TRIP12 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006047096.1	XP_006047158.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_025278387.1	XP_025134172.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025278394.1	XP_025134179.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_025278398.1	XP_025134183.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_025278403.1	XP_025134188.1	E3 ubiquitin-protein ligase TRIP12 isoform X17
XM_006047092.1	XP_006047154.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_025278386.1	XP_025134171.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025278397.1	XP_025134182.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_006047093.2	XP_006047155.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_025278399.1	XP_025134184.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
XM_025278400.1	XP_025134185.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_025278404.1	XP_025134189.1	E3 ubiquitin-protein ligase TRIP12 isoform X18
XM_006047097.1	XP_006047159.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_025278388.1	XP_025134173.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_025278390.1	XP_025134175.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_025278391.1	XP_025134176.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_025278392.1	XP_025134177.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_006047095.2	XP_006047157.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
XM_025278405.1	XP_025134190.1	E3 ubiquitin-protein ligase TRIP12 isoform X19
XM_006047090.1	XP_006047152.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_006047091.1	XP_006047153.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_006047093.1	XP_006047155.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_006047095.1	XP_006047157.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_006047088.2	XP_006047150.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025278389.1	XP_025134174.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_006047088.1	XP_006047150.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_006047089.1	XP_006047151.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_006047094.1	XP_006047156.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_025278395.1	XP_025134180.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_025278396.1	XP_025134181.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_025278401.1	XP_025134186.1	E3 ubiquitin-protein ligase TRIP12 isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu05780	XM_006047096.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu05781	XM_006047097.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu56078	XM_025278403.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56079	XM_025278404.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56080	XM_025278405.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu05772	XM_006047088.2 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05772	XM_025278386.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05772	XM_025278387.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05773	XM_025278391.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05773	XM_006047089.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05774	XM_025278395.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05774	XM_006047090.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05775	XM_025278396.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05775	XM_006047091.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05776	XM_025278397.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05776	XM_006047092.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05777	XM_006047093.2 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05778	XM_006047094.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05778	XM_025278400.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05779	XM_006047095.2 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu56070	XM_025278388.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56071	XM_025278389.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56072	XM_025278390.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56073	XM_025278392.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56074	XM_025278394.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56075	XM_025278398.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56076	XM_025278399.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu56077	XM_025278401.1 Latest version!	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBu05777	XM_006047093.1	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05779	XM_006047095.1	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu05772	XM_006047088.1	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

TRIP12 ( XM_006047096.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047158.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu05780
Clone ID Related Accession (Same CDS sequence)	XM_006047096.1
Accession Version	XM_006047096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGGGGCTG TGGGCATGAC TACCTCTGGG GAGAGCGAAT CTGATGACTC CGAGATGGGA 
CGGTTACAAG CTCTGTTAGA GGCCAGGGGT CTTCCCCCTC ACCTGTTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTGC AAGGACTACA AGCAAGCGAT GAAAGCCAGC AGCTCCAGGC CGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGGAATGAG GAGACGCTGG GAGGGTTTCC TGTCAAGAGT 
GTGGTTCCAG CTTTGATAAC ATTGCTGCAG ATGGAACATA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCATCAGC TGTGGTAGTA 
GATGCAATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCCGAA 
CAGGCCTTGA CAGCCTTGGA GATGTTATCA CGTAGACACA GTAAAGCCAT TCTCCAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTATCTA GAGTTCTTCA GCATAAATGC CCAAAGAAAC 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCGGATTCCC TCCCATTGCT TACCCAAAGG CTAACCCACC AGGACAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGTCTAGTG GACAACTTCC AACATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTAACA AATGTTCAAC AACTGTTGGT AGTAACTCCA 
CCAATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AATTGTCCAA CTTTAGCAGT TCAACTTATG AAGCAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAGGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCCTTAG CCAATACTAA CACTAGTGGA TATTCAGAGT TAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTCGCGGAT GCCGAACTTC TAAAGGATGT CCTGAAAAAC 
CATGCTGTTT CAAGTCATAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTT 
GGAGCCCTTC AAATGGCAGA GATCTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCTGA GTCATTGTTG 
ACGAGTCCAC CAAAGGCATG TACAAATGGG TCCGGATCCT TGGGGTCCAC ACCATCGGTC 
AACAGTGGAA CAGCCACGGC TGCCACCAAT GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG 
CAGCACAGCA GAGACGATTC TCTAGACCTG AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGATCA AGAAGGCCAA AGTATTCACC TCCGAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAGTCTTCT 
TTCTTGGCAA GCTTGAATCC AAAAACGTGG GGAAGGTTAA GCGCACAGTC TAACAGCAAC 
AACATTGAAC CAGCACGAAC TGCGGGAGTT AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AAGAGCAGGC ACATAAATTT 
GTAGAGCGTT ACTTCAGTTC TGAGAACATG GATGGAAGCA ACCCCGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCGAC TGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCTGATGTTT CATCGTTTGA AATCCAACAC 
AGTGGATTTG TGAAGCAGCT GTTGCTCTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GAGATTCCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGAACGAGT AGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAAATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTGCA TGACTTTCCC 
AGTGGAAATG GGACAGGAGG CAGCTTCTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAGTTAAA ATGCCAGTTA CAAAGACATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCCCTGGCTT TGGTGCAGGC TATTGAGAGA 
TATCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ACGAAGACAG TGACGACGAT 
GGCTCTGATG AGGAAATAGA CGAATCTCTG GCTGCTCAGT TCTTAAATTC CGGAAATGTG 
AGACACAGGC TACAGTTTTA CATCGGAGAG CATTTGCTAC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTCAG TATACAGGCT GAAGATGAAC GGGAGTCCAC GGATGACGAG 
AGCAATCCTC TAGGAAGAGC TGGTATTTGG ACGAAAACTC ATACGATATG GTACAAACCT 
GTGCGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG 
ACAGCTCCAA CAAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGCT GTGGAATGAT 
GGAGTGTGCC CGTCCGTATC AAATCCTTTA GAAGTTTACC TCATACCCAC AGCACCCGAA 
AATATAACTT TTGAAGACCC GTCATTAGAT GTAATTCTTC TTTTAAGAGT TTTACACGCC 
GTCAGTAGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAGAT TATCCCAACT 
AGTGAATTTA TTAACAGTAA GTTGACAGCA AAAGCAAATA GGCAGCTTCA AGATCCTTTG 
GTAATCATGA CAGGAAACAT CCCTACATGG CTCACTGAAC TAGGAAAAAC CTGCCCATTT 
TTCTTCCCTT TTGATACCCG GCAAATGCTT TTCTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAGATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA CAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTGCTGAAA 
CAAGCGGAGT CTGTGATGCA GGATCTTGGC AGCTCAAGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGCACAGG TCTTGGGCCT ACACTGGAGT TTTATGCACT TGTATCTCAG 
GAACTGCAGA GAGCTGACTT GGGTCTCTGG AGAGGGGAGG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AAGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCATATC GCAAAGGTTA AAATGAAGTT TCGCTTCTTA 
GGAAAACTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTGGGCTTA 
CCTTTTTATA AATGGATGCT CCGGCAAGAA ACTTCGCTGA CGTCACATGA TTTGTTTGAC 
ATTGACCCAG TTGTAGCCAG GTCAGTGTAT CACCTCGAAG ACATCGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGCCTCC AATATGCATT AGAAACCCTG 
ACTATGAACG GCTGCTCCGT GGAGGATCTG GGATTGGATT TCACTCTGCC AGGGTTTCCT 
AACATTGAAC TAAAGAAAGG AGGAAAGGAT ATACCGGTCA CCATCCACAA TTTGGAGGAG 
TATCTAAGAC TGGTTATATT TTGGGCACTA AACGAAGGTG TTTCCAGGCA ATTTGATTCA 
TTTAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TCCAGTACTT CTACCCAGAA 
GAACTGGATC AGCTTCTGTG TGGTAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGCA GGCCAGATCA CGGTTACACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA CAATGAACAG CAGAGGTTAT TTCTCCAGTT TGTGACAGGT 
AGCCCACGGT TGCCTGTTGG AGGCTTCCGG AGCTTGAATC CACCTTTGAC CATTGTCCGG 
AAGACCTTCG AATCAACAGA GAACCCAGAT GACTTCCTAC CCTCTGTCAT GACCTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC CTTGAGATAA TGCGGGAAAA GCTGTTGATG 
GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047158.1
CDS	50..6028
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047096.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047096.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGGGGCTG TGGGCATGAC TACCTCTGGG GAGAGCGAAT CTGATGACTC CGAGATGGGA 
CGGTTACAAG CTCTGTTAGA GGCCAGGGGT CTTCCCCCTC ACCTGTTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTTCTGC AAGGACTACA AGCAAGCGAT GAAAGCCAGC AGCTCCAGGC CGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGGAATGAG GAGACGCTGG GAGGGTTTCC TGTCAAGAGT 
GTGGTTCCAG CTTTGATAAC ATTGCTGCAG ATGGAACATA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCATCAGC TGTGGTAGTA 
GATGCAATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCCGAA 
CAGGCCTTGA CAGCCTTGGA GATGTTATCA CGTAGACACA GTAAAGCCAT TCTCCAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTATCTA GAGTTCTTCA GCATAAATGC CCAAAGAAAC 
GCACTAGCAA TTGCAGCCAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCGGATTCCC TCCCATTGCT TACCCAAAGG CTAACCCACC AGGACAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGTCTAGTG GACAACTTCC AACATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTAACA AATGTTCAAC AACTGTTGGT AGTAACTCCA 
CCAATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AATTGTCCAA CTTTAGCAGT TCAACTTATG AAGCAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAGCAGA TTGATCTTGT TCCACGAAGT 
CCTCAAGAAC TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAG 
GAAGGCATTT TTGCAGTTGA TACCATGCTG AAGAAGGGCA ATGCACAGAA CACGGATGGG 
GCCATATGGC AGTGGCGGGA TGACCGGGGC CTCTGGCACC CCTATAACAG GATAGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCCTTAG CCAATACTAA CACTAGTGGA TATTCAGAGT TAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTCGCGGAT GCCGAACTTC TAAAGGATGT CCTGAAAAAC 
CATGCTGTTT CAAGTCATAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTT 
GGAGCCCTTC AAATGGCAGA GATCTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCTGA GTCATTGTTG 
ACGAGTCCAC CAAAGGCATG TACAAATGGG TCCGGATCCT TGGGGTCCAC ACCATCGGTC 
AACAGTGGAA CAGCCACGGC TGCCACCAAT GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG 
CAGCACAGCA GAGACGATTC TCTAGACCTG AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGATCA AGAAGGCCAA AGTATTCACC TCCGAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAGTCTTCT 
TTCTTGGCAA GCTTGAATCC AAAAACGTGG GGAAGGTTAA GCGCACAGTC TAACAGCAAC 
AACATTGAAC CAGCACGAAC TGCGGGAGTT AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AAGAGCAGGC ACATAAATTT 
GTAGAGCGTT ACTTCAGTTC TGAGAACATG GATGGAAGCA ACCCCGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCGAC TGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCTGATGTTT CATCGTTTGA AATCCAACAC 
AGTGGATTTG TGAAGCAGCT GTTGCTCTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GAGATTCCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGAACGAGT AGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAAATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTGCA TGACTTTCCC 
AGTGGAAATG GGACAGGAGG CAGCTTCTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAGTTAAA ATGCCAGTTA CAAAGACATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCCCTGGCTT TGGTGCAGGC TATTGAGAGA 
TATCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ACGAAGACAG TGACGACGAT 
GGCTCTGATG AGGAAATAGA CGAATCTCTG GCTGCTCAGT TCTTAAATTC CGGAAATGTG 
AGACACAGGC TACAGTTTTA CATCGGAGAG CATTTGCTAC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTCAG TATACAGGCT GAAGATGAAC GGGAGTCCAC GGATGACGAG 
AGCAATCCTC TAGGAAGAGC TGGTATTTGG ACGAAAACTC ATACGATATG GTACAAACCT 
GTGCGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG 
ACAGCTCCAA CAAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGCT GTGGAATGAT 
GGAGTGTGCC CGTCCGTATC AAATCCTTTA GAAGTTTACC TCATACCCAC AGCACCCGAA 
AATATAACTT TTGAAGACCC GTCATTAGAT GTAATTCTTC TTTTAAGAGT TTTACACGCC 
GTCAGTAGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAGAT TATCCCAACT 
AGTGAATTTA TTAACAGTAA GTTGACAGCA AAAGCAAATA GGCAGCTTCA AGATCCTTTG 
GTAATCATGA CAGGAAACAT CCCTACATGG CTCACTGAAC TAGGAAAAAC CTGCCCATTT 
TTCTTCCCTT TTGATACCCG GCAAATGCTT TTCTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAGATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA CAGAAAAAAA CGTACTGTGA ACAGAGAGGA GCTGCTGAAA 
CAAGCGGAGT CTGTGATGCA GGATCTTGGC AGCTCAAGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGCACAGG TCTTGGGCCT ACACTGGAGT TTTATGCACT TGTATCTCAG 
GAACTGCAGA GAGCTGACTT GGGTCTCTGG AGAGGGGAGG AAGTAACTCT TAGCAATCCA 
AAAGGAAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AAGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCTAAGCC AGCTCATATC GCAAAGGTTA AAATGAAGTT TCGCTTCTTA 
GGAAAACTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTGGGCTTA 
CCTTTTTATA AATGGATGCT CCGGCAAGAA ACTTCGCTGA CGTCACATGA TTTGTTTGAC 
ATTGACCCAG TTGTAGCCAG GTCAGTGTAT CACCTCGAAG ACATCGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGCCTCC AATATGCATT AGAAACCCTG 
ACTATGAACG GCTGCTCCGT GGAGGATCTG GGATTGGATT TCACTCTGCC AGGGTTTCCT 
AACATTGAAC TAAAGAAAGG AGGAAAGGAT ATACCGGTCA CCATCCACAA TTTGGAGGAG 
TATCTAAGAC TGGTTATATT TTGGGCACTA AACGAAGGTG TTTCCAGGCA ATTTGATTCA 
TTTAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TCCAGTACTT CTACCCAGAA 
GAACTGGATC AGCTTCTGTG TGGTAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGCA GGCCAGATCA CGGTTACACT CATGACAGTC GGGCTGTGAA GTTCTTGTTT 
GAGATTCTCA GTAGTTTTGA CAATGAACAG CAGAGGTTAT TTCTCCAGTT TGTGACAGGT 
AGCCCACGGT TGCCTGTTGG AGGCTTCCGG AGCTTGAATC CACCTTTGAC CATTGTCCGG 
AAGACCTTCG AATCAACAGA GAACCCAGAT GACTTCCTAC CCTCTGTCAT GACCTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC CTTGAGATAA TGCGGGAAAA GCTGTTGATG 
GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047097.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047159.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OBu05781
Clone ID Related Accession (Same CDS sequence)	XM_006047097.1
Accession Version	XM_006047097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAGATGG CCGACCCTGA AGGCAACCAG 
GAGACGGTGA ACTCTTCAGC TGCACGGACA GATGAGACTC CCCAAGGAGC AGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT GGGCATGACT ACCTCTGGGG AGAGCGAATC TGATGACTCC 
GAGATGGGAC GGTTACAAGC TCTGTTAGAG GCCAGGGGTC TTCCCCCTCA CCTGTTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTTCTGCA AGGACTACAA GCAAGCGATG AAAGCCAGCA GCTCCAGGCC 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGGAATGAGG AGACGCTGGG AGGGTTTCCT 
GTCAAGAGTG TGGTTCCAGC TTTGATAACA TTGCTGCAGA TGGAACATAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCATCAGCT 
GTGGTAGTAG ATGCAATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCCGAAC AGGCCTTGAC AGCCTTGGAG ATGTTATCAC GTAGACACAG TAAAGCCATT 
CTCCAGGCGG GTGGTTTGGC AGACTGCTTG CTGTATCTAG AGTTCTTCAG CATAAATGCC 
CAAAGAAACG CACTAGCAAT TGCAGCCAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CGGATTCCCT CCCATTGCTT ACCCAAAGGC TAACCCACCA GGACAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGTCTAGTGG ACAACTTCCA ACATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTAACAA ATGTTCAACA ACTGTTGGTA 
GTAACTCCAC CAATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ATTGTCCAAC TTTAGCAGTT CAACTTATGA AGCAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAGCAGAT TGATCTTGTT 
CCACGAAGTC CTCAAGAACT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAGG AAGGCATTTT TGCAGTTGAT ACCATGCTGA AGAAGGGCAA TGCACAGAAC 
ACGGATGGGG CCATATGGCA GTGGCGGGAT GACCGGGGCC TCTGGCACCC CTATAACAGG 
ATAGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047159.1
CDS	50..5218
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGSRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047097.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047097.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAGATGG CCGACCCTGA AGGCAACCAG 
GAGACGGTGA ACTCTTCAGC TGCACGGACA GATGAGACTC CCCAAGGAGC AGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT GGGCATGACT ACCTCTGGGG AGAGCGAATC TGATGACTCC 
GAGATGGGAC GGTTACAAGC TCTGTTAGAG GCCAGGGGTC TTCCCCCTCA CCTGTTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTTCTGCA AGGACTACAA GCAAGCGATG AAAGCCAGCA GCTCCAGGCC 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGGAATGAGG AGACGCTGGG AGGGTTTCCT 
GTCAAGAGTG TGGTTCCAGC TTTGATAACA TTGCTGCAGA TGGAACATAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCATCAGCT 
GTGGTAGTAG ATGCAATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCCGAAC AGGCCTTGAC AGCCTTGGAG ATGTTATCAC GTAGACACAG TAAAGCCATT 
CTCCAGGCGG GTGGTTTGGC AGACTGCTTG CTGTATCTAG AGTTCTTCAG CATAAATGCC 
CAAAGAAACG CACTAGCAAT TGCAGCCAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CGGATTCCCT CCCATTGCTT ACCCAAAGGC TAACCCACCA GGACAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGTCTAGTGG ACAACTTCCA ACATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTAACAA ATGTTCAACA ACTGTTGGTA 
GTAACTCCAC CAATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ATTGTCCAAC TTTAGCAGTT CAACTTATGA AGCAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAGCAGAT TGATCTTGTT 
CCACGAAGTC CTCAAGAACT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAGG AAGGCATTTT TGCAGTTGAT ACCATGCTGA AGAAGGGCAA TGCACAGAAC 
ACGGATGGGG CCATATGGCA GTGGCGGGAT GACCGGGGCC TCTGGCACCC CTATAACAGG 
ATAGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278403.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134188.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

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CloneID	OBu56078
Clone ID Related Accession (Same CDS sequence)	XM_025278403.1
Accession Version	XM_025278403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATATCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGCTCTG ATGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGA GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCGTCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAA GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGCC TCCAATATGC ATTAGAAACC 
CTGACTATGA ACGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAACGAAG GTGTTTCCAG GCAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTTGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134188.1
CDS	1..5982
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278403.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278403.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATATCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGCTCTG ATGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGA GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCGTCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAA GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGCC TCCAATATGC ATTAGAAACC 
CTGACTATGA ACGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAACGAAG GTGTTTCCAG GCAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTTGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278404.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134189.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

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CloneID	OBu56079
Clone ID Related Accession (Same CDS sequence)	XM_025278404.1
Accession Version	XM_025278404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
GCTTCCGGAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134189.1
CDS	180..6014
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEEASGA

Target ORF information:

RefSeq Version	XM_025278404.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278404.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
GCTTCCGGAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278405.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134190.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

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CloneID	OBu56080
Clone ID Related Accession (Same CDS sequence)	XM_025278405.1
Accession Version	XM_025278405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA TCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG CTCTGATGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGAGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCG TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCAAGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAGGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGCCTCCAA 
TATGCATTAG AAACCCTGAC TATGAACGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA CGAAGGTGTT 
TCCAGGCAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGGTTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT TGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134190.1
CDS	50..6046
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278405.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278405.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GCTTCTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC CCTGGCTTTG 
GTGCAGGCTA TTGAGAGATA TCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAC 
GAAGACAGTG ACGACGATGG CTCTGATGAG GAAATAGACG AATCTCTGGC TGCTCAGTTC 
TTAAATTCCG GAAATGTGAG ACACAGGCTA CAGTTTTACA TCGGAGAGCA TTTGCTACCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTCAGTA TACAGGCTGA AGATGAACGG 
GAGTCCACGG ATGACGAGAG CAATCCTCTA GGAAGAGCTG GTATTTGGAC GAAAACTCAT 
ACGATATGGT ACAAACCTGT GCGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAGAC AGCTCCAACA AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGCTGT GGAATGATGG AGTGTGCCCG TCCGTATCAA ATCCTTTAGA AGTTTACCTC 
ATACCCACAG CACCCGAAAA TATAACTTTT GAAGACCCGT CATTAGATGT AATTCTTCTT 
TTAAGAGTTT TACACGCCGT CAGTAGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TCCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTGGT AATCATGACA GGAAACATCC CTACATGGCT CACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT CTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA GATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGACA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTGATGCAGG ATCTTGGCAG CTCAAGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGCACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTGCAGAGA GCTGACTTGG GTCTCTGGAG AGGGGAGGAA 
GTAACTCTTA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAA 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCTAAGCCAG CTCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAACTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TGGGCTTACC TTTTTATAAA TGGATGCTCC GGCAAGAAAC TTCGCTGACG 
TCACATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGGT CAGTGTATCA CCTCGAAGAC 
ATCGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGCCTCCAA 
TATGCATTAG AAACCCTGAC TATGAACGGC TGCTCCGTGG AGGATCTGGG ATTGGATTTC 
ACTCTGCCAG GGTTTCCTAA CATTGAACTA AAGAAAGGAG GAAAGGATAT ACCGGTCACC 
ATCCACAATT TGGAGGAGTA TCTAAGACTG GTTATATTTT GGGCACTAAA CGAAGGTGTT 
TCCAGGCAAT TTGATTCATT TAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTC 
CAGTACTTCT ACCCAGAAGA ACTGGATCAG CTTCTGTGTG GTAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGCAGG CCAGATCACG GTTACACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGACA ATGAACAGCA GAGGTTATTT 
CTCCAGTTTG TGACAGGTAG CCCACGGTTG CCTGTTGGAG GCTTCCGGAG CTTGAATCCA 
CCTTTGACCA TTGTCCGGAA GACCTTCGAA TCAACAGAGA ACCCAGATGA CTTCCTACCC 
TCTGTCATGA CCTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCCT TGAGATAATG 
CGGGAAAAGC TGTTGATGGC TGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278387.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134172.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OBu05772
Clone ID Related Accession (Same CDS sequence)	XM_025278387.1 , XM_025278386.1 , XM_006047088.2 , XM_006047088.1
Accession Version	XM_025278387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134172.1
CDS	72..6287
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278387.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278387.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278386.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134171.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OBu05772
Clone ID Related Accession (Same CDS sequence)	XM_025278387.1 , XM_025278386.1 , XM_006047088.2 , XM_006047088.1
Accession Version	XM_025278386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134171.1
CDS	180..6395
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278386.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278386.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047088.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047150.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OBu05772
Clone ID Related Accession (Same CDS sequence)	XM_025278387.1 , XM_025278386.1 , XM_006047088.2 , XM_006047088.1
Accession Version	XM_006047088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047150.1
CDS	70..6285
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047088.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047088.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047088.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047150.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OBu05772
Clone ID Related Accession (Same CDS sequence)	XM_025278387.1 , XM_025278386.1 , XM_006047088.2 , XM_006047088.1
Accession Version	XM_006047088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047150.1
CDS	72..6287
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047088.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047088.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG CTTCTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AGTTAAAATG CCAGTTACAA AGACATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCC CTGGCTTTGG TGCAGGCTAT TGAGAGATAT 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGACG AAGACAGTGA CGACGATGGC 
TCTGATGAGG AAATAGACGA ATCTCTGGCT GCTCAGTTCT TAAATTCCGG AAATGTGAGA 
CACAGGCTAC AGTTTTACAT CGGAGAGCAT TTGCTACCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTCAGTAT ACAGGCTGAA GATGAACGGG AGTCCACGGA TGACGAGAGC 
AATCCTCTAG GAAGAGCTGG TATTTGGACG AAAACTCATA CGATATGGTA CAAACCTGTG 
CGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAGACA 
GCTCCAACAA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGCTGTG GAATGATGGA 
GTGTGCCCGT CCGTATCAAA TCCTTTAGAA GTTTACCTCA TACCCACAGC ACCCGAAAAT 
ATAACTTTTG AAGACCCGTC ATTAGATGTA ATTCTTCTTT TAAGAGTTTT ACACGCCGTC 
AGTAGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAGATTAT CCCAACTAGT 
GAATTTATTA ACAGTAAGTT GACAGCAAAA GCAAATAGGC AGCTTCAAGA TCCTTTGGTA 
ATCATGACAG GAAACATCCC TACATGGCTC ACTGAACTAG GAAAAACCTG CCCATTTTTC 
TTCCCTTTTG ATACCCGGCA AATGCTTTTC TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAG ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGACAG AAAAAAACGT ACTGTGAACA GAGAGGAGCT GCTGAAACAA 
GCGGAGTCTG TGATGCAGGA TCTTGGCAGC TCAAGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GCACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCACTTGT ATCTCAGGAA 
CTGCAGAGAG CTGACTTGGG TCTCTGGAGA GGGGAGGAAG TAACTCTTAG CAATCCAAAA 
GGAAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAAG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CTAAGCCAGC TCATATCGCA AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA 
AAACTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT GGGCTTACCT 
TTTTATAAAT GGATGCTCCG GCAAGAAACT TCGCTGACGT CACATGATTT GTTTGACATT 
