VPS13B cDNA ORF clone, Bubalus bubalis(water buffalo)

The following VPS13B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS13B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu07159 XM_025264779.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBu49008 XM_025264778.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu07155 XM_025264777.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu49006 XM_025264775.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu49007 XM_025264776.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu49005 XM_025264774.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu12536 XM_006055235.1
Latest version!
Bubalus bubalis vacuolar protein sorting-associated protein 13B-like (LOC102406246), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu48998 XM_025264764.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu48998 XM_025264765.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu48998 XM_025264766.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu48998 XM_025264767.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu48999 XM_025264768.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu49000 XM_025264769.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu49001 XM_025264770.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu49002 XM_025264771.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu49003 XM_025264772.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu49004 XM_025264773.1
Latest version!
Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu07159
    Clone ID Related Accession (Same CDS sequence) XM_025264779.1
    Accession Version XM_025264779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GATAAGAAAA AAGCCAGTCG TATACAGATT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120564.1
    CDS1..1686
    Translation

    Target ORF information:

    RefSeq Version XM_025264779.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GATAAGAAAA AAGCCAGTCG TATACAGATT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49008
    Clone ID Related Accession (Same CDS sequence) XM_025264778.1
    Accession Version XM_025264778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGATGTAT TCCTAGTGTC TAGAACAATA TCCCACAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120563.1
    CDS1..2862
    Translation

    Target ORF information:

    RefSeq Version XM_025264778.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGATGTAT TCCTAGTGTC TAGAACAATA TCCCACAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu07155
    Clone ID Related Accession (Same CDS sequence) XM_025264777.1
    Accession Version XM_025264777.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTTCTA CTCTTCAGGA CGCTGCCTTT GCTGCTCATC TAGCTTCTGT GTATATTTAT
    GGGCACCAAG TGAAGAGCTG TACTGCTCCA TATTATAGGA ATACACTGCA AGCTCTGCGT
    TCAGAGGCAA GAGACATATT GGATGGACAC AGGCTTTGTC AGTTTTTCCT GAGTGGAGTT
    GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120562.1
    CDS1..3006
    Translation

    Target ORF information:

    RefSeq Version XM_025264777.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264777.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTTCTA CTCTTCAGGA CGCTGCCTTT GCTGCTCATC TAGCTTCTGT GTATATTTAT
    GGGCACCAAG TGAAGAGCTG TACTGCTCCA TATTATAGGA ATACACTGCA AGCTCTGCGT
    TCAGAGGCAA GAGACATATT GGATGGACAC AGGCTTTGTC AGTTTTTCCT GAGTGGAGTT
    GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49006
    Clone ID Related Accession (Same CDS sequence) XM_025264775.1
    Accession Version XM_025264775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAAAAAAT TGAAGAGAAC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120560.1
    CDS1..4176
    Translation

    Target ORF information:

    RefSeq Version XM_025264775.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAAAAAAT TGAAGAGAAC AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49007
    Clone ID Related Accession (Same CDS sequence) XM_025264776.1
    Accession Version XM_025264776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAACAACT GCAAAGCTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120561.1
    CDS1..4173
    Translation

    Target ORF information:

    RefSeq Version XM_025264776.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAACAACT GCAAAGCTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49005
    Clone ID Related Accession (Same CDS sequence) XM_025264774.1
    Accession Version XM_025264774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120559.1
    CDS1..7311
    Translation

    Target ORF information:

    RefSeq Version XM_025264774.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu12536
    Clone ID Related Accession (Same CDS sequence) XM_006055235.1
    Accession Version XM_006055235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784677.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGATCGTTT CCAAACGAAC GGCCCAGAGC CGACAGTGCG TGCTGCTGGT GGAGCAGGCA 
    GTGGAGAGTC CAAGAGCTCT ACCCACAGGA GGCGTCTGCA GGAATGGAGA TTCTGTACAA
    GCTGTTGATG ATTCACCATT CTCAGATTCT GTTACCTTGG AACAGACTAC AAGTAACATT
    GGTGGATCCA GTGGTCGTGT TAGTCTGTGG ATGCAGTGGG TGCTCCCCAA AATTACTGTA
    AAGCTCTTTG CTCCAGATCC TGACAGTAAA GGCATAGAAC TTTGTATGGT CAGTGAACTA
    GAAGATCTCA GTGCTTCAAT AGATGTCCAG GATGTGTACA CTAAAGTGAA ATGTAAAATA
    GAAAGTTTCA ATATTGATCA CTATAGAAGC AACCTTGGGG AAGAGTGTTG GTCTCTGGGG
    CAATGTGGAG GTGTCTTCCT TTCCTGTACT GACAAGCTGA ACAGACGCAC CTTGTTGGTT
    CGACCCATCA GCAAGCAGGA CCCTTTCAGT AATTGCTCTG GCTTCTTTCC TTCTACAACT
    GCAAAGCTTC TAGATGGCTC TCATCAGCAG CATGGATTTC TGTCTTTGAC ATACACAAAA
    GCTGTAACAA AAAATGTTCG CCACAAGTTA ACATCAAGAA ATGAGCGAAG AAGTTTTCAT
    AAGTTATCTG AAGGTTTAAC AGATGGCTCC CCTCATTTTC TTCATGAAAT ACTTCTTTCA
    GCACAAGCCT TTGATATTGT CCTTTGTTTT CCTTTACTTA ATGCCATTGC AAGTATATTT
    CAAGCAAAAC TACCAAGGAC CCAAAAAGAG AAAAGAAAAT CTCCTGGTCA GCCCATGAGG
    ACCCATACAC TGACTTCACG CAATTTACCT TTGATTTATA TCAACACAAG CGTAATCAGA
    ATTTTTGTTC CAAAAACAGA AGAAATGCAG CCAAGTATTG AAGTTAATCA GACAGCAAAG
    GAAGATACCA TGGTTCTGAA GATTGGCTCT GTTGCTATGG CTCCCCAGGC TGACAATCCC
    CTTGGCAGAT CCGTCCTCAG GAAAGATATT TACCAAAGAG CCTTGAATTT AGGAATTCTT
    CGAGACCCTG GGTCAGAGAT TGAAGACCGA CAATACCAAA TAGATCTGCA GTCGATCAAT
    ATTGGTACTG CTCAGTGGGA TCAACTGAAA CCAGAAAAGG AAAATGGCAC AGGAGGAGTG
    CTAACAGAGA CTGAAAGAAA TTCGCAGAAT CCAGCCCTTG AGTGGAATAT GGCCAGCAGC
    ATACGGCGGC ATCAAGAAAG AAGAGCAATT TTGACTCCCA TTTTAACAGA TTTTTCTGTA
    CGAATAACTG GAGCACCTGC CATCATTTTC ACCAAAATAA TTTCTCCGGA AAATATGCAC
    ACTGAGGAGA TTTTAGTGTG TGGCCATTCC TTGGAAGTGA ATATAACCAC AAACCTGGAC
    TTCTTCCTAA GTGTGGCTCA AGTTCAACTC TTACATCAGT TAATAGTAGC AAATATGACT
    GGACTGGAAC CATCAAACAA AGCCACAGAG ATCGCTAAGC AAGAACAGAA AAAAATGGAT
    ACATATGATG GAATCGTGGC TGATAGCTCA TCTCGGTACA GTGGTGCCCA GGATAGTGGA
    ATCGGCAGTG ACAGCGTTAA AATCAGAATA GTACAGATAG AGCAGCACAG CGGTGCCAGT
    CAGCACCGCA TCGCCCGTCC CTCACGCCAG TCGTCAATTG TAAAAAATCT AAATTTTATT
    CCTTTTGACA TATTTATTAC TGCAAGTAGA ATCTCACTAA TGACCTATTC CTGTACGGCC
    TTACCCAAAT CGAAATTGCA AGAACAAAAG GATAATGAAA AAACAGGCAA GAGTTCATTA
    AACCTGCCAG AAGTCGATTC AGATGTCGCA AAACCCAGCC AGGCATGTAT TTCCATGGTA
    ACAGCAGAAG ATCTCTTAAG CAGCAACATA TCTTTTCCTT CAGGGAAAAA AATAGGGGTC
    ATCTCTCTCG AAAGTCTGCA TGCATCCACA AGGTCATCTG CTAGACAAGC CCTCGGCATA
    ACCATTGTTC GGCAGCCTGG AAGAAGAGGA ACCGGTGACT TGCGACTAGA ACCTTTCTTG
    TACTTTATTG TATCCCAGCC TTCTTTGCTT CTGAGTTGTC ACCACAGGAA GCAACGAGTG
    GAAATATCCA TTTTTGATGC TGTGCTTAAA GGGGTTGCCT CTGATTACAA ATGTACAGAT
    CCTGGGAAGA CTTTGCCTGA AGCCCTTGAT TATTGCACAG TTTGGCTGCA GACAGTACCT
    GGAGAAATAG ACAGCAAAAG TGGTATTCCA CCTTCACTCT TAACGCTACA AATTAAAGAT
    TTTCTTAACG GACCAGCAGA CATCAACTTG GATATATCAA AGCCTTTGAA AGCAAACCTG
    AGTTTTACCA AGTTGGATCA AATAAACCAT TTTTTGAAGA AGATAAAAAA TGTACACAGC
    AAAGCGTCCT CAGCACCGTC AGACGCTGTG CTGAGTAAGG ATGCGCTTCC AGCCTCCAAA
    TGTTACCGTG GGAAGTTATC CAAGCTTAAA GTTCATGGTG ATGGAGCACA AAAGATTCCA
    TTTCAAGAAA ACATATGGAG AACGATGTCC TGCTTTCAAA AAATTTCTGT TCATACTACT
    CAGATTGTGG TCTCCATGGA AACTGTCCCC CATCCTAATA AACCATGCCT GTTGGCATCT
    TTATCCACCC TCAGTGGAAG CCTTAATGTC AAAGCAGCGC AGAAAGTACC TGGTGTGATT
    CTTGGGTCAT CTTTTCTACT CAGCGTAAAT GATTTTCTTC TTAAAACGTG TCTCAAAGAA
    AGAAGTCGGA TTCTAATAGG ACCGTGTTGT GCTACTGCCA ATTTGGAAGC TAAATGGTGT
    AAACACAGTG GCGATCCAGG CCCAGAACAG TCCATACCAA AAATATCCAT TGATGTAAGA
    GGAGGTCTGC TACAGGTTTT CTGGGGTCAA GAACATTTGA ATTGCTTAAT TCTTCTGCAT
    GAATTACTCA GTGGATACCT TAACATGGAA GGAAATTTTG AAGCGAAAGT TTCTGAAGCA
    GTTCCACAAA TGCCATCTCC TTTGGAAAAA AATCAGATAT TTAAAAGTGA ACAAAGTTCA
    GATGACCTAC GGACAGGTCT ATTTCAGTAT ATACGGGATG CTGAACCTCT GAAAATGCCT
    GGTGTCTATG AAGTCTTGTT TTACAATGAA ACCGAAGATA GCCCAGGGAT GATGCTGTGG
    AGATACCCAG AACCTAGAGT GCTCACCCTT GTACGCATAA CTCCTGTGCC CTTCAACACC
    ACCGAGGATC CAGACATTAG CACAGCAGAC CTTGGTGACA TGCTGCAGAT TCCTTGTAGT
    TTGGAATACT GGGATGAACT CCAGAAGGTT TTTGTTGCAT TTCGAGAATT TAGTCTGTCT
    GAAAGCAAAG TTTGTGAACT GCAATTGCCA GATATCAATC TTGTGAACGA CCAGAAGAAA
    CTGGTATCCT CGGATCTTTG GAGAATTGTC TTGAACAGCA GCCAAAATGG AGCTGATGAC
    CAAAGCTCTG CAAGTGAATC TGGTTCTCAA AGCACTTGTG ACCAGCTTGT AACCCCAACA
    GCTCTGGCTG CCTGTACCAG AGTTGACTCC TGCTTTACCC CGTGGTTTGT CCCATCTCTT
    TGCATTTCCT TCCAGTTTGC TCACCTGGAG TTCCATCTTT GTCATCACCT TGACCAGCTT
    GGCACAGCTC CACCAAAATA CCTACAGCCA TTTATTTCTG ATAAAAATGT GCCATCTGAA
    CTAGAATACA TGGTTATTTC CTTCAAAGAA CCACACATGT ATCTTCGACA GTGGAACAAT
    AGTTCTGTCT GTCAGGAGAT CCAGTTCTCA GCTCAAGCAG ACTGTAAACT TCTAGAGTGC
    AGAAATGTGA CAATGCAAAG CTTGGTGAAA CCCTTCAGCA TCTTTGGACA GACTGCAATT
    TCCAGCAATG CAGTAGAAAA GCTGATTGAT TGCTCCATCA TCATTGACTC AATATTTGTA
    AACTTTGGGC AACATGTAGT TCATTCTCTC AACACTGCAG TACAAGCTTG GCAACAGAAC
    AAATGCCCTG AGGTAGAGGA GTTGGTCTTC AGCCATTTTG TGATCTGTAA TGACACACAG
    GAGACACTGC GGTTTGGCCA GGTGGATACT GATGAAAATA TCCTGCTGGC CAGTCTCTGC
    AGTCACCAGT ACAGCTGGCG CTCTCACAAA TCCCCACAGC TGTTACACAT CTGTATTGAA
    GGTTGGGGCA ACTGGCGTTG GTCAGAGCCC TTCAGTGTGG ACCATGCGGG GACATTTATT
    AGAACTATTC AGTACAAGGG TCGAACTGCT TCACTCATCA TCAAGGCTCA GCAAGTCAGC
    GGCGTGCAAA AACAGATTAT CATCTGTGGA AGACAGATCA TCTGTAGTTA CTTGTCTCAA
    AGCATAGAAT TAAAAGTGGT TCAGCATTAC ATTGGTCAAG ATGGACAAGC TGTGGTTCGA
    GAACACTTTG ACTGCCTCAC AGCCAAACAG AAATTGCCTT CGTACATACT AGAGAACAGC
    GAACTGACTG AGTTGTGTGT GAGGGCCAAA GGAGATGAAG ACTGGTCGAG AGACGTGTGC
    CTGGAATCCA AGGCTCCTGA GTATAGTGTC GTCATTCAGG TGCCATCTTC AAACAGTTCC
    ATTATTTATG TCTGGTGCAC AGTTTTGACT TTAGAACCCA GCTCTCAAGT GCAGCAGCGA
    ATGATTGTGT TCAGCCCTCT TTTTATCATG AGGAGTCATC TTCCAGACCC CATTATCATA
    CATTTGGAGA AAAGGAGTCT GGGATTGAGT GAAACACAAA TTATTCCAGG AAAAGGACAG
    GAAAAACCAC TGCAAAACAT AGAACCTGAC CTTGTACATC ACCTAACATT TCAAGCAAGA
    GAAGAGGATG ACCCTTCACA CTGTGCCGTT CCCATCTCAA CATCCCTCAT TAAGCAGATA
    GCCACTAAGA TACACCCCGG AGGCACAGTT ACTCAGATCC TTGATGAGTT CTACGGGCCA
    GAGAAGTTCC TTCAACCCAC ATGGCCCTAT AATAAAAAGG ACTCTGACAG GAATGAGCAA
    CTGAGCCAGT GGGACAGCCC AATGCGAGTG AAGCTGTCAG CCTGGAAGCC ATATGTGAGG
    ACCTTATTGA TAGAATTGCT ACCCTGGGCC CTGCTTATCA ATCAGTCCAA GTGGGACCTC
    TGGCTGTTTG AGGGAGAGAA GATTGTTCTA CAGGTTCCTG CTGGCAAAAT TATTATCCCT
    CCTAATTTTC AGGAAGCTTT TCAAATTGGA ATATACTGGG CAAGTACAAA CACCGTGCAC
    AAGTCGGTAG CTGTTAAGCT GGTCCATAAC CTGACATCTC CAAAGTGGAA AGACGGAGGT
    AACGGCGAAG TTGTATCTTT GGACGAGGAA GGGTTTGTTC ATGCTGAAAT AAGACTCGGT
    GCTTTCCCAG GACACCAGAA GTTGTGCCAG TTCTGCATTT CCTCCATGGT ACAGCAAGGC
    ATACAGATTA TTCAGATTGA AGATAAGACC ACAATAATCA ATAACACACC ATATCAGATA
    TTTTATAAGC CACAGTTAAC TGTCACCAAA CTCCACTCTG GAAAAGAGTA TTTTCATGTT
    CCAGACAGCG CTACTTTCAG CATTTGCCCG GGTGGCGAGC AGCCTGCCGT GAGATCCAGC
    TCCCTTCCTT GCTGGGACCT AATGCCCGAC CTCGGCCAGT CCGCGCTGGA CGCGTCCCTG
    CTGCAGAAGC AGGTCTTGCT GGCCTTTTCT CCCACCGCCG TTGCCGACAG CGCCCAGTGC
    TGGAGCCTGC CAGCCCTAGT CAGACAGGAG CTTCCCAGAC AGAGCGTGGC AGTGCCCTTG
    GGGAACTACA GGGAAGATGG CTTCTGTACC AGGGCAATAG CACTAACATA TCAAGAACAC
    CTTGGAGTGA CTTATTTAAC CCTCTCAGAA GACCCTAGTC CTCGAATAAT TTTCCACAAC
    AGATGTCCAG TGACAATACT TATAAAGGAA AATACTAAAG ATACTCCGAA GTTTGAGGTT
    TATTGCAGAA AAATTCCTCC TGAGTGCTCA ATTCATCATG AGCTATATCA TCAGATTTCC
    AGTTATCCAG ACTGCAAGAC CAGAGACCTC CTTCCTAGCC TCCTCTTGAG AGTGGAATTG
    CTGGAGGAAA TGACAACCGA GTGGAGTGAC GCCATCGACA TCAACAATCA GGGGACACAG
    GTTGTGTTTC TGACTGGCCT TGGCTATGTG TACGTGGACA CCATCCATCA CTGTGGCACA
    GTCTTCATCA ACGTGGCCCC AGAAGGAAAA GCTGGGCCTG TCGTAACTAA TACCAACAGA
    ACTCTGGAGA AGATCGTGAC ATTTAAAATG TTCATCACTC AGTTGAGCCT GGCGGTGTTT
    GATGACCTCA CCCACCACAG AGCATCATCC GAGCTTTTGA GACTCACACT GGACCACGTT
    TTCCTCCAGA TGGCCCCCGT GGCTGGTCAC CTCCCTGGGG AAGAGATGGC CGCCGCCCCC
    TTTCACGTCT ACTCCGTGGA GGTCTGCTGC GGGGACCTGC AGCTGGACAA CCAGCTGTAC
    AACAAATCCA ATTTCCACTT TGCTGTCTTG GTCTGCCAGG AGGAGAAAAC AGAATCTCCT
    CAGTACTCCA AGATGCAGAG TCTCCTCGTA TCCAGTGAAG ATTTGGAAGA ATACAAGAAA
    AACTGCTTTA TCAAACTCTG CCTCACGTTA ACCGAAGGCA AGGATGTCCT GTTCGATGTG
    AACGAGTTCA GTTTTGAATT GAAACCTGCC CGGTTATATG TGGAAGACAC GTTTGTCTAC
    TACATCAAAA CTCTGTTTGA GACCTACCTG CCCAGCAGCA GCCCGGGCGC TCAGCCCACG
    GCTCTCGCAG GCGGCAGACA GGTGCTGCCC ATGCAGGTGA GGCAGCACGC CCGGGCCTTG
    GTGCATCCCG TGAAGCTGCA GAAGCTGGTG ATACAGCCTG TGAACCTCCT CGTCAGCATC
    CATGCGTCCC TCAAGCTGTA CATCGCCTCT GACCACACTC CGCTCTCCTT CTCAGTGTTT
    GAAAGAGGCC CCATCTTCAC CACGGCCAGG CAGCTCGTCC ACGCCCTGGC CATGCACTAC
    GCCGCAGGGG CCCTCTTCAG AGCAGGCTGG GTGGTTGGAT CCCTGGAAAT CCTTGGCAGC
    CCGGCGAGTC TGGTGCGAAG CATCGGGAAC GGCATCGCTG ACTTCTTCAG GCTTCCGTAC
    GAGGGGCTGA CCCGAGGCCC GGGAGCCTTC GTGAGCGGGG TTTCCAGAGG GACTACATCG
    TTTGTGAAGC ACATTTCCAA AGGTACCCTC ACGTCCATCA CCAACCTGGC CACGAGCCTG
    GCCCGGAACA TGGACCGGCT GTCGCTGGAC GAGGAGCACT ACAACCGGCA GGAAGAGTGG
    CGGCGGCAGC TCCCGGAGAG CCTGGGCGAG GGGCTCCGGC AGGGCCTGTC CCGGCTGGGC
    ATCAGTCTGC TCGGTGCAAT TGCTGGTATA GTTGATCAGC CAATGCAGAA CTTCCAGAAA
    ACATCAGAGG CTCAAGCTTC AGCAGGACAC AAGGCCAAGG GTGTTATCTC CGGCGTGGGG
    AAAGGAATCA TGGGGGTGTT CACGAAGCCC ATCGGAGGGG CTGCTGAGCT GGTGTCACAG
    ACTGGCTATG GTATTTTACA CGGAGCTGGA CTTTCTCAGC TTCCCAAACA GCGCTATCAG
    CCAAGTGATC TGCACGCTGA CCAGGCTCCA AACAGCCACG TCAAATACGT CTGGAAGATG
    CTCCAGTCTC TGGGCAGACC GGAAGTACAC ATGGCCCTGG AGGTTGTTCT GGTGAGGGGC
    TCGGGCCAGG AGCACGAGGG CTGCCTGCTG CTGACGTCAG ACGTGCTCTT CGTGGTGAGC
    GTCAGCGAGG ACACCCAGCA GCAGGCCTTC CCAGTCACGG AGATCGACTG CGCACAGGAC
    CGCGAGCAGA GCAGCCTGCT CATCGTGCAG CTCAAGCAGC CCAGAGTGGC CTGTGACGCA
    GAGGTGGATG GAGTTCGAGA GAGACTGTCA GAGCAACAGT ATAACAGACT TGTGGACTAC
    ATCACAAAGA CCTCTTGTCA CCTGGGCCCC GGCTGCTCCT CCGTGCAGGC GTCATGCCCT
    GTGGTGGCTG CGGAACCGCT CCCCTCCACT GTGAAAACAT ACCATTATAT GGTTGATCCA
    CATTTTGCCC AAGTTTTCAT TAGTAAATTT ACCATGGTGA AAAATAAAGC CCTGAGGAAA
    GGGTTCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006055297.1
    CDS1..8292
    Translation

    Target ORF information:

