CFAP74 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006044662.2	XP_006044724.1	cilia- and flagella-associated protein 74 isoform X1
XM_006044665.1	XP_006044727.1	uncharacterized protein KIAA1751 homolog isoform X4
XM_006044668.1	XP_006044730.1	uncharacterized protein KIAA1751 homolog isoform X7
XM_006044664.2	XP_006044726.1	cilia- and flagella-associated protein 74 isoform X3
XM_006044662.1	XP_006044724.1	uncharacterized protein KIAA1751 homolog isoform X1
XM_006044667.1	XP_006044729.1	uncharacterized protein KIAA1751 homolog isoform X6
XM_025285761.1	XP_025141546.1	cilia- and flagella-associated protein 74 isoform X1
XM_006044663.2	XP_006044725.1	cilia- and flagella-associated protein 74 isoform X2
XM_006044665.2	XP_006044727.1	cilia- and flagella-associated protein 74 isoform X4
XM_006044663.1	XP_006044725.1	uncharacterized protein KIAA1751 homolog isoform X2
XM_006044664.1	XP_006044726.1	uncharacterized protein KIAA1751 homolog isoform X3
XM_006044666.1	XP_006044728.1	uncharacterized protein KIAA1751 homolog isoform X5
XM_025285762.1	XP_025141547.1	cilia- and flagella-associated protein 74 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu03805	XM_006044667.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu03806	XM_006044668.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu66183	XM_025285762.1 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu03800	XM_006044662.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu03801	XM_006044663.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu03802	XM_006044664.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu03803	XM_006044665.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu03804	XM_006044666.1 Latest version!	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu66179	XM_025285761.1 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu66179	XM_006044662.2 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu66180	XM_006044663.2 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu66181	XM_006044664.2 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu66182	XM_006044665.2 Latest version!	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

CFAP74 ( XM_006044667.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044729.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu03805
Clone ID Related Accession (Same CDS sequence)	XM_006044667.1
Accession Version	XM_006044667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGTGTCCC 
ACCACATCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044729.1
CDS	115..4569
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKCPTTSS

Target ORF information:

RefSeq Version	XM_006044667.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044667.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGTGTCCC 
ACCACATCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044668.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044730.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu03806
Clone ID Related Accession (Same CDS sequence)	XM_006044668.1
Accession Version	XM_006044668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC GCATGGACGC GGTGCTGGCC CTGAAGAACA GTATCTCCGC CAACCGGGAA 
ACGCTCCGGA AATTTCAAGC ATGGGGCCAA GCCAAGACAG AGTTGGCAGA ACAGAAGGCC 
CAGGCCGAGA AGAAGGCAAT TCTGGCCCAA GGTGGGGACG CCTTTAAGCA CCTGTGCCAT 
CAGCGCCGGC GCCAGGAGTT GGAGGCCCAG AACCGCGCCT TCGAGGAGGA GCAGAAGCGC 
AGGAAGCAGG AGATTGTGAG TCGGCTCTTG AAGGAAGAGG CCATGGAGGA CCGGAGGAGG 
GCACGGCCCG AGCCCCCCAG ACCCGCAGGC CAGCGGAGCC TGAGGGACCA GACCTGGAGC 
TACGTGGCCA CCTTCTGCGA TGGGCAGAGC ACAGAGGCCC CGCTCTGCTG TCCCCTGCCT 
GATGTCCCTG GACAGAGGCC AGGTCCAGCC CAAGAGCAGA TAAAGCTCCA GGAGACCAGG 
TCCAGATTCT GCCAGTCCTC TCCATTGCTG ACGGCCATCT CCAGTGAGTC TGTCCAGGGG 
GACGGGAGCC TCGGCGGGGA GGACACACTG GCTTGGCCGG AGATCCCCGG GCTCTGGAAT 
GAGGACTACA AGCCTTTCCA GGTATCCAAG GAGGATGTGG ACCAGAAGCC GGTGGGTGGA 
ACAAAGATGG ACAAGGACAT CCTGGCGCGG ACAATGCAAC GACTACGCAG TGGGGTGATC 
CACAAACAGG TGGCATCAGG GCGTGAGTTC AGAGGGTGCC CCTTCAACAG CAAGCCCAAG 
TTCATCCACT TCAAGGACTT TGATGTGGGC AAAGTGTACA AGAAGAAGAT CACGCTGATC 
AATGCCACCT ACACGATTAA CTACTGCAAG CTGGTGGGCG TGGACGAGAG CCTGCAGGAC 
TTCATCCACG TGGACTTTGA CCCCCCGGGC CCACTATCAG CCGGCATGTC CTGCGAAGTG 
CTTGTCACCT TCAAGCCCAT GATAAACAAA GATCTGGAAG GAAGTGTCTC GTTTCTGGCT 
CAGACAGGCG GATTTTCTGT TCCACTGAAA TGTTCAACAA AGAAATGCTC TCTGTCTCTC 
GACAAGGAGC TCATCGACTT TGGCAGCTAC ACAGTGGGTG AGACTGCAAT CCGGACCATC 
ACGCTGACCA ACACTGGGGG CTTGGGCACC AAGTTCAGAT TTCTTCTGGC ATCAGAGACT 
TGTGAGATGG ACACCTCCCA GTCGGTCCTA AAATTAAGTA GTACCTTCAC GTATGAAGAC 
AAAAGTTTGT ATGACAAATT CACGACCAGT TTTTCTGAGC ATCAGATGGA GGGCAATGAG 
TCATCTCCTA CAGATGTACC AAGCCAGAAG GAGTCTGGAA AGCTGGACGT GAAGGAGCAG 
GAGGAGGGGC ACCCCATCGA ATCAGAGGGC GTGACCTTGA CCACCATCAT GACTATACCT 
CCCAATGAGG AACAGATGGA GTTCTCGCTG GGAGAGACAA CAGAGGGGGA CATAGGCCCC 
TTCAGCTCCA TCAAGGTGCC CGTCATCTTC ACCCCGATTG TCCCGGGAAC GGTCCAGAAC 
AGTTTCAAGG TTGTGTTTAA GAATCAGCAG TGCCCAACTT TATATTTCAA AGTCGTTGGC 
GTGGCTATCG ATGTGCCTGT CTGGGTACCA AAGCCCAACG TAGACCTGAA GATCTGCATG 
TACGATCGGC TGTACCAGGA CTCGATCCTC GTCCACACAC GGTCGAAGGC CGCGTTGCGC 
CTAAAGTTCG AGGTGTGCAA GGAGCTGCGG GGCCACATGG AGCTCCTGCC TGAGACTGGC 
TACATCCAGG CCCTGTCGTC CTACTCAGTG CAGCTCAAGT TCCTGCCCAG ACACTCCCTC 
CCAGAAGACG CAGGGAAGTA TTTTGACAAG GAGACCAGGG TTCTGGAGGC CCCGATGACT 
ATCCGGGTGG CTGACCAGAT CAAACCCGTG GGGTTTACTG TCCATGCTAT AGTCACCACC 
TCGGACCTGG AGCTCAGCCC CGCAGGGCTG GACTTTGGCT ACTGTACCAT CTACGAGGCC 
ATCAGGACCC AAATCAGCCT CTGCAATCAT TCCCTCCTGC CCCAGGAGTT TGGGTTCGTC 
GGACTCCCCA AGTTTGTGGA CATCCAGCCC AACGACGGGT TCGGGACAAT CCTGCCACTG 
GAAACGCTAC AGCTCGATGT GATCTTTCAG CCCACCAAGG CCAAGGAGTA CAACTTTGAG 
CTCATCTGCA AGTCAGAGAT TAATCGGTGC TTCAAGCTGG CATGCCGGGC CGTGGGTGTC 
TACCCGCCCC TGGAGCTGTC CCACAACCAG ATCAAGTTTG CAGCCACCTC CTTGTACGAC 
ACATCCGTGG CCACCCTGTA TGTCATCAAC TCCCACTTGA GCATGAACTC ACTGACCCAC 
TCAGTGCCCC GCATTGGCTC GGAGGATGCT TTTCCCGTGG GGCCCACGTC TTTTGAGTTC 
CTGCTGCCCC CAGATTCGCC CATCACCATC TCACCCCTGG TGGGCACCGT GTGGCCAGGA 
AAGAGGTGCC TGGTCCGAGT GGCCTTCCGG CCAGTGCTGC CCAGCGAGCT GATCCACCAG 
GAGGCCTTCC AGGCCCTGAG CAGAAAAGCT GAGGTCAAAT CATCCCAAAA GGAGACGGTC 
ACCCAGAGGA AGGACCAGCG GAGACCATCT TTCTCCATGC TGCGGCTGCA GAACCAGGAC 
CAGCTGCTCC GGGCCTCAGC CTCCCAGCCT CCGGAGCTGC AGAAGGAGGA CCTCGCATCC 
AGCTCTCAAG AGTACCAGTC GGCACAGGCC TCCCTGACCA GGTCTTTCCA GGGGAAGTTT 
GACAAGTTTG TGGTTCCCTG TGTTGTTGCT AGTGGCGACA CCAAAGACAG GAAGGGGACA 
GAACCGCTGA GCTTCAGTCC CCACAACACG CTGTACCTGG AGCTGTGGTG TCCAGCGGTG 
GCGCCGTCTA TCGTGGTGAC GTCTGACAAA GGCAAGACCA TCATTAACTT CGGCGACATT 
GCTGCAGGAC ACCGGGGCAT AAAGAAGGTC TCCATCCAGA ACATCTCCCC TGAGGATCTC 
GACCTGGAGT ACTCCGTGCT GAACCCCGAT GGCCCCTTCG TCCTGCTGAA CCCCACCATC 
AGGTTGCCCT CCGGCGAGAC CCAGGTCCTA GATCTGTCCT TCTCCCCACG TGAGAGTGTC 
GTGGCCCAAG AAACACTGGA CATCATCACC AAGAAAGGCA CCCTCTCCCT GGCCCTCATG 
GGCACGGGTG TGGCGTCCAC GATCACGTGC TCCATTGAAG GTGATGTCCT CAACATGGGC 
TACGTGATTG CCAGAGAGTC TGTCTCCTTG GGCTTCAAGC TGCAGAACAA CTCCTCACTG 
CCCATCAAGT TCTCCATGCA GCTGGACAGC CTCTCCTCCA GGAGCAGAGA CCAGCACCGC 
CTGCCACAGT TCCTGGCCTC GCCTGACCAG AGGACGGAAG TCGTGGGCAC GCAGAACTAC 
AGTGGCCAGA GCGTGTTCAG CGTGGTCCCG GTCGAGGGTG TCATGGAGCC CGGCAAGGCT 
CAGGACTTCA CTGTGACCTT CAGCCCAGAC CACGAGAGCC TGTACTTCTC TGACCGGCTG 
CAGGTCGTGC TCTTTGAAAA GAAAGTGTCC CACCACATCC TCCTGAAGGG CGCAGCCCGC 
GAGCACATGA TGTTCGTGGA GGGCGGAGAC CCCCTGGACG TGCCCGTGGA GTCCCTGACC 
GTGATCCCTG CCTTTGACCT GGAGCACAGA GAGGGCCCTC CCTGTCCAGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ATCCAGAAAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CACAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACGCCA 
CCCATCGACT TTGATCCAGA CCACCCACTC TTGACATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044730.1
CDS	455..4591
Translation	MRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044668.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044668.1

ORF Insert Sequence:

ATGCGCAGGC GCATGGACGC GGTGCTGGCC CTGAAGAACA GTATCTCCGC CAACCGGGAA 
ACGCTCCGGA AATTTCAAGC ATGGGGCCAA GCCAAGACAG AGTTGGCAGA ACAGAAGGCC 
CAGGCCGAGA AGAAGGCAAT TCTGGCCCAA GGTGGGGACG CCTTTAAGCA CCTGTGCCAT 
CAGCGCCGGC GCCAGGAGTT GGAGGCCCAG AACCGCGCCT TCGAGGAGGA GCAGAAGCGC 
AGGAAGCAGG AGATTGTGAG TCGGCTCTTG AAGGAAGAGG CCATGGAGGA CCGGAGGAGG 
GCACGGCCCG AGCCCCCCAG ACCCGCAGGC CAGCGGAGCC TGAGGGACCA GACCTGGAGC 
TACGTGGCCA CCTTCTGCGA TGGGCAGAGC ACAGAGGCCC CGCTCTGCTG TCCCCTGCCT 
GATGTCCCTG GACAGAGGCC AGGTCCAGCC CAAGAGCAGA TAAAGCTCCA GGAGACCAGG 
TCCAGATTCT GCCAGTCCTC TCCATTGCTG ACGGCCATCT CCAGTGAGTC TGTCCAGGGG 
GACGGGAGCC TCGGCGGGGA GGACACACTG GCTTGGCCGG AGATCCCCGG GCTCTGGAAT 
GAGGACTACA AGCCTTTCCA GGTATCCAAG GAGGATGTGG ACCAGAAGCC GGTGGGTGGA 
ACAAAGATGG ACAAGGACAT CCTGGCGCGG ACAATGCAAC GACTACGCAG TGGGGTGATC 
CACAAACAGG TGGCATCAGG GCGTGAGTTC AGAGGGTGCC CCTTCAACAG CAAGCCCAAG 
TTCATCCACT TCAAGGACTT TGATGTGGGC AAAGTGTACA AGAAGAAGAT CACGCTGATC 
AATGCCACCT ACACGATTAA CTACTGCAAG CTGGTGGGCG TGGACGAGAG CCTGCAGGAC 
TTCATCCACG TGGACTTTGA CCCCCCGGGC CCACTATCAG CCGGCATGTC CTGCGAAGTG 
CTTGTCACCT TCAAGCCCAT GATAAACAAA GATCTGGAAG GAAGTGTCTC GTTTCTGGCT 
CAGACAGGCG GATTTTCTGT TCCACTGAAA TGTTCAACAA AGAAATGCTC TCTGTCTCTC 
GACAAGGAGC TCATCGACTT TGGCAGCTAC ACAGTGGGTG AGACTGCAAT CCGGACCATC 
ACGCTGACCA ACACTGGGGG CTTGGGCACC AAGTTCAGAT TTCTTCTGGC ATCAGAGACT 
TGTGAGATGG ACACCTCCCA GTCGGTCCTA AAATTAAGTA GTACCTTCAC GTATGAAGAC 
AAAAGTTTGT ATGACAAATT CACGACCAGT TTTTCTGAGC ATCAGATGGA GGGCAATGAG 
TCATCTCCTA CAGATGTACC AAGCCAGAAG GAGTCTGGAA AGCTGGACGT GAAGGAGCAG 
GAGGAGGGGC ACCCCATCGA ATCAGAGGGC GTGACCTTGA CCACCATCAT GACTATACCT 
CCCAATGAGG AACAGATGGA GTTCTCGCTG GGAGAGACAA CAGAGGGGGA CATAGGCCCC 
TTCAGCTCCA TCAAGGTGCC CGTCATCTTC ACCCCGATTG TCCCGGGAAC GGTCCAGAAC 
AGTTTCAAGG TTGTGTTTAA GAATCAGCAG TGCCCAACTT TATATTTCAA AGTCGTTGGC 
GTGGCTATCG ATGTGCCTGT CTGGGTACCA AAGCCCAACG TAGACCTGAA GATCTGCATG 
TACGATCGGC TGTACCAGGA CTCGATCCTC GTCCACACAC GGTCGAAGGC CGCGTTGCGC 
CTAAAGTTCG AGGTGTGCAA GGAGCTGCGG GGCCACATGG AGCTCCTGCC TGAGACTGGC 
TACATCCAGG CCCTGTCGTC CTACTCAGTG CAGCTCAAGT TCCTGCCCAG ACACTCCCTC 
CCAGAAGACG CAGGGAAGTA TTTTGACAAG GAGACCAGGG TTCTGGAGGC CCCGATGACT 
ATCCGGGTGG CTGACCAGAT CAAACCCGTG GGGTTTACTG TCCATGCTAT AGTCACCACC 
TCGGACCTGG AGCTCAGCCC CGCAGGGCTG GACTTTGGCT ACTGTACCAT CTACGAGGCC 
ATCAGGACCC AAATCAGCCT CTGCAATCAT TCCCTCCTGC CCCAGGAGTT TGGGTTCGTC 
GGACTCCCCA AGTTTGTGGA CATCCAGCCC AACGACGGGT TCGGGACAAT CCTGCCACTG 
GAAACGCTAC AGCTCGATGT GATCTTTCAG CCCACCAAGG CCAAGGAGTA CAACTTTGAG 
CTCATCTGCA AGTCAGAGAT TAATCGGTGC TTCAAGCTGG CATGCCGGGC CGTGGGTGTC 
TACCCGCCCC TGGAGCTGTC CCACAACCAG ATCAAGTTTG CAGCCACCTC CTTGTACGAC 
ACATCCGTGG CCACCCTGTA TGTCATCAAC TCCCACTTGA GCATGAACTC ACTGACCCAC 
TCAGTGCCCC GCATTGGCTC GGAGGATGCT TTTCCCGTGG GGCCCACGTC TTTTGAGTTC 
CTGCTGCCCC CAGATTCGCC CATCACCATC TCACCCCTGG TGGGCACCGT GTGGCCAGGA 
AAGAGGTGCC TGGTCCGAGT GGCCTTCCGG CCAGTGCTGC CCAGCGAGCT GATCCACCAG 
GAGGCCTTCC AGGCCCTGAG CAGAAAAGCT GAGGTCAAAT CATCCCAAAA GGAGACGGTC 
ACCCAGAGGA AGGACCAGCG GAGACCATCT TTCTCCATGC TGCGGCTGCA GAACCAGGAC 
CAGCTGCTCC GGGCCTCAGC CTCCCAGCCT CCGGAGCTGC AGAAGGAGGA CCTCGCATCC 
AGCTCTCAAG AGTACCAGTC GGCACAGGCC TCCCTGACCA GGTCTTTCCA GGGGAAGTTT 
GACAAGTTTG TGGTTCCCTG TGTTGTTGCT AGTGGCGACA CCAAAGACAG GAAGGGGACA 
GAACCGCTGA GCTTCAGTCC CCACAACACG CTGTACCTGG AGCTGTGGTG TCCAGCGGTG 
GCGCCGTCTA TCGTGGTGAC GTCTGACAAA GGCAAGACCA TCATTAACTT CGGCGACATT 
GCTGCAGGAC ACCGGGGCAT AAAGAAGGTC TCCATCCAGA ACATCTCCCC TGAGGATCTC 
GACCTGGAGT ACTCCGTGCT GAACCCCGAT GGCCCCTTCG TCCTGCTGAA CCCCACCATC 
AGGTTGCCCT CCGGCGAGAC CCAGGTCCTA GATCTGTCCT TCTCCCCACG TGAGAGTGTC 
GTGGCCCAAG AAACACTGGA CATCATCACC AAGAAAGGCA CCCTCTCCCT GGCCCTCATG 
GGCACGGGTG TGGCGTCCAC GATCACGTGC TCCATTGAAG GTGATGTCCT CAACATGGGC 
TACGTGATTG CCAGAGAGTC TGTCTCCTTG GGCTTCAAGC TGCAGAACAA CTCCTCACTG 
CCCATCAAGT TCTCCATGCA GCTGGACAGC CTCTCCTCCA GGAGCAGAGA CCAGCACCGC 
CTGCCACAGT TCCTGGCCTC GCCTGACCAG AGGACGGAAG TCGTGGGCAC GCAGAACTAC 
AGTGGCCAGA GCGTGTTCAG CGTGGTCCCG GTCGAGGGTG TCATGGAGCC CGGCAAGGCT 
CAGGACTTCA CTGTGACCTT CAGCCCAGAC CACGAGAGCC TGTACTTCTC TGACCGGCTG 
CAGGTCGTGC TCTTTGAAAA GAAAGTGTCC CACCACATCC TCCTGAAGGG CGCAGCCCGC 
GAGCACATGA TGTTCGTGGA GGGCGGAGAC CCCCTGGACG TGCCCGTGGA GTCCCTGACC 
GTGATCCCTG CCTTTGACCT GGAGCACAGA GAGGGCCCTC CCTGTCCAGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ATCCAGAAAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CACAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACGCCA 
CCCATCGACT TTGATCCAGA CCACCCACTC TTGACATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_025285762.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141547.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.

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CloneID	OBu66183
Clone ID Related Accession (Same CDS sequence)	XM_025285762.1
Accession Version	XM_025285762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAAG GGATCCGCAA GGGAAGCCCT GGAGGATTTT CAGGAGAGGC AGCTGGGAGT 
GCATGGGGAG TGGTGATGAC GTCCTCACCA CCTGTGTCTC TGGAAACGCT CCGGAAATTT 
CAAGCATGGG GCCAAGCCAA GACAGAGTTG GCAGAACAGA AGGCCCAGGC CGAGAAGAAG 
GCAATTCTGG CCCAAGGTGG GGACGCCTTT AAGCACCTGT GCCATCAGCG CCGGCGCCAG 
GAGTTGGAGG CCCAGAACCG CGCCTTCGAG GAGGAGCAGA AGCGCAGGAA GCAGGAGATT 
GTGAGTCGGC TCTTGAAGGA AGAGGCCATG GAGGACCGGA GGAGGGCACG GCCCGAGCCC 
CCCAGACCCG CAGGCCAGCG GAGCCTGAGG GACCAGACCT GGAGCTACGT GGCCACCTTC 
TGCGATGGGC AGAGCACAGA GGCCCCGCTC TGCTGTCCCC TGCCTGATGT CCCTGGACAG 
AGGCCAGGTC CAGCCCAAGA GCAGATAAAG CTCCAGGAGA CCAGGTCCAG ATTCTGCCAG 
TCCTCTCCAT TGCTGACGGC CATCTCCAGT GAGTCTGTCC AGGGGGACGG GAGCCTCGGC 
GGGGAGGACA CACTGGCTTG GCCGGAGATC CCCGGGCTCT GGAATGAGGA CTACAAGCCT 
TTCCAGGTAT CCAAGGAGGA TGTGGACCAG AAGCCGGTGG GTGGAACAAA GATGGACAAG 
GACATCCTGG CGCGGACAAT GCAACGACTA CGCAGTGGGG TGATCCACAA ACAGGTGGCA 
TCAGGGCGTG AGTTCAGAGG GTGCCCCTTC AACAGCAAGC CCAAGTTCAT CCACTTCAAG 
GACTTTGATG TGGGCAAAGT GTACAAGAAG AAGATCACGC TGATCAATGC CACCTACACG 
ATTAACTACT GCAAGCTGGT GGGCGTGGAC GAGAGCCTGC AGGACTTCAT CCACGTGGAC 
TTTGACCCCC CGGGCCCACT ATCAGCCGGC ATGTCCTGCG AAGTGCTTGT CACCTTCAAG 
CCCATGATAA ACAAAGATCT GGAAGGAAGT GTCTCGTTTC TGGCTCAGAC AGGCGGATTT 
TCTGTTCCAC TGAAATGTTC AACAAAGAAA TGCTCTCTGT CTCTCGACAA GGAGCTCATC 
GACTTTGGCA GCTACACAGT GGGTGAGACT GCAATCCGGA CCATCACGCT GACCAACACT 
GGGGGCTTGG GCACCAAGTT CAGATTTCTT CTGGCATCAG AGACTTGTGA GATGGACACC 
TCCCAGTCGG TCCTAAAATT AAGTAGTACC TTCACGTATG AAGACAAAAG TTTGTATGAC 
AAATTCACGA CCAGTTTTTC TGAGCATCAG ATGGAGGGCA ATGAGTCATC TCCTACAGAT 
GTACCAAGCC AGAAGGAGTC TGGAAAGCTG GACGTGAAGG AGCAGGAGGA GGGGCACCCC 
ATCGAATCAG AGGGCGTGAC CTTGACCACC ATCATGACTA TACCTCCCAA TGAGGAACAG 
ATGGAGTTCT CGCTGGGAGA GACAACAGAG GGGGACATAG GCCCCTTCAG CTCCATCAAG 
GTGCCCGTCA TCTTCACCCC GATTGTCCCG GGAACGGTCC AGAACAGTTT CAAGGTTGTG 
TTTAAGAATC AGCAGTGCCC AACTTTATAT TTCAAAGTCG TTGGCGTGGC TATCGATGTG 
CCTGTCTGGG TACCAAAGCC CAACGTAGAC CTGAAGATCT GCATGTACGA TCGGCTGTAC 
CAGGACTCGA TCCTCGTCCA CACACGGTCG AAGGCCGCGT TGCGCCTAAA GTTCGAGGTG 
TGCAAGGAGC TGCGGGGCCA CATGGAGCTC CTGCCTGAGA CTGGCTACAT CCAGGCCCTG 
TCGTCCTACT CAGTGCAGCT CAAGTTCCTG CCCAGACACT CCCTCCCAGA AGACGCAGGG 
AAGTATTTTG ACAAGGAGAC CAGGGTTCTG GAGGCCCCGA TGACTATCCG GGTGGCTGAC 
CAGATCAAAC CCGTGGGGTT TACTGTCCAT GCTATAGTCA CCACCTCGGA CCTGGAGCTC 
AGCCCCGCAG GGCTGGACTT TGGCTACTGT ACCATCTACG AGGCCATCAG GACCCAAATC 
AGCCTCTGCA ATCATTCCCT CCTGCCCCAG GAGTTTGGGT TCGTCGGACT CCCCAAGTTT 
GTGGACATCC AGCCCAACGA CGGGTTCGGG ACAATCCTGC CACTGGAAAC GCTACAGCTC 
GATGTGATCT TTCAGCCCAC CAAGGCCAAG GAGTACAACT TTGAGCTCAT CTGCAAGTCA 
GAGATTAATC GGTGCTTCAA GCTGGCATGC CGGGCCGTGG GTGTCTACCC GCCCCTGGAG 
CTGTCCCACA ACCAGATCAA GTTTGCAGCC ACCTCCTTGT ACGACACATC CGTGGCCACC 
CTGTATGTCA TCAACTCCCA CTTGAGCATG AACTCACTGA CCCACTCAGT GCCCCGCATT 
GGCTCGGAGG ATGCTTTTCC CGTGGGGCCC ACGTCTTTTG AGTTCCTGCT GCCCCCAGAT 
TCGCCCATCA CCATCTCACC CCTGGTGGGC ACCGTGTGGC CAGGAAAGAG GTGCCTGGTC 
CGAGTGGCCT TCCGGCCAGT GCTGCCCAGC GAGCTGATCC ACCAGGAGGC CTTCCAGGCC 
CTGAGCAGAA AAGCTGAGGT CAAATCATCC CAAAAGGAGA CGGTCACCCA GAGGAAGGAC 
CAGCGGAGAC CATCTTTCTC CATGCTGCGG CTGCAGAACC AGGACCAGCT GCTCCGGGCC 
TCAGCCTCCC AGCCTCCGGA GCTGCAGAAG GAGGACCTCG CATCCAGCTC TCAAGAGTAC 
CAGTCGGCAC AGGCCTCCCT GACCAGGTCT TTCCAGGGGA AGTTTGACAA GTTTGTGGTT 
CCCTGTGTTG TTGCTAGTGG CGACACCAAA GACAGGAAGG GGACAGAACC GCTGAGCTTC 
AGTCCCCACA ACACGCTGTA CCTGGAGCTG TGGTGTCCAG CGGTGGCGCC GTCTATCGTG 
GTGACGTCTG ACAAAGGCAA GACCATCATT AACTTCGGCG ACATTGCTGC AGGACACCGG 
GGCATAAAGA AGGTCTCCAT CCAGAACATC TCCCCTGAGG ATCTCGACCT GGAGTACTCC 
GTGCTGAACC CCGATGGCCC CTTCGTCCTG CTGAACCCCA CCATCAGGTT GCCCTCCGGC 
GAGACCCAGG TCCTAGATCT GTCCTTCTCC CCACGTGAGA GTGTCGTGGC CCAAGAAACA 
CTGGACATCA TCACCAAGAA AGGCACCCTC TCCCTGGCCC TCATGGGCAC GGGTGTGGCG 
TCCACGATCA CGTGCTCCAT TGAAGGTGAT GTCCTCAACA TGGGCTACGT GATTGCCAGA 
GAGTCTGTCT CCTTGGGCTT CAAGCTGCAG AACAACTCCT CACTGCCCAT CAAGTTCTCC 
ATGCAGCTGG ACAGCCTCTC CTCCAGGAGC AGAGACCAGC ACCGCCTGCC ACAGTTCCTG 
GCCTCGCCTG ACCAGAGGAC GGAAGTCGTG GGCACGCAGA ACTACAGTGG CCAGAGCGTG 
TTCAGCGTGG TCCCGGTCGA GGGTGTCATG GAGCCTGGCA AGGCTCAGGA CTTCACTGTG 
ACCTTCAGCC CAGACCACGA GAGCCTGTAC TTCTCTGACC GGCTGCAGGT CGTGCTCTTT 
GAAAAGAAAG TGTCCCACCA CATCCTCCTG AAGGGCGCAG CCCGCGAGCA CATGATGTTC 
GTGGAGGGCG GAGACCCCCT GGACGTGCCC GTGGAGTCCC TGACCGTGAT CCCTGCCTTT 
GACCTGGAGC ACAGAGAGGG CCCTCCCTGT CCAGAAGCTG AGGAGCTAAA GCCCATCCTG 
GTGACCCTGG ACTACGCCCA GCTGGATCCA GAAACACAGG CCCCACCTGC CACCCGAGAA 
CTGCAGGTGG GCTGCATCCG GACTACCCAG CTGTCCACAA AGAAGACTGT TGAGTTCAGC 
ATGGACAACA TCGCAGCCCT GCAGCACAAG GGCTTCACCA TTGAGCCCTC CAGAGGCTCT 
GTGGAGCGCG GCCAGACCAA GGCCATCAGC ATCTCCTGGA CGCCACCCAT CGACTTTGAT 
CCAGACCACC CACTCTTGAC ATCGGCCCTG CTGCAGCTCA GGGGGGATGT GAAGGAGACC 
TACAAGGTCC TCTTTGTAGC CCAGGTGGTG ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141547.1
CDS	110..4291
Translation	MWEGIRKGSPGGFSGEAAGSAWGVVMTSSPPVSLETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_025285762.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285762.1

ORF Insert Sequence:

ATGTGGGAAG GGATCCGCAA GGGAAGCCCT GGAGGATTTT CAGGAGAGGC AGCTGGGAGT 
GCATGGGGAG TGGTGATGAC GTCCTCACCA CCTGTGTCTC TGGAAACGCT CCGGAAATTT 
CAAGCATGGG GCCAAGCCAA GACAGAGTTG GCAGAACAGA AGGCCCAGGC CGAGAAGAAG 
GCAATTCTGG CCCAAGGTGG GGACGCCTTT AAGCACCTGT GCCATCAGCG CCGGCGCCAG 
GAGTTGGAGG CCCAGAACCG CGCCTTCGAG GAGGAGCAGA AGCGCAGGAA GCAGGAGATT 
GTGAGTCGGC TCTTGAAGGA AGAGGCCATG GAGGACCGGA GGAGGGCACG GCCCGAGCCC 
CCCAGACCCG CAGGCCAGCG GAGCCTGAGG GACCAGACCT GGAGCTACGT GGCCACCTTC 
TGCGATGGGC AGAGCACAGA GGCCCCGCTC TGCTGTCCCC TGCCTGATGT CCCTGGACAG 
AGGCCAGGTC CAGCCCAAGA GCAGATAAAG CTCCAGGAGA CCAGGTCCAG ATTCTGCCAG 
TCCTCTCCAT TGCTGACGGC CATCTCCAGT GAGTCTGTCC AGGGGGACGG GAGCCTCGGC 
GGGGAGGACA CACTGGCTTG GCCGGAGATC CCCGGGCTCT GGAATGAGGA CTACAAGCCT 
TTCCAGGTAT CCAAGGAGGA TGTGGACCAG AAGCCGGTGG GTGGAACAAA GATGGACAAG 
GACATCCTGG CGCGGACAAT GCAACGACTA CGCAGTGGGG TGATCCACAA ACAGGTGGCA 
TCAGGGCGTG AGTTCAGAGG GTGCCCCTTC AACAGCAAGC CCAAGTTCAT CCACTTCAAG 
GACTTTGATG TGGGCAAAGT GTACAAGAAG AAGATCACGC TGATCAATGC CACCTACACG 
ATTAACTACT GCAAGCTGGT GGGCGTGGAC GAGAGCCTGC AGGACTTCAT CCACGTGGAC 
TTTGACCCCC CGGGCCCACT ATCAGCCGGC ATGTCCTGCG AAGTGCTTGT CACCTTCAAG 
CCCATGATAA ACAAAGATCT GGAAGGAAGT GTCTCGTTTC TGGCTCAGAC AGGCGGATTT 
TCTGTTCCAC TGAAATGTTC AACAAAGAAA TGCTCTCTGT CTCTCGACAA GGAGCTCATC 
GACTTTGGCA GCTACACAGT GGGTGAGACT GCAATCCGGA CCATCACGCT GACCAACACT 
GGGGGCTTGG GCACCAAGTT CAGATTTCTT CTGGCATCAG AGACTTGTGA GATGGACACC 
TCCCAGTCGG TCCTAAAATT AAGTAGTACC TTCACGTATG AAGACAAAAG TTTGTATGAC 
AAATTCACGA CCAGTTTTTC TGAGCATCAG ATGGAGGGCA ATGAGTCATC TCCTACAGAT 
GTACCAAGCC AGAAGGAGTC TGGAAAGCTG GACGTGAAGG AGCAGGAGGA GGGGCACCCC 
ATCGAATCAG AGGGCGTGAC CTTGACCACC ATCATGACTA TACCTCCCAA TGAGGAACAG 
ATGGAGTTCT CGCTGGGAGA GACAACAGAG GGGGACATAG GCCCCTTCAG CTCCATCAAG 
GTGCCCGTCA TCTTCACCCC GATTGTCCCG GGAACGGTCC AGAACAGTTT CAAGGTTGTG 
TTTAAGAATC AGCAGTGCCC AACTTTATAT TTCAAAGTCG TTGGCGTGGC TATCGATGTG 
CCTGTCTGGG TACCAAAGCC CAACGTAGAC CTGAAGATCT GCATGTACGA TCGGCTGTAC 
CAGGACTCGA TCCTCGTCCA CACACGGTCG AAGGCCGCGT TGCGCCTAAA GTTCGAGGTG 
TGCAAGGAGC TGCGGGGCCA CATGGAGCTC CTGCCTGAGA CTGGCTACAT CCAGGCCCTG 
TCGTCCTACT CAGTGCAGCT CAAGTTCCTG CCCAGACACT CCCTCCCAGA AGACGCAGGG 
AAGTATTTTG ACAAGGAGAC CAGGGTTCTG GAGGCCCCGA TGACTATCCG GGTGGCTGAC 
CAGATCAAAC CCGTGGGGTT TACTGTCCAT GCTATAGTCA CCACCTCGGA CCTGGAGCTC 
AGCCCCGCAG GGCTGGACTT TGGCTACTGT ACCATCTACG AGGCCATCAG GACCCAAATC 
AGCCTCTGCA ATCATTCCCT CCTGCCCCAG GAGTTTGGGT TCGTCGGACT CCCCAAGTTT 
GTGGACATCC AGCCCAACGA CGGGTTCGGG ACAATCCTGC CACTGGAAAC GCTACAGCTC 
GATGTGATCT TTCAGCCCAC CAAGGCCAAG GAGTACAACT TTGAGCTCAT CTGCAAGTCA 
GAGATTAATC GGTGCTTCAA GCTGGCATGC CGGGCCGTGG GTGTCTACCC GCCCCTGGAG 
CTGTCCCACA ACCAGATCAA GTTTGCAGCC ACCTCCTTGT ACGACACATC CGTGGCCACC 
CTGTATGTCA TCAACTCCCA CTTGAGCATG AACTCACTGA CCCACTCAGT GCCCCGCATT 
GGCTCGGAGG ATGCTTTTCC CGTGGGGCCC ACGTCTTTTG AGTTCCTGCT GCCCCCAGAT 
TCGCCCATCA CCATCTCACC CCTGGTGGGC ACCGTGTGGC CAGGAAAGAG GTGCCTGGTC 
CGAGTGGCCT TCCGGCCAGT GCTGCCCAGC GAGCTGATCC ACCAGGAGGC CTTCCAGGCC 
CTGAGCAGAA AAGCTGAGGT CAAATCATCC CAAAAGGAGA CGGTCACCCA GAGGAAGGAC 
CAGCGGAGAC CATCTTTCTC CATGCTGCGG CTGCAGAACC AGGACCAGCT GCTCCGGGCC 
TCAGCCTCCC AGCCTCCGGA GCTGCAGAAG GAGGACCTCG CATCCAGCTC TCAAGAGTAC 
CAGTCGGCAC AGGCCTCCCT GACCAGGTCT TTCCAGGGGA AGTTTGACAA GTTTGTGGTT 
CCCTGTGTTG TTGCTAGTGG CGACACCAAA GACAGGAAGG GGACAGAACC GCTGAGCTTC 
AGTCCCCACA ACACGCTGTA CCTGGAGCTG TGGTGTCCAG CGGTGGCGCC GTCTATCGTG 
GTGACGTCTG ACAAAGGCAA GACCATCATT AACTTCGGCG ACATTGCTGC AGGACACCGG 
GGCATAAAGA AGGTCTCCAT CCAGAACATC TCCCCTGAGG ATCTCGACCT GGAGTACTCC 
GTGCTGAACC CCGATGGCCC CTTCGTCCTG CTGAACCCCA CCATCAGGTT GCCCTCCGGC 
GAGACCCAGG TCCTAGATCT GTCCTTCTCC CCACGTGAGA GTGTCGTGGC CCAAGAAACA 
CTGGACATCA TCACCAAGAA AGGCACCCTC TCCCTGGCCC TCATGGGCAC GGGTGTGGCG 
TCCACGATCA CGTGCTCCAT TGAAGGTGAT GTCCTCAACA TGGGCTACGT GATTGCCAGA 
GAGTCTGTCT CCTTGGGCTT CAAGCTGCAG AACAACTCCT CACTGCCCAT CAAGTTCTCC 
ATGCAGCTGG ACAGCCTCTC CTCCAGGAGC AGAGACCAGC ACCGCCTGCC ACAGTTCCTG 
GCCTCGCCTG ACCAGAGGAC GGAAGTCGTG GGCACGCAGA ACTACAGTGG CCAGAGCGTG 
TTCAGCGTGG TCCCGGTCGA GGGTGTCATG GAGCCTGGCA AGGCTCAGGA CTTCACTGTG 
ACCTTCAGCC CAGACCACGA GAGCCTGTAC TTCTCTGACC GGCTGCAGGT CGTGCTCTTT 
GAAAAGAAAG TGTCCCACCA CATCCTCCTG AAGGGCGCAG CCCGCGAGCA CATGATGTTC 
GTGGAGGGCG GAGACCCCCT GGACGTGCCC GTGGAGTCCC TGACCGTGAT CCCTGCCTTT 
GACCTGGAGC ACAGAGAGGG CCCTCCCTGT CCAGAAGCTG AGGAGCTAAA GCCCATCCTG 
GTGACCCTGG ACTACGCCCA GCTGGATCCA GAAACACAGG CCCCACCTGC CACCCGAGAA 
CTGCAGGTGG GCTGCATCCG GACTACCCAG CTGTCCACAA AGAAGACTGT TGAGTTCAGC 
ATGGACAACA TCGCAGCCCT GCAGCACAAG GGCTTCACCA TTGAGCCCTC CAGAGGCTCT 
GTGGAGCGCG GCCAGACCAA GGCCATCAGC ATCTCCTGGA CGCCACCCAT CGACTTTGAT 
CCAGACCACC CACTCTTGAC ATCGGCCCTG CTGCAGCTCA GGGGGGATGT GAAGGAGACC 
TACAAGGTCC TCTTTGTAGC CCAGGTGGTG ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044662.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044724.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X1, mRNA.

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CloneID	OBu03800
Clone ID Related Accession (Same CDS sequence)	XM_006044662.1
Accession Version	XM_006044662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044724.1
CDS	115..5064
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044662.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044662.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044663.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044725.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X2, mRNA.

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CloneID	OBu03801
Clone ID Related Accession (Same CDS sequence)	XM_006044663.1
Accession Version	XM_006044663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGTCCC AAAAGGAGAC GGTCACCCAG AGGAAGGACC AGCGGAGACC ATCTTTCTCC 
ATGCTGCGGC TGCAGAACCA GGACCAGCTG CTCCGGGCCT CAGCCTCCCA GCCTCCGGAG 
CTGCAGAAGG AGGACCTCGC ATCCAGCTCT CAAGAGTACC AGTCGGCACA GGCCTCCCTG 
ACCAGGTCTT TCCAGGGGAA GTTTGACAAG TTTGTGGTTC CCTGTGTTGT TGCTAGTGGC 
GACACCAAAG ACAGGAAGGG GACAGAACCG CTGAGCTTCA GTCCCCACAA CACGCTGTAC 
CTGGAGCTGT GGTGTCCAGC GGTGGCGCCG TCTATCGTGG TGACGTCTGA CAAAGGCAAG 
ACCATCATTA ACTTCGGCGA CATTGCTGCA GGACACCGGG GCATAAAGAA GGTCTCCATC 
CAGAACATCT CCCCTGAGGA TCTCGACCTG GAGTACTCCG TGCTGAACCC CGATGGCCCC 
TTCGTCCTGC TGAACCCCAC CATCAGGTTG CCCTCCGGCG AGACCCAGGT CCTAGATCTG 
TCCTTCTCCC CACGTGAGAG TGTCGTGGCC CAAGAAACAC TGGACATCAT CACCAAGAAA 
GGCACCCTCT CCCTGGCCCT CATGGGCACG GGTGTGGCGT CCACGATCAC GTGCTCCATT 
GAAGGTGATG TCCTCAACAT GGGCTACGTG ATTGCCAGAG AGTCTGTCTC CTTGGGCTTC 
AAGCTGCAGA ACAACTCCTC ACTGCCCATC AAGTTCTCCA TGCAGCTGGA CAGCCTCTCC 
TCCAGGAGCA GAGACCAGCA CCGCCTGCCA CAGTTCCTGG CCTCGCCTGA CCAGAGGACG 
GAAGTCGTGG GCACGCAGAA CTACAGTGGC CAGAGCGTGT TCAGCGTGGT CCCGGTCGAG 
GGTGTCATGG AGCCCGGCAA GGCTCAGGAC TTCACTGTGA CCTTCAGCCC AGACCACGAG 
AGCCTGTACT TCTCTGACCG GCTGCAGGTC GTGCTCTTTG AAAAGAAAGT GTCCCACCAC 
ATCCTCCTGA AGGGCGCAGC CCGCGAGCAC ATGATGTTCG TGGAGGGCGG AGACCCCCTG 
GACGTGCCCG TGGAGTCCCT GACCGTGATC CCTGCCTTTG ACCTGGAGCA CAGAGAGGGC 
CCTCCCTGTC CAGAAGCTGA GGAGCTAAAG CCCATCCTGG TGACCCTGGA CTACGCCCAG 
CTGGATCCAG AAACACAGGC CCCACCTGCC ACCCGAGAAC TGCAGGTGGG CTGCATCCGG 
ACTACCCAGC TGTCCACAAA GAAGACTGTT GAGTTCAGCA TGGACAACAT CGCAGCCCTG 
CAGCACAAGG GCTTCACCAT TGAGCCCTCC AGAGGCTCTG TGGAGCGCGG CCAGACCAAG 
GCCATCAGCA TCTCCTGGAC GCCACCCATC GACTTTGATC CAGACCACCC ACTCTTGACA 
TCGGCCCTGC TGCAGCTCAG GGGGGATGTG AAGGAGACCT ACAAGGTCCT CTTTGTAGCC 
CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044725.1
CDS	115..5055
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAESQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044663.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044663.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGTCCC AAAAGGAGAC GGTCACCCAG AGGAAGGACC AGCGGAGACC ATCTTTCTCC 
ATGCTGCGGC TGCAGAACCA GGACCAGCTG CTCCGGGCCT CAGCCTCCCA GCCTCCGGAG 
CTGCAGAAGG AGGACCTCGC ATCCAGCTCT CAAGAGTACC AGTCGGCACA GGCCTCCCTG 
ACCAGGTCTT TCCAGGGGAA GTTTGACAAG TTTGTGGTTC CCTGTGTTGT TGCTAGTGGC 
GACACCAAAG ACAGGAAGGG GACAGAACCG CTGAGCTTCA GTCCCCACAA CACGCTGTAC 
CTGGAGCTGT GGTGTCCAGC GGTGGCGCCG TCTATCGTGG TGACGTCTGA CAAAGGCAAG 
ACCATCATTA ACTTCGGCGA CATTGCTGCA GGACACCGGG GCATAAAGAA GGTCTCCATC 
CAGAACATCT CCCCTGAGGA TCTCGACCTG GAGTACTCCG TGCTGAACCC CGATGGCCCC 
TTCGTCCTGC TGAACCCCAC CATCAGGTTG CCCTCCGGCG AGACCCAGGT CCTAGATCTG 
TCCTTCTCCC CACGTGAGAG TGTCGTGGCC CAAGAAACAC TGGACATCAT CACCAAGAAA 
GGCACCCTCT CCCTGGCCCT CATGGGCACG GGTGTGGCGT CCACGATCAC GTGCTCCATT 
GAAGGTGATG TCCTCAACAT GGGCTACGTG ATTGCCAGAG AGTCTGTCTC CTTGGGCTTC 
AAGCTGCAGA ACAACTCCTC ACTGCCCATC AAGTTCTCCA TGCAGCTGGA CAGCCTCTCC 
TCCAGGAGCA GAGACCAGCA CCGCCTGCCA CAGTTCCTGG CCTCGCCTGA CCAGAGGACG 
GAAGTCGTGG GCACGCAGAA CTACAGTGGC CAGAGCGTGT TCAGCGTGGT CCCGGTCGAG 
GGTGTCATGG AGCCCGGCAA GGCTCAGGAC TTCACTGTGA CCTTCAGCCC AGACCACGAG 
AGCCTGTACT TCTCTGACCG GCTGCAGGTC GTGCTCTTTG AAAAGAAAGT GTCCCACCAC 
ATCCTCCTGA AGGGCGCAGC CCGCGAGCAC ATGATGTTCG TGGAGGGCGG AGACCCCCTG 
GACGTGCCCG TGGAGTCCCT GACCGTGATC CCTGCCTTTG ACCTGGAGCA CAGAGAGGGC 
CCTCCCTGTC CAGAAGCTGA GGAGCTAAAG CCCATCCTGG TGACCCTGGA CTACGCCCAG 
CTGGATCCAG AAACACAGGC CCCACCTGCC ACCCGAGAAC TGCAGGTGGG CTGCATCCGG 
ACTACCCAGC TGTCCACAAA GAAGACTGTT GAGTTCAGCA TGGACAACAT CGCAGCCCTG 
CAGCACAAGG GCTTCACCAT TGAGCCCTCC AGAGGCTCTG TGGAGCGCGG CCAGACCAAG 
GCCATCAGCA TCTCCTGGAC GCCACCCATC GACTTTGATC CAGACCACCC ACTCTTGACA 
TCGGCCCTGC TGCAGCTCAG GGGGGATGTG AAGGAGACCT ACAAGGTCCT CTTTGTAGCC 
CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044664.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044726.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X3, mRNA.

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CloneID	OBu03802
Clone ID Related Accession (Same CDS sequence)	XM_006044664.1
Accession Version	XM_006044664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAT 
CCAGAAACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCA CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACGCCACC CATCGACTTT GATCCAGACC ACCCACTCTT GACATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044726.1
CDS	115..5049
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044664.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044664.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAT 
CCAGAAACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCA CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACGCCACC CATCGACTTT GATCCAGACC ACCCACTCTT GACATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044665.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044727.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu03803
Clone ID Related Accession (Same CDS sequence)	XM_006044665.1
Accession Version	XM_006044665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCAG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGG CCCTCCCTGT 
CCAGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGATCCA 
GAAACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCACAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CGCCACCCAT CGACTTTGAT CCAGACCACC CACTCTTGAC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044727.1
CDS	116..5047
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044665.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044665.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCCGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCAG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGG CCCTCCCTGT 
CCAGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGATCCA 
GAAACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCACAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CGCCACCCAT CGACTTTGAT CCAGACCACC CACTCTTGAC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044666.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044728.1 Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X5, mRNA.

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CloneID	OBu03804
Clone ID Related Accession (Same CDS sequence)	XM_006044666.1
Accession Version	XM_006044666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	uncharacterized protein KIAA1751 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783645.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGGGG CACAGGACCG 
TCCAGATTCT GCCAGTCCTC TCCATTGCTG ACGGCCATCT CCAGTGAGTC TGTCCAGGGG 
GACGGGAGCC TCGGCGGGGA GGACACACTG GCTTGGCCGG AGATCCCCGG GCTCTGGAAT 
GAGGACTACA AGCCTTTCCA GGTATCCAAG GAGGATGTGG ACCAGAAGCC GGTGGGTGGA 
ACAAAGATGG ACAAGGACAT CCTGGCGCGG ACAATGCAAC GACTACGCAG TGGGGTGATC 
CACAAACAGG TGGCATCAGG GCGTGAGTTC AGAGGGTGCC CCTTCAACAG CAAGCCCAAG 
TTCATCCACT TCAAGGACTT TGATGTGGGC AAAGTGTACA AGAAGAAGAT CACGCTGATC 
AATGCCACCT ACACGATTAA CTACTGCAAG CTGGTGGGCG TGGACGAGAG CCTGCAGGAC 
TTCATCCACG TGGACTTTGA CCCCCCGGGC CCACTATCAG CCGGCATGTC CTGCGAAGTG 
CTTGTCACCT TCAAGCCCAT GATAAACAAA GATCTGGAAG GAAGTGTCTC GTTTCTGGCT 
CAGACAGGCG GATTTTCTGT TCCACTGAAA TGTTCAACAA AGAAATGCTC TCTGTCTCTC 
GACAAGGAGC TCATCGACTT TGGCAGCTAC ACAGTGGGTG AGACTGCAAT CCGGACCATC 
ACGCTGACCA ACACTGGGGG CTTGGGCACC AAGTTCAGAT TTCTTCTGGC ATCAGAGACT 
TGTGAGATGG ACACCTCCCA GTCGGTCCTA AAATTAAGTA GTACCTTCAC GTATGAAGAC 
AAAAGTTTGT ATGACAAATT CACGACCAGT TTTTCTGAGC ATCAGATGGA GGGCAATGAG 
TCATCTCCTA CAGATGTACC AAGCCAGAAG GAGTCTGGAA AGCTGGACGT GAAGGAGCAG 
GAGGAGGGGC ACCCCATCGA ATCAGAGGGC GTGACCTTGA CCACCATCAT GACTATACCT 
CCCAATGAGG AACAGATGGA GTTCTCGCTG GGAGAGACAA CAGAGGGGGA CATAGGCCCC 
TTCAGCTCCA TCAAGGTGCC CGTCATCTTC ACCCCGATTG TCCCGGGAAC GGTCCAGAAC 
AGTTTCAAGG TTGTGTTTAA GAATCAGCAG TGCCCAACTT TATATTTCAA AGTCGTTGGC 
GTGGCTATCG ATGTGCCTGT CTGGGTACCA AAGCCCAACG TAGACCTGAA GATCTGCATG 
TACGATCGGC TGTACCAGGA CTCGATCCTC GTCCACACAC GGTCGAAGGC CGCGTTGCGC 
CTAAAGTTCG AGGTGTGCAA GGAGCTGCGG GGCCACATGG AGCTCCTGCC TGAGACTGGC 
TACATCCAGG CCCTGTCGTC CTACTCAGTG CAGCTCAAGT TCCTGCCCAG ACACTCCCTC 
CCAGAAGACG CAGGGAAGTA TTTTGACAAG GAGACCAGGG TTCTGGAGGC CCCGATGACT 
ATCCGGGTGG CTGACCAGAT CAAACCCGTG GGGTTTACTG TCCATGCTAT AGTCACCACC 
TCGGACCTGG AGCTCAGCCC CGCAGGGCTG GACTTTGGCT ACTGTACCAT CTACGAGGCC 
ATCAGGACCC AAATCAGCCT CTGCAATCAT TCCCTCCTGC CCCAGGAGTT TGGGTTCGTC 
GGACTCCCCA AGTTTGTGGA CATCCAGCCC AACGACGGGT TCGGGACAAT CCTGCCACTG 
GAAACGCTAC AGCTCGATGT GATCTTTCAG CCCACCAAGG CCAAGGAGTA CAACTTTGAG 
CTCATCTGCA AGTCAGAGAT TAATCGGTGC TTCAAGCTGG CATGCCGGGC CGTGGGTGTC 
TACCCGCCCC TGGAGCTGTC CCACAACCAG ATCAAGTTTG CAGCCACCTC CTTGTACGAC 
ACATCCGTGG CCACCCTGTA TGTCATCAAC TCCCACTTGA GCATGAACTC ACTGACCCAC 
TCAGTGCCCC GCATTGGCTC GGAGGATGCT TTTCCCGTGG GGCCCACGTC TTTTGAGTTC 
CTGCTGCCCC CAGATTCGCC CATCACCATC TCACCCCTGG TGGGCACCGT GTGGCCAGGA 
AAGAGGTGCC TGGTCCGAGT GGCCTTCCGG CCAGTGCTGC CCAGCGAGCT GATCCACCAG 
GAGGCCTTCC AGGCCCTGAG CAGAAAAGCT GAGGTCAAAT CATCCCAAAA GGAGACGGTC 
ACCCAGAGGA AGGACCAGCG GAGACCATCT TTCTCCATGC TGCGGCTGCA GAACCAGGAC 
CAGCTGCTCC GGGCCTCAGC CTCCCAGCCT CCGGAGCTGC AGAAGGAGGA CCTCGCATCC 
AGCTCTCAAG AGTACCAGTC GGCACAGGCC TCCCTGACCA GGTCTTTCCA GGGGAAGTTT 
GACAAGTTTG TGGTTCCCTG TGTTGTTGCT AGTGGCGACA CCAAAGACAG GAAGGGGACA 
GAACCGCTGA GCTTCAGTCC CCACAACACG CTGTACCTGG AGCTGTGGTG TCCAGCGGTG 
GCGCCGTCTA TCGTGGTGAC GTCTGACAAA GGCAAGACCA TCATTAACTT CGGCGACATT 
GCTGCAGGAC ACCGGGGCAT AAAGAAGGTC TCCATCCAGA ACATCTCCCC TGAGGATCTC 
GACCTGGAGT ACTCCGTGCT GAACCCCGAT GGCCCCTTCG TCCTGCTGAA CCCCACCATC 
AGGTTGCCCT CCGGCGAGAC CCAGGTCCTA GATCTGTCCT TCTCCCCACG TGAGAGTGTC 
GTGGCCCAAG AAACACTGGA CATCATCACC AAGAAAGGCA CCCTCTCCCT GGCCCTCATG 
GGCACGGGTG TGGCGTCCAC GATCACGTGC TCCATTGAAG GTGATGTCCT CAACATGGGC 
TACGTGATTG CCAGAGAGTC TGTCTCCTTG GGCTTCAAGC TGCAGAACAA CTCCTCACTG 
CCCATCAAGT TCTCCATGCA GCTGGACAGC CTCTCCTCCA GGAGCAGAGA CCAGCACCGC 
CTGCCACAGT TCCTGGCCTC GCCTGACCAG AGGACGGAAG TCGTGGGCAC GCAGAACTAC 
AGTGGCCAGA GCGTGTTCAG CGTGGTCCCG GTCGAGGGTG TCATGGAGCC CGGCAAGGCT 
CAGGACTTCA CTGTGACCTT CAGCCCAGAC CACGAGAGCC TGTACTTCTC TGACCGGCTG 
CAGGTCGTGC TCTTTGAAAA GAAAGTGTCC CACCACATCC TCCTGAAGGG CGCAGCCCGC 
GAGCACATGA TGTTCGTGGA GGGCGGAGAC CCCCTGGACG TGCCCGTGGA GTCCCTGACC 
GTGATCCCTG CCTTTGACCT GGAGCACAGA GAGGGCCCTC CCTGTCCAGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ATCCAGAAAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CACAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACGCCA 
CCCATCGACT TTGATCCAGA CCACCCACTC TTGACATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044728.1
CDS	116..5032
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLVSGCPGGTGPSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044666.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1751 ortholog (KIAA1751), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044666.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG GTGAGCGGAT GCCCTGGGGG CACAGGACCG 
TCCAGATTCT GCCAGTCCTC TCCATTGCTG ACGGCCATCT CCAGTGAGTC TGTCCAGGGG 
GACGGGAGCC TCGGCGGGGA GGACACACTG GCTTGGCCGG AGATCCCCGG GCTCTGGAAT 
GAGGACTACA AGCCTTTCCA GGTATCCAAG GAGGATGTGG ACCAGAAGCC GGTGGGTGGA 
ACAAAGATGG ACAAGGACAT CCTGGCGCGG ACAATGCAAC GACTACGCAG TGGGGTGATC 
CACAAACAGG TGGCATCAGG GCGTGAGTTC AGAGGGTGCC CCTTCAACAG CAAGCCCAAG 
TTCATCCACT TCAAGGACTT TGATGTGGGC AAAGTGTACA AGAAGAAGAT CACGCTGATC 
AATGCCACCT ACACGATTAA CTACTGCAAG CTGGTGGGCG TGGACGAGAG CCTGCAGGAC 
TTCATCCACG TGGACTTTGA CCCCCCGGGC CCACTATCAG CCGGCATGTC CTGCGAAGTG 
CTTGTCACCT TCAAGCCCAT GATAAACAAA GATCTGGAAG GAAGTGTCTC GTTTCTGGCT 
CAGACAGGCG GATTTTCTGT TCCACTGAAA TGTTCAACAA AGAAATGCTC TCTGTCTCTC 
GACAAGGAGC TCATCGACTT TGGCAGCTAC ACAGTGGGTG AGACTGCAAT CCGGACCATC 
ACGCTGACCA ACACTGGGGG CTTGGGCACC AAGTTCAGAT TTCTTCTGGC ATCAGAGACT 
TGTGAGATGG ACACCTCCCA GTCGGTCCTA AAATTAAGTA GTACCTTCAC GTATGAAGAC 
AAAAGTTTGT ATGACAAATT CACGACCAGT TTTTCTGAGC ATCAGATGGA GGGCAATGAG 
TCATCTCCTA CAGATGTACC AAGCCAGAAG GAGTCTGGAA AGCTGGACGT GAAGGAGCAG 
GAGGAGGGGC ACCCCATCGA ATCAGAGGGC GTGACCTTGA CCACCATCAT GACTATACCT 
CCCAATGAGG AACAGATGGA GTTCTCGCTG GGAGAGACAA CAGAGGGGGA CATAGGCCCC 
TTCAGCTCCA TCAAGGTGCC CGTCATCTTC ACCCCGATTG TCCCGGGAAC GGTCCAGAAC 
AGTTTCAAGG TTGTGTTTAA GAATCAGCAG TGCCCAACTT TATATTTCAA AGTCGTTGGC 
GTGGCTATCG ATGTGCCTGT CTGGGTACCA AAGCCCAACG TAGACCTGAA GATCTGCATG 
TACGATCGGC TGTACCAGGA CTCGATCCTC GTCCACACAC GGTCGAAGGC CGCGTTGCGC 
CTAAAGTTCG AGGTGTGCAA GGAGCTGCGG GGCCACATGG AGCTCCTGCC TGAGACTGGC 
TACATCCAGG CCCTGTCGTC CTACTCAGTG CAGCTCAAGT TCCTGCCCAG ACACTCCCTC 
CCAGAAGACG CAGGGAAGTA TTTTGACAAG GAGACCAGGG TTCTGGAGGC CCCGATGACT 
ATCCGGGTGG CTGACCAGAT CAAACCCGTG GGGTTTACTG TCCATGCTAT AGTCACCACC 
TCGGACCTGG AGCTCAGCCC CGCAGGGCTG GACTTTGGCT ACTGTACCAT CTACGAGGCC 
ATCAGGACCC AAATCAGCCT CTGCAATCAT TCCCTCCTGC CCCAGGAGTT TGGGTTCGTC 
GGACTCCCCA AGTTTGTGGA CATCCAGCCC AACGACGGGT TCGGGACAAT CCTGCCACTG 
GAAACGCTAC AGCTCGATGT GATCTTTCAG CCCACCAAGG CCAAGGAGTA CAACTTTGAG 
CTCATCTGCA AGTCAGAGAT TAATCGGTGC TTCAAGCTGG CATGCCGGGC CGTGGGTGTC 
TACCCGCCCC TGGAGCTGTC CCACAACCAG ATCAAGTTTG CAGCCACCTC CTTGTACGAC 
ACATCCGTGG CCACCCTGTA TGTCATCAAC TCCCACTTGA GCATGAACTC ACTGACCCAC 
TCAGTGCCCC GCATTGGCTC GGAGGATGCT TTTCCCGTGG GGCCCACGTC TTTTGAGTTC 
CTGCTGCCCC CAGATTCGCC CATCACCATC TCACCCCTGG TGGGCACCGT GTGGCCAGGA 
AAGAGGTGCC TGGTCCGAGT GGCCTTCCGG CCAGTGCTGC CCAGCGAGCT GATCCACCAG 
GAGGCCTTCC AGGCCCTGAG CAGAAAAGCT GAGGTCAAAT CATCCCAAAA GGAGACGGTC 
ACCCAGAGGA AGGACCAGCG GAGACCATCT TTCTCCATGC TGCGGCTGCA GAACCAGGAC 
CAGCTGCTCC GGGCCTCAGC CTCCCAGCCT CCGGAGCTGC AGAAGGAGGA CCTCGCATCC 
AGCTCTCAAG AGTACCAGTC GGCACAGGCC TCCCTGACCA GGTCTTTCCA GGGGAAGTTT 
GACAAGTTTG TGGTTCCCTG TGTTGTTGCT AGTGGCGACA CCAAAGACAG GAAGGGGACA 
GAACCGCTGA GCTTCAGTCC CCACAACACG CTGTACCTGG AGCTGTGGTG TCCAGCGGTG 
GCGCCGTCTA TCGTGGTGAC GTCTGACAAA GGCAAGACCA TCATTAACTT CGGCGACATT 
GCTGCAGGAC ACCGGGGCAT AAAGAAGGTC TCCATCCAGA ACATCTCCCC TGAGGATCTC 
GACCTGGAGT ACTCCGTGCT GAACCCCGAT GGCCCCTTCG TCCTGCTGAA CCCCACCATC 
AGGTTGCCCT CCGGCGAGAC CCAGGTCCTA GATCTGTCCT TCTCCCCACG TGAGAGTGTC 
GTGGCCCAAG AAACACTGGA CATCATCACC AAGAAAGGCA CCCTCTCCCT GGCCCTCATG 
GGCACGGGTG TGGCGTCCAC GATCACGTGC TCCATTGAAG GTGATGTCCT CAACATGGGC 
TACGTGATTG CCAGAGAGTC TGTCTCCTTG GGCTTCAAGC TGCAGAACAA CTCCTCACTG 
CCCATCAAGT TCTCCATGCA GCTGGACAGC CTCTCCTCCA GGAGCAGAGA CCAGCACCGC 
CTGCCACAGT TCCTGGCCTC GCCTGACCAG AGGACGGAAG TCGTGGGCAC GCAGAACTAC 
AGTGGCCAGA GCGTGTTCAG CGTGGTCCCG GTCGAGGGTG TCATGGAGCC CGGCAAGGCT 
CAGGACTTCA CTGTGACCTT CAGCCCAGAC CACGAGAGCC TGTACTTCTC TGACCGGCTG 
CAGGTCGTGC TCTTTGAAAA GAAAGTGTCC CACCACATCC TCCTGAAGGG CGCAGCCCGC 
GAGCACATGA TGTTCGTGGA GGGCGGAGAC CCCCTGGACG TGCCCGTGGA GTCCCTGACC 
GTGATCCCTG CCTTTGACCT GGAGCACAGA GAGGGCCCTC CCTGTCCAGA AGCTGAGGAG 
CTAAAGCCCA TCCTGGTGAC CCTGGACTAC GCCCAGCTGG ATCCAGAAAC ACAGGCCCCA 
CCTGCCACCC GAGAACTGCA GGTGGGCTGC ATCCGGACTA CCCAGCTGTC CACAAAGAAG 
ACTGTTGAGT TCAGCATGGA CAACATCGCA GCCCTGCAGC ACAAGGGCTT CACCATTGAG 
CCCTCCAGAG GCTCTGTGGA GCGCGGCCAG ACCAAGGCCA TCAGCATCTC CTGGACGCCA 
CCCATCGACT TTGATCCAGA CCACCCACTC TTGACATCGG CCCTGCTGCA GCTCAGGGGG 
GATGTGAAGG AGACCTACAA GGTCCTCTTT GTAGCCCAGG TGGTGACCGG TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044662.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044724.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

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CloneID	OBu66179
Clone ID Related Accession (Same CDS sequence)	XM_006044662.2 , XM_025285761.1
Accession Version	XM_006044662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044724.1
CDS	409..5358
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044662.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044662.2

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_025285761.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141546.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

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CloneID	OBu66179
Clone ID Related Accession (Same CDS sequence)	XM_006044662.2 , XM_025285761.1
Accession Version	XM_025285761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141546.1
CDS	430..5379
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_025285761.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285761.1

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGGCC CTCCCTGTCC AGAAGCTGAG GAGCTAAAGC CCATCCTGGT GACCCTGGAC 
TACGCCCAGC TGGATCCAGA AACACAGGCC CCACCTGCCA CCCGAGAACT GCAGGTGGGC 
TGCATCCGGA CTACCCAGCT GTCCACAAAG AAGACTGTTG AGTTCAGCAT GGACAACATC 
GCAGCCCTGC AGCACAAGGG CTTCACCATT GAGCCCTCCA GAGGCTCTGT GGAGCGCGGC 
CAGACCAAGG CCATCAGCAT CTCCTGGACG CCACCCATCG ACTTTGATCC AGACCACCCA 
CTCTTGACAT CGGCCCTGCT GCAGCTCAGG GGGGATGTGA AGGAGACCTA CAAGGTCCTC 
TTTGTAGCCC AGGTGGTGAC CGGTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044663.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044725.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.

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CloneID	OBu66180
Clone ID Related Accession (Same CDS sequence)	XM_006044663.2
Accession Version	XM_006044663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGTCCC AAAAGGAGAC GGTCACCCAG AGGAAGGACC AGCGGAGACC ATCTTTCTCC 
ATGCTGCGGC TGCAGAACCA GGACCAGCTG CTCCGGGCCT CAGCCTCCCA GCCTCCGGAG 
CTGCAGAAGG AGGACCTCGC ATCCAGCTCT CAAGAGTACC AGTCGGCACA GGCCTCCCTG 
ACCAGGTCTT TCCAGGGGAA GTTTGACAAG TTTGTGGTTC CCTGTGTTGT TGCTAGTGGC 
GACACCAAAG ACAGGAAGGG GACAGAACCG CTGAGCTTCA GTCCCCACAA CACGCTGTAC 
CTGGAGCTGT GGTGTCCAGC GGTGGCGCCG TCTATCGTGG TGACGTCTGA CAAAGGCAAG 
ACCATCATTA ACTTCGGCGA CATTGCTGCA GGACACCGGG GCATAAAGAA GGTCTCCATC 
CAGAACATCT CCCCTGAGGA TCTCGACCTG GAGTACTCCG TGCTGAACCC CGATGGCCCC 
TTCGTCCTGC TGAACCCCAC CATCAGGTTG CCCTCCGGCG AGACCCAGGT CCTAGATCTG 
TCCTTCTCCC CACGTGAGAG TGTCGTGGCC CAAGAAACAC TGGACATCAT CACCAAGAAA 
GGCACCCTCT CCCTGGCCCT CATGGGCACG GGTGTGGCGT CCACGATCAC GTGCTCCATT 
GAAGGTGATG TCCTCAACAT GGGCTACGTG ATTGCCAGAG AGTCTGTCTC CTTGGGCTTC 
AAGCTGCAGA ACAACTCCTC ACTGCCCATC AAGTTCTCCA TGCAGCTGGA CAGCCTCTCC 
TCCAGGAGCA GAGACCAGCA CCGCCTGCCA CAGTTCCTGG CCTCGCCTGA CCAGAGGACG 
GAAGTCGTGG GCACGCAGAA CTACAGTGGC CAGAGCGTGT TCAGCGTGGT CCCGGTCGAG 
GGTGTCATGG AGCCTGGCAA GGCTCAGGAC TTCACTGTGA CCTTCAGCCC AGACCACGAG 
AGCCTGTACT TCTCTGACCG GCTGCAGGTC GTGCTCTTTG AAAAGAAAGT GTCCCACCAC 
ATCCTCCTGA AGGGCGCAGC CCGCGAGCAC ATGATGTTCG TGGAGGGCGG AGACCCCCTG 
GACGTGCCCG TGGAGTCCCT GACCGTGATC CCTGCCTTTG ACCTGGAGCA CAGAGAGGGC 
CCTCCCTGTC CAGAAGCTGA GGAGCTAAAG CCCATCCTGG TGACCCTGGA CTACGCCCAG 
CTGGATCCAG AAACACAGGC CCCACCTGCC ACCCGAGAAC TGCAGGTGGG CTGCATCCGG 
ACTACCCAGC TGTCCACAAA GAAGACTGTT GAGTTCAGCA TGGACAACAT CGCAGCCCTG 
CAGCACAAGG GCTTCACCAT TGAGCCCTCC AGAGGCTCTG TGGAGCGCGG CCAGACCAAG 
GCCATCAGCA TCTCCTGGAC GCCACCCATC GACTTTGATC CAGACCACCC ACTCTTGACA 
TCGGCCCTGC TGCAGCTCAG GGGGGATGTG AAGGAGACCT ACAAGGTCCT CTTTGTAGCC 
CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044725.1
CDS	409..5349
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAESQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044663.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044663.2

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGTCCC AAAAGGAGAC GGTCACCCAG AGGAAGGACC AGCGGAGACC ATCTTTCTCC 
ATGCTGCGGC TGCAGAACCA GGACCAGCTG CTCCGGGCCT CAGCCTCCCA GCCTCCGGAG 
CTGCAGAAGG AGGACCTCGC ATCCAGCTCT CAAGAGTACC AGTCGGCACA GGCCTCCCTG 
ACCAGGTCTT TCCAGGGGAA GTTTGACAAG TTTGTGGTTC CCTGTGTTGT TGCTAGTGGC 
GACACCAAAG ACAGGAAGGG GACAGAACCG CTGAGCTTCA GTCCCCACAA CACGCTGTAC 
CTGGAGCTGT GGTGTCCAGC GGTGGCGCCG TCTATCGTGG TGACGTCTGA CAAAGGCAAG 
ACCATCATTA ACTTCGGCGA CATTGCTGCA GGACACCGGG GCATAAAGAA GGTCTCCATC 
CAGAACATCT CCCCTGAGGA TCTCGACCTG GAGTACTCCG TGCTGAACCC CGATGGCCCC 
TTCGTCCTGC TGAACCCCAC CATCAGGTTG CCCTCCGGCG AGACCCAGGT CCTAGATCTG 
TCCTTCTCCC CACGTGAGAG TGTCGTGGCC CAAGAAACAC TGGACATCAT CACCAAGAAA 
GGCACCCTCT CCCTGGCCCT CATGGGCACG GGTGTGGCGT CCACGATCAC GTGCTCCATT 
GAAGGTGATG TCCTCAACAT GGGCTACGTG ATTGCCAGAG AGTCTGTCTC CTTGGGCTTC 
AAGCTGCAGA ACAACTCCTC ACTGCCCATC AAGTTCTCCA TGCAGCTGGA CAGCCTCTCC 
TCCAGGAGCA GAGACCAGCA CCGCCTGCCA CAGTTCCTGG CCTCGCCTGA CCAGAGGACG 
GAAGTCGTGG GCACGCAGAA CTACAGTGGC CAGAGCGTGT TCAGCGTGGT CCCGGTCGAG 
GGTGTCATGG AGCCTGGCAA GGCTCAGGAC TTCACTGTGA CCTTCAGCCC AGACCACGAG 
AGCCTGTACT TCTCTGACCG GCTGCAGGTC GTGCTCTTTG AAAAGAAAGT GTCCCACCAC 
ATCCTCCTGA AGGGCGCAGC CCGCGAGCAC ATGATGTTCG TGGAGGGCGG AGACCCCCTG 
GACGTGCCCG TGGAGTCCCT GACCGTGATC CCTGCCTTTG ACCTGGAGCA CAGAGAGGGC 
CCTCCCTGTC CAGAAGCTGA GGAGCTAAAG CCCATCCTGG TGACCCTGGA CTACGCCCAG 
CTGGATCCAG AAACACAGGC CCCACCTGCC ACCCGAGAAC TGCAGGTGGG CTGCATCCGG 
ACTACCCAGC TGTCCACAAA GAAGACTGTT GAGTTCAGCA TGGACAACAT CGCAGCCCTG 
CAGCACAAGG GCTTCACCAT TGAGCCCTCC AGAGGCTCTG TGGAGCGCGG CCAGACCAAG 
GCCATCAGCA TCTCCTGGAC GCCACCCATC GACTTTGATC CAGACCACCC ACTCTTGACA 
TCGGCCCTGC TGCAGCTCAG GGGGGATGTG AAGGAGACCT ACAAGGTCCT CTTTGTAGCC 
CAGGTGGTGA CCGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044664.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044726.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu66181
Clone ID Related Accession (Same CDS sequence)	XM_006044664.2
Accession Version	XM_006044664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAT 
CCAGAAACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCA CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACGCCACC CATCGACTTT GATCCAGACC ACCCACTCTT GACATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044726.1
CDS	408..5342
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQVVLFEKKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044664.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044664.2

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGGTCG TGCTCTTTGA AAAGAAAGTG 
TCCCACCACA TCCTCCTGAA GGGCGCAGCC CGCGAGCACA TGATGTTCGT GGAGGGCGGA 
GACCCCCTGG ACGTGCCCGT GGAGTCCCTG ACCGTGATCC CTGCCTTTGA CCTGGAGCAC 
AGAGAGGAAG CTGAGGAGCT AAAGCCCATC CTGGTGACCC TGGACTACGC CCAGCTGGAT 
CCAGAAACAC AGGCCCCACC TGCCACCCGA GAACTGCAGG TGGGCTGCAT CCGGACTACC 
CAGCTGTCCA CAAAGAAGAC TGTTGAGTTC AGCATGGACA ACATCGCAGC CCTGCAGCAC 
AAGGGCTTCA CCATTGAGCC CTCCAGAGGC TCTGTGGAGC GCGGCCAGAC CAAGGCCATC 
AGCATCTCCT GGACGCCACC CATCGACTTT GATCCAGACC ACCCACTCTT GACATCGGCC 
CTGCTGCAGC TCAGGGGGGA TGTGAAGGAG ACCTACAAGG TCCTCTTTGT AGCCCAGGTG 
GTGACCGGTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFAP74 ( XM_006044665.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044727.1 Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu66182
Clone ID Related Accession (Same CDS sequence)	XM_006044665.2
Accession Version	XM_006044665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cilia- and flagella-associated protein 74 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCAG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGG CCCTCCCTGT 
CCAGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGATCCA 
GAAACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCACAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CGCCACCCAT CGACTTTGAT CCAGACCACC CACTCTTGAC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044727.1
CDS	410..5341
Translation	MEEDNKKLLDDKPLMEDDTEELEDPELVLDCVPREAEQKAGLGSSCPGIEQSKDKQKQRLTAQEKRQACLLRRDLSHLDQLHEEKNLFIQKTREELRACRRRMELLIKQQESVAAEIAAERAANNMAAMGRLEAASQRLCSELKNERDLQAQMMVVLQQSESSLWQIEVQEGQLEDARKSAQEEAAAARRGLQERAAKQLHREMEASGKAEGNRLLRARRCVHLQKELGLRQQKLVEEAQKNHRKALRFLKASLGRIREQEQKEELETREHMRRRMDAVLALKNSISANRETLRKFQAWGQAKTELAEQKAQAEKKAILAQGGDAFKHLCHQRRRQELEAQNRAFEEEQKRRKQEIVSRLLKEEAMEDRRRARPEPPRPAGQRSLRDQTWSYVATFCDGQSTEAPLCCPLPDVPGQRPGPAQEQIKLQETRSRFCQSSPLLTAISSESVQGDGSLGGEDTLAWPEIPGLWNEDYKPFQVSKEDVDQKPVGGTKMDKDILARTMQRLRSGVIHKQVASGREFRGCPFNSKPKFIHFKDFDVGKVYKKKITLINATYTINYCKLVGVDESLQDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGSVSFLAQTGGFSVPLKCSTKKCSLSLDKELIDFGSYTVGETAIRTITLTNTGGLGTKFRFLLASETCEMDTSQSVLKLSSTFTYEDKSLYDKFTTSFSEHQMEGNESSPTDVPSQKESGKLDVKEQEEGHPIESEGVTLTTIMTIPPNEEQMEFSLGETTEGDIGPFSSIKVPVIFTPIVPGTVQNSFKVVFKNQQCPTLYFKVVGVAIDVPVWVPKPNVDLKICMYDRLYQDSILVHTRSKAALRLKFEVCKELRGHMELLPETGYIQALSSYSVQLKFLPRHSLPEDAGKYFDKETRVLEAPMTIRVADQIKPVGFTVHAIVTTSDLELSPAGLDFGYCTIYEAIRTQISLCNHSLLPQEFGFVGLPKFVDIQPNDGFGTILPLETLQLDVIFQPTKAKEYNFELICKSEINRCFKLACRAVGVYPPLELSHNQIKFAATSLYDTSVATLYVINSHLSMNSLTHSVPRIGSEDAFPVGPTSFEFLLPPDSPITISPLVGTVWPGKRCLVRVAFRPVLPSELIHQEAFQALSRKAEVKSSQKETVTQRKDQRRPSFSMLRLQNQDQLLRASASQPPELQKEDLASSSQEYQSAQASLTRSFQGKFDKFVVPCVVASGDTKDRKGTEPLSFSPHNTLYLELWCPAVAPSIVVTSDKGKTIINFGDIAAGHRGIKKVSIQNISPEDLDLEYSVLNPDGPFVLLNPTIRLPSGETQVLDLSFSPRESVVAQETLDIITKKGTLSLALMGTGVASTITCSIEGDVLNMGYVIARESVSLGFKLQNNSSLPIKFSMQLDSLSSRSRDQHRLPQFLASPDQRTEVVGTQNYSGQSVFSVVPVEGVMEPGKAQDFTVTFSPDHESLYFSDRLQKVSHHILLKGAAREHMMFVEGGDPLDVPVESLTVIPAFDLEHREGPPCPEAEELKPILVTLDYAQLDPETQAPPATRELQVGCIRTTQLSTKKTVEFSMDNIAALQHKGFTIEPSRGSVERGQTKAISISWTPPIDFDPDHPLLTSALLQLRGDVKETYKVLFVAQVVTGP

Target ORF information:

RefSeq Version	XM_006044665.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 74 (CFAP74), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044665.2

ORF Insert Sequence:

ATGGAAGAAG ACAACAAGAA GCTTTTGGAT GACAAGCCGC TCATGGAAGA TGACACAGAG 
GAGCTGGAGG ACCCAGAGCT TGTGCTGGAC TGTGTCCCCA GGGAGGCTGA GCAGAAGGCC 
GGCCTGGGAA GCTCCTGTCC AGGAATAGAG CAGAGCAAGG ACAAACAGAA ACAGAGGTTG 
ACTGCTCAGG AGAAAAGGCA GGCCTGCCTG CTGCGCAGGG ACTTGAGCCA CCTGGACCAG 
CTGCATGAAG AGAAAAACCT TTTCATCCAG AAGACAAGGG AGGAGCTACG TGCCTGCCGC 
AGGAGGATGG AACTCCTGAT CAAGCAGCAG GAGAGTGTGG CGGCCGAGAT AGCTGCAGAG 
AGGGCAGCCA ACAACATGGC AGCCATGGGC CGCCTGGAGG CAGCATCGCA GCGCCTGTGC 
TCGGAGCTGA AGAATGAGCG GGACCTGCAG GCGCAGATGA TGGTGGTGCT ACAGCAGAGC 
GAGAGCTCCC TGTGGCAGAT CGAGGTCCAG GAGGGGCAGC TAGAGGACGC CCGCAAGTCG 
GCCCAAGAGG AGGCTGCAGC CGCCAGGAGA GGCCTTCAGG AGCGGGCAGC CAAGCAGCTG 
CACCGGGAGA TGGAGGCCTC GGGGAAGGCA GAGGGGAACA GGCTGCTGCG GGCCCGGAGA 
TGCGTGCACC TGCAGAAGGA GCTTGGACTC CGGCAGCAGA AGCTGGTGGA GGAAGCCCAG 
AAGAACCACC GGAAGGCCCT GAGATTCCTG AAGGCCTCGT TGGGGAGGAT CCGTGAGCAG 
GAGCAGAAGG AGGAGCTGGA GACCCGAGAG CACATGCGCA GGCGCATGGA CGCGGTGCTG 
GCCCTGAAGA ACAGTATCTC CGCCAACCGG GAAACGCTCC GGAAATTTCA AGCATGGGGC 
CAAGCCAAGA CAGAGTTGGC AGAACAGAAG GCCCAGGCCG AGAAGAAGGC AATTCTGGCC 
CAAGGTGGGG ACGCCTTTAA GCACCTGTGC CATCAGCGCC GGCGCCAGGA GTTGGAGGCC 
CAGAACCGCG CCTTCGAGGA GGAGCAGAAG CGCAGGAAGC AGGAGATTGT GAGTCGGCTC 
TTGAAGGAAG AGGCCATGGA GGACCGGAGG AGGGCACGGC CCGAGCCCCC CAGACCCGCA 
GGCCAGCGGA GCCTGAGGGA CCAGACCTGG AGCTACGTGG CCACCTTCTG CGATGGGCAG 
AGCACAGAGG CCCCGCTCTG CTGTCCCCTG CCTGATGTCC CTGGACAGAG GCCAGGTCCA 
GCCCAAGAGC AGATAAAGCT CCAGGAGACC AGGTCCAGAT TCTGCCAGTC CTCTCCATTG 
CTGACGGCCA TCTCCAGTGA GTCTGTCCAG GGGGACGGGA GCCTCGGCGG GGAGGACACA 
CTGGCTTGGC CGGAGATCCC CGGGCTCTGG AATGAGGACT ACAAGCCTTT CCAGGTATCC 
AAGGAGGATG TGGACCAGAA GCCGGTGGGT GGAACAAAGA TGGACAAGGA CATCCTGGCG 
CGGACAATGC AACGACTACG CAGTGGGGTG ATCCACAAAC AGGTGGCATC AGGGCGTGAG 
TTCAGAGGGT GCCCCTTCAA CAGCAAGCCC AAGTTCATCC ACTTCAAGGA CTTTGATGTG 
GGCAAAGTGT ACAAGAAGAA GATCACGCTG ATCAATGCCA CCTACACGAT TAACTACTGC 
AAGCTGGTGG GCGTGGACGA GAGCCTGCAG GACTTCATCC ACGTGGACTT TGACCCCCCG 
GGCCCACTAT CAGCCGGCAT GTCCTGCGAA GTGCTTGTCA CCTTCAAGCC CATGATAAAC 
AAAGATCTGG AAGGAAGTGT CTCGTTTCTG GCTCAGACAG GCGGATTTTC TGTTCCACTG 
AAATGTTCAA CAAAGAAATG CTCTCTGTCT CTCGACAAGG AGCTCATCGA CTTTGGCAGC 
TACACAGTGG GTGAGACTGC AATCCGGACC ATCACGCTGA CCAACACTGG GGGCTTGGGC 
ACCAAGTTCA GATTTCTTCT GGCATCAGAG ACTTGTGAGA TGGACACCTC CCAGTCGGTC 
CTAAAATTAA GTAGTACCTT CACGTATGAA GACAAAAGTT TGTATGACAA ATTCACGACC 
AGTTTTTCTG AGCATCAGAT GGAGGGCAAT GAGTCATCTC CTACAGATGT ACCAAGCCAG 
AAGGAGTCTG GAAAGCTGGA CGTGAAGGAG CAGGAGGAGG GGCACCCCAT CGAATCAGAG 
GGCGTGACCT TGACCACCAT CATGACTATA CCTCCCAATG AGGAACAGAT GGAGTTCTCG 
CTGGGAGAGA CAACAGAGGG GGACATAGGC CCCTTCAGCT CCATCAAGGT GCCCGTCATC 
TTCACCCCGA TTGTCCCGGG AACGGTCCAG AACAGTTTCA AGGTTGTGTT TAAGAATCAG 
CAGTGCCCAA CTTTATATTT CAAAGTCGTT GGCGTGGCTA TCGATGTGCC TGTCTGGGTA 
CCAAAGCCCA ACGTAGACCT GAAGATCTGC ATGTACGATC GGCTGTACCA GGACTCGATC 
CTCGTCCACA CACGGTCGAA GGCCGCGTTG CGCCTAAAGT TCGAGGTGTG CAAGGAGCTG 
CGGGGCCACA TGGAGCTCCT GCCTGAGACT GGCTACATCC AGGCCCTGTC GTCCTACTCA 
GTGCAGCTCA AGTTCCTGCC CAGACACTCC CTCCCAGAAG ACGCAGGGAA GTATTTTGAC 
AAGGAGACCA GGGTTCTGGA GGCCCCGATG ACTATCCGGG TGGCTGACCA GATCAAACCC 
GTGGGGTTTA CTGTCCATGC TATAGTCACC ACCTCGGACC TGGAGCTCAG CCCCGCAGGG 
CTGGACTTTG GCTACTGTAC CATCTACGAG GCCATCAGGA CCCAAATCAG CCTCTGCAAT 
CATTCCCTCC TGCCCCAGGA GTTTGGGTTC GTCGGACTCC CCAAGTTTGT GGACATCCAG 
CCCAACGACG GGTTCGGGAC AATCCTGCCA CTGGAAACGC TACAGCTCGA TGTGATCTTT 
CAGCCCACCA AGGCCAAGGA GTACAACTTT GAGCTCATCT GCAAGTCAGA GATTAATCGG 
TGCTTCAAGC TGGCATGCCG GGCCGTGGGT GTCTACCCGC CCCTGGAGCT GTCCCACAAC 
CAGATCAAGT TTGCAGCCAC CTCCTTGTAC GACACATCCG TGGCCACCCT GTATGTCATC 
AACTCCCACT TGAGCATGAA CTCACTGACC CACTCAGTGC CCCGCATTGG CTCGGAGGAT 
GCTTTTCCCG TGGGGCCCAC GTCTTTTGAG TTCCTGCTGC CCCCAGATTC GCCCATCACC 
ATCTCACCCC TGGTGGGCAC CGTGTGGCCA GGAAAGAGGT GCCTGGTCCG AGTGGCCTTC 
CGGCCAGTGC TGCCCAGCGA GCTGATCCAC CAGGAGGCCT TCCAGGCCCT GAGCAGAAAA 
GCTGAGGTCA AATCATCCCA AAAGGAGACG GTCACCCAGA GGAAGGACCA GCGGAGACCA 
TCTTTCTCCA TGCTGCGGCT GCAGAACCAG GACCAGCTGC TCCGGGCCTC AGCCTCCCAG 
CCTCCGGAGC TGCAGAAGGA GGACCTCGCA TCCAGCTCTC AAGAGTACCA GTCGGCACAG 
GCCTCCCTGA CCAGGTCTTT CCAGGGGAAG TTTGACAAGT TTGTGGTTCC CTGTGTTGTT 
GCTAGTGGCG ACACCAAAGA CAGGAAGGGG ACAGAACCGC TGAGCTTCAG TCCCCACAAC 
ACGCTGTACC TGGAGCTGTG GTGTCCAGCG GTGGCGCCGT CTATCGTGGT GACGTCTGAC 
AAAGGCAAGA CCATCATTAA CTTCGGCGAC ATTGCTGCAG GACACCGGGG CATAAAGAAG 
GTCTCCATCC AGAACATCTC CCCTGAGGAT CTCGACCTGG AGTACTCCGT GCTGAACCCC 
GATGGCCCCT TCGTCCTGCT GAACCCCACC ATCAGGTTGC CCTCCGGCGA GACCCAGGTC 
CTAGATCTGT CCTTCTCCCC ACGTGAGAGT GTCGTGGCCC AAGAAACACT GGACATCATC 
ACCAAGAAAG GCACCCTCTC CCTGGCCCTC ATGGGCACGG GTGTGGCGTC CACGATCACG 
TGCTCCATTG AAGGTGATGT CCTCAACATG GGCTACGTGA TTGCCAGAGA GTCTGTCTCC 
TTGGGCTTCA AGCTGCAGAA CAACTCCTCA CTGCCCATCA AGTTCTCCAT GCAGCTGGAC 
AGCCTCTCCT CCAGGAGCAG AGACCAGCAC CGCCTGCCAC AGTTCCTGGC CTCGCCTGAC 
CAGAGGACGG AAGTCGTGGG CACGCAGAAC TACAGTGGCC AGAGCGTGTT CAGCGTGGTC 
CCGGTCGAGG GTGTCATGGA GCCTGGCAAG GCTCAGGACT TCACTGTGAC CTTCAGCCCA 
GACCACGAGA GCCTGTACTT CTCTGACCGG CTGCAGAAAG TGTCCCACCA CATCCTCCTG 
AAGGGCGCAG CCCGCGAGCA CATGATGTTC GTGGAGGGCG GAGACCCCCT GGACGTGCCC 
GTGGAGTCCC TGACCGTGAT CCCTGCCTTT GACCTGGAGC ACAGAGAGGG CCCTCCCTGT 
CCAGAAGCTG AGGAGCTAAA GCCCATCCTG GTGACCCTGG ACTACGCCCA GCTGGATCCA 
GAAACACAGG CCCCACCTGC CACCCGAGAA CTGCAGGTGG GCTGCATCCG GACTACCCAG 
CTGTCCACAA AGAAGACTGT TGAGTTCAGC ATGGACAACA TCGCAGCCCT GCAGCACAAG 
GGCTTCACCA TTGAGCCCTC CAGAGGCTCT GTGGAGCGCG GCCAGACCAA GGCCATCAGC 
ATCTCCTGGA CGCCACCCAT CGACTTTGAT CCAGACCACC CACTCTTGAC ATCGGCCCTG 
CTGCAGCTCA GGGGGGATGT GAAGGAGACC TACAAGGTCC TCTTTGTAGC CCAGGTGGTG 
ACCGGTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CFAP74
Entrez Gene ID	102405326
Full Name	cilia and flagella associated protein 74
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CFAP74 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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