GACCCAGTTG TAGCCAGGTC AGTGTATCAC CTCGAAGACA TCGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGCCTCCAAT ATGCATTAGA AACCCTGACT 
ATGAACGGCT GCTCCGTGGA GGATCTGGGA TTGGATTTCA CTCTGCCAGG GTTTCCTAAC 
ATTGAACTAA AGAAAGGAGG AAAGGATATA CCGGTCACCA TCCACAATTT GGAGGAGTAT 
CTAAGACTGG TTATATTTTG GGCACTAAAC GAAGGTGTTT CCAGGCAATT TGATTCATTT 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTCC AGTACTTCTA CCCAGAAGAA 
CTGGATCAGC TTCTGTGTGG TAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGCAGGC CAGATCACGG TTACACTCAT GACAGTCGGG CTGTGAAGTT CTTGTTTGAG 
ATTCTCAGTA GTTTTGACAA TGAACAGCAG AGGTTATTTC TCCAGTTTGT GACAGGTAGC 
CCACGGTTGC CTGTTGGAGG CTTCCGGAGC TTGAATCCAC CTTTGACCAT TGTCCGGAAG 
ACCTTCGAAT CAACAGAGAA CCCAGATGAC TTCCTACCCT CTGTCATGAC CTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCCTT GAGATAATGC GGGAAAAGCT GTTGATGGCT 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278391.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134176.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OBu05773
Clone ID Related Accession (Same CDS sequence)	XM_025278391.1 , XM_006047089.1
Accession Version	XM_025278391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATATCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGCTCTGA TGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGAG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCGTCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCAAG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA GGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGCCT CCAATATGCA TTAGAAACCC TGACTATGAA CGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAACGAAGG TGTTTCCAGG CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG GTTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTTGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134176.1
CDS	180..6380
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278391.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278391.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATATCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGCTCTGA TGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGAG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCGTCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCAAG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA GGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGCCT CCAATATGCA TTAGAAACCC TGACTATGAA CGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAACGAAGG TGTTTCCAGG CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG GTTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTTGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047089.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047151.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OBu05773
Clone ID Related Accession (Same CDS sequence)	XM_025278391.1 , XM_006047089.1
Accession Version	XM_006047089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATATCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGCTCTGA TGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGAG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCGTCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCAAG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA GGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGCCT CCAATATGCA TTAGAAACCC TGACTATGAA CGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAACGAAGG TGTTTCCAGG CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG GTTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTTGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047151.1
CDS	50..6250
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047089.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047089.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTAGC AGCCCATCAG 
GTCGGTGAGG ATGAGATAAG CTTGTCCACT CTGGGACGAG TTTATACTAT TGATTTTAAT 
TCTATGCAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCCTTAGCCA ATACTAACAC TAGTGGATAT TCAGAGTTAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT CGCGGATGCC GAACTTCTAA AGGATGTCCT GAAAAACCAT 
GCTGTTTCAA GTCATATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA 
GCCCTTCAAA TGGCAGAGAT CTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCTGAGTC ATTGTTGACG 
AGTCCACCAA AGGCATGTAC AAATGGGTCC GGATCCTTGG GGTCCACACC ATCGGTCAAC 
AGTGGAACAG CCACGGCTGC CACCAATGCC TCGGCTGACC TGGGGTCCCC CAGCTTGCAG 
CACAGCAGAG ACGATTCTCT AGACCTGAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGATCAAGA AGGCCAAAGT ATTCACCTCC GAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA GTCTTCTTTC 
TTGGCAAGCT TGAATCCAAA AACGTGGGGA AGGTTAAGCG CACAGTCTAA CAGCAACAAC 
ATTGAACCAG CACGAACTGC GGGAGTTAGT GGTCTTGCCA GGGCCGCCTC AAAGGACACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAAG AGCAGGCACA TAAATTTGTA 
GAGCGTTACT TCAGTTCTGA GAACATGGAT GGAAGCAACC CCGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCGACTGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCT GATGTTTCAT CGTTTGAAAT CCAACACAGT 
GGATTTGTGA AGCAGCTGTT GCTCTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGAG ATTCCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG AACGAGTAGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAA 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTGCATGA CTTTCCCAGT 
GGAAATGGGA CAGGAGGCAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCCCTGGC TTTGGTGCAG GCTATTGAGA GATATCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGACGAAGAC AGTGACGACG ATGGCTCTGA TGAGGAAATA 
GACGAATCTC TGGCTGCTCA GTTCTTAAAT TCCGGAAATG TGAGACACAG GCTACAGTTT 
TACATCGGAG AGCATTTGCT ACCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTC 
AGTATACAGG CTGAAGATGA ACGGGAGTCC ACGGATGACG AGAGCAATCC TCTAGGAAGA 
GCTGGTATTT GGACGAAAAC TCATACGATA TGGTACAAAC CTGTGCGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AGACAGCTCC AACAAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG CTGTGGAATG ATGGAGTGTG CCCGTCCGTA 
TCAAATCCTT TAGAAGTTTA CCTCATACCC ACAGCACCCG AAAATATAAC TTTTGAAGAC 
CCGTCATTAG ATGTAATTCT TCTTTTAAGA GTTTTACACG CCGTCAGTAG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATCCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TGGTAATCAT GACAGGAAAC 
ATCCCTACAT GGCTCACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTCTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAGATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GACAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTGATG 
CAGGATCTTG GCAGCTCAAG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGCACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTGCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGGGA GGAAGTAACT CTTAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAAGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCTAAG 
CCAGCTCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAACT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTGGGCT TACCTTTTTA TAAATGGATG 
CTCCGGCAAG AAACTTCGCT GACGTCACAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGGTCAGTGT ATCACCTCGA AGACATCGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGCCT CCAATATGCA TTAGAAACCC TGACTATGAA CGGCTGCTCC 
GTGGAGGATC TGGGATTGGA TTTCACTCTG CCAGGGTTTC CTAACATTGA ACTAAAGAAA 
GGAGGAAAGG ATATACCGGT CACCATCCAC AATTTGGAGG AGTATCTAAG ACTGGTTATA 
TTTTGGGCAC TAAACGAAGG TGTTTCCAGG CAATTTGATT CATTTAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTCCAGTAC TTCTACCCAG AAGAACTGGA TCAGCTTCTG 
TGTGGTAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG CAGGCCAGAT 
CACGGTTACA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GACAATGAAC AGCAGAGGTT ATTTCTCCAG TTTGTGACAG GTAGCCCACG GTTGCCTGTT 
GGAGGCTTCC GGAGCTTGAA TCCACCTTTG ACCATTGTCC GGAAGACCTT CGAATCAACA 
GAGAACCCAG ATGACTTCCT ACCCTCTGTC ATGACCTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCCTTGAGAT AATGCGGGAA AAGCTGTTGA TGGCTGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047090.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047152.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OBu05774
Clone ID Related Accession (Same CDS sequence)	XM_006047090.1 , XM_025278395.1
Accession Version	XM_006047090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTGT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACGA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACACCA TCGGTCAACA GTGGAACAGC CACGGCTGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC CGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCGACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAGCTGTTG 
CTCTATTTGA CATCTAAAAG TGAAAAGGAT GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATATC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGCT CTGATGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGAGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCGTC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CAAGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAGGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACATGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GCCTCCAATA TGCATTAGAA ACCCTGACTA TGAACGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAACG AAGGTGTTTC CAGGCAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGGTTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTTG AGATAATGCG GGAAAAGCTG TTGATGGCTG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047152.1
CDS	50..6184
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047090.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047090.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTGT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACGA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACACCA TCGGTCAACA GTGGAACAGC CACGGCTGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC CGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCGACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAGCTGTTG 
CTCTATTTGA CATCTAAAAG TGAAAAGGAT GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATATC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGCT CTGATGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGAGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCGTC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CAAGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAGGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACATGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GCCTCCAATA TGCATTAGAA ACCCTGACTA TGAACGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAACG AAGGTGTTTC CAGGCAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGGTTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTTG AGATAATGCG GGAAAAGCTG TTGATGGCTG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278395.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134180.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OBu05774
Clone ID Related Accession (Same CDS sequence)	XM_006047090.1 , XM_025278395.1
Accession Version	XM_025278395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTGT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACGA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACACCA TCGGTCAACA GTGGAACAGC CACGGCTGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC CGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCGACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAGCTGTTG 
CTCTATTTGA CATCTAAAAG TGAAAAGGAT GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATATC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGCT CTGATGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGAGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCGTC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CAAGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAGGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACATGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GCCTCCAATA TGCATTAGAA ACCCTGACTA TGAACGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAACG AAGGTGTTTC CAGGCAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGGTTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTTG AGATAATGCG GGAAAAGCTG TTGATGGCTG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134180.1
CDS	180..6314
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278395.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278395.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGTACA AATCAATGAG 
GACACGGGAA CAGCACGTGC CATTCAGAGA AAACCTAACC CCTTAGCCAA TACTAACACT 
AGTGGATATT CAGAGTTAAA GAAGGATGAT GCTCGAGCAC AGCTTATGAA AGAGGATCCG 
GAACTGGCTA AGTCTTTTAT TAAGACATTA TTTGGTGTTC TTTATGAAGT GTATAGTTCC 
TCAGCAGGAC CTGCGGTCAG ACATAAGTGC CTTAGAGCAA TTCTTAGGAT AATTTATTTC 
GCGGATGCCG AACTTCTAAA GGATGTCCTG AAAAACCATG CTGTTTCAAG TCATATTGCT 
TCCATGCTGT CAAGCCAAGA CCTGAAGATA GTAGTTGGAG CCCTTCAAAT GGCAGAGATC 
TTAATGCAGA AGTTACCTGA TATTTTTAGT GTTTACTTCA GAAGAGAAGG TGTAATGCAT 
CAAGTAAAAC ACTTAGCAGA ATCTGAGTCA TTGTTGACGA GTCCACCAAA GGCATGTACA 
AATGGGTCCG GATCCTTGGG GTCCACACCA TCGGTCAACA GTGGAACAGC CACGGCTGCC 
ACCAATGCCT CGGCTGACCT GGGGTCCCCC AGCTTGCAGC ACAGCAGAGA CGATTCTCTA 
GACCTGAGCC CTCAAGGTCG ATTAAGTGAT GTTCTAAAGA GAAAACGACT GCCAAAACGA 
GGATCAAGAA GGCCAAAGTA TTCACCTCCG AGAGATGATG ACAAAGTAGA CAATCAAGCT 
AAAAGCCCCA CCACTACTCA GTCACCTAAG TCTTCTTTCT TGGCAAGCTT GAATCCAAAA 
ACGTGGGGAA GGTTAAGCGC ACAGTCTAAC AGCAACAACA TTGAACCAGC ACGAACTGCG 
GGAGTTAGTG GTCTTGCCAG GGCCGCCTCA AAGGACACCA TCTCCAATAA TAGAGAAAAA 
ATTAAAGGTT GGATTAAAGA GCAGGCACAT AAATTTGTAG AGCGTTACTT CAGTTCTGAG 
AACATGGATG GAAGCAACCC CGCATTGAAT GTCCTTCAGA GACTTTGTGC TGCGACTGAA 
CAACTCAACC TCCAGGTGGA TGGTGGAGCT GAGTGCCTTG TAGAAATCCG TAGCATAGTC 
TCAGAGTCTG ATGTTTCATC GTTTGAAATC CAACACAGTG GATTTGTGAA GCAGCTGTTG 
CTCTATTTGA CATCTAAAAG TGAAAAGGAT GCTGTGAGCA GAGAGATCAG ATTAAAGAGA 
TTCCTTCATG TATTTTTTTC TTCTCCACTT CCTGGAGAAG AGCCCATTGA ACGAGTAGAA 
CCAGTGGGTA ATGCACCTTT GTTGGCATTA GTTCACAAAA TGAACAACTG CCTCAGCCAG 
ATGGAACAAT TTCCAGTCAA AGTGCATGAC TTTCCCAGTG GAAATGGGAC AGGAGGCAGC 
TTCTCTCTCA ACAGAGGATC ACAGGCTTTA AAATTTTTCA ACACACATCA GTTAAAATGC 
CAGTTACAAA GACATCCAGA CTGTGCAAAT GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG 
ATTGACCCCC TGGCTTTGGT GCAGGCTATT GAGAGATATC TTGTAGTTAG AGGGTATGGA 
AGAGTAAGAG AAGATGACGA AGACAGTGAC GACGATGGCT CTGATGAGGA AATAGACGAA 
TCTCTGGCTG CTCAGTTCTT AAATTCCGGA AATGTGAGAC ACAGGCTACA GTTTTACATC 
GGAGAGCATT TGCTACCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTCAGTATA 
CAGGCTGAAG ATGAACGGGA GTCCACGGAT GACGAGAGCA ATCCTCTAGG AAGAGCTGGT 
ATTTGGACGA AAACTCATAC GATATGGTAC AAACCTGTGC GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAGACAG CTCCAACAAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGCTGTGG AATGATGGAG TGTGCCCGTC CGTATCAAAT 
CCTTTAGAAG TTTACCTCAT ACCCACAGCA CCCGAAAATA TAACTTTTGA AGACCCGTCA 
TTAGATGTAA TTCTTCTTTT AAGAGTTTTA CACGCCGTCA GTAGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAGATTATC CCAACTAGTG AATTTATTAA CAGTAAGTTG 