    RefSeq Version XM_006055235.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting-associated protein 13B-like (LOC102406246), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006055235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGATCGTTT CCAAACGAAC GGCCCAGAGC CGACAGTGCG TGCTGCTGGT GGAGCAGGCA 
    GTGGAGAGTC CAAGAGCTCT ACCCACAGGA GGCGTCTGCA GGAATGGAGA TTCTGTACAA
    GCTGTTGATG ATTCACCATT CTCAGATTCT GTTACCTTGG AACAGACTAC AAGTAACATT
    GGTGGATCCA GTGGTCGTGT TAGTCTGTGG ATGCAGTGGG TGCTCCCCAA AATTACTGTA
    AAGCTCTTTG CTCCAGATCC TGACAGTAAA GGCATAGAAC TTTGTATGGT CAGTGAACTA
    GAAGATCTCA GTGCTTCAAT AGATGTCCAG GATGTGTACA CTAAAGTGAA ATGTAAAATA
    GAAAGTTTCA ATATTGATCA CTATAGAAGC AACCTTGGGG AAGAGTGTTG GTCTCTGGGG
    CAATGTGGAG GTGTCTTCCT TTCCTGTACT GACAAGCTGA ACAGACGCAC CTTGTTGGTT
    CGACCCATCA GCAAGCAGGA CCCTTTCAGT AATTGCTCTG GCTTCTTTCC TTCTACAACT
    GCAAAGCTTC TAGATGGCTC TCATCAGCAG CATGGATTTC TGTCTTTGAC ATACACAAAA
    GCTGTAACAA AAAATGTTCG CCACAAGTTA ACATCAAGAA ATGAGCGAAG AAGTTTTCAT
    AAGTTATCTG AAGGTTTAAC AGATGGCTCC CCTCATTTTC TTCATGAAAT ACTTCTTTCA
    GCACAAGCCT TTGATATTGT CCTTTGTTTT CCTTTACTTA ATGCCATTGC AAGTATATTT
    CAAGCAAAAC TACCAAGGAC CCAAAAAGAG AAAAGAAAAT CTCCTGGTCA GCCCATGAGG
    ACCCATACAC TGACTTCACG CAATTTACCT TTGATTTATA TCAACACAAG CGTAATCAGA
    ATTTTTGTTC CAAAAACAGA AGAAATGCAG CCAAGTATTG AAGTTAATCA GACAGCAAAG
    GAAGATACCA TGGTTCTGAA GATTGGCTCT GTTGCTATGG CTCCCCAGGC TGACAATCCC
    CTTGGCAGAT CCGTCCTCAG GAAAGATATT TACCAAAGAG CCTTGAATTT AGGAATTCTT
    CGAGACCCTG GGTCAGAGAT TGAAGACCGA CAATACCAAA TAGATCTGCA GTCGATCAAT
    ATTGGTACTG CTCAGTGGGA TCAACTGAAA CCAGAAAAGG AAAATGGCAC AGGAGGAGTG
    CTAACAGAGA CTGAAAGAAA TTCGCAGAAT CCAGCCCTTG AGTGGAATAT GGCCAGCAGC
    ATACGGCGGC ATCAAGAAAG AAGAGCAATT TTGACTCCCA TTTTAACAGA TTTTTCTGTA
    CGAATAACTG GAGCACCTGC CATCATTTTC ACCAAAATAA TTTCTCCGGA AAATATGCAC
    ACTGAGGAGA TTTTAGTGTG TGGCCATTCC TTGGAAGTGA ATATAACCAC AAACCTGGAC
    TTCTTCCTAA GTGTGGCTCA AGTTCAACTC TTACATCAGT TAATAGTAGC AAATATGACT
    GGACTGGAAC CATCAAACAA AGCCACAGAG ATCGCTAAGC AAGAACAGAA AAAAATGGAT
    ACATATGATG GAATCGTGGC TGATAGCTCA TCTCGGTACA GTGGTGCCCA GGATAGTGGA
    ATCGGCAGTG ACAGCGTTAA AATCAGAATA GTACAGATAG AGCAGCACAG CGGTGCCAGT
    CAGCACCGCA TCGCCCGTCC CTCACGCCAG TCGTCAATTG TAAAAAATCT AAATTTTATT
    CCTTTTGACA TATTTATTAC TGCAAGTAGA ATCTCACTAA TGACCTATTC CTGTACGGCC
    TTACCCAAAT CGAAATTGCA AGAACAAAAG GATAATGAAA AAACAGGCAA GAGTTCATTA
    AACCTGCCAG AAGTCGATTC AGATGTCGCA AAACCCAGCC AGGCATGTAT TTCCATGGTA
    ACAGCAGAAG ATCTCTTAAG CAGCAACATA TCTTTTCCTT CAGGGAAAAA AATAGGGGTC
    ATCTCTCTCG AAAGTCTGCA TGCATCCACA AGGTCATCTG CTAGACAAGC CCTCGGCATA
    ACCATTGTTC GGCAGCCTGG AAGAAGAGGA ACCGGTGACT TGCGACTAGA ACCTTTCTTG
    TACTTTATTG TATCCCAGCC TTCTTTGCTT CTGAGTTGTC ACCACAGGAA GCAACGAGTG
    GAAATATCCA TTTTTGATGC TGTGCTTAAA GGGGTTGCCT CTGATTACAA ATGTACAGAT
    CCTGGGAAGA CTTTGCCTGA AGCCCTTGAT TATTGCACAG TTTGGCTGCA GACAGTACCT
    GGAGAAATAG ACAGCAAAAG TGGTATTCCA CCTTCACTCT TAACGCTACA AATTAAAGAT
    TTTCTTAACG GACCAGCAGA CATCAACTTG GATATATCAA AGCCTTTGAA AGCAAACCTG
    AGTTTTACCA AGTTGGATCA AATAAACCAT TTTTTGAAGA AGATAAAAAA TGTACACAGC
    AAAGCGTCCT CAGCACCGTC AGACGCTGTG CTGAGTAAGG ATGCGCTTCC AGCCTCCAAA
    TGTTACCGTG GGAAGTTATC CAAGCTTAAA GTTCATGGTG ATGGAGCACA AAAGATTCCA
    TTTCAAGAAA ACATATGGAG AACGATGTCC TGCTTTCAAA AAATTTCTGT TCATACTACT
    CAGATTGTGG TCTCCATGGA AACTGTCCCC CATCCTAATA AACCATGCCT GTTGGCATCT
    TTATCCACCC TCAGTGGAAG CCTTAATGTC AAAGCAGCGC AGAAAGTACC TGGTGTGATT
    CTTGGGTCAT CTTTTCTACT CAGCGTAAAT GATTTTCTTC TTAAAACGTG TCTCAAAGAA
    AGAAGTCGGA TTCTAATAGG ACCGTGTTGT GCTACTGCCA ATTTGGAAGC TAAATGGTGT
    AAACACAGTG GCGATCCAGG CCCAGAACAG TCCATACCAA AAATATCCAT TGATGTAAGA
    GGAGGTCTGC TACAGGTTTT CTGGGGTCAA GAACATTTGA ATTGCTTAAT TCTTCTGCAT
    GAATTACTCA GTGGATACCT TAACATGGAA GGAAATTTTG AAGCGAAAGT TTCTGAAGCA
    GTTCCACAAA TGCCATCTCC TTTGGAAAAA AATCAGATAT TTAAAAGTGA ACAAAGTTCA
    GATGACCTAC GGACAGGTCT ATTTCAGTAT ATACGGGATG CTGAACCTCT GAAAATGCCT
    GGTGTCTATG AAGTCTTGTT TTACAATGAA ACCGAAGATA GCCCAGGGAT GATGCTGTGG
    AGATACCCAG AACCTAGAGT GCTCACCCTT GTACGCATAA CTCCTGTGCC CTTCAACACC
    ACCGAGGATC CAGACATTAG CACAGCAGAC CTTGGTGACA TGCTGCAGAT TCCTTGTAGT
    TTGGAATACT GGGATGAACT CCAGAAGGTT TTTGTTGCAT TTCGAGAATT TAGTCTGTCT
    GAAAGCAAAG TTTGTGAACT GCAATTGCCA GATATCAATC TTGTGAACGA CCAGAAGAAA
    CTGGTATCCT CGGATCTTTG GAGAATTGTC TTGAACAGCA GCCAAAATGG AGCTGATGAC
    CAAAGCTCTG CAAGTGAATC TGGTTCTCAA AGCACTTGTG ACCAGCTTGT AACCCCAACA
    GCTCTGGCTG CCTGTACCAG AGTTGACTCC TGCTTTACCC CGTGGTTTGT CCCATCTCTT
    TGCATTTCCT TCCAGTTTGC TCACCTGGAG TTCCATCTTT GTCATCACCT TGACCAGCTT
    GGCACAGCTC CACCAAAATA CCTACAGCCA TTTATTTCTG ATAAAAATGT GCCATCTGAA
    CTAGAATACA TGGTTATTTC CTTCAAAGAA CCACACATGT ATCTTCGACA GTGGAACAAT
    AGTTCTGTCT GTCAGGAGAT CCAGTTCTCA GCTCAAGCAG ACTGTAAACT TCTAGAGTGC
    AGAAATGTGA CAATGCAAAG CTTGGTGAAA CCCTTCAGCA TCTTTGGACA GACTGCAATT
    TCCAGCAATG CAGTAGAAAA GCTGATTGAT TGCTCCATCA TCATTGACTC AATATTTGTA
    AACTTTGGGC AACATGTAGT TCATTCTCTC AACACTGCAG TACAAGCTTG GCAACAGAAC
    AAATGCCCTG AGGTAGAGGA GTTGGTCTTC AGCCATTTTG TGATCTGTAA TGACACACAG
    GAGACACTGC GGTTTGGCCA GGTGGATACT GATGAAAATA TCCTGCTGGC CAGTCTCTGC
    AGTCACCAGT ACAGCTGGCG CTCTCACAAA TCCCCACAGC TGTTACACAT CTGTATTGAA
    GGTTGGGGCA ACTGGCGTTG GTCAGAGCCC TTCAGTGTGG ACCATGCGGG GACATTTATT
    AGAACTATTC AGTACAAGGG TCGAACTGCT TCACTCATCA TCAAGGCTCA GCAAGTCAGC
    GGCGTGCAAA AACAGATTAT CATCTGTGGA AGACAGATCA TCTGTAGTTA CTTGTCTCAA
    AGCATAGAAT TAAAAGTGGT TCAGCATTAC ATTGGTCAAG ATGGACAAGC TGTGGTTCGA
    GAACACTTTG ACTGCCTCAC AGCCAAACAG AAATTGCCTT CGTACATACT AGAGAACAGC
    GAACTGACTG AGTTGTGTGT GAGGGCCAAA GGAGATGAAG ACTGGTCGAG AGACGTGTGC
    CTGGAATCCA AGGCTCCTGA GTATAGTGTC GTCATTCAGG TGCCATCTTC AAACAGTTCC
    ATTATTTATG TCTGGTGCAC AGTTTTGACT TTAGAACCCA GCTCTCAAGT GCAGCAGCGA
    ATGATTGTGT TCAGCCCTCT TTTTATCATG AGGAGTCATC TTCCAGACCC CATTATCATA
    CATTTGGAGA AAAGGAGTCT GGGATTGAGT GAAACACAAA TTATTCCAGG AAAAGGACAG
    GAAAAACCAC TGCAAAACAT AGAACCTGAC CTTGTACATC ACCTAACATT TCAAGCAAGA
    GAAGAGGATG ACCCTTCACA CTGTGCCGTT CCCATCTCAA CATCCCTCAT TAAGCAGATA
    GCCACTAAGA TACACCCCGG AGGCACAGTT ACTCAGATCC TTGATGAGTT CTACGGGCCA
    GAGAAGTTCC TTCAACCCAC ATGGCCCTAT AATAAAAAGG ACTCTGACAG GAATGAGCAA
    CTGAGCCAGT GGGACAGCCC AATGCGAGTG AAGCTGTCAG CCTGGAAGCC ATATGTGAGG
    ACCTTATTGA TAGAATTGCT ACCCTGGGCC CTGCTTATCA ATCAGTCCAA GTGGGACCTC
    TGGCTGTTTG AGGGAGAGAA GATTGTTCTA CAGGTTCCTG CTGGCAAAAT TATTATCCCT
    CCTAATTTTC AGGAAGCTTT TCAAATTGGA ATATACTGGG CAAGTACAAA CACCGTGCAC
    AAGTCGGTAG CTGTTAAGCT GGTCCATAAC CTGACATCTC CAAAGTGGAA AGACGGAGGT
    AACGGCGAAG TTGTATCTTT GGACGAGGAA GGGTTTGTTC ATGCTGAAAT AAGACTCGGT
    GCTTTCCCAG GACACCAGAA GTTGTGCCAG TTCTGCATTT CCTCCATGGT ACAGCAAGGC
    ATACAGATTA TTCAGATTGA AGATAAGACC ACAATAATCA ATAACACACC ATATCAGATA
    TTTTATAAGC CACAGTTAAC TGTCACCAAA CTCCACTCTG GAAAAGAGTA TTTTCATGTT
    CCAGACAGCG CTACTTTCAG CATTTGCCCG GGTGGCGAGC AGCCTGCCGT GAGATCCAGC
    TCCCTTCCTT GCTGGGACCT AATGCCCGAC CTCGGCCAGT CCGCGCTGGA CGCGTCCCTG
    CTGCAGAAGC AGGTCTTGCT GGCCTTTTCT CCCACCGCCG TTGCCGACAG CGCCCAGTGC
    TGGAGCCTGC CAGCCCTAGT CAGACAGGAG CTTCCCAGAC AGAGCGTGGC AGTGCCCTTG
    GGGAACTACA GGGAAGATGG CTTCTGTACC AGGGCAATAG CACTAACATA TCAAGAACAC
    CTTGGAGTGA CTTATTTAAC CCTCTCAGAA GACCCTAGTC CTCGAATAAT TTTCCACAAC
    AGATGTCCAG TGACAATACT TATAAAGGAA AATACTAAAG ATACTCCGAA GTTTGAGGTT
    TATTGCAGAA AAATTCCTCC TGAGTGCTCA ATTCATCATG AGCTATATCA TCAGATTTCC
    AGTTATCCAG ACTGCAAGAC CAGAGACCTC CTTCCTAGCC TCCTCTTGAG AGTGGAATTG
    CTGGAGGAAA TGACAACCGA GTGGAGTGAC GCCATCGACA TCAACAATCA GGGGACACAG
    GTTGTGTTTC TGACTGGCCT TGGCTATGTG TACGTGGACA CCATCCATCA CTGTGGCACA
    GTCTTCATCA ACGTGGCCCC AGAAGGAAAA GCTGGGCCTG TCGTAACTAA TACCAACAGA
    ACTCTGGAGA AGATCGTGAC ATTTAAAATG TTCATCACTC AGTTGAGCCT GGCGGTGTTT
    GATGACCTCA CCCACCACAG AGCATCATCC GAGCTTTTGA GACTCACACT GGACCACGTT
    TTCCTCCAGA TGGCCCCCGT GGCTGGTCAC CTCCCTGGGG AAGAGATGGC CGCCGCCCCC
    TTTCACGTCT ACTCCGTGGA GGTCTGCTGC GGGGACCTGC AGCTGGACAA CCAGCTGTAC
    AACAAATCCA ATTTCCACTT TGCTGTCTTG GTCTGCCAGG AGGAGAAAAC AGAATCTCCT
    CAGTACTCCA AGATGCAGAG TCTCCTCGTA TCCAGTGAAG ATTTGGAAGA ATACAAGAAA
    AACTGCTTTA TCAAACTCTG CCTCACGTTA ACCGAAGGCA AGGATGTCCT GTTCGATGTG
    AACGAGTTCA GTTTTGAATT GAAACCTGCC CGGTTATATG TGGAAGACAC GTTTGTCTAC
    TACATCAAAA CTCTGTTTGA GACCTACCTG CCCAGCAGCA GCCCGGGCGC TCAGCCCACG
    GCTCTCGCAG GCGGCAGACA GGTGCTGCCC ATGCAGGTGA GGCAGCACGC CCGGGCCTTG
    GTGCATCCCG TGAAGCTGCA GAAGCTGGTG ATACAGCCTG TGAACCTCCT CGTCAGCATC
    CATGCGTCCC TCAAGCTGTA CATCGCCTCT GACCACACTC CGCTCTCCTT CTCAGTGTTT
    GAAAGAGGCC CCATCTTCAC CACGGCCAGG CAGCTCGTCC ACGCCCTGGC CATGCACTAC
    GCCGCAGGGG CCCTCTTCAG AGCAGGCTGG GTGGTTGGAT CCCTGGAAAT CCTTGGCAGC
    CCGGCGAGTC TGGTGCGAAG CATCGGGAAC GGCATCGCTG ACTTCTTCAG GCTTCCGTAC
    GAGGGGCTGA CCCGAGGCCC GGGAGCCTTC GTGAGCGGGG TTTCCAGAGG GACTACATCG
    TTTGTGAAGC ACATTTCCAA AGGTACCCTC ACGTCCATCA CCAACCTGGC CACGAGCCTG
    GCCCGGAACA TGGACCGGCT GTCGCTGGAC GAGGAGCACT ACAACCGGCA GGAAGAGTGG
    CGGCGGCAGC TCCCGGAGAG CCTGGGCGAG GGGCTCCGGC AGGGCCTGTC CCGGCTGGGC
    ATCAGTCTGC TCGGTGCAAT TGCTGGTATA GTTGATCAGC CAATGCAGAA CTTCCAGAAA
    ACATCAGAGG CTCAAGCTTC AGCAGGACAC AAGGCCAAGG GTGTTATCTC CGGCGTGGGG
    AAAGGAATCA TGGGGGTGTT CACGAAGCCC ATCGGAGGGG CTGCTGAGCT GGTGTCACAG
    ACTGGCTATG GTATTTTACA CGGAGCTGGA CTTTCTCAGC TTCCCAAACA GCGCTATCAG
    CCAAGTGATC TGCACGCTGA CCAGGCTCCA AACAGCCACG TCAAATACGT CTGGAAGATG
    CTCCAGTCTC TGGGCAGACC GGAAGTACAC ATGGCCCTGG AGGTTGTTCT GGTGAGGGGC
    TCGGGCCAGG AGCACGAGGG CTGCCTGCTG CTGACGTCAG ACGTGCTCTT CGTGGTGAGC
    GTCAGCGAGG ACACCCAGCA GCAGGCCTTC CCAGTCACGG AGATCGACTG CGCACAGGAC
    CGCGAGCAGA GCAGCCTGCT CATCGTGCAG CTCAAGCAGC CCAGAGTGGC CTGTGACGCA
    GAGGTGGATG GAGTTCGAGA GAGACTGTCA GAGCAACAGT ATAACAGACT TGTGGACTAC
    ATCACAAAGA CCTCTTGTCA CCTGGGCCCC GGCTGCTCCT CCGTGCAGGC GTCATGCCCT
    GTGGTGGCTG CGGAACCGCT CCCCTCCACT GTGAAAACAT ACCATTATAT GGTTGATCCA
    CATTTTGCCC AAGTTTTCAT TAGTAAATTT ACCATGGTGA AAAATAAAGC CCTGAGGAAA
    GGGTTCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu48998
    Clone ID Related Accession (Same CDS sequence) XM_025264764.1 , XM_025264766.1 , XM_025264767.1 , XM_025264765.1
    Accession Version XM_025264764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
    2041
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    2641
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    9781
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    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120549.1
    CDS444..12497
    Translation

    Target ORF information:

    RefSeq Version XM_025264764.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu48998
    Clone ID Related Accession (Same CDS sequence) XM_025264764.1 , XM_025264766.1 , XM_025264767.1 , XM_025264765.1
    Accession Version XM_025264766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
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    721
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2581
    2641
    2701
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    9781
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    10501
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    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120551.1
    CDS380..12433
    Translation

    Target ORF information:

    RefSeq Version XM_025264766.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu48998
    Clone ID Related Accession (Same CDS sequence) XM_025264764.1 , XM_025264766.1 , XM_025264767.1 , XM_025264765.1
    Accession Version XM_025264767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
    1441
    1501
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    1981
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    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120552.1
    CDS57..12110
    Translation

    Target ORF information:

    RefSeq Version XM_025264767.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu48998
    Clone ID Related Accession (Same CDS sequence) XM_025264764.1 , XM_025264766.1 , XM_025264767.1 , XM_025264765.1
    Accession Version XM_025264765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1741
    1801
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    1981
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    11401
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    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120550.1
    CDS175..12228
    Translation

    Target ORF information:

    RefSeq Version XM_025264765.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGTTGTGTT TCTGACTGGC
    CTTGGCTATG TGTACGTGGA CACCATCCAT CACTGTGGCA CAGTCTTCAT CAACGTGGCC
    CCAGAAGGAA AAGCTGGGCC TGTCGTAACT AATACCAACA GAACTCTGGA GAAGATCGTG
    ACATTTAAAA TGTTCATCAC TCAGTTGAGC CTGGCGGTGT TTGATGACCT CACCCACCAC
    AGAGCATCAT CCGAGCTTTT GAGACTCACA CTGGACCACG TTTTCCTCCA GATGGCCCCC
    GTGGCTGGTC ACCTCCCTGG GGAAGAGATG GCCGCCGCCC CCTTTCACGT CTACTCCGTG
    GAGGTCTGCT GCGGGGACCT GCAGCTGGAC AACCAGCTGT ACAACAAATC CAATTTCCAC
    TTTGCTGTCT TGGTCTGCCA GGAGGAGAAA ACAGAATCTC CTCAGTACTC CAAGATGCAG
    AGTCTCCTCG TATCCAGTGA AGATTTGGAA GAATACAAGA AAAACTGCTT TATCAAACTC
    TGCCTCACGT TAACCGAAGG CAAGGATGTC CTGTTCGATG TGAACGAGTT CAGTTTTGAA
    TTGAAACCTG CCCGGTTATA TGTGGAAGAC ACGTTTGTCT ACTACATCAA AACTCTGTTT
    GAGACCTACC TGCCCAGCAG CAGCCCGGGC GCTCAGCCCA CGGCTCTCGC AGGCGGCAGA
    CAGGTGCTGC CCATGCAGGT GAGGCAGCAC GCCCGGGCCT TGGTGCATCC CGTGAAGCTG
    CAGAAGCTGG TGATACAGCC TGTGAACCTC CTCGTCAGCA TCCATGCGTC CCTCAAGCTG
    TACATCGCCT CTGACCACAC TCCGCTCTCC TTCTCAGTGT TTGAAAGAGG CCCCATCTTC
    ACCACGGCCA GGCAGCTCGT CCACGCCCTG GCCATGCACT ACGCCGCAGG GGCCCTCTTC
    AGAGCAGGCT GGGTGGTTGG ATCCCTGGAA ATCCTTGGCA GCCCGGCGAG TCTGGTGCGA
    AGCATCGGGA ACGGCATCGC TGACTTCTTC AGGCTTCCGT ACGAGGGGCT GACCCGAGGC
    CCGGGAGCCT TCGTGAGCGG GGTTTCCAGA GGGACTACAT CGTTTGTGAA GCACATTTCC
    AAAGGTACCC TCACGTCCAT CACCAACCTG GCCACGAGCC TGGCCCGGAA CATGGACCGG
    CTGTCGCTGG ACGAGGAGCA CTACAACCGG CAGGAAGAGT GGCGGCGGCA GCTCCCGGAG
    AGCCTGGGCG AGGGGCTCCG GCAGGGCCTG TCCCGGCTGG GTATCAGTCT GCTCGGTGCA
    ATTGCTGGTA TAGTTGATCA GCCAATGCAG AACTTCCAGA AAACATCAGA GGCTCAAGCT
    TCAGCAGGAC ACAAGGCCAA GGGTGTTATC TCCGGCGTGG GGAAAGGAAT CATGGGGGTG
    TTCACGAAGC CCATCGGAGG GGCTGCTGAG CTGGTGTCAC AGACTGGCTA TGGTATTTTA
    CACGGAGCTG GACTTTCTCA GCTTCCCAAA CAGCGCTATC AGCCAAGTGA TCTGCACGCT
    GACCAGGCTC CAAACAGCCA CGTCAAATAC GTCTGGAAGA TGCTCCAGTC TCTGGGCAGA
    CCGGAAGTAC ACATGGCCCT GGAGGTTGTT CTGGTGAGGG GCTCGGGCCA GGAGCACGAG
    GGCTGCCTGC TGCTGACGTC AGACGTGCTC TTCGTGGTGA GCGTCAGCGA GGACACCCAG
    CAGCAGGCCT TCCCAGTCAC GGAGATCGAC TGCGCACAGG ACCGCGAGCA GAGCAGCCTG
    CTCATCGTGC AGCTCAAGCA GCCCAGAGTG GCCTGTGACG CAGAGGTGGA TGGAGTTCGA
    GAGAGACTGT CAGAGCAACA GTATAACAGA CTTGTGGACT ACATCACAAA GACCTCTTGT
    CACCTGGGCC CCGGCTGCTC CTCCGTGCAG GCGTCATGCC CTGTGGTGGC TGCGGAACCG
    CTCCCCTCCA CTGTGAAAAC ATACCATTAT ATGGTTGATC CACATTTTGC CCAAGTTTTC
    ATTAGTAAAT TTACCATGGT GAAAAATAAA GCCCTGAGGA AAGGGTTCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu48999
    Clone ID Related Accession (Same CDS sequence) XM_025264768.1
    Accession Version XM_025264768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
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    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGAC
    ATCAACTTGG ATATATCAAA GCCTTTGAAA GCAAACCTGA GTTTTACCAA GTTGGATCAA
    ATAAACCATT TTTTGAAGAA GATAAAAAAT GTACACAGCA AAGCGTCCTC AGCACCGTCA
    GACGCTGTGC TGAGTAAGGA TGCGCTTCCA GCCTCCAAAT GTTACCGTGG GAAGTTATCC
    AAGCTTAAAG TTCATGGTGA TGGAGCACAA AAGATTCCAT TTCAAGAAAA CATATGGAGA
    ACGATGTCCT GCTTTCAAAA AATTTCTGTT CATACTACTC AGATTGTGGT CTCCATGGAA
    ACTGTCCCCC ATCCTAATAA ACCATGCCTG TTGGCATCTT TATCCACCCT CAGTGGAAGC
    CTTAATGTCA AAGCAGCGCA GAAAGTACCT GGTGTGATTC TTGGGTCATC TTTTCTACTC
    AGCGTAAATG ATTTTCTTCT TAAAACGTGT CTCAAAGAAA GAAGTCGGAT TCTAATAGGA
    CCGTGTTGTG CTACTGCCAA TTTGGAAGCT AAATGGTGTA AACACAGTGG CGATCCAGGC
    CCAGAACAGT CCATACCAAA AATATCCATT GATGTAAGAG GAGGTCTGCT ACAGGTTTTC
    TGGGGTCAAG AACATTTGAA TTGCTTAATT CTTCTACATG AATTACTCAG TGGATACCTT
    AACATGGAAG GAAATTTTGA AGCGAAAGTT TCTGAAGCAG TTCCACAAAT GCCATCTCCT
    TTGGAAAAAA ATCAGATATT TAAAAGTGAA CAAAGTTCAG ATGACCTACG GACAGGTCTA
    TTTCAGTATA TACGGGATGC TGAACCTCTG AAAATGCCTG GTGTCTATGA AGTCTTGTTT
    TACAATGAAA CCGAAGATAG CCCAGGGATG ATGCTGTGGA GATACCCAGA ACCTAGAGTG
    CTCACCCTTG TACGCATAAC TCCTGTGCCC TTCAACACCA CCGAGGATCC AGACATTAGC
    ACAGCAGACC TTGGTGACAT GCTGCAGATT CCTTGTAGTT TGGAATACTG GGATGAACTC
    CAGAAGGTTT TTGTTGCATT TCGAGAATTT AGTCTGTCTG AAAGCAAAGT TTGTGAACTG
    CAATTGCCAG ATATCAATCT TGTGAACGAC CAGAAGAAAC TGGTATCCTC GGATCTTTGG
    AGAATTGTCT TGAACAGCAG CCAAAATGGA GCTGATGACC AAAGCTCTGC AAGTGAATCT
    GGTTCTCAAA GCACTTGTGA CCAGCTTGTA ACCCCAACAG CTCTGGCTGC CTGTACCAGA
    GTTGACTCCT GCTTTACCCC GTGGTTTGTC CCATCTCTTT GCATTTCCTT CCAGTTTGCT
    CACCTGGAGT TCCATCTTTG TCATCACCTT GACCAGCTTG GCACAGCTCC ACCAAAATAC
    CTACAGCCAT TTATTTCTGA TAAAAATGTG CCATCTGAAC TAGAATACAT GGTTATTTCC
    TTCAAAGAAC CACACATGTA TCTTCGACAG TGGAACAATA GTTCTGTCTG TCAGGAGATC
    CAGTTCTCAG CTCAAGCAGA CTGTAAACTT CTAGAGTGCA GAAATGTGAC AATGCAAAGC
    TTGGTGAAAC CCTTCAGCAT CTTTGGACAG ACTGCAATTT CCAGCAATGC AGTAGAAAAG
    CTGATTGATT GCTCCATCAT CATTGACTCA ATATTTGTAA ACTTTGGGCA ACATGTAGTT
    CATTCTCTCA ACACTGCAGT ACAAGCTTGG CAACAGAACA AATGCCCTGA GGTAGAGGAG
    TTGGTCTTCA GCCATTTTGT GATCTGTAAT GACACACAGG AGACACTGCG GTTTGGCCAG
    GTGGATACTG ATGAAAATAT CCTGCTGGCC AGTCTCTGCA GTCACCAGTA CAGCTGGCGC
    TCTCACAAAT CCCCACAGCT GTTACACATC TGTATTGAAG GTTGGGGCAA CTGGCGTTGG
    TCAGAGCCCT TCAGTGTGGA CCATGCGGGG ACATTTATTA GAACTATTCA GTACAAGGGT
    CGAACTGCTT CACTCATCAT CAAGGCTCAG CAAGTCAGCG GCGTGCAAAA ACAGATTATC
    ATCTGTGGAA GACAGATCAT CTGTAGTTAC TTGTCTCAAA GCATAGAATT AAAAGTGGTT
    CAGCATTACA TTGGTCAAGA TGGACAAGCT GTGGTTCGAG AACACTTTGA CTGCCTCACA
    GCCAAACAGA AATTGCCTTC GTACATACTA GAGAACAGCG AACTGACTGA GTTGTGTGTG
    AGGGCCAAAG GAGATGAAGA CTGGTCAAGA GACGTGTGCC TGGAATCCAA GGCTCCTGAG
    TATAGTGTCG TCATTCAGGT GCCATCTTCA AACAGTTCCA TTATTTATGT CTGGTGCACA
    GTTTTGACTT TAGAACCCAG CTCTCAAGTG CAGCAGCGAA TGATTGTGTT CAGCCCTCTT
    TTTATCATGA GGAGTCATCT TCCAGACCCC ATTATCATAC ATTTGGAGAA AAGGAGTCTG
    GGATTGAGTG AAACACAAAT TATTCCAGGA AAAGGACAGG AAAAACCACT GCAAAACATA
    GAACCTGACC TTGTACATCA CCTAACATTT CAAGCAAGAG AAGAGGATGA CCCTTCACAC
    TGTGCCGTTC CCATCTCAAC ATCCCTCATT AAGCAGATAG CCACTAAGAT ACACCCCGGA
    GGCACAGTTA CTCAGATCCT TGATGAGTTC TACGGGCCAG AGAAGTTCCT TCAACCCACA
    TGGCCCTATA ATAAAAAGGA CTCTGACAGG AATGAGCAAC TGAGCCAGTG GGACAGCCCA
    ATGCGAGTGA AGCTGTCAGC CTGGAAGCCA TATGTGAGGA CCTTATTGAT AGAATTGCTA
    CCCTGGGCCC TGCTTATCAA TCAGTCCAAG TGGGACCTCT GGCTGTTTGA GGGAGAGAAG
    ATTGTTCTAC AGGTTCCTGC TGGCAAAATT ATTATCCCTC CTAATTTTCA GGAAGCTTTT
    CAAATTGGAA TATACTGGGC AAGTACAAAC ACCGTGCACA AGTCAGTAGC TGTTAAGCTG
    GTCCATAACC TGACATCTCC AAAGTGGAAA GACGGAGGTA ACGGCGAAGT TGTATCTTTG
    GACGAGGAAG GGTTTGTTCA TGCTGAAATA AGACTCGGTG CTTTCCCAGG ACACCAGAAG
    TTGTGCCAGT TCTGCATTTC CTCCATGGTA CAGCAAGGCA TACAGATTAT TCAGATTGAA
    GATAAGACCA CAATAATCAA TAACACACCA TATCAGATAT TTTATAAGCC ACAGTTAACT
    GTCACCAAAC TCCACTCTGG AAAAGAGTAT TTTCATGTTC CAGACAGCGC TACTTTCAGC
    ATTTGCCCGG GTGGCGAGCA GCCTGCCGTG AGATCCAGCT CCCTTCCTTG CTGGGACCTA
    ATGCCCGACC TCGGCCAGTC CGCGCTGGAC GCGTCCCTGC TGCAGAAGCA GGTCTTGCTG
    GCCTTTTCTC CCACCGCCGT TGCCGACAGC GCCCAGTGCT GGAGCCTGCC AGCCCTAGTC
    AGACAGGAGC TTCCCAGACA GAGCGTGGCA GTGCCCTTGG GGAACTACAG GGAAGATGGC
    TTCTGTACCA GGGCAATAGC ACTAACATAT CAAGAACACC TTGGAGTGAC TTATTTAACC
    CTCTCAGAAG ACCCTAGTCC TCGAATAATT TTCCACAACA GATGTCCAGT GACAATACTT
    ATAAAGGAAA ATACTAAAGA TACTCCGAAG TTTGAGGTTT ATTGCAGAAA AATTCCTCCT
    GAGTGCTCAA TTCATCATGA GCTATATCAT CAGATTTCCA GTTATCCAGA CTGCAAGACC
    AGAGACCTCC TTCCTAGCCT CCTCTTGAGA GTGGAATTGC TGGAGGAAAT GACAACCGAG
    TGGAGTGACG CCATCGACAT CAACAATCAG GGGACACAGG TTGTGTTTCT GACTGGCCTT
    GGCTATGTGT ACGTGGACAC CATCCATCAC TGTGGCACAG TCTTCATCAA CGTGGCCCCA
    GAAGGAAAAG CTGGGCCTGT CGTAACTAAT ACCAACAGAA CTCTGGAGAA GATCGTGACA
    TTTAAAATGT TCATCACTCA GTTGAGCCTG GCGGTGTTTG ATGACCTCAC CCACCACAGA
    GCATCATCCG AGCTTTTGAG ACTCACACTG GACCACGTTT TCCTCCAGAT GGCCCCCGTG
    GCTGGTCACC TCCCTGGGGA AGAGATGGCC GCCGCCCCCT TTCACGTCTA CTCCGTGGAG
    GTCTGCTGCG GGGACCTGCA GCTGGACAAC CAGCTGTACA ACAAATCCAA TTTCCACTTT
    GCTGTCTTGG TCTGCCAGGA GGAGAAAACA GAATCTCCTC AGTACTCCAA GATGCAGAGT
    CTCCTCGTAT CCAGTGAAGA TTTGGAAGAA TACAAGAAAA ACTGCTTTAT CAAACTCTGC
    CTCACGTTAA CCGAAGGCAA GGATGTCCTG TTCGATGTGA ACGAGTTCAG TTTTGAATTG
    AAACCTGCCC GGTTATATGT GGAAGACACG TTTGTCTACT ACATCAAAAC TCTGTTTGAG
    ACCTACCTGC CCAGCAGCAG CCCGGGCGCT CAGCCCACGG CTCTCGCAGG CGGCAGACAG
    GTGCTGCCCA TGCAGGTGAG GCAGCACGCC CGGGCCTTGG TGCATCCCGT GAAGCTGCAG
    AAGCTGGTGA TACAGCCTGT GAACCTCCTC GTCAGCATCC ATGCGTCCCT CAAGCTGTAC
    ATCGCCTCTG ACCACACTCC GCTCTCCTTC TCAGTGTTTG AAAGAGGCCC CATCTTCACC
    ACGGCCAGGC AGCTCGTCCA CGCCCTGGCC ATGCACTACG CCGCAGGGGC CCTCTTCAGA
    GCAGGCTGGG TGGTTGGATC CCTGGAAATC CTTGGCAGCC CGGCGAGTCT GGTGCGAAGC
    ATCGGGAACG GCATCGCTGA CTTCTTCAGG CTTCCGTACG AGGGGCTGAC CCGAGGCCCG
    GGAGCCTTCG TGAGCGGGGT TTCCAGAGGG ACTACATCGT TTGTGAAGCA CATTTCCAAA
    GGTACCCTCA CGTCCATCAC CAACCTGGCC ACGAGCCTGG CCCGGAACAT GGACCGGCTG
    TCGCTGGACG AGGAGCACTA CAACCGGCAG GAAGAGTGGC GGCGGCAGCT CCCGGAGAGC
    CTGGGCGAGG GGCTCCGGCA GGGCCTGTCC CGGCTGGGTA TCAGTCTGCT CGGTGCAATT
    GCTGGTATAG TTGATCAGCC AATGCAGAAC TTCCAGAAAA CATCAGAGGC TCAAGCTTCA
    GCAGGACACA AGGCCAAGGG TGTTATCTCC GGCGTGGGGA AAGGAATCAT GGGGGTGTTC
    ACGAAGCCCA TCGGAGGGGC TGCTGAGCTG GTGTCACAGA CTGGCTATGG TATTTTACAC
    GGAGCTGGAC TTTCTCAGCT TCCCAAACAG CGCTATCAGC CAAGTGATCT GCACGCTGAC
    CAGGCTCCAA ACAGCCACGT CAAATACGTC TGGAAGATGC TCCAGTCTCT GGGCAGACCG
    GAAGTACACA TGGCCCTGGA GGTTGTTCTG GTGAGGGGCT CGGGCCAGGA GCACGAGGGC
    TGCCTGCTGC TGACGTCAGA CGTGCTCTTC GTGGTGAGCG TCAGCGAGGA CACCCAGCAG
    CAGGCCTTCC CAGTCACGGA GATCGACTGC GCACAGGACC GCGAGCAGAG CAGCCTGCTC
    ATCGTGCAGC TCAAGCAGCC CAGAGTGGCC TGTGACGCAG AGGTGGATGG AGTTCGAGAG
    AGACTGTCAG AGCAACAGTA TAACAGACTT GTGGACTACA TCACAAAGAC CTCTTGTCAC
    CTGGGCCCCG GCTGCTCCTC CGTGCAGGCG TCATGCCCTG TGGTGGCTGC GGAACCGCTC
    CCCTCCACTG TGAAAACATA CCATTATATG GTTGATCCAC ATTTTGCCCA AGTTTTCATT
    AGTAAATTTA CCATGGTGAA AAATAAAGCC CTGAGGAAAG GGTTCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120553.1
    CDS1..12051
    Translation

