7531
    Translation

    Target ORF information:

    RefSeq Version XM_006043654.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis host cell factor C1 (HCFC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006043654.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTTCGG CCGTGTCACC CGCCAACTCG CCAGCGGTGC TCTTGCAGCC CCGCTGGAAG 
    CGAGTGGTGG GCTGGTCGGG TCCAGTGCCC CGGCCCCGCC ACGGCCACCG CGCCGTGGCC
    ATCAAGGAGC TCATCGTGGT GTTTGGCGGC GGCAACGAGG GGATAGTGGA CGAGCTGCAC
    GTGTACAACA CGGCGACCAA CCAGTGGTTC ATCCCAGCTG TGAGAGGGGA CATCCCTCCT
    GGGTGTGCAG CCTATGGCTT CGTGTGCGAT GGGACACGCC TGCTCGTGTT CGGAGGGATG
    GTGGAATACG GGAAGTACAG CAACGACCTC TACGAGCTCC AGGCAAGCCG GTGGGAATGG
    AAGAGACTGA AAGCAAAGAC GCCCAAAAAT GGGCCACCTC CGTGTCCTCG GCTTGGGCAC
    AGCTTCTCCC TTGTGGGCAA CAAGTGTTAC CTGTTTGGGG GTCTGGCCAA TGATAGCGAG
    GACCCCAAGA ATAACATTCC GAGGTACCTG AATGATTTAT ACATCCTGGA ACTGCGGCCG
    GGCTCTGGAG TGGTAGCCTG GGACATTCCC ATCACTTACG GTGTCCTGCC CCCACCCCGA
    GAGTCTCACA CTGCAGTGGT CTACACTGAG AAAGACAACA AGAAGTCCAA ACTGGTGATC
    TATGGAGGGA TGAGTGGCTG CAGGCTGGGG GACCTCTGGA CCTTGGATAT CGAGACTTTG
    ACATGGAATA AGCCCAGCCT CAGCGGGGTG GCACCTCTTC CTCGAAGTCT CCACTCGGCC
    ACGACCATAG GAAACAAAAT GTACGTGTTT GGTGGCTGGG TGCCTCTCGT CATGGATGAC
    GTCAAAGTGG CCACACACGA GAAGGAGTGG AAGTGTACCA ACACTCTGGC TTGTCTCAAC
    CTGGATACCA TGGCTTGGGA GACCATCTTG ATGGATACGC TGGAAGACAA TATTCCCCGG
    GCCCGTGCCG GCCACTGTGC GGTAGCCATC AATACCCGCC TTTACATTTG GAGTGGGCGT
    GACGGCTACC GAAAGGCCTG GAATAACCAG GTCTGCTGCA AGGACCTCTG GTACCTGGAA
    ACGGAGAAGC CGCCACCTCC AGCCCGGGTA CAGCTGGTGC GAGCCAACAC TAACTCTCTG
    GAGGTGAGCT GGGGGGCCGT GGCGACAGCC GACAGTTACC TTCTGCAGCT CCAGAAATAT
    GACATTCCTG CCACGGCTGC CACTGCCACC TCCCCCACAC CCAATCCAGT CCCGTCTGTG
    CCTGCCAATC CTCCCAAGAG CCCTGCCCCA GCAGCAGCCG CACCTGCCGT GCAGCCGCTG
    ACCCAAGTAG GCATCACGCT CCTGCCCCAG GCTGCTGCCG CGCCCCCGAC CACCACCACC
    ATCCAGGTCT TGCCGACGGT GCCTGGCAGC TCAATCTCCG TGCCCGCCGC AGCCAGGACT
    CAAGGTGTCC CGGCTGTGCT GAAAGTGACT GGTCCTCAGG CTACCACGGG AACCCCGTTG
    GTCACCATGC GACCTGCCAG CCAGGCTGGG AAAGCCCCCG TGACTGTGAC TTCCCTTCCT
    GCAGGCGTGC GGATGGTTGT GCCTACACAG AGCGCCCAGG GGACGGTCAT TGGCAGCAGC
    CCACAGATGA GTGGCATGGC TGCATTGGCA GCCGCGGCTG CTGCCACCCA GAAGATCCCT
    CCTTCCTCGG CACCCACAGT CCTGAGTGTC CCGGCTGGCA CCACTATCGT CAAAACTGTG
    GCCGTGACGC CGGGCACCAC CACGCTCCCA GCCACTGTGA AGGTGGCCTC CTCGCCAGTC
    ATGGTGAGCA ACCCAGCCAC TCGCATGCTG AAGACTGCAG CTGCCCAGGT GGGGACTTCC