ACAGCAAAAG CAAATAGGCA GCTTCAAGAT CCTTTGGTAA TCATGACAGG AAACATCCCT 
ACATGGCTCA CTGAACTAGG AAAAACCTGC CCATTTTTCT TCCCTTTTGA TACCCGGCAA 
ATGCTTTTCT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAGA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGACAGA 
AAAAAACGTA CTGTGAACAG AGAGGAGCTG CTGAAACAAG CGGAGTCTGT GATGCAGGAT 
CTTGGCAGCT CAAGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG CACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCACTTGTA TCTCAGGAAC TGCAGAGAGC TGACTTGGGT 
CTCTGGAGAG GGGAGGAAGT AACTCTTAGC AATCCAAAAG GAAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAAGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC TAAGCCAGCT 
CATATCGCAA AGGTTAAAAT GAAGTTTCGC TTCTTAGGAA AACTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTG GGCTTACCTT TTTATAAATG GATGCTCCGG 
CAAGAAACTT CGCTGACGTC ACATGATTTG TTTGACATTG ACCCAGTTGT AGCCAGGTCA 
GTGTATCACC TCGAAGACAT CGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GCCTCCAATA TGCATTAGAA ACCCTGACTA TGAACGGCTG CTCCGTGGAG 
GATCTGGGAT TGGATTTCAC TCTGCCAGGG TTTCCTAACA TTGAACTAAA GAAAGGAGGA 
AAGGATATAC CGGTCACCAT CCACAATTTG GAGGAGTATC TAAGACTGGT TATATTTTGG 
GCACTAAACG AAGGTGTTTC CAGGCAATTT GATTCATTTA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTCCA GTACTTCTAC CCAGAAGAAC TGGATCAGCT TCTGTGTGGT 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGCAGGCC AGATCACGGT 
TACACTCATG ACAGTCGGGC TGTGAAGTTC TTGTTTGAGA TTCTCAGTAG TTTTGACAAT 
GAACAGCAGA GGTTATTTCT CCAGTTTGTG ACAGGTAGCC CACGGTTGCC TGTTGGAGGC 
TTCCGGAGCT TGAATCCACC TTTGACCATT GTCCGGAAGA CCTTCGAATC AACAGAGAAC 
CCAGATGACT TCCTACCCTC TGTCATGACC TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCCTTG AGATAATGCG GGAAAAGCTG TTGATGGCTG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047091.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047153.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OBu05775
Clone ID Related Accession (Same CDS sequence)	XM_006047091.1 , XM_025278396.1
Accession Version	XM_006047091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTGTCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACGAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACACCATCG GTCAACAGTG GAACAGCCAC GGCTGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCCGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC GACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA GCTGTTGCTC 
TATTTGACAT CTAAAAGTGA AAAGGATGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047153.1
CDS	50..6181
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047091.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047091.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTGTCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACGAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACACCATCG GTCAACAGTG GAACAGCCAC GGCTGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCCGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC GACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA GCTGTTGCTC 
TATTTGACAT CTAAAAGTGA AAAGGATGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278396.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134181.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OBu05775
Clone ID Related Accession (Same CDS sequence)	XM_006047091.1 , XM_025278396.1
Accession Version	XM_025278396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTGTCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACGAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACACCATCG GTCAACAGTG GAACAGCCAC GGCTGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCCGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC GACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA GCTGTTGCTC 
TATTTGACAT CTAAAAGTGA AAAGGATGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134181.1
CDS	180..6311
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278396.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278396.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGCAAAT CAATGAGGAC 
ACGGGAACAG CACGTGCCAT TCAGAGAAAA CCTAACCCCT TAGCCAATAC TAACACTAGT 
GGATATTCAG AGTTAAAGAA GGATGATGCT CGAGCACAGC TTATGAAAGA GGATCCGGAA 
CTGGCTAAGT CTTTTATTAA GACATTATTT GGTGTTCTTT ATGAAGTGTA TAGTTCCTCA 
GCAGGACCTG CGGTCAGACA TAAGTGCCTT AGAGCAATTC TTAGGATAAT TTATTTCGCG 
GATGCCGAAC TTCTAAAGGA TGTCCTGAAA AACCATGCTG TTTCAAGTCA TATTGCTTCC 
ATGCTGTCAA GCCAAGACCT GAAGATAGTA GTTGGAGCCC TTCAAATGGC AGAGATCTTA 
ATGCAGAAGT TACCTGATAT TTTTAGTGTT TACTTCAGAA GAGAAGGTGT AATGCATCAA 
GTAAAACACT TAGCAGAATC TGAGTCATTG TTGACGAGTC CACCAAAGGC ATGTACAAAT 
GGGTCCGGAT CCTTGGGGTC CACACCATCG GTCAACAGTG GAACAGCCAC GGCTGCCACC 
AATGCCTCGG CTGACCTGGG GTCCCCCAGC TTGCAGCACA GCAGAGACGA TTCTCTAGAC 
CTGAGCCCTC AAGGTCGATT AAGTGATGTT CTAAAGAGAA AACGACTGCC AAAACGAGGA 
TCAAGAAGGC CAAAGTATTC ACCTCCGAGA GATGATGACA AAGTAGACAA TCAAGCTAAA 
AGCCCCACCA CTACTCAGTC ACCTAAGTCT TCTTTCTTGG CAAGCTTGAA TCCAAAAACG 
TGGGGAAGGT TAAGCGCACA GTCTAACAGC AACAACATTG AACCAGCACG AACTGCGGGA 
GTTAGTGGTC TTGCCAGGGC CGCCTCAAAG GACACCATCT CCAATAATAG AGAAAAAATT 
AAAGGTTGGA TTAAAGAGCA GGCACATAAA TTTGTAGAGC GTTACTTCAG TTCTGAGAAC 
ATGGATGGAA GCAACCCCGC ATTGAATGTC CTTCAGAGAC TTTGTGCTGC GACTGAACAA 
CTCAACCTCC AGGTGGATGG TGGAGCTGAG TGCCTTGTAG AAATCCGTAG CATAGTCTCA 
GAGTCTGATG TTTCATCGTT TGAAATCCAA CACAGTGGAT TTGTGAAGCA GCTGTTGCTC 
TATTTGACAT CTAAAAGTGA AAAGGATGCT GTGAGCAGAG AGATCAGATT AAAGAGATTC 
CTTCATGTAT TTTTTTCTTC TCCACTTCCT GGAGAAGAGC CCATTGAACG AGTAGAACCA 
GTGGGTAATG CACCTTTGTT GGCATTAGTT CACAAAATGA ACAACTGCCT CAGCCAGATG 
GAACAATTTC CAGTCAAAGT GCATGACTTT CCCAGTGGAA ATGGGACAGG AGGCAGCTTC 
TCTCTCAACA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047092.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047154.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OBu05776
Clone ID Related Accession (Same CDS sequence)	XM_006047092.1 , XM_025278397.1
Accession Version	XM_006047092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AGGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047154.1
CDS	50..6178
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047092.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047092.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AGGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278397.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134182.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OBu05776
Clone ID Related Accession (Same CDS sequence)	XM_006047092.1 , XM_025278397.1
Accession Version	XM_025278397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AGGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134182.1
CDS	180..6308
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278397.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278397.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAGCTTTA CAGACATTGC AGAAACGCTT 
CACTTTCTCC TGTGTGGTGC CTCCAATGGA AGTTGTCAGG AGCAGATTGA TCTTGTTCCA 
CGAAGTCCTC AAGAACTGTA TGAACTGACA TCTCTGATTT GTGAACTTAT GCCATGTTTA 
CCAAAGGAAG GCATTTTTGC AGTTGATACC ATGCTGAAGA AGGGCAATGC ACAGAACACG 
GATGGGGCCA TATGGCAGTG GCGGGATGAC CGGGGCCTCT GGCACCCCTA TAACAGGATA 
GACAGCCGGA TCATTGAGGT AGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCCTTAG CCAATACTAA CACTAGTGGA 
TATTCAGAGT TAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTCGCGGAT 
GCCGAACTTC TAAAGGATGT CCTGAAAAAC CATGCTGTTT CAAGTCATAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTT GGAGCCCTTC AAATGGCAGA GATCTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCTGA GTCATTGTTG ACGAGTCCAC CAAAGGCATG TACAAATGGG 
TCCGGATCCT TGGGGTCCAC ACCATCGGTC AACAGTGGAA CAGCCACGGC TGCCACCAAT 
GCCTCGGCTG ACCTGGGGTC CCCCAGCTTG CAGCACAGCA GAGACGATTC TCTAGACCTG 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGATCA 
AGAAGGCCAA AGTATTCACC TCCGAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAGTCTTCT TTCTTGGCAA GCTTGAATCC AAAAACGTGG 
GGAAGGTTAA GCGCACAGTC TAACAGCAAC AACATTGAAC CAGCACGAAC TGCGGGAGTT 
AGTGGTCTTG CCAGGGCCGC CTCAAAGGAC ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AAGAGCAGGC ACATAAATTT GTAGAGCGTT ACTTCAGTTC TGAGAACATG 
GATGGAAGCA ACCCCGCATT GAATGTCCTT CAGAGACTTT GTGCTGCGAC TGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCTGATGTTT CATCGTTTGA AATCCAACAC AGTGGATTTG TGAAGCAGCT GTTGCTCTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GAGATTCCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGAACGAGT AGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAAATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTGCA TGACTTTCCC AGTGGAAATG GGACAGGAGG CAGCTTCTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047093.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047155.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

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CloneID	OBu05777
Clone ID Related Accession (Same CDS sequence)	XM_006047093.2 , XM_006047093.1
Accession Version	XM_006047093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACGAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACACCATC GGTCAACAGT GGAACAGCCA CGGCTGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCCG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CGACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AGCTGTTGCT CTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047155.1
CDS	1..6123
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047093.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047093.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACGAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACACCATC GGTCAACAGT GGAACAGCCA CGGCTGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCCG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CGACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AGCTGTTGCT CTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047093.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047155.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OBu05777
Clone ID Related Accession (Same CDS sequence)	XM_006047093.2 , XM_006047093.1
Accession Version	XM_006047093.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACGAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACACCATC GGTCAACAGT GGAACAGCCA CGGCTGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCCG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CGACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AGCTGTTGCT CTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047155.1
CDS	50..6172
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047093.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047093.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTCGC GGATGCCGAA 
CTTCTAAAGG ATGTCCTGAA AAACCATGCT GTTTCAAGTC ATATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCC CTTCAAATGG CAGAGATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CTGAGTCATT GTTGACGAGT CCACCAAAGG CATGTACAAA TGGGTCCGGA 
TCCTTGGGGT CCACACCATC GGTCAACAGT GGAACAGCCA CGGCTGCCAC CAATGCCTCG 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGACG ATTCTCTAGA CCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG ATCAAGAAGG 
CCAAAGTATT CACCTCCGAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAGTC TTCTTTCTTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCTAACAG CAACAACATT GAACCAGCAC GAACTGCGGG AGTTAGTGGT 
CTTGCCAGGG CCGCCTCAAA GGACACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAAGAGC AGGCACATAA ATTTGTAGAG CGTTACTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCCG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CGACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCGT TTGAAATCCA ACACAGTGGA TTTGTGAAGC AGCTGTTGCT CTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGAGATT CCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGAAC GAGTAGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAAATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TGCATGACTT TCCCAGTGGA AATGGGACAG GAGGCAGCTT CTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278400.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134185.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

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CloneID	OBu05778
Clone ID Related Accession (Same CDS sequence)	XM_025278400.1 , XM_006047094.1
Accession Version	XM_025278400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAGGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTGTCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACGAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CACCATCGGT CAACAGTGGA 
ACAGCCACGG CTGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCCGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCGA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAGC TGTTGCTCTA TTTGACATCT AAAAGTGAAA AGGATGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATATCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGCTCTGAT 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGAGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCGTCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCAAGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAG GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACATG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGCCTC CAATATGCAT TAGAAACCCT GACTATGAAC 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAACGAAGGT GTTTCCAGGC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGG 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTTGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134185.1
CDS	180..6269
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278400.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278400.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAGGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTGTCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACGAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CACCATCGGT CAACAGTGGA 
ACAGCCACGG CTGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCCGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCGA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAGC TGTTGCTCTA TTTGACATCT AAAAGTGAAA AGGATGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATATCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGCTCTGAT 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGAGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCGTCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCAAGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAG GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACATG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGCCTC CAATATGCAT TAGAAACCCT GACTATGAAC 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAACGAAGGT GTTTCCAGGC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGG 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTTGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047094.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047156.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OBu05778
