CHD4 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006065803.1	XP_006065865.1	chromodomain-helicase-DNA-binding protein 4 isoform X3
XM_025283081.1	XP_025138866.1	chromodomain-helicase-DNA-binding protein 4 isoform X7
XM_025283077.1	XP_025138862.1	chromodomain-helicase-DNA-binding protein 4 isoform X3
XM_025283079.1	XP_025138864.1	chromodomain-helicase-DNA-binding protein 4 isoform X5
XM_006065802.1	XP_006065864.1	chromodomain-helicase-DNA-binding protein 4 isoform X2
XM_025283076.1	XP_025138861.1	chromodomain-helicase-DNA-binding protein 4 isoform X2
XM_025283080.1	XP_025138865.1	chromodomain-helicase-DNA-binding protein 4 isoform X6
XM_025283074.1	XP_025138859.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_006065801.1	XP_006065863.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_006065804.1	XP_006065866.1	chromodomain-helicase-DNA-binding protein 4 isoform X4
XM_025283075.1	XP_025138860.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_025283078.1	XP_025138863.1	chromodomain-helicase-DNA-binding protein 4 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu21263	XM_025283077.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21263	XM_006065801.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21264	XM_006065802.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21264	XM_025283080.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21265	XM_006065803.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21266	XM_006065804.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu64024	XM_025283075.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu64024	XM_025283074.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu64025	XM_025283076.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu64026	XM_025283078.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu64027	XM_025283079.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu64028	XM_025283081.1 Latest version!	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

CHD4 ( XM_025283077.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138862.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu21263
Clone ID Related Accession (Same CDS sequence)	XM_025283077.1 , XM_006065801.1
Accession Version	XM_025283077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGTTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138862.1
CDS	1..5739
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283077.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283077.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGTTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006065801.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065863.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

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CloneID	OBu21263
Clone ID Related Accession (Same CDS sequence)	XM_025283077.1 , XM_006065801.1
Accession Version	XM_006065801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGTTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065863.1
CDS	229..5967
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006065801.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065801.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGTTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006065802.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065864.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

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CloneID	OBu21264
Clone ID Related Accession (Same CDS sequence)	XM_006065802.1 , XM_025283080.1
Accession Version	XM_006065802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTA GAAGAACTGC TGAGTGACAT GAAAGCCGAT 
GTGACTCGAC TCCCAGCCAC TATTGCCCGA ATCCCCCCAG TTGCTGTGAG GCTACAGATG 
TCAGAGCGTA ATATTCTCAG TCGCCTGGCA AACCGGGCGC CTGAACCACC TCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065864.1
CDS	229..5946
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006065802.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065802.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTA GAAGAACTGC TGAGTGACAT GAAAGCCGAT 
GTGACTCGAC TCCCAGCCAC TATTGCCCGA ATCCCCCCAG TTGCTGTGAG GCTACAGATG 
TCAGAGCGTA ATATTCTCAG TCGCCTGGCA AACCGGGCGC CTGAACCACC TCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283080.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138865.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu21264
Clone ID Related Accession (Same CDS sequence)	XM_006065802.1 , XM_025283080.1
Accession Version	XM_025283080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTA GAAGAACTGC TGAGTGACAT GAAAGCCGAT 
GTGACTCGAC TCCCAGCCAC TATTGCCCGA ATCCCCCCAG TTGCTGTGAG GCTACAGATG 
TCAGAGCGTA ATATTCTCAG TCGCCTGGCA AACCGGGCGC CTGAACCACC TCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138865.1
CDS	1..5718
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283080.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283080.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTA GAAGAACTGC TGAGTGACAT GAAAGCCGAT 
GTGACTCGAC TCCCAGCCAC TATTGCCCGA ATCCCCCCAG TTGCTGTGAG GCTACAGATG 
TCAGAGCGTA ATATTCTCAG TCGCCTGGCA AACCGGGCGC CTGAACCACC TCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006065803.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065865.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu21265
Clone ID Related Accession (Same CDS sequence)	XM_006065803.1
Accession Version	XM_006065803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTAGAAGAA 
CTGCTGAGTG ACATGAAAGC CGATGTGACT CGACTCCCAG CCACTATTGC CCGAATCCCC 
CCAGTTGCTG TGAGGCTACA GATGTCAGAG CGTAATATTC TCAGTCGCCT GGCAAACCGG 
GCGCCTGAAC CACCTCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065865.1
CDS	229..5910
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006065803.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065803.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTAGAAGAA 
CTGCTGAGTG ACATGAAAGC CGATGTGACT CGACTCCCAG CCACTATTGC CCGAATCCCC 
CCAGTTGCTG TGAGGCTACA GATGTCAGAG CGTAATATTC TCAGTCGCCT GGCAAACCGG 