    Target ORF information:

    RefSeq Version XM_025264768.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGAC
    ATCAACTTGG ATATATCAAA GCCTTTGAAA GCAAACCTGA GTTTTACCAA GTTGGATCAA
    ATAAACCATT TTTTGAAGAA GATAAAAAAT GTACACAGCA AAGCGTCCTC AGCACCGTCA
    GACGCTGTGC TGAGTAAGGA TGCGCTTCCA GCCTCCAAAT GTTACCGTGG GAAGTTATCC
    AAGCTTAAAG TTCATGGTGA TGGAGCACAA AAGATTCCAT TTCAAGAAAA CATATGGAGA
    ACGATGTCCT GCTTTCAAAA AATTTCTGTT CATACTACTC AGATTGTGGT CTCCATGGAA
    ACTGTCCCCC ATCCTAATAA ACCATGCCTG TTGGCATCTT TATCCACCCT CAGTGGAAGC
    CTTAATGTCA AAGCAGCGCA GAAAGTACCT GGTGTGATTC TTGGGTCATC TTTTCTACTC
    AGCGTAAATG ATTTTCTTCT TAAAACGTGT CTCAAAGAAA GAAGTCGGAT TCTAATAGGA
    CCGTGTTGTG CTACTGCCAA TTTGGAAGCT AAATGGTGTA AACACAGTGG CGATCCAGGC
    CCAGAACAGT CCATACCAAA AATATCCATT GATGTAAGAG GAGGTCTGCT ACAGGTTTTC
    TGGGGTCAAG AACATTTGAA TTGCTTAATT CTTCTACATG AATTACTCAG TGGATACCTT
    AACATGGAAG GAAATTTTGA AGCGAAAGTT TCTGAAGCAG TTCCACAAAT GCCATCTCCT
    TTGGAAAAAA ATCAGATATT TAAAAGTGAA CAAAGTTCAG ATGACCTACG GACAGGTCTA
    TTTCAGTATA TACGGGATGC TGAACCTCTG AAAATGCCTG GTGTCTATGA AGTCTTGTTT
    TACAATGAAA CCGAAGATAG CCCAGGGATG ATGCTGTGGA GATACCCAGA ACCTAGAGTG
    CTCACCCTTG TACGCATAAC TCCTGTGCCC TTCAACACCA CCGAGGATCC AGACATTAGC
    ACAGCAGACC TTGGTGACAT GCTGCAGATT CCTTGTAGTT TGGAATACTG GGATGAACTC
    CAGAAGGTTT TTGTTGCATT TCGAGAATTT AGTCTGTCTG AAAGCAAAGT TTGTGAACTG
    CAATTGCCAG ATATCAATCT TGTGAACGAC CAGAAGAAAC TGGTATCCTC GGATCTTTGG
    AGAATTGTCT TGAACAGCAG CCAAAATGGA GCTGATGACC AAAGCTCTGC AAGTGAATCT
    GGTTCTCAAA GCACTTGTGA CCAGCTTGTA ACCCCAACAG CTCTGGCTGC CTGTACCAGA
    GTTGACTCCT GCTTTACCCC GTGGTTTGTC CCATCTCTTT GCATTTCCTT CCAGTTTGCT
    CACCTGGAGT TCCATCTTTG TCATCACCTT GACCAGCTTG GCACAGCTCC ACCAAAATAC
    CTACAGCCAT TTATTTCTGA TAAAAATGTG CCATCTGAAC TAGAATACAT GGTTATTTCC
    TTCAAAGAAC CACACATGTA TCTTCGACAG TGGAACAATA GTTCTGTCTG TCAGGAGATC
    CAGTTCTCAG CTCAAGCAGA CTGTAAACTT CTAGAGTGCA GAAATGTGAC AATGCAAAGC
    TTGGTGAAAC CCTTCAGCAT CTTTGGACAG ACTGCAATTT CCAGCAATGC AGTAGAAAAG
    CTGATTGATT GCTCCATCAT CATTGACTCA ATATTTGTAA ACTTTGGGCA ACATGTAGTT
    CATTCTCTCA ACACTGCAGT ACAAGCTTGG CAACAGAACA AATGCCCTGA GGTAGAGGAG
    TTGGTCTTCA GCCATTTTGT GATCTGTAAT GACACACAGG AGACACTGCG GTTTGGCCAG
    GTGGATACTG ATGAAAATAT CCTGCTGGCC AGTCTCTGCA GTCACCAGTA CAGCTGGCGC
    TCTCACAAAT CCCCACAGCT GTTACACATC TGTATTGAAG GTTGGGGCAA CTGGCGTTGG
    TCAGAGCCCT TCAGTGTGGA CCATGCGGGG ACATTTATTA GAACTATTCA GTACAAGGGT
    CGAACTGCTT CACTCATCAT CAAGGCTCAG CAAGTCAGCG GCGTGCAAAA ACAGATTATC
    ATCTGTGGAA GACAGATCAT CTGTAGTTAC TTGTCTCAAA GCATAGAATT AAAAGTGGTT
    CAGCATTACA TTGGTCAAGA TGGACAAGCT GTGGTTCGAG AACACTTTGA CTGCCTCACA
    GCCAAACAGA AATTGCCTTC GTACATACTA GAGAACAGCG AACTGACTGA GTTGTGTGTG
    AGGGCCAAAG GAGATGAAGA CTGGTCAAGA GACGTGTGCC TGGAATCCAA GGCTCCTGAG
    TATAGTGTCG TCATTCAGGT GCCATCTTCA AACAGTTCCA TTATTTATGT CTGGTGCACA
    GTTTTGACTT TAGAACCCAG CTCTCAAGTG CAGCAGCGAA TGATTGTGTT CAGCCCTCTT
    TTTATCATGA GGAGTCATCT TCCAGACCCC ATTATCATAC ATTTGGAGAA AAGGAGTCTG
    GGATTGAGTG AAACACAAAT TATTCCAGGA AAAGGACAGG AAAAACCACT GCAAAACATA
    GAACCTGACC TTGTACATCA CCTAACATTT CAAGCAAGAG AAGAGGATGA CCCTTCACAC
    TGTGCCGTTC CCATCTCAAC ATCCCTCATT AAGCAGATAG CCACTAAGAT ACACCCCGGA
    GGCACAGTTA CTCAGATCCT TGATGAGTTC TACGGGCCAG AGAAGTTCCT TCAACCCACA
    TGGCCCTATA ATAAAAAGGA CTCTGACAGG AATGAGCAAC TGAGCCAGTG GGACAGCCCA
    ATGCGAGTGA AGCTGTCAGC CTGGAAGCCA TATGTGAGGA CCTTATTGAT AGAATTGCTA
    CCCTGGGCCC TGCTTATCAA TCAGTCCAAG TGGGACCTCT GGCTGTTTGA GGGAGAGAAG
    ATTGTTCTAC AGGTTCCTGC TGGCAAAATT ATTATCCCTC CTAATTTTCA GGAAGCTTTT
    CAAATTGGAA TATACTGGGC AAGTACAAAC ACCGTGCACA AGTCAGTAGC TGTTAAGCTG
    GTCCATAACC TGACATCTCC AAAGTGGAAA GACGGAGGTA ACGGCGAAGT TGTATCTTTG
    GACGAGGAAG GGTTTGTTCA TGCTGAAATA AGACTCGGTG CTTTCCCAGG ACACCAGAAG
    TTGTGCCAGT TCTGCATTTC CTCCATGGTA CAGCAAGGCA TACAGATTAT TCAGATTGAA
    GATAAGACCA CAATAATCAA TAACACACCA TATCAGATAT TTTATAAGCC ACAGTTAACT
    GTCACCAAAC TCCACTCTGG AAAAGAGTAT TTTCATGTTC CAGACAGCGC TACTTTCAGC
    ATTTGCCCGG GTGGCGAGCA GCCTGCCGTG AGATCCAGCT CCCTTCCTTG CTGGGACCTA
    ATGCCCGACC TCGGCCAGTC CGCGCTGGAC GCGTCCCTGC TGCAGAAGCA GGTCTTGCTG
    GCCTTTTCTC CCACCGCCGT TGCCGACAGC GCCCAGTGCT GGAGCCTGCC AGCCCTAGTC
    AGACAGGAGC TTCCCAGACA GAGCGTGGCA GTGCCCTTGG GGAACTACAG GGAAGATGGC
    TTCTGTACCA GGGCAATAGC ACTAACATAT CAAGAACACC TTGGAGTGAC TTATTTAACC
    CTCTCAGAAG ACCCTAGTCC TCGAATAATT TTCCACAACA GATGTCCAGT GACAATACTT
    ATAAAGGAAA ATACTAAAGA TACTCCGAAG TTTGAGGTTT ATTGCAGAAA AATTCCTCCT
    GAGTGCTCAA TTCATCATGA GCTATATCAT CAGATTTCCA GTTATCCAGA CTGCAAGACC
    AGAGACCTCC TTCCTAGCCT CCTCTTGAGA GTGGAATTGC TGGAGGAAAT GACAACCGAG
    TGGAGTGACG CCATCGACAT CAACAATCAG GGGACACAGG TTGTGTTTCT GACTGGCCTT
    GGCTATGTGT ACGTGGACAC CATCCATCAC TGTGGCACAG TCTTCATCAA CGTGGCCCCA
    GAAGGAAAAG CTGGGCCTGT CGTAACTAAT ACCAACAGAA CTCTGGAGAA GATCGTGACA
    TTTAAAATGT TCATCACTCA GTTGAGCCTG GCGGTGTTTG ATGACCTCAC CCACCACAGA
    GCATCATCCG AGCTTTTGAG ACTCACACTG GACCACGTTT TCCTCCAGAT GGCCCCCGTG
    GCTGGTCACC TCCCTGGGGA AGAGATGGCC GCCGCCCCCT TTCACGTCTA CTCCGTGGAG
    GTCTGCTGCG GGGACCTGCA GCTGGACAAC CAGCTGTACA ACAAATCCAA TTTCCACTTT
    GCTGTCTTGG TCTGCCAGGA GGAGAAAACA GAATCTCCTC AGTACTCCAA GATGCAGAGT
    CTCCTCGTAT CCAGTGAAGA TTTGGAAGAA TACAAGAAAA ACTGCTTTAT CAAACTCTGC
    CTCACGTTAA CCGAAGGCAA GGATGTCCTG TTCGATGTGA ACGAGTTCAG TTTTGAATTG
    AAACCTGCCC GGTTATATGT GGAAGACACG TTTGTCTACT ACATCAAAAC TCTGTTTGAG
    ACCTACCTGC CCAGCAGCAG CCCGGGCGCT CAGCCCACGG CTCTCGCAGG CGGCAGACAG
    GTGCTGCCCA TGCAGGTGAG GCAGCACGCC CGGGCCTTGG TGCATCCCGT GAAGCTGCAG
    AAGCTGGTGA TACAGCCTGT GAACCTCCTC GTCAGCATCC ATGCGTCCCT CAAGCTGTAC
    ATCGCCTCTG ACCACACTCC GCTCTCCTTC TCAGTGTTTG AAAGAGGCCC CATCTTCACC
    ACGGCCAGGC AGCTCGTCCA CGCCCTGGCC ATGCACTACG CCGCAGGGGC CCTCTTCAGA
    GCAGGCTGGG TGGTTGGATC CCTGGAAATC CTTGGCAGCC CGGCGAGTCT GGTGCGAAGC
    ATCGGGAACG GCATCGCTGA CTTCTTCAGG CTTCCGTACG AGGGGCTGAC CCGAGGCCCG
    GGAGCCTTCG TGAGCGGGGT TTCCAGAGGG ACTACATCGT TTGTGAAGCA CATTTCCAAA
    GGTACCCTCA CGTCCATCAC CAACCTGGCC ACGAGCCTGG CCCGGAACAT GGACCGGCTG
    TCGCTGGACG AGGAGCACTA CAACCGGCAG GAAGAGTGGC GGCGGCAGCT CCCGGAGAGC
    CTGGGCGAGG GGCTCCGGCA GGGCCTGTCC CGGCTGGGTA TCAGTCTGCT CGGTGCAATT
    GCTGGTATAG TTGATCAGCC AATGCAGAAC TTCCAGAAAA CATCAGAGGC TCAAGCTTCA
    GCAGGACACA AGGCCAAGGG TGTTATCTCC GGCGTGGGGA AAGGAATCAT GGGGGTGTTC
    ACGAAGCCCA TCGGAGGGGC TGCTGAGCTG GTGTCACAGA CTGGCTATGG TATTTTACAC
    GGAGCTGGAC TTTCTCAGCT TCCCAAACAG CGCTATCAGC CAAGTGATCT GCACGCTGAC
    CAGGCTCCAA ACAGCCACGT CAAATACGTC TGGAAGATGC TCCAGTCTCT GGGCAGACCG
    GAAGTACACA TGGCCCTGGA GGTTGTTCTG GTGAGGGGCT CGGGCCAGGA GCACGAGGGC
    TGCCTGCTGC TGACGTCAGA CGTGCTCTTC GTGGTGAGCG TCAGCGAGGA CACCCAGCAG
    CAGGCCTTCC CAGTCACGGA GATCGACTGC GCACAGGACC GCGAGCAGAG CAGCCTGCTC
    ATCGTGCAGC TCAAGCAGCC CAGAGTGGCC TGTGACGCAG AGGTGGATGG AGTTCGAGAG
    AGACTGTCAG AGCAACAGTA TAACAGACTT GTGGACTACA TCACAAAGAC CTCTTGTCAC
    CTGGGCCCCG GCTGCTCCTC CGTGCAGGCG TCATGCCCTG TGGTGGCTGC GGAACCGCTC
    CCCTCCACTG TGAAAACATA CCATTATATG GTTGATCCAC ATTTTGCCCA AGTTTTCATT
    AGTAAATTTA CCATGGTGAA AAATAAAGCC CTGAGGAAAG GGTTCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49000
    Clone ID Related Accession (Same CDS sequence) XM_025264769.1
    Accession Version XM_025264769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAGCCAGG GGAAGGTTGG CAGTCAGGAC ATTTTGAAGG AGTATTTCTA
    CAGTGCAAAG AAAAACCTGT GACAACTGCA AAGCTTCTAG ATGGCTCTCA TCAGCAGCAT
    GGATTTCTGT CTTTGACATA CACAAAAGCT GTAACAAAAA ATGTTCGCCA CAAGTTAACA
    TCAAGAAATG AGCGAAGAAG TTTTCATAAG TTATCTGAAG GTTTAACAGA TGGCTCCCCT
    CATTTTCTTC ATGAAATACT TCTTTCAGCA CAAGCCTTTG ATATTGTCCT TTGTTTTCCT
    TTACTTAATG CCATTGCAAG TATATTTCAA GCAAAACTAC CAAGGACCCA AAAAGAGAAA
    AGAAAATCTC CTGGTCAGCC CATGAGGACC CATACACTGA CTTCACGCAA TTTACCTTTG
    ATTTATATCA ACACAAGCGT AATCAGAATT TTTGTTCCAA AAACAGAAGA AATGCAGCCA
    AGTATTGAAG TTAATCAGAC AGCAAAGGAA GATACCATGG TTCTGAAGAT TGGCTCTGTT
    GCTATGGCTC CCCAGGCTGA CAATCCCCTT GGCAGATCCG TCCTCAGGAA AGATATTTAC
    CAAAGAGCCT TGAATTTAGG AATTCTTCGA GACCCTGGGT CAGAGATTGA AGACCGACAA
    TACCAAATAG ATCTGCAGTC GATCAATATT GGTACTGCTC AGTGGGATCA ACTGAAACCA
    GAAAAGGAAA ATGGCACAGG AGGAGTGCTA ACAGAGACTG AAAGAAATTC GCAGAATCCA
    GCCCTTGAGT GGAATATGGC CAGCAGCATA CGGCGGCATC AAGAAAGAAG AGCAATTTTG
    ACTCCCATTT TAACAGATTT TTCTGTACGA ATAACTGGAG CACCTGCCAT CATTTTCACC
    AAAATAATTT CTCCGGAAAA TATGCACACT GAGGAGATTT TAGTGTGTGG CCATTCCTTG
    GAAGTGAATA TAACCACAAA CCTGGACTTC TTCCTAAGTG TGGCTCAAGT TCAACTCTTA
    CATCAGTTAA TAGTAGCAAA TATGACTGGA CTGGAACCAT CAAACAAAGC CACAGAGATC
    GCTAAGCAAG AACAGAAAAA AATGGATACA TATGATGGAA TCGTGGCTGA TAGCTCATCT
    CGGTACAGTG GTGCCCAGGA TAGTGGAATC GGCAGTGACA GCGTTAAAAT CAGAATAGTA
    CAGATAGAGC AGCACAGCGG TGCCAGTCAG CACCGCATCG CCCGTCCCTC ACGCCAGTCG
    TCAATTGTAA AAAATCTAAA TTTTATTCCT TTTGACATAT TTATTACTGC AAGTAGAATC
    TCACTAATGA CCTATTCCTG TACGGCCTTA CCCAAATCGA AATTGCAAGA ACAAAAGGAT
    AATGAAAAAA CAGGCAAGAG TTCATTAAAC CTGCCAGAAG TCGATTCAGA TGTCGCAAAA
    CCCAGCCAGG CATGTATTTC CATGGTAACA GCAGAAGATC TCTTAAGCAG CAACATATCT
    TTTCCTTCAG GGAAAAAAAT AGGGGTCATC TCTCTCGAAA GTCTGCATGC ATCCACAAGG
    TCATCTGCTA GACAAGCCCT CGGCATAACC ATTGTTCGGC AGCCTGGAAG AAGAGGAACC
    GGTGACTTGC GACTAGAACC TTTCTTGTAC TTTATTGTAT CCCAGCCTTC TTTGCTTCTG
    AGTTGTCACC ACAGGAAGCA ACGAGTGGAA ATATCCATTT TTGATGCTGT GCTTAAAGGG
    GTTGCCTCTG ATTACAAATG TACAGATCCT GGGAAGACTT TGCCTGAAGC CCTTGATTAT
    TGCACAGTTT GGCTGCAGAC AGTACCTGGA GAAATAGACA GCAAAAGTGG TATTCCACCT
    TCACTCTTAA CGCTACAAAT TAAAGATTTT CTTAACGGAC CAGCAGACAT CAACTTGGAT
    ATATCAAAGC CTTTGAAAGC AAACCTGAGT TTTACCAAGT TGGATCAAAT AAACCATTTT
    TTGAAGAAGA TAAAAAATGT ACACAGCAAA GCGTCCTCAG CACCGTCAGA CGCTGTGCTG
    AGTAAGGATG CGCTTCCAGC CTCCAAATGT TACCGTGGGA AGTTATCCAA GCTTAAAGTT
    CATGGTGATG GAGCACAAAA GATTCCATTT CAAGAAAACA TATGGAGAAC GATGTCCTGC
    TTTCAAAAAA TTTCTGTTCA TACTACTCAG ATTGTGGTCT CCATGGAAAC TGTCCCCCAT
    CCTAATAAAC CATGCCTGTT GGCATCTTTA TCCACCCTCA GTGGAAGCCT TAATGTCAAA
    GCAGCGCAGA AAGTACCTGG TGTGATTCTT GGGTCATCTT TTCTACTCAG CGTAAATGAT
    TTTCTTCTTA AAACGTGTCT CAAAGAAAGA AGTCGGATTC TAATAGGACC GTGTTGTGCT
    ACTGCCAATT TGGAAGCTAA ATGGTGTAAA CACAGTGGCG ATCCAGGCCC AGAACAGTCC
    ATACCAAAAA TATCCATTGA TGTAAGAGGA GGTCTGCTAC AGGTTTTCTG GGGTCAAGAA
    CATTTGAATT GCTTAATTCT TCTACATGAA TTACTCAGTG GATACCTTAA CATGGAAGGA
    AATTTTGAAG CGAAAGTTTC TGAAGCAGTT CCACAAATGC CATCTCCTTT GGAAAAAAAT
    CAGATATTTA AAAGTGAACA AAGTTCAGAT GACCTACGGA CAGGTCTATT TCAGTATATA
    CGGGATGCTG AACCTCTGAA AATGCCTGGT GTCTATGAAG TCTTGTTTTA CAATGAAACC
    GAAGATAGCC CAGGGATGAT GCTGTGGAGA TACCCAGAAC CTAGAGTGCT CACCCTTGTA
    CGCATAACTC CTGTGCCCTT CAACACCACC GAGGATCCAG ACATTAGCAC AGCAGACCTT
    GGTGACATGC TGCAGATTCC TTGTAGTTTG GAATACTGGG ATGAACTCCA GAAGGTTTTT
    GTTGCATTTC GAGAATTTAG TCTGTCTGAA AGCAAAGTTT GTGAACTGCA ATTGCCAGAT
    ATCAATCTTG TGAACGACCA GAAGAAACTG GTATCCTCGG ATCTTTGGAG AATTGTCTTG
    AACAGCAGCC AAAATGGAGC TGATGACCAA AGCTCTGCAA GTGAATCTGG TTCTCAAAGC
    ACTTGTGACC AGCTTGTAAC CCCAACAGCT CTGGCTGCCT GTACCAGAGT TGACTCCTGC
    TTTACCCCGT GGTTTGTCCC ATCTCTTTGC ATTTCCTTCC AGTTTGCTCA CCTGGAGTTC
    CATCTTTGTC ATCACCTTGA CCAGCTTGGC ACAGCTCCAC CAAAATACCT ACAGCCATTT
    ATTTCTGATA AAAATGTGCC ATCTGAACTA GAATACATGG TTATTTCCTT CAAAGAACCA
    CACATGTATC TTCGACAGTG GAACAATAGT TCTGTCTGTC AGGAGATCCA GTTCTCAGCT
    CAAGCAGACT GTAAACTTCT AGAGTGCAGA AATGTGACAA TGCAAAGCTT GGTGAAACCC
    TTCAGCATCT TTGGACAGAC TGCAATTTCC AGCAATGCAG TAGAAAAGCT GATTGATTGC
    TCCATCATCA TTGACTCAAT ATTTGTAAAC TTTGGGCAAC ATGTAGTTCA TTCTCTCAAC
    ACTGCAGTAC AAGCTTGGCA ACAGAACAAA TGCCCTGAGG TAGAGGAGTT GGTCTTCAGC
    CATTTTGTGA TCTGTAATGA CACACAGGAG ACACTGCGGT TTGGCCAGGT GGATACTGAT
    GAAAATATCC TGCTGGCCAG TCTCTGCAGT CACCAGTACA GCTGGCGCTC TCACAAATCC
    CCACAGCTGT TACACATCTG TATTGAAGGT TGGGGCAACT GGCGTTGGTC AGAGCCCTTC
    AGTGTGGACC ATGCGGGGAC ATTTATTAGA ACTATTCAGT ACAAGGGTCG AACTGCTTCA
    CTCATCATCA AGGCTCAGCA AGTCAGCGGC GTGCAAAAAC AGATTATCAT CTGTGGAAGA
    CAGATCATCT GTAGTTACTT GTCTCAAAGC ATAGAATTAA AAGTGGTTCA GCATTACATT
    GGTCAAGATG GACAAGCTGT GGTTCGAGAA CACTTTGACT GCCTCACAGC CAAACAGAAA
    TTGCCTTCGT ACATACTAGA GAACAGCGAA CTGACTGAGT TGTGTGTGAG GGCCAAAGGA
    GATGAAGACT GGTCAAGAGA CGTGTGCCTG GAATCCAAGG CTCCTGAGTA TAGTGTCGTC
    ATTCAGGTGC CATCTTCAAA CAGTTCCATT ATTTATGTCT GGTGCACAGT TTTGACTTTA
    GAACCCAGCT CTCAAGTGCA GCAGCGAATG ATTGTGTTCA GCCCTCTTTT TATCATGAGG
    AGTCATCTTC CAGACCCCAT TATCATACAT TTGGAGAAAA GGAGTCTGGG ATTGAGTGAA
    ACACAAATTA TTCCAGGAAA AGGACAGGAA AAACCACTGC AAAACATAGA ACCTGACCTT
    GTACATCACC TAACATTTCA AGCAAGAGAA GAGGATGACC CTTCACACTG TGCCGTTCCC
    ATCTCAACAT CCCTCATTAA GCAGATAGCC ACTAAGATAC ACCCCGGAGG CACAGTTACT
    CAGATCCTTG ATGAGTTCTA CGGGCCAGAG AAGTTCCTTC AACCCACATG GCCCTATAAT
    AAAAAGGACT CTGACAGGAA TGAGCAACTG AGCCAGTGGG ACAGCCCAAT GCGAGTGAAG
    CTGTCAGCCT GGAAGCCATA TGTGAGGACC TTATTGATAG AATTGCTACC CTGGGCCCTG
    CTTATCAATC AGTCCAAGTG GGACCTCTGG CTGTTTGAGG GAGAGAAGAT TGTTCTACAG
    GTTCCTGCTG GCAAAATTAT TATCCCTCCT AATTTTCAGG AAGCTTTTCA AATTGGAATA
    TACTGGGCAA GTACAAACAC CGTGCACAAG TCAGTAGCTG TTAAGCTGGT CCATAACCTG
    ACATCTCCAA AGTGGAAAGA CGGAGGTAAC GGCGAAGTTG TATCTTTGGA CGAGGAAGGG
    TTTGTTCATG CTGAAATAAG ACTCGGTGCT TTCCCAGGAC ACCAGAAGTT GTGCCAGTTC
    TGCATTTCCT CCATGGTACA GCAAGGCATA CAGATTATTC AGATTGAAGA TAAGACCACA
    ATAATCAATA ACACACCATA TCAGATATTT TATAAGCCAC AGTTAACTGT CACCAAACTC
    CACTCTGGAA AAGAGTATTT TCATGTTCCA GACAGCGCTA CTTTCAGCAT TTGCCCGGGT
    GGCGAGCAGC CTGCCGTGAG ATCCAGCTCC CTTCCTTGCT GGGACCTAAT GCCCGACCTC
    GGCCAGTCCG CGCTGGACGC GTCCCTGCTG CAGAAGCAGG TCTTGCTGGC CTTTTCTCCC
    ACCGCCGTTG CCGACAGCGC CCAGTGCTGG AGCCTGCCAG CCCTAGTCAG ACAGGAGCTT
    CCCAGACAGA GCGTGGCAGT GCCCTTGGGG AACTACAGGG AAGATGGCTT CTGTACCAGG
    GCAATAGCAC TAACATATCA AGAACACCTT GGAGTGACTT ATTTAACCCT CTCAGAAGAC
    CCTAGTCCTC GAATAATTTT CCACAACAGA TGTCCAGTGA CAATACTTAT AAAGGAAAAT
    ACTAAAGATA CTCCGAAGTT TGAGGTTTAT TGCAGAAAAA TTCCTCCTGA GTGCTCAATT
    CATCATGAGC TATATCATCA GATTTCCAGT TATCCAGACT GCAAGACCAG AGACCTCCTT
    CCTAGCCTCC TCTTGAGAGT GGAATTGCTG GAGGAAATGA CAACCGAGTG GAGTGACGCC
    ATCGACATCA ACAATCAGGG GACACAGGTT GTGTTTCTGA CTGGCCTTGG CTATGTGTAC
    GTGGACACCA TCCATCACTG TGGCACAGTC TTCATCAACG TGGCCCCAGA AGGAAAAGCT
    GGGCCTGTCG TAACTAATAC CAACAGAACT CTGGAGAAGA TCGTGACATT TAAAATGTTC
    ATCACTCAGT TGAGCCTGGC GGTGTTTGAT GACCTCACCC ACCACAGAGC ATCATCCGAG
    CTTTTGAGAC TCACACTGGA CCACGTTTTC CTCCAGATGG CCCCCGTGGC TGGTCACCTC
    CCTGGGGAAG AGATGGCCGC CGCCCCCTTT CACGTCTACT CCGTGGAGGT CTGCTGCGGG
    GACCTGCAGC TGGACAACCA GCTGTACAAC AAATCCAATT TCCACTTTGC TGTCTTGGTC
    TGCCAGGAGG AGAAAACAGA ATCTCCTCAG TACTCCAAGA TGCAGAGTCT CCTCGTATCC
    AGTGAAGATT TGGAAGAATA CAAGAAAAAC TGCTTTATCA AACTCTGCCT CACGTTAACC
    GAAGGCAAGG ATGTCCTGTT CGATGTGAAC GAGTTCAGTT TTGAATTGAA ACCTGCCCGG
    TTATATGTGG AAGACACGTT TGTCTACTAC ATCAAAACTC TGTTTGAGAC CTACCTGCCC
    AGCAGCAGCC CGGGCGCTCA GCCCACGGCT CTCGCAGGCG GCAGACAGGT GCTGCCCATG
    CAGGTGAGGC AGCACGCCCG GGCCTTGGTG CATCCCGTGA AGCTGCAGAA GCTGGTGATA
    CAGCCTGTGA ACCTCCTCGT CAGCATCCAT GCGTCCCTCA AGCTGTACAT CGCCTCTGAC
    CACACTCCGC TCTCCTTCTC AGTGTTTGAA AGAGGCCCCA TCTTCACCAC GGCCAGGCAG
    CTCGTCCACG CCCTGGCCAT GCACTACGCC GCAGGGGCCC TCTTCAGAGC AGGCTGGGTG
    GTTGGATCCC TGGAAATCCT TGGCAGCCCG GCGAGTCTGG TGCGAAGCAT CGGGAACGGC
    ATCGCTGACT TCTTCAGGCT TCCGTACGAG GGGCTGACCC GAGGCCCGGG AGCCTTCGTG
    AGCGGGGTTT CCAGAGGGAC TACATCGTTT GTGAAGCACA TTTCCAAAGG TACCCTCACG
    TCCATCACCA ACCTGGCCAC GAGCCTGGCC CGGAACATGG ACCGGCTGTC GCTGGACGAG
    GAGCACTACA ACCGGCAGGA AGAGTGGCGG CGGCAGCTCC CGGAGAGCCT GGGCGAGGGG
    CTCCGGCAGG GCCTGTCCCG GCTGGGTATC AGTCTGCTCG GTGCAATTGC TGGTATAGTT
    GATCAGCCAA TGCAGAACTT CCAGAAAACA TCAGAGGCTC AAGCTTCAGC AGGACACAAG
    GCCAAGGGTG TTATCTCCGG CGTGGGGAAA GGAATCATGG GGGTGTTCAC GAAGCCCATC
    GGAGGGGCTG CTGAGCTGGT GTCACAGACT GGCTATGGTA TTTTACACGG AGCTGGACTT
    TCTCAGCTTC CCAAACAGCG CTATCAGCCA AGTGATCTGC ACGCTGACCA GGCTCCAAAC
    AGCCACGTCA AATACGTCTG GAAGATGCTC CAGTCTCTGG GCAGACCGGA AGTACACATG
    GCCCTGGAGG TTGTTCTGGT GAGGGGCTCG GGCCAGGAGC ACGAGGGCTG CCTGCTGCTG
    ACGTCAGACG TGCTCTTCGT GGTGAGCGTC AGCGAGGACA CCCAGCAGCA GGCCTTCCCA
    GTCACGGAGA TCGACTGCGC ACAGGACCGC GAGCAGAGCA GCCTGCTCAT CGTGCAGCTC
    AAGCAGCCCA GAGTGGCCTG TGACGCAGAG GTGGATGGAG TTCGAGAGAG ACTGTCAGAG
    CAACAGTATA ACAGACTTGT GGACTACATC ACAAAGACCT CTTGTCACCT GGGCCCCGGC
    TGCTCCTCCG TGCAGGCGTC ATGCCCTGTG GTGGCTGCGG AACCGCTCCC CTCCACTGTG
    AAAACATACC ATTATATGGT TGATCCACAT TTTGCCCAAG TTTTCATTAG TAAATTTACC
    ATGGTGAAAA ATAAAGCCCT GAGGAAAGGG TTCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120554.1
    CDS1..11979
    Translation