Clone ID Related Accession (Same CDS sequence)	XM_025278400.1 , XM_006047094.1
Accession Version	XM_006047094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAGGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTGTCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACGAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CACCATCGGT CAACAGTGGA 
ACAGCCACGG CTGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCCGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCGA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAGC TGTTGCTCTA TTTGACATCT AAAAGTGAAA AGGATGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATATCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGCTCTGAT 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGAGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCGTCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCAAGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAG GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACATG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGCCTC CAATATGCAT TAGAAACCCT GACTATGAAC 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAACGAAGGT GTTTCCAGGC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGG 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTTGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047156.1
CDS	50..6139
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047094.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047094.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAGCTTT 
ACAGACATTG CAGAAACGCT TCACTTTCTC CTGTGTGGTG CCTCCAATGG AAGTTGTCAG 
GAGCAGATTG ATCTTGTTCC ACGAAGTCCT CAAGAACTGT ATGAACTGAC ATCTCTGATT 
TGTGAACTTA TGCCATGTTT ACCAAAGGAA GGCATTTTTG CAGTTGATAC CATGCTGAAG 
AAGGGCAATG CACAGAACAC GGATGGGGCC ATATGGCAGT GGCGGGATGA CCGGGGCCTC 
TGGCACCCCT ATAACAGGAT AGACAGCCGG ATCATTGAGG TAGCAGCCCA TCAGGTCGGT 
GAGGATGAGA TAAGCTTGTC CACTCTGGGA CGAGTTTATA CTATTGATTT TAATTCTATG 
CAGCAAATCA ATGAGGACAC GGGAACAGCA CGTGCCATTC AGAGAAAACC TAACCCCTTA 
GCCAATACTA ACACTAGTGG ATATTCAGAG TTAAAGAAGG ATGATGCTCG AGCACAGCTT 
ATGAAAGAGG ATCCGGAACT GGCTAAGTCT TTTATTAAGA CATTATTTGG TGTTCTTTAT 
GAAGTGTATA GTTCCTCAGC AGGACCTGCG GTCAGACATA AGTGCCTTAG AGCAATTCTT 
AGGATAATTT ATTTCGCGGA TGCCGAACTT CTAAAGGATG TCCTGAAAAA CCATGCTGTT 
TCAAGTCATA TTGCTTCCAT GCTGTCAAGC CAAGACCTGA AGATAGTAGT TGGAGCCCTT 
CAAATGGCAG AGATCTTAAT GCAGAAGTTA CCTGATATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGTGTAA TGCATCAAGT AAAACACTTA GCAGAATCTG AGTCATTGTT GACGAGTCCA 
CCAAAGGCAT GTACAAATGG GTCCGGATCC TTGGGGTCCA CACCATCGGT CAACAGTGGA 
ACAGCCACGG CTGCCACCAA TGCCTCGGCT GACCTGGGGT CCCCCAGCTT GCAGCACAGC 
AGAGACGATT CTCTAGACCT GAGCCCTCAA GGTCGATTAA GTGATGTTCT AAAGAGAAAA 
CGACTGCCAA AACGAGGATC AAGAAGGCCA AAGTATTCAC CTCCGAGAGA TGATGACAAA 
GTAGACAATC AAGCTAAAAG CCCCACCACT ACTCAGTCAC CTAAGTCTTC TTTCTTGGCA 
AGCTTGAATC CAAAAACGTG GGGAAGGTTA AGCGCACAGT CTAACAGCAA CAACATTGAA 
CCAGCACGAA CTGCGGGAGT TAGTGGTCTT GCCAGGGCCG CCTCAAAGGA CACCATCTCC 
AATAATAGAG AAAAAATTAA AGGTTGGATT AAAGAGCAGG CACATAAATT TGTAGAGCGT 
TACTTCAGTT CTGAGAACAT GGATGGAAGC AACCCCGCAT TGAATGTCCT TCAGAGACTT 
TGTGCTGCGA CTGAACAACT CAACCTCCAG GTGGATGGTG GAGCTGAGTG CCTTGTAGAA 
ATCCGTAGCA TAGTCTCAGA GTCTGATGTT TCATCGTTTG AAATCCAACA CAGTGGATTT 
GTGAAGCAGC TGTTGCTCTA TTTGACATCT AAAAGTGAAA AGGATGCTGT GAGCAGAGAG 
ATCAGATTAA AGAGATTCCT TCATGTATTT TTTTCTTCTC CACTTCCTGG AGAAGAGCCC 
ATTGAACGAG TAGAACCAGT GGGTAATGCA CCTTTGTTGG CATTAGTTCA CAAAATGAAC 
AACTGCCTCA GCCAGATGGA ACAATTTCCA GTCAAAGTGC ATGACTTTCC CAGTGGAAAT 
GGGACAGGAG GCAGCTTCTC TCTCAACAGA GGATCACAGG CTTTAAAATT TTTCAACACA 
CATCAGTTAA AATGCCAGTT ACAAAGACAT CCAGACTGTG CAAATGTGAA GCAGTGGAAG 
GGTGGACCTG TCAAGATTGA CCCCCTGGCT TTGGTGCAGG CTATTGAGAG ATATCTTGTA 
GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT GACGAAGACA GTGACGACGA TGGCTCTGAT 
GAGGAAATAG ACGAATCTCT GGCTGCTCAG TTCTTAAATT CCGGAAATGT GAGACACAGG 
CTACAGTTTT ACATCGGAGA GCATTTGCTA CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTCA GTATACAGGC TGAAGATGAA CGGGAGTCCA CGGATGACGA GAGCAATCCT 
CTAGGAAGAG CTGGTATTTG GACGAAAACT CATACGATAT GGTACAAACC TGTGCGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA GACAGCTCCA 
ACAAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGC TGTGGAATGA TGGAGTGTGC 
CCGTCCGTAT CAAATCCTTT AGAAGTTTAC CTCATACCCA CAGCACCCGA AAATATAACT 
TTTGAAGACC CGTCATTAGA TGTAATTCTT CTTTTAAGAG TTTTACACGC CGTCAGTAGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAGA TTATCCCAAC TAGTGAATTT 
ATTAACAGTA AGTTGACAGC AAAAGCAAAT AGGCAGCTTC AAGATCCTTT GGTAATCATG 
ACAGGAAACA TCCCTACATG GCTCACTGAA CTAGGAAAAA CCTGCCCATT TTTCTTCCCT 
TTTGATACCC GGCAAATGCT TTTCTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAGATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ACAGAAAAAA ACGTACTGTG AACAGAGAGG AGCTGCTGAA ACAAGCGGAG 
TCTGTGATGC AGGATCTTGG CAGCTCAAGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGCACAG GTCTTGGGCC TACACTGGAG TTTTATGCAC TTGTATCTCA GGAACTGCAG 
AGAGCTGACT TGGGTCTCTG GAGAGGGGAG GAAGTAACTC TTAGCAATCC AAAAGGAAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAAGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCTAAGC CAGCTCATAT CGCAAAGGTT AAAATGAAGT TTCGCTTCTT AGGAAAACTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTGGGCTT ACCTTTTTAT 
AAATGGATGC TCCGGCAAGA AACTTCGCTG ACGTCACATG ATTTGTTTGA CATTGACCCA 
GTTGTAGCCA GGTCAGTGTA TCACCTCGAA GACATCGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGCCTC CAATATGCAT TAGAAACCCT GACTATGAAC 
GGCTGCTCCG TGGAGGATCT GGGATTGGAT TTCACTCTGC CAGGGTTTCC TAACATTGAA 
CTAAAGAAAG GAGGAAAGGA TATACCGGTC ACCATCCACA ATTTGGAGGA GTATCTAAGA 
CTGGTTATAT TTTGGGCACT AAACGAAGGT GTTTCCAGGC AATTTGATTC ATTTAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTCCAGTACT TCTACCCAGA AGAACTGGAT 
CAGCTTCTGT GTGGTAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGC 
AGGCCAGATC ACGGTTACAC TCATGACAGT CGGGCTGTGA AGTTCTTGTT TGAGATTCTC 
AGTAGTTTTG ACAATGAACA GCAGAGGTTA TTTCTCCAGT TTGTGACAGG TAGCCCACGG 
TTGCCTGTTG GAGGCTTCCG GAGCTTGAAT CCACCTTTGA CCATTGTCCG GAAGACCTTC 
GAATCAACAG AGAACCCAGA TGACTTCCTA CCCTCTGTCA TGACCTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CCTTGAGATA ATGCGGGAAA AGCTGTTGAT GGCTGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047095.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047157.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

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CloneID	OBu05779
Clone ID Related Accession (Same CDS sequence)	XM_006047095.2 , XM_006047095.1
Accession Version	XM_006047095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ATCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GCTCTGATGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGAGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC GTCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCAAGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAGGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGCCTCCA ATATGCATTA GAAACCCTGA CTATGAACGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ACGAAGGTGT TTCCAGGCAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGGTT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TTGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047157.1
CDS	1..6078
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047095.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047095.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ATCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GCTCTGATGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGAGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC GTCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCAAGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAGGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGCCTCCA ATATGCATTA GAAACCCTGA CTATGAACGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ACGAAGGTGT TTCCAGGCAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGGTT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TTGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006047095.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047157.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OBu05779
Clone ID Related Accession (Same CDS sequence)	XM_006047095.2 , XM_006047095.1
Accession Version	XM_006047095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ATCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GCTCTGATGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGAGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC GTCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCAAGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAGGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGCCTCCA ATATGCATTA GAAACCCTGA CTATGAACGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ACGAAGGTGT TTCCAGGCAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGGTT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TTGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047157.1
CDS	50..6127
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006047095.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047095.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGCTTCTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CCCTGGCTTT GGTGCAGGCT ATTGAGAGAT ATCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA CGAAGACAGT GACGACGATG GCTCTGATGA GGAAATAGAC 
GAATCTCTGG CTGCTCAGTT CTTAAATTCC GGAAATGTGA GACACAGGCT ACAGTTTTAC 
ATCGGAGAGC ATTTGCTACC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTCAGT 
ATACAGGCTG AAGATGAACG GGAGTCCACG GATGACGAGA GCAATCCTCT AGGAAGAGCT 
GGTATTTGGA CGAAAACTCA TACGATATGG TACAAACCTG TGCGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAGA CAGCTCCAAC AAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGCTG TGGAATGATG GAGTGTGCCC GTCCGTATCA 
AATCCTTTAG AAGTTTACCT CATACCCACA GCACCCGAAA ATATAACTTT TGAAGACCCG 
TCATTAGATG TAATTCTTCT TTTAAGAGTT TTACACGCCG TCAGTAGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATCCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTGG TAATCATGAC AGGAAACATC 
CCTACATGGC TCACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TCTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AGATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAC 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTGATGCAG 
GATCTTGGCA GCTCAAGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGCACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTGCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGGGAGGA AGTAACTCTT AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA AGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCTCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAACTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTGGGCTTAC CTTTTTATAA ATGGATGCTC 
CGGCAAGAAA CTTCGCTGAC GTCACATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGG 
TCAGTGTATC ACCTCGAAGA CATCGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGCCTCCA ATATGCATTA GAAACCCTGA CTATGAACGG CTGCTCCGTG 
GAGGATCTGG GATTGGATTT CACTCTGCCA GGGTTTCCTA ACATTGAACT AAAGAAAGGA 
GGAAAGGATA TACCGGTCAC CATCCACAAT TTGGAGGAGT ATCTAAGACT GGTTATATTT 
TGGGCACTAA ACGAAGGTGT TTCCAGGCAA TTTGATTCAT TTAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT CCAGTACTTC TACCCAGAAG AACTGGATCA GCTTCTGTGT 
GGTAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGCAG GCCAGATCAC 
GGTTACACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAC 
AATGAACAGC AGAGGTTATT TCTCCAGTTT GTGACAGGTA GCCCACGGTT GCCTGTTGGA 
GGCTTCCGGA GCTTGAATCC ACCTTTGACC ATTGTCCGGA AGACCTTCGA ATCAACAGAG 
AACCCAGATG ACTTCCTACC CTCTGTCATG ACCTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCC TTGAGATAAT GCGGGAAAAG CTGTTGATGG CTGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278388.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134173.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OBu56070
Clone ID Related Accession (Same CDS sequence)	XM_025278388.1
Accession Version	XM_025278388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTGTCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACGAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACACCATC GGTCAACAGT 
GGAACAGCCA CGGCTGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCCG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CGACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AGCTGTTGCT CTATTTGACA TCTAAAAGTG AAAAGGATGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATATCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGCTCT 
GATGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG AGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCGTCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA AGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ATGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGC CTCCAATATG CATTAGAAAC CCTGACTATG 
AACGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAACGAA GGTGTTTCCA GGCAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTTGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134173.1
CDS	180..6392
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQSFTDIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278388.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278388.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AGCTTTACAG ACATTGCAGA AACGCTTCAC TTTCTCCTGT GTGGTGCCTC CAATGGAAGT 
TGTCAGGAGC AGATTGATCT TGTTCCACGA AGTCCTCAAG AACTGTATGA ACTGACATCT 
CTGATTTGTG AACTTATGCC ATGTTTACCA AAGGAAGGCA TTTTTGCAGT TGATACCATG 
CTGAAGAAGG GCAATGCACA GAACACGGAT GGGGCCATAT GGCAGTGGCG GGATGACCGG 
GGCCTCTGGC ACCCCTATAA CAGGATAGAC AGCCGGATCA TTGAGGCAGC CCATCAGGTC 
GGTGAGGATG AGATAAGCTT GTCCACTCTG GGACGAGTTT ATACTATTGA TTTTAATTCT 
ATGCAGCAAA TCAATGAGGA CACGGGAACA GCACGTGCCA TTCAGAGAAA ACCTAACCCC 
TTAGCCAATA CTAACACTAG TGGATATTCA GAGTTAAAGA AGGATGATGC TCGAGCACAG 
CTTATGAAAG AGGATCCGGA ACTGGCTAAG TCTTTTATTA AGACATTATT TGGTGTTCTT 
TATGAAGTGT ATAGTTCCTC AGCAGGACCT GCGGTCAGAC ATAAGTGCCT TAGAGCAATT 
CTTAGGATAA TTTATTTCGC GGATGCCGAA CTTCTAAAGG ATGTCCTGAA AAACCATGCT 
GTTTCAAGTC ATATTGCTTC CATGCTGTCA AGCCAAGACC TGAAGATAGT AGTTGGAGCC 
CTTCAAATGG CAGAGATCTT AATGCAGAAG TTACCTGATA TTTTTAGTGT TTACTTCAGA 
AGAGAAGGTG TAATGCATCA AGTAAAACAC TTAGCAGAAT CTGAGTCATT GTTGACGAGT 
CCACCAAAGG CATGTACAAA TGGGTCCGGA TCCTTGGGGT CCACACCATC GGTCAACAGT 
GGAACAGCCA CGGCTGCCAC CAATGCCTCG GCTGACCTGG GGTCCCCCAG CTTGCAGCAC 
AGCAGAGACG ATTCTCTAGA CCTGAGCCCT CAAGGTCGAT TAAGTGATGT TCTAAAGAGA 