GCGCCTGAAC CACCTCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006065804.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065866.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

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CloneID	OBu21266
Clone ID Related Accession (Same CDS sequence)	XM_006065804.1
Accession Version	XM_006065804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785176.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AAGAGGAGGT GGAACGGGAG 
ATCATAAAAC AGGAAGAAAG TGTGGATCCC GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTGGCCCGA AACCTGGGCA AAGGAAAAAG AATTCGTAAA 
CAGGTCAACT ACAATGATGG CTCGCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTATTCGGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGTTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTTCTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGCTAT GGGATGCCCC CTCAGGATGC TTTTACCACC 
CAGTGGCTCG TGCGGGATCT GCGAGGCAAA TCGGAGAAGG AGTTCAAGGC TTATGTCTCG 
CTTTTTATGC GGCATTTATG TGAGCCGGGA GCAGATGGGG CCGAGACCTT TGCTGATGGC 
GTTCCCCGAG AAGGCCTGTC TCGTCAGCAT GTCCTTACTC GGATTGGGGT TATGTCCTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAACGGGC GCTGGAGCAT GCCTGAACTC 
GCAGAAGTAG AGGAAAATAA GAAAATGTCC CAGCCAGGGT CACCGTCCCC AAAGACACCT 
ACCCCCTCCA CTCCTGGGGA CACACAACCC AATACTCCAG CTCCTGCCCC GCCTGCCGAG 
GATGGGATAA AAGTAGAGGA AAATAACCTC AAAGAAGAAG AGAGTGCAGA GGCAGAGAAG 
GAGGTGAAGT CTGCACCCCC TGAGGCCGCC GTTGAGTGTA CACAGCCCCC TGCCCCTGCC 
TCAGAGGAGG AGAAGGTCCC AGTGGAGCCT CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTCAAGG AGAGAACAGA GGAAGCGATG GAGACAGATC CCAAAGGTAA TGCTGATGTG 
GAGAAGGTGG AGGAGAAGGC AGCAGTCGAT CTGACTCCCA TTGTGGTGGA GGACAAAGAA 
GAGAAGAAAG AAGAGGAGGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAGCA GAAGAAAAAT ATTAAACAGC GTTTCATGTT CAACATCGCA 
GATGGTGGTT TTACGGAGCT GCACTCCCTG TGGCAGAATG AGGAGCGGGC AGCCACCGTC 
ACCAAGAAGA CTTACGAGAT CTGGCACCGA CGCCATGACT ACTGGCTACT GGCTGGCATC 
ATAAACCATG GCTATGCCCG TTGGCAGGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AACGAACCTT TCAAGGGGGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAGTTT 
CTAGCCCGAA GGTTCAAGCT CTTAGAACAA GCCCTGGTGA TTGAGGAGCA GCTGCGTCGG 
GCAGCTTACC TCAACATGTC AGAGGACCCT TCTCACCCTT CCATGGCCCT AAACACCCGC 
TTTGCTGAGG TGGAGTGTCT GGCAGAGAGT CATCAGCACC TGTCCAAGGA GTCCATGGCA 
GGAAACAAGC CAGCTAACGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065866.1
CDS	230..5908
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006065804.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065804.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AAGAGGAGGT GGAACGGGAG 
ATCATAAAAC AGGAAGAAAG TGTGGATCCC GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTGGCCCGA AACCTGGGCA AAGGAAAAAG AATTCGTAAA 
CAGGTCAACT ACAATGATGG CTCGCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTATTCGGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGTTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTTCTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGCTAT GGGATGCCCC CTCAGGATGC TTTTACCACC 
CAGTGGCTCG TGCGGGATCT GCGAGGCAAA TCGGAGAAGG AGTTCAAGGC TTATGTCTCG 
CTTTTTATGC GGCATTTATG TGAGCCGGGA GCAGATGGGG CCGAGACCTT TGCTGATGGC 
GTTCCCCGAG AAGGCCTGTC TCGTCAGCAT GTCCTTACTC GGATTGGGGT TATGTCCTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAACGGGC GCTGGAGCAT GCCTGAACTC 
GCAGAAGTAG AGGAAAATAA GAAAATGTCC CAGCCAGGGT CACCGTCCCC AAAGACACCT 
ACCCCCTCCA CTCCTGGGGA CACACAACCC AATACTCCAG CTCCTGCCCC GCCTGCCGAG 
GATGGGATAA AAGTAGAGGA AAATAACCTC AAAGAAGAAG AGAGTGCAGA GGCAGAGAAG 
GAGGTGAAGT CTGCACCCCC TGAGGCCGCC GTTGAGTGTA CACAGCCCCC TGCCCCTGCC 
TCAGAGGAGG AGAAGGTCCC AGTGGAGCCT CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTCAAGG AGAGAACAGA GGAAGCGATG GAGACAGATC CCAAAGGTAA TGCTGATGTG 
GAGAAGGTGG AGGAGAAGGC AGCAGTCGAT CTGACTCCCA TTGTGGTGGA GGACAAAGAA 
GAGAAGAAAG AAGAGGAGGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAGCA GAAGAAAAAT ATTAAACAGC GTTTCATGTT CAACATCGCA 
GATGGTGGTT TTACGGAGCT GCACTCCCTG TGGCAGAATG AGGAGCGGGC AGCCACCGTC 
ACCAAGAAGA CTTACGAGAT CTGGCACCGA CGCCATGACT ACTGGCTACT GGCTGGCATC 
ATAAACCATG GCTATGCCCG TTGGCAGGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AACGAACCTT TCAAGGGGGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAGTTT 
CTAGCCCGAA GGTTCAAGCT CTTAGAACAA GCCCTGGTGA TTGAGGAGCA GCTGCGTCGG 
GCAGCTTACC TCAACATGTC AGAGGACCCT TCTCACCCTT CCATGGCCCT AAACACCCGC 
TTTGCTGAGG TGGAGTGTCT GGCAGAGAGT CATCAGCACC TGTCCAAGGA GTCCATGGCA 
GGAAACAAGC CAGCTAACGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283074.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138859.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

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CloneID	OBu64024
Clone ID Related Accession (Same CDS sequence)	XM_025283074.1 , XM_025283075.1
Accession Version	XM_025283074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ATTCGGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGTTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTTCTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGCTATGGG 
ATGCCCCCTC AGGATGCTTT TACCACCCAG TGGCTCGTGC GGGATCTGCG AGGCAAATCG 
GAGAAGGAGT TCAAGGCTTA TGTCTCGCTT TTTATGCGGC ATTTATGTGA GCCGGGAGCA 
GATGGGGCCG AGACCTTTGC TGATGGCGTT CCCCGAGAAG GCCTGTCTCG TCAGCATGTC 
CTTACTCGGA TTGGGGTTAT GTCCTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AACGGGCGCT GGAGCATGCC TGAACTCGCA GAAGTAGAGG AAAATAAGAA AATGTCCCAG 
CCAGGGTCAC CGTCCCCAAA GACACCTACC CCCTCCACTC CTGGGGACAC ACAACCCAAT 
ACTCCAGCTC CTGCCCCGCC TGCCGAGGAT GGGATAAAAG TAGAGGAAAA TAACCTCAAA 
GAAGAAGAGA GTGCAGAGGC AGAGAAGGAG GTGAAGTCTG CACCCCCTGA GGCCGCCGTT 
GAGTGTACAC AGCCCCCTGC CCCTGCCTCA GAGGAGGAGA AGGTCCCAGT GGAGCCTCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTCAAGGAGA GAACAGAGGA AGCGATGGAG 
ACAGATCCCA AAGGTAATGC TGATGTGGAG AAGGTGGAGG AGAAGGCAGC AGTCGATCTG 
ACTCCCATTG TGGTGGAGGA CAAAGAAGAG AAGAAAGAAG AGGAGGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAGCAGAA GAAAAATATT 
AAACAGCGTT TCATGTTCAA CATCGCAGAT GGTGGTTTTA CGGAGCTGCA CTCCCTGTGG 
CAGAATGAGG AGCGGGCAGC CACCGTCACC AAGAAGACTT ACGAGATCTG GCACCGACGC 
CATGACTACT GGCTACTGGC TGGCATCATA AACCATGGCT ATGCCCGTTG GCAGGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAC GAACCTTTCA AGGGGGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAGTTTCTA GCCCGAAGGT TCAAGCTCTT AGAACAAGCC 
CTGGTGATTG AGGAGCAGCT GCGTCGGGCA GCTTACCTCA ACATGTCAGA GGACCCTTCT 
CACCCTTCCA TGGCCCTAAA CACCCGCTTT GCTGAGGTGG AGTGTCTGGC AGAGAGTCAT 
CAGCACCTGT CCAAGGAGTC CATGGCAGGA AACAAGCCAG CTAACGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138859.1
CDS	251..6073
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283074.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283074.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ATTCGGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGTTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTTCTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGCTATGGG 
ATGCCCCCTC AGGATGCTTT TACCACCCAG TGGCTCGTGC GGGATCTGCG AGGCAAATCG 
GAGAAGGAGT TCAAGGCTTA TGTCTCGCTT TTTATGCGGC ATTTATGTGA GCCGGGAGCA 
GATGGGGCCG AGACCTTTGC TGATGGCGTT CCCCGAGAAG GCCTGTCTCG TCAGCATGTC 
CTTACTCGGA TTGGGGTTAT GTCCTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AACGGGCGCT GGAGCATGCC TGAACTCGCA GAAGTAGAGG AAAATAAGAA AATGTCCCAG 
CCAGGGTCAC CGTCCCCAAA GACACCTACC CCCTCCACTC CTGGGGACAC ACAACCCAAT 
ACTCCAGCTC CTGCCCCGCC TGCCGAGGAT GGGATAAAAG TAGAGGAAAA TAACCTCAAA 
GAAGAAGAGA GTGCAGAGGC AGAGAAGGAG GTGAAGTCTG CACCCCCTGA GGCCGCCGTT 
GAGTGTACAC AGCCCCCTGC CCCTGCCTCA GAGGAGGAGA AGGTCCCAGT GGAGCCTCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTCAAGGAGA GAACAGAGGA AGCGATGGAG 
ACAGATCCCA AAGGTAATGC TGATGTGGAG AAGGTGGAGG AGAAGGCAGC AGTCGATCTG 
ACTCCCATTG TGGTGGAGGA CAAAGAAGAG AAGAAAGAAG AGGAGGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAGCAGAA GAAAAATATT 
AAACAGCGTT TCATGTTCAA CATCGCAGAT GGTGGTTTTA CGGAGCTGCA CTCCCTGTGG 
CAGAATGAGG AGCGGGCAGC CACCGTCACC AAGAAGACTT ACGAGATCTG GCACCGACGC 
CATGACTACT GGCTACTGGC TGGCATCATA AACCATGGCT ATGCCCGTTG GCAGGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAC GAACCTTTCA AGGGGGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAGTTTCTA GCCCGAAGGT TCAAGCTCTT AGAACAAGCC 
CTGGTGATTG AGGAGCAGCT GCGTCGGGCA GCTTACCTCA ACATGTCAGA GGACCCTTCT 
CACCCTTCCA TGGCCCTAAA CACCCGCTTT GCTGAGGTGG AGTGTCTGGC AGAGAGTCAT 
CAGCACCTGT CCAAGGAGTC CATGGCAGGA AACAAGCCAG CTAACGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283075.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138860.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

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CloneID	OBu64024
Clone ID Related Accession (Same CDS sequence)	XM_025283074.1 , XM_025283075.1
Accession Version	XM_025283075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ATTCGGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGTTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTTCTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGCTATGGG 
ATGCCCCCTC AGGATGCTTT TACCACCCAG TGGCTCGTGC GGGATCTGCG AGGCAAATCG 
GAGAAGGAGT TCAAGGCTTA TGTCTCGCTT TTTATGCGGC ATTTATGTGA GCCGGGAGCA 
GATGGGGCCG AGACCTTTGC TGATGGCGTT CCCCGAGAAG GCCTGTCTCG TCAGCATGTC 
CTTACTCGGA TTGGGGTTAT GTCCTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AACGGGCGCT GGAGCATGCC TGAACTCGCA GAAGTAGAGG AAAATAAGAA AATGTCCCAG 
CCAGGGTCAC CGTCCCCAAA GACACCTACC CCCTCCACTC CTGGGGACAC ACAACCCAAT 
ACTCCAGCTC CTGCCCCGCC TGCCGAGGAT GGGATAAAAG TAGAGGAAAA TAACCTCAAA 
GAAGAAGAGA GTGCAGAGGC AGAGAAGGAG GTGAAGTCTG CACCCCCTGA GGCCGCCGTT 
GAGTGTACAC AGCCCCCTGC CCCTGCCTCA GAGGAGGAGA AGGTCCCAGT GGAGCCTCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTCAAGGAGA GAACAGAGGA AGCGATGGAG 
ACAGATCCCA AAGGTAATGC TGATGTGGAG AAGGTGGAGG AGAAGGCAGC AGTCGATCTG 
ACTCCCATTG TGGTGGAGGA CAAAGAAGAG AAGAAAGAAG AGGAGGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAGCAGAA GAAAAATATT 
AAACAGCGTT TCATGTTCAA CATCGCAGAT GGTGGTTTTA CGGAGCTGCA CTCCCTGTGG 
CAGAATGAGG AGCGGGCAGC CACCGTCACC AAGAAGACTT ACGAGATCTG GCACCGACGC 
CATGACTACT GGCTACTGGC TGGCATCATA AACCATGGCT ATGCCCGTTG GCAGGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAC GAACCTTTCA AGGGGGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAGTTTCTA GCCCGAAGGT TCAAGCTCTT AGAACAAGCC 
CTGGTGATTG AGGAGCAGCT GCGTCGGGCA GCTTACCTCA ACATGTCAGA GGACCCTTCT 
CACCCTTCCA TGGCCCTAAA CACCCGCTTT GCTGAGGTGG AGTGTCTGGC AGAGAGTCAT 
CAGCACCTGT CCAAGGAGTC CATGGCAGGA AACAAGCCAG CTAACGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138860.1
CDS	206..6028
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283075.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283075.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ATTCGGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGTTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTTCTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGCTATGGG 
ATGCCCCCTC AGGATGCTTT TACCACCCAG TGGCTCGTGC GGGATCTGCG AGGCAAATCG 
GAGAAGGAGT TCAAGGCTTA TGTCTCGCTT TTTATGCGGC ATTTATGTGA GCCGGGAGCA 
GATGGGGCCG AGACCTTTGC TGATGGCGTT CCCCGAGAAG GCCTGTCTCG TCAGCATGTC 
CTTACTCGGA TTGGGGTTAT GTCCTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AACGGGCGCT GGAGCATGCC TGAACTCGCA GAAGTAGAGG AAAATAAGAA AATGTCCCAG 
CCAGGGTCAC CGTCCCCAAA GACACCTACC CCCTCCACTC CTGGGGACAC ACAACCCAAT 
ACTCCAGCTC CTGCCCCGCC TGCCGAGGAT GGGATAAAAG TAGAGGAAAA TAACCTCAAA 
GAAGAAGAGA GTGCAGAGGC AGAGAAGGAG GTGAAGTCTG CACCCCCTGA GGCCGCCGTT 
GAGTGTACAC AGCCCCCTGC CCCTGCCTCA GAGGAGGAGA AGGTCCCAGT GGAGCCTCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTCAAGGAGA GAACAGAGGA AGCGATGGAG 
ACAGATCCCA AAGGTAATGC TGATGTGGAG AAGGTGGAGG AGAAGGCAGC AGTCGATCTG 
ACTCCCATTG TGGTGGAGGA CAAAGAAGAG AAGAAAGAAG AGGAGGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAGCAGAA GAAAAATATT 
AAACAGCGTT TCATGTTCAA CATCGCAGAT GGTGGTTTTA CGGAGCTGCA CTCCCTGTGG 
CAGAATGAGG AGCGGGCAGC CACCGTCACC AAGAAGACTT ACGAGATCTG GCACCGACGC 
CATGACTACT GGCTACTGGC TGGCATCATA AACCATGGCT ATGCCCGTTG GCAGGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAC GAACCTTTCA AGGGGGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAGTTTCTA GCCCGAAGGT TCAAGCTCTT AGAACAAGCC 
CTGGTGATTG AGGAGCAGCT GCGTCGGGCA GCTTACCTCA ACATGTCAGA GGACCCTTCT 
CACCCTTCCA TGGCCCTAAA CACCCGCTTT GCTGAGGTGG AGTGTCTGGC AGAGAGTCAT 
CAGCACCTGT CCAAGGAGTC CATGGCAGGA AACAAGCCAG CTAACGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283076.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138861.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

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CloneID	OBu64025
Clone ID Related Accession (Same CDS sequence)	XM_025283076.1
Accession Version	XM_025283076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTAGAAGAA 
CTGCTGAGTG ACATGAAAGC CGATGTGACT CGACTCCCAG CCACTATTGC CCGAATCCCC 
CCAGTTGCTG TGAGGCTACA GATGTCAGAG CGTAATATTC TCAGTCGCCT GGCAAACCGG 
GCGCCTGAAC CACCTCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138861.1
CDS	206..5947
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283076.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283076.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AGGAAGAGGA GGTGGAACGG 
GAGATCATAA AACAGGAAGA AAGTGTGGAT CCCGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTGGCC CGAAACCTGG GCAAAGGAAA AAGAATTCGT 
AAACAGGTCA ACTACAATGA TGGCTCGCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTATTC GGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGT 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTTC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGC TATGGGATGC CCCCTCAGGA TGCTTTTACC 
ACCCAGTGGC TCGTGCGGGA TCTGCGAGGC AAATCGGAGA AGGAGTTCAA GGCTTATGTC 
TCGCTTTTTA TGCGGCATTT ATGTGAGCCG GGAGCAGATG GGGCCGAGAC CTTTGCTGAT 
GGCGTTCCCC GAGAAGGCCT GTCTCGTCAG CATGTCCTTA CTCGGATTGG GGTTATGTCC 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAACG GGCGCTGGAG CATGCCTGAA 
CTCGCAGAAG TAGAGGAAAA TAAGAAAATG TCCCAGCCAG GGTCACCGTC CCCAAAGACA 
CCTACCCCCT CCACTCCTGG GGACACACAA CCCAATACTC CAGCTCCTGC CCCGCCTGCC 
GAGGATGGGA TAAAAGTAGA GGAAAATAAC CTCAAAGAAG AAGAGAGTGC AGAGGCAGAG 
AAGGAGGTGA AGTCTGCACC CCCTGAGGCC GCCGTTGAGT GTACACAGCC CCCTGCCCCT 
GCCTCAGAGG AGGAGAAGGT CCCAGTGGAG CCTCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTCA AGGAGAGAAC AGAGGAAGCG ATGGAGACAG ATCCCAAAGG TAATGCTGAT 
GTGGAGAAGG TGGAGGAGAA GGCAGCAGTC GATCTGACTC CCATTGTGGT GGAGGACAAA 
GAAGAGAAGA AAGAAGAGGA GGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA GCAGAAGAAA AATATTAAAC AGCGTTTCAT GTTCAACATC 
GCAGATGGTG GTTTTACGGA GCTGCACTCC CTGTGGCAGA ATGAGGAGCG GGCAGCCACC 
GTCACCAAGA AGACTTACGA GATCTGGCAC CGACGCCATG ACTACTGGCT ACTGGCTGGC 
ATCATAAACC ATGGCTATGC CCGTTGGCAG GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAACGAAC CTTTCAAGGG GGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAG 
TTTCTAGCCC GAAGGTTCAA GCTCTTAGAA CAAGCCCTGG TGATTGAGGA GCAGCTGCGT 
CGGGCAGCTT ACCTCAACAT GTCAGAGGAC CCTTCTCACC CTTCCATGGC CCTAAACACC 
CGCTTTGCTG AGGTGGAGTG TCTGGCAGAG AGTCATCAGC ACCTGTCCAA GGAGTCCATG 
GCAGGAAACA AGCCAGCTAA CGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTAGAAGAA 
CTGCTGAGTG ACATGAAAGC CGATGTGACT CGACTCCCAG CCACTATTGC CCGAATCCCC 
CCAGTTGCTG TGAGGCTACA GATGTCAGAG CGTAATATTC TCAGTCGCCT GGCAAACCGG 
GCGCCTGAAC CACCTCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283078.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138863.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

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CloneID	OBu64026
Clone ID Related Accession (Same CDS sequence)	XM_025283078.1
Accession Version	XM_025283078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AAGAGGAGGT GGAACGGGAG 
ATCATAAAAC AGGAAGAAAG TGTGGATCCC GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTGGCCCGA AACCTGGGCA AAGGAAAAAG AATTCGTAAA 
CAGGTCAACT ACAATGATGG CTCGCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTATTCGGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGTTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTTCTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGCTAT GGGATGCCCC CTCAGGATGC TTTTACCACC 
CAGTGGCTCG TGCGGGATCT GCGAGGCAAA TCGGAGAAGG AGTTCAAGGC TTATGTCTCG 
CTTTTTATGC GGCATTTATG TGAGCCGGGA GCAGATGGGG CCGAGACCTT TGCTGATGGC 
GTTCCCCGAG AAGGCCTGTC TCGTCAGCAT GTCCTTACTC GGATTGGGGT TATGTCCTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAACGGGC GCTGGAGCAT GCCTGAACTC 
GCAGAAGTAG AGGAAAATAA GAAAATGTCC CAGCCAGGGT CACCGTCCCC AAAGACACCT 
ACCCCCTCCA CTCCTGGGGA CACACAACCC AATACTCCAG CTCCTGCCCC GCCTGCCGAG 
GATGGGATAA AAGTAGAGGA AAATAACCTC AAAGAAGAAG AGAGTGCAGA GGCAGAGAAG 
GAGGTGAAGT CTGCACCCCC TGAGGCCGCC GTTGAGTGTA CACAGCCCCC TGCCCCTGCC 
TCAGAGGAGG AGAAGGTCCC AGTGGAGCCT CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTCAAGG AGAGAACAGA GGAAGCGATG GAGACAGATC CCAAAGGTAA TGCTGATGTG 
GAGAAGGTGG AGGAGAAGGC AGCAGTCGAT CTGACTCCCA TTGTGGTGGA GGACAAAGAA 
GAGAAGAAAG AAGAGGAGGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAGCA GAAGAAAAAT ATTAAACAGC GTTTCATGTT CAACATCGCA 
GATGGTGGTT TTACGGAGCT GCACTCCCTG TGGCAGAATG AGGAGCGGGC AGCCACCGTC 
ACCAAGAAGA CTTACGAGAT CTGGCACCGA CGCCATGACT ACTGGCTACT GGCTGGCATC 
ATAAACCATG GCTATGCCCG TTGGCAGGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AACGAACCTT TCAAGGGGGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAGTTT 
CTAGCCCGAA GGTTCAAGCT CTTAGAACAA GCCCTGGTGA TTGAGGAGCA GCTGCGTCGG 
GCAGCTTACC TCAACATGTC AGAGGACCCT TCTCACCCTT CCATGGCCCT AAACACCCGC 
TTTGCTGAGG TGGAGTGTCT GGCAGAGAGT CATCAGCACC TGTCCAAGGA GTCCATGGCA 
GGAAACAAGC CAGCTAACGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138863.1
CDS	206..5944
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283078.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283078.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGTG AGGTGTGCCA GCAAGGCGGA 
GAGATCATCC TGTGTGACAC GTGTCCCCGC GCATACCACA TGGTCTGCCT GGACCCGGAC 
ATGGAGAAGG CACCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAGGCAA AGGAGGACAA TTCGGAGGGC GAGGAGATCC TGGAGGAGGT TGGGGGAGAC 
CCCGAGGAAG AGGATGATCA CCACATGGAG TTCTGTCGGG TCTGCAAGGA TGGCGGGGAG 
CTGCTCTGCT GTGACACCTG TCCCTCCTCC TACCACATCC ACTGCCTGAA CCCCCCCCTC 
CCAGAGATCC CCAACGGCGA ATGGCTGTGT CCCCGCTGCA CGTGTCCAGC TCTCAAGGGC 
AAAGTTCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC GCCAGTGCCT 
CGGCCCCCAG ATGCTGATCC CAACACTCCG TCCCCCAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TCGTCAAGTG GCAAGGGATG TCGTACTGGC ACTGCTCCTG GGTGTCTGAG 
CTGCAGTTGG AGCTACACTG TCAAGTGATG TTCCGAAACT ATCAGCGCAA GAATGACATG 
GATGAACCAC CCTCTGGGGA CTTCGGTGGT GACGAGGAGA AGAGTCGGAA GCGGAAGAAC 
AAGGACCCTA AATTCGCTGA GATGGAGGAG CGCTTCTATC GCTATGGGAT AAAGCCCGAG 
TGGATGATGA TCCACCGAAT TCTCAACCAC AGCGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACCT ACCCTATGAC CAGGCATCCT GGGAGAGCGA GGATGTGGAG 
ATTCAGGACT ATGACCTGTT CAAGCAGAGC TACTGGAATC ACAGGGAGTT GATGAGGGGT 
GAGGAGGGAC GACCAGGCAA GAAGCTGAAG AAGGTGAAGC TGAGGAAGTT GGAGAGGCCC 
CCTGAAACCC CAACGGTTGA TCCAACAGTG AAGTATGAGC GCCAGCCAGA GTACCTGGAT 
GCTACGGGTG GGACCCTGCA CCCCTATCAG ATGGAAGGCC TGAACTGGCT ACGCTTCTCC 
TGGGCTCAGG GCACCGACAC CATCTTGGCC GACGAGATGG GCCTTGGGAA GACAGTGCAG 
ACTGCAGTCT TCCTGTACTC CCTTTACAAG GAGGGTCATT CCAAGGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAT 
ATGTACGTGG TGACCTACGT GGGTGACAAA GACAGCCGTG CTATCATCCG AGAGAATGAG 
TTCTCCTTTG AGGACAATGC CATTCGTGGC GGCAAGAAGG CTTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AGTTTCACGT GCTGCTGACA TCCTATGAGT TGATCACCAT TGACATGGCC 
ATCTTGGGCT CTATTGACTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTCAAGAAC 
AATCAGTCTA AGTTTTTCCG AGTTCTAAAT GGCTACTCAC TCCAGCACAA GCTGTTGCTG 
ACAGGGACTC CACTGCAGAA CAATCTAGAA GAGTTGTTCC ACCTGCTCAA CTTCCTCACC 
CCTGAGAGGT TCCACAATTT GGAAGGCTTC CTGGAGGAGT TTGCGGACAT TGCCAAGGAG 
GACCAGATTA AAAAGCTGCA CGACATGCTG GGCCCTCACA TGTTGCGGCG GCTCAAGGCT 
GACGTGTTCA AGAACATGCC GTCCAAGACA GAGCTGATCG TGCGTGTGGA GCTGAGCCCC 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGGAATT TTGAAGCCCT CAATGCCCGA 
GGGGGTGGCA ACCAGGTCTC TCTGCTAAAC GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CACCCATATC TCTTCCCGGT GGCCGCCATG GAAGCCCCTA AGATGCCTAA CGGCATGTAT 
GACGGCAGTG CCCTCATCAG AGCATCCGGG AAATTATTGC TGCTTCAGAA GATGCTCAAG 
AACCTCAAGG AGGGTGGGCA CCGTGTCCTC ATCTTCTCCC AGATGACCAA GATGCTGGAC 
CTGCTAGAGG ACTTCCTGGA ACATGAAGGT TATAAATATG AACGTATTGA TGGGGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAC CGCTTCAATG CACCAGGTGC TCAGCAGTTT 
TGCTTCCTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCTAC TGCTGACACA 
GTTATTATCT ACGACTCTGA TTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCT 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATTTATCGGT TTGTGACCCG TGCGTCTGTG 
GAAGAGCGCA TCACGCAGGT GGCCAAGAAA AAGATGATGC TGACGCATCT CGTTGTTCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCCATG TCCAAACAGG AGCTTGATGA TATCCTCAAG 
TTTGGCACCG AGGAGCTGTT CAAAGATGAG GCCACAGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGACA GCAGTGTCAT CCACTATGAT GATAAAGCCA TTGAACGACT GCTGGACCGG 
AACCAGGATG AGACTGAAGA CACGGAGCTA CAGGGCATGA ATGAATATTT GAGCTCCTTC 
AAAGTGGCCC AGTATGTGGT GCGAGAAGAA GAAATGGGGG AAGAGGAGGT GGAACGGGAG 
ATCATAAAAC AGGAAGAAAG TGTGGATCCC GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTGGCCCGA AACCTGGGCA AAGGAAAAAG AATTCGTAAA 
CAGGTCAACT ACAATGATGG CTCGCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTATTCGGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGTTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTTCTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGCTAT GGGATGCCCC CTCAGGATGC TTTTACCACC 
CAGTGGCTCG TGCGGGATCT GCGAGGCAAA TCGGAGAAGG AGTTCAAGGC TTATGTCTCG 
CTTTTTATGC GGCATTTATG TGAGCCGGGA GCAGATGGGG CCGAGACCTT TGCTGATGGC 
GTTCCCCGAG AAGGCCTGTC TCGTCAGCAT GTCCTTACTC GGATTGGGGT TATGTCCTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAACGGGC GCTGGAGCAT GCCTGAACTC 
GCAGAAGTAG AGGAAAATAA GAAAATGTCC CAGCCAGGGT CACCGTCCCC AAAGACACCT 
ACCCCCTCCA CTCCTGGGGA CACACAACCC AATACTCCAG CTCCTGCCCC GCCTGCCGAG 
GATGGGATAA AAGTAGAGGA AAATAACCTC AAAGAAGAAG AGAGTGCAGA GGCAGAGAAG 
GAGGTGAAGT CTGCACCCCC TGAGGCCGCC GTTGAGTGTA CACAGCCCCC TGCCCCTGCC 
TCAGAGGAGG AGAAGGTCCC AGTGGAGCCT CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTCAAGG AGAGAACAGA GGAAGCGATG GAGACAGATC CCAAAGGTAA TGCTGATGTG 
GAGAAGGTGG AGGAGAAGGC AGCAGTCGAT CTGACTCCCA TTGTGGTGGA GGACAAAGAA 
GAGAAGAAAG AAGAGGAGGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAGCA GAAGAAAAAT ATTAAACAGC GTTTCATGTT CAACATCGCA 
GATGGTGGTT TTACGGAGCT GCACTCCCTG TGGCAGAATG AGGAGCGGGC AGCCACCGTC 
ACCAAGAAGA CTTACGAGAT CTGGCACCGA CGCCATGACT ACTGGCTACT GGCTGGCATC 
ATAAACCATG GCTATGCCCG TTGGCAGGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AACGAACCTT TCAAGGGGGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAGTTT 
CTAGCCCGAA GGTTCAAGCT CTTAGAACAA GCCCTGGTGA TTGAGGAGCA GCTGCGTCGG 
GCAGCTTACC TCAACATGTC AGAGGACCCT TCTCACCCTT CCATGGCCCT AAACACCCGC 
TTTGCTGAGG TGGAGTGTCT GGCAGAGAGT CATCAGCACC TGTCCAAGGA GTCCATGGCA 
GGAAACAAGC CAGCTAACGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT AGAAGAACTG 
CTGAGTGACA TGAAAGCCGA TGTGACTCGA CTCCCAGCCA CTATTGCCCG AATCCCCCCA 
GTTGCTGTGA GGCTACAGAT GTCAGAGCGT AATATTCTCA GTCGCCTGGC AAACCGGGCG 
CCTGAACCAC CTCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283079.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138864.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

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CloneID	OBu64027
Clone ID Related Accession (Same CDS sequence)	XM_025283079.1
Accession Version	XM_025283079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGGTAT TCTGAAACAG CTAGAAGAAC TGCTGAGTGA CATGAAAGCC 
GATGTGACTC GACTCCCAGC CACTATTGCC CGAATCCCCC CAGTTGCTGT GAGGCTACAG 
ATGTCAGAGC GTAATATTCT CAGTCGCCTG GCAAACCGGG CGCCTGAACC ACCTCCACAG 
CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138864.1
CDS	206..5926
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283079.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283079.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCGG AGTTTGTGGA GGAGGAAGAA GAGGTGGCCC TGCGCTCAGA CAGCGAAGGC 
AGCGACTATA CCCCTGGTAA GAAGAAGAAG AAGAAGCTGG GCCCTAAGAA GGAGAAGAAG 
AGTAAATCCA AGCGGAAAGA GGAGGAGGAG GAGGACGAGG ATGACGACGA CTCAAAGGAG 
CCTAAGTCAT CAGCTCAGCT CTTGGAAGAC TGGGGCATGG AAGACATCGA CCATGTGTTC 
TCTGAGGAGG ATTATCGCAC CCTTACCAAC TACAAGGCCT TCAGCCAGTT TGTTAGACCC 
CTCATTGCTG CCAAAAACCC CAAGATCGCC GTCTCCAAGA TGATGATGGT TTTGGGTGCG 
AAGTGGCGGG AGTTCAGCAC CAACAATCCC TTCAAAGGCA GTTCTGGGGC TTCTGTGGCA 
GCAGCTGCAG CGGCAGCGGT GGCCGTGGTT GAGAGCATGG TGACGGCCAC TGAAGTGGCA 
CCGCCTCCAC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTCC CCGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCTT GAAAATCAAG CTGGGAGGTT TCGGTTCTAA GCGGAAGAGA 
TCCTCGAGTG AGGACGATGA CTTAGATGTG GAGTCTGACT TCGACGACGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCTACCAGT CGCAGTAGCC GCAGTCGCAA GAAACTGCGG 
ACCACAAAAA AGAAGAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGTGA GGTGTGCCAG CAAGGCGGAG AGATCATCCT GTGTGACACG 
TGTCCCCGCG CATACCACAT GGTCTGCCTG GACCCGGACA TGGAGAAGGC ACCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAGGCAAA GGAGGACAAT 
TCGGAGGGCG AGGAGATCCT GGAGGAGGTT GGGGGAGACC CCGAGGAAGA GGATGATCAC 
CACATGGAGT TCTGTCGGGT CTGCAAGGAT GGCGGGGAGC TGCTCTGCTG TGACACCTGT 
CCCTCCTCCT ACCACATCCA CTGCCTGAAC CCCCCCCTCC CAGAGATCCC CAACGGCGAA 
TGGCTGTGTC CCCGCTGCAC GTGTCCAGCT CTCAAGGGCA AAGTTCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACG CCAGTGCCTC GGCCCCCAGA TGCTGATCCC 
AACACTCCGT CCCCCAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT CGTCAAGTGG 
CAAGGGATGT CGTACTGGCA CTGCTCCTGG GTGTCTGAGC TGCAGTTGGA GCTACACTGT 
CAAGTGATGT TCCGAAACTA TCAGCGCAAG AATGACATGG ATGAACCACC CTCTGGGGAC 
TTCGGTGGTG ACGAGGAGAA GAGTCGGAAG CGGAAGAACA AGGACCCTAA ATTCGCTGAG 
ATGGAGGAGC GCTTCTATCG CTATGGGATA AAGCCCGAGT GGATGATGAT CCACCGAATT 
CTCAACCACA GCGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACCTA 
CCCTATGACC AGGCATCCTG GGAGAGCGAG GATGTGGAGA TTCAGGACTA TGACCTGTTC 
AAGCAGAGCT ACTGGAATCA CAGGGAGTTG ATGAGGGGTG AGGAGGGACG ACCAGGCAAG 
AAGCTGAAGA AGGTGAAGCT GAGGAAGTTG GAGAGGCCCC CTGAAACCCC AACGGTTGAT 
CCAACAGTGA AGTATGAGCG CCAGCCAGAG TACCTGGATG CTACGGGTGG GACCCTGCAC 
CCCTATCAGA TGGAAGGCCT GAACTGGCTA CGCTTCTCCT GGGCTCAGGG CACCGACACC 
ATCTTGGCCG ACGAGATGGG CCTTGGGAAG ACAGTGCAGA CTGCAGTCTT CCTGTACTCC 
CTTTACAAGG AGGGTCATTC CAAGGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGATA TGTACGTGGT GACCTACGTG 
GGTGACAAAG ACAGCCGTGC TATCATCCGA GAGAATGAGT TCTCCTTTGA GGACAATGCC 
ATTCGTGGCG GCAAGAAGGC TTCCCGCATG AAGAAAGAGG CATCTGTGAA GTTTCACGTG 
CTGCTGACAT CCTATGAGTT GATCACCATT GACATGGCCA TCTTGGGCTC TATTGACTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTCAAGAACA ATCAGTCTAA GTTTTTCCGA 
GTTCTAAATG GCTACTCACT CCAGCACAAG CTGTTGCTGA CAGGGACTCC ACTGCAGAAC 
AATCTAGAAG AGTTGTTCCA CCTGCTCAAC TTCCTCACCC CTGAGAGGTT CCACAATTTG 
GAAGGCTTCC TGGAGGAGTT TGCGGACATT GCCAAGGAGG ACCAGATTAA AAAGCTGCAC 
GACATGCTGG GCCCTCACAT GTTGCGGCGG CTCAAGGCTG ACGTGTTCAA GAACATGCCG 
TCCAAGACAG AGCTGATCGT GCGTGTGGAG CTGAGCCCCA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGGAATTT TGAAGCCCTC AATGCCCGAG GGGGTGGCAA CCAGGTCTCT 
CTGCTAAACG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ACCCATATCT CTTCCCGGTG 
GCCGCCATGG AAGCCCCTAA GATGCCTAAC GGCATGTATG ACGGCAGTGC CCTCATCAGA 
GCATCCGGGA AATTATTGCT GCTTCAGAAG ATGCTCAAGA ACCTCAAGGA GGGTGGGCAC 
CGTGTCCTCA TCTTCTCCCA GATGACCAAG ATGCTGGACC TGCTAGAGGA CTTCCTGGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGGGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGACC GCTTCAATGC ACCAGGTGCT CAGCAGTTTT GCTTCCTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCTACT GCTGACACAG TTATTATCTA CGACTCTGAT 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCTC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TTTATCGGTT TGTGACCCGT GCGTCTGTGG AAGAGCGCAT CACGCAGGTG 
GCCAAGAAAA AGATGATGCT GACGCATCTC GTTGTTCGGC CTGGGCTGGG CTCCAAGACT 
GGATCCATGT CCAAACAGGA GCTTGATGAT ATCCTCAAGT TTGGCACCGA GGAGCTGTTC 
AAAGATGAGG CCACAGATGG AGGAGGAGAC AACAAAGAGG GAGAAGACAG CAGTGTCATC 
CACTATGATG ATAAAGCCAT TGAACGACTG CTGGACCGGA ACCAGGATGA GACTGAAGAC 
ACGGAGCTAC AGGGCATGAA TGAATATTTG AGCTCCTTCA AAGTGGCCCA GTATGTGGTG 
CGAGAAGAAG AAATGGGGGA GGAAGAGGAG GTGGAACGGG AGATCATAAA ACAGGAAGAA 
AGTGTGGATC CCGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTGGCCC GAAACCTGGG CAAAGGAAAA AGAATTCGTA AACAGGTCAA CTACAATGAT 
GGCTCGCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTATTCG 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGTT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTTCT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGCT ATGGGATGCC CCCTCAGGAT GCTTTTACCA CCCAGTGGCT CGTGCGGGAT 
CTGCGAGGCA AATCGGAGAA GGAGTTCAAG GCTTATGTCT CGCTTTTTAT GCGGCATTTA 
TGTGAGCCGG GAGCAGATGG GGCCGAGACC TTTGCTGATG GCGTTCCCCG AGAAGGCCTG 
TCTCGTCAGC ATGTCCTTAC TCGGATTGGG GTTATGTCCT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAACGG GCGCTGGAGC ATGCCTGAAC TCGCAGAAGT AGAGGAAAAT 
AAGAAAATGT CCCAGCCAGG GTCACCGTCC CCAAAGACAC CTACCCCCTC CACTCCTGGG 
GACACACAAC CCAATACTCC AGCTCCTGCC CCGCCTGCCG AGGATGGGAT AAAAGTAGAG 
GAAAATAACC TCAAAGAAGA AGAGAGTGCA GAGGCAGAGA AGGAGGTGAA GTCTGCACCC 
CCTGAGGCCG CCGTTGAGTG TACACAGCCC CCTGCCCCTG CCTCAGAGGA GGAGAAGGTC 
CCAGTGGAGC CTCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTCAA GGAGAGAACA 
GAGGAAGCGA TGGAGACAGA TCCCAAAGGT AATGCTGATG TGGAGAAGGT GGAGGAGAAG 
GCAGCAGTCG ATCTGACTCC CATTGTGGTG GAGGACAAAG AAGAGAAGAA AGAAGAGGAG 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAG 
CAGAAGAAAA ATATTAAACA GCGTTTCATG TTCAACATCG CAGATGGTGG TTTTACGGAG 
CTGCACTCCC TGTGGCAGAA TGAGGAGCGG GCAGCCACCG TCACCAAGAA GACTTACGAG 
ATCTGGCACC GACGCCATGA CTACTGGCTA CTGGCTGGCA TCATAAACCA TGGCTATGCC 
CGTTGGCAGG ACATCCAGAA TGACCCACGC TATGCCATCC TCAACGAACC TTTCAAGGGG 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAGT TTCTAGCCCG AAGGTTCAAG 
CTCTTAGAAC AAGCCCTGGT GATTGAGGAG CAGCTGCGTC GGGCAGCTTA CCTCAACATG 
TCAGAGGACC CTTCTCACCC TTCCATGGCC CTAAACACCC GCTTTGCTGA GGTGGAGTGT 
CTGGCAGAGA GTCATCAGCA CCTGTCCAAG GAGTCCATGG CAGGAAACAA GCCAGCTAAC 
GCAGTCCTGC ACAAAGGTAT TCTGAAACAG CTAGAAGAAC TGCTGAGTGA CATGAAAGCC 
GATGTGACTC GACTCCCAGC CACTATTGCC CGAATCCCCC CAGTTGCTGT GAGGCTACAG 
ATGTCAGAGC GTAATATTCT CAGTCGCCTG GCAAACCGGG CGCCTGAACC ACCTCCACAG 
CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_025283081.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138866.1 Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.

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CloneID	OBu64028
Clone ID Related Accession (Same CDS sequence)	XM_025283081.1
Accession Version	XM_025283081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGACCACCA GGACTATTGT 
GAGGTGTGCC AGCAAGGCGG AGAGATCATC CTGTGTGACA CGTGTCCCCG CGCATACCAC 
ATGGTCTGCC TGGACCCGGA CATGGAGAAG GCACCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAGGCA AAGGAGGACA ATTCGGAGGG CGAGGAGATC 
CTGGAGGAGG TTGGGGGAGA CCCCGAGGAA GAGGATGATC ACCACATGGA GTTCTGTCGG 
GTCTGCAAGG ATGGCGGGGA GCTGCTCTGC TGTGACACCT GTCCCTCCTC CTACCACATC 
CACTGCCTGA ACCCCCCCCT CCCAGAGATC CCCAACGGCG AATGGCTGTG TCCCCGCTGC 
ACGTGTCCAG CTCTCAAGGG CAAAGTTCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CGCCAGTGCC TCGGCCCCCA GATGCTGATC CCAACACTCC GTCCCCCAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTCGTCAAGT GGCAAGGGAT GTCGTACTGG 
CACTGCTCCT GGGTGTCTGA GCTGCAGTTG GAGCTACACT GTCAAGTGAT GTTCCGAAAC 
TATCAGCGCA AGAATGACAT GGATGAACCA CCCTCTGGGG ACTTCGGTGG TGACGAGGAG 
AAGAGTCGGA AGCGGAAGAA CAAGGACCCT AAATTCGCTG AGATGGAGGA GCGCTTCTAT 
CGCTATGGGA TAAAGCCCGA GTGGATGATG ATCCACCGAA TTCTCAACCA CAGCGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACC TACCCTATGA CCAGGCATCC 
TGGGAGAGCG AGGATGTGGA GATTCAGGAC TATGACCTGT TCAAGCAGAG CTACTGGAAT 
CACAGGGAGT TGATGAGGGG TGAGGAGGGA CGACCAGGCA AGAAGCTGAA GAAGGTGAAG 
CTGAGGAAGT TGGAGAGGCC CCCTGAAACC CCAACGGTTG ATCCAACAGT GAAGTATGAG 
CGCCAGCCAG AGTACCTGGA TGCTACGGGT GGGACCCTGC ACCCCTATCA GATGGAAGGC 
CTGAACTGGC TACGCTTCTC CTGGGCTCAG GGCACCGACA CCATCTTGGC CGACGAGATG 
GGCCTTGGGA AGACAGTGCA GACTGCAGTC TTCCTGTACT CCCTTTACAA GGAGGGTCAT 
TCCAAGGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA TATGTACGTG GTGACCTACG TGGGTGACAA AGACAGCCGT 
GCTATCATCC GAGAGAATGA GTTCTCCTTT GAGGACAATG CCATTCGTGG CGGCAAGAAG 
GCTTCCCGCA TGAAGAAAGA GGCATCTGTG AAGTTTCACG TGCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGACATGGC CATCTTGGGC TCTATTGACT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTCAAGAA CAATCAGTCT AAGTTTTTCC GAGTTCTAAA TGGCTACTCA 
CTCCAGCACA AGCTGTTGCT GACAGGGACT CCACTGCAGA ACAATCTAGA AGAGTTGTTC 
CACCTGCTCA ACTTCCTCAC CCCTGAGAGG TTCCACAATT TGGAAGGCTT CCTGGAGGAG 
TTTGCGGACA TTGCCAAGGA GGACCAGATT AAAAAGCTGC ACGACATGCT GGGCCCTCAC 
ATGTTGCGGC GGCTCAAGGC TGACGTGTTC AAGAACATGC CGTCCAAGAC AGAGCTGATC 
GTGCGTGTGG AGCTGAGCCC CATGCAGAAG AAATACTACA AGTACATCCT CACTCGGAAT 
TTTGAAGCCC TCAATGCCCG AGGGGGTGGC AACCAGGTCT CTCTGCTAAA CGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCACCCATAT CTCTTCCCGG TGGCCGCCAT GGAAGCCCCT 
AAGATGCCTA ACGGCATGTA TGACGGCAGT GCCCTCATCA GAGCATCCGG GAAATTATTG 
CTGCTTCAGA AGATGCTCAA GAACCTCAAG GAGGGTGGGC ACCGTGTCCT CATCTTCTCC 
CAGATGACCA AGATGCTGGA CCTGCTAGAG GACTTCCTGG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGGGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA CCGCTTCAAT 
GCACCAGGTG CTCAGCAGTT TTGCTTCCTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCTA CTGCTGACAC AGTTATTATC TACGACTCTG ATTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC TCACCGTATT GGGCAAAATA AGAAGGTGAT GATTTATCGG 
TTTGTGACCC GTGCGTCTGT GGAAGAGCGC ATCACGCAGG TGGCCAAGAA AAAGATGATG 
CTGACGCATC TCGTTGTTCG GCCTGGGCTG GGCTCCAAGA CTGGATCCAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA GTTTGGCACC GAGGAGCTGT TCAAAGATGA GGCCACAGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAC AGCAGTGTCA TCCACTATGA TGATAAAGCC 
ATTGAACGAC TGCTGGACCG GAACCAGGAT GAGACTGAAG ACACGGAGCT ACAGGGCATG 
AATGAATATT TGAGCTCCTT CAAAGTGGCC CAGTATGTGG TGCGAGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTGGAACG GGAGATCATA AAACAGGAAG AAAGTGTGGA TCCCGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTGGC CCGAAACCTG 
GGCAAAGGAA AAAGAATTCG TAAACAGGTC AACTACAATG ATGGCTCGCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTATT CGGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG TTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTT 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG CTATGGGATG 
CCCCCTCAGG ATGCTTTTAC CACCCAGTGG CTCGTGCGGG ATCTGCGAGG CAAATCGGAG 
AAGGAGTTCA AGGCTTATGT CTCGCTTTTT ATGCGGCATT TATGTGAGCC GGGAGCAGAT 
GGGGCCGAGA CCTTTGCTGA TGGCGTTCCC CGAGAAGGCC TGTCTCGTCA GCATGTCCTT 
ACTCGGATTG GGGTTATGTC CTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAC 
GGGCGCTGGA GCATGCCTGA ACTCGCAGAA GTAGAGGAAA ATAAGAAAAT GTCCCAGCCA 
GGGTCACCGT CCCCAAAGAC ACCTACCCCC TCCACTCCTG GGGACACACA ACCCAATACT 
CCAGCTCCTG CCCCGCCTGC CGAGGATGGG ATAAAAGTAG AGGAAAATAA CCTCAAAGAA 
GAAGAGAGTG CAGAGGCAGA GAAGGAGGTG AAGTCTGCAC CCCCTGAGGC CGCCGTTGAG 
TGTACACAGC CCCCTGCCCC TGCCTCAGAG GAGGAGAAGG TCCCAGTGGA GCCTCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTC AAGGAGAGAA CAGAGGAAGC GATGGAGACA 
GATCCCAAAG GTAATGCTGA TGTGGAGAAG GTGGAGGAGA AGGCAGCAGT CGATCTGACT 
CCCATTGTGG TGGAGGACAA AGAAGAGAAG AAAGAAGAGG AGGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AGCAGAAGAA AAATATTAAA 
CAGCGTTTCA TGTTCAACAT CGCAGATGGT GGTTTTACGG AGCTGCACTC CCTGTGGCAG 
AATGAGGAGC GGGCAGCCAC CGTCACCAAG AAGACTTACG AGATCTGGCA CCGACGCCAT 
GACTACTGGC TACTGGCTGG CATCATAAAC CATGGCTATG CCCGTTGGCA GGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAACGAA CCTTTCAAGG GGGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA GTTTCTAGCC CGAAGGTTCA AGCTCTTAGA ACAAGCCCTG 
GTGATTGAGG AGCAGCTGCG TCGGGCAGCT TACCTCAACA TGTCAGAGGA CCCTTCTCAC 
CCTTCCATGG CCCTAAACAC CCGCTTTGCT GAGGTGGAGT GTCTGGCAGA GAGTCATCAG 
CACCTGTCCA AGGAGTCCAT GGCAGGAAAC AAGCCAGCTA ACGCAGTCCT GCACAAAGGT 
ATTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138866.1
CDS	206..5908
Translation	MASGLGSPSPCSAGSEEDDMDALLNNSLPPPHPENEEDPEEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEDEDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPAPPAEDGIKVEENNLKEEESAEAEKEVKSAPPEAAVECTQPPAPASEEEKVPVEPPEGEEKVEKAEVKERTEEAMETDPKGNADVEKVEEKAAVDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_025283081.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283081.1

ORF Insert Sequence:

ATGGCTTCGG GCCTGGGCTC CCCGTCCCCC TGCTCCGCGG GCAGCGAGGA GGACGACATG 
GATGCACTTT TGAACAACAG TCTGCCCCCA CCCCACCCAG AAAACGAAGA AGACCCAGAA 
GAGGACTTGT CAGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCG GAGTTTGTGG AGGAGGAAGA AGAGGTGGCC 
CTGCGCTCAG ACAGCGAAGG CAGCGACTAT ACCCCTGGTA AGAAGAAGAA GAAGAAGCTG 
GGCCCTAAGA AGGAGAAGAA GAGTAAATCC AAGCGGAAAG AGGAGGAGGA GGAGGACGAG 
GATGACGACG ACTCAAAGGA GCCTAAGTCA TCAGCTCAGC TCTTGGAAGA CTGGGGCATG 
GAAGACATCG ACCATGTGTT CTCTGAGGAG GATTATCGCA CCCTTACCAA CTACAAGGCC 
TTCAGCCAGT TTGTTAGACC CCTCATTGCT GCCAAAAACC CCAAGATCGC CGTCTCCAAG 
ATGATGATGG TTTTGGGTGC GAAGTGGCGG GAGTTCAGCA CCAACAATCC CTTCAAAGGC 
AGTTCTGGGG CTTCTGTGGC AGCAGCTGCA GCGGCAGCGG TGGCCGTGGT TGAGAGCATG 
GTGACGGCCA CTGAAGTGGC ACCGCCTCCA CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTC 
CCCGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCT TGAAAATCAA GCTGGGAGGT 
TTCGGTTCTA AGCGGAAGAG ATCCTCGAGT GAGGACGATG ACTTAGATGT GGAGTCTGAC 
TTCGACGACG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCTACCAG TCGCAGTAGC 
CGCAGTCGCA AGAAACTGCG GACCACAAAA AAGAAGAAGA AAGACCACCA GGACTATTGT 
GAGGTGTGCC AGCAAGGCGG AGAGATCATC CTGTGTGACA CGTGTCCCCG CGCATACCAC 
ATGGTCTGCC TGGACCCGGA CATGGAGAAG GCACCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAGGCA AAGGAGGACA ATTCGGAGGG CGAGGAGATC 
CTGGAGGAGG TTGGGGGAGA CCCCGAGGAA GAGGATGATC ACCACATGGA GTTCTGTCGG 
GTCTGCAAGG ATGGCGGGGA GCTGCTCTGC TGTGACACCT GTCCCTCCTC CTACCACATC 
CACTGCCTGA ACCCCCCCCT CCCAGAGATC CCCAACGGCG AATGGCTGTG TCCCCGCTGC 
ACGTGTCCAG CTCTCAAGGG CAAAGTTCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CGCCAGTGCC TCGGCCCCCA GATGCTGATC CCAACACTCC GTCCCCCAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTCGTCAAGT GGCAAGGGAT GTCGTACTGG 
CACTGCTCCT GGGTGTCTGA GCTGCAGTTG GAGCTACACT GTCAAGTGAT GTTCCGAAAC 
TATCAGCGCA AGAATGACAT GGATGAACCA CCCTCTGGGG ACTTCGGTGG TGACGAGGAG 
AAGAGTCGGA AGCGGAAGAA CAAGGACCCT AAATTCGCTG AGATGGAGGA GCGCTTCTAT 
CGCTATGGGA TAAAGCCCGA GTGGATGATG ATCCACCGAA TTCTCAACCA CAGCGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACC TACCCTATGA CCAGGCATCC 
TGGGAGAGCG AGGATGTGGA GATTCAGGAC TATGACCTGT TCAAGCAGAG CTACTGGAAT 
CACAGGGAGT TGATGAGGGG TGAGGAGGGA CGACCAGGCA AGAAGCTGAA GAAGGTGAAG 
CTGAGGAAGT TGGAGAGGCC CCCTGAAACC CCAACGGTTG ATCCAACAGT GAAGTATGAG 
CGCCAGCCAG AGTACCTGGA TGCTACGGGT GGGACCCTGC ACCCCTATCA GATGGAAGGC 
CTGAACTGGC TACGCTTCTC CTGGGCTCAG GGCACCGACA CCATCTTGGC CGACGAGATG 
GGCCTTGGGA AGACAGTGCA GACTGCAGTC TTCCTGTACT CCCTTTACAA GGAGGGTCAT 
TCCAAGGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA TATGTACGTG GTGACCTACG TGGGTGACAA AGACAGCCGT 
GCTATCATCC GAGAGAATGA GTTCTCCTTT GAGGACAATG CCATTCGTGG CGGCAAGAAG 
GCTTCCCGCA TGAAGAAAGA GGCATCTGTG AAGTTTCACG TGCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGACATGGC CATCTTGGGC TCTATTGACT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTCAAGAA CAATCAGTCT AAGTTTTTCC GAGTTCTAAA TGGCTACTCA 
CTCCAGCACA AGCTGTTGCT GACAGGGACT CCACTGCAGA ACAATCTAGA AGAGTTGTTC 
CACCTGCTCA ACTTCCTCAC CCCTGAGAGG TTCCACAATT TGGAAGGCTT CCTGGAGGAG 
TTTGCGGACA TTGCCAAGGA GGACCAGATT AAAAAGCTGC ACGACATGCT GGGCCCTCAC 
ATGTTGCGGC GGCTCAAGGC TGACGTGTTC AAGAACATGC CGTCCAAGAC AGAGCTGATC 
GTGCGTGTGG AGCTGAGCCC CATGCAGAAG AAATACTACA AGTACATCCT CACTCGGAAT 
TTTGAAGCCC TCAATGCCCG AGGGGGTGGC AACCAGGTCT CTCTGCTAAA CGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCACCCATAT CTCTTCCCGG TGGCCGCCAT GGAAGCCCCT 
AAGATGCCTA ACGGCATGTA TGACGGCAGT GCCCTCATCA GAGCATCCGG GAAATTATTG 
CTGCTTCAGA AGATGCTCAA GAACCTCAAG GAGGGTGGGC ACCGTGTCCT CATCTTCTCC 
CAGATGACCA AGATGCTGGA CCTGCTAGAG GACTTCCTGG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGGGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA CCGCTTCAAT 
GCACCAGGTG CTCAGCAGTT TTGCTTCCTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCTA CTGCTGACAC AGTTATTATC TACGACTCTG ATTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC TCACCGTATT GGGCAAAATA AGAAGGTGAT GATTTATCGG 
TTTGTGACCC GTGCGTCTGT GGAAGAGCGC ATCACGCAGG TGGCCAAGAA AAAGATGATG 
CTGACGCATC TCGTTGTTCG GCCTGGGCTG GGCTCCAAGA CTGGATCCAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA GTTTGGCACC GAGGAGCTGT TCAAAGATGA GGCCACAGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAC AGCAGTGTCA TCCACTATGA TGATAAAGCC 
ATTGAACGAC TGCTGGACCG GAACCAGGAT GAGACTGAAG ACACGGAGCT ACAGGGCATG 
AATGAATATT TGAGCTCCTT CAAAGTGGCC CAGTATGTGG TGCGAGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTGGAACG GGAGATCATA AAACAGGAAG AAAGTGTGGA TCCCGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTGGC CCGAAACCTG 
GGCAAAGGAA AAAGAATTCG TAAACAGGTC AACTACAATG ATGGCTCGCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTATT CGGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG TTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTT 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG CTATGGGATG 
CCCCCTCAGG ATGCTTTTAC CACCCAGTGG CTCGTGCGGG ATCTGCGAGG CAAATCGGAG 
AAGGAGTTCA AGGCTTATGT CTCGCTTTTT ATGCGGCATT TATGTGAGCC GGGAGCAGAT 
GGGGCCGAGA CCTTTGCTGA TGGCGTTCCC CGAGAAGGCC TGTCTCGTCA GCATGTCCTT 
ACTCGGATTG GGGTTATGTC CTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAC 
GGGCGCTGGA GCATGCCTGA ACTCGCAGAA GTAGAGGAAA ATAAGAAAAT GTCCCAGCCA 
GGGTCACCGT CCCCAAAGAC ACCTACCCCC TCCACTCCTG GGGACACACA ACCCAATACT 
CCAGCTCCTG CCCCGCCTGC CGAGGATGGG ATAAAAGTAG AGGAAAATAA CCTCAAAGAA 
GAAGAGAGTG CAGAGGCAGA GAAGGAGGTG AAGTCTGCAC CCCCTGAGGC CGCCGTTGAG 
TGTACACAGC CCCCTGCCCC TGCCTCAGAG GAGGAGAAGG TCCCAGTGGA GCCTCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTC AAGGAGAGAA CAGAGGAAGC GATGGAGACA 
GATCCCAAAG GTAATGCTGA TGTGGAGAAG GTGGAGGAGA AGGCAGCAGT CGATCTGACT 
CCCATTGTGG TGGAGGACAA AGAAGAGAAG AAAGAAGAGG AGGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AGCAGAAGAA AAATATTAAA 
CAGCGTTTCA TGTTCAACAT CGCAGATGGT GGTTTTACGG AGCTGCACTC CCTGTGGCAG 
AATGAGGAGC GGGCAGCCAC CGTCACCAAG AAGACTTACG AGATCTGGCA CCGACGCCAT 
GACTACTGGC TACTGGCTGG CATCATAAAC CATGGCTATG CCCGTTGGCA GGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAACGAA CCTTTCAAGG GGGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA GTTTCTAGCC CGAAGGTTCA AGCTCTTAGA ACAAGCCCTG 
GTGATTGAGG AGCAGCTGCG TCGGGCAGCT TACCTCAACA TGTCAGAGGA CCCTTCTCAC 
CCTTCCATGG CCCTAAACAC CCGCTTTGCT GAGGTGGAGT GTCTGGCAGA GAGTCATCAG 
CACCTGTCCA AGGAGTCCAT GGCAGGAAAC AAGCCAGCTA ACGCAGTCCT GCACAAAGGT 
ATTCTGAAAC AGCTAGAAGA ACTGCTGAGT GACATGAAAG CCGATGTGAC TCGACTCCCA 
GCCACTATTG CCCGAATCCC CCCAGTTGCT GTGAGGCTAC AGATGTCAGA GCGTAATATT 
CTCAGTCGCC TGGCAAACCG GGCGCCTGAA CCACCTCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CHD4
Entrez Gene ID	102404352
Full Name	chromodomain helicase DNA binding protein 4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CHD4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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