    GTCTCTTCTG CTGCCAACAC ATCTACCCGT CCCATCATCA CCGTACACAA GTCCGGAACC
    GTGACAGTGG CCCAGCAAGC CCAGGTGGTG ACCACAGTGG TGGGTGGGGT TACCAAGACC
    ATCACCCTGG TGAAGAGCCC CATCTCGGTC CCAGGAGGCA GTGCTCTAAT TTCCAATCTG
    GGCAAAGTAA TGTCAGTGGT CCAGACCAAA CCGGTTCAGA CTTCGGCAGT CACAGGCCAG
    GCATCCACAG GCCCAGTGAC TCAGATCATC CAGACCAAAG GACCCTTGCC AGCCGGGACC
    ATCCTGAAGC TGGTGACGTC TGCAGATGGC AAGCCCACTA CCATCATCAC AACCACGCAG
    GCCAGCGGGG CAGGGACTAA GCCTACCATC CTGGGCATCA GCAGTGTGTC CCCCAGCACC
    ACCAAGCCCG GCACCACCAC TATCATTAAG ACCATCCCCA TGTCAGCCAT CATCACACAG
    GCGGGCGCCA CGGGCGTGAC TAGCAGTGCC GGCATCAAGT CACCCATCAC CATTATCACC
    ACCAAGGTGA TGACTTCTGG AACTGGAGCC CCTGCCAAAA TCATCACGGC TGTTCCCAAA
    ATCGCTACGG GTCACGGGCA GCAAGGAGTG ACCCAGGTGG TGCTGAAGGG CGCACCTGGC
    CAGCCAGGCA CCATCCTCCG CACCGTGCCT ATGGGGGGCG TCCGCCTGGT CACCCCTGTT
    ACTGTCTCTG CTGTCAAGCC TGCTGTCACC ACACTGGTTG TCAAGGGCAC CACAGGCGTC
    ACGACCCTGG GCACGGTGAC AGGCACCGTC TCCACCAGCC TGGCCGGAGC CGGGGGCCAT
    AGCACCAGCG CCTCACTGGC CACGCCCATC ACCACGTTGG GCACCATCGC CACCCTCTCA
    AGCCAGGTGA TTAACCCCAC TGCCATCACT GTGTCTGCGG CGCAGACGAC GCTGACAGCG
    GCCGCTGGGC TCACCACACC CACCATAACC ATGCAGCCTG TCTCCCAGCC TACCCAGGTG
    ACTCTGATAA CGGCCCCCAG CGGGGTGGAG GCCCAGCCTG TGCACGACCT CCCAGTGTCC
    ATTCTGGCCT CCCCTACTAC AGAACAGCCC ACGGCCACAG TCACCATCGC TGATTCAGGC
    CAGGGTGATG TGCAGCCTGG CACCGTGACC CTGGTTTGCT CCAACCCGCC GTGCGAAACC
    CACGAGACGG GCACCACCAA TACAGCCACC ACCACCGTCG TCGCTAATCT GGGGGGGCAC
    CCCCAGCCCA CCCAAGTGCA GTTTGTCTGC GACAGACAGG AGGCCACTGC TGCTCTCGTG
    ACCTCCACGG TGGGCCAGCC GAATGGCAGT GTCGTTCGTG TCTGCTCCAA CCCGCCGTGT
    GAAACCCACG AGACCGGCAC CACCAGTACA GCCACCACTG CCATGTCTGG CATCGGAGGC
    GGGCCGCGGC GAGACATCCG GCTCGCCTGC GCGGCCACCA CCATTCCCAC CGTGGTCCGG
    GTCAGCGTGA CTGCCGGGGC GTCAGAGGGA GCTCAGGTTT CCATCAAGCC CGCGTGCCAA
    ACCCGTCAGA CCAGTGCGAC CAGCACCACC ATGACTGTGA TGGCCACCGG GGCCCCGTGC
    TTGGCCGGCC CGCTCCTCAG ACCAAGCGTG GCCCTCGAGG CCGCTGGCCG CGGTGCTGCT
    CTCTTACAGC TGGGGCCACT GAGTGCCCAA GTCAGGCCCG GTGGCGAGGA AAGGTCCCTT
    GCCGGCCTGG GCCCGCTGGT GTCTGTGGGG CGCCAGCTGG AGGTGCATCA CACGCACACG
    ACCAACACGG CTACTGTGGC CCGCTCCGCC ATGAGTGCCG GGGAGCCCCA CGAGCTGCTG
    GGGGCCCCCA CACTTGTGTA CGAGAGCTCA GCCAGCGCCT CTGTGACTGC CGCGGCCCTG
    GAGGCACTGC TGTGCCCCTC GGCCACCGTG ACCCAGGTCT GCTCCAACCC TCCGTGTGAG
    ACCCACGAGA CGGGCACCAC CCACACACCC ACCACGGCCA CATCAGGAGG GGCTGCAGGC
    CAGCCAGAGG GCGGGCAGCA GCCTCCTGCC AGCCGGCCCT GTGAGACGCA CCAGACCGCT