AAACGACTGC CAAAACGAGG ATCAAGAAGG CCAAAGTATT CACCTCCGAG AGATGATGAC 
AAAGTAGACA ATCAAGCTAA AAGCCCCACC ACTACTCAGT CACCTAAGTC TTCTTTCTTG 
GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG TTAAGCGCAC AGTCTAACAG CAACAACATT 
GAACCAGCAC GAACTGCGGG AGTTAGTGGT CTTGCCAGGG CCGCCTCAAA GGACACCATC 
TCCAATAATA GAGAAAAAAT TAAAGGTTGG ATTAAAGAGC AGGCACATAA ATTTGTAGAG 
CGTTACTTCA GTTCTGAGAA CATGGATGGA AGCAACCCCG CATTGAATGT CCTTCAGAGA 
CTTTGTGCTG CGACTGAACA ACTCAACCTC CAGGTGGATG GTGGAGCTGA GTGCCTTGTA 
GAAATCCGTA GCATAGTCTC AGAGTCTGAT GTTTCATCGT TTGAAATCCA ACACAGTGGA 
TTTGTGAAGC AGCTGTTGCT CTATTTGACA TCTAAAAGTG AAAAGGATGC TGTGAGCAGA 
GAGATCAGAT TAAAGAGATT CCTTCATGTA TTTTTTTCTT CTCCACTTCC TGGAGAAGAG 
CCCATTGAAC GAGTAGAACC AGTGGGTAAT GCACCTTTGT TGGCATTAGT TCACAAAATG 
AACAACTGCC TCAGCCAGAT GGAACAATTT CCAGTCAAAG TGCATGACTT TCCCAGTGGA 
AATGGGACAG GAGGCAGCTT CTCTCTCAAC AGAGGATCAC AGGCTTTAAA ATTTTTCAAC 
ACACATCAGT TAAAATGCCA GTTACAAAGA CATCCAGACT GTGCAAATGT GAAGCAGTGG 
AAGGGTGGAC CTGTCAAGAT TGACCCCCTG GCTTTGGTGC AGGCTATTGA GAGATATCTT 
GTAGTTAGAG GGTATGGAAG AGTAAGAGAA GATGACGAAG ACAGTGACGA CGATGGCTCT 
GATGAGGAAA TAGACGAATC TCTGGCTGCT CAGTTCTTAA ATTCCGGAAA TGTGAGACAC 
AGGCTACAGT TTTACATCGG AGAGCATTTG CTACCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TCAGTATACA GGCTGAAGAT GAACGGGAGT CCACGGATGA CGAGAGCAAT 
CCTCTAGGAA GAGCTGGTAT TTGGACGAAA ACTCATACGA TATGGTACAA ACCTGTGCGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAGACAGCT 
CCAACAAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGCTGTGGAA TGATGGAGTG 
TGCCCGTCCG TATCAAATCC TTTAGAAGTT TACCTCATAC CCACAGCACC CGAAAATATA 
ACTTTTGAAG ACCCGTCATT AGATGTAATT CTTCTTTTAA GAGTTTTACA CGCCGTCAGT 
AGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AGATTATCCC AACTAGTGAA 
TTTATTAACA GTAAGTTGAC AGCAAAAGCA AATAGGCAGC TTCAAGATCC TTTGGTAATC 
ATGACAGGAA ACATCCCTAC ATGGCTCACT GAACTAGGAA AAACCTGCCC ATTTTTCTTC 
CCTTTTGATA CCCGGCAAAT GCTTTTCTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAGATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGACAGAAA AAAACGTACT GTGAACAGAG AGGAGCTGCT GAAACAAGCG 
GAGTCTGTGA TGCAGGATCT TGGCAGCTCA AGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGCA CAGGTCTTGG GCCTACACTG GAGTTTTATG CACTTGTATC TCAGGAACTG 
CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCTA AGCCAGCTCA TATCGCAAAG GTTAAAATGA AGTTTCGCTT CTTAGGAAAA 
CTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTGGG CTTACCTTTT 
TATAAATGGA TGCTCCGGCA AGAAACTTCG CTGACGTCAC ATGATTTGTT TGACATTGAC 
CCAGTTGTAG CCAGGTCAGT GTATCACCTC GAAGACATCG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGC CTCCAATATG CATTAGAAAC CCTGACTATG 
AACGGCTGCT CCGTGGAGGA TCTGGGATTG GATTTCACTC TGCCAGGGTT TCCTAACATT 
GAACTAAAGA AAGGAGGAAA GGATATACCG GTCACCATCC ACAATTTGGA GGAGTATCTA 
AGACTGGTTA TATTTTGGGC ACTAAACGAA GGTGTTTCCA GGCAATTTGA TTCATTTAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTCCAGT ACTTCTACCC AGAAGAACTG 
GATCAGCTTC TGTGTGGTAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGCAGGCCAG ATCACGGTTA CACTCATGAC AGTCGGGCTG TGAAGTTCTT GTTTGAGATT 
CTCAGTAGTT TTGACAATGA ACAGCAGAGG TTATTTCTCC AGTTTGTGAC AGGTAGCCCA 
CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG AATCCACCTT TGACCATTGT CCGGAAGACC 
TTCGAATCAA CAGAGAACCC AGATGACTTC CTACCCTCTG TCATGACCTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCCTTGAG ATAATGCGGG AAAAGCTGTT GATGGCTGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278389.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134174.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OBu56071
Clone ID Related Accession (Same CDS sequence)	XM_025278389.1
Accession Version	XM_025278389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC GAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACAC CATCGGTCAA CAGTGGAACA 
GCCACGGCTG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCCGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCGACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAGCTGT TGCTCTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA TCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG CTCTGATGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGAGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCG 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCAAGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAGGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGCCTCCAA TATGCATTAG AAACCCTGAC TATGAACGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA CGAAGGTGTT TCCAGGCAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGGTTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT TGAGATAATG CGGGAAAAGC TGTTGATGGC TGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134174.1
CDS	1..6207
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278389.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278389.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC GAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACAC CATCGGTCAA CAGTGGAACA 
GCCACGGCTG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCCGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCGACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAGCTGT TGCTCTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GCTTCTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC CCTGGCTTTG GTGCAGGCTA TTGAGAGATA TCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAC GAAGACAGTG ACGACGATGG CTCTGATGAG 
GAAATAGACG AATCTCTGGC TGCTCAGTTC TTAAATTCCG GAAATGTGAG ACACAGGCTA 
CAGTTTTACA TCGGAGAGCA TTTGCTACCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTCAGTA TACAGGCTGA AGATGAACGG GAGTCCACGG ATGACGAGAG CAATCCTCTA 
GGAAGAGCTG GTATTTGGAC GAAAACTCAT ACGATATGGT ACAAACCTGT GCGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAGAC AGCTCCAACA 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGCTGT GGAATGATGG AGTGTGCCCG 
TCCGTATCAA ATCCTTTAGA AGTTTACCTC ATACCCACAG CACCCGAAAA TATAACTTTT 
GAAGACCCGT CATTAGATGT AATTCTTCTT TTAAGAGTTT TACACGCCGT CAGTAGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TCCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTGGT AATCATGACA 
GGAAACATCC CTACATGGCT CACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT CTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA GATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGACA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTGATGCAGG ATCTTGGCAG CTCAAGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGCACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTGCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGGGAGGAA GTAACTCTTA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAA GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCTAAGCCAG CTCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAACTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TGGGCTTACC TTTTTATAAA 
TGGATGCTCC GGCAAGAAAC TTCGCTGACG TCACATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGGT CAGTGTATCA CCTCGAAGAC ATCGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGCCTCCAA TATGCATTAG AAACCCTGAC TATGAACGGC 
TGCTCCGTGG AGGATCTGGG ATTGGATTTC ACTCTGCCAG GGTTTCCTAA CATTGAACTA 
AAGAAAGGAG GAAAGGATAT ACCGGTCACC ATCCACAATT TGGAGGAGTA TCTAAGACTG 
GTTATATTTT GGGCACTAAA CGAAGGTGTT TCCAGGCAAT TTGATTCATT TAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTC CAGTACTTCT ACCCAGAAGA ACTGGATCAG 
CTTCTGTGTG GTAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGCAGG 
CCAGATCACG GTTACACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGACA ATGAACAGCA GAGGTTATTT CTCCAGTTTG TGACAGGTAG CCCACGGTTG 
CCTGTTGGAG GCTTCCGGAG CTTGAATCCA CCTTTGACCA TTGTCCGGAA GACCTTCGAA 
TCAACAGAGA ACCCAGATGA CTTCCTACCC TCTGTCATGA CCTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCCT TGAGATAATG CGGGAAAAGC TGTTGATGGC TGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278390.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134175.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OBu56072
Clone ID Related Accession (Same CDS sequence)	XM_025278390.1
Accession Version	XM_025278390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACGAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACACCAT CGGTCAACAG TGGAACAGCC 
ACGGCTGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCC GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCGACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAGCTGTTGC TCTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATATCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGCTC TGATGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GAGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCGTCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC AAGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAGGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG CCTCCAATAT GCATTAGAAA CCCTGACTAT GAACGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAACGA AGGTGTTTCC AGGCAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGGTTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTTGA GATAATGCGG GAAAAGCTGT TGATGGCTGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134175.1
CDS	1..6204
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278390.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278390.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACGAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACACCAT CGGTCAACAG TGGAACAGCC 
ACGGCTGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCC GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCGACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAGCTGTTGC TCTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGCT TCTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCCCT GGCTTTGGTG CAGGCTATTG AGAGATATCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGACGAA GACAGTGACG ACGATGGCTC TGATGAGGAA 
ATAGACGAAT CTCTGGCTGC TCAGTTCTTA AATTCCGGAA ATGTGAGACA CAGGCTACAG 
TTTTACATCG GAGAGCATTT GCTACCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTCAGTATAC AGGCTGAAGA TGAACGGGAG TCCACGGATG ACGAGAGCAA TCCTCTAGGA 
AGAGCTGGTA TTTGGACGAA AACTCATACG ATATGGTACA AACCTGTGCG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAGACAGC TCCAACAAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGCTGTGGA ATGATGGAGT GTGCCCGTCC 
GTATCAAATC CTTTAGAAGT TTACCTCATA CCCACAGCAC CCGAAAATAT AACTTTTGAA 
GACCCGTCAT TAGATGTAAT TCTTCTTTTA AGAGTTTTAC ACGCCGTCAG TAGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATCC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTGGTAAT CATGACAGGA 
AACATCCCTA CATGGCTCAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTCTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAGAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGACAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTG 
ATGCAGGATC TTGGCAGCTC AAGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGC 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT GCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG GGAGGAAGTA ACTCTTAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAAGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCTC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ACTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTGG GCTTACCTTT TTATAAATGG 
ATGCTCCGGC AAGAAACTTC GCTGACGTCA CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGGTCAG TGTATCACCT CGAAGACATC GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG CCTCCAATAT GCATTAGAAA CCCTGACTAT GAACGGCTGC 
TCCGTGGAGG ATCTGGGATT GGATTTCACT CTGCCAGGGT TTCCTAACAT TGAACTAAAG 
AAAGGAGGAA AGGATATACC GGTCACCATC CACAATTTGG AGGAGTATCT AAGACTGGTT 
ATATTTTGGG CACTAAACGA AGGTGTTTCC AGGCAATTTG ATTCATTTAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTCCAG TACTTCTACC CAGAAGAACT GGATCAGCTT 
CTGTGTGGTA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGCAGGCCA 
GATCACGGTT ACACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGACAATG AACAGCAGAG GTTATTTCTC CAGTTTGTGA CAGGTAGCCC ACGGTTGCCT 
GTTGGAGGCT TCCGGAGCTT GAATCCACCT TTGACCATTG TCCGGAAGAC CTTCGAATCA 
ACAGAGAACC CAGATGACTT CCTACCCTCT GTCATGACCT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCCTTGA GATAATGCGG GAAAAGCTGT TGATGGCTGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278392.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134177.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OBu56073
Clone ID Related Accession (Same CDS sequence)	XM_025278392.1
Accession Version	XM_025278392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC GAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACAC CATCGGTCAA CAGTGGAACA 
GCCACGGCTG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCCGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCGACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAGCTGT TGCTCTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134177.1
CDS	1..6192
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278392.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278392.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TCGCGGATGC CGAACTTCTA AAGGATGTCC TGAAAAACCA TGCTGTTTCA 
AGTCATATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCCCTTCAA 
ATGGCAGAGA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCTGAGT CATTGTTGAC GAGTCCACCA 
AAGGCATGTA CAAATGGGTC CGGATCCTTG GGGTCCACAC CATCGGTCAA CAGTGGAACA 
GCCACGGCTG CCACCAATGC CTCGGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GACGATTCTC TAGACCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGATCAAG AAGGCCAAAG TATTCACCTC CGAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AGTCTTCTTT CTTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCTA ACAGCAACAA CATTGAACCA 
GCACGAACTG CGGGAGTTAG TGGTCTTGCC AGGGCCGCCT CAAAGGACAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAA GAGCAGGCAC ATAAATTTGT AGAGCGTTAC 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCCGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCGACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCGTTTGAAA TCCAACACAG TGGATTTGTG 
AAGCAGCTGT TGCTCTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGA GATTCCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GAACGAGTAG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA AATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTGCATG ACTTTCCCAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCCCTGG CTTTGGTGCA GGCTATTGAG AGATATCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGACGAAGA CAGTGACGAC GATGGCTCTG ATGAGGAAAT AGACGAATCT 
CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT GTGAGACACA GGCTACAGTT TTACATCGGA 
GAGCATTTGC TACCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT CAGTATACAG 
GCTGAAGATG AACGGGAGTC CACGGATGAC GAGAGCAATC CTCTAGGAAG AGCTGGTATT 