    Target ORF information:

    RefSeq Version XM_025264769.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAAGCCAGG GGAAGGTTGG CAGTCAGGAC ATTTTGAAGG AGTATTTCTA
    CAGTGCAAAG AAAAACCTGT GACAACTGCA AAGCTTCTAG ATGGCTCTCA TCAGCAGCAT
    GGATTTCTGT CTTTGACATA CACAAAAGCT GTAACAAAAA ATGTTCGCCA CAAGTTAACA
    TCAAGAAATG AGCGAAGAAG TTTTCATAAG TTATCTGAAG GTTTAACAGA TGGCTCCCCT
    CATTTTCTTC ATGAAATACT TCTTTCAGCA CAAGCCTTTG ATATTGTCCT TTGTTTTCCT
    TTACTTAATG CCATTGCAAG TATATTTCAA GCAAAACTAC CAAGGACCCA AAAAGAGAAA
    AGAAAATCTC CTGGTCAGCC CATGAGGACC CATACACTGA CTTCACGCAA TTTACCTTTG
    ATTTATATCA ACACAAGCGT AATCAGAATT TTTGTTCCAA AAACAGAAGA AATGCAGCCA
    AGTATTGAAG TTAATCAGAC AGCAAAGGAA GATACCATGG TTCTGAAGAT TGGCTCTGTT
    GCTATGGCTC CCCAGGCTGA CAATCCCCTT GGCAGATCCG TCCTCAGGAA AGATATTTAC
    CAAAGAGCCT TGAATTTAGG AATTCTTCGA GACCCTGGGT CAGAGATTGA AGACCGACAA
    TACCAAATAG ATCTGCAGTC GATCAATATT GGTACTGCTC AGTGGGATCA ACTGAAACCA
    GAAAAGGAAA ATGGCACAGG AGGAGTGCTA ACAGAGACTG AAAGAAATTC GCAGAATCCA
    GCCCTTGAGT GGAATATGGC CAGCAGCATA CGGCGGCATC AAGAAAGAAG AGCAATTTTG
    ACTCCCATTT TAACAGATTT TTCTGTACGA ATAACTGGAG CACCTGCCAT CATTTTCACC
    AAAATAATTT CTCCGGAAAA TATGCACACT GAGGAGATTT TAGTGTGTGG CCATTCCTTG
    GAAGTGAATA TAACCACAAA CCTGGACTTC TTCCTAAGTG TGGCTCAAGT TCAACTCTTA
    CATCAGTTAA TAGTAGCAAA TATGACTGGA CTGGAACCAT CAAACAAAGC CACAGAGATC
    GCTAAGCAAG AACAGAAAAA AATGGATACA TATGATGGAA TCGTGGCTGA TAGCTCATCT
    CGGTACAGTG GTGCCCAGGA TAGTGGAATC GGCAGTGACA GCGTTAAAAT CAGAATAGTA
    CAGATAGAGC AGCACAGCGG TGCCAGTCAG CACCGCATCG CCCGTCCCTC ACGCCAGTCG
    TCAATTGTAA AAAATCTAAA TTTTATTCCT TTTGACATAT TTATTACTGC AAGTAGAATC
    TCACTAATGA CCTATTCCTG TACGGCCTTA CCCAAATCGA AATTGCAAGA ACAAAAGGAT
    AATGAAAAAA CAGGCAAGAG TTCATTAAAC CTGCCAGAAG TCGATTCAGA TGTCGCAAAA
    CCCAGCCAGG CATGTATTTC CATGGTAACA GCAGAAGATC TCTTAAGCAG CAACATATCT
    TTTCCTTCAG GGAAAAAAAT AGGGGTCATC TCTCTCGAAA GTCTGCATGC ATCCACAAGG
    TCATCTGCTA GACAAGCCCT CGGCATAACC ATTGTTCGGC AGCCTGGAAG AAGAGGAACC
    GGTGACTTGC GACTAGAACC TTTCTTGTAC TTTATTGTAT CCCAGCCTTC TTTGCTTCTG
    AGTTGTCACC ACAGGAAGCA ACGAGTGGAA ATATCCATTT TTGATGCTGT GCTTAAAGGG
    GTTGCCTCTG ATTACAAATG TACAGATCCT GGGAAGACTT TGCCTGAAGC CCTTGATTAT
    TGCACAGTTT GGCTGCAGAC AGTACCTGGA GAAATAGACA GCAAAAGTGG TATTCCACCT
    TCACTCTTAA CGCTACAAAT TAAAGATTTT CTTAACGGAC CAGCAGACAT CAACTTGGAT
    ATATCAAAGC CTTTGAAAGC AAACCTGAGT TTTACCAAGT TGGATCAAAT AAACCATTTT
    TTGAAGAAGA TAAAAAATGT ACACAGCAAA GCGTCCTCAG CACCGTCAGA CGCTGTGCTG
    AGTAAGGATG CGCTTCCAGC CTCCAAATGT TACCGTGGGA AGTTATCCAA GCTTAAAGTT
    CATGGTGATG GAGCACAAAA GATTCCATTT CAAGAAAACA TATGGAGAAC GATGTCCTGC
    TTTCAAAAAA TTTCTGTTCA TACTACTCAG ATTGTGGTCT CCATGGAAAC TGTCCCCCAT
    CCTAATAAAC CATGCCTGTT GGCATCTTTA TCCACCCTCA GTGGAAGCCT TAATGTCAAA
    GCAGCGCAGA AAGTACCTGG TGTGATTCTT GGGTCATCTT TTCTACTCAG CGTAAATGAT
    TTTCTTCTTA AAACGTGTCT CAAAGAAAGA AGTCGGATTC TAATAGGACC GTGTTGTGCT
    ACTGCCAATT TGGAAGCTAA ATGGTGTAAA CACAGTGGCG ATCCAGGCCC AGAACAGTCC
    ATACCAAAAA TATCCATTGA TGTAAGAGGA GGTCTGCTAC AGGTTTTCTG GGGTCAAGAA
    CATTTGAATT GCTTAATTCT TCTACATGAA TTACTCAGTG GATACCTTAA CATGGAAGGA
    AATTTTGAAG CGAAAGTTTC TGAAGCAGTT CCACAAATGC CATCTCCTTT GGAAAAAAAT
    CAGATATTTA AAAGTGAACA AAGTTCAGAT GACCTACGGA CAGGTCTATT TCAGTATATA
    CGGGATGCTG AACCTCTGAA AATGCCTGGT GTCTATGAAG TCTTGTTTTA CAATGAAACC
    GAAGATAGCC CAGGGATGAT GCTGTGGAGA TACCCAGAAC CTAGAGTGCT CACCCTTGTA
    CGCATAACTC CTGTGCCCTT CAACACCACC GAGGATCCAG ACATTAGCAC AGCAGACCTT
    GGTGACATGC TGCAGATTCC TTGTAGTTTG GAATACTGGG ATGAACTCCA GAAGGTTTTT
    GTTGCATTTC GAGAATTTAG TCTGTCTGAA AGCAAAGTTT GTGAACTGCA ATTGCCAGAT
    ATCAATCTTG TGAACGACCA GAAGAAACTG GTATCCTCGG ATCTTTGGAG AATTGTCTTG
    AACAGCAGCC AAAATGGAGC TGATGACCAA AGCTCTGCAA GTGAATCTGG TTCTCAAAGC
    ACTTGTGACC AGCTTGTAAC CCCAACAGCT CTGGCTGCCT GTACCAGAGT TGACTCCTGC
    TTTACCCCGT GGTTTGTCCC ATCTCTTTGC ATTTCCTTCC AGTTTGCTCA CCTGGAGTTC
    CATCTTTGTC ATCACCTTGA CCAGCTTGGC ACAGCTCCAC CAAAATACCT ACAGCCATTT
    ATTTCTGATA AAAATGTGCC ATCTGAACTA GAATACATGG TTATTTCCTT CAAAGAACCA
    CACATGTATC TTCGACAGTG GAACAATAGT TCTGTCTGTC AGGAGATCCA GTTCTCAGCT
    CAAGCAGACT GTAAACTTCT AGAGTGCAGA AATGTGACAA TGCAAAGCTT GGTGAAACCC
    TTCAGCATCT TTGGACAGAC TGCAATTTCC AGCAATGCAG TAGAAAAGCT GATTGATTGC
    TCCATCATCA TTGACTCAAT ATTTGTAAAC TTTGGGCAAC ATGTAGTTCA TTCTCTCAAC
    ACTGCAGTAC AAGCTTGGCA ACAGAACAAA TGCCCTGAGG TAGAGGAGTT GGTCTTCAGC
    CATTTTGTGA TCTGTAATGA CACACAGGAG ACACTGCGGT TTGGCCAGGT GGATACTGAT
    GAAAATATCC TGCTGGCCAG TCTCTGCAGT CACCAGTACA GCTGGCGCTC TCACAAATCC
    CCACAGCTGT TACACATCTG TATTGAAGGT TGGGGCAACT GGCGTTGGTC AGAGCCCTTC
    AGTGTGGACC ATGCGGGGAC ATTTATTAGA ACTATTCAGT ACAAGGGTCG AACTGCTTCA
    CTCATCATCA AGGCTCAGCA AGTCAGCGGC GTGCAAAAAC AGATTATCAT CTGTGGAAGA
    CAGATCATCT GTAGTTACTT GTCTCAAAGC ATAGAATTAA AAGTGGTTCA GCATTACATT
    GGTCAAGATG GACAAGCTGT GGTTCGAGAA CACTTTGACT GCCTCACAGC CAAACAGAAA
    TTGCCTTCGT ACATACTAGA GAACAGCGAA CTGACTGAGT TGTGTGTGAG GGCCAAAGGA
    GATGAAGACT GGTCAAGAGA CGTGTGCCTG GAATCCAAGG CTCCTGAGTA TAGTGTCGTC
    ATTCAGGTGC CATCTTCAAA CAGTTCCATT ATTTATGTCT GGTGCACAGT TTTGACTTTA
    GAACCCAGCT CTCAAGTGCA GCAGCGAATG ATTGTGTTCA GCCCTCTTTT TATCATGAGG
    AGTCATCTTC CAGACCCCAT TATCATACAT TTGGAGAAAA GGAGTCTGGG ATTGAGTGAA
    ACACAAATTA TTCCAGGAAA AGGACAGGAA AAACCACTGC AAAACATAGA ACCTGACCTT
    GTACATCACC TAACATTTCA AGCAAGAGAA GAGGATGACC CTTCACACTG TGCCGTTCCC
    ATCTCAACAT CCCTCATTAA GCAGATAGCC ACTAAGATAC ACCCCGGAGG CACAGTTACT
    CAGATCCTTG ATGAGTTCTA CGGGCCAGAG AAGTTCCTTC AACCCACATG GCCCTATAAT
    AAAAAGGACT CTGACAGGAA TGAGCAACTG AGCCAGTGGG ACAGCCCAAT GCGAGTGAAG
    CTGTCAGCCT GGAAGCCATA TGTGAGGACC TTATTGATAG AATTGCTACC CTGGGCCCTG
    CTTATCAATC AGTCCAAGTG GGACCTCTGG CTGTTTGAGG GAGAGAAGAT TGTTCTACAG
    GTTCCTGCTG GCAAAATTAT TATCCCTCCT AATTTTCAGG AAGCTTTTCA AATTGGAATA
    TACTGGGCAA GTACAAACAC CGTGCACAAG TCAGTAGCTG TTAAGCTGGT CCATAACCTG
    ACATCTCCAA AGTGGAAAGA CGGAGGTAAC GGCGAAGTTG TATCTTTGGA CGAGGAAGGG
    TTTGTTCATG CTGAAATAAG ACTCGGTGCT TTCCCAGGAC ACCAGAAGTT GTGCCAGTTC
    TGCATTTCCT CCATGGTACA GCAAGGCATA CAGATTATTC AGATTGAAGA TAAGACCACA
    ATAATCAATA ACACACCATA TCAGATATTT TATAAGCCAC AGTTAACTGT CACCAAACTC
    CACTCTGGAA AAGAGTATTT TCATGTTCCA GACAGCGCTA CTTTCAGCAT TTGCCCGGGT
    GGCGAGCAGC CTGCCGTGAG ATCCAGCTCC CTTCCTTGCT GGGACCTAAT GCCCGACCTC
    GGCCAGTCCG CGCTGGACGC GTCCCTGCTG CAGAAGCAGG TCTTGCTGGC CTTTTCTCCC
    ACCGCCGTTG CCGACAGCGC CCAGTGCTGG AGCCTGCCAG CCCTAGTCAG ACAGGAGCTT
    CCCAGACAGA GCGTGGCAGT GCCCTTGGGG AACTACAGGG AAGATGGCTT CTGTACCAGG
    GCAATAGCAC TAACATATCA AGAACACCTT GGAGTGACTT ATTTAACCCT CTCAGAAGAC
    CCTAGTCCTC GAATAATTTT CCACAACAGA TGTCCAGTGA CAATACTTAT AAAGGAAAAT
    ACTAAAGATA CTCCGAAGTT TGAGGTTTAT TGCAGAAAAA TTCCTCCTGA GTGCTCAATT
    CATCATGAGC TATATCATCA GATTTCCAGT TATCCAGACT GCAAGACCAG AGACCTCCTT
    CCTAGCCTCC TCTTGAGAGT GGAATTGCTG GAGGAAATGA CAACCGAGTG GAGTGACGCC
    ATCGACATCA ACAATCAGGG GACACAGGTT GTGTTTCTGA CTGGCCTTGG CTATGTGTAC
    GTGGACACCA TCCATCACTG TGGCACAGTC TTCATCAACG TGGCCCCAGA AGGAAAAGCT
    GGGCCTGTCG TAACTAATAC CAACAGAACT CTGGAGAAGA TCGTGACATT TAAAATGTTC
    ATCACTCAGT TGAGCCTGGC GGTGTTTGAT GACCTCACCC ACCACAGAGC ATCATCCGAG
    CTTTTGAGAC TCACACTGGA CCACGTTTTC CTCCAGATGG CCCCCGTGGC TGGTCACCTC
    CCTGGGGAAG AGATGGCCGC CGCCCCCTTT CACGTCTACT CCGTGGAGGT CTGCTGCGGG
    GACCTGCAGC TGGACAACCA GCTGTACAAC AAATCCAATT TCCACTTTGC TGTCTTGGTC
    TGCCAGGAGG AGAAAACAGA ATCTCCTCAG TACTCCAAGA TGCAGAGTCT CCTCGTATCC
    AGTGAAGATT TGGAAGAATA CAAGAAAAAC TGCTTTATCA AACTCTGCCT CACGTTAACC
    GAAGGCAAGG ATGTCCTGTT CGATGTGAAC GAGTTCAGTT TTGAATTGAA ACCTGCCCGG
    TTATATGTGG AAGACACGTT TGTCTACTAC ATCAAAACTC TGTTTGAGAC CTACCTGCCC
    AGCAGCAGCC CGGGCGCTCA GCCCACGGCT CTCGCAGGCG GCAGACAGGT GCTGCCCATG
    CAGGTGAGGC AGCACGCCCG GGCCTTGGTG CATCCCGTGA AGCTGCAGAA GCTGGTGATA
    CAGCCTGTGA ACCTCCTCGT CAGCATCCAT GCGTCCCTCA AGCTGTACAT CGCCTCTGAC
    CACACTCCGC TCTCCTTCTC AGTGTTTGAA AGAGGCCCCA TCTTCACCAC GGCCAGGCAG
    CTCGTCCACG CCCTGGCCAT GCACTACGCC GCAGGGGCCC TCTTCAGAGC AGGCTGGGTG
    GTTGGATCCC TGGAAATCCT TGGCAGCCCG GCGAGTCTGG TGCGAAGCAT CGGGAACGGC
    ATCGCTGACT TCTTCAGGCT TCCGTACGAG GGGCTGACCC GAGGCCCGGG AGCCTTCGTG
    AGCGGGGTTT CCAGAGGGAC TACATCGTTT GTGAAGCACA TTTCCAAAGG TACCCTCACG
    TCCATCACCA ACCTGGCCAC GAGCCTGGCC CGGAACATGG ACCGGCTGTC GCTGGACGAG
    GAGCACTACA ACCGGCAGGA AGAGTGGCGG CGGCAGCTCC CGGAGAGCCT GGGCGAGGGG
    CTCCGGCAGG GCCTGTCCCG GCTGGGTATC AGTCTGCTCG GTGCAATTGC TGGTATAGTT
    GATCAGCCAA TGCAGAACTT CCAGAAAACA TCAGAGGCTC AAGCTTCAGC AGGACACAAG
    GCCAAGGGTG TTATCTCCGG CGTGGGGAAA GGAATCATGG GGGTGTTCAC GAAGCCCATC
    GGAGGGGCTG CTGAGCTGGT GTCACAGACT GGCTATGGTA TTTTACACGG AGCTGGACTT
    TCTCAGCTTC CCAAACAGCG CTATCAGCCA AGTGATCTGC ACGCTGACCA GGCTCCAAAC
    AGCCACGTCA AATACGTCTG GAAGATGCTC CAGTCTCTGG GCAGACCGGA AGTACACATG
    GCCCTGGAGG TTGTTCTGGT GAGGGGCTCG GGCCAGGAGC ACGAGGGCTG CCTGCTGCTG
    ACGTCAGACG TGCTCTTCGT GGTGAGCGTC AGCGAGGACA CCCAGCAGCA GGCCTTCCCA
    GTCACGGAGA TCGACTGCGC ACAGGACCGC GAGCAGAGCA GCCTGCTCAT CGTGCAGCTC
    AAGCAGCCCA GAGTGGCCTG TGACGCAGAG GTGGATGGAG TTCGAGAGAG ACTGTCAGAG
    CAACAGTATA ACAGACTTGT GGACTACATC ACAAAGACCT CTTGTCACCT GGGCCCCGGC
    TGCTCCTCCG TGCAGGCGTC ATGCCCTGTG GTGGCTGCGG AACCGCTCCC CTCCACTGTG
    AAAACATACC ATTATATGGT TGATCCACAT TTTGCCCAAG TTTTCATTAG TAAATTTACC
    ATGGTGAAAA ATAAAGCCCT GAGGAAAGGG TTCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49001
    Clone ID Related Accession (Same CDS sequence) XM_025264770.1
    Accession Version XM_025264770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAT GATCATGAAA GCTGTGGTTC TAATTCTACC
    AACCGTAGTA CTACTGAGAA CACAAAAACA TCAGCAAAAC CCCGAAGAAT TCAACAGGCC
    ACTCCCACAG ATCCTGATTT GCCACCAGGC TATGTACAGA GTCTGATCAG ACGAGTTGTA
    AATAATGTAA ATATTGTTAT AAATAATCTG ATACTAAAAT ATGTTGAAGA TGATATTGTC
    CTTTCAGTCA ACATCACATC CGCAGAATGC TATACAGTAG GTGAATTGTG GGATCGTGCA
    TTCATGGATA TTTCTGCAAC TGATCTGGTG TTGAGAAAGG TTATCAATTT TTCTGACTGT
    ACAGTTTGTC TTGATAAACG AAATGCCAGT GGTAAAATAG AATTCTACCA GGATCCTTTA
    TTGTACAAGT GTTCCTTCAG AACTCGTCTG CATTTTACTT ACGATAACCT AAATTCTAAG
    ATGCCATCAG TTATAAAAAT TCATACCTTA GTAGAGAGTT TGAAACTTTC TATCACTGAT
    CAGCAGTTGC CTATGTTTAT CCGTATAATG CAACTTGGAA TTGCTCTTTA CTATGGAGAA
    ATAGGAAATT TTAAAGATGG AGAAACCGAA GATTCTACTT GTCACAATAA AGATATGTTG
    GGAAATGTTA CAGGTGCTGA AGATGAAACA CGAATAGATA TGCAGTATCC TGCTCAGTAC
    AAAGGCCAAG AGCTGTATTC ACAGCAAGAG GACGACCAGC CACAGGGATG GGTATCATGG
    GCCTGGTCAT TTGTCCCTGC CATTGTGAAT TATGATGATG CCGAGGAAGA CTACCTTGGG
    AATGATCCTA CATCAACTAC ACATCAACAA AAAACACAGA CTTTGAAGGA TCCTATTGTT
    TCTATAGGAT TTTATTGTAC AAAGGCAACT GTGACTTTCA AACTTACTGA AATGCAAGCC
    GAGAGTAGTT ACTACAGCCC TCAAAAAGTA AAATCTAAAG AAGTATTATG TTGGGAACAA
    GAAGGAACTA CAGTTGAGGC CCTTATGATG GGAGAGCCTT TCTTTGATTG CCAGGTTGGT
    TTTGTAGGTT GCAGAGCCAT GTGCCTTAAA GGAATTATGG GTGTTAAAGA TTTTGAAGAG
    AATATGAATA GAAGTGAAAC CGAAGCCTGT TTCTTCCTTT GTGGTGAAAA TTTGAGTATA
    AAAGGTTTGA CATACCTCAC AAATTCATTG TTTGATTACC GAAGCCCAGA AAATAATGGT
    ACTCGTGCAG AATTTATCTT GGATGCAGCT ACTCATAAGG AGACATACAC TGAGATAGCT
    GGAATGCAAA GGTTCGGGGC ATTTTACATG GATTACCTGT ATACAATGGA GAACACCACT
    GGCAAAGGTT CTGCAAATCA ACAAGACTTT TCTTCAGGGA AGAGTGAAGA TTTGGGAACA
    GTTCAGGAAA AGTCTACCAA AAGCCTTGTT ATAGGTCCTC TTGATTTTCG CTTGGATAGC
    AGTGCAGTGC ACAGAATTTT AAAAATGGTT GTTTGTGCCT TGGAACATGA ATATGAACCA
    TATAGTAGAC TAAAACCAGA TATTAAAGAT GAAAGTGAAA CAATGCTGAA TCCTGAGGAA
    GTGGCATCTT TGGAGGAATA TATTCCTACT CGACATACAA GTGTTACTCT CCTCAAATGT
    ACCTTCACAA TTTTCATGGC TGAATTCAAT TTGCTGGATC ATTTGCTACC TGTTATTATG
    GGGGAAAAGA ACTCAAGTAA CTTCATGAAT ACTACAAACT TCCAGTCTCT CCGGCCATTG
    CCTTCTGTTC AGATATTGGT GGATAAAATT AATCTGGAAC ATTCTGTGCC GATGTATGCT
    GAACAGTTGG TGCATGTGGT CAGCAGTCTT ACTCAGCCTT CTGATAATCT GCTTCATTAT
    TGCTATGCAC ATTGCTACCT TAAGATATTT GGTTTCCAGG CAGGACTGAC GTCTTTGGAT
    TGTAGGGGAT CTTACTGCTT ACCTGTACCA ATTGTTCCCT CTTTCAGCAC TGCTCTTTAT
    GGTAAACTTC TGAAACTACC CACATGCTGG ACCAAAAGAT CTCAGATTGC TATAACTGAA
    GGCATTTTTG AGCTTCCAAA TCTTACAGTT CAAGCTACAA GAGCTCAGAC ACTTCTGCTT
    CAAGCAATAT ATCAAAGTTG GTCTCATAGT GGAAATGTCA GCTCTTTAGC AGTTAATGAA
    GCTTTGATGA ATGAAGTTTT CCAGACTGTA GGTGTGAAAT CTAAGAATCC CCTGCCAACT
    CTTGAGGGCT CAATCCAGAA TGTTGAATTG AAGTACTGCA GCACATCATT GGTCAAATGT
    GCCTCTGGGA CCGTGGGATC AATAAAAATT TGTGCCAAAG CCCCAGGTGA CAGTGGAAAA
    GAGAAGTTGA TTCCATTGCT TCAGGGTCCT TCTGACACTA AAGACCTTCA TAGCAGCAAG
    TGGCTCAATG AGAGCAGAAA GCCAGAATCT CTCTTAGCTC CAGATTTGAT AGCCTTCACA
    GTCCAGGTTC CACAGTGTAT GGACTACTGT CACAGTTCCG GTGCCGTACT CCTTTGTAAC
    GTCCAAGGAC TAGCAGTTAA TATTGACCCT ATCTTATACA CCTGGCTCAT TTATCAACCT
    CAGAAGCGAA GCAGTAAACA TATGCAGCAG CCTGTAATTG CTGTTCCTCT TGTTATGCCA
    ATTAGCAGAA GGAAAGAGGA TGAGGTGTCT GTTGGAAGTG CACCCTTGGC AAAGCAACAG
    TCATATCAGG CATCTGAGTA TGCCAGCAGC CCTGTAAAAA CAAAAACTGT AACAGAATCC
    AGACCATTGT CAGTTCCTGT GAAAGCTGTG CTGACGATAA CTGAAGGCTG TAGAAGTCCT
    GAAGAAAGAA TGAAAGAGTT TATTGGAATA GTATGGAATG CAGTTAAGAG TCTAACACTG
    CAGCTTGAAG TACAGTCTTG TTGTGTGTTT ATCCCAAATG ATAGTTTGCC TTCTCCTAGT
    ACAATTGTAT CTGGTGACAT TCCTGGAACA GTAAGAAGTT GGTACCATGG ACAAATGAGC
    ATGCCAGGAA CGCTTGTCCT CTGTTTGCCT CAAATAAAGA TTGTTAGTGC TGGCCACAAG
    TATATGGACC CTCTCCAGGA GATTCCGTTT GTCATCCCAC GACCCATCCT TGAAGAAGGT
    GATGCTTTTC CTTGGACAAT CAGCTTGCAT CATTTCAGCA TTTATACCCT TTTTGGAAAA
    CAAGTGACAC TTTGCCTAGT GGAACCCATG GGTTGTACCT CTACACTAGC TGTTACATCC
    CAAAAATTGC TTGCTACAGG ACCTGATATG AGGCATTCAT TTGTTGTCTG TCTGCATGTT
    GACCTAGAGT CACTTGAGAT AAAATGCTCA AACCCCCAGG TTCAGCTCTT ATATGAGTTA
    ACAGATATCA TGAATGAAGT CTGGAATAAG ATTCAGAAGA GAGGCAATCT CAGTTCTTCT
    TCAGCTTACC CGGAGTCCGT GGTGGGGCCT TTCCCTAGCT CTCCAGTTAG AAGCAGTATA
    GGCACAGCTC CTCCAGATAA TAGCACATGT AGCCCATCTG CTGACATTGG AACTACTACT
    GAGGGAGATT CTGTACAAGC TGTTGATGAT TCACCATTCT CAGATTCTGT TACCTTGGAA
    CAGACTACAA GTAACATTGG TGGATCCAGT GGTCGTGTTA GTCTGTGGAT GCAGTGGGTG
    CTCCCCAAAA TTACTGTAAA ACTCTTTGCT CCAGATCCTG ACAATAAAGG CATAGAACTT
    TGTATGGTCA GTGAACTAGA AGATCTCAGT GCTTCAATAG ATGTCCAGGA TGTGTACACT
    AAAGTGAAAT GTAAAATAGA AAGTTTCAAT ATTGATCACT ATAGAAGCAA CCTTGGGGAA
    GAGTGTTGGT CTCTGGGGCA ATGTGGAGGT GTCTTCCTTT CCTGTACTGA CAAGCTGAAC
    AGACGCACCT TGTTGGTTCG ACCCATCAGC AAGCAGGACC CTTTCAGTAA TTGCTCTGGC
    TTCTTTCCTT CTACAACTGC AAAGCTTCTA GATGGCTCTC ATCAGCAGCA TGGATTTCTG
    TCTTTGACAT ACACAAAAGC TGTAACAAAA AATGTTCGCC ACAAGTTAAC ATCAAGAAAT
    GAGCGAAGAA GTTTTCATAA GTTATCTGAA GGTTTAACAG ATGGCTCCCC TCATTTTCTT
    CATGAAATAC TTCTTTCAGC ACAAGCCTTT GATATTGTCC TTTGTTTTCC TTTACTTAAT
    GCCATTGCAA GTATATTTCA AGCAAAACTA CCAAGGACCC AAAAAGAGAA AAGAAAATCT
    CCTGGTCAGC CCATGAGGAC CCATACACTG ACTTCACGCA ATTTACCTTT GATTTATATC
    AACACAAGCG TAATCAGAAT TTTTGTTCCA AAAACAGAAG AAATGCAGCC AAGTATTGAA
    GTTAATCAGA CAGCAAAGGA AGATACCATG GTTCTGAAGA TTGGCTCTGT TGCTATGGCT
    CCCCAGGCTG ACAATCCCCT TGGCAGATCC GTCCTCAGGA AAGATATTTA CCAAAGAGCC
    TTGAATTTAG GAATTCTTCG AGACCCTGGG TCAGAGATTG AAGACCGACA ATACCAAATA
    GATCTGCAGT CGATCAATAT TGGTACTGCT CAGTGGGATC AACTGAAACC AGAAAAGGAA
    AATGGCACAG GAGGAGTGCT AACAGAGACT GAAAGAAATT CGCAGAATCC AGCCCTTGAG
    TGGAATATGG CCAGCAGCAT ACGGCGGCAT CAAGAAAGAA GAGCAATTTT GACTCCCATT
    TTAACAGATT TTTCTGTACG AATAACTGGA GCACCTGCCA TCATTTTCAC CAAAATAATT
    TCTCCGGAAA ATATGCACAC TGAGGAGATT TTAGTGTGTG GCCATTCCTT GGAAGTGAAT
    ATAACCACAA ACCTGGACTT CTTCCTAAGT GTGGCTCAAG TTCAACTCTT ACATCAGTTA
    ATAGTAGCAA ATATGACTGG ACTGGAACCA TCAAACAAAG CCACAGAGAT CGCTAAGCAA
    GAACAGAAAA AAATGGATAC ATATGATGGA ATCGTGGCTG ATAGCTCATC TCGGTACAGT
    GGTGCCCAGG ATAGTGGAAT CGGCAGTGAC AGCGTTAAAA TCAGAATAGT ACAGATAGAG
    CAGCACAGCG GTGCCAGTCA GCACCGCATC GCCCGTCCCT CACGCCAGTC GTCAATTGTA
    AAAAATCTAA ATTTTATTCC TTTTGACATA TTTATTACTG CAAGTAGAAT CTCACTAATG
    ACCTATTCCT GTACGGCCTT ACCCAAATCG AAATTGCAAG AACAAAAGGA TAATGAAAAA
    ACAGGCAAGA GTTCATTAAA CCTGCCAGAA GTCGATTCAG ATGTCGCAAA ACCCAGCCAG
    GCATGTATTT CCATGGTAAC AGCAGAAGAT CTCTTAAGCA GCAACATATC TTTTCCTTCA
    GGGAAAAAAA TAGGGGTCAT CTCTCTCGAA AGTCTGCATG CATCCACAAG GTCATCTGCT
    AGACAAGCCC TCGGCATAAC CATTGTTCGG CAGCCTGGAA GAAGAGGAAC CGGTGACTTG
    CGACTAGAAC CTTTCTTGTA CTTTATTGTA TCCCAGCCTT CTTTGCTTCT GAGTTGTCAC
    CACAGGAAGC AACGAGTGGA AATATCCATT TTTGATGCTG TGCTTAAAGG GGTTGCCTCT
    GATTACAAAT GTACAGATCC TGGGAAGACT TTGCCTGAAG CCCTTGATTA TTGCACAGTT
    TGGCTGCAGA CAGTACCTGG AGAAATAGAC AGCAAAAGTG GTATTCCACC TTCACTCTTA
    ACGCTACAAA TTAAAGATTT TCTTAACGGA CCAGCAGACA TCAACTTGGA TATATCAAAG
    CCTTTGAAAG CAAACCTGAG TTTTACCAAG TTGGATCAAA TAAACCATTT TTTGAAGAAG
    ATAAAAAATG TACACAGCAA AGCGTCCTCA GCACCGTCAG ACGCTGTGCT GAGTAAGGAT
    GCGCTTCCAG CCTCCAAATG TTACCGTGGG AAGTTATCCA AGCTTAAAGT TCATGGTGAT
    GGAGCACAAA AGATTCCATT TCAAGAAAAC ATATGGAGAA CGATGTCCTG CTTTCAAAAA
    ATTTCTGTTC ATACTACTCA GATTGTGGTC TCCATGGAAA CTGTCCCCCA TCCTAATAAA
    CCATGCCTGT TGGCATCTTT ATCCACCCTC AGTGGAAGCC TTAATGTCAA AGCAGCGCAG
    AAAGTACCTG GTGTGATTCT TGGGTCATCT TTTCTACTCA GCGTAAATGA TTTTCTTCTT
    AAAACGTGTC TCAAAGAAAG AAGTCGGATT CTAATAGGAC CGTGTTGTGC TACTGCCAAT
    TTGGAAGCTA AATGGTGTAA ACACAGTGGC GATCCAGGCC CAGAACAGTC CATACCAAAA
    ATATCCATTG ATGTAAGAGG AGGTCTGCTA CAGGTTTTCT GGGGTCAAGA ACATTTGAAT
    TGCTTAATTC TTCTACATGA ATTACTCAGT GGATACCTTA ACATGGAAGG AAATTTTGAA
    GCGAAAGTTT CTGAAGCAGT TCCACAAATG CCATCTCCTT TGGAAAAAAA TCAGATATTT
    AAAAGTGAAC AAAGTTCAGA TGACCTACGG ACAGGTCTAT TTCAGTATAT ACGGGATGCT
    GAACCTCTGA AAATGCCTGG TGTCTATGAA GTCTTGTTTT ACAATGAAAC CGAAGATAGC
    CCAGGGATGA TGCTGTGGAG ATACCCAGAA CCTAGAGTGC TCACCCTTGT ACGCATAACT
    CCTGTGCCCT TCAACACCAC CGAGGATCCA GACATTAGCA CAGCAGACCT TGGTGACATG
    CTGCAGATTC CTTGTAGTTT GGAATACTGG GATGAACTCC AGAAGGTTTT TGTTGCATTT
    CGAGAATTTA GTCTGTCTGA AAGCAAAGTT TGTGAACTGC AATTGCCAGA TATCAATCTT
    GTGAACGACC AGAAGAAACT GGTATCCTCG GATCTTTGGA GAATTGTCTT GAACAGCAGC
    CAAAATGGAG CTGATGACCA AAGCTCTGCA AGTGAATCTG GTTCTCAAAG CACTTGTGAC
    CAGCTTGTAA CCCCAACAGC TCTGGCTGCC TGTACCAGAG TTGACTCCTG CTTTACCCCG
    TGGTTTGTCC CATCTCTTTG CATTTCCTTC CAGTTTGCTC ACCTGGAGTT CCATCTTTGT
    CATCACCTTG ACCAGCTTGG CACAGCTCCA CCAAAATACC TACAGCCATT TATTTCTGAT
    AAAAATGTGC CATCTGAACT AGAATACATG GTTATTTCCT TCAAAGAACC ACACATGTAT
    CTTCGACAGT GGAACAATAG TTCTGTCTGT CAGGAGATCC AGTTCTCAGC TCAAGCAGAC
    TGTAAACTTC TAGAGTGCAG AAATGTGACA ATGCAAAGCT TGGTGAAACC CTTCAGCATC
    TTTGGACAGA CTGCAATTTC CAGCAATGCA GTAGAAAAGC TGATTGATTG CTCCATCATC
    ATTGACTCAA TATTTGTAAA CTTTGGGCAA CATGTAGTTC ATTCTCTCAA CACTGCAGTA
    CAAGCTTGGC AACAGAACAA ATGCCCTGAG GTAGAGGAGT TGGTCTTCAG CCATTTTGTG
    ATCTGTAATG ACACACAGGA GACACTGCGG TTTGGCCAGG TGGATACTGA TGAAAATATC
    CTGCTGGCCA GTCTCTGCAG TCACCAGTAC AGCTGGCGCT CTCACAAATC CCCACAGCTG
    TTACACATCT GTATTGAAGG TTGGGGCAAC TGGCGTTGGT CAGAGCCCTT CAGTGTGGAC
    CATGCGGGGA CATTTATTAG AACTATTCAG TACAAGGGTC GAACTGCTTC ACTCATCATC
    AAGGCTCAGC AAGTCAGCGG CGTGCAAAAA CAGATTATCA TCTGTGGAAG ACAGATCATC
    TGTAGTTACT TGTCTCAAAG CATAGAATTA AAAGTGGTTC AGCATTACAT TGGTCAAGAT
    GGACAAGCTG TGGTTCGAGA ACACTTTGAC TGCCTCACAG CCAAACAGAA ATTGCCTTCG
    TACATACTAG AGAACAGCGA ACTGACTGAG TTGTGTGTGA GGGCCAAAGG AGATGAAGAC
    TGGTCAAGAG ACGTGTGCCT GGAATCCAAG GCTCCTGAGT ATAGTGTCGT CATTCAGGTG
    CCATCTTCAA ACAGTTCCAT TATTTATGTC TGGTGCACAG TTTTGACTTT AGAACCCAGC
    TCTCAAGTGC AGCAGCGAAT GATTGTGTTC AGCCCTCTTT TTATCATGAG GAGTCATCTT
    CCAGACCCCA TTATCATACA TTTGGAGAAA AGGAGTCTGG GATTGAGTGA AACACAAATT
    ATTCCAGGAA AAGGACAGGA AAAACCACTG CAAAACATAG AACCTGACCT TGTACATCAC
    CTAACATTTC AAGCAAGAGA AGAGGATGAC CCTTCACACT GTGCCGTTCC CATCTCAACA
    TCCCTCATTA AGCAGATAGC CACTAAGATA CACCCCGGAG GCACAGTTAC TCAGATCCTT
    GATGAGTTCT ACGGGCCAGA GAAGTTCCTT CAACCCACAT GGCCCTATAA TAAAAAGGAC
    TCTGACAGGA ATGAGCAACT GAGCCAGTGG GACAGCCCAA TGCGAGTGAA GCTGTCAGCC
    TGGAAGCCAT ATGTGAGGAC CTTATTGATA GAATTGCTAC CCTGGGCCCT GCTTATCAAT
    CAGTCCAAGT GGGACCTCTG GCTGTTTGAG GGAGAGAAGA TTGTTCTACA GGTTCCTGCT
    GGCAAAATTA TTATCCCTCC TAATTTTCAG GAAGCTTTTC AAATTGGAAT ATACTGGGCA
    AGTACAAACA CCGTGCACAA GTCAGTAGCT GTTAAGCTGG TCCATAACCT GACATCTCCA
    AAGTGGAAAG ACGGAGGTAA CGGCGAAGTT GTATCTTTGG ACGAGGAAGG GTTTGTTCAT
    GCTGAAATAA GACTCGGTGC TTTCCCAGGA CACCAGAAGT TGTGCCAGTT CTGCATTTCC
    TCCATGGTAC AGCAAGGCAT ACAGATTATT CAGATTGAAG ATAAGACCAC AATAATCAAT
    AACACACCAT ATCAGATATT TTATAAGCCA CAGTTAACTG TCACCAAACT CCACTCTGGA
    AAAGAGTATT TTCATGTTCC AGACAGCGCT ACTTTCAGCA TTTGCCCGGG TGGCGAGCAG
    CCTGCCGTGA GATCCAGCTC CCTTCCTTGC TGGGACCTAA TGCCCGACCT CGGCCAGTCC
    GCGCTGGACG CGTCCCTGCT GCAGAAGCAG GTCTTGCTGG CCTTTTCTCC CACCGCCGTT
    GCCGACAGCG CCCAGTGCTG GAGCCTGCCA GCCCTAGTCA GACAGGAGCT TCCCAGACAG
    AGCGTGGCAG TGCCCTTGGG GAACTACAGG GAAGATGGCT TCTGTACCAG GGCAATAGCA
    CTAACATATC AAGAACACCT TGGAGTGACT TATTTAACCC TCTCAGAAGA CCCTAGTCCT
    CGAATAATTT TCCACAACAG ATGTCCAGTG ACAATACTTA TAAAGGAAAA TACTAAAGAT
    ACTCCGAAGT TTGAGGTTTA TTGCAGAAAA ATTCCTCCTG AGTGCTCAAT TCATCATGAG
    CTATATCATC AGATTTCCAG TTATCCAGAC TGCAAGACCA GAGACCTCCT TCCTAGCCTC
    CTCTTGAGAG TGGAATTGCT GGAGGAAATG ACAACCGAGT GGAGTGACGC CATCGACATC
    AACAATCAGG GGACACAGGT TGTGTTTCTG ACTGGCCTTG GCTATGTGTA CGTGGACACC
    ATCCATCACT GTGGCACAGT CTTCATCAAC GTGGCCCCAG AAGGAAAAGC TGGGCCTGTC
    GTAACTAATA CCAACAGAAC TCTGGAGAAG ATCGTGACAT TTAAAATGTT CATCACTCAG
    TTGAGCCTGG CGGTGTTTGA TGACCTCACC CACCACAGAG CATCATCCGA GCTTTTGAGA
    CTCACACTGG ACCACGTTTT CCTCCAGATG GCCCCCGTGG CTGGTCACCT CCCTGGGGAA
    GAGATGGCCG CCGCCCCCTT TCACGTCTAC TCCGTGGAGG TCTGCTGCGG GGACCTGCAG
    CTGGACAACC AGCTGTACAA CAAATCCAAT TTCCACTTTG CTGTCTTGGT CTGCCAGGAG
    GAGAAAACAG AATCTCCTCA GTACTCCAAG ATGCAGAGTC TCCTCGTATC CAGTGAAGAT
    TTGGAAGAAT ACAAGAAAAA CTGCTTTATC AAACTCTGCC TCACGTTAAC CGAAGGCAAG
    GATGTCCTGT TCGATGTGAA CGAGTTCAGT TTTGAATTGA AACCTGCCCG GTTATATGTG
    GAAGACACGT TTGTCTACTA CATCAAAACT CTGTTTGAGA CCTACCTGCC CAGCAGCAGC
    CCGGGCGCTC AGCCCACGGC TCTCGCAGGC GGCAGACAGG TGCTGCCCAT GCAGGTGAGG
    CAGCACGCCC GGGCCTTGGT GCATCCCGTG AAGCTGCAGA AGCTGGTGAT ACAGCCTGTG
    AACCTCCTCG TCAGCATCCA TGCGTCCCTC AAGCTGTACA TCGCCTCTGA CCACACTCCG
    CTCTCCTTCT CAGTGTTTGA AAGAGGCCCC ATCTTCACCA CGGCCAGGCA GCTCGTCCAC
    GCCCTGGCCA TGCACTACGC CGCAGGGGCC CTCTTCAGAG CAGGCTGGGT GGTTGGATCC
    CTGGAAATCC TTGGCAGCCC GGCGAGTCTG GTGCGAAGCA TCGGGAACGG CATCGCTGAC
    TTCTTCAGGC TTCCGTACGA GGGGCTGACC CGAGGCCCGG GAGCCTTCGT GAGCGGGGTT
    TCCAGAGGGA CTACATCGTT TGTGAAGCAC ATTTCCAAAG GTACCCTCAC GTCCATCACC
    AACCTGGCCA CGAGCCTGGC CCGGAACATG GACCGGCTGT CGCTGGACGA GGAGCACTAC
    AACCGGCAGG AAGAGTGGCG GCGGCAGCTC CCGGAGAGCC TGGGCGAGGG GCTCCGGCAG
    GGCCTGTCCC GGCTGGGTAT CAGTCTGCTC GGTGCAATTG CTGGTATAGT TGATCAGCCA
    ATGCAGAACT TCCAGAAAAC ATCAGAGGCT CAAGCTTCAG CAGGACACAA GGCCAAGGGT
    GTTATCTCCG GCGTGGGGAA AGGAATCATG GGGGTGTTCA CGAAGCCCAT CGGAGGGGCT
    GCTGAGCTGG TGTCACAGAC TGGCTATGGT ATTTTACACG GAGCTGGACT TTCTCAGCTT
    CCCAAACAGC GCTATCAGCC AAGTGATCTG CACGCTGACC AGGCTCCAAA CAGCCACGTC
    AAATACGTCT GGAAGATGCT CCAGTCTCTG GGCAGACCGG AAGTACACAT GGCCCTGGAG
    GTTGTTCTGG TGAGGGGCTC GGGCCAGGAG CACGAGGGCT GCCTGCTGCT GACGTCAGAC
    GTGCTCTTCG TGGTGAGCGT CAGCGAGGAC ACCCAGCAGC AGGCCTTCCC AGTCACGGAG
    ATCGACTGCG CACAGGACCG CGAGCAGAGC AGCCTGCTCA TCGTGCAGCT CAAGCAGCCC
    AGAGTGGCCT GTGACGCAGA GGTGGATGGA GTTCGAGAGA GACTGTCAGA GCAACAGTAT
    AACAGACTTG TGGACTACAT CACAAAGACC TCTTGTCACC TGGGCCCCGG CTGCTCCTCC
    GTGCAGGCGT CATGCCCTGT GGTGGCTGCG GAACCGCTCC CCTCCACTGT GAAAACATAC
    CATTATATGG TTGATCCACA TTTTGCCCAA GTTTTCATTA GTAAATTTAC CATGGTGAAA
    AATAAAGCCC TGAGGAAAGG GTTCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120555.1
    CDS446..12355
    Translation

    Target ORF information:

    RefSeq Version XM_025264770.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAT GATCATGAAA GCTGTGGTTC TAATTCTACC
    AACCGTAGTA CTACTGAGAA CACAAAAACA TCAGCAAAAC CCCGAAGAAT TCAACAGGCC
    ACTCCCACAG ATCCTGATTT GCCACCAGGC TATGTACAGA GTCTGATCAG ACGAGTTGTA
    AATAATGTAA ATATTGTTAT AAATAATCTG ATACTAAAAT ATGTTGAAGA TGATATTGTC
    CTTTCAGTCA ACATCACATC CGCAGAATGC TATACAGTAG GTGAATTGTG GGATCGTGCA
    TTCATGGATA TTTCTGCAAC TGATCTGGTG TTGAGAAAGG TTATCAATTT TTCTGACTGT
    ACAGTTTGTC TTGATAAACG AAATGCCAGT GGTAAAATAG AATTCTACCA GGATCCTTTA
    TTGTACAAGT GTTCCTTCAG AACTCGTCTG CATTTTACTT ACGATAACCT AAATTCTAAG
    ATGCCATCAG TTATAAAAAT TCATACCTTA GTAGAGAGTT TGAAACTTTC TATCACTGAT
    CAGCAGTTGC CTATGTTTAT CCGTATAATG CAACTTGGAA TTGCTCTTTA CTATGGAGAA
    ATAGGAAATT TTAAAGATGG AGAAACCGAA GATTCTACTT GTCACAATAA AGATATGTTG
    GGAAATGTTA CAGGTGCTGA AGATGAAACA CGAATAGATA TGCAGTATCC TGCTCAGTAC
    AAAGGCCAAG AGCTGTATTC ACAGCAAGAG GACGACCAGC CACAGGGATG GGTATCATGG
    GCCTGGTCAT TTGTCCCTGC CATTGTGAAT TATGATGATG CCGAGGAAGA CTACCTTGGG
    AATGATCCTA CATCAACTAC ACATCAACAA AAAACACAGA CTTTGAAGGA TCCTATTGTT
    TCTATAGGAT TTTATTGTAC AAAGGCAACT GTGACTTTCA AACTTACTGA AATGCAAGCC
    GAGAGTAGTT ACTACAGCCC TCAAAAAGTA AAATCTAAAG AAGTATTATG TTGGGAACAA
    GAAGGAACTA CAGTTGAGGC CCTTATGATG GGAGAGCCTT TCTTTGATTG CCAGGTTGGT
    TTTGTAGGTT GCAGAGCCAT GTGCCTTAAA GGAATTATGG GTGTTAAAGA TTTTGAAGAG
    AATATGAATA GAAGTGAAAC CGAAGCCTGT TTCTTCCTTT GTGGTGAAAA TTTGAGTATA
    AAAGGTTTGA CATACCTCAC AAATTCATTG TTTGATTACC GAAGCCCAGA AAATAATGGT
    ACTCGTGCAG AATTTATCTT GGATGCAGCT ACTCATAAGG AGACATACAC TGAGATAGCT
    GGAATGCAAA GGTTCGGGGC ATTTTACATG GATTACCTGT ATACAATGGA GAACACCACT
    GGCAAAGGTT CTGCAAATCA ACAAGACTTT TCTTCAGGGA AGAGTGAAGA TTTGGGAACA
    GTTCAGGAAA AGTCTACCAA AAGCCTTGTT ATAGGTCCTC TTGATTTTCG CTTGGATAGC
    AGTGCAGTGC ACAGAATTTT AAAAATGGTT GTTTGTGCCT TGGAACATGA ATATGAACCA
    TATAGTAGAC TAAAACCAGA TATTAAAGAT GAAAGTGAAA CAATGCTGAA TCCTGAGGAA
    GTGGCATCTT TGGAGGAATA TATTCCTACT CGACATACAA GTGTTACTCT CCTCAAATGT
    ACCTTCACAA TTTTCATGGC TGAATTCAAT TTGCTGGATC ATTTGCTACC TGTTATTATG
    GGGGAAAAGA ACTCAAGTAA CTTCATGAAT ACTACAAACT TCCAGTCTCT CCGGCCATTG
    CCTTCTGTTC AGATATTGGT GGATAAAATT AATCTGGAAC ATTCTGTGCC GATGTATGCT
    GAACAGTTGG TGCATGTGGT CAGCAGTCTT ACTCAGCCTT CTGATAATCT GCTTCATTAT
    TGCTATGCAC ATTGCTACCT TAAGATATTT GGTTTCCAGG CAGGACTGAC GTCTTTGGAT
    TGTAGGGGAT CTTACTGCTT ACCTGTACCA ATTGTTCCCT CTTTCAGCAC TGCTCTTTAT
    GGTAAACTTC TGAAACTACC CACATGCTGG ACCAAAAGAT CTCAGATTGC TATAACTGAA
    GGCATTTTTG AGCTTCCAAA TCTTACAGTT CAAGCTACAA GAGCTCAGAC ACTTCTGCTT
    CAAGCAATAT ATCAAAGTTG GTCTCATAGT GGAAATGTCA GCTCTTTAGC AGTTAATGAA
    GCTTTGATGA ATGAAGTTTT CCAGACTGTA GGTGTGAAAT CTAAGAATCC CCTGCCAACT
    CTTGAGGGCT CAATCCAGAA TGTTGAATTG AAGTACTGCA GCACATCATT GGTCAAATGT
    GCCTCTGGGA CCGTGGGATC AATAAAAATT TGTGCCAAAG CCCCAGGTGA CAGTGGAAAA
    GAGAAGTTGA TTCCATTGCT TCAGGGTCCT TCTGACACTA AAGACCTTCA TAGCAGCAAG
    TGGCTCAATG AGAGCAGAAA GCCAGAATCT CTCTTAGCTC CAGATTTGAT AGCCTTCACA
    GTCCAGGTTC CACAGTGTAT GGACTACTGT CACAGTTCCG GTGCCGTACT CCTTTGTAAC
    GTCCAAGGAC TAGCAGTTAA TATTGACCCT ATCTTATACA CCTGGCTCAT TTATCAACCT
    CAGAAGCGAA GCAGTAAACA TATGCAGCAG CCTGTAATTG CTGTTCCTCT TGTTATGCCA
    ATTAGCAGAA GGAAAGAGGA TGAGGTGTCT GTTGGAAGTG CACCCTTGGC AAAGCAACAG
    TCATATCAGG CATCTGAGTA TGCCAGCAGC CCTGTAAAAA CAAAAACTGT AACAGAATCC
    AGACCATTGT CAGTTCCTGT GAAAGCTGTG CTGACGATAA CTGAAGGCTG TAGAAGTCCT
    GAAGAAAGAA TGAAAGAGTT TATTGGAATA GTATGGAATG CAGTTAAGAG TCTAACACTG
    CAGCTTGAAG TACAGTCTTG TTGTGTGTTT ATCCCAAATG ATAGTTTGCC TTCTCCTAGT
    ACAATTGTAT CTGGTGACAT TCCTGGAACA GTAAGAAGTT GGTACCATGG ACAAATGAGC
    ATGCCAGGAA CGCTTGTCCT CTGTTTGCCT CAAATAAAGA TTGTTAGTGC TGGCCACAAG
    TATATGGACC CTCTCCAGGA GATTCCGTTT GTCATCCCAC GACCCATCCT TGAAGAAGGT
    GATGCTTTTC CTTGGACAAT CAGCTTGCAT CATTTCAGCA TTTATACCCT TTTTGGAAAA
    CAAGTGACAC TTTGCCTAGT GGAACCCATG GGTTGTACCT CTACACTAGC TGTTACATCC
    CAAAAATTGC TTGCTACAGG ACCTGATATG AGGCATTCAT TTGTTGTCTG TCTGCATGTT
    GACCTAGAGT CACTTGAGAT AAAATGCTCA AACCCCCAGG TTCAGCTCTT ATATGAGTTA
    ACAGATATCA TGAATGAAGT CTGGAATAAG ATTCAGAAGA GAGGCAATCT CAGTTCTTCT
    TCAGCTTACC CGGAGTCCGT GGTGGGGCCT TTCCCTAGCT CTCCAGTTAG AAGCAGTATA
    GGCACAGCTC CTCCAGATAA TAGCACATGT AGCCCATCTG CTGACATTGG AACTACTACT
    GAGGGAGATT CTGTACAAGC TGTTGATGAT TCACCATTCT CAGATTCTGT TACCTTGGAA
    CAGACTACAA GTAACATTGG TGGATCCAGT GGTCGTGTTA GTCTGTGGAT GCAGTGGGTG
    CTCCCCAAAA TTACTGTAAA ACTCTTTGCT CCAGATCCTG ACAATAAAGG CATAGAACTT
    TGTATGGTCA GTGAACTAGA AGATCTCAGT GCTTCAATAG ATGTCCAGGA TGTGTACACT
    AAAGTGAAAT GTAAAATAGA AAGTTTCAAT ATTGATCACT ATAGAAGCAA CCTTGGGGAA
    GAGTGTTGGT CTCTGGGGCA ATGTGGAGGT GTCTTCCTTT CCTGTACTGA CAAGCTGAAC
    AGACGCACCT TGTTGGTTCG ACCCATCAGC AAGCAGGACC CTTTCAGTAA TTGCTCTGGC
    TTCTTTCCTT CTACAACTGC AAAGCTTCTA GATGGCTCTC ATCAGCAGCA TGGATTTCTG
    TCTTTGACAT ACACAAAAGC TGTAACAAAA AATGTTCGCC ACAAGTTAAC ATCAAGAAAT
    GAGCGAAGAA GTTTTCATAA GTTATCTGAA GGTTTAACAG ATGGCTCCCC TCATTTTCTT
    CATGAAATAC TTCTTTCAGC ACAAGCCTTT GATATTGTCC TTTGTTTTCC TTTACTTAAT
    GCCATTGCAA GTATATTTCA AGCAAAACTA CCAAGGACCC AAAAAGAGAA AAGAAAATCT
    CCTGGTCAGC CCATGAGGAC CCATACACTG ACTTCACGCA ATTTACCTTT GATTTATATC
    AACACAAGCG TAATCAGAAT TTTTGTTCCA AAAACAGAAG AAATGCAGCC AAGTATTGAA
    GTTAATCAGA CAGCAAAGGA AGATACCATG GTTCTGAAGA TTGGCTCTGT TGCTATGGCT
    CCCCAGGCTG ACAATCCCCT TGGCAGATCC GTCCTCAGGA AAGATATTTA CCAAAGAGCC
    TTGAATTTAG GAATTCTTCG AGACCCTGGG TCAGAGATTG AAGACCGACA ATACCAAATA
    GATCTGCAGT CGATCAATAT TGGTACTGCT CAGTGGGATC AACTGAAACC AGAAAAGGAA
    AATGGCACAG GAGGAGTGCT AACAGAGACT GAAAGAAATT CGCAGAATCC AGCCCTTGAG
    TGGAATATGG CCAGCAGCAT ACGGCGGCAT CAAGAAAGAA GAGCAATTTT GACTCCCATT
    TTAACAGATT TTTCTGTACG AATAACTGGA GCACCTGCCA TCATTTTCAC CAAAATAATT
    TCTCCGGAAA ATATGCACAC TGAGGAGATT TTAGTGTGTG GCCATTCCTT GGAAGTGAAT
    ATAACCACAA ACCTGGACTT CTTCCTAAGT GTGGCTCAAG TTCAACTCTT ACATCAGTTA
    ATAGTAGCAA ATATGACTGG ACTGGAACCA TCAAACAAAG CCACAGAGAT CGCTAAGCAA
    GAACAGAAAA AAATGGATAC ATATGATGGA ATCGTGGCTG ATAGCTCATC TCGGTACAGT
    GGTGCCCAGG ATAGTGGAAT CGGCAGTGAC AGCGTTAAAA TCAGAATAGT ACAGATAGAG
    CAGCACAGCG GTGCCAGTCA GCACCGCATC GCCCGTCCCT CACGCCAGTC GTCAATTGTA
    AAAAATCTAA ATTTTATTCC TTTTGACATA TTTATTACTG CAAGTAGAAT CTCACTAATG
    ACCTATTCCT GTACGGCCTT ACCCAAATCG AAATTGCAAG AACAAAAGGA TAATGAAAAA
    ACAGGCAAGA GTTCATTAAA CCTGCCAGAA GTCGATTCAG ATGTCGCAAA ACCCAGCCAG
    GCATGTATTT CCATGGTAAC AGCAGAAGAT CTCTTAAGCA GCAACATATC TTTTCCTTCA
    GGGAAAAAAA TAGGGGTCAT CTCTCTCGAA AGTCTGCATG CATCCACAAG GTCATCTGCT
    AGACAAGCCC TCGGCATAAC CATTGTTCGG CAGCCTGGAA GAAGAGGAAC CGGTGACTTG
    CGACTAGAAC CTTTCTTGTA CTTTATTGTA TCCCAGCCTT CTTTGCTTCT GAGTTGTCAC
    CACAGGAAGC AACGAGTGGA AATATCCATT TTTGATGCTG TGCTTAAAGG GGTTGCCTCT
    GATTACAAAT GTACAGATCC TGGGAAGACT TTGCCTGAAG CCCTTGATTA TTGCACAGTT
    TGGCTGCAGA CAGTACCTGG AGAAATAGAC AGCAAAAGTG GTATTCCACC TTCACTCTTA
    ACGCTACAAA TTAAAGATTT TCTTAACGGA CCAGCAGACA TCAACTTGGA TATATCAAAG
    CCTTTGAAAG CAAACCTGAG TTTTACCAAG TTGGATCAAA TAAACCATTT TTTGAAGAAG
    ATAAAAAATG TACACAGCAA AGCGTCCTCA GCACCGTCAG ACGCTGTGCT GAGTAAGGAT
    GCGCTTCCAG CCTCCAAATG TTACCGTGGG AAGTTATCCA AGCTTAAAGT TCATGGTGAT
    GGAGCACAAA AGATTCCATT TCAAGAAAAC ATATGGAGAA CGATGTCCTG CTTTCAAAAA
    ATTTCTGTTC ATACTACTCA GATTGTGGTC TCCATGGAAA CTGTCCCCCA TCCTAATAAA
    CCATGCCTGT TGGCATCTTT ATCCACCCTC AGTGGAAGCC TTAATGTCAA AGCAGCGCAG
    AAAGTACCTG GTGTGATTCT TGGGTCATCT TTTCTACTCA GCGTAAATGA TTTTCTTCTT
    AAAACGTGTC TCAAAGAAAG AAGTCGGATT CTAATAGGAC CGTGTTGTGC TACTGCCAAT
    TTGGAAGCTA AATGGTGTAA ACACAGTGGC GATCCAGGCC CAGAACAGTC CATACCAAAA
    ATATCCATTG ATGTAAGAGG AGGTCTGCTA CAGGTTTTCT GGGGTCAAGA ACATTTGAAT
    TGCTTAATTC TTCTACATGA ATTACTCAGT GGATACCTTA ACATGGAAGG AAATTTTGAA
    GCGAAAGTTT CTGAAGCAGT TCCACAAATG CCATCTCCTT TGGAAAAAAA TCAGATATTT
    AAAAGTGAAC AAAGTTCAGA TGACCTACGG ACAGGTCTAT TTCAGTATAT ACGGGATGCT
    GAACCTCTGA AAATGCCTGG TGTCTATGAA GTCTTGTTTT ACAATGAAAC CGAAGATAGC
    CCAGGGATGA TGCTGTGGAG ATACCCAGAA CCTAGAGTGC TCACCCTTGT ACGCATAACT
    CCTGTGCCCT TCAACACCAC CGAGGATCCA GACATTAGCA CAGCAGACCT TGGTGACATG
    CTGCAGATTC CTTGTAGTTT GGAATACTGG GATGAACTCC AGAAGGTTTT TGTTGCATTT
    CGAGAATTTA GTCTGTCTGA AAGCAAAGTT TGTGAACTGC AATTGCCAGA TATCAATCTT
    GTGAACGACC AGAAGAAACT GGTATCCTCG GATCTTTGGA GAATTGTCTT GAACAGCAGC
    CAAAATGGAG CTGATGACCA AAGCTCTGCA AGTGAATCTG GTTCTCAAAG CACTTGTGAC
    CAGCTTGTAA CCCCAACAGC TCTGGCTGCC TGTACCAGAG TTGACTCCTG CTTTACCCCG
    TGGTTTGTCC CATCTCTTTG CATTTCCTTC CAGTTTGCTC ACCTGGAGTT CCATCTTTGT
    CATCACCTTG ACCAGCTTGG CACAGCTCCA CCAAAATACC TACAGCCATT TATTTCTGAT
    AAAAATGTGC CATCTGAACT AGAATACATG GTTATTTCCT TCAAAGAACC ACACATGTAT
    CTTCGACAGT GGAACAATAG TTCTGTCTGT CAGGAGATCC AGTTCTCAGC TCAAGCAGAC
    TGTAAACTTC TAGAGTGCAG AAATGTGACA ATGCAAAGCT TGGTGAAACC CTTCAGCATC
    TTTGGACAGA CTGCAATTTC CAGCAATGCA GTAGAAAAGC TGATTGATTG CTCCATCATC
    ATTGACTCAA TATTTGTAAA CTTTGGGCAA CATGTAGTTC ATTCTCTCAA CACTGCAGTA
    CAAGCTTGGC AACAGAACAA ATGCCCTGAG GTAGAGGAGT TGGTCTTCAG CCATTTTGTG
    ATCTGTAATG ACACACAGGA GACACTGCGG TTTGGCCAGG TGGATACTGA TGAAAATATC
    CTGCTGGCCA GTCTCTGCAG TCACCAGTAC AGCTGGCGCT CTCACAAATC CCCACAGCTG
    TTACACATCT GTATTGAAGG TTGGGGCAAC TGGCGTTGGT CAGAGCCCTT CAGTGTGGAC
    CATGCGGGGA CATTTATTAG AACTATTCAG TACAAGGGTC GAACTGCTTC ACTCATCATC
    AAGGCTCAGC AAGTCAGCGG CGTGCAAAAA CAGATTATCA TCTGTGGAAG ACAGATCATC
    TGTAGTTACT TGTCTCAAAG CATAGAATTA AAAGTGGTTC AGCATTACAT TGGTCAAGAT
    GGACAAGCTG TGGTTCGAGA ACACTTTGAC TGCCTCACAG CCAAACAGAA ATTGCCTTCG
    TACATACTAG AGAACAGCGA ACTGACTGAG TTGTGTGTGA GGGCCAAAGG AGATGAAGAC
    TGGTCAAGAG ACGTGTGCCT GGAATCCAAG GCTCCTGAGT ATAGTGTCGT CATTCAGGTG
    CCATCTTCAA ACAGTTCCAT TATTTATGTC TGGTGCACAG TTTTGACTTT AGAACCCAGC
    TCTCAAGTGC AGCAGCGAAT GATTGTGTTC AGCCCTCTTT TTATCATGAG GAGTCATCTT
    CCAGACCCCA TTATCATACA TTTGGAGAAA AGGAGTCTGG GATTGAGTGA AACACAAATT
    ATTCCAGGAA AAGGACAGGA AAAACCACTG CAAAACATAG AACCTGACCT TGTACATCAC
    CTAACATTTC AAGCAAGAGA AGAGGATGAC CCTTCACACT GTGCCGTTCC CATCTCAACA
    TCCCTCATTA AGCAGATAGC CACTAAGATA CACCCCGGAG GCACAGTTAC TCAGATCCTT
    GATGAGTTCT ACGGGCCAGA GAAGTTCCTT CAACCCACAT GGCCCTATAA TAAAAAGGAC
    TCTGACAGGA ATGAGCAACT GAGCCAGTGG GACAGCCCAA TGCGAGTGAA GCTGTCAGCC
    TGGAAGCCAT ATGTGAGGAC CTTATTGATA GAATTGCTAC CCTGGGCCCT GCTTATCAAT
    CAGTCCAAGT GGGACCTCTG GCTGTTTGAG GGAGAGAAGA TTGTTCTACA GGTTCCTGCT
    GGCAAAATTA TTATCCCTCC TAATTTTCAG GAAGCTTTTC AAATTGGAAT ATACTGGGCA
    AGTACAAACA CCGTGCACAA GTCAGTAGCT GTTAAGCTGG TCCATAACCT GACATCTCCA
    AAGTGGAAAG ACGGAGGTAA CGGCGAAGTT GTATCTTTGG ACGAGGAAGG GTTTGTTCAT
    GCTGAAATAA GACTCGGTGC TTTCCCAGGA CACCAGAAGT TGTGCCAGTT CTGCATTTCC
    TCCATGGTAC AGCAAGGCAT ACAGATTATT CAGATTGAAG ATAAGACCAC AATAATCAAT
    AACACACCAT ATCAGATATT TTATAAGCCA CAGTTAACTG TCACCAAACT CCACTCTGGA
    AAAGAGTATT TTCATGTTCC AGACAGCGCT ACTTTCAGCA TTTGCCCGGG TGGCGAGCAG
    CCTGCCGTGA GATCCAGCTC CCTTCCTTGC TGGGACCTAA TGCCCGACCT CGGCCAGTCC
    GCGCTGGACG CGTCCCTGCT GCAGAAGCAG GTCTTGCTGG CCTTTTCTCC CACCGCCGTT
    GCCGACAGCG CCCAGTGCTG GAGCCTGCCA GCCCTAGTCA GACAGGAGCT TCCCAGACAG
    AGCGTGGCAG TGCCCTTGGG GAACTACAGG GAAGATGGCT TCTGTACCAG GGCAATAGCA
    CTAACATATC AAGAACACCT TGGAGTGACT TATTTAACCC TCTCAGAAGA CCCTAGTCCT
    CGAATAATTT TCCACAACAG ATGTCCAGTG ACAATACTTA TAAAGGAAAA TACTAAAGAT
    ACTCCGAAGT TTGAGGTTTA TTGCAGAAAA ATTCCTCCTG AGTGCTCAAT TCATCATGAG
    CTATATCATC AGATTTCCAG TTATCCAGAC TGCAAGACCA GAGACCTCCT TCCTAGCCTC
    CTCTTGAGAG TGGAATTGCT GGAGGAAATG ACAACCGAGT GGAGTGACGC CATCGACATC
    AACAATCAGG GGACACAGGT TGTGTTTCTG ACTGGCCTTG GCTATGTGTA CGTGGACACC
    ATCCATCACT GTGGCACAGT CTTCATCAAC GTGGCCCCAG AAGGAAAAGC TGGGCCTGTC
    GTAACTAATA CCAACAGAAC TCTGGAGAAG ATCGTGACAT TTAAAATGTT CATCACTCAG
    TTGAGCCTGG CGGTGTTTGA TGACCTCACC CACCACAGAG CATCATCCGA GCTTTTGAGA
    CTCACACTGG ACCACGTTTT CCTCCAGATG GCCCCCGTGG CTGGTCACCT CCCTGGGGAA
    GAGATGGCCG CCGCCCCCTT TCACGTCTAC TCCGTGGAGG TCTGCTGCGG GGACCTGCAG
    CTGGACAACC AGCTGTACAA CAAATCCAAT TTCCACTTTG CTGTCTTGGT CTGCCAGGAG
    GAGAAAACAG AATCTCCTCA GTACTCCAAG ATGCAGAGTC TCCTCGTATC CAGTGAAGAT
    TTGGAAGAAT ACAAGAAAAA CTGCTTTATC AAACTCTGCC TCACGTTAAC CGAAGGCAAG
    GATGTCCTGT TCGATGTGAA CGAGTTCAGT TTTGAATTGA AACCTGCCCG GTTATATGTG
    GAAGACACGT TTGTCTACTA CATCAAAACT CTGTTTGAGA CCTACCTGCC CAGCAGCAGC
    CCGGGCGCTC AGCCCACGGC TCTCGCAGGC GGCAGACAGG TGCTGCCCAT GCAGGTGAGG
    CAGCACGCCC GGGCCTTGGT GCATCCCGTG AAGCTGCAGA AGCTGGTGAT ACAGCCTGTG
    AACCTCCTCG TCAGCATCCA TGCGTCCCTC AAGCTGTACA TCGCCTCTGA CCACACTCCG
    CTCTCCTTCT CAGTGTTTGA AAGAGGCCCC ATCTTCACCA CGGCCAGGCA GCTCGTCCAC
    GCCCTGGCCA TGCACTACGC CGCAGGGGCC CTCTTCAGAG CAGGCTGGGT GGTTGGATCC
    CTGGAAATCC TTGGCAGCCC GGCGAGTCTG GTGCGAAGCA TCGGGAACGG CATCGCTGAC
    TTCTTCAGGC TTCCGTACGA GGGGCTGACC CGAGGCCCGG GAGCCTTCGT GAGCGGGGTT
    TCCAGAGGGA CTACATCGTT TGTGAAGCAC ATTTCCAAAG GTACCCTCAC GTCCATCACC
    AACCTGGCCA CGAGCCTGGC CCGGAACATG GACCGGCTGT CGCTGGACGA GGAGCACTAC
    AACCGGCAGG AAGAGTGGCG GCGGCAGCTC CCGGAGAGCC TGGGCGAGGG GCTCCGGCAG
    GGCCTGTCCC GGCTGGGTAT CAGTCTGCTC GGTGCAATTG CTGGTATAGT TGATCAGCCA
    ATGCAGAACT TCCAGAAAAC ATCAGAGGCT CAAGCTTCAG CAGGACACAA GGCCAAGGGT
    GTTATCTCCG GCGTGGGGAA AGGAATCATG GGGGTGTTCA CGAAGCCCAT CGGAGGGGCT
    GCTGAGCTGG TGTCACAGAC TGGCTATGGT ATTTTACACG GAGCTGGACT TTCTCAGCTT
    CCCAAACAGC GCTATCAGCC AAGTGATCTG CACGCTGACC AGGCTCCAAA CAGCCACGTC
    AAATACGTCT GGAAGATGCT CCAGTCTCTG GGCAGACCGG AAGTACACAT GGCCCTGGAG
    GTTGTTCTGG TGAGGGGCTC GGGCCAGGAG CACGAGGGCT GCCTGCTGCT GACGTCAGAC
    GTGCTCTTCG TGGTGAGCGT CAGCGAGGAC ACCCAGCAGC AGGCCTTCCC AGTCACGGAG
    ATCGACTGCG CACAGGACCG CGAGCAGAGC AGCCTGCTCA TCGTGCAGCT CAAGCAGCCC
    AGAGTGGCCT GTGACGCAGA GGTGGATGGA GTTCGAGAGA GACTGTCAGA GCAACAGTAT
    AACAGACTTG TGGACTACAT CACAAAGACC TCTTGTCACC TGGGCCCCGG CTGCTCCTCC
    GTGCAGGCGT CATGCCCTGT GGTGGCTGCG GAACCGCTCC CCTCCACTGT GAAAACATAC
    CATTATATGG TTGATCCACA TTTTGCCCAA GTTTTCATTA GTAAATTTAC CATGGTGAAA
    AATAAAGCCC TGAGGAAAGG GTTCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49002
    Clone ID Related Accession (Same CDS sequence) XM_025264771.1
    Accession Version XM_025264771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GATATTAAAG ATGAAAGTGA AACAATGCTG
    AATCCTGAGG AAGTGGCATC TTTGGAGGAA TATATTCCTA CTCGACATAC AAGTGTTACT
    CTCCTCAAAT GTACCTTCAC AATTTTCATG GCTGAATTCA ATTTGCTGGA TCATTTGCTA
    CCTGTTATTA TGGGGGAAAA GAACTCAAGT AACTTCATGA ATACTACAAA CTTCCAGTCT
    CTCCGGCCAT TGCCTTCTGT TCAGATATTG GTGGATAAAA TTAATCTGGA ACATTCTGTG
    CCGATGTATG CTGAACAGTT GGTGCATGTG GTCAGCAGTC TTACTCAGCC TTCTGATAAT
    CTGCTTCATT ATTGCTATGC ACATTGCTAC CTTAAGATAT TTGGTTTCCA GGCAGGACTG
    ACGTCTTTGG ATTGTAGGGG ATCTTACTGC TTACCTGTAC CAATTGTTCC CTCTTTCAGC
    ACTGCTCTTT ATGGTAAACT TCTGAAACTA CCCACATGCT GGACCAAAAG ATCTCAGATT
    GCTATAACTG AAGGCATTTT TGAGCTTCCA AATCTTACAG TTCAAGCTAC AAGAGCTCAG
    ACACTTCTGC TTCAAGCAAT ATATCAAAGT TGGTCTCATA GTGGAAATGT CAGCTCTTTA
    GCAGTTAATG AAGCTTTGAT GAATGAAGTT TTCCAGACTG TAGGTGTGAA ATCTAAGAAT
    CCCCTGCCAA CTCTTGAGGG CTCAATCCAG AATGTTGAAT TGAAGTACTG CAGCACATCA
    TTGGTCAAAT GTGCCTCTGG GACCGTGGGA TCAATAAAAA TTTGTGCCAA AGCCCCAGGT
    GACAGTGGAA AAGAGAAGTT GATTCCATTG CTTCAGGGTC CTTCTGACAC TAAAGACCTT
    CATAGCAGCA AGTGGCTCAA TGAGAGCAGA AAGCCAGAAT CTCTCTTAGC TCCAGATTTG
    ATAGCCTTCA CAGTCCAGGT TCCACAGTGT ATGGACTACT GTCACAGTTC CGGTGCCGTA
    CTCCTTTGTA ACGTCCAAGG ACTAGCAGTT AATATTGACC CTATCTTATA CACCTGGCTC
    ATTTATCAAC CTCAGAAGCG AAGCAGTAAA CATATGCAGC AGCCTGTAAT TGCTGTTCCT
    CTTGTTATGC CAATTAGCAG AAGGAAAGAG GATGAGGTGT CTGTTGGAAG TGCACCCTTG
    GCAAAGCAAC AGTCATATCA GGCATCTGAG TATGCCAGCA GCCCTGTAAA AACAAAAACT
    GTAACAGAAT CCAGACCATT GTCAGTTCCT GTGAAAGCTG TGCTGACGAT AACTGAAGGC
    TGTAGAAGTC CTGAAGAAAG AATGAAAGAG TTTATTGGAA TAGTATGGAA TGCAGTTAAG
    AGTCTAACAC TGCAGCTTGA AGTACAGTCT TGTTGTGTGT TTATCCCAAA TGATAGTTTG
    CCTTCTCCTA GTACAATTGT ATCTGGTGAC ATTCCTGGAA CAGTAAGAAG TTGGTACCAT
    GGACAAATGA GCATGCCAGG AACGCTTGTC CTCTGTTTGC CTCAAATAAA GATTGTTAGT
    GCTGGCCACA AGTATATGGA CCCTCTCCAG GAGATTCCGT TTGTCATCCC ACGACCCATC
    CTTGAAGAAG GTGATGCTTT TCCTTGGACA ATCAGCTTGC ATCATTTCAG CATTTATACC
    CTTTTTGGAA AACAAGTGAC ACTTTGCCTA GTGGAACCCA TGGGTTGTAC CTCTACACTA
    GCTGTTACAT CCCAAAAATT GCTTGCTACA GGACCTGATA TGAGGCATTC ATTTGTTGTC
    TGTCTGCATG TTGACCTAGA GTCACTTGAG ATAAAATGCT CAAACCCCCA GGTTCAGCTC
    TTATATGAGT TAACAGATAT CATGAATGAA GTCTGGAATA AGATTCAGAA GAGAGGCAAT
    CTCAGTTCTT CTTCAGCTTA CCCGGAGTCC GTGGTGGGGC CTTTCCCTAG CTCTCCAGTT
    AGAAGCAGTA TAGGCACAGC TCCTCCAGAT AATAGCACAT GTAGCCCATC TGCTGACATT
    GGAACTACTA CTGAGGGAGA TTCTGTACAA GCTGTTGATG ATTCACCATT CTCAGATTCT
    GTTACCTTGG AACAGACTAC AAGTAACATT GGTGGATCCA GTGGTCGTGT TAGTCTGTGG
    ATGCAGTGGG TGCTCCCCAA AATTACTGTA AAACTCTTTG CTCCAGATCC TGACAATAAA
    GGCATAGAAC TTTGTATGGT CAGTGAACTA GAAGATCTCA GTGCTTCAAT AGATGTCCAG
    GATGTGTACA CTAAAGTGAA ATGTAAAATA GAAAGTTTCA ATATTGATCA CTATAGAAGC
    AACCTTGGGG AAGAGTGTTG GTCTCTGGGG CAATGTGGAG GTGTCTTCCT TTCCTGTACT
    GACAAGCTGA ACAGACGCAC CTTGTTGGTT CGACCCATCA GCAAGCAGGA CCCTTTCAGT
    AATTGCTCTG GCTTCTTTCC TTCTACAACT GCAAAGCTTC TAGATGGCTC TCATCAGCAG
    CATGGATTTC TGTCTTTGAC ATACACAAAA GCTGTAACAA AAAATGTTCG CCACAAGTTA
    ACATCAAGAA ATGAGCGAAG AAGTTTTCAT AAGTTATCTG AAGGTTTAAC AGATGGCTCC
    CCTCATTTTC TTCATGAAAT ACTTCTTTCA GCACAAGCCT TTGATATTGT CCTTTGTTTT
    CCTTTACTTA ATGCCATTGC AAGTATATTT CAAGCAAAAC TACCAAGGAC CCAAAAAGAG
    AAAAGAAAAT CTCCTGGTCA GCCCATGAGG ACCCATACAC TGACTTCACG CAATTTACCT
    TTGATTTATA TCAACACAAG CGTAATCAGA ATTTTTGTTC CAAAAACAGA AGAAATGCAG
    CCAAGTATTG AAGTTAATCA GACAGCAAAG GAAGATACCA TGGTTCTGAA GATTGGCTCT
    GTTGCTATGG CTCCCCAGGC TGACAATCCC CTTGGCAGAT CCGTCCTCAG GAAAGATATT
    TACCAAAGAG CCTTGAATTT AGGAATTCTT CGAGACCCTG GGTCAGAGAT TGAAGACCGA
    CAATACCAAA TAGATCTGCA GTCGATCAAT ATTGGTACTG CTCAGTGGGA TCAACTGAAA
    CCAGAAAAGG AAAATGGCAC AGGAGGAGTG CTAACAGAGA CTGAAAGAAA TTCGCAGAAT
    CCAGCCCTTG AGTGGAATAT GGCCAGCAGC ATACGGCGGC ATCAAGAAAG AAGAGCAATT
    TTGACTCCCA TTTTAACAGA TTTTTCTGTA CGAATAACTG GAGCACCTGC CATCATTTTC
    ACCAAAATAA TTTCTCCGGA AAATATGCAC ACTGAGGAGA TTTTAGTGTG TGGCCATTCC
    TTGGAAGTGA ATATAACCAC AAACCTGGAC TTCTTCCTAA GTGTGGCTCA AGTTCAACTC
    TTACATCAGT TAATAGTAGC AAATATGACT GGACTGGAAC CATCAAACAA AGCCACAGAG
    ATCGCTAAGC AAGAACAGAA AAAAATGGAT ACATATGATG GAATCGTGGC TGATAGCTCA
    TCTCGGTACA GTGGTGCCCA GGATAGTGGA ATCGGCAGTG ACAGCGTTAA AATCAGAATA
    GTACAGATAG AGCAGCACAG CGGTGCCAGT CAGCACCGCA TCGCCCGTCC CTCACGCCAG
    TCGTCAATTG TAAAAAATCT AAATTTTATT CCTTTTGACA TATTTATTAC TGCAAGTAGA
    ATCTCACTAA TGACCTATTC CTGTACGGCC TTACCCAAAT CGAAATTGCA AGAACAAAAG
    GATAATGAAA AAACAGGCAA GAGTTCATTA AACCTGCCAG AAGTCGATTC AGATGTCGCA
    AAACCCAGCC AGGCATGTAT TTCCATGGTA ACAGCAGAAG ATCTCTTAAG CAGCAACATA
    TCTTTTCCTT CAGGGAAAAA AATAGGGGTC ATCTCTCTCG AAAGTCTGCA TGCATCCACA
    AGGTCATCTG CTAGACAAGC CCTCGGCATA ACCATTGTTC GGCAGCCTGG AAGAAGAGGA
    ACCGGTGACT TGCGACTAGA ACCTTTCTTG TACTTTATTG TATCCCAGCC TTCTTTGCTT
    CTGAGTTGTC ACCACAGGAA GCAACGAGTG GAAATATCCA TTTTTGATGC TGTGCTTAAA
    GGGGTTGCCT CTGATTACAA ATGTACAGAT CCTGGGAAGA CTTTGCCTGA AGCCCTTGAT
    TATTGCACAG TTTGGCTGCA GACAGTACCT GGAGAAATAG ACAGCAAAAG TGGTATTCCA
    CCTTCACTCT TAACGCTACA AATTAAAGAT TTTCTTAACG GACCAGCAGA CATCAACTTG
    GATATATCAA AGCCTTTGAA AGCAAACCTG AGTTTTACCA AGTTGGATCA AATAAACCAT
    TTTTTGAAGA AGATAAAAAA TGTACACAGC AAAGCGTCCT CAGCACCGTC AGACGCTGTG
    CTGAGTAAGG ATGCGCTTCC AGCCTCCAAA TGTTACCGTG GGAAGTTATC CAAGCTTAAA
    GTTCATGGTG ATGGAGCACA AAAGATTCCA TTTCAAGAAA ACATATGGAG AACGATGTCC
    TGCTTTCAAA AAATTTCTGT TCATACTACT CAGATTGTGG TCTCCATGGA AACTGTCCCC
    CATCCTAATA AACCATGCCT GTTGGCATCT TTATCCACCC TCAGTGGAAG CCTTAATGTC
    AAAGCAGCGC AGAAAGTACC TGGTGTGATT CTTGGGTCAT CTTTTCTACT CAGCGTAAAT
    GATTTTCTTC TTAAAACGTG TCTCAAAGAA AGAAGTCGGA TTCTAATAGG ACCGTGTTGT
    GCTACTGCCA ATTTGGAAGC TAAATGGTGT AAACACAGTG GCGATCCAGG CCCAGAACAG
    TCCATACCAA AAATATCCAT TGATGTAAGA GGAGGTCTGC TACAGGTTTT CTGGGGTCAA
    GAACATTTGA ATTGCTTAAT TCTTCTACAT GAATTACTCA GTGGATACCT TAACATGGAA
    GGAAATTTTG AAGCGAAAGT TTCTGAAGCA GTTCCACAAA TGCCATCTCC TTTGGAAAAA
    AATCAGATAT TTAAAAGTGA ACAAAGTTCA GATGACCTAC GGACAGGTCT ATTTCAGTAT
    ATACGGGATG CTGAACCTCT GAAAATGCCT GGTGTCTATG AAGTCTTGTT TTACAATGAA
    ACCGAAGATA GCCCAGGGAT GATGCTGTGG AGATACCCAG AACCTAGAGT GCTCACCCTT
    GTACGCATAA CTCCTGTGCC CTTCAACACC ACCGAGGATC CAGACATTAG CACAGCAGAC
    CTTGGTGACA TGCTGCAGAT TCCTTGTAGT TTGGAATACT GGGATGAACT CCAGAAGGTT
    TTTGTTGCAT TTCGAGAATT TAGTCTGTCT GAAAGCAAAG TTTGTGAACT GCAATTGCCA
    GATATCAATC TTGTGAACGA CCAGAAGAAA CTGGTATCCT CGGATCTTTG GAGAATTGTC
    TTGAACAGCA GCCAAAATGG AGCTGATGAC CAAAGCTCTG CAAGTGAATC TGGTTCTCAA
    AGCACTTGTG ACCAGCTTGT AACCCCAACA GCTCTGGCTG CCTGTACCAG AGTTGACTCC
    TGCTTTACCC CGTGGTTTGT CCCATCTCTT TGCATTTCCT TCCAGTTTGC TCACCTGGAG
    TTCCATCTTT GTCATCACCT TGACCAGCTT GGCACAGCTC CACCAAAATA CCTACAGCCA
    TTTATTTCTG ATAAAAATGT GCCATCTGAA CTAGAATACA TGGTTATTTC CTTCAAAGAA
    CCACACATGT ATCTTCGACA GTGGAACAAT AGTTCTGTCT GTCAGGAGAT CCAGTTCTCA
    GCTCAAGCAG ACTGTAAACT TCTAGAGTGC AGAAATGTGA CAATGCAAAG CTTGGTGAAA
    CCCTTCAGCA TCTTTGGACA GACTGCAATT TCCAGCAATG CAGTAGAAAA GCTGATTGAT
    TGCTCCATCA TCATTGACTC AATATTTGTA AACTTTGGGC AACATGTAGT TCATTCTCTC
    AACACTGCAG TACAAGCTTG GCAACAGAAC AAATGCCCTG AGGTAGAGGA GTTGGTCTTC
    AGCCATTTTG TGATCTGTAA TGACACACAG GAGACACTGC GGTTTGGCCA GGTGGATACT
    GATGAAAATA TCCTGCTGGC CAGTCTCTGC AGTCACCAGT ACAGCTGGCG CTCTCACAAA
    TCCCCACAGC TGTTACACAT CTGTATTGAA GGTTGGGGCA ACTGGCGTTG GTCAGAGCCC
    TTCAGTGTGG ACCATGCGGG GACATTTATT AGAACTATTC AGTACAAGGG TCGAACTGCT
    TCACTCATCA TCAAGGCTCA GCAAGTCAGC GGCGTGCAAA AACAGATTAT CATCTGTGGA
    AGACAGATCA TCTGTAGTTA CTTGTCTCAA AGCATAGAAT TAAAAGTGGT TCAGCATTAC
    ATTGGTCAAG ATGGACAAGC TGTGGTTCGA GAACACTTTG ACTGCCTCAC AGCCAAACAG
    AAATTGCCTT CGTACATACT AGAGAACAGC GAACTGACTG AGTTGTGTGT GAGGGCCAAA
    GGAGATGAAG ACTGGTCAAG AGACGTGTGC CTGGAATCCA AGGCTCCTGA GTATAGTGTC
    GTCATTCAGG TGCCATCTTC AAACAGTTCC ATTATTTATG TCTGGTGCAC AGTTTTGACT
    TTAGAACCCA GCTCTCAAGT GCAGCAGCGA ATGATTGTGT TCAGCCCTCT TTTTATCATG
    AGGAGTCATC TTCCAGACCC CATTATCATA CATTTGGAGA AAAGGAGTCT GGGATTGAGT
    GAAACACAAA TTATTCCAGG AAAAGGACAG GAAAAACCAC TGCAAAACAT AGAACCTGAC
    CTTGTACATC ACCTAACATT TCAAGCAAGA GAAGAGGATG ACCCTTCACA CTGTGCCGTT
    CCCATCTCAA CATCCCTCAT TAAGCAGATA GCCACTAAGA TACACCCCGG AGGCACAGTT
    ACTCAGATCC TTGATGAGTT CTACGGGCCA GAGAAGTTCC TTCAACCCAC ATGGCCCTAT
    AATAAAAAGG ACTCTGACAG GAATGAGCAA CTGAGCCAGT GGGACAGCCC AATGCGAGTG
    AAGCTGTCAG CCTGGAAGCC ATATGTGAGG ACCTTATTGA TAGAATTGCT ACCCTGGGCC
    CTGCTTATCA ATCAGTCCAA GTGGGACCTC TGGCTGTTTG AGGGAGAGAA GATTGTTCTA
    CAGGTTCCTG CTGGCAAAAT TATTATCCCT CCTAATTTTC AGGAAGCTTT TCAAATTGGA
    ATATACTGGG CAAGTACAAA CACCGTGCAC AAGTCAGTAG CTGTTAAGCT GGTCCATAAC
    CTGACATCTC CAAAGTGGAA AGACGGAGGT AACGGCGAAG TTGTATCTTT GGACGAGGAA
    GGGTTTGTTC ATGCTGAAAT AAGACTCGGT GCTTTCCCAG GACACCAGAA GTTGTGCCAG
    TTCTGCATTT CCTCCATGGT ACAGCAAGGC ATACAGATTA TTCAGATTGA AGATAAGACC
    ACAATAATCA ATAACACACC ATATCAGATA TTTTATAAGC CACAGTTAAC TGTCACCAAA
    CTCCACTCTG GAAAAGAGTA TTTTCATGTT CCAGACAGCG CTACTTTCAG CATTTGCCCG
    GGTGGCGAGC AGCCTGCCGT GAGATCCAGC TCCCTTCCTT GCTGGGACCT AATGCCCGAC
    CTCGGCCAGT CCGCGCTGGA CGCGTCCCTG CTGCAGAAGC AGGTCTTGCT GGCCTTTTCT
    CCCACCGCCG TTGCCGACAG CGCCCAGTGC TGGAGCCTGC CAGCCCTAGT CAGACAGGAG
    CTTCCCAGAC AGAGCGTGGC AGTGCCCTTG GGGAACTACA GGGAAGATGG CTTCTGTACC
    AGGGCAATAG CACTAACATA TCAAGAACAC CTTGGAGTGA CTTATTTAAC CCTCTCAGAA
    GACCCTAGTC CTCGAATAAT TTTCCACAAC AGATGTCCAG TGACAATACT TATAAAGGAA
    AATACTAAAG ATACTCCGAA GTTTGAGGTT TATTGCAGAA AAATTCCTCC TGAGTGCTCA
    ATTCATCATG AGCTATATCA TCAGATTTCC AGTTATCCAG ACTGCAAGAC CAGAGACCTC
    CTTCCTAGCC TCCTCTTGAG AGTGGAATTG CTGGAGGAAA TGACAACCGA GTGGAGTGAC
    GCCATCGACA TCAACAATCA GGGGACACAG GTTGTGTTTC TGACTGGCCT TGGCTATGTG
    TACGTGGACA CCATCCATCA CTGTGGCACA GTCTTCATCA ACGTGGCCCC AGAAGGAAAA
    GCTGGGCCTG TCGTAACTAA TACCAACAGA ACTCTGGAGA AGATCGTGAC ATTTAAAATG
    TTCATCACTC AGTTGAGCCT GGCGGTGTTT GATGACCTCA CCCACCACAG AGCATCATCC
    GAGCTTTTGA GACTCACACT GGACCACGTT TTCCTCCAGA TGGCCCCCGT GGCTGGTCAC
    CTCCCTGGGG AAGAGATGGC CGCCGCCCCC TTTCACGTCT ACTCCGTGGA GGTCTGCTGC
    GGGGACCTGC AGCTGGACAA CCAGCTGTAC AACAAATCCA ATTTCCACTT TGCTGTCTTG
    GTCTGCCAGG AGGAGAAAAC AGAATCTCCT CAGTACTCCA AGATGCAGAG TCTCCTCGTA
    TCCAGTGAAG ATTTGGAAGA ATACAAGAAA AACTGCTTTA TCAAACTCTG CCTCACGTTA
    ACCGAAGGCA AGGATGTCCT GTTCGATGTG AACGAGTTCA GTTTTGAATT GAAACCTGCC
    CGGTTATATG TGGAAGACAC GTTTGTCTAC TACATCAAAA CTCTGTTTGA GACCTACCTG
    CCCAGCAGCA GCCCGGGCGC TCAGCCCACG GCTCTCGCAG GCGGCAGACA GGTGCTGCCC
    ATGCAGGTGA GGCAGCACGC CCGGGCCTTG GTGCATCCCG TGAAGCTGCA GAAGCTGGTG
    ATACAGCCTG TGAACCTCCT CGTCAGCATC CATGCGTCCC TCAAGCTGTA CATCGCCTCT
    GACCACACTC CGCTCTCCTT CTCAGTGTTT GAAAGAGGCC CCATCTTCAC CACGGCCAGG
    CAGCTCGTCC ACGCCCTGGC CATGCACTAC GCCGCAGGGG CCCTCTTCAG AGCAGGCTGG
    GTGGTTGGAT CCCTGGAAAT CCTTGGCAGC CCGGCGAGTC TGGTGCGAAG CATCGGGAAC
    GGCATCGCTG ACTTCTTCAG GCTTCCGTAC GAGGGGCTGA CCCGAGGCCC GGGAGCCTTC
    GTGAGCGGGG TTTCCAGAGG GACTACATCG TTTGTGAAGC ACATTTCCAA AGGTACCCTC
    ACGTCCATCA CCAACCTGGC CACGAGCCTG GCCCGGAACA TGGACCGGCT GTCGCTGGAC
    GAGGAGCACT ACAACCGGCA GGAAGAGTGG CGGCGGCAGC TCCCGGAGAG CCTGGGCGAG
    GGGCTCCGGC AGGGCCTGTC CCGGCTGGGT ATCAGTCTGC TCGGTGCAAT TGCTGGTATA
    GTTGATCAGC CAATGCAGAA CTTCCAGAAA ACATCAGAGG CTCAAGCTTC AGCAGGACAC
    AAGGCCAAGG GTGTTATCTC CGGCGTGGGG AAAGGAATCA TGGGGGTGTT CACGAAGCCC
    ATCGGAGGGG CTGCTGAGCT GGTGTCACAG ACTGGCTATG GTATTTTACA CGGAGCTGGA
    CTTTCTCAGC TTCCCAAACA GCGCTATCAG CCAAGTGATC TGCACGCTGA CCAGGCTCCA
    AACAGCCACG TCAAATACGT CTGGAAGATG CTCCAGTCTC TGGGCAGACC GGAAGTACAC
    ATGGCCCTGG AGGTTGTTCT GGTGAGGGGC TCGGGCCAGG AGCACGAGGG CTGCCTGCTG
    CTGACGTCAG ACGTGCTCTT CGTGGTGAGC GTCAGCGAGG ACACCCAGCA GCAGGCCTTC
    CCAGTCACGG AGATCGACTG CGCACAGGAC CGCGAGCAGA GCAGCCTGCT CATCGTGCAG
    CTCAAGCAGC CCAGAGTGGC CTGTGACGCA GAGGTGGATG GAGTTCGAGA GAGACTGTCA
    GAGCAACAGT ATAACAGACT TGTGGACTAC ATCACAAAGA CCTCTTGTCA CCTGGGCCCC
    GGCTGCTCCT CCGTGCAGGC GTCATGCCCT GTGGTGGCTG CGGAACCGCT CCCCTCCACT
    GTGAAAACAT ACCATTATAT GGTTGATCCA CATTTTGCCC AAGTTTTCAT TAGTAAATTT
    ACCATGGTGA AAAATAAAGC CCTGAGGAAA GGGTTCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120556.1
    CDS1..11862
    Translation

    Target ORF information:

    RefSeq Version XM_025264771.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GATATTAAAG ATGAAAGTGA AACAATGCTG
    AATCCTGAGG AAGTGGCATC TTTGGAGGAA TATATTCCTA CTCGACATAC AAGTGTTACT
    CTCCTCAAAT GTACCTTCAC AATTTTCATG GCTGAATTCA ATTTGCTGGA TCATTTGCTA
    CCTGTTATTA TGGGGGAAAA GAACTCAAGT AACTTCATGA ATACTACAAA CTTCCAGTCT
    CTCCGGCCAT TGCCTTCTGT TCAGATATTG GTGGATAAAA TTAATCTGGA ACATTCTGTG
    CCGATGTATG CTGAACAGTT GGTGCATGTG GTCAGCAGTC TTACTCAGCC TTCTGATAAT
    CTGCTTCATT ATTGCTATGC ACATTGCTAC CTTAAGATAT TTGGTTTCCA GGCAGGACTG
    ACGTCTTTGG ATTGTAGGGG ATCTTACTGC TTACCTGTAC CAATTGTTCC CTCTTTCAGC
    ACTGCTCTTT ATGGTAAACT TCTGAAACTA CCCACATGCT GGACCAAAAG ATCTCAGATT
    GCTATAACTG AAGGCATTTT TGAGCTTCCA AATCTTACAG TTCAAGCTAC AAGAGCTCAG
    ACACTTCTGC TTCAAGCAAT ATATCAAAGT TGGTCTCATA GTGGAAATGT CAGCTCTTTA
    GCAGTTAATG AAGCTTTGAT GAATGAAGTT TTCCAGACTG TAGGTGTGAA ATCTAAGAAT
    CCCCTGCCAA CTCTTGAGGG CTCAATCCAG AATGTTGAAT TGAAGTACTG CAGCACATCA
    TTGGTCAAAT GTGCCTCTGG GACCGTGGGA TCAATAAAAA TTTGTGCCAA AGCCCCAGGT
    GACAGTGGAA AAGAGAAGTT GATTCCATTG CTTCAGGGTC CTTCTGACAC TAAAGACCTT
    CATAGCAGCA AGTGGCTCAA TGAGAGCAGA AAGCCAGAAT CTCTCTTAGC TCCAGATTTG
    ATAGCCTTCA CAGTCCAGGT TCCACAGTGT ATGGACTACT GTCACAGTTC CGGTGCCGTA
    CTCCTTTGTA ACGTCCAAGG ACTAGCAGTT AATATTGACC CTATCTTATA CACCTGGCTC
    ATTTATCAAC CTCAGAAGCG AAGCAGTAAA CATATGCAGC AGCCTGTAAT TGCTGTTCCT
    CTTGTTATGC CAATTAGCAG AAGGAAAGAG GATGAGGTGT CTGTTGGAAG TGCACCCTTG
    GCAAAGCAAC AGTCATATCA GGCATCTGAG TATGCCAGCA GCCCTGTAAA AACAAAAACT
    GTAACAGAAT CCAGACCATT GTCAGTTCCT GTGAAAGCTG TGCTGACGAT AACTGAAGGC
    TGTAGAAGTC CTGAAGAAAG AATGAAAGAG TTTATTGGAA TAGTATGGAA TGCAGTTAAG
    AGTCTAACAC TGCAGCTTGA AGTACAGTCT TGTTGTGTGT TTATCCCAAA TGATAGTTTG
    CCTTCTCCTA GTACAATTGT ATCTGGTGAC ATTCCTGGAA CAGTAAGAAG TTGGTACCAT
    GGACAAATGA GCATGCCAGG AACGCTTGTC CTCTGTTTGC CTCAAATAAA GATTGTTAGT
    GCTGGCCACA AGTATATGGA CCCTCTCCAG GAGATTCCGT TTGTCATCCC ACGACCCATC
    CTTGAAGAAG GTGATGCTTT TCCTTGGACA ATCAGCTTGC ATCATTTCAG CATTTATACC
    CTTTTTGGAA AACAAGTGAC ACTTTGCCTA GTGGAACCCA TGGGTTGTAC CTCTACACTA
    GCTGTTACAT CCCAAAAATT GCTTGCTACA GGACCTGATA TGAGGCATTC ATTTGTTGTC
    TGTCTGCATG TTGACCTAGA GTCACTTGAG ATAAAATGCT CAAACCCCCA GGTTCAGCTC
    TTATATGAGT TAACAGATAT CATGAATGAA GTCTGGAATA AGATTCAGAA GAGAGGCAAT
    CTCAGTTCTT CTTCAGCTTA CCCGGAGTCC GTGGTGGGGC CTTTCCCTAG CTCTCCAGTT
    AGAAGCAGTA TAGGCACAGC TCCTCCAGAT AATAGCACAT GTAGCCCATC TGCTGACATT
    GGAACTACTA CTGAGGGAGA TTCTGTACAA GCTGTTGATG ATTCACCATT CTCAGATTCT
    GTTACCTTGG AACAGACTAC AAGTAACATT GGTGGATCCA GTGGTCGTGT TAGTCTGTGG
    ATGCAGTGGG TGCTCCCCAA AATTACTGTA AAACTCTTTG CTCCAGATCC TGACAATAAA
    GGCATAGAAC TTTGTATGGT CAGTGAACTA GAAGATCTCA GTGCTTCAAT AGATGTCCAG
    GATGTGTACA CTAAAGTGAA ATGTAAAATA GAAAGTTTCA ATATTGATCA CTATAGAAGC
    AACCTTGGGG AAGAGTGTTG GTCTCTGGGG CAATGTGGAG GTGTCTTCCT TTCCTGTACT
    GACAAGCTGA ACAGACGCAC CTTGTTGGTT CGACCCATCA GCAAGCAGGA CCCTTTCAGT
    AATTGCTCTG GCTTCTTTCC TTCTACAACT GCAAAGCTTC TAGATGGCTC TCATCAGCAG
    CATGGATTTC TGTCTTTGAC ATACACAAAA GCTGTAACAA AAAATGTTCG CCACAAGTTA
    ACATCAAGAA ATGAGCGAAG AAGTTTTCAT AAGTTATCTG AAGGTTTAAC AGATGGCTCC
    CCTCATTTTC TTCATGAAAT ACTTCTTTCA GCACAAGCCT TTGATATTGT CCTTTGTTTT
    CCTTTACTTA ATGCCATTGC AAGTATATTT CAAGCAAAAC TACCAAGGAC CCAAAAAGAG
    AAAAGAAAAT CTCCTGGTCA GCCCATGAGG ACCCATACAC TGACTTCACG CAATTTACCT
    TTGATTTATA TCAACACAAG CGTAATCAGA ATTTTTGTTC CAAAAACAGA AGAAATGCAG
    CCAAGTATTG AAGTTAATCA GACAGCAAAG GAAGATACCA TGGTTCTGAA GATTGGCTCT
    GTTGCTATGG CTCCCCAGGC TGACAATCCC CTTGGCAGAT CCGTCCTCAG GAAAGATATT
    TACCAAAGAG CCTTGAATTT AGGAATTCTT CGAGACCCTG GGTCAGAGAT TGAAGACCGA
    CAATACCAAA TAGATCTGCA GTCGATCAAT ATTGGTACTG CTCAGTGGGA TCAACTGAAA
    CCAGAAAAGG AAAATGGCAC AGGAGGAGTG CTAACAGAGA CTGAAAGAAA TTCGCAGAAT
    CCAGCCCTTG AGTGGAATAT GGCCAGCAGC ATACGGCGGC ATCAAGAAAG AAGAGCAATT
    TTGACTCCCA TTTTAACAGA TTTTTCTGTA CGAATAACTG GAGCACCTGC CATCATTTTC
    ACCAAAATAA TTTCTCCGGA AAATATGCAC ACTGAGGAGA TTTTAGTGTG TGGCCATTCC
    TTGGAAGTGA ATATAACCAC AAACCTGGAC TTCTTCCTAA GTGTGGCTCA AGTTCAACTC
    TTACATCAGT TAATAGTAGC AAATATGACT GGACTGGAAC CATCAAACAA AGCCACAGAG
    ATCGCTAAGC AAGAACAGAA AAAAATGGAT ACATATGATG GAATCGTGGC TGATAGCTCA
    TCTCGGTACA GTGGTGCCCA GGATAGTGGA ATCGGCAGTG ACAGCGTTAA AATCAGAATA
    GTACAGATAG AGCAGCACAG CGGTGCCAGT CAGCACCGCA TCGCCCGTCC CTCACGCCAG
    TCGTCAATTG TAAAAAATCT AAATTTTATT CCTTTTGACA TATTTATTAC TGCAAGTAGA
    ATCTCACTAA TGACCTATTC CTGTACGGCC TTACCCAAAT CGAAATTGCA AGAACAAAAG
    GATAATGAAA AAACAGGCAA GAGTTCATTA AACCTGCCAG AAGTCGATTC AGATGTCGCA
    AAACCCAGCC AGGCATGTAT TTCCATGGTA ACAGCAGAAG ATCTCTTAAG CAGCAACATA
    TCTTTTCCTT CAGGGAAAAA AATAGGGGTC ATCTCTCTCG AAAGTCTGCA TGCATCCACA
    AGGTCATCTG CTAGACAAGC CCTCGGCATA ACCATTGTTC GGCAGCCTGG AAGAAGAGGA
    ACCGGTGACT TGCGACTAGA ACCTTTCTTG TACTTTATTG TATCCCAGCC TTCTTTGCTT
    CTGAGTTGTC ACCACAGGAA GCAACGAGTG GAAATATCCA TTTTTGATGC TGTGCTTAAA
    GGGGTTGCCT CTGATTACAA ATGTACAGAT CCTGGGAAGA CTTTGCCTGA AGCCCTTGAT
    TATTGCACAG TTTGGCTGCA GACAGTACCT GGAGAAATAG ACAGCAAAAG TGGTATTCCA
    CCTTCACTCT TAACGCTACA AATTAAAGAT TTTCTTAACG GACCAGCAGA CATCAACTTG
    GATATATCAA AGCCTTTGAA AGCAAACCTG AGTTTTACCA AGTTGGATCA AATAAACCAT
    TTTTTGAAGA AGATAAAAAA TGTACACAGC AAAGCGTCCT CAGCACCGTC AGACGCTGTG
    CTGAGTAAGG ATGCGCTTCC AGCCTCCAAA TGTTACCGTG GGAAGTTATC CAAGCTTAAA
    GTTCATGGTG ATGGAGCACA AAAGATTCCA TTTCAAGAAA ACATATGGAG AACGATGTCC
    TGCTTTCAAA AAATTTCTGT TCATACTACT CAGATTGTGG TCTCCATGGA AACTGTCCCC
    CATCCTAATA AACCATGCCT GTTGGCATCT TTATCCACCC TCAGTGGAAG CCTTAATGTC
    AAAGCAGCGC AGAAAGTACC TGGTGTGATT CTTGGGTCAT CTTTTCTACT CAGCGTAAAT
    GATTTTCTTC TTAAAACGTG TCTCAAAGAA AGAAGTCGGA TTCTAATAGG ACCGTGTTGT
    GCTACTGCCA ATTTGGAAGC TAAATGGTGT AAACACAGTG GCGATCCAGG CCCAGAACAG
    TCCATACCAA AAATATCCAT TGATGTAAGA GGAGGTCTGC TACAGGTTTT CTGGGGTCAA
    GAACATTTGA ATTGCTTAAT TCTTCTACAT GAATTACTCA GTGGATACCT TAACATGGAA
    GGAAATTTTG AAGCGAAAGT TTCTGAAGCA GTTCCACAAA TGCCATCTCC TTTGGAAAAA
    AATCAGATAT TTAAAAGTGA ACAAAGTTCA GATGACCTAC GGACAGGTCT ATTTCAGTAT
    ATACGGGATG CTGAACCTCT GAAAATGCCT GGTGTCTATG AAGTCTTGTT TTACAATGAA
    ACCGAAGATA GCCCAGGGAT GATGCTGTGG AGATACCCAG AACCTAGAGT GCTCACCCTT
    GTACGCATAA CTCCTGTGCC CTTCAACACC ACCGAGGATC CAGACATTAG CACAGCAGAC
    CTTGGTGACA TGCTGCAGAT TCCTTGTAGT TTGGAATACT GGGATGAACT CCAGAAGGTT
    TTTGTTGCAT TTCGAGAATT TAGTCTGTCT GAAAGCAAAG TTTGTGAACT GCAATTGCCA
    GATATCAATC TTGTGAACGA CCAGAAGAAA CTGGTATCCT CGGATCTTTG GAGAATTGTC
    TTGAACAGCA GCCAAAATGG AGCTGATGAC CAAAGCTCTG CAAGTGAATC TGGTTCTCAA
    AGCACTTGTG ACCAGCTTGT AACCCCAACA GCTCTGGCTG CCTGTACCAG AGTTGACTCC
    TGCTTTACCC CGTGGTTTGT CCCATCTCTT TGCATTTCCT TCCAGTTTGC TCACCTGGAG
    TTCCATCTTT GTCATCACCT TGACCAGCTT GGCACAGCTC CACCAAAATA CCTACAGCCA
    TTTATTTCTG ATAAAAATGT GCCATCTGAA CTAGAATACA TGGTTATTTC CTTCAAAGAA
    CCACACATGT ATCTTCGACA GTGGAACAAT AGTTCTGTCT GTCAGGAGAT CCAGTTCTCA
    GCTCAAGCAG ACTGTAAACT TCTAGAGTGC AGAAATGTGA CAATGCAAAG CTTGGTGAAA
    CCCTTCAGCA TCTTTGGACA GACTGCAATT TCCAGCAATG CAGTAGAAAA GCTGATTGAT
    TGCTCCATCA TCATTGACTC AATATTTGTA AACTTTGGGC AACATGTAGT TCATTCTCTC
    AACACTGCAG TACAAGCTTG GCAACAGAAC AAATGCCCTG AGGTAGAGGA GTTGGTCTTC
    AGCCATTTTG TGATCTGTAA TGACACACAG GAGACACTGC GGTTTGGCCA GGTGGATACT
    GATGAAAATA TCCTGCTGGC CAGTCTCTGC AGTCACCAGT ACAGCTGGCG CTCTCACAAA
    TCCCCACAGC TGTTACACAT CTGTATTGAA GGTTGGGGCA ACTGGCGTTG GTCAGAGCCC
    TTCAGTGTGG ACCATGCGGG GACATTTATT AGAACTATTC AGTACAAGGG TCGAACTGCT
    TCACTCATCA TCAAGGCTCA GCAAGTCAGC GGCGTGCAAA AACAGATTAT CATCTGTGGA
    AGACAGATCA TCTGTAGTTA CTTGTCTCAA AGCATAGAAT TAAAAGTGGT TCAGCATTAC
    ATTGGTCAAG ATGGACAAGC TGTGGTTCGA GAACACTTTG ACTGCCTCAC AGCCAAACAG
    AAATTGCCTT CGTACATACT AGAGAACAGC GAACTGACTG AGTTGTGTGT GAGGGCCAAA
    GGAGATGAAG ACTGGTCAAG AGACGTGTGC CTGGAATCCA AGGCTCCTGA GTATAGTGTC
    GTCATTCAGG TGCCATCTTC AAACAGTTCC ATTATTTATG TCTGGTGCAC AGTTTTGACT
    TTAGAACCCA GCTCTCAAGT GCAGCAGCGA ATGATTGTGT TCAGCCCTCT TTTTATCATG
    AGGAGTCATC TTCCAGACCC CATTATCATA CATTTGGAGA AAAGGAGTCT GGGATTGAGT
    GAAACACAAA TTATTCCAGG AAAAGGACAG GAAAAACCAC TGCAAAACAT AGAACCTGAC
    CTTGTACATC ACCTAACATT TCAAGCAAGA GAAGAGGATG ACCCTTCACA CTGTGCCGTT
    CCCATCTCAA CATCCCTCAT TAAGCAGATA GCCACTAAGA TACACCCCGG AGGCACAGTT
    ACTCAGATCC TTGATGAGTT CTACGGGCCA GAGAAGTTCC TTCAACCCAC ATGGCCCTAT
    AATAAAAAGG ACTCTGACAG GAATGAGCAA CTGAGCCAGT GGGACAGCCC AATGCGAGTG
    AAGCTGTCAG CCTGGAAGCC ATATGTGAGG ACCTTATTGA TAGAATTGCT ACCCTGGGCC
    CTGCTTATCA ATCAGTCCAA GTGGGACCTC TGGCTGTTTG AGGGAGAGAA GATTGTTCTA
    CAGGTTCCTG CTGGCAAAAT TATTATCCCT CCTAATTTTC AGGAAGCTTT TCAAATTGGA
    ATATACTGGG CAAGTACAAA CACCGTGCAC AAGTCAGTAG CTGTTAAGCT GGTCCATAAC
    CTGACATCTC CAAAGTGGAA AGACGGAGGT AACGGCGAAG TTGTATCTTT GGACGAGGAA
    GGGTTTGTTC ATGCTGAAAT AAGACTCGGT GCTTTCCCAG GACACCAGAA GTTGTGCCAG
    TTCTGCATTT CCTCCATGGT ACAGCAAGGC ATACAGATTA TTCAGATTGA AGATAAGACC
    ACAATAATCA ATAACACACC ATATCAGATA TTTTATAAGC CACAGTTAAC TGTCACCAAA
    CTCCACTCTG GAAAAGAGTA TTTTCATGTT CCAGACAGCG CTACTTTCAG CATTTGCCCG
    GGTGGCGAGC AGCCTGCCGT GAGATCCAGC TCCCTTCCTT GCTGGGACCT AATGCCCGAC
    CTCGGCCAGT CCGCGCTGGA CGCGTCCCTG CTGCAGAAGC AGGTCTTGCT GGCCTTTTCT
    CCCACCGCCG TTGCCGACAG CGCCCAGTGC TGGAGCCTGC CAGCCCTAGT CAGACAGGAG
    CTTCCCAGAC AGAGCGTGGC AGTGCCCTTG GGGAACTACA GGGAAGATGG CTTCTGTACC
    AGGGCAATAG CACTAACATA TCAAGAACAC CTTGGAGTGA CTTATTTAAC CCTCTCAGAA
    GACCCTAGTC CTCGAATAAT TTTCCACAAC AGATGTCCAG TGACAATACT TATAAAGGAA
    AATACTAAAG ATACTCCGAA GTTTGAGGTT TATTGCAGAA AAATTCCTCC TGAGTGCTCA
    ATTCATCATG AGCTATATCA TCAGATTTCC AGTTATCCAG ACTGCAAGAC CAGAGACCTC
    CTTCCTAGCC TCCTCTTGAG AGTGGAATTG CTGGAGGAAA TGACAACCGA GTGGAGTGAC
    GCCATCGACA TCAACAATCA GGGGACACAG GTTGTGTTTC TGACTGGCCT TGGCTATGTG
    TACGTGGACA CCATCCATCA CTGTGGCACA GTCTTCATCA ACGTGGCCCC AGAAGGAAAA
    GCTGGGCCTG TCGTAACTAA TACCAACAGA ACTCTGGAGA AGATCGTGAC ATTTAAAATG
    TTCATCACTC AGTTGAGCCT GGCGGTGTTT GATGACCTCA CCCACCACAG AGCATCATCC
    GAGCTTTTGA GACTCACACT GGACCACGTT TTCCTCCAGA TGGCCCCCGT GGCTGGTCAC
    CTCCCTGGGG AAGAGATGGC CGCCGCCCCC TTTCACGTCT ACTCCGTGGA GGTCTGCTGC
    GGGGACCTGC AGCTGGACAA CCAGCTGTAC AACAAATCCA ATTTCCACTT TGCTGTCTTG
    GTCTGCCAGG AGGAGAAAAC AGAATCTCCT CAGTACTCCA AGATGCAGAG TCTCCTCGTA
    TCCAGTGAAG ATTTGGAAGA ATACAAGAAA AACTGCTTTA TCAAACTCTG CCTCACGTTA
    ACCGAAGGCA AGGATGTCCT GTTCGATGTG AACGAGTTCA GTTTTGAATT GAAACCTGCC
    CGGTTATATG TGGAAGACAC GTTTGTCTAC TACATCAAAA CTCTGTTTGA GACCTACCTG
    CCCAGCAGCA GCCCGGGCGC TCAGCCCACG GCTCTCGCAG GCGGCAGACA GGTGCTGCCC
    ATGCAGGTGA GGCAGCACGC CCGGGCCTTG GTGCATCCCG TGAAGCTGCA GAAGCTGGTG
    ATACAGCCTG TGAACCTCCT CGTCAGCATC CATGCGTCCC TCAAGCTGTA CATCGCCTCT
    GACCACACTC CGCTCTCCTT CTCAGTGTTT GAAAGAGGCC CCATCTTCAC CACGGCCAGG
    CAGCTCGTCC ACGCCCTGGC CATGCACTAC GCCGCAGGGG CCCTCTTCAG AGCAGGCTGG
    GTGGTTGGAT CCCTGGAAAT CCTTGGCAGC CCGGCGAGTC TGGTGCGAAG CATCGGGAAC
    GGCATCGCTG ACTTCTTCAG GCTTCCGTAC GAGGGGCTGA CCCGAGGCCC GGGAGCCTTC
    GTGAGCGGGG TTTCCAGAGG GACTACATCG TTTGTGAAGC ACATTTCCAA AGGTACCCTC
    ACGTCCATCA CCAACCTGGC CACGAGCCTG GCCCGGAACA TGGACCGGCT GTCGCTGGAC
    GAGGAGCACT ACAACCGGCA GGAAGAGTGG CGGCGGCAGC TCCCGGAGAG CCTGGGCGAG
    GGGCTCCGGC AGGGCCTGTC CCGGCTGGGT ATCAGTCTGC TCGGTGCAAT TGCTGGTATA
    GTTGATCAGC CAATGCAGAA CTTCCAGAAA ACATCAGAGG CTCAAGCTTC AGCAGGACAC
    AAGGCCAAGG GTGTTATCTC CGGCGTGGGG AAAGGAATCA TGGGGGTGTT CACGAAGCCC
    ATCGGAGGGG CTGCTGAGCT GGTGTCACAG ACTGGCTATG GTATTTTACA CGGAGCTGGA
    CTTTCTCAGC TTCCCAAACA GCGCTATCAG CCAAGTGATC TGCACGCTGA CCAGGCTCCA
    AACAGCCACG TCAAATACGT CTGGAAGATG CTCCAGTCTC TGGGCAGACC GGAAGTACAC
    ATGGCCCTGG AGGTTGTTCT GGTGAGGGGC TCGGGCCAGG AGCACGAGGG CTGCCTGCTG
    CTGACGTCAG ACGTGCTCTT CGTGGTGAGC GTCAGCGAGG ACACCCAGCA GCAGGCCTTC
    CCAGTCACGG AGATCGACTG CGCACAGGAC CGCGAGCAGA GCAGCCTGCT CATCGTGCAG
    CTCAAGCAGC CCAGAGTGGC CTGTGACGCA GAGGTGGATG GAGTTCGAGA GAGACTGTCA
    GAGCAACAGT ATAACAGACT TGTGGACTAC ATCACAAAGA CCTCTTGTCA CCTGGGCCCC
    GGCTGCTCCT CCGTGCAGGC GTCATGCCCT GTGGTGGCTG CGGAACCGCT CCCCTCCACT
    GTGAAAACAT ACCATTATAT GGTTGATCCA CATTTTGCCC AAGTTTTCAT TAGTAAATTT
    ACCATGGTGA AAAATAAAGC CCTGAGGAAA GGGTTCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49003
    Clone ID Related Accession (Same CDS sequence) XM_025264772.1
    Accession Version XM_025264772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGAAGTAAA TGAATCCTCA
    TTTCATAAGG CCTGCAGAAT GCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120557.1
    CDS1..10047
    Translation

    Target ORF information:

    RefSeq Version XM_025264772.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GCTGTTACAC ATCTGTATTG AAGGTTGGGG CAACTGGCGT
    TGGTCAGAGC CCTTCAGTGT GGACCATGCG GGGACATTTA TTAGAACTAT TCAGTACAAG
    GGTCGAACTG CTTCACTCAT CATCAAGGCT CAGCAAGTCA GCGGCGTGCA AAAACAGATT
    ATCATCTGTG GAAGACAGAT CATCTGTAGT TACTTGTCTC AAAGCATAGA ATTAAAAGTG
    GTTCAGCATT ACATTGGTCA AGATGGACAA GCTGTGGTTC GAGAACACTT TGACTGCCTC
    ACAGCCAAAC AGAAATTGCC TTCGTACATA CTAGAGAACA GCGAACTGAC TGAGTTGTGT
    GTGAGGGCCA AAGGAGATGA AGACTGGTCA AGAGACGTGT GCCTGGAATC CAAGGCTCCT
    GAGTATAGTG TCGTCATTCA GGTGCCATCT TCAAACAGTT CCATTATTTA TGTCTGGTGC
    ACAGTTTTGA CTTTAGAACC CAGCTCTCAA GTGCAGCAGC GAATGATTGT GTTCAGCCCT
    CTTTTTATCA TGAGGAGTCA TCTTCCAGAC CCCATTATCA TACATTTGGA GAAAAGGAGT
    CTGGGATTGA GTGAAACACA AATTATTCCA GGAAAAGGAC AGGAAAAACC ACTGCAAAAC
    ATAGAACCTG ACCTTGTACA TCACCTAACA TTTCAAGCAA GAGAAGAGGA TGACCCTTCA
    CACTGTGCCG TTCCCATCTC AACATCCCTC ATTAAGCAGA TAGCCACTAA GATACACCCC
    GGAGGCACAG TTACTCAGAT CCTTGATGAG TTCTACGGGC CAGAGAAGTT CCTTCAACCC
    ACATGGCCCT ATAATAAAAA GGACTCTGAC AGGAATGAGC AACTGAGCCA GTGGGACAGC
    CCAATGCGAG TGAAGCTGTC AGCCTGGAAG CCATATGTGA GGACCTTATT GATAGAATTG
    CTACCCTGGG CCCTGCTTAT CAATCAGTCC AAGTGGGACC TCTGGCTGTT TGAGGGAGAG
    AAGATTGTTC TACAGGTTCC TGCTGGCAAA ATTATTATCC CTCCTAATTT TCAGGAAGCT
    TTTCAAATTG GAATATACTG GGCAAGTACA AACACCGTGC ACAAGTCAGT AGCTGTTAAG
    CTGGTCCATA ACCTGACATC TCCAAAGTGG AAAGACGGAG GTAACGGCGA AGTTGTATCT
    TTGGACGAGG AAGGGTTTGT TCATGCTGAA ATAAGACTCG GTGCTTTCCC AGGACACCAG
    AAGTTGTGCC AGTTCTGCAT TTCCTCCATG GTACAGCAAG GCATACAGAT TATTCAGATT
    GAAGATAAGA CCACAATAAT CAATAACACA CCATATCAGA TATTTTATAA GCCACAGTTA
    ACTGTCACCA AACTCCACTC TGGAAAAGAG TATTTTCATG TTCCAGACAG CGCTACTTTC
    AGCATTTGCC CGGGTGGCGA GCAGCCTGCC GTGAGATCCA GCTCCCTTCC TTGCTGGGAC
    CTAATGCCCG ACCTCGGCCA GTCCGCGCTG GACGCGTCCC TGCTGCAGAA GCAGGTCTTG
    CTGGCCTTTT CTCCCACCGC CGTTGCCGAC AGCGCCCAGT GCTGGAGCCT GCCAGCCCTA
    GTCAGACAGG AGCTTCCCAG ACAGAGCGTG GCAGTGCCCT TGGGGAACTA CAGGGAAGAT
    GGCTTCTGTA CCAGGGCAAT AGCACTAACA TATCAAGAAC ACCTTGGAGT GACTTATTTA
    ACCCTCTCAG AAGACCCTAG TCCTCGAATA ATTTTCCACA ACAGATGTCC AGTGACAATA
    CTTATAAAGG AAAATACTAA AGATACTCCG AAGTTTGAGG TTTATTGCAG AAAAATTCCT
    CCTGAGTGCT CAATTCATCA TGAGCTATAT CATCAGATTT CCAGTTATCC AGACTGCAAG
    ACCAGAGACC TCCTTCCTAG CCTCCTCTTG AGAGTGGAAT TGCTGGAGGA AATGACAACC
    GAGTGGAGTG ACGCCATCGA CATCAACAAT CAGGGGACAC AGGAAGTAAA TGAATCCTCA
    TTTCATAAGG CCTGCAGAAT GCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49004
    Clone ID Related Accession (Same CDS sequence) XM_025264773.1
    Accession Version XM_025264773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product vacuolar protein sorting-associated protein 13B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GGTTTACAGA ATTCCAACTA CCAATGACTT AATTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025120558.1
    CDS1..8040
    Translation

    Target ORF information:

    RefSeq Version XM_025264773.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis vacuolar protein sorting 13 homolog B (VPS13B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025264773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGCTGGAGT CGTATGTGAC TCCTATTTTA ATGAGCTATG TGAATCGCTA CATCAAGAAC 
    TTAAAGCCGT CAGATCTACA GCTTTCGCTA TGGGGTGGAG ACGTGGTTCT CAGCAAGCTG
    GAGTTAAAAT TGGATGTGCT GGAGCAGGAA TTGAAATTAC CATTCACTTT TTTAAGTGGA
    CATATTCATG AACTGAGGAT CCATGTACCA TGGACAAAAC TGGGTTCAGA ACCAGTGGTA
    ATTACCATCA ATACAATGGA ATGCATTTTG AAACTTAAAG ATGGAATACA GGATGATCAT
    GAAAGCTGTG GTTCTAATTC TACCAACCGT AGTACTACTG AGAACACAAA AACATCAGCA
    AAACCCCGAA GAATTCAACA GGCCACTCCC ACAGATCCTG ATTTGCCACC AGGCTATGTA
    CAGAGTCTGA TCAGACGAGT TGTAAATAAT GTAAATATTG TTATAAATAA TCTGATACTA
    AAATATGTTG AAGATGATAT TGTCCTTTCA GTCAACATCA CATCCGCAGA ATGCTATACA
    GTAGGTGAAT TGTGGGATCG TGCATTCATG GATATTTCTG CAACTGATCT GGTGTTGAGA
    AAGGTTATCA ATTTTTCTGA CTGTACAGTT TGTCTTGATA AACGAAATGC CAGTGGTAAA
    ATAGAATTCT ACCAGGATCC TTTATTGTAC AAGTGTTCCT TCAGAACTCG TCTGCATTTT
    ACTTACGATA ACCTAAATTC TAAGATGCCA TCAGTTATAA AAATTCATAC CTTAGTAGAG
    AGTTTGAAAC TTTCTATCAC TGATCAGCAG TTGCCTATGT TTATCCGTAT AATGCAACTT
    GGAATTGCTC TTTACTATGG AGAAATAGGA AATTTTAAAG ATGGAGAAAC CGAAGATTCT
    ACTTGTCACA ATAAAGATAT GTTGGGAAAT GTTACAGGTG CTGAAGATGA AACACGAATA
    GATATGCAGT ATCCTGCTCA GTACAAAGGC CAAGAGCTGT ATTCACAGCA AGAGGACGAC
    CAGCCACAGG GATGGGTATC ATGGGCCTGG TCATTTGTCC CTGCCATTGT GAATTATGAT
    GATGCCGAGG AAGACTACCT TGGGAATGAT CCTACATCAA CTACACATCA ACAAAAAACA
    CAGACTTTGA AGGATCCTAT TGTTTCTATA GGATTTTATT GTACAAAGGC AACTGTGACT
    TTCAAACTTA CTGAAATGCA AGCCGAGAGT AGTTACTACA GCCCTCAAAA AGTAAAATCT
    AAAGAAGTAT TATGTTGGGA ACAAGAAGGA ACTACAGTTG AGGCCCTTAT GATGGGAGAG
    CCTTTCTTTG ATTGCCAGGT TGGTTTTGTA GGTTGCAGAG CCATGTGCCT TAAAGGAATT
    ATGGGTGTTA AAGATTTTGA AGAGAATATG AATAGAAGTG AAACCGAAGC CTGTTTCTTC
    CTTTGTGGTG AAAATTTGAG TATAAAAGGT TTGACATACC TCACAAATTC ATTGTTTGAT
    TACCGAAGCC CAGAAAATAA TGGTACTCGT GCAGAATTTA TCTTGGATGC AGCTACTCAT
    AAGGAGACAT ACACTGAGAT AGCTGGAATG CAAAGGTTCG GGGCATTTTA CATGGATTAC
    CTGTATACAA TGGAGAACAC CACTGGCAAA GGTTCTGCAA ATCAACAAGA CTTTTCTTCA
    GGGAAGAGTG AAGATTTGGG AACAGTTCAG GAAAAGTCTA CCAAAAGCCT TGTTATAGGT
    CCTCTTGATT TTCGCTTGGA TAGCAGTGCA GTGCACAGAA TTTTAAAAAT GGTTGTTTGT
    GCCTTGGAAC ATGAATATGA ACCATATAGT AGACTAAAAC CAGATATTAA AGATGAAAGT
    GAAACAATGC TGAATCCTGA GGAAGTGGCA TCTTTGGAGG AATATATTCC TACTCGACAT
    ACAAGTGTTA CTCTCCTCAA ATGTACCTTC ACAATTTTCA TGGCTGAATT CAATTTGCTG
    GATCATTTGC TACCTGTTAT TATGGGGGAA AAGAACTCAA GTAACTTCAT GAATACTACA
    AACTTCCAGT CTCTCCGGCC ATTGCCTTCT GTTCAGATAT TGGTGGATAA AATTAATCTG
    GAACATTCTG TGCCGATGTA TGCTGAACAG TTGGTGCATG TGGTCAGCAG TCTTACTCAG
    CCTTCTGATA ATCTGCTTCA TTATTGCTAT GCACATTGCT ACCTTAAGAT ATTTGGTTTC
    CAGGCAGGAC TGACGTCTTT GGATTGTAGG GGATCTTACT GCTTACCTGT ACCAATTGTT
    CCCTCTTTCA GCACTGCTCT TTATGGTAAA CTTCTGAAAC TACCCACATG CTGGACCAAA
    AGATCTCAGA TTGCTATAAC TGAAGGCATT TTTGAGCTTC CAAATCTTAC AGTTCAAGCT
    ACAAGAGCTC AGACACTTCT GCTTCAAGCA ATATATCAAA GTTGGTCTCA TAGTGGAAAT
    GTCAGCTCTT TAGCAGTTAA TGAAGCTTTG ATGAATGAAG TTTTCCAGAC TGTAGGTGTG
    AAATCTAAGA ATCCCCTGCC AACTCTTGAG GGCTCAATCC AGAATGTTGA ATTGAAGTAC
    TGCAGCACAT CATTGGTCAA ATGTGCCTCT GGGACCGTGG GATCAATAAA AATTTGTGCC
    AAAGCCCCAG GTGACAGTGG AAAAGAGAAG TTGATTCCAT TGCTTCAGGG TCCTTCTGAC
    ACTAAAGACC TTCATAGCAG CAAGTGGCTC AATGAGAGCA GAAAGCCAGA ATCTCTCTTA
    GCTCCAGATT TGATAGCCTT CACAGTCCAG GTTCCACAGT GTATGGACTA CTGTCACAGT
    TCCGGTGCCG TACTCCTTTG TAACGTCCAA GGACTAGCAG TTAATATTGA CCCTATCTTA
    TACACCTGGC TCATTTATCA ACCTCAGAAG CGAAGCAGTA AACATATGCA GCAGCCTGTA
    ATTGCTGTTC CTCTTGTTAT GCCAATTAGC AGAAGGAAAG AGGATGAGGT GTCTGTTGGA
    AGTGCACCCT TGGCAAAGCA ACAGTCATAT CAGGCATCTG AGTATGCCAG CAGCCCTGTA
    AAAACAAAAA CTGTAACAGA ATCCAGACCA TTGTCAGTTC CTGTGAAAGC TGTGCTGACG
    ATAACTGAAG GCTGTAGAAG TCCTGAAGAA AGAATGAAAG AGTTTATTGG AATAGTATGG
    AATGCAGTTA AGAGTCTAAC ACTGCAGCTT GAAGTACAGT CTTGTTGTGT GTTTATCCCA
    AATGATAGTT TGCCTTCTCC TAGTACAATT GTATCTGGTG ACATTCCTGG AACAGTAAGA
    AGTTGGTACC ATGGACAAAT GAGCATGCCA GGAACGCTTG TCCTCTGTTT GCCTCAAATA
    AAGATTGTTA GTGCTGGCCA CAAGTATATG GACCCTCTCC AGGAGATTCC GTTTGTCATC
    CCACGACCCA TCCTTGAAGA AGGTGATGCT TTTCCTTGGA CAATCAGCTT GCATCATTTC
    AGCATTTATA CCCTTTTTGG AAAACAAGTG ACACTTTGCC TAGTGGAACC CATGGGTTGT
    ACCTCTACAC TAGCTGTTAC ATCCCAAAAA TTGCTTGCTA CAGGACCTGA TATGAGGCAT
    TCATTTGTTG TCTGTCTGCA TGTTGACCTA GAGTCACTTG AGATAAAATG CTCAAACCCC
    CAGGTTCAGC TCTTATATGA GTTAACAGAT ATCATGAATG AAGTCTGGAA TAAGATTCAG
    AAGAGAGGCA ATCTCAGTTC TTCTTCAGCT TACCCGGAGT CCGTGGTGGG GCCTTTCCCT
    AGCTCTCCAG TTAGAAGCAG TATAGGCACA GCTCCTCCAG ATAATAGCAC ATGTAGCCCA
    TCTGCTGACA TTGGAACTAC TACTGAGGGA GATTCTGTAC AAGCTGTTGA TGATTCACCA
    TTCTCAGATT CTGTTACCTT GGAACAGACT ACAAGTAACA TTGGTGGATC CAGTGGTCGT
    GTTAGTCTGT GGATGCAGTG GGTGCTCCCC AAAATTACTG TAAAACTCTT TGCTCCAGAT
    CCTGACAATA AAGGCATAGA ACTTTGTATG GTCAGTGAAC TAGAAGATCT CAGTGCTTCA
    ATAGATGTCC AGGATGTGTA CACTAAAGTG AAATGTAAAA TAGAAAGTTT CAATATTGAT
    CACTATAGAA GCAACCTTGG GGAAGAGTGT TGGTCTCTGG GGCAATGTGG AGGTGTCTTC
    CTTTCCTGTA CTGACAAGCT GAACAGACGC ACCTTGTTGG TTCGACCCAT CAGCAAGCAG
    GACCCTTTCA GTAATTGCTC TGGCTTCTTT CCTTCTACAA CTGCAAAGCT TCTAGATGGC
    TCTCATCAGC AGCATGGATT TCTGTCTTTG ACATACACAA AAGCTGTAAC AAAAAATGTT
    CGCCACAAGT TAACATCAAG AAATGAGCGA AGAAGTTTTC ATAAGTTATC TGAAGGTTTA
    ACAGATGGCT CCCCTCATTT TCTTCATGAA ATACTTCTTT CAGCACAAGC CTTTGATATT
    GTCCTTTGTT TTCCTTTACT TAATGCCATT GCAAGTATAT TTCAAGCAAA ACTACCAAGG
    ACCCAAAAAG AGAAAAGAAA ATCTCCTGGT CAGCCCATGA GGACCCATAC ACTGACTTCA
    CGCAATTTAC CTTTGATTTA TATCAACACA AGCGTAATCA GAATTTTTGT TCCAAAAACA
    GAAGAAATGC AGCCAAGTAT TGAAGTTAAT CAGACAGCAA AGGAAGATAC CATGGTTCTG
    AAGATTGGCT CTGTTGCTAT GGCTCCCCAG GCTGACAATC CCCTTGGCAG ATCCGTCCTC
    AGGAAAGATA TTTACCAAAG AGCCTTGAAT TTAGGAATTC TTCGAGACCC TGGGTCAGAG
    ATTGAAGACC GACAATACCA AATAGATCTG CAGTCGATCA ATATTGGTAC TGCTCAGTGG
    GATCAACTGA AACCAGAAAA GGAAAATGGC ACAGGAGGAG TGCTAACAGA GACTGAAAGA
    AATTCGCAGA ATCCAGCCCT TGAGTGGAAT ATGGCCAGCA GCATACGGCG GCATCAAGAA
    AGAAGAGCAA TTTTGACTCC CATTTTAACA GATTTTTCTG TACGAATAAC TGGAGCACCT
    GCCATCATTT TCACCAAAAT AATTTCTCCG GAAAATATGC ACACTGAGGA GATTTTAGTG
    TGTGGCCATT CCTTGGAAGT GAATATAACC ACAAACCTGG ACTTCTTCCT AAGTGTGGCT
    CAAGTTCAAC TCTTACATCA GTTAATAGTA GCAAATATGA CTGGACTGGA ACCATCAAAC
    AAAGCCACAG AGATCGCTAA GCAAGAACAG AAAAAAATGG ATACATATGA TGGAATCGTG
    GCTGATAGCT CATCTCGGTA CAGTGGTGCC CAGGATAGTG GAATCGGCAG TGACAGCGTT
    AAAATCAGAA TAGTACAGAT AGAGCAGCAC AGCGGTGCCA GTCAGCACCG CATCGCCCGT
    CCCTCACGCC AGTCGTCAAT TGTAAAAAAT CTAAATTTTA TTCCTTTTGA CATATTTATT
    ACTGCAAGTA GAATCTCACT AATGACCTAT TCCTGTACGG CCTTACCCAA ATCGAAATTG
    CAAGAACAAA AGGATAATGA AAAAACAGGC AAGAGTTCAT TAAACCTGCC AGAAGTCGAT
    TCAGATGTCG CAAAACCCAG CCAGGCATGT ATTTCCATGG TAACAGCAGA AGATCTCTTA
    AGCAGCAACA TATCTTTTCC TTCAGGGAAA AAAATAGGGG TCATCTCTCT CGAAAGTCTG
    CATGCATCCA CAAGGTCATC TGCTAGACAA GCCCTCGGCA TAACCATTGT TCGGCAGCCT
    GGAAGAAGAG GAACCGGTGA CTTGCGACTA GAACCTTTCT TGTACTTTAT TGTATCCCAG
    CCTTCTTTGC TTCTGAGTTG TCACCACAGG AAGCAACGAG TGGAAATATC CATTTTTGAT
    GCTGTGCTTA AAGGGGTTGC CTCTGATTAC AAATGTACAG ATCCTGGGAA GACTTTGCCT
    GAAGCCCTTG ATTATTGCAC AGTTTGGCTG CAGACAGTAC CTGGAGAAAT AGACAGCAAA
    AGTGGTATTC CACCTTCACT CTTAACGCTA CAAATTAAAG ATTTTCTTAA CGGACCAGCA
    GACATCAACT TGGATATATC AAAGCCTTTG AAAGCAAACC TGAGTTTTAC CAAGTTGGAT
    CAAATAAACC ATTTTTTGAA GAAGATAAAA AATGTACACA GCAAAGCGTC CTCAGCACCG
    TCAGACGCTG TGCTGAGTAA GGATGCGCTT CCAGCCTCCA AATGTTACCG TGGGAAGTTA
    TCCAAGCTTA AAGTTCATGG TGATGGAGCA CAAAAGATTC CATTTCAAGA AAACATATGG
    AGAACGATGT CCTGCTTTCA AAAAATTTCT GTTCATACTA CTCAGATTGT GGTCTCCATG
    GAAACTGTCC CCCATCCTAA TAAACCATGC CTGTTGGCAT CTTTATCCAC CCTCAGTGGA
    AGCCTTAATG TCAAAGCAGC GCAGAAAGTA CCTGGTGTGA TTCTTGGGTC ATCTTTTCTA
    CTCAGCGTAA ATGATTTTCT TCTTAAAACG TGTCTCAAAG AAAGAAGTCG GATTCTAATA
    GGACCGTGTT GTGCTACTGC CAATTTGGAA GCTAAATGGT GTAAACACAG TGGCGATCCA
    GGCCCAGAAC AGTCCATACC AAAAATATCC ATTGATGTAA GAGGAGGTCT GCTACAGGTT
    TTCTGGGGTC AAGAACATTT GAATTGCTTA ATTCTTCTAC ATGAATTACT CAGTGGATAC
    CTTAACATGG AAGGAAATTT TGAAGCGAAA GTTTCTGAAG CAGTTCCACA AATGCCATCT
    CCTTTGGAAA AAAATCAGAT ATTTAAAAGT GAACAAAGTT CAGATGACCT ACGGACAGGT
    CTATTTCAGT ATATACGGGA TGCTGAACCT CTGAAAATGC CTGGTGTCTA TGAAGTCTTG
    TTTTACAATG AAACCGAAGA TAGCCCAGGG ATGATGCTGT GGAGATACCC AGAACCTAGA
    GTGCTCACCC TTGTACGCAT AACTCCTGTG CCCTTCAACA CCACCGAGGA TCCAGACATT
    AGCACAGCAG ACCTTGGTGA CATGCTGCAG ATTCCTTGTA GTTTGGAATA CTGGGATGAA
    CTCCAGAAGG TTTTTGTTGC ATTTCGAGAA TTTAGTCTGT CTGAAAGCAA AGTTTGTGAA
    CTGCAATTGC CAGATATCAA TCTTGTGAAC GACCAGAAGA AACTGGTATC CTCGGATCTT
    TGGAGAATTG TCTTGAACAG CAGCCAAAAT GGAGCTGATG ACCAAAGCTC TGCAAGTGAA
    TCTGGTTCTC AAAGCACTTG TGACCAGCTT GTAACCCCAA CAGCTCTGGC TGCCTGTACC
    AGAGTTGACT CCTGCTTTAC CCCGTGGTTT GTCCCATCTC TTTGCATTTC CTTCCAGTTT
    GCTCACCTGG AGTTCCATCT TTGTCATCAC CTTGACCAGC TTGGCACAGC TCCACCAAAA
    TACCTACAGC CATTTATTTC TGATAAAAAT GTGCCATCTG AACTAGAATA CATGGTTATT
    TCCTTCAAAG AACCACACAT GTATCTTCGA CAGTGGAACA ATAGTTCTGT CTGTCAGGAG
    ATCCAGTTCT CAGCTCAAGC AGACTGTAAA CTTCTAGAGT GCAGAAATGT GACAATGCAA
    AGCTTGGTGA AACCCTTCAG CATCTTTGGA CAGACTGCAA TTTCCAGCAA TGCAGTAGAA
    AAGCTGATTG ATTGCTCCAT CATCATTGAC TCAATATTTG TAAACTTTGG GCAACATGTA
    GTTCATTCTC TCAACACTGC AGTACAAGCT TGGCAACAGA ACAAATGCCC TGAGGTAGAG
    GAGTTGGTCT TCAGCCATTT TGTGATCTGT AATGACACAC AGGAGACACT GCGGTTTGGC
    CAGGTGGATA CTGATGAAAA TATCCTGCTG GCCAGTCTCT GCAGTCACCA GTACAGCTGG
    CGCTCTCACA AATCCCCACA GGTTTACAGA ATTCCAACTA CCAATGACTT AATTTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.