    TCCACTGGCA CGACCATGTC CGTCAGCTTG GGCGCCCTGC TCCCGGATGC CGCCCCCTCC
    CACAGGACCC TGGAGTCCAG CCTGGAGGTG GCAGTGCCAC CCGCAGTCGC CCCTCAGGCC
    GGTGCTTCGT TGCTCACTCC TTTCCCGACG CAAAGGGTGT GCTCCAACCC GCCCTGTGAG
    ACGCACGAGA CAGGCACTAC GCACACGGCC ACCACTGTCA CCTCCAACAT GAGTTCCAAC
    CAAGACACCC CACCGCCTGC CGGCGACCAG GGAGAGGTGG AGAGCACCCA GGGCGACAGT
    GTGAATATCG CCAGTTCCAG TCCCATCACG ACAACAGTGT CTTCCACGCT GACGCGGGCC
    GTGACCACCG TGACACAGTC CACGCCAGTC CCAGGCCCTT CGGTGCCGCC CCCAGAGGAG
    CTCCAGGCCT CTCCAGGGCC ACGCCAGCAG CTTCCACCAC GGCAGCTCCT GCAGCCTGCC
    TCCGCGCCCT TGGTGGGGGA CTCCGCCGAG GTCCTGTCAG CCTCCCAGAG CCCTGAGCTC
    CAGGCCGCCG TGGATCTGAG CAGTACAGGG GACCCGTCTT CGGGCCAGGA GCCTGCCAGC
    TCGGCTGTGG TGGCCACCGT GGTGGTCCAG CCACCGCCGC CTACCCAGTC CGAAGTAGAC
    CAGTTGTCTC TCCCCCAAGA GCTGATGGCC GAGGCCCAGG CGGGCACCAC CACCCTCATG
    GTGACAGGGC TCACCCCAGA GGAGCTGGCA GTGACTGCTG CTGCTGAAGC GGCCGCTCAG
    GCTGCAGCCA CAGAGGAGGC CCAGGCCCTG GCCATCCAGG CCGTGCTCCA GGCCGCACAG
    CAGGCTGTCA TGGGCACCGG GGAGCCCATG GATACTTCAG AGGCGGCCGC GGCGGTGACA
    CAGGCAGAGC TGAGCCACTT GTCAGCCGAG GGCCAGGAAG GCCAGGCGAC CACTATCCCC
    ATCGTGCTGA CGCAGCAGGA GCTGGCTGCC CTGGTCCAGC AGCAGCAGCT CCAGGAAGCA
    CAGGCCCAGC AGCAGCAGCA CCACCTGCCC ACGGAAGCGC TGGCCCCCGC TGACAGCCTC
    AATGACCCGA CCATCGAGAG CAACTGCCTC AATGAGCTGG CCGGGGCTGT GCCCAGCACC
    GTGGGCCTGC TGCCCCCCAC GGCCACTGAG AGCCTGGCCC CGTCCAACAC ATTTGTGGCC
    CCTCAGCCGG TCGTTGTGGC CAGCCCCGCG AAGCTGCAGG CCGCGGCCAC CCTGACGGAA
    GTGGCTAATG GCATTGAGTC CCTGGGTGTG AAGCCAGACC TGCCACCGCC ACCTAGCAAA
    GCCCCTGTAA AGAAGGAGAA CCAGTGGTTT GACGTGGGGG TCATTAAGGG TACCAATGTG
    ATGGTGACAC ACTATTTCCT GCCACCCGAA GACGCTGTCC CGACTGATGA TGACTCGGGC
    ACCGTGCCCG ACTACAACCA GCTAAAGAAG CAGGAGCTGC AGCCAGGCAC TGCCTATAAG
    TTCCGCGTCG CCGGGATCAA TGCCTGCGGC CGGGGGCCCT TCAGTGAGAT CTCAGCCTTT
    AAAACGTGTC TGCCTGGCTT CCCGGGGGCC CCGTGTGCCA TTAAAATCAG CAAAAGTCCA
    GACGGTGCTC ACCTCACCTG GGAGCCACCC TCTGTGACCT CCGGCAAGAT CATCGAGTAT
    TCAGTGTACC TGGCCATCCA GAGCGCGCAG GCCGGTGGTG AGACCAAGAG CTCGACCCCG
    GCGCAGCTGG CCTTCATGCG GGTGTACTGC GGGCCCAGCC CCTCCTGCCT CGTGCAGTCC
    TCCAGCCTCT CCAACGCCCA CATTGACTAC ACCACCAAGC CCGCCATCAT CTTCCGCATT
    GCTGCCCGCA ATGAGAAGGG CTACGGCCCA GCCACCCAAG TCAGGTGGTT GCAAGAAACC
    AGTAAAGACA GCTCTGGCGC CAAGCCGGCC AGCAAGCGGC CCATGTCGTC TCCAGAAATG
    AAATCCGCGC CAAAGAAATC GAAGGCAGAC GGTCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.