TGGACGAAAA CTCATACGAT ATGGTACAAA CCTGTGCGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAGACAGCTC CAACAAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GCTGTGGAAT GATGGAGTGT GCCCGTCCGT ATCAAATCCT 
TTAGAAGTTT ACCTCATACC CACAGCACCC GAAAATATAA CTTTTGAAGA CCCGTCATTA 
GATGTAATTC TTCTTTTAAG AGTTTTACAC GCCGTCAGTA GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATCCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTGGTAATCA TGACAGGAAA CATCCCTACA 
TGGCTCACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTCTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAGATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGACAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTGAT GCAGGATCTT 
GGCAGCTCAA GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGCAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTGC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAAGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCTAA GCCAGCTCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAC TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTGGGC TTACCTTTTT ATAAATGGAT GCTCCGGCAA 
GAAACTTCGC TGACGTCACA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGGTCAGTG 
TATCACCTCG AAGACATCGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGCC TCCAATATGC ATTAGAAACC CTGACTATGA ACGGCTGCTC CGTGGAGGAT 
CTGGGATTGG ATTTCACTCT GCCAGGGTTT CCTAACATTG AACTAAAGAA AGGAGGAAAG 
GATATACCGG TCACCATCCA CAATTTGGAG GAGTATCTAA GACTGGTTAT ATTTTGGGCA 
CTAAACGAAG GTGTTTCCAG GCAATTTGAT TCATTTAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTCCAGTA CTTCTACCCA GAAGAACTGG ATCAGCTTCT GTGTGGTAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GCAGGCCAGA TCACGGTTAC 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGACAATGAA 
CAGCAGAGGT TATTTCTCCA GTTTGTGACA GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC 
CGGAGCTTGA ATCCACCTTT GACCATTGTC CGGAAGACCT TCGAATCAAC AGAGAACCCA 
GATGACTTCC TACCCTCTGT CATGACCTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCCTTGAGA TAATGCGGGA AAAGCTGTTG ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278394.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134179.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OBu56074
Clone ID Related Accession (Same CDS sequence)	XM_025278394.1
Accession Version	XM_025278394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACGAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACACCAT CGGTCAACAG TGGAACAGCC 
ACGGCTGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCC GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCGACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAGCTGTTGC TCTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134179.1
CDS	1..6189
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278394.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278394.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTCG CGGATGCCGA ACTTCTAAAG GATGTCCTGA AAAACCATGC TGTTTCAAGT 
CATATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC CCTTCAAATG 
GCAGAGATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCTGAGTCAT TGTTGACGAG TCCACCAAAG 
GCATGTACAA ATGGGTCCGG ATCCTTGGGG TCCACACCAT CGGTCAACAG TGGAACAGCC 
ACGGCTGCCA CCAATGCCTC GGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAC 
GATTCTCTAG ACCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GATCAAGAAG GCCAAAGTAT TCACCTCCGA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAGT CTTCTTTCTT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCTAACA GCAACAACAT TGAACCAGCA 
CGAACTGCGG GAGTTAGTGG TCTTGCCAGG GCCGCCTCAA AGGACACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAAGAG CAGGCACATA AATTTGTAGA GCGTTACTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCC GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCGACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCG TTTGAAATCC AACACAGTGG ATTTGTGAAG 
CAGCTGTTGC TCTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGAGAT TCCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGAA 
CGAGTAGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAAAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTGCATGACT TTCCCAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCCCTGGCTT TGGTGCAGGC TATTGAGAGA TATCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ACGAAGACAG TGACGACGAT GGCTCTGATG AGGAAATAGA CGAATCTCTG 
GCTGCTCAGT TCTTAAATTC CGGAAATGTG AGACACAGGC TACAGTTTTA CATCGGAGAG 
CATTTGCTAC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTCAG TATACAGGCT 
GAAGATGAAC GGGAGTCCAC GGATGACGAG AGCAATCCTC TAGGAAGAGC TGGTATTTGG 
ACGAAAACTC ATACGATATG GTACAAACCT GTGCGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAG ACAGCTCCAA CAAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGCT GTGGAATGAT GGAGTGTGCC CGTCCGTATC AAATCCTTTA 
GAAGTTTACC TCATACCCAC AGCACCCGAA AATATAACTT TTGAAGACCC GTCATTAGAT 
GTAATTCTTC TTTTAAGAGT TTTACACGCC GTCAGTAGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATCCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTG GTAATCATGA CAGGAAACAT CCCTACATGG 
CTCACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTCTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAGATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA CAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTGATGCA GGATCTTGGC 
AGCTCAAGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGCACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTGCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGGGAGG AAGTAACTCT TAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AAGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCTAAGCC AGCTCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAACTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTGGGCTTA CCTTTTTATA AATGGATGCT CCGGCAAGAA 
ACTTCGCTGA CGTCACATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG GTCAGTGTAT 
CACCTCGAAG ACATCGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGCCTCC AATATGCATT AGAAACCCTG ACTATGAACG GCTGCTCCGT GGAGGATCTG 
GGATTGGATT TCACTCTGCC AGGGTTTCCT AACATTGAAC TAAAGAAAGG AGGAAAGGAT 
ATACCGGTCA CCATCCACAA TTTGGAGGAG TATCTAAGAC TGGTTATATT TTGGGCACTA 
AACGAAGGTG TTTCCAGGCA ATTTGATTCA TTTAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TCCAGTACTT CTACCCAGAA GAACTGGATC AGCTTCTGTG TGGTAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGCA GGCCAGATCA CGGTTACACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA CAATGAACAG 
CAGAGGTTAT TTCTCCAGTT TGTGACAGGT AGCCCACGGT TGCCTGTTGG AGGCTTCCGG 
AGCTTGAATC CACCTTTGAC CATTGTCCGG AAGACCTTCG AATCAACAGA GAACCCAGAT 
GACTTCCTAC CCTCTGTCAT GACCTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
CTTGAGATAA TGCGGGAAAA GCTGTTGATG GCTGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278398.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134183.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

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CloneID	OBu56075
Clone ID Related Accession (Same CDS sequence)	XM_025278398.1
Accession Version	XM_025278398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT CGCGGATGCC 
GAACTTCTAA AGGATGTCCT GAAAAACCAT GCTGTTTCAA GTCATATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCCCTTCAAA TGGCAGAGAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCTGAGTC ATTGTTGACG AGTCCACCAA AGGCATGTAC AAATGGGTCC 
GGATCCTTGG GGTCCACACC ATCGGTCAAC AGTGGAACAG CCACGGCTGC CACCAATGCC 
TCGGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ACGATTCTCT AGACCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGATCAAGA 
AGGCCAAAGT ATTCACCTCC GAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA GTCTTCTTTC TTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCTAA CAGCAACAAC ATTGAACCAG CACGAACTGC GGGAGTTAGT 
GGTCTTGCCA GGGCCGCCTC AAAGGACACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAAG AGCAGGCACA TAAATTTGTA GAGCGTTACT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CCGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCGACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CGTTTGAAAT CCAACACAGT GGATTTGTGA AGCAGCTGTT GCTCTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGAG ATTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG AACGAGTAGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAA ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTGCATGA CTTTCCCAGT GGAAATGGGA CAGGAGGCAG CTTCTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAT CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGC TCTGATGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGAGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCGT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCAAGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAGGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACATGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGCCTCCAAT ATGCATTAGA AACCCTGACT ATGAACGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAC 
GAAGGTGTTT CCAGGCAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGGTTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTT 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134183.1
CDS	1..6126
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278398.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278398.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG CTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCC AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGCGAAT CCCCGCCTGC CGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GCGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCCGAC 
CCTGAAGGCA ACCAGGAGAC GGTGAACTCT TCAGCTGCAC GGACAGATGA GACTCCCCAA 
GGAGCAGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACTACCTC TGGGGAGAGC 
GAATCTGATG ACTCCGAGAT GGGACGGTTA CAAGCTCTGT TAGAGGCCAG GGGTCTTCCC 
CCTCACCTGT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG CGATGAAAGC 
CAGCAGCTCC AGGCCGTTAT TGAGATGTGT CAGTTACTGG TCATGGGGAA TGAGGAGACG 
CTGGGAGGGT TTCCTGTCAA GAGTGTGGTT CCAGCTTTGA TAACATTGCT GCAGATGGAA 
CATAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCAT CAGCTGTGGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC CGAACAGGCC TTGACAGCCT TGGAGATGTT ATCACGTAGA 
CACAGTAAAG CCATTCTCCA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAGTTC 
TTCAGCATAA ATGCCCAAAG AAACGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCGGAT TCCCTCCCAT TGCTTACCCA AAGGCTAACC 
CACCAGGACA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT AACAAATGTT 
CAACAACTGT TGGTAGTAAC TCCACCAATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAATTGT CCAACTTTAG CAGTTCAACT TATGAAGCAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAG 
CAGATTGATC TTGTTCCACG AAGTCCTCAA GAACTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAGGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGCAATGCAC AGAACACGGA TGGGGCCATA TGGCAGTGGC GGGATGACCG GGGCCTCTGG 
CACCCCTATA ACAGGATAGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT CGCGGATGCC 
GAACTTCTAA AGGATGTCCT GAAAAACCAT GCTGTTTCAA GTCATATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCCCTTCAAA TGGCAGAGAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCTGAGTC ATTGTTGACG AGTCCACCAA AGGCATGTAC AAATGGGTCC 
GGATCCTTGG GGTCCACACC ATCGGTCAAC AGTGGAACAG CCACGGCTGC CACCAATGCC 
TCGGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ACGATTCTCT AGACCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGATCAAGA 
AGGCCAAAGT ATTCACCTCC GAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA GTCTTCTTTC TTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCTAA CAGCAACAAC ATTGAACCAG CACGAACTGC GGGAGTTAGT 
GGTCTTGCCA GGGCCGCCTC AAAGGACACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAAG AGCAGGCACA TAAATTTGTA GAGCGTTACT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CCGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCGACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CGTTTGAAAT CCAACACAGT GGATTTGTGA AGCAGCTGTT GCTCTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGAG ATTCCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG AACGAGTAGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAA ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTGCATGA CTTTCCCAGT GGAAATGGGA CAGGAGGCAG CTTCTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCC 
CTGGCTTTGG TGCAGGCTAT TGAGAGATAT CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGACG AAGACAGTGA CGACGATGGC TCTGATGAGG AAATAGACGA ATCTCTGGCT 
GCTCAGTTCT TAAATTCCGG AAATGTGAGA CACAGGCTAC AGTTTTACAT CGGAGAGCAT 
TTGCTACCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTCAGTAT ACAGGCTGAA 
GATGAACGGG AGTCCACGGA TGACGAGAGC AATCCTCTAG GAAGAGCTGG TATTTGGACG 
AAAACTCATA CGATATGGTA CAAACCTGTG CGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAGACA GCTCCAACAA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGCTGTG GAATGATGGA GTGTGCCCGT CCGTATCAAA TCCTTTAGAA 
GTTTACCTCA TACCCACAGC ACCCGAAAAT ATAACTTTTG AAGACCCGTC ATTAGATGTA 
ATTCTTCTTT TAAGAGTTTT ACACGCCGTC AGTAGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT CCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTGGTA ATCATGACAG GAAACATCCC TACATGGCTC 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTC 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAG 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGACAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TGATGCAGGA TCTTGGCAGC 
TCAAGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GCACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTGCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGGGAGGAAG TAACTCTTAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAAG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CTAAGCCAGC TCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAACTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT GGGCTTACCT TTTTATAAAT GGATGCTCCG GCAAGAAACT 
TCGCTGACGT CACATGATTT GTTTGACATT GACCCAGTTG TAGCCAGGTC AGTGTATCAC 
CTCGAAGACA TCGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGCCTCCAAT ATGCATTAGA AACCCTGACT ATGAACGGCT GCTCCGTGGA GGATCTGGGA 
TTGGATTTCA CTCTGCCAGG GTTTCCTAAC ATTGAACTAA AGAAAGGAGG AAAGGATATA 
CCGGTCACCA TCCACAATTT GGAGGAGTAT CTAAGACTGG TTATATTTTG GGCACTAAAC 
GAAGGTGTTT CCAGGCAATT TGATTCATTT AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTCC AGTACTTCTA CCCAGAAGAA CTGGATCAGC TTCTGTGTGG TAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGCAGGC CAGATCACGG TTACACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGACAA TGAACAGCAG 
AGGTTATTTC TCCAGTTTGT GACAGGTAGC CCACGGTTGC CTGTTGGAGG CTTCCGGAGC 
TTGAATCCAC CTTTGACCAT TGTCCGGAAG ACCTTCGAAT CAACAGAGAA CCCAGATGAC 
TTCCTACCCT CTGTCATGAC CTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCCTT 
GAGATAATGC GGGAAAAGCT GTTGATGGCT GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278399.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134184.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

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CloneID	OBu56076
Clone ID Related Accession (Same CDS sequence)	XM_025278399.1
Accession Version	XM_025278399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATATCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGCTCTG ATGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGA GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCGTCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAA GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGCC TCCAATATGC ATTAGAAACC 
CTGACTATGA ACGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAACGAAG GTGTTTCCAG GCAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTTGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134184.1
CDS	1..6102
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278399.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278399.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCGTCA 
TCTGTTGTCA TAGTCCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAAGCAG 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CCAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACGAG TAAGCCACAT AGTAAGTCAA AGAAGAGACA CTTAGACCAG 
GAGCCACAAC TGAAATCTGC ACCATCTCCA TCCACGAGCA AGGCTCATAC CAGAAAGAGT 
GGGGCCGCTG CTGGTTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTATAAAG 
AGTGCTTCTG TGTCTGAAGC CACTGGTGCC GAAGAGAGAT CTGCAAAACC TACCAAGCTG 
GCTTCAAAAT CGGCCGCCTC AGCCAAAGCT GGGTGTAGCA CCATCACCGA CTCGTCGTCC 
GCTGCCTCGA CCTCCTCCTC GTCCTCTGCC GTCGCCTCGG CCTCCTCTGC TGTTCCACCA 
GGTGCCAGGG TGAAACAAGG AAAAGACCAG AACAAGGCCA GGCGTTCCCG CTCAGCATCC 
AGTCCCAGCC CCAGAAGAAG CAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCCAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGCG AATCCCCGCC TGCCGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGCGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCC GACCCTGAAG GCAACCAGGA GACGGTGAAC 
TCTTCAGCTG CACGGACAGA TGAGACTCCC CAAGGAGCAG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACTAC CTCTGGGGAG AGCGAATCTG ATGACTCCGA GATGGGACGG 
TTACAAGCTC TGTTAGAGGC CAGGGGTCTT CCCCCTCACC TGTTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGCGATGAA AGCCAGCAGC TCCAGGCCGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG GAATGAGGAG ACGCTGGGAG GGTTTCCTGT CAAGAGTGTG 
GTTCCAGCTT TGATAACATT GCTGCAGATG GAACATAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CATCAGCTGT GGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCCGAACAG 
GCCTTGACAG CCTTGGAGAT GTTATCACGT AGACACAGTA AAGCCATTCT CCAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAG TTCTTCAGCA TAAATGCCCA AAGAAACGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCG 
GATTCCCTCC CATTGCTTAC CCAAAGGCTA ACCCACCAGG ACAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTAACAAAT GTTCAACAAC TGTTGGTAGT AACTCCACCA 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAT 
TGTCCAACTT TAGCAGTTCA ACTTATGAAG CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAGCAGATTG ATCTTGTTCC ACGAAGTCCT 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAGGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGCAATG CACAGAACAC GGATGGGGCC 
ATATGGCAGT GGCGGGATGA CCGGGGCCTC TGGCACCCCT ATAACAGGAT AGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TCGCGGATGC CGAACTTCTA 
AAGGATGTCC TGAAAAACCA TGCTGTTTCA AGTCATATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCCCTTCAA ATGGCAGAGA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCTGAGT CATTGTTGAC GAGTCCACCA AAGGCATGTA CAAATGGGTC CGGATCCTTG 
GGGTCCACAC CATCGGTCAA CAGTGGAACA GCCACGGCTG CCACCAATGC CTCGGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GACGATTCTC TAGACCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGATCAAG AAGGCCAAAG 
TATTCACCTC CGAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AGTCTTCTTT CTTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCTA ACAGCAACAA CATTGAACCA GCACGAACTG CGGGAGTTAG TGGTCTTGCC 
AGGGCCGCCT CAAAGGACAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAA 
GAGCAGGCAC ATAAATTTGT AGAGCGTTAC TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCCGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCGACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCGTTTGAAA TCCAACACAG TGGATTTGTG AAGCAGCTGT TGCTCTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGA GATTCCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GAACGAGTAG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA AATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTGCATG ACTTTCCCAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCCCTGG CTTTGGTGCA GGCTATTGAG 
AGATATCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGACGAAGA CAGTGACGAC 
GATGGCTCTG ATGAGGAAAT AGACGAATCT CTGGCTGCTC AGTTCTTAAA TTCCGGAAAT 
GTGAGACACA GGCTACAGTT TTACATCGGA GAGCATTTGC TACCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT CAGTATACAG GCTGAAGATG AACGGGAGTC CACGGATGAC 
GAGAGCAATC CTCTAGGAAG AGCTGGTATT TGGACGAAAA CTCATACGAT ATGGTACAAA 
CCTGTGCGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAGACAGCTC CAACAAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GCTGTGGAAT 
GATGGAGTGT GCCCGTCCGT ATCAAATCCT TTAGAAGTTT ACCTCATACC CACAGCACCC 
GAAAATATAA CTTTTGAAGA CCCGTCATTA GATGTAATTC TTCTTTTAAG AGTTTTACAC 
GCCGTCAGTA GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATCCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTGGTAATCA TGACAGGAAA CATCCCTACA TGGCTCACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTCTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAGATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGACAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTGAT GCAGGATCTT GGCAGCTCAA GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGCAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTGC AGAGAGCTGA CTTGGGTCTC TGGAGAGGGG AGGAAGTAAC TCTTAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAAGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCTAA GCCAGCTCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAC TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTGGGC 
TTACCTTTTT ATAAATGGAT GCTCCGGCAA GAAACTTCGC TGACGTCACA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGGTCAGTG TATCACCTCG AAGACATCGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGCC TCCAATATGC ATTAGAAACC 
CTGACTATGA ACGGCTGCTC CGTGGAGGAT CTGGGATTGG ATTTCACTCT GCCAGGGTTT 
CCTAACATTG AACTAAAGAA AGGAGGAAAG GATATACCGG TCACCATCCA CAATTTGGAG 
GAGTATCTAA GACTGGTTAT ATTTTGGGCA CTAAACGAAG GTGTTTCCAG GCAATTTGAT 
TCATTTAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTCCAGTA CTTCTACCCA 
GAAGAACTGG ATCAGCTTCT GTGTGGTAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GCAGGCCAGA TCACGGTTAC ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGACAATGAA CAGCAGAGGT TATTTCTCCA GTTTGTGACA 
GGTAGCCCAC GGTTGCCTGT TGGAGGCTTC CGGAGCTTGA ATCCACCTTT GACCATTGTC 
CGGAAGACCT TCGAATCAAC AGAGAACCCA GATGACTTCC TACCCTCTGT CATGACCTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCCTTGAGA TAATGCGGGA AAAGCTGTTG 
ATGGCTGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025278401.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025134186.1 Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

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CloneID	OBu56077
Clone ID Related Accession (Same CDS sequence)	XM_025278401.1
Accession Version	XM_025278401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025134186.1
CDS	1..6063
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSVVIVPQPEDPDRANTSEKQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETSKPHSKSKKRHLDQEPQLKSAPSPSTSKAHTRKSGAAAGSRSQKRKRTESSCIKSASVSEATGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSAVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPEGNQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTPSVNSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGSRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIERVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWNDGVCPSVSNPLEVYLIPTAPENITFEDPSLDVILLLRVLHAVSRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSLEIMREKLLMAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025278401.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025278401.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC GTCATCTGTT 
GTCATAGTCC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAA GCAGAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCCAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CGAGTAAGCC ACATAGTAAG TCAAAGAAGA GACACTTAGA CCAGGAGCCA 
CAACTGAAAT CTGCACCATC TCCATCCACG AGCAAGGCTC ATACCAGAAA GAGTGGGGCC 
GCTGCTGGTT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTAT AAAGAGTGCT 
TCTGTGTCTG AAGCCACTGG TGCCGAAGAG AGATCTGCAA AACCTACCAA GCTGGCTTCA 
AAATCGGCCG CCTCAGCCAA AGCTGGGTGT AGCACCATCA CCGACTCGTC GTCCGCTGCC 
TCGACCTCCT CCTCGTCCTC TGCCGTCGCC TCGGCCTCCT CTGCTGTTCC ACCAGGTGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAACAAG GCCAGGCGTT CCCGCTCAGC ATCCAGTCCC 
AGCCCCAGAA GAAGCAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGCTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCCAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGC GAATCCCCGC CTGCCGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGCGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC CGACCCTGAA 
GGCAACCAGG AGACGGTGAA CTCTTCAGCT GCACGGACAG ATGAGACTCC CCAAGGAGCA 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACTA CCTCTGGGGA GAGCGAATCT 
GATGACTCCG AGATGGGACG GTTACAAGCT CTGTTAGAGG CCAGGGGTCT TCCCCCTCAC 
CTGTTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGCGATGA AAGCCAGCAG 
CTCCAGGCCG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GGAATGAGGA GACGCTGGGA 
GGGTTTCCTG TCAAGAGTGT GGTTCCAGCT TTGATAACAT TGCTGCAGAT GGAACATAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCATCAGCTG TGGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCCGAACA GGCCTTGACA GCCTTGGAGA TGTTATCACG TAGACACAGT 
AAAGCCATTC TCCAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA GTTCTTCAGC 
ATAAATGCCC AAAGAAACGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC GGATTCCCTC CCATTGCTTA CCCAAAGGCT AACCCACCAG 
GACAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTAACAAA TGTTCAACAA 
CTGTTGGTAG TAACTCCACC AATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA TTGTCCAACT TTAGCAGTTC AACTTATGAA GCAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAGCAGATT 
GATCTTGTTC CACGAAGTCC TCAAGAACTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAGGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGCAAT 
GCACAGAACA CGGATGGGGC CATATGGCAG TGGCGGGATG ACCGGGGCCT CTGGCACCCC 
TATAACAGGA TAGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTCGCGGATG CCGAACTTCT AAAGGATGTC CTGAAAAACC ATGCTGTTTC AAGTCATATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCCCTTCA AATGGCAGAG 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCTGAG TCATTGTTGA CGAGTCCACC AAAGGCATGT 
ACAAATGGGT CCGGATCCTT GGGGTCCACA CCATCGGTCA ACAGTGGAAC AGCCACGGCT 
GCCACCAATG CCTCGGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGACGATTCT 
CTAGACCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGATCAA GAAGGCCAAA GTATTCACCT CCGAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAGTCTTCTT TCTTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCT AACAGCAACA ACATTGAACC AGCACGAACT 
GCGGGAGTTA GTGGTCTTGC CAGGGCCGCC TCAAAGGACA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA AGAGCAGGCA CATAAATTTG TAGAGCGTTA CTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCCGCATTG AATGTCCTTC AGAGACTTTG TGCTGCGACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCGTTTGAA ATCCAACACA GTGGATTTGT GAAGCAGCTG 
TTGCTCTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
AGATTCCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGAACGAGTA 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AAATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTGCAT GACTTTCCCA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCCCTG 
GCTTTGGTGC AGGCTATTGA GAGATATCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGACGAAG ACAGTGACGA CGATGGCTCT GATGAGGAAA TAGACGAATC TCTGGCTGCT 
CAGTTCTTAA ATTCCGGAAA TGTGAGACAC AGGCTACAGT TTTACATCGG AGAGCATTTG 
CTACCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TCAGTATACA GGCTGAAGAT 
GAACGGGAGT CCACGGATGA CGAGAGCAAT CCTCTAGGAA GAGCTGGTAT TTGGACGAAA 
ACTCATACGA TATGGTACAA ACCTGTGCGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAGACAGCT CCAACAAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGCTGTGGAA TGATGGAGTG TGCCCGTCCG TATCAAATCC TTTAGAAGTT 
TACCTCATAC CCACAGCACC CGAAAATATA ACTTTTGAAG ACCCGTCATT AGATGTAATT 
CTTCTTTTAA GAGTTTTACA CGCCGTCAGT AGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATCCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTGGTAATC ATGACAGGAA ACATCCCTAC ATGGCTCACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTCTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAGATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGACAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTGA TGCAGGATCT TGGCAGCTCA 
AGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGCA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTG CAGAGAGCTG ACTTGGGTCT CTGGAGAGGG 
GAGGAAGTAA CTCTTAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAAGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCTA AGCCAGCTCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA CTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTGGG CTTACCTTTT TATAAATGGA TGCTCCGGCA AGAAACTTCG 
CTGACGTCAC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGGTCAGT GTATCACCTC 
GAAGACATCG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGC 
CTCCAATATG CATTAGAAAC CCTGACTATG AACGGCTGCT CCGTGGAGGA TCTGGGATTG 
GATTTCACTC TGCCAGGGTT TCCTAACATT GAACTAAAGA AAGGAGGAAA GGATATACCG 
GTCACCATCC ACAATTTGGA GGAGTATCTA AGACTGGTTA TATTTTGGGC ACTAAACGAA 
GGTGTTTCCA GGCAATTTGA TTCATTTAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTCCAGT ACTTCTACCC AGAAGAACTG GATCAGCTTC TGTGTGGTAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGCAGGCCAG ATCACGGTTA CACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGACAATGA ACAGCAGAGG 
TTATTTCTCC AGTTTGTGAC AGGTAGCCCA CGGTTGCCTG TTGGAGGCTT CCGGAGCTTG 
AATCCACCTT TGACCATTGT CCGGAAGACC TTCGAATCAA CAGAGAACCC AGATGACTTC 
CTACCCTCTG TCATGACCTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCCTTGAG 
ATAATGCGGG AAAAGCTGTT GATGGCTGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIP12
Entrez Gene ID	102406749
Full Name	thyroid hormone receptor interactor 12
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIP12 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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