RASGEF1A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGEF1A
Entrez Gene ID	102403402
Full Name	RasGEF domain family member 1A
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006050749.1	XP_006050811.1	ras-GEF domain-containing family member 1A isoform X2
XM_006050748.2	XP_006050810.1	ras-GEF domain-containing family member 1A isoform X1
XM_006050750.2	XP_006050812.1	ras-GEF domain-containing family member 1A isoform X2
XM_006050748.1	XP_006050810.1	ras-GEF domain-containing family member 1A isoform X1
XM_006050751.1	XP_006050813.1	ras-GEF domain-containing family member 1A isoform X4
XM_006050751.2	XP_006050813.1	ras-GEF domain-containing family member 1A isoform X2
XM_025284574.1	XP_025140359.1	ras-GEF domain-containing family member 1A isoform X2
XM_006050750.1	XP_006050812.1	ras-GEF domain-containing family member 1A isoform X3

Pathways from BioSystems
Gene Ontology

The following RASGEF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGEF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu08770	XM_006050748.1 Latest version!	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu08771	XM_006050750.1 Latest version!	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu08771	XM_006050749.1 Latest version!	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu08771	XM_006050751.1 Latest version!	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu65220	XM_006050748.2 Latest version!	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBu65221	XM_006050751.2 Latest version!	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBu65221	XM_006050750.2 Latest version!	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBu65221	XM_025284574.1 Latest version!	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGEF1A ( XM_006050748.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050810.1 Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu08770
Clone ID Related Accession (Same CDS sequence)	XM_006050748.1
Accession Version	XM_006050748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GTTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACAC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG CGTGTGCTGT 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCCGAAG ACCTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGATA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTCAAGA CAGCAAAGTT CGACGTCTTA GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACGGC CAACAGCAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAACGGA CACATTAACT TCAAGAAATT CTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACAGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050810.1
CDS	106..1572
Translation	MFLEPQETMPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050748.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050748.1

ORF Insert Sequence:

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GTTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACAC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ACATCCTGGG CGTGTGCTGT 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCCGAAG ACCTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGATA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTCAAGA CAGCAAAGTT CGACGTCTTA GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACGGC CAACAGCAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAACGGA CACATTAACT TCAAGAAATT CTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACAGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050749.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050811.1 Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu08771
Clone ID Related Accession (Same CDS sequence)	XM_006050749.1 , XM_006050751.1 , XM_006050750.1
Accession Version	XM_006050749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050811.1
CDS	289..1731
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050749.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050749.1

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050751.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050813.1 Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu08771
Clone ID Related Accession (Same CDS sequence)	XM_006050749.1 , XM_006050751.1 , XM_006050750.1
Accession Version	XM_006050751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050813.1
CDS	105..1547
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050751.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050751.1

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050750.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050812.1 Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu08771
Clone ID Related Accession (Same CDS sequence)	XM_006050749.1 , XM_006050751.1 , XM_006050750.1
Accession Version	XM_006050750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784324.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050812.1
CDS	68..1510
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050750.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family, member 1A (RASGEF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050750.1

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGACAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050748.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050810.1 Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu65220
Clone ID Related Accession (Same CDS sequence)	XM_006050748.2
Accession Version	XM_006050748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GTTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACAC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ATATCCTGGG CGTGTGCTGT 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCCGAAG ACCTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGATA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTCAAGA CAGCAAAGTT CGACGTCTTA GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACGGC CAACAGCAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAACGGA CACATTAACT TCAAGAAATT CTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACAGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050810.1
CDS	134..1600
Translation	MFLEPQETMPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050748.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050748.2

ORF Insert Sequence:

ATGTTTCTGG AGCCCCAGGA AACTATGCCC CAGACGTCCG TGGTCTTCTC CAGCATCCTC 
GGGCCCAGCT GTAGTGGACA GGGGCAGCCC GGCATGGGGG AGCGAGGCGG TGGGGCCGGC 
GGCTCTGGGG ACCTCATCTT CCAAGATGGA CGTCTCATCT CGGGGTCCCT GGAGGCCCTG 
ATGGAGCACC TGGTCCCCAC AGTGGACTAT TACCCTGACA GGACGTACAT CTTCACGTTT 
CTCCTGAGCT CCCGGGTCTT CATCCCCCCT CATGACCTGC TGGCCCGCGT GGGGCAGCTC 
TGCGTGGAGC AGAGGCAGCA GTTGGAGGCT GGGTCCGAGA AGGCCAAGCT GAAGTCCTTC 
TCAGCCAAGA TCGTGCAGCT GCTTAAGGAG TGGACGGAGG CCTTCCCCTT TGACTTCCAG 
GACGAGAAGG CCATGGCTGA GTTGAAGGCC ATCACCCATC GGGTTACCCA GTGTGATGAG 
GAGAACGGCA CTGTGAAGAA GGCCATCTCC CAGATGACAC AGAGCCTGCT GCTGTCCCTG 
GCTGCCCGGA GCCAGCTTCA GGAGCTGCGG GAGAAGCTCC GCTCACCGGC CATGGACAAA 
GGGCCCGTCC TCAAGGCCAA GCCTCCAGCT GCTCAGAAGG ATATCCTGGG CGTGTGCTGT 
GACCCCCTGG TGCTGGCCCA GCAGCTGACA CATATCGAGC TGGAGAGAGT CAGCAGCATC 
CACCCCGAAG ACCTGATGCA GATTGTCAGC CACATGGACT CCCGGGACAA GCACAGGTGC 
CGAGGGGATA TGACCAAGAC GTACAGCCTG GAGGCCTATG ACAACTGGTT CAACTGCCTC 
AGCATGCTGG TGGCCACCGA GGTGTGCCGG GTAGTGAAGA AGAAGCACCG GACCCGCATG 
CTGGAGTTCT TCATTGACGT GGCCCGAGAG TGCTTCAACA TCGGCAACTT CAACTCCATG 
ATGGCCATCA TCTCTGGCAT GAACCTCAGT CCTGTGGCGA GGCTGAAGAA AACGTGGTCC 
AAGGTCAAGA CAGCAAAGTT CGACGTCTTA GAGCACCACA TGGACCCGTC CAGCAATTTC 
TGCAACTACC GGACAGCCCT GCAGGGGGCC ACGCAGAGGT CCCAGACGGC CAACAGCAGC 
CGGGAGAAGA TCGTCATCCC CGTGTTCAAC CTTTTCGTCA AGGACATCTA CTTCCTGCAC 
AAAATCCACA CCAACCACCT GCCCAACGGA CACATTAACT TCAAGAAATT CTGGGAGATC 
TCCAGGCAGA TCCATGAGTT CATGACATGG ACACAGGTGG AGTGTCCCTT TGAGAAGGAC 
AAGAAGATTC AGAGTTATCT GCTGACAGCT CCAATCTACA GCGAAGAAGC TCTCTTCATC 
GCCTCCTTTG AAAGTGAAGG TCCAGAAAAC CACATGGAAA AGGACAGCTG GAAGACCCTC 
AGGACCACTC TCCTTAACAG AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050750.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050812.1 Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu65221
Clone ID Related Accession (Same CDS sequence)	XM_006050750.2 , XM_006050751.2 , XM_025284574.1
Accession Version	XM_006050750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050812.1
CDS	107..1549
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050750.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050750.2

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_006050751.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050813.1 Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu65221
Clone ID Related Accession (Same CDS sequence)	XM_006050750.2 , XM_006050751.2 , XM_025284574.1
Accession Version	XM_006050751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050813.1
CDS	157..1599
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_006050751.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050751.2

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGEF1A ( XM_025284574.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025140359.1 Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

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CloneID	OBu65221
Clone ID Related Accession (Same CDS sequence)	XM_006050750.2 , XM_006050751.2 , XM_025284574.1
Accession Version	XM_025284574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras-GEF domain-containing family member 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025140359.1
CDS	383..1825
Translation	MPQTSVVFSSILGPSCSGQGQPGMGERGGGAGGSGDLIFQDGRLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFIPPHDLLARVGQLCVEQRQQLEAGSEKAKLKSFSAKIVQLLKEWTEAFPFDFQDEKAMAELKAITHRVTQCDEENGTVKKAISQMTQSLLLSLAARSQLQELREKLRSPAMDKGPVLKAKPPAAQKDILGVCCDPLVLAQQLTHIELERVSSIHPEDLMQIVSHMDSRDKHRCRGDMTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQTANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFIASFESEGPENHMEKDSWKTLRTTLLNRA

Target ORF information:

RefSeq Version	XM_025284574.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RasGEF domain family member 1A (RASGEF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025284574.1

ORF Insert Sequence:

ATGCCCCAGA CGTCCGTGGT CTTCTCCAGC ATCCTCGGGC CCAGCTGTAG TGGACAGGGG 
CAGCCCGGCA TGGGGGAGCG AGGCGGTGGG GCCGGCGGCT CTGGGGACCT CATCTTCCAA 
GATGGACGTC TCATCTCGGG GTCCCTGGAG GCCCTGATGG AGCACCTGGT CCCCACAGTG 
GACTATTACC CTGACAGGAC GTACATCTTC ACGTTTCTCC TGAGCTCCCG GGTCTTCATC 
CCCCCTCATG ACCTGCTGGC CCGCGTGGGG CAGCTCTGCG TGGAGCAGAG GCAGCAGTTG 
GAGGCTGGGT CCGAGAAGGC CAAGCTGAAG TCCTTCTCAG CCAAGATCGT GCAGCTGCTT 
AAGGAGTGGA CGGAGGCCTT CCCCTTTGAC TTCCAGGACG AGAAGGCCAT GGCTGAGTTG 
AAGGCCATCA CCCATCGGGT TACCCAGTGT GATGAGGAGA ACGGCACTGT GAAGAAGGCC 
ATCTCCCAGA TGACACAGAG CCTGCTGCTG TCCCTGGCTG CCCGGAGCCA GCTTCAGGAG 
CTGCGGGAGA AGCTCCGCTC ACCGGCCATG GACAAAGGGC CCGTCCTCAA GGCCAAGCCT 
CCAGCTGCTC AGAAGGATAT CCTGGGCGTG TGCTGTGACC CCCTGGTGCT GGCCCAGCAG 
CTGACACATA TCGAGCTGGA GAGAGTCAGC AGCATCCACC CCGAAGACCT GATGCAGATT 
GTCAGCCACA TGGACTCCCG GGACAAGCAC AGGTGCCGAG GGGATATGAC CAAGACGTAC 
AGCCTGGAGG CCTATGACAA CTGGTTCAAC TGCCTCAGCA TGCTGGTGGC CACCGAGGTG 
TGCCGGGTAG TGAAGAAGAA GCACCGGACC CGCATGCTGG AGTTCTTCAT TGACGTGGCC 
CGAGAGTGCT TCAACATCGG CAACTTCAAC TCCATGATGG CCATCATCTC TGGCATGAAC 
CTCAGTCCTG TGGCGAGGCT GAAGAAAACG TGGTCCAAGG TCAAGACAGC AAAGTTCGAC 
GTCTTAGAGC ACCACATGGA CCCGTCCAGC AATTTCTGCA ACTACCGGAC AGCCCTGCAG 
GGGGCCACGC AGAGGTCCCA GACGGCCAAC AGCAGCCGGG AGAAGATCGT CATCCCCGTG 
TTCAACCTTT TCGTCAAGGA CATCTACTTC CTGCACAAAA TCCACACCAA CCACCTGCCC 
AACGGACACA TTAACTTCAA GAAATTCTGG GAGATCTCCA GGCAGATCCA TGAGTTCATG 
ACATGGACAC AGGTGGAGTG TCCCTTTGAG AAGGACAAGA AGATTCAGAG TTATCTGCTG 
ACAGCTCCAA TCTACAGCGA AGAAGCTCTC TTCATCGCCT CCTTTGAAAG TGAAGGTCCA 
GAAAACCACA TGGAAAAGGA CAGCTGGAAG ACCCTCAGGA CCACTCTCCT TAACAGAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RASGEF